CN101550120B - 一种艾叶的有效组分及其制备方法 - Google Patents
一种艾叶的有效组分及其制备方法 Download PDFInfo
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- CN101550120B CN101550120B CN2008100526045A CN200810052604A CN101550120B CN 101550120 B CN101550120 B CN 101550120B CN 2008100526045 A CN2008100526045 A CN 2008100526045A CN 200810052604 A CN200810052604 A CN 200810052604A CN 101550120 B CN101550120 B CN 101550120B
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Abstract
Description
艾叶C01组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.11 | 0.35 | 0.07 | 0.12 |
抑制率(%) | 87.32 | 0 | 100 | 80.45 |
RSD(%) | 4.69 | 2.89 | 7.99 | 4.21 |
艾叶C02组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.11 | 0.35 | 0.07 | 0.12 |
抑制率(%) | 86.61 | 0 | 100 | 80.45 |
RSD(%) | 7.24 | 2.89 | 7.99 | 4.21 |
艾叶C05组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.11 | 0.35 | 0.07 | 0.12 |
抑制率(%) | 86.96 | 0 | 100 | 80.45 |
RSD(%) | 0.66 | 2.89 | 7.99 | 4.21 |
艾叶C06组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.09 | 0.35 | 0.07 | 0.12 |
抑制率(%) | 91.79 | 0 | 100 | 80.45 |
RSD(%) | 2.22 | 2.89 | 7.99 | 4.21 |
艾叶C07组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.11 | 0.34 | 0.06 | 0.09 |
抑制率(%) | 83.75 | 0 | 100 | 88.66 |
RSD(%) | 4.69 | 3.04 | 4.28 | 4.74 |
艾叶C09组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.11 | 0.34 | 0.06 | 0.09 |
抑制率(%) | 81.25 | 0 | 100 | 88.66 |
RSD(%) | 0.63 | 3.04 | 4.28 | 4.74 |
艾叶C02组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.29 | 0.59 | 0.16 | 0.17 |
抑制率(%) | 70.00 | 0 | 100 | 96.86 |
RSD(%) | 0.98 | 4.861 | 5.093 | 5.272 |
艾叶C04组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.27 | 0.59 | 0.16 | 0.17 |
抑制率(%) | 74.07 | 0 | 100 | 96.86 |
RSD(%) | 0.26 | 4.861 | 5.093 | 5.272 |
艾叶C05组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.24 | 0.59 | 0.16 | 0.17 |
抑制率(%) | 81.98 | 0 | 100 | 96.86 |
RSD(%) | 8.63 | 4.861 | 5.093 | 5.272 |
艾叶C06组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.15 | 0.59 | 0.16 | 0.17 |
抑制率(%) | 101.28 | 0 | 100 | 96.86 |
RSD(%) | 6.87 | 4.861 | 5.093 | 5.272 |
艾叶C09组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.18 | 0.59 | 0.16 | 0.17 |
抑制率(%) | 95.93 | 0 | 100 | 96.86 |
RSD(%) | 8.37 | 4.861 | 5.093 | 5.272 |
艾叶C01组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.13 | 0.69 | 0.04 | 0.12 |
抑制率(%) | 86.46 | 0 | 100 | 87.19 |
RSD(%) | 5.52 | 6.622 | 1.33 | 4.363 |
艾叶C02组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.13 | 0.69 | 0.04 | 0.12 |
抑制率(%) | 86.38 | 0 | 100 | 87.19 |
RSD(%) | 6.05 | 6.622 | 1.33 | 4.363 |
艾叶C05组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.09 | 0.69 | 0.04 | 0.12 |
抑制率(%) | 91.92 | 0 | 100 | 87.19 |
RSD(%) | 2.29 | 6.622 | 1.33 | 4.363 |
艾叶C06组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.09 | 0.69 | 0.04 | 0.12 |
抑制率(%) | 92.62 | 0 | 100 | 87.19 |
RSD(%) | 3.21 | 6.622 | 1.33 | 4.363 |
艾叶C07组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.09 | 0.69 | 0.04 | 0.12 |
抑制率(%) | 92.62 | 0 | 100 | 87.19 |
RSD(%) | 3.21 | 6.622 | 1.33 | 4.363 |
艾叶C09组分 | 阴性 | 空白 | 阳性 | |
平均细胞存活数 | 0.14 | 0.69 | 0.04 | 0.12 |
抑制率(%) | 84.77 | 0 | 100 | 87.19 |
RSD(%) | 2.03 | 6.622 | 1.33 | 4.363 |
Claims (3)
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CN1962698A (zh) * | 2006-11-17 | 2007-05-16 | 华东理工大学 | 艾叶多糖提取物的用途 |
CN101067004A (zh) * | 2007-06-07 | 2007-11-07 | 华东理工大学 | 一种艾叶多糖及其用途 |
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Non-Patent Citations (3)
Title |
---|
刘延庆 等.艾叶提取物抗肿瘤活性的体外实验研究.《中药材》.2006,第29卷(第11期),1213-1215. * |
尹庚明 等.艾叶挥发性成分的提取及其化学成分的气相色谱/质谱分析.《分析化学》.1999,第27卷(第1期),55-58. * |
徐新建 等.艾叶挥发油化学成分的气相色谱-质谱联用分析.《时珍国医国药》.2007,第18卷(第11期),2657-2658. * |
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