CN101539571A - Array-type 96-channel rapid detector for pesticide residue - Google Patents
Array-type 96-channel rapid detector for pesticide residue Download PDFInfo
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Abstract
The invention discloses an array-type 96-channel rapid detector for pesticide residue, which comprises a casing. A front panel of the casing is provided with an LCD screen and a plurality of keys; the upper surface of the casing is provided with a printer and a cover plate of a 96-channel sample-detection module; the rear part of the casing is provided with a power socket, a power switch and an RS232/USB interface; and the 96-channel sample-detection module, a microprocessor and a power source are arranged inside the casing, wherein the 96-channel sample-detection module of the 96-channel rapid detector for pesticide residue is formed by combining two 48-channel detection modules; a module light source is an array-type light source which integrates and combines 96 light-emitting diodes 412 nanometers in wavelength; a module detector is an integrated-type array combination of 96 photoelectric sensors; and the array-type light source and the array-type detector are in one-to-one correspondence of arrangement mode and cooperate with each other. The rapid detector can rapidly detect the content of organophosphorus and carbamate pesticide residue in foods, vegetables, fruits and water so as to guarantee the safety of eaters.
Description
Technical field
The present invention relates to a kind of easy, sensitive, analytical instrument fast, be specifically related to a kind of express-analysis instrument that on-the-spot fast quantification detects residues of pesticides content that is used for.
Background technology
Along with the deepening continuously and develop of economical operation globalization, fruits and vegetables chemistry security problem more and more receives the concern of national governments, international organization, industry organization, industry specialists and even general public.Residues of pesticides are meant the trace pesticide that remains in environment and the biosome, comprise the agricultural chemicals substance, stay metabolin, degradation product and impurity.Be applied to the agricultural chemicals on the crop, wherein a part is attached on the crop, and a part is scattered in the environment such as soil, big G﹠W, and the part in the remaining agricultural chemicals of environment again can be by plant absorbing.Remains of pesticide directly passes through fruit or water, atmosphere arrives in the people, animal body, or finally passes to people, animal by environment, food chain.The reason that causes and influence residues of pesticides has a lot, and wherein the using method of the character of agricultural chemicals itself, environmental factor and agricultural chemicals is the principal element that influences residues of pesticides.Edible contain a large amount of high poison, the residual food that causes of highly toxic pesticide can cause people, animal acute poisoning accident.The agricultural byproducts that long-term edible residues of pesticides exceed standard though can not cause acute poisoning, may cause the slow poisoning of humans and animals, cause the generation of disease, even have influence on the next generation.In view of this, Detecting Pesticide is very necessary.At present, existing Multiple Pesticides residue detection instrument.Yet, need simultaneously a plurality of samples to be done Detecting Pesticide for departments such as the administration for industry and commerce, vegetables production base, the market of farm produce, supermarket, quality inspections and could not incur loss through delay the vegetables currency.Therefore, press for a kind of instrument that can do a plurality of sample detection simultaneously on the market, at present, mostly adopt the hyperchannel instrument for detecting pesticide residue through of microplate reader repacking on the market, in 96 passages, must do blank the detection with 8 passages at every turn, so can only carry out 88 sample detection,, not only use and operate inconvenience because the mode of the ELISA Plate that employing 8 passages move in proper order detects, and ELISA Plate is in all the time and moves, and makes the consistance of testing result very poor.
Summary of the invention
The object of the present invention is to provide a kind of array-type 96-channel rapid detector for pesticide residue that on-the-spot fast quantification detects residues of pesticides content that is used for.
For reaching above-mentioned purpose, the concrete content of the present invention is:
A kind of array-type 96-channel rapid detector for pesticide residue comprises a casing and 96 passage ELISA Plate, light source, detecting device, microprocessor and power supply located therein;
Wherein 96 passage ELISA Plate, light source and detecting device are formed the detection system module; Described light source is the array light source of 96 the integrated combination of light emitting diode, the array detecting device that described detecting device is made of 96 photoelectric sensors, described array light source and array detecting device correspondence are assembled in the framework both sides of a placement 96 passage ELISA Plate; Array light source is corresponding one by one with the position of array detecting device, and maintains static.Make 96 passage sample detection modularizations, when detecting, need not transfer component locations.
Wherein said detection system module constitutes 96 passage sample detection modules by two 48 passage detection system module combinations.
Described detection system module is established a light-source system mainboard, establishes a light-source system pedestal above the light-source system mainboard, lays a plurality of light emitting diodes above the light-source system mainboard; If a detector system mainboard is placed in a detector system pedestal and ties face, establish the detecting device bottom outlet above the detector system pedestal, detecting device above the detector system mainboard and detector system pedestal are tied the detecting device bottom outlet clearance fit of face; The system framework of setting up departments in light-source system pedestal and the detector system pedestal, system framework is connected with detector system pedestal knot the light-source system pedestal by fastening bolt in the mode of closely cooperating.
Described light-source system pedestal knot is established the light-source system base plate, is uniform-distribution with light-source system LED bottom outlet on the light-source system base plate, and the size of light-source system LED bottom outlet and arrangement mode match with LED size and arrangement mode on the light-source system mainboard.
Described detector system pedestal knot comprises a detector system base plate, is uniform-distribution with the detector system bottom outlet on the detector system base plate, and LED size and arrangement mode match in the size of detector system bottom outlet and arrangement mode and the light-source system wiring board.
The inside of described system framework is provided with the dead slot of putting ELISA Plate, and described dead slot size matches with described ELISA Plate size.
Wherein on the detector system base plate, establish the detector system connecting hole, on the light-source system base plate, establish the light-source system connecting hole, light-source system pedestal, detector system pedestal and system framework are coupled together by detector system connecting hole and light-source system connecting hole.
Detector is by the super bright light emitting diode combined forming array formula light source of single wavelength 412nm.
Described casing front panel is provided with LCDs and a plurality of button, and described casing upper surface is provided with printer interface, 96 passage sample detection module cover, and described casing rear portion also is provided with supply socket, power switch and RS232/USB interface.
Described casing front panel is provided with LCDs and a plurality of button, and described casing upper surface is provided with printer interface, 96 passage sample detection module cover, and described casing rear portion also is provided with supply socket, power switch and RS232/USB interface.
The present invention adopts technique scheme, and its distinguishing feature is:
1, adopt the colorimetric analysis method, make easy and simple to handlely, the testing result intuitive is good.
2, be applicable to that the fast quantification to simple sample or a plurality of samples detects.
3, adopt microprocessor control, have measurement, setting, record, preservation and data processing function.Be provided with the RS-232/USB interface,, and be provided with functions such as help, automaticity height with the PC on-line communication.
4, chemical kit colour developing, sample and reagent dosage are few, prevent and have reduced secondary pollution.
5, large-screen lc Chinese shows, the man-machine interactive operation, and volume is little, in light weight, easy to use.
6,96 channel rapid detector for pesticide residue are by 96 light emitting diodes and 96 integrated array modularization detection systems that constitute of detecting device, no movable member.When sample detection, will get final product in the sample detection plate insertion detection module.Testing result shows on display screen at once.
A kind of array-type 96-channel rapid detector for pesticide residue sample cell of the present invention also adopts 96 hole ELISA Plate, but maintains static during sample detection, can detect 96 samples simultaneously.The detection system modular combination, no movable member, detection speed is fast, data stabilization, precision and high conformity, testing result accuracy height.The present invention can fast detecting food, organophosphorus and carbamate pesticide residue content in the vegetables, fruit, water.
Description of drawings
Fig. 1: be a kind of array-type 96-channel rapid detector for pesticide residue external structure of the present invention synoptic diagram.
Fig. 2: be 48 passage detection system modular structure synoptic diagram in a kind of array-type 96-channel rapid detector for pesticide residue of the present invention.
Fig. 3: be detection system base construction synoptic diagram in the 48 passage detection system modules in a kind of array-type 96-channel rapid detector for pesticide residue of the present invention.
Fig. 4: be detector system mainboard structure synoptic diagram in the 48 passage detection system modules in a kind of array-type 96-channel rapid detector for pesticide residue of the present invention.
Fig. 5: be light-source system mainboard structure synoptic diagram in the 48 passage detection system modules in a kind of array-type 96-channel rapid detector for pesticide residue of the present invention.
Fig. 6: be light-source system base construction synoptic diagram in the 48 passage detection system modules in a kind of array-type 96-channel rapid detector for pesticide residue of the present invention.
Fig. 7: be detector system framed structure synoptic diagram in the 48 passage detection system modules in a kind of array-type 96-channel rapid detector for pesticide residue of the present invention.
Fig. 8: be a kind of array-type 96-channel rapid detector for pesticide residue principle schematic of the present invention.
Embodiment
Below the structure of the array-type 96-channel rapid detector for pesticide residue of the present invention effect forming and can produce just, conjunction with figs. is described in detail as follows with preferred embodiment:
A kind of array-type 96-channel rapid detector for pesticide residue of the present invention is based in the sample measured matter and generates colored compound with the developer reaction behind extraction and cleaning, the instrument of a kind of colorimetric analysis that the principle that visible light 412nm is absorbed selectively according to this compound is set up.
As shown in Figure 1, the present invention has casing 1, and the front surface of casing 1 is provided with LCDs 8, button 9, and button 9 includes return key, acknowledgement key, upturning key and downturning key.Casing upper surface is provided with mini-printer 5,96 passage sample detection module cover 6, power control switch 4, supply socket 3 and RS-232 interface 2 that right side of face below, casing 1 back is provided with.Be provided with the 96 passage sample detection modules 7 that constitute by two 48 passage detection system module combinations in the casing 1.
As shown in Figure 2,48 passage detection system modules of a kind of array-type 96-channel rapid detector for pesticide residue of the present invention have a light-source system mainboard 72, one light-source system pedestal 73 is arranged, LED (light emitting diode) above the light-source system mainboard 72 and the light-source system LED bottom outlet clearance fit above the light-source system pedestal 73 above the light-source system mainboard 72.In addition, detector system mainboard 76 is placed in above the detector system pedestal 75, detecting device above the detector system mainboard 76 and the detecting device bottom outlet clearance fit above the detector system pedestal knot 75.Detector system framework 74 couples together light-source system pedestal 73 and detector system pedestal knot 75 by fastening bolt 71 in the mode of closely cooperating.
As shown in Figure 5, one light source wiring board 752 is arranged on light-source system mainboard 72, light source wiring board 752 one side distributing control circuit and light-source system mainboard web member 751, be connected with microprocessor and light-source system is sent steering order by light-source system mainboard web member 751, another side is the modularized light sources that 48 super bright light emitting diodes 753 constitute with integrated array mode.
As shown in Figure 6, light-source system pedestal knot 73 is processed into light-source system base plate 761 by aluminum alloy materials, be uniform-distribution with light-source system LED bottom outlet 762 on light-source system base plate 761, LED size and arrangement mode match in the size of light-source system LED bottom outlet 762 and arrangement mode and the light-source system wiring board.Wherein 763 is the light-source system connecting hole, and fastening bolt couples together light-source system pedestal, detector system pedestal and system's central frame by the light-source system connecting hole.
As shown in Figure 4, one detector system wiring board 742 is arranged on detector system mainboard 76, detector system wiring board 742 one side distributing control circuit and detector system mainboard web member 741, by detector system mainboard web member 741 detection signal that detecting device receives is transferred to microprocessor processes, another side is the modular array detecting device that 48 photoelectric sensors 743 constitute with integrated array mode.
As shown in Figure 3, detector system pedestal knot 75 is processed into detector system base plate 731 by aluminum alloy materials, be uniform-distribution with detector system bottom outlet 732 on detector system base plate 731, LED size and arrangement mode match in the size of detector system bottom outlet 732 and arrangement mode and the light-source system wiring board.Wherein 733 is the detector system connecting hole, and fastening bolt couples together light-source system pedestal, detector system pedestal and system's central frame by the detector system connecting hole.
As shown in Figure 7, detector system framework 74 is processed into by aluminum alloy materials, and ELISA Plate groove 773 is put in the inside of detector system frame supported frame 771, puts ELISA Plate groove 773 sizes and is cooperatively interacted by the 48 passage ELISA Plate sizes of being placed in the detector system.Wherein 772 is the detector system connecting hole, and fastening bolt couples together light-source system pedestal, detector system pedestal and system's central frame by the detector system connecting hole.
As shown in Figure 8, principle of work of the present invention is shown: microprocessor 81 is transferred to steering order in the control circuit 82 of light-source system mainboard by light-source system mainboard web member, the control circuit 82 of light-source system mainboard is according to the super bright light emitting diode of microprocessor steering order control light-source system LED, light signal is by after the ELISA Plate 83, by the photoelectric sensor on the control circuit of detector system mainboard the control circuit 84 back arrival microprocessors 81 that detected signal is transferred to the detector system mainboard are handled, testing result shows by display system 85.
In the present embodiment, adopted the Japanese super bright light emitting diode that day is inferior and Toyota Company produces, luminance parameter is more than 1000mcd.The light emitting diode of wavelength 412nm, light source adopt continuously or the pulse mode power supply.This circuit is a custom circuit, is not describing in detail at this.Photoelectric detector in the present embodiment, adopted American TI Company to produce the integrated electro sensor, model is TSL201, TSL202, TSL203, TSL204, TSL205, TSL206, TSL207, TSL208, TSL209, TSL220, TSL230, TSL235, TSL245, TSL250, TSL251, TSL250, TSL253, TSL254, TSL255, TSL256, TSL257, TSL258, TSL259, TSL260, TSL261, TSL262.Certainly, the present invention also can adopt other to have the integrated electro sensor of equal effect.Photoelectric detector arrives microprocessor 81 with detected data transmission.P51, P52 series that microprocessor 81 is produced for PHILIPS Co., its major parameter is 12 single-chip microcomputers, internal storage 64K.The output signal that microprocessor 81 is used for the receiving photoelectric detector information of line data after handling of going forward side by side is shown by LCDs 8.Simultaneously, microprocessor also can be finished by operating computer by setting, collection, analysis and the processing procedure of RS-232/USB interface and computer on-line communication data, and the information after will handling shows by display screen.Components and parts such as microprocessor of the present invention, light-source system, photoelectric detector system, LCDs 5, button 9 and power switch 4, supply socket 3 and RS-232/USB interface 2 can be connected by custom circuit.
Introduce the using method of a kind of array-type 96-channel rapid detector for pesticide residue of the present invention below in conjunction with concrete operations.
Sample preparation
Sample difference to be detected, (as rape, tomato will adopt different pre-treating methods, see the instrument operation instructions for details.) will adopt different pre-treating methods, the agricultural chemicals in the sample to be detected is extracted and is prepared into sample solution.
Colour developing-measuring operation
1. blank measure: in the ELISA Plate that certain volume distilled water is housed, add related reagent respectively, then ELISA Plate is put into 96 passage detection system modules and locked." measurement " project of selection is operated by the prompting of instrument, carries out blank sample and measures.
2. sample measurement: ELISA Plate is taken out, change the sample ELISA Plate, add related reagent, then ELISA Plate is put into 96 passage detection system modules and locked." measurement " project of selection is operated by the prompting of instrument, draws the result of sample measurement.
Though the present invention is described by previous embodiment, but still can change its form and details, make not breaking away under the spirit of the present invention.Aforementioned for the most rational using method of the present invention, one of the mode that can specifically implement for the present invention only, but not as limit.
Claims (10)
1, a kind of array-type 96-channel rapid detector for pesticide residue comprises a casing and 96 passage ELISA Plate, light source, detecting device, microprocessor and power supply located therein; It is characterized in that:
Wherein 96 passage ELISA Plate, light source and detecting device are formed the detection system module; Described light source is the array light source of 96 the integrated combination of light emitting diode, the array detecting device that described detecting device is made of 96 photoelectric sensors, described array light source and array detecting device correspondence are assembled in the framework both sides of a placement 96 passage ELISA Plate; Array light source is corresponding one by one with the position of array detecting device, and maintains static.
2, a kind of array-type 96-channel rapid detector for pesticide residue as claimed in claim 1 is characterized in that, wherein said detection system module constitutes 96 passage sample detection modules by two 48 passage detection system module combinations.
3, a kind of array-type 96-channel rapid detector for pesticide residue as claimed in claim 1 or 2, it is characterized in that, described detection system module is established a light-source system mainboard, establishes a light-source system pedestal above the light-source system mainboard, lays a plurality of light emitting diodes above the light-source system mainboard; If a detector system mainboard is placed in a detector system pedestal and ties face, establish the detecting device bottom outlet above the detector system pedestal, detecting device above the detector system mainboard and detector system pedestal are tied the detecting device bottom outlet clearance fit of face; The system framework of setting up departments in light-source system pedestal and the detector system pedestal, system framework is connected with detector system pedestal knot the light-source system pedestal by fastening bolt in the mode of closely cooperating.
4, a kind of array-type 96-channel rapid detector for pesticide residue as claimed in claim 3, it is characterized in that, described light-source system pedestal knot is established the light-source system base plate, be uniform-distribution with light-source system LED bottom outlet on the light-source system base plate, the size of light-source system LED bottom outlet and arrangement mode match with LED size and arrangement mode on the light-source system mainboard.
5, a kind of array-type 96-channel rapid detector for pesticide residue as claimed in claim 3, it is characterized in that, described detector system pedestal knot comprises a detector system base plate, be uniform-distribution with the detector system bottom outlet on the detector system base plate, LED size and arrangement mode match in the size of detector system bottom outlet and arrangement mode and the light-source system wiring board.
6, a kind of array-type 96-channel rapid detector for pesticide residue as claimed in claim 3 is characterized in that, the inside of described system framework is provided with the dead slot of putting ELISA Plate, and described dead slot size matches with described ELISA Plate size.
7, a kind of array-type 96-channel rapid detector for pesticide residue as claimed in claim 3, it is characterized in that, wherein on the detector system base plate, establish the detector system connecting hole, on the light-source system base plate, establish the light-source system connecting hole, light-source system pedestal, detector system pedestal and system framework are coupled together by detector system connecting hole and light-source system connecting hole.
8, as claim 1 or 2 or 4 to 7 arbitrary described a kind of array-type 96-channel rapid detector for pesticide residue, it is characterized in that, by the super bright light emitting diode combined forming array formula light source of single wavelength 412nm.
9, as claim 1 or 2 or 4 to 7 arbitrary described a kind of array-type 96-channel rapid detector for pesticide residue, it is characterized in that, described casing front panel is provided with LCDs and a plurality of button, described casing upper surface is provided with printer interface, 96 passage sample detection module cover, and described casing rear portion also is provided with supply socket, power switch and RS232/USB interface.
10, a kind of array-type 96-channel rapid detector for pesticide residue as claimed in claim 8, it is characterized in that, described casing front panel is provided with LCDs and a plurality of button, described casing upper surface is provided with printer interface, 96 passage sample detection module cover, and described casing rear portion also is provided with supply socket, power switch and RS232/USB interface.
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Cited By (9)
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| CN102645405A (en) * | 2012-03-29 | 2012-08-22 | 中国农业大学 | Rotary positioning device and detection system for detecting pesticide residue on surface of fruits |
| CN103558258A (en) * | 2013-11-19 | 2014-02-05 | 山东理工大学 | Antibiotic multiresidue detector based on array sensors |
| CN103558257A (en) * | 2013-11-19 | 2014-02-05 | 山东理工大学 | Pesticide multiresidue detector based on array sensors |
| CN104181112A (en) * | 2013-05-21 | 2014-12-03 | 浙江托普仪器有限公司 | Sixteen-channel pesticide residue rapid detector |
| CN105158373A (en) * | 2015-09-18 | 2015-12-16 | 山东省农业科学院农业质量标准与检测技术研究所 | Rapid detection and analysis device for organic pollutants in agricultural products |
| CN105866355A (en) * | 2016-04-14 | 2016-08-17 | 山东省农业科学院农业质量标准与检测技术研究所 | Multi-residual-pesticide multi-direction detector capable of wirelessly transmitting data |
| CN107101945A (en) * | 2017-05-26 | 2017-08-29 | 北京智云达科技股份有限公司 | A kind of multichannel multi-wavelength light spectrum detection device |
| CN108398394A (en) * | 2018-04-24 | 2018-08-14 | 武汉中科志康生物科技有限公司 | Full-automatic instrument for detecting pesticide residue through |
| CN108645792A (en) * | 2018-04-26 | 2018-10-12 | 北京勤邦生物技术有限公司 | A kind of rapid detector for pesticide residue |
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| EP2420824B1 (en) * | 2001-06-29 | 2018-11-28 | Meso Scale Technologies LLC | Multi-well plate having an array of wells and kit for use in the conduct of an ECL assay |
| CN2611904Y (en) * | 2003-03-11 | 2004-04-14 | 吉林大学化学学院 | Multifunctional detector for poisonous chemical residual pollutant in foodstuff |
| CN201166659Y (en) * | 2008-03-20 | 2008-12-17 | 长春吉大·小天鹅仪器有限公司 | Device for rapidly detecting array type 96chunnel pesticide residue |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102645405A (en) * | 2012-03-29 | 2012-08-22 | 中国农业大学 | Rotary positioning device and detection system for detecting pesticide residue on surface of fruits |
| CN102645405B (en) * | 2012-03-29 | 2014-03-05 | 中国农业大学 | Rotary positioning device and detection system for detecting pesticide residue on surface of fruits |
| CN104181112A (en) * | 2013-05-21 | 2014-12-03 | 浙江托普仪器有限公司 | Sixteen-channel pesticide residue rapid detector |
| CN103558258A (en) * | 2013-11-19 | 2014-02-05 | 山东理工大学 | Antibiotic multiresidue detector based on array sensors |
| CN103558257A (en) * | 2013-11-19 | 2014-02-05 | 山东理工大学 | Pesticide multiresidue detector based on array sensors |
| CN103558257B (en) * | 2013-11-19 | 2015-05-13 | 山东理工大学 | Pesticide multiresidue detector based on array sensors |
| CN105158373A (en) * | 2015-09-18 | 2015-12-16 | 山东省农业科学院农业质量标准与检测技术研究所 | Rapid detection and analysis device for organic pollutants in agricultural products |
| CN105158373B (en) * | 2015-09-18 | 2019-02-12 | 山东省农业科学院农业质量标准与检测技术研究所 | Organic pollutant quickly detects and analytical equipment in a kind of agricultural product |
| CN105866355A (en) * | 2016-04-14 | 2016-08-17 | 山东省农业科学院农业质量标准与检测技术研究所 | Multi-residual-pesticide multi-direction detector capable of wirelessly transmitting data |
| CN105866355B (en) * | 2016-04-14 | 2018-02-13 | 山东省农业科学院农业质量标准与检测技术研究所 | It is a kind of can Wireless data transmission data the multi-direction detector of multi-tesidual pesticides |
| CN107101945A (en) * | 2017-05-26 | 2017-08-29 | 北京智云达科技股份有限公司 | A kind of multichannel multi-wavelength light spectrum detection device |
| CN108398394A (en) * | 2018-04-24 | 2018-08-14 | 武汉中科志康生物科技有限公司 | Full-automatic instrument for detecting pesticide residue through |
| CN108645792A (en) * | 2018-04-26 | 2018-10-12 | 北京勤邦生物技术有限公司 | A kind of rapid detector for pesticide residue |
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