CN101538525B - Fermentation process adopting material outside circulation and equipment thereof - Google Patents
Fermentation process adopting material outside circulation and equipment thereof Download PDFInfo
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- CN101538525B CN101538525B CN 200910020432 CN200910020432A CN101538525B CN 101538525 B CN101538525 B CN 101538525B CN 200910020432 CN200910020432 CN 200910020432 CN 200910020432 A CN200910020432 A CN 200910020432A CN 101538525 B CN101538525 B CN 101538525B
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- fermentor tank
- injector
- tank
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
Abstract
The invention relates to an aerobic fermentation process and equipment, in particular to a brand new aerobic fermentation process adopting material jar outside circulation and equipment thereof. The process adopts the mode that materials circulate outside a jar so as to achieve the aim of sufficiently mixing air and material liquid, thereby improving the efficiency of fermentation, reducing the energy consumption required by the whole process and improving the production efficiency of enterprises.
Description
Technical field
The present invention relates to a kind of zymotechnique, particularly a kind of aerobic fermentation process and equipment thereof that adopts the material pot outer circulation.
Background technology
In the relevant industries of fermentation, aerobic fermentation is the most general a kind of, aerobic fermentation refers to that zymophyte finishes the fermenting process to material in being rich in the environment of oxygen, traditional zymotechnique is: the mass transport after process liquefaction, the filtration is to fermentor tank, be not higher than sterilising treatment under 130 ℃ the temperature, the Multi-stage stirring that blowing air utilizes fermentor tank after inoculation carries out the mixing fermentation with material and air, and this fermentation process is widely used at fermentation industry, as the industry such as brewages.
In the existing zymotechnique, air is the main source of oxygen, and it is injected in the fermentor tank by injector, drives fermented liquid to finish whole fermenting process, and what in fact adopt is the technique that extraneous air drives materials inside.General injector is installed in injection fermentation materials in the tank, but because the relative material of kinetic energy of air is very little, thereby the injection effect that produces is faint, can't drive the material in the whole tank, portion nozzle can not form jet like this, the mixed effect of air and material is poor, the low ferment effect of the oxygen level of material is not obvious, for addressing this problem, present common practice is to adopt churned mechanically method, impels the further mixing of gas-liquid, but drive the mechanical energy that the interior material of whole tank still must be stronger, thereby cause the greatly rising of energy consumption, for whole enterprise, it is not remarkable to produce energy-conservation effect.In the improvement of existing fermentor tank and zymotechnique, once there was enterprise to strengthen the jet power of injector in the hope of reaching better mixed effect, but so just caused fermented bacterium to be destroyed, whole ferment effect does not rise counter falling, and energy consumption does not descend yet.
Summary of the invention
Many weak points for existing zymotechnique existence, the invention provides a kind of brand-new aerobic fermentation process and equipment, it adopts material in the mode of tank outer circulation to reach the well-mixed purpose of air and feed liquid, improved the efficient of fermentation, reduced the required energy consumption of whole technique and improved the production efficiency of enterprise.
Zymotechnique provided by the present invention, its concrete grammar is: outside feeding pipe was sent into fermentor tank, by sending in the injector after the recycle pump pressurization, material and air mixed in injector after the pressurization by injector, sent back in the fermentor tank with material.
For the effect that makes fermentation is improved, can the interior leavening temperature of controlled fermentation tank be 20 ℃-50 ℃, the constant temperature sterilising temp of the earlier fermentation of fermentor tank is 60 ℃-130 ℃, and sterilization time is 2-60min, and the volume of fermentor tank is at 50-1000m
3, the inventory in the fermentor tank is the 70%-95% of cubic capacity.Carry out the constant temperature sterilization in the early stage of fermentation, temperature is 60 ℃-130 ℃, can guarantee that like this normal growth of flora in the process of fermentation is removed other pernicious bacterias simultaneously for the impact of fermentation.The volume of fermentor tank is different according to different fermentations technique, but generally all is controlled at 50-1000m
3Inventory is controlled to be the 70%-95% of fermentor tank cubic capacity, can guarantee like this effect of fermenting, the load that prevents fermentor tank is excessive, cause equipment normally to move, the moment increase that volume can not occur the material in the time of can guaranteeing material outside circulation simultaneously in the fermentor tank causes fermentor tank impaired.This circulation device begins with the unlatching of zymotechnique, carry out synchronously with reaction, to realize in the whole reaction needs for oxygen, simultaneously also can guarantee after the zymotechnique autoreaction begins a metastable reaction environment is just arranged, can owing to unexpected ON cycle device causes reaction environment destroyed, not affect the yield of fermentation reaction.
Zymotechnique for this tank outer circulation, the invention provides the fermentation equipment that adapts with it, this equipment comprises fermentor tank, in the fermentor tank or fermentor tank at least one injector arranged outward, also injector can be set all outside the tank inner canister, the fluid inlet of injector is connected by the outer circulating-pump outlet of infusion pipeline and tank, the fluid inlet of recycle pump is connected with fermentor tank by at least one mass transport pipeline, the airline import of injector communicates with atmosphere by sterilization filter device or is communicated with fan outlet, the liquid outlet of injector is positioned at fermentor tank, also can be arranged on outside the fermentor tank, for the better sterility that improves the efficient of fermentation and guarantee fermenting process, generally it is arranged in the fermentor tank; Injector quantity is at 1-200, and injector is made by the principle of Venturi tube.
After adopting Processes and apparatus of the present invention, at first in fermentor tank, put in use material to the 70%-95% of cubic capacity, with heating material to 60 ℃-130 ℃ of sterilization 2-60min, be cooled to 20 ℃-50 ℃, inoculation, ventilate, can opening outer circulation pump fermentation beginning, regulate the related process parameter of recycle pump and injector to adapt to different fermentations technique for the requirement of flow of oxygen, this moment, material was sent into injector after pressurizeing through the outer circulation pump, in the nozzle of injector air with the pressurization after feed liquid contact, air is crushed into tiny bubble and sneaks into uniformly in the feed liquid, thereby finish the mixing of gas-liquid, feed liquid afterwards sends back in the fermentor tank by the liquid outlet of injector, and material is finished the whole process of fermentation in tank.Jet apparatus is according to the difference of fermentor tank volume in whole process, air capacity is controlled to be 1-8 times/hour of tank body cubic capacity, the pressure of air is regulated between 0.1MPa-0.55MPa, internal circulating load is 5-10 times/hour of fermentor tank cubic capacity, difference according to the fermentation time section, recycle pump circulates to material by the endless tube different orientation from tank body that distributes in the tank body, the recycle pump that adopts is propeller pump or mixedflow pump or impeller pump, and the device of kinetic energy perhaps can be provided the outer circulation of material with other forms.
Because various zymotechniques are different for the requirement of fermentation condition, so injector can be arranged in the fermentor tank and also can be arranged on outside the fermentor tank; Can reach the purpose of balanced gas-liquid for the effect that makes injection, generally the injector quantity of installing inside and outside the tank is controlled at 1-200, specifically looking the power of injector and the requirement of technique determines, if but injector quantity too much can increase the energy consumption of whole technique greatly, therefore it is limited in 200.
In order to reach better gas-liquid mixed effect, the whipping device of at least one cover also is installed in fermentor tank, like this feed liquid in injector is sent into tank under the effect of agitator again with tank in original feed liquid mix, be exactly to have reached the maximum mixing of gas-liquid in the whole tank like this, for fermentation provides best processing condition.
The used injector of the present invention is the jet type injecting device, this injector is by nozzle, trunnion or nozzle, trunnion, mixing section, expansion section forms, injector designs becomes the form with liquid ejecting gas, specifically can adopt buy on the market with the liquid ejecting insufflator, the volume ratio of liquid and gas is 1: 0.2-1: 10, injector level or vertical the placement, interlock in the form of a ring or the uniform arrangement of ring-type, adopt this placement and spread pattern, can increase the interior volume of material of tank that injection affects, thereby reach better gas-liquid Blending Efficiency of Blending, help material better to ferment, with the volume ratio of liquid and gas control again 1: 0.2-1: 10, can make so all to contain the air capacity that band is fit in the feed liquid of unit volume, can not cause Efficiency Decreasing in the fermenting process and the unbalanced branch of oxygen level, this ratio is a value range simultaneously, can concomitant fermentations reaction carrying out and the fact is adjusted, to satisfy the differential responses period for the consumption of oxygen.The airline import of injector has sterilization filter device, can prevent like this external environment (such as airborne bacterium, suspended substance etc.) for the disadvantageous effect of environment in the fermentation, guarantees the stable and safety of fermented bacterium.
Rely on kinetic energy that recycle pump produces liquid through the nozzle ejection gas, liquid and gas mix rapidly the abundant contact process of finishing material and air in nozzle.Fermented bacterium is relatively fragile; yeasting in the fermentor tank is not allowed to pollute; and present fermentation spraying technique adopts powerful internal recycling; so just certainly will cause the destruction to bacterial classification; therefore in technique of the present invention, adopted the pressure outer loop of recycle pump; the sealing property of recycle pump better can not damage the environment in the tank; and the outer loop of feed liquid has also increased the protection for the zymophyte bacterial classification; this technology is that the material after the sterilising treatment is carried out pump circulation simultaneously, and the provide protection of yet having played for fermented bacterium of growing of other bacterial classifications can not occur in the process of outer circulation like this.
Adopt zymotechnique and the equipment thereof of material pot outer circulation provided by the present invention, changed traditional injector with the spray regime of band of gas material, and adopt the outer pump circulation of fermentor tank to increase kinetic energy to material, then with strip gas.Rely on the powerful kinetic energy of material that air ejection is entered fermentor tank and in nozzle with the air collision, tear into bubble in small, broken bits, the purpose that fully contacts to reach material and air.So just increased the contact area between gas-liquid, more effectively mixing material and air are conducive to zymogenic growth; Simultaneously owing to contain high kinetic energy in the material of spurting into, around injector, can form vortex, thereby reach the purpose that stirs material in the whole tank, reduced the operating pressure of big machinery whipping device, reduced and produced required energy consumption, whole watt consumption is than traditional zymotic tank decline 10-50%; Guaranteed stopping property and the independence of fermenting process, be fit to various aerobic fermentation process, with regard to whole zymotechnique, the fermentation dissolved oxygen is 2-5 times of prior art, and fermentation period reduces 3-10h on year-on-year basis, has reduced the production cost of enterprise, has created benefit.This technology can be applied to all fermentative production industries relevant with aerobic fermentation, can assist with mechanical stirring to eliminate dead angle in the tank, fermentation materials is fully contacted with air, reach and save stirring kinetic energy, improve dissolved oxygen, and shorten the purpose of fermentation period, raising fermentation index.
Description of drawings
Fig. 1 is the process flow sheet of zymotechnique of the present invention;
Fig. 2 is the structural representation of fermentation equipment in the apparatus embodiments 1 of the present invention
1 recycle pump among the figure, 2 injectors, 3 whipping apptss, 4 and 5 feeding pipes, 6 recycle pump charging lines, 7 injector airflow pipes, 8 fermentor tanks.
Embodiment
Process implementing example 1
At 500m
3Fermentor tank in put material to 90% of cubic capacity, with heating material to 100 ℃ sterilization 30min, be cooled to 20 ℃-40 ℃, inoculation fermentation bacterium, ventilation, afterwards ON cycle pump fermentation beginning, the flow of controlled circulation pump is 2500m
3/ h-4000m
3/ h, the air capacity that injector sucks is from 1000m
3/ h-4000m
3Regulate between/h, air pressure is 0.2MPa-0.35MPa, sends back in the fermentor tank through the injected device of feed liquid of injector mixing air.
After adopting this zymotechnique, the fermentation dissolved oxygen content is 4 times of former zymotechnique, and fermentation period reduces 10h on year-on-year basis, and watt consumption descends 40% than the traditional zymotic tank.
Process implementing example 2
At 50m
3Fermentor tank in put material to 75% of cubic capacity, with heating material to 110 ℃ sterilization 60min, be cooled to 30 ℃-50 ℃, inoculation fermentation bacterium, ventilation, afterwards ON cycle pump fermentation beginning, the flow of controlled circulation pump is 250m
3/ h-500m
3/ h, the air capacity that injector sucks is from 200m
3/ h-400m
3Regulate between/h, air pressure is 0.2MPa-0.50MPa, sends back in the fermentor tank through the injected device of feed liquid of injector mixing air.
After adopting this technique, the fermentation dissolved oxygen content is 2.5 times of former zymotechnique, and fermentation period reduces 3h on year-on-year basis, and watt consumption descends 25% than the traditional zymotic tank.
Process implementing example 3
At 1000m
3Fermentor tank in put material to 80% of cubic capacity, with heating material to 70 ℃ sterilization 40min, be cooled to 35 ℃-50 ℃, inoculation fermentation bacterium, ventilation, afterwards ON cycle pump fermentation beginning, the flow of controlled circulation pump is 5000m
3/ h-8000m
3/ h, the air capacity that injector sucks is from 4000m
3/ h-7500m
3Regulate between/h, air pressure is 0.35MPa-0.45MPa, sends back in the fermentor tank through the injected device of feed liquid of injector mixing air.
After adopting this technique, the fermentation dissolved oxygen content is 5 times of former zymotechnique, and fermentation period reduces 8h on year-on-year basis, and watt consumption descends 55% than the traditional zymotic tank.
Process implementing example 4
At 250m
3Fermentor tank in put material to 90% of cubic capacity, with heating material to 120 ℃ sterilization 5min, be cooled to 20 ℃-40 ℃, inoculation fermentation bacterium, ventilation, afterwards ON cycle pump fermentation beginning, the flow of controlled circulation pump is 1000m
3/ h-2000m
3/ h, the air capacity that injector sucks is from 500m
3/ h-1500m
3Regulate between/h, air pressure is 0.15MPa-0.30MPa, sends back in the fermentor tank through the injected device of feed liquid of injector mixing air.
After adopting this zymotechnique, the fermentation dissolved oxygen content is 3.5 times of former zymotechnique, and fermentation period reduces 5h on year-on-year basis, and watt consumption descends 45% than the traditional zymotic tank.
Apparatus embodiments 1
A kind of aerobic fermentation equipment that adopts the material pot outer circulation, comprise fermentor tank 8,20 injectors are arranged in the fermentor tank 8, the fluid inlet of injector 2 is connected by outer recycle pump 1 outlet of recycle pump charging line 6 and tank, the fluid inlet of recycle pump 1 is connected with fermentor tank 8 with being connected by mass transport pipeline 4, the airline import 7 of injector communicates with atmosphere by sterilization filter device or is communicated with fan outlet, and the liquid outlet of injector is positioned at fermentor tank.
One-level whipping device 3 is installed in the fermentor tank 8, and whipped form is arrow leaf formula, and the angle of paddle is being 35 °.If do not adopt stirring, the effect of fermentation can descend to some extent, adopts the angle of paddle when being 35 °, and its energy consumption is minimum simultaneously for the best results of stirring.
The injector level is installed in the fermentor tank, and is staggered in the form of a ring in fermentor tank, and this injector is comprised of nozzle, trunnion or nozzle, trunnion, mixing section, expansion section, and its structure is consistent with existing injector.
Apparatus embodiments 2
A kind of aerobic fermentation equipment that adopts the material pot outer circulation, comprise fermentor tank, fermentor tank has 200 injectors outward, the fluid inlet of injector is connected by the outer circulating-pump outlet of recycle pump charging line and tank, the fluid inlet of recycle pump is connected with the bottom of fermentor tank by the mass transport pipeline, the airline import of injector is communicated with fan outlet, and the liquid outlet of injector is positioned at fermentor tank.
The injector level is installed in outside the fermentor tank, uniform in the form of a ring arrangement outside fermentor tank, and this injector adopts the injector commonly used on the market.
A kind of aerobic fermentation equipment that adopts the material pot outer circulation, comprise fermentor tank, be separately installed with 20 and 50 injectors outward with fermentor tank in the fermentor tank, the fluid inlet of injector is connected by the outer circulating-pump outlet of recycle pump charging line and tank, the fluid inlet of recycle pump is connected with top, middle part and the bottom of fermentor tank respectively by the mass transport pipeline, the airline import of injector communicates with atmosphere by sterilization filter device, and the liquid outlet of injector all is positioned at fermentor tank.
3 grades of whipping devices 3 are installed in the fermentor tank 8, and whipped form is arrow leaf formula, and the angle of paddle is respectively 12 °, and 35 °, 40 °.Whipping device in the present embodiment must arrange, and adopts above-mentioned paddle angle, and such differential seat angle can effectively be controlled speed and the direction of stirring, can improve final ferment effect and reduce energy consumption.
Injector is horizontal positioned in fermentor tank, and vertical the placement is staggered in the form of a ring in fermentor tank outside fermentor tank, uniform in the form of a ring arrangement outside tank, and above-mentioned injector all adopts liquid ejector commonly used.
Claims (1)
1. zymotechnique that adopts material outside circulation, it is characterized in that: concrete grammar is: with material outside feeding pipe is sent into fermentor tank, by sending in the jet apparatus after the circulation device pressurization, in jet apparatus, the material after the pressurization and air mixed also send back in the fermentor tank by injector and feeding pipeline;
The feed liquid internal circulating load of wherein said circulation device is 5-10 times/hour of fermentor tank cubic capacity;
The air capacity of described jet apparatus is controlled to be 1-8 times/hour of tank body cubic capacity, air pressure 0.1MPa-0.55Mpa;
Described jet apparatus is with liquid ejecting gas, and the volume ratio of liquid and gas is 1: 0.2-1: 10;
The leavening temperature of described technique is 20 ℃-50 ℃, and ferment tank constant temperature sterilising temp in earlier stage is 60 ℃-130 ℃, and sterilization time is 2-60min, and the volume of fermentor tank is at 50-1000m
3, the inventory in the fermentor tank is the 70%-95% of cubic capacity;
Realize the fermentation equipment of this technique, comprise fermentor tank, in the fermentor tank and/or fermentor tank at least one injector arranged outward, the fluid inlet of injector is connected by the outer circulating-pump outlet of infusion pipeline and tank, the fluid inlet of recycle pump is connected with fermentor tank by at least one mass transport pipeline, and the liquid outlet of injector is in fermentor tank; Described ejector quantity is at 1-200, and injector level or vertical the placement are interlocked or the uniform arrangement of ring-type in the form of a ring; The whipping device of at least one cover also is installed in the described fermentor tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910020432 CN101538525B (en) | 2009-04-08 | 2009-04-08 | Fermentation process adopting material outside circulation and equipment thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910020432 CN101538525B (en) | 2009-04-08 | 2009-04-08 | Fermentation process adopting material outside circulation and equipment thereof |
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| Publication Number | Publication Date |
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| CN101538525A CN101538525A (en) | 2009-09-23 |
| CN101538525B true CN101538525B (en) | 2013-04-17 |
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| CN 200910020432 Expired - Fee Related CN101538525B (en) | 2009-04-08 | 2009-04-08 | Fermentation process adopting material outside circulation and equipment thereof |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102876567B (en) * | 2012-10-30 | 2014-06-25 | 南京通用电气装备有限公司 | Biomass anaerobic fermentation tank stirring and heating method and biomass anaerobic fermentation tank stirring and heating system |
| CN106754296B (en) * | 2016-12-09 | 2019-06-21 | 昆山博青生物科技有限公司 | The installation for fermenting and method of high-efficiency fermenting production bacteriocin |
| CN107616489A (en) * | 2017-08-01 | 2018-01-23 | 张萌 | A kind of ferment fermentation system |
| CN112939656A (en) * | 2021-02-26 | 2021-06-11 | 鹤壁东海生物科技有限公司 | Automatic control-based flow type aerobic fermentation tank and aerobic fermentation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87213094U (en) * | 1987-09-02 | 1988-05-25 | 华南工学院 | Pulse overflow self-sucking type sprayer |
| CN1032190A (en) * | 1987-09-04 | 1989-04-05 | 丹麦生物蛋白有限公司 | Produce the method and apparatus of microorganism cells body |
| CN2272782Y (en) * | 1996-09-06 | 1998-01-21 | 中国食品发酵工业研究所 | Jet multistage gas-liquid mixing apparatus |
-
2009
- 2009-04-08 CN CN 200910020432 patent/CN101538525B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87213094U (en) * | 1987-09-02 | 1988-05-25 | 华南工学院 | Pulse overflow self-sucking type sprayer |
| CN1032190A (en) * | 1987-09-04 | 1989-04-05 | 丹麦生物蛋白有限公司 | Produce the method and apparatus of microorganism cells body |
| CN2272782Y (en) * | 1996-09-06 | 1998-01-21 | 中国食品发酵工业研究所 | Jet multistage gas-liquid mixing apparatus |
Non-Patent Citations (2)
| Title |
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| 徐清华等.机械搅拌通风发酵罐的节能设计.《食品与发酵工业》.2009,(第3期),134-136. * |
| 郑裕国.外循环气升式发酵罐液相循环时间及其应用于α_淀粉酶发酵的研究.《中国食品学报》.1998,第2卷(第1期),17-21. * |
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