CN101526476B - 确定聚合酶链式反应过程数据是否显示显著生长的方法以及进行所述方法的装置和pcr系统 - Google Patents

确定聚合酶链式反应过程数据是否显示显著生长的方法以及进行所述方法的装置和pcr系统 Download PDF

Info

Publication number
CN101526476B
CN101526476B CN2008101497410A CN200810149741A CN101526476B CN 101526476 B CN101526476 B CN 101526476B CN 2008101497410 A CN2008101497410 A CN 2008101497410A CN 200810149741 A CN200810149741 A CN 200810149741A CN 101526476 B CN101526476 B CN 101526476B
Authority
CN
China
Prior art keywords
curve
pcr
value
data set
data
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN2008101497410A
Other languages
English (en)
Chinese (zh)
Other versions
CN101526476A (zh
Inventor
A·萨内
R·T·库尼克
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN101526476A publication Critical patent/CN101526476A/zh
Application granted granted Critical
Publication of CN101526476B publication Critical patent/CN101526476B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
    • G06F17/10—Complex mathematical operations
    • G06F17/18—Complex mathematical operations for evaluating statistical data, e.g. average values, frequency distributions, probability functions, regression analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Data Mining & Analysis (AREA)
  • Computational Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mathematical Analysis (AREA)
  • Mathematical Optimization (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pure & Applied Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mathematical Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Theoretical Computer Science (AREA)
  • Databases & Information Systems (AREA)
  • Molecular Biology (AREA)
  • Software Systems (AREA)
  • Evolutionary Biology (AREA)
  • Algebra (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Probability & Statistics with Applications (AREA)
  • Operations Research (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Image Analysis (AREA)
CN2008101497410A 2007-09-25 2008-09-25 确定聚合酶链式反应过程数据是否显示显著生长的方法以及进行所述方法的装置和pcr系统 Active CN101526476B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/861,188 US20090119020A1 (en) 2007-09-25 2007-09-25 Pcr elbow determination using quadratic test for curvature analysis of a double sigmoid
US11/861188 2007-09-25
US11/861,188 2007-09-25

Publications (2)

Publication Number Publication Date
CN101526476A CN101526476A (zh) 2009-09-09
CN101526476B true CN101526476B (zh) 2013-11-13

Family

ID=40475156

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2008101497410A Active CN101526476B (zh) 2007-09-25 2008-09-25 确定聚合酶链式反应过程数据是否显示显著生长的方法以及进行所述方法的装置和pcr系统

Country Status (5)

Country Link
US (1) US20090119020A1 (https=)
EP (1) EP2107470B1 (https=)
JP (1) JP6009724B2 (https=)
CN (1) CN101526476B (https=)
CA (1) CA2639714A1 (https=)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8374795B2 (en) 2008-05-13 2013-02-12 Roche Molecular Systems, Inc. Systems and methods for step discontinuity removal in real-time PCR fluorescence data
US8219366B2 (en) * 2009-08-26 2012-07-10 Roche Molecular Sytems, Inc. Determination of elbow values for PCR for parabolic shaped curves
EP2475987B1 (en) * 2009-09-12 2021-12-15 Azure Vault Ltd Identifying transition points in chemical reactions
WO2011061568A1 (en) 2009-11-22 2011-05-26 Azure Vault Ltd. Automatic chemical assay classification
US8666673B2 (en) 2010-05-14 2014-03-04 Biomerieux, Inc Identification and/or characterization of a microbial agent using taxonomic hierarchical classification
US9988675B2 (en) 2011-03-15 2018-06-05 Azure Vault Ltd. Rate based identification of reaction points
US8738303B2 (en) * 2011-05-02 2014-05-27 Azure Vault Ltd. Identifying outliers among chemical assays
US8660968B2 (en) 2011-05-25 2014-02-25 Azure Vault Ltd. Remote chemical assay classification
US20130096845A1 (en) 2011-10-14 2013-04-18 Ze'ev Russak Cumulative differential chemical assay identification
US10475527B2 (en) 2012-03-22 2019-11-12 Biomerieux, Inc. Method and system for detection of microbial growth in a specimen container
US10176293B2 (en) 2012-10-02 2019-01-08 Roche Molecular Systems, Inc. Universal method to determine real-time PCR cycle threshold values
US9607128B2 (en) 2013-12-30 2017-03-28 Roche Molecular Systems, Inc. Detection and correction of jumps in real-time PCR signals
US10519759B2 (en) 2014-04-24 2019-12-31 Conocophillips Company Growth functions for modeling oil production
JP7493300B2 (ja) * 2017-06-27 2024-05-31 東洋紡株式会社 解析用プログラムおよび解析装置
JP7012151B2 (ja) 2017-09-28 2022-01-27 シージーン アイエヌシー 試料内ターゲット分析物を分析する方法及び装置
DE102020202363A1 (de) * 2020-02-25 2021-08-26 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens
CN115312123B (zh) * 2021-05-07 2026-01-23 台达电子工业股份有限公司 数据预筛方法及其数据预筛装置
US20240418649A1 (en) 2021-10-21 2024-12-19 Seegene, Inc. Device and method for reading positive/negative with respect to target assay substance in sample
CN114339768B (zh) * 2021-12-24 2024-11-22 观源(上海)科技有限公司 一种usim卡抗侧信道攻击能力评估方法及其系统
US20250336479A1 (en) 2022-07-29 2025-10-30 Seegene, Inc Apparatus and method for providing interface for setting threshold value of parameter
WO2024128878A1 (ko) 2022-12-15 2024-06-20 주식회사 씨젠 형광 데이터의 분석 알고리즘에 대한 성능 비교 결과를 디스플레이하는 장치 및 방법
CN116473512B (zh) * 2023-03-22 2024-05-03 上海交通大学 一种动物循环系统中外泌体的监测装置及监测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1987880A (zh) * 2005-12-20 2007-06-27 霍夫曼-拉罗奇有限公司 利用双s形函数曲线拟合和levenberg－marquardt算法及标准化的pcr肘确定

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
ES2253778T3 (es) 1996-06-04 2006-06-01 University Of Utah Research Foundation Sistema y metodos de monitorizacion de una amplificacion de adn mediante fluorescencia.
US7228237B2 (en) * 2002-02-07 2007-06-05 Applera Corporation Automatic threshold setting and baseline determination for real-time PCR
EP1335028B1 (en) * 2002-02-12 2008-01-16 University of Utah Research Foundation Multi-test analysis of real-time nucleic acid amplifications
US20060009916A1 (en) * 2004-07-06 2006-01-12 Xitong Li Quantitative PCR data analysis system (QDAS)
CA2603389C (en) * 2005-05-13 2012-07-10 Bio-Rad Laboratories, Inc. Identifying statistically linear data
EP3168766B1 (en) * 2005-09-20 2023-08-16 University of Utah Research Foundation Genotyping method for a plurality of samples
EP1798650A1 (en) * 2005-12-19 2007-06-20 Roche Diagnostics GmbH Analytical method and instrument
US7844403B2 (en) * 2005-12-20 2010-11-30 Roche Molecular Systems, Inc. Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression
US7680868B2 (en) * 2005-12-20 2010-03-16 Roche Molecular Systems, Inc. PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization
EP1804172B1 (en) * 2005-12-20 2021-08-11 Roche Diagnostics GmbH PCR elbow determination using curvature analysis of a double sigmoid

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1987880A (zh) * 2005-12-20 2007-06-27 霍夫曼-拉罗奇有限公司 利用双s形函数曲线拟合和levenberg－marquardt算法及标准化的pcr肘确定

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Jochen Wilhelm,et al..SoFAR:Software for Fullly Automatic Evaluation of Real-Time PCR Data.《BioTechniques》.2003,第34卷324-332.
Rosalia Aguirre－Hernandez,et al..Computational RNA secondary structure design: empirical complexity and improved methods.《BMC Bioinformatics》.2007,第34卷(第8期),1-16. *
SoFAR:Software for Fullly Automatic Evaluation of Real-Time PCR Data;Jochen Wilhelm,et al.;《BioTechniques》;20030228;第34卷;324-332 *

Also Published As

Publication number Publication date
EP2107470A3 (en) 2017-09-06
CA2639714A1 (en) 2009-03-25
CN101526476A (zh) 2009-09-09
JP6009724B2 (ja) 2016-10-19
EP2107470B1 (en) 2023-08-30
JP2009080811A (ja) 2009-04-16
HK1133697A1 (en) 2010-04-01
EP2107470A2 (en) 2009-10-07
US20090119020A1 (en) 2009-05-07

Similar Documents

Publication Publication Date Title
CN101526476B (zh) 确定聚合酶链式反应过程数据是否显示显著生长的方法以及进行所述方法的装置和pcr系统
EP1801706B1 (en) PCR elbow determination by use of a double sigmoid function curve fit with the Levenberg-Marquardt algorithm and normalization
CN1987880B (zh) 利用双s形函数曲线拟合和levenberg-marquardt算法及标准化的pcr肘确定
CN101710363B (zh) 通过无方程算法判定实时pcr拐点
CN101587517B (zh) 用于除去实时pcr荧光数据中的步长不连续的系统和方法
EP1804172B1 (en) PCR elbow determination using curvature analysis of a double sigmoid
US7991562B2 (en) PCR elbow determination using curvature analysis of a double sigmoid
EP1801237B1 (en) Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression
HK1133697B (en) A method for determining whether data for a pcr process exhibits significant growth, an apparatus and a pcr system to carry out the method
HK1106304A (en) Pcr elbow determination using curvature analysis of a double sigmoid
HK1106305A (en) Temperature step correction with double sigmoid levenberg-marquardt and robust linear regression
HK1105689B (en) Pcr elbow determination by use a double sigmoid function curve fit with the levenberg-marquardt and normalization
HK1139214B (en) Systems and methods for step discontinuity removal in real-time pcr fluorescence data

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1133697

Country of ref document: HK

C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1133697

Country of ref document: HK