CN101513438A - Method for extracting panax notoginseng saponins from fresh panax notoginsengs - Google Patents
Method for extracting panax notoginseng saponins from fresh panax notoginsengs Download PDFInfo
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- CN101513438A CN101513438A CNA2008100581146A CN200810058114A CN101513438A CN 101513438 A CN101513438 A CN 101513438A CN A2008100581146 A CNA2008100581146 A CN A2008100581146A CN 200810058114 A CN200810058114 A CN 200810058114A CN 101513438 A CN101513438 A CN 101513438A
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Abstract
The invention relates to a method for extracting panax notoginseng saponins from fresh panax notoginsengs, which comprises the following steps: adopting the fresh panax notoginsengs as raw materials; crushing the panax notoginsengs; soaking the panax notoginsengs in ethanol and extracting the same for three times; recycling the ethanol from an extracting solution; adsorbing a concentrated solution with D101 macroporous adsorbent resin; washing off impurities first with water, and then performing elution with the ethanol; collecting an eluent and recycling the ethanol; and passing the concentrated solution through an alumina column. With the method, the extraction ratio of the panax notoginseng saponins can reach more than 14 percent, which is at least 2 percentage points higher than other extraction methods.
Description
Technical field:
A kind of method of extracting Radix Notoginseng total arasaponins from bright Radix Notoginseng belongs to the field of Chinese medicines.
Background technology:
Radix Notoginseng total arasaponins is the effective constituent that mainly contains in the Radix Notoginseng, be widely used in treating cardiovascular and cerebrovascular vessel, present Radix Notoginseng total arasaponins extraction process mainly is to adopt organic solvent extractionprocess, and raw material selects for use dry Radix Notoginseng to extract, Radix Notoginseng total arasaponins loses in dry run, causes shortcomings such as this extracting method extraction ratio is low, organic solvent residual, complicated operation.
Summary of the invention:
The objective of the invention is to deficiency at existing extracting method, selection from raw material, avoid in the leaching process dewatering and the ethanol use of other organic solvents outward, inventing a kind of is the novel technology for extracting that raw material extracts Radix Notoginseng total arasaponins with bright Radix Notoginseng, with the quality of raising Radix Notoginseng total arasaponins and the preparation method of extraction ratio.
A kind of method of extracting Radix Notoginseng total arasaponins from bright Radix Notoginseng is pulverized bright Radix Notoginseng, and soak with ethanol is extracted three times, and extracting solution reclaims ethanol, and concentrated solution is after treatment by macroporous adsorptive resins and alumina column, and eluent reclaims, concentrates, is drying to obtain.
Radix Notoginseng is the araliaceae ginseng plant.Put down in writing in Qing Dynasty's pharmacy works supplementary Amplifications of the Compendium of Materia Medica: " ginseng qi-tonifying the first, Radix Notoginseng enrich blood the first, the flavor with and merit also waits, so the title Panax pseudoginseng, be the most precious person in the Chinese medicine." Radix Notoginseng is described as " the refreshing medicine of hemostasis, the quality goods for the treatment of blood disorders " by ancient Chinese medicine doctor.Radix Notoginseng total arasaponins has obtained using widely with producing clinically, mainly is to use in the mode of oral medicine, therefore should avoid organic solvent residue as the Radix Notoginseng total arasaponins of raw material as far as possible.The extracting method of existing Radix Notoginseng total arasaponins has adopted organic solvent extractionprocesses such as acetone, n-butyl alcohol, makes that the Radix Notoginseng total arasaponins of extraction is residual an organic solvent that is difficult to eliminate.And existing extracting method all adopts dry Radix Notoginseng to extract as raw material, and according to result of study, the total saponin content of bright Radix Notoginseng is than the content high approximately 20% of dry Radix Notoginseng.This method adopts bright Radix Notoginseng to extract as raw material, has farthest guaranteed the complete of Radix Notoginseng total arasaponins.The comparison of the present invention and other Radix Notoginseng total arasaponins extraction process:
1, extract Radix Notoginseng total arasaponins through the present invention, can make more than the Radix Notoginseng total arasaponins extraction rate reached to 14%, other extracting method can only reach 10-12%.
2, the present invention adopts two kinds of adsorption columns to carry out adsorption treatment, effectively improves the purity of Radix Notoginseng total arasaponins, and three monomers of the present invention (R1+Rg1+Rb1) content can reach more than 70%, is higher than existing national standard (more than 60%).
3, the present invention is simple to operate, helps the quality control of product.
The specific embodiment:
A kind of method of from bright Radix Notoginseng, extracting Radix Notoginseng total arasaponins, concrete grammar is:
1. the bright Radix Notoginseng of raw material is cleaned, clean, surperficial moisture content is dried at shady and cool place, pulverizes, and is standby.
The bright Radix Notoginseng sample that 2. will make is put in the extraction pot, and 85% ethanol that adding 6-10 doubly measures carries out reflux, extract,, extracts 3 times, and 60 ℃ of temperature are filtered, and collect filtrate, and are standby.
3. merging filtrate concentrates, and vacuum decompression reclaims ethanol, and 60 ℃ of temperature get Radix Notoginseng extractum, and are standby.
4. the D101 macroporous adsorptive resins is passed through in Radix Notoginseng extractum suitable quantity of water dissolving back, resin demand is 1.3 times of raw material, uses earlier the distilled water eluting, and back 70% ethanol elution is collected ethanol elution, and is standby.
5. ethanol elution is reclaimed ethanol, be concentrated into driedly, by alumina column, use 70% ethanol elution behind the water dissolution, the collection effluent is concentrated into driedly, promptly gets Radix Notoginseng total arasaponins.
Claims (5)
1, a kind of method of from bright Radix Notoginseng, extracting Radix Notoginseng total arasaponins, it is characterized in that bright Radix Notoginseng is pulverized, soak with ethanol is extracted three times, and extracting solution reclaims ethanol, concentrated solution is after treatment by macroporous adsorptive resins and alumina column, and eluent reclaims, concentrates, is drying to obtain.
2, a kind of method of from bright Radix Notoginseng, extracting Radix Notoginseng total arasaponins according to claim 1, it is characterized in that alcohol extract concentration is 80% or 85% or 90% or 95%, D101 macroporous resin consumption is 1.1 or 1.2 or 1.3 or 1.4 times of raw material, and alumina column eluting concentration of alcohol is 60% or 70% or 80% or 90%.
3, a kind of method of extracting Radix Notoginseng total arasaponins from bright Radix Notoginseng according to claim 2 is characterized in that alcohol extract concentration is 85%, and D101 macroporous resin consumption is 1.3 times of raw material, and alumina column eluting concentration of alcohol is 70%.
4, a kind of method of extracting Radix Notoginseng total arasaponins from bright Radix Notoginseng according to claim 1 is characterized in that may further comprise the steps:
1. the bright Radix Notoginseng of raw material is cleaned, clean, surperficial moisture content is dried at shady and cool place, pulverizes, and is standby.
The bright Radix Notoginseng sample that 2. will make is put in the extraction pot, and the 80%-95% ethanol that adding 6-10 doubly measures carries out reflux, extract,, extracts 3 times, and 60 ℃ of temperature are filtered, and collect filtrate, and are standby.
3. merging filtrate concentrates, and vacuum decompression reclaims ethanol, and 60 ℃ of temperature get Radix Notoginseng extractum, and are standby.
4. the D101 macroporous adsorptive resins is passed through in Radix Notoginseng extractum suitable quantity of water dissolving back, resin demand is 1.1-1.4 a times of raw material, uses earlier the distilled water eluting, and back 70% ethanol elution is collected ethanol elution, and is standby.
5. ethanol elution is reclaimed ethanol, be concentrated into driedly, by alumina column, use the 60%-90% ethanol elution behind the water dissolution, the collection effluent is concentrated into driedly, promptly gets Radix Notoginseng total arasaponins.
5, a kind of method of extracting Radix Notoginseng total arasaponins from bright Radix Notoginseng according to claim 4 is characterized in that may further comprise the steps:
1. the bright Radix Notoginseng of raw material is cleaned, clean, surperficial moisture content is dried at shady and cool place, pulverizes, and is standby.
The bright Radix Notoginseng sample that 2. will make is put in the extraction pot, and 85% ethanol that adding 6-10 doubly measures carries out reflux, extract,, extracts 3 times, and 60 ℃ of temperature are filtered, and collect filtrate, and are standby.
3. merging filtrate concentrates, and vacuum decompression reclaims ethanol, and 60 ℃ of temperature get Radix Notoginseng extractum, and are standby.
4. the D101 macroporous adsorptive resins is passed through in Radix Notoginseng extractum suitable quantity of water dissolving back, resin demand is 1.3 times of raw material, uses earlier the distilled water eluting, and back 70% ethanol elution is collected ethanol elution, and is standby.
5. ethanol elution is reclaimed ethanol, be concentrated into driedly, by alumina column, use 70% ethanol elution behind the water dissolution, the collection effluent is concentrated into driedly, promptly gets Radix Notoginseng total arasaponins.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100581146A CN101513438A (en) | 2008-02-21 | 2008-02-21 | Method for extracting panax notoginseng saponins from fresh panax notoginsengs |
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| CNA2008100581146A CN101513438A (en) | 2008-02-21 | 2008-02-21 | Method for extracting panax notoginseng saponins from fresh panax notoginsengs |
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| CN101513438A true CN101513438A (en) | 2009-08-26 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104473765A (en) * | 2014-12-17 | 2015-04-01 | 文山学院文山三七研究院 | Forming process of pseudo-ginseng formula granules |
| CN104800258A (en) * | 2015-04-09 | 2015-07-29 | 广西大学 | Method for preparing panax notoginseng saponins |
| CN107595908A (en) * | 2017-09-26 | 2018-01-19 | 云南金七制药有限公司 | A kind of extracting method that notoginsenoside is extracted from fresh pseudo-ginseng |
| CN110663786A (en) * | 2019-11-20 | 2020-01-10 | 云南省农业科学院农产品加工研究所 | Processing method of pseudo-ginseng flower leaf tea beverage |
| CN110711210A (en) * | 2019-11-20 | 2020-01-21 | 云南省农业科学院农产品加工研究所 | Treatment method for improving saponin content of overground part of panax notoginseng |
| CN110720590A (en) * | 2019-11-26 | 2020-01-24 | 云南省农业科学院农产品加工研究所 | Preparation method of panax notoginseng aerial part nano functional rice |
-
2008
- 2008-02-21 CN CNA2008100581146A patent/CN101513438A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104473765A (en) * | 2014-12-17 | 2015-04-01 | 文山学院文山三七研究院 | Forming process of pseudo-ginseng formula granules |
| CN104473765B (en) * | 2014-12-17 | 2018-12-11 | 文山学院文山三七研究院 | A kind of moulding process of Radix Notoginseng granule |
| CN104800258A (en) * | 2015-04-09 | 2015-07-29 | 广西大学 | Method for preparing panax notoginseng saponins |
| CN107595908A (en) * | 2017-09-26 | 2018-01-19 | 云南金七制药有限公司 | A kind of extracting method that notoginsenoside is extracted from fresh pseudo-ginseng |
| CN110663786A (en) * | 2019-11-20 | 2020-01-10 | 云南省农业科学院农产品加工研究所 | Processing method of pseudo-ginseng flower leaf tea beverage |
| CN110711210A (en) * | 2019-11-20 | 2020-01-21 | 云南省农业科学院农产品加工研究所 | Treatment method for improving saponin content of overground part of panax notoginseng |
| CN110711210B (en) * | 2019-11-20 | 2022-01-07 | 云南省农业科学院农产品加工研究所 | Treatment method for improving saponin content of overground part of panax notoginseng |
| CN110720590A (en) * | 2019-11-26 | 2020-01-24 | 云南省农业科学院农产品加工研究所 | Preparation method of panax notoginseng aerial part nano functional rice |
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