CN101507659A - Preparation method of gastroesophageal and laryngotracheal regurgitation animal model - Google Patents
Preparation method of gastroesophageal and laryngotracheal regurgitation animal model Download PDFInfo
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- CN101507659A CN101507659A CNA2009100800523A CN200910080052A CN101507659A CN 101507659 A CN101507659 A CN 101507659A CN A2009100800523 A CNA2009100800523 A CN A2009100800523A CN 200910080052 A CN200910080052 A CN 200910080052A CN 101507659 A CN101507659 A CN 101507659A
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Abstract
The invention relates to a method for preparing a gastroesophago-laryngotracheal reflux animal model, and belongs to the field of medical biotechnology and experimental zoology. The method for preparing the gastroesophago-laryngotracheal reflux animal model comprises the following steps: fixing an experimental animal on an operating table, cutting the abdomen and the stomach wall of the experimental animal, inserting an esophageal dilator into an esophageal lumen from a stomach cut to cardiac ostium upward to the lower part of esophageal hiatus, and suturing and closing a continuous whole layer of the stomach cut with a seromuscular layer; and enclosing the periphery of an enteron by plastic sheets on duodenum position at the far end of pyloric ring, suturing and fixing the plastic sheets on mesentery, and suturing and dressing an abdomen cut to establish the gastroesophago-laryngotracheal reflux animal model. The method has the characteristics that the surgery has simple operation, the animal after the surgery survives for a long time, and a preparation principle is more close to clinical practice and supports functions of a reflux mechanism on GERD respiratory disorder.
Description
Technical field
The present invention relates to a kind of preparation method of gastroesophageal and laryngotracheal regurgitation animal model, belong to medical biotechnology field and experimental zoology field.
Background technology
(gastroesophageal reflux disease is a kind of functional gastrointestinal disorder of clinical common chronic, easy recurrence GERD) to gastroesophageal reflux disease, and its sickness rate is in rising trend in China.GERD is the digestive tract power obstacle disease that one kind of multiple factors cause, and its pathogenic factor and mechanism are not clear and definite fully as yet, therefore, for many years, people explore always set up a kind of objectively, the animal model of good reproducibility is studied the pathogeny of GERD as platform.Chen Ying, Wang Feng have summarized gastroesophageal reflux The Animal Model Study present situation, comprise the GERD animal model that destroys inferior esophageal sphincter, ligation pylorus or duodenal GERD animal model, the GERD animal model of esophagoduodenostomy and esophageal acid perfusion GERD animal model.(Chen Ying, Wang Feng, gastroesophageal reflux The Animal Model Study present situation, Chin JGastroenterol, 2006,11 (9): 568-570.) this summary described in the molding mode various, but the standard of animal model success is confined to the pathological change of reflux esophagitis more, and in recent years, gastroesophageal reflux source property respiratory disorder, the particularly pathogeny of " asthma " are subjected to the concern and the research of each side.
China Wang Zhong Ho academician in 2007 in conjunction with total institute of the Second Artillery Force of PLA gastroesophageal reflux center accept for medical treatment 200 surplus routine GERD patient's clinical characters, the notion of initiative proposition stomach esophagus larynx trachea syndrome.(Wang Zhonggao, Liu Jianjun, Chen Xiu etc., the discovery and the name of stomach esophagus larynx trachea syndrome (GELTS), clinical misdiagnosis wrong treatment, 2007,20 (5): 5-9.) he describes this notion in the text like this: " and by GERD cause with the bottleneck throat be core, often with respiratory tract show especially asthma, larynx tracheospasm be projecting point, relate to and breathe and digest a series of corresponding clinical syndrome (but wilfully can not having heartburn and acid regurgitation) in two big systems and ear muzzle chamber; tentatively be called stomach esophagus larynx trachea syndrome (gastroesophagolarygotracheal syndrome; GELTS syndrome) or two pipe one chamber syndromes (Two track-one cavity syndrome), seemingly be not." he thinks: the clinical manifestation of GERD may be summarized to be three classes: esophagus, respiratory and oral cavity property.And point out that cavum laryngis is the hinge of contact digestion respiratory system from dissecting not only, core organ for the GELTS syndrome, gastric content sprays through this and divides to oropharynx and air flue, and the dysfunction of cavum laryngis is the basis and the prerequisite of the outer pathological changes of other esophaguses, the outer numerous and jumbled situation of symptom of esophagus has been corrected in the proposition of this notion undoubtedly, makes this disease that a direct clear and definite notion be arranged.Simultaneously, he is according to the experience to observation and self ill diagnosis and treatment of clinical patients, point out that further gastroesophageal reflux source property " asthma " is not real asthma, it is real to be respiratory distress (the Zhonggaowang.A belated revelation:from gastroesophageal reflux derivedasthma to laryngotracheal irritation even spasm.Frontiers of medicine inchina.2008 (2): 127-129.) that the directly anti-stream excitation of acidic contents larynx air flue causes.(Wang Zhonggao.The announcement that comes late: from gastroesophageal reflux source property asthma to larynx air flue excitation even spasm.The Chinese medicine forward position.2008(2)：127-129)。Therefore, anti-stream mechanism gets further research to the influence value of GERD source property breathing illness, and for a long time, the critical role of reflex mechanism in the respiratory tract disease that gastroesophageal reflux disease causes by the vagus nerve mediation established, people ignore the effect of anti-stream mechanism in the property respiratory disorder of GERD source, in recent years, pepsic discovery is considered to prove the strong evidence of anti-stream mechanism in the GERD patient airway and in the lung-douching fluid, people begin to pay attention to influence and the inherent mechanism thereof of direct anti-stream mechanism to respiratory tract, and it is a kind of suitable to be necessary to set up, the anti-flow model of stomach esophagus larynx air flue that meets pathogenesis is to provide research platform.
Inspire in above-mentioned background, need set up a kind of objective, generally acknowledge and the animal model of good reproducibility, be the cause of disease and the pathogenesis of further clear and definite GERD source property respiratory distress, in the hope of obtaining better clinical treatment method.Inventor early stage this has been carried out exploring (. transport steel, Wang Zhonggao, Wu Jimin etc.The anti-stream of stomach esophagus larynx trachea inquired in zoopery.China's experimental surgery magazine.2008；25(10)：1352-1353)。Destroy the inferior esophageal sphincter of rat, ligation blocking-up pyloric ring, gastric injects methylene blue solution, and we find that appearance indigo plant is in various degree dyed in the experimental group larynx air flue postoperative, in conjunction with clinical observation, we think that the anti-stream mechanism through dissecting approach is one of important cause of disease of GERD source property breathing illness.But too short with this rat postoperative life span for preparing the model preparation, 10 groups of average postoperative life spans of rat are 22 hours, and wherein one only is 2 hours.And ligation blocking-up pyloric ring, the outlet of complete closed stomach, though can cause gastric content acutely instead to flow rapidly, preparation method and clinical paathogenic factor are not inconsistent, and lack persuasion.
At present the gastroesophageal reflux animal model is paid attention to the research of reflux esophagitis more, property breathing illness in anti-stream source is lacked inquire into, and also comparatively is fit to the animal model that the anti-stream mechanism of research acts on and is in the news in the property respiratory distress of GERD source.
Technology contents
The technical problem to be solved in the present invention is: the preparation method that a kind of gastroesophageal and laryngotracheal regurgitation animal model is provided.
For achieving the above object, the present invention is by the following technical solutions:
A kind of preparation method of gastroesophageal and laryngotracheal regurgitation animal model, it is characterized in that: laboratory animal is fixed on the operating-table, hara kiri also cuts the coat of the stomach of laboratory animal, esophageal dilator is inserted esophageal lumen through the stomach otch to cardia, upwards to the esophageal hiatus below, the continuous holostrome of stomach otch adds seromuscular layer suture and closes; The place holds the intestinal tube periphery with sheet in pyloric ring far-end duodenum, and seam pricks and to be fixed in mesentery, sews up abdominal incision and wrapping, sets up gastroesophageal and laryngotracheal regurgitation animal model.
Described laboratory animal is a rat.
Described hara kiri and the method for cutting the laboratory animal coat of the stomach are: get median abdominal incision, arise from the long 5cm of xiphoid-process downwards, advance abdomen and appear paries anterior gastricus, 1cm avascular area longitudinal incision coat of the stomach under the stomach esophagogastric junction, long incision 5mm.
The described plastic cement length of a film that holds intestinal tube is 14～15mm, and wide is 6～8mm.
The pyloric ring far-end duodenum place that is set forth in the method that sheet holds the intestinal tube periphery is: be close to intestinal tube annular to duodenum place between the pyloric ring far-end 1.5cm with sheet in pyloric ring and hold the intestinal tube periphery, form circulus, this place's intestinal tube diameter is 4～5mm before holding, and the diameter of this circulus is consistent with the diameter that holds preceding this place's intestinal tube after holding.
Described esophageal dilator is tubaeform.This esophageal dilator back cut diameter is 3mm, and lower port diameter is 5mm, and pipe range is 25mm.
Described laboratory animal can also be rabbit, dog, sheep or pig.The diameter of esophageal dilator and length, the concrete big I of sheet is given and change according to the practical situation of different animals, and also can utilize sheet that corresponding animal intestinal is carried out multistage to hold, cause intestinal dyskinesis in various degree.
The preparation method of gastroesophageal and laryngotracheal regurgitation animal model of the present invention can come the simulation experiment animal to carry out model construction and correlational study with synthetical animal pattern.
Characteristics of the present invention are:
(1) the inferior esophageal sphincter functional impairment is the pathogenic anatomic basis of gastroesophageal reflux, and the esophagus power abnormality also is important paathogenic factor simultaneously.GERD patient with respiratory symptom is carried out the esophagus dynamic test, the row interior PH dynamic monitoring of esophagus in 24 hours simultaneously, find all to have the esophagus power abnormality, it is all closely related that acid exposes total time, clinostatism time, clinostatism esophageal acid checkout time in particularly invalid esophagus power and the esophagus, thinks that the esophagus power abnormality plays an important role in the pathogenesis with the GERD patient of respiratory symptom.
In the animal model preparation method of the present invention, esophageal dilator places in the esophageal lumen through the stomach otch, and length until the esophageal hiatus below, is crossed over distal esophagus by stomach esophagogastric junction place fully, offset the function of inferior esophageal sphincter, cause the inferior esophageal sphincter afunction.The tube wall of esophagus hypomere own is close to the dilator periphery simultaneously, the passive tractive of muscle fiber extends, this esophagus power function is impaired, dilator is implanted the esophagus section and is in invalid esophagus dynamic regime, and distal esophagus is the vagus nerve comparatively intensive zone that distributes, and reflex mechanism is many brings into play effect by gastric content to this regional vagus nerve excitation.Esophageal dilator maskable stomach acidity content in this model is evaded the effect of reflex mechanism to respiratory tract fully to the vagal stimulation of distal esophagus portion, only with regard to direct anti-stream mechanism observational study is carried out in the effect of respiratory tract.We also can remove the shielding of dilator to distal esophagus in various degree by changing the length that reduces dilator simultaneously, observe reflection and the anti-stream mechanism dual function to respiratory system simultaneously.
(2) along with to the deepening continuously of GERD research, it should be noted that many GERD patients exist simultaneously big belly, early full, belch, feel sick, gastric motility disorder symptom such as vomiting.Prompting GERD patient may exist the stomach motor function impaired, and gastrointestinal dysfunction is one of important paathogenic factor of gastroesophageal reflux.Studies show that there is delayed gastric emptying in about 33% GERD patient.Radioisotope scanning finds that part GERD patient exists gastric liquid, solid emptying to postpone.And after using dynamics-promoting medicine treatment or anti-reflux operative treatment, remission, it is normal that gastric emptying recovers.To patient GERD carry out before the meal, stomach electricity of body surface figure (EGG) monitoring after the meal, find that GERD patient exists before the meal, gastric electrical activity is unusual after the meal, illustrates that delayed gastric emptying is relevant with gastroesophageal reflux.Among some patient who accepts for medical treatment, the patient causes the gastroduodenal dyskinesis because of mesentery perstriction duodenum, and anti-stream of gastric content caused the class symptoms of asthma, and after operation releasing intestinal blocked, the patient respiratory symptom was obviously improved.
In the animal model preparation method of the present invention, in pyloric ring far-end duodenum place with the sheet parcel intestinal tube periphery of going in ring, it is narrow not cause parcel section enteric cavity, but during the laboratory animal feed, can suppress local intestinal peristalsis promoting, make the gastrointestinal emptying dysfunction, show and dissect when laboratory animal is dead: parcel section intestinal tube is unobstructed, and its nearly section intestinal tube and flatulence include food entrapment, the preparation method that the animal model distal gut is described can cause the gastrointestinal emptying obstacle, thereby promotes the anti-stream of gastric content.And previously in the animal model preparation method of reflux esophagitis, adopt with surgical operation causes stomach pylorus, duodenum fully or partial obstruction more, thereby constitutes the key factor that promotes the anti-stream of gastroduodenal content.And this model adopts sheet to wrap up local enteric cavity; the mechanicalness that does not cause local intestinal is narrow; the protection enteric cavity is unobstructed naturally, but animal when feed, parcel intestinal tube part wriggling function is restricted; local food moves in intestinal and slowly is obstructed; directly cause the emptying obstacle, thereby constitute the anti-stream factor of gastric content, and can be by the width of regulating sheet or the parcel that adopts the multistage intestinal; thereby change the length of the intestinal tube that is wrapped, and then change the degree of intestinal emptying obstacle.Simultaneously, our clinical observation thinks that GERD patient's gastrointestinal dysfunction is the important paathogenic factor of gastroesophageal reflux, and this and this Preparation of model principle match.
In addition, the sheet of intestinal tube parcel in the animal model of the present invention can change stomach esophagus emptying obstacle degree according to the packages length or the row multistage intestinal tube parcel of experiment situation change sheet.
(3) with the anti-fluid preparation method of setting up early stage of stomach esophagus larynx trachea, promptly destroy the inferior esophageal sphincter of rat, ligation blocking-up pyloric ring, gastric injects the animal model of methylene blue solution and compares: use this animal model successfully to prepare 10 rats, behind rats death, all can observe the existence that gastric content is arranged in its larynx air flue, and there is airway secretions to occur, illustrate that this kind preparation method is the anti-preparation method that flows of a kind of successful stomach esophagus larynx air flue, for the GERD source property airway inflammation due to the anti-stream mechanism of further research provides the laboratory animal platform.The animal preparation method of setting up early stage, the animal system type success back existence time limit obviously prolongs, and long time limit can reach 6 days during the feed food, if adopt the liquid nutritional feeding, has and continues possibility of feeding, and is convenient to observe the course of disease and makes progress.In this animal model, by sphincter function under the broken annular eclipse pipe, the forfeiture of esophagus wriggling function, the gastrointestinal emptying obstacle meets clinical practice more as the preparation principle basis.
The invention will be further described below in conjunction with the specific embodiment; be not the qualification to invention, according to prior art well known in the art, embodiments of the present invention are not limited to this; therefore all this areas of having done according to present disclosure be equal to replacement, all belong to protection scope of the present invention.
Description of drawings
Fig. 1: the anti-stream of larynx air flue of rats in test groups, arrow indication are that the anti-larynx air flue that flow to of gastric serge blue causes that local indigo plant is dyed and the figure of secretions formation;
Fig. 2: the anti-stream of larynx air flue of rats in test groups, arrow indication are that the anti-larynx air flue that flow to of gastric serge blue causes that local indigo plant is dyed and the figure of secretions formation.
The specific embodiment
Embodiment 1: the modeling experiment
One. laboratory animal and grouping:
Rat (Sprague-Dawley, SD), male and female half and half, body weight be 250～300 the gram, provide by Capital University of Medical Sciences's Experimental Animal Center.Totally 20.The raising condition: 23～25 ℃ of room temperatures, to put in the rat Rotating Stainless Steel Cage tool and raise, 5 in every cage is freely taken the photograph water and is ingested.
2.20 only laboratory animal adopts the method for random packet to be divided into two groups, experimental group and matched group, 10 every group.
Two. modelling:
Experimental group: fasting 24h before the art, can't help water.With the administration of anaesthetic of 10% chloral hydrate (2.5-3ml/kg) intraperitoneal injection.Animal is got dorsal position, and extremity and head are fixed in operating-table.Art district preserved skin drape such as routine.Get median abdominal incision, arise from the long 5cm of xiphoid-process downwards, advance abdomen and appear paries anterior gastricus, the long 5mm of 1cm avascular area longitudinal incision coat of the stomach under the stomach esophagogastric junction, with back cut diameter 3mm, lower port diameter 5mm, the tubaeform esophagectasis pipe of pipe range 25mm slowly inserts esophageal lumen (distal esophagus external diameter 2-3mm) through the stomach otch to the duty doorway, upwards to the esophageal hiatus below, the continuous holostrome of otch adds seromuscular layer suture and closes.(desire parcel place intestinal tube diameter is 4～5mm) at the duodenum place between 1.5 centimetres in pyloric ring to the pyloric ring far-end, be close to the long 14mm of intestinal tube, the sheet annular of wide 7mm holds, form circulus, the diameter of this circulus is consistent with the diameter that holds preceding this place's intestinal tube, and the seam bundle is fixed in mesentery.Sew up abdominal incision and wrapping.
Matched group: after advancing abdomen, simply stir intestinal tube, sew up abdominal incision and wrapping.
Embodiment 2: the modeling experiment
One. laboratory animal and grouping:
Rat (Sprague-Dawley, SD), male and female half and half, body weight be 250～300 the gram, provide by Capital University of Medical Sciences's Experimental Animal Center.Totally 20.The raising condition: 23～25 ℃ of room temperatures, to put in the rat Rotating Stainless Steel Cage tool and raise, 5 in every cage is freely taken the photograph water and is ingested.
2.20 only laboratory animal adopts the method for random packet to be divided into two groups, experimental group and matched group, 10 every group.
Two. modelling:
Experimental group: fasting 24h before the art, can't help water.With the administration of anaesthetic of 10% chloral hydrate (2.5-3ml/kg) intraperitoneal injection.Animal is got dorsal position, and extremity and head are fixed in operating-table.Art district preserved skin drape such as routine.Get median abdominal incision, arise from the long 5cm of xiphoid-process downwards, advance abdomen and appear paries anterior gastricus, the long 5mm of 1cm avascular area longitudinal incision coat of the stomach under the stomach esophagogastric junction, with back cut diameter 3mm, lower port diameter 5mm, the tubaeform esophagectasis pipe of pipe range 25mm slowly inserts esophageal lumen (distal esophagus external diameter 2-3mm) through the stomach otch to cardia, upwards to the esophageal hiatus below, the continuous holostrome of otch adds seromuscular layer suture and closes.(desire parcel place intestinal tube diameter is 4～5mm) at the duodenum place between 1.5 centimetres in pyloric ring to the pyloric ring far-end, be close to the long 15mm of intestinal tube, the sheet annular of wide 8mm holds, form circulus, the diameter of this circulus is consistent with the diameter that holds preceding this place's intestinal tube, and the seam bundle is fixed in mesentery.Sew up abdominal incision and wrapping.
Matched group: after advancing abdomen, simply stir intestinal tube, sew up abdominal incision and wrapping.
Embodiment 3: stomach esophagus larynx trachea is counter to flow research
1. method:
After making up model as method as described in the embodiment 1, every all single cage of rat is placed also and is observed, and water is prohibited in postoperative fasting in first day, and second day after operation is given and methylene blue solution (Jiangsu JumpCan Medicines Co., Ltd.), and postoperative began to expecting with the small quantities of particles Mus on the 3rd day.
2. result:
Experimental group: the postoperative life span is 3-6 days, and dead the dissection shown: 10 slight in various degree light dying of rat larynx air flue, visible foam sample secretions in the air flue wherein has small amounts of food residual in 2 rat larynx air flues.Two lungs there is no unusually.Esophageal dilator and sheet all do not come off, and stomach and esophagus indigo plant in various degree dye, and stomach is expanded in various degree, in have food residual, the nearly section of sheet parcel dilatation of intestine, but parcel section enteric cavity is unobstructed, the all visible indigo plant of Mus body is dyed vestige, and visible indigo plant is dyed vestige and feces retains at the bottom of the mouse cage.See Table 1
Matched group: postoperative survival is good, puts to death in postoperative 6 days, and dissection is shown: the larynx air flue there is no that indigo plant is dyed and airway secretions, two lung no abnormality seens, gastroduodenal no abnormality seen.See Table 2.
Table 1: the anti-result that flows of experimental group
| Mus number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Stomach maximum transverse diameter (during art) cm | 2.3 | 2.2 | 2.2 | 2.2 | 2.1 | 2.3 | 2.2 | 2.3 | 2.0 | 2.3 |
| The Weishang is footpath (during art) cm down | 2.2 | 1.9 | 1.8 | 1.6 | 2.0 | 1.9 | 1.8 | 1.9 | 1.9 | 1.9 |
| Stomach maximum transverse diameter (during death) cm | 3.4 | 2.7 | 3.5 | 3.5 | 2.6 | 2.8 | 2.3 | 3.0 | 2.2 | 3.5 |
| The Weishang is footpath (during death) cm down | 2.0 | 2.0 | 2.2 | 1.7 | 2.1 | 2.1 | 2.0 | 2.5 | 1.0 | 2.5 |
| Duodenum diameter (during art) cm | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.4 | 0.4 | 0.5 | 0.5 | 0.5 |
| Duodenum diameter (during death) cm | 0.8 | 0.7 | 0.7 | 0.7 | 0.7 | 0.6 | 0.5 | 0.7 | 0.7 | 0.8 |
| Indigo plant is dyed in the larynx air flue | Have | Have | Have | Have | Have | Have | Have | Have | Have | Have |
| Pulmonary's indigo plant is dyed | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have |
| Life span (my god) | 3 | 4 | 4 | 5 | 5 | 6 | 2 | 3 | 3 | 5 |
Annotate: the stomach maximum transverse diameter is as the criterion with stomach horizontal line longest distance, and the footpath is as the criterion with the longest distance of gastroesophageal junction on vertical line up and down.Duodenum diameter: sheet parcel near-end intestinal tube external diameter.1st, it is residual to can be observed small amounts of food in 8 larynx air flues.
Table 2: the anti-result that flows of matched group
| Mus number | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Indigo plant is dyed in the larynx air flue | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have |
| Pulmonary's indigo plant is dyed | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have | Do not have |
| The execution time (my god) | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
Compare with matched group, all visible indigo plant is dyed in the experimental group rat larynx air flue, and visible foam shape secretions even gastric food are seen accompanying drawing 1,2 in the part rat airway.Illustrative experiment group distal esophagus is implanted dilator, cause inferior esophageal sphincter afunction and esophagus power abnormality, more than the lasting existence and the effect of two kinds of paathogenic factors add the gastrointestinal emptying obstacle, impel in the experimental group the outer direct anti-stream of esophagus to reach at utmost, gastric content can instead flow to the larynx air flue, and air flue is subjected to instead flowing the invasion and attack excitation of thing and merocrine secretion's thing being increased.Simultaneously also further specifying direct anti-stream mechanism is one of important mechanisms of GERD source property breathing illness.
Claims (8)
1. the preparation method of a gastroesophageal and laryngotracheal regurgitation animal model, it is characterized in that: laboratory animal is fixed on the operating-table, hara kiri also cuts the coat of the stomach of laboratory animal, esophageal dilator is inserted esophageal lumen through the stomach otch to cardia, upwards to the esophageal hiatus below, the continuous holostrome of stomach otch adds seromuscular layer suture and closes; The place holds the intestinal tube periphery with sheet in pyloric ring far-end duodenum, and seam pricks and to be fixed in mesentery, sews up abdominal incision and wrapping, sets up gastroesophageal and laryngotracheal regurgitation animal model.
2. the preparation method of a kind of gastroesophageal and laryngotracheal regurgitation animal model according to claim 1, it is characterized in that: described laboratory animal is a rat.
3. the preparation method of a kind of gastroesophageal and laryngotracheal regurgitation animal model according to claim 1 and 2, it is characterized in that: described hara kiri and the method for cutting the laboratory animal coat of the stomach are: get median abdominal incision, arise from the long 5cm of xiphoid-process downwards, advance abdomen and appear paries anterior gastricus, 1cm avascular area longitudinal incision coat of the stomach under the stomach esophagogastric junction, long incision 5mm.
4. the preparation method of a kind of gastroesophageal and laryngotracheal regurgitation animal model according to claim 1 and 2, it is characterized in that: the described plastic cement length of a film that holds intestinal tube is 14～15mm, wide is 6～8mm.
5. the preparation method of a kind of gastroesophageal and laryngotracheal regurgitation animal model according to claim 1 and 2, it is characterized in that: the pyloric ring far-end duodenum place that is set forth in the method that sheet holds the intestinal tube periphery is: be close to intestinal tube annular to duodenum place between the pyloric ring far-end 1.5cm with sheet in pyloric ring and hold the intestinal tube periphery, form circulus, this place's intestinal tube diameter is 4～5mm before holding, and the diameter of this circulus is consistent with the diameter that holds preceding this place's intestinal tube after holding.
6. the preparation method of a kind of gastroesophageal and laryngotracheal regurgitation animal model according to claim 1 and 2, it is characterized in that: described esophageal dilator is tubaeform.
7. the preparation method of a kind of gastroesophageal and laryngotracheal regurgitation animal model according to claim 6, it is characterized in that: described tubaeform esophageal dilator back cut diameter is 3mm, and lower port diameter is 5mm, and pipe range is 25mm.
8. the preparation method of a kind of gastroesophageal and laryngotracheal regurgitation animal model according to claim 1, it is characterized in that: described laboratory animal can also be rabbit, dog, sheep or pig.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110612940A (en) * | 2019-09-24 | 2019-12-27 | 延安大学 | Modeling method of bile reflux gastric injury animal model |
| CN112164270A (en) * | 2020-10-22 | 2021-01-01 | 北京博医时代教育科技有限公司 | Training model for abdominal peritoneal hernia repair operation and manufacturing method and storage method thereof |
-
2009
- 2009-03-18 CN CNA2009100800523A patent/CN101507659A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110612940A (en) * | 2019-09-24 | 2019-12-27 | 延安大学 | Modeling method of bile reflux gastric injury animal model |
| CN112164270A (en) * | 2020-10-22 | 2021-01-01 | 北京博医时代教育科技有限公司 | Training model for abdominal peritoneal hernia repair operation and manufacturing method and storage method thereof |
| CN112164270B (en) * | 2020-10-22 | 2022-04-15 | 北京博医时代教育科技有限公司 | Training model for abdominal peritoneal hernia repair operation and manufacturing method and storage method thereof |
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