CN101502546A - Ginseng (root) oligosacchride preparation and novel use thereof - Google Patents
Ginseng (root) oligosacchride preparation and novel use thereof Download PDFInfo
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- CN101502546A CN101502546A CNA2009100665625A CN200910066562A CN101502546A CN 101502546 A CN101502546 A CN 101502546A CN A2009100665625 A CNA2009100665625 A CN A2009100665625A CN 200910066562 A CN200910066562 A CN 200910066562A CN 101502546 A CN101502546 A CN 101502546A
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Abstract
The invention relates to ginseng mixed oligosaccharides which are extracted and separated from ginseng root by a macroporous resin column, a hollow fiber column for ultrafiltration, an activated carbon column for adsorption and purification and other technologies, and the ginseng mixed oligosaccharides is proved to have new purposes of anti-senile dementia, analgesic and anti-gastric ulcer.
Description
Technical field
The present invention relates to the ginseng oligosaccharide that obtains by extraction separation in the Radix Ginseng (root), i.e. the Radix Ginseng mixing oligosaccharide formed by 2-15 monosaccharide of the degree of polymerization, as anti-senile dementia medicine, analgesic, the application of gastric ulcer resistance medicine aspect
Background technology:
Radix Ginseng is the dry root of Araliaceae Radix Ginseng (Panax ginsengC.A.Meyer).Modern certainly from Gu as rare medicinal herbs, because its unique medical care effect, be subjected to people's attention deeply always, in order more effectively to develop this valuable ingredient of Chinese medicine, for many years, both at home and abroad multidisciplinary multi-field in to the Radix Ginseng chemical constituent and active aspect carried out number of research projects, therefrom isolated main component has saponins, polysaccharide, polypeptide class, fatty acid, amino acids, volatilization wet goods, and it is the most extensive that ginsenoside wherein and ginseng polysaccharide's constituents are studied aspect biological activity.For a long time, because people know little to the function of oligosaccharide, so very limited to aspect researchs such as the separation of Radix Ginseng (root) oligosaccharide and biological activitys.
In recent years, very active to the research of functional oligosaccharide both at home and abroad, its major physiological function embodiment is 1) promote bacillus bifidus propagation, suppress the growth and breeding of enteric pathogenic bacteria; 2) effect of poisonous tunning of minimizing and noxious bacteria enzyme; 3) prevention, treatment constipation, diarrhoea; 4) remove harmful substance, the liver protecting function; 5) reduce serum cholesterol and blood pressure; 6) raise immunity has anti-cancer and kill cancer action; 7) anti-aging effects; 8) promote bacillus bifidus to generate nutrient substance such as vitamin; 9) prevention and treatment epileptics; 10) has the function of water soluble dietary fiber; 11) promote Ca
2+Absorption; 12) suppressing cancer takes place; 13) treatment of nervous system disease.As seen, the oligosaccharide product all shows the wide development prospect in fields such as food, health care, medicine, chemical industry, and this compounds is that developing new drug and health product have been widened the space.
Summary of the invention
The invention provides a kind of from Radix Ginseng (root) preparation technology of extraction separation Radix Ginseng mixing oligosaccharide, and the product of this explained hereafter is as anti-senile dementia medicine, analgesic, gastric ulcer resistance medicine.
To achieve these goals, the invention provides following technical scheme:
1. preparation Radix Ginseng mixture oligosaccharide is pulverized Radix Ginseng (root), adds the water that 10-15 doubly measures, and decocts 3 hours, decocts repeatedly 2 times, and filtrate merges, and is concentrated into 1 gram medical material/milliliter, crosses macroporous resin D
101Being washed to elution volume is 2 times of preceding amounts of upper prop, water elution liquid is by hollow fiber column (molecular cut off 3000) ultrafiltration, the outer liquid of ultrafiltration concentrates, concentrated solution (1 gram medical material/milliliter) is gone up activated-charcoal column through the alcohol grading eluting, collect 30% ethanol elution part, concentrate, vacuum drying gets Radix Ginseng mixing oligosaccharide.
Radix Ginseng mixture oligosaccharide structure be verified as the structure of determining each monomer oligosaccharide in the Radix Ginseng mixture oligosaccharide, we utilize spectroscopic data to verify that the product of above-mentioned explained hereafter is Radix Ginseng mixing oligosaccharide, are made up of 2-7 sugar, analysis result is as follows:
1. physicochemical property result
The mensuration of total sugar content adopts the phenolsulfuric acid method, and glucose is product in contrast, the production standard curve, and the result shows that the total sugar content in the Radix Ginseng mixing oligosaccharide is 78%.
Between the acid sugar Determination on content adopts-and the xenol method, galacturonic acid is product in contrast, the production standard curve, the result shows that the acid sugar content in the Radix Ginseng mixing oligosaccharide is 12.9%
The mensuration of protein content adopts the Lowry method, and bovine serum albumin is product in contrast, the production standard curve, and the result shows that the protein content in the Radix Ginseng mixing oligosaccharide is 6.8%.
2.. molecular weight distribution adopts HPLC, the differential detector, 0Hpak SB-802.5HQ gel column, the Dextru of different molecular weight is product in contrast, the production standard curve, the result shows that Radix Ginseng mixing oligosaccharide molecular amount mainly is distributed between the 3000-300.
3.. form glycan analysis and adopt the sugar alcohol acetylation method that Radix Ginseng mixing oligosaccharide is prepared into derivant; with the derivant of each monosaccharide product in contrast; GC result shows that Radix Ginseng mixing oligosaccharide is made up of rhamnose, arabinose, xylose, galactose, glucose, glucuronic acid, galacturonic acid.
4.. the Hakomori method is adopted in the interpretation of result that methylates, and GC-MS result shows, the glycosidic bond connected mode of Radix Ginseng mixing oligosaccharide, mainly with 1-4,1-6 is connected to main chain.
5. .ESI-MS carries out mass spectral analysis to ginseng oligosaccharide, is made up of 2-7 monosaccharide by the Radix Ginseng mixing oligosaccharide that fragment peak can be inferred.Be subjected to the restriction of condition, the oligosaccharide that molecular weight is bigger still can not be used this method.
Claim
1. the product of 2. pairs of these explained hereafter of preparation technology of extraction separation Radix Ginseng mixing oligosaccharide from Radix Ginseng (root) is all given protection as the product of 4. pairs of these explained hereafter of analgesic as medicament for anti-gastric ulcer as the product of 3. pairs of these explained hereafter of anti senile dementia drug.
Embodiment
1. Radix Ginseng mixing oligosaccharide obtains by following two kinds of extracting method:
Method 1 is got Radix Ginseng 5Kg, is ground into coarse powder, adds the water of 10 times of amounts, decocts 3 hours, and 2 times repeatedly, filtrate is concentrated into 5000 milliliters, crosses macroporous resin D
101Post, water elution to 10000 milliliter, water elution liquid ultrafiltration (hollow fiber column, molecular cut off 3000), ultrafiltrate concentrates, and drying promptly gets Radix Ginseng mixture oligosaccharide 75g.
Method 2 is got Radix Ginseng 5Kg, is ground into coarse powder, adds the water of 15 times of amounts, decocts 3 hours, and 2 times repeatedly, filtrate is concentrated into 5000 milliliters, crosses macroporous resin D
101Post, water elution to 10000 milliliter, water elution liquid is concentrated into 1000 milliliters, adds ethanol to determining alcohol 60-80%, and alcoholic solution reclaims ethanol, concentrates, and drying promptly gets Radix Ginseng mixture oligosaccharide 67g.
2. the activity test of Radix Ginseng mixing oligosaccharide and result
(1) Radix Ginseng mixing oligosaccharide defying age test
Influenced the scopolamine induced mice is amnemonic by reagent
Grouping and administration
Normal control group water 20ml/kg
Model group water 20ml/kg
The positive drug group is than La Xitan 700mg/kg
Radix Ginseng mixing oligosaccharide 400mg/kg
Get 40 of Kunming mouses, be divided into 4 groups at random, be respectively the blank group, model group, positive drug is than La Xitan and administration group, except that normal control group and model group administration and isopyknic water, each organizes mice every day, and fixedly the period gastric infusion is once, continuous 6d, each group of 3d is normally carried out 4 of water maze and is resembled the training of line orientation navigation, 4d, 5d before the administration, 30min tests after the 6d administration, remove normal control group intraperitoneal injection of saline 10ml/kg, all the other respectively organize all lumbar injection scopolamine 2mg/kg before training of mice, and the observed and recorded mice was found the used time of platform (escape latency) in 120 seconds, totally four resemble line, average.7d carries out space exploration test, removes platform, makes mice seek platform in the memory under no platform situation, and the 120s that swims writes down the cross-platform number of times of mice.In whole test process, the peripheral pool object of reference comprises the invariant position of experimenter own, and the result is as follows:
Be subjected to reagent to the amnemonic result of scopolamine induced mice (x ± s, n=10)
| Group | 4d after the administration (escape latency) (second) | After the administration the 5th (escape latency) (second) | 6d after the administration (escape latency) (second) | The number of times of spanning platform |
| The normal control group | 70.7688± 10.10787 | 74.3383± 11.11422 | 77.4289± 8.11770 | 2.0500± 0.22298 |
| Model group | 95.9607± 8.15643 | 96.6482± 10.0276 | 102.37± 5.16331 | 1.8750± 0.35038 |
| The positive drug group | 86.3474± 9.71912 | 92.8394± 7.58107 | 87.2650± 6.77475 | 2.2750± 0.64706 |
| The administration group | 76.6669± 8.67879 | 90.0024± 9.05353 | 76.7582± 9.44077 | 1.9250± 0.42500 |
The result shows, compares with model group, and positive drug group effect is remarkable, and administration group and model group comparison are obviously shortened in the 4th day escape latency of administration, and the spanning platform number of times increases, and the 5th, 6 day more steady.p<0.01。
(2) Radix Ginseng mixing oligosaccharide analgesic test
The receptor medicine is to the influence of mice acetic acid twisting
Grouping and administration
Model group 20ml/kg (solvent)
Radix Ginseng mixing oligosaccharide 400mg/kg
Get 20 of Kunming mouses, be divided into 2 groups at random, be respectively model group, administration group, each organize mice every day fixedly the period gastric infusion once, model group is given and isopyknic solvent, continuously 7d.After the last administration 1 as a child, the acetic acid of every mouse peritoneal injection 0.6% is observed and is turned round the body number of times in the mice 20 minutes, and the result is as follows:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |
| Turn round the body number of times in 20 minutes for one group | 23 | 8 | 18 | 7 | 12 | 7 | 8 | 16 | * | 14 |
| Turn round the body number of times in 20 minutes for two groups | 4 | 8 | 4 | 13 | 2 | 10 | 6 | 7 | 18 | 3 |
The mean value standard error: one group is 12.556 ± 1.886; Two group 7.500 ± 1.579.The result shows that the number of times of turning round body in the administration group mice 20min obviously is less than model group, p＜0.05.
(3) Radix Ginseng mixture oligosaccharide antiulcer test
Influence to stress in rats type ulcer
With the rat random packet, irritating first after stomach (po) administration is fasting, and be administered once next day again, totally 2 times.1h after the last administration, with rat upside down, extremity are tied on the yoke.Behind the 16h, put to death animal, cut open the belly, the ligation pylorus takes out full stomach, injects the 10ml normal saline, the ligation cardia.Then full stomach is soaked in 1% formalin fixing.Cut off stomach along lesser gastric curvature, lie on the glass plate.Press literature method evaluation ulcer grade.Statistics shows that Radix Ginseng mixture oligosaccharide all has obvious inhibitory action to stress in rats type ulcer, and the result is as follows.
Radix Ginseng mixing oligosaccharide is to the effect of stress in rats type ulcer
Claims (3)
1. Radix Ginseng (Panax ginsengC.A.Meyer) (root) is pulverized, added the decocting that 10-15 doubly measures and boil 2 times, decocted 3 hours at every turn, filter, filtrate merges, and is concentrated into 1 gram medical material/milliliter, D
101Macroporous resin, 2 times of amounts of water eluting column volume, water elution liquid is by hollow fiber column (molecular cut off 3000) ultrafiltration, ultrafiltrate concentrates, concentrated solution (1 gram medical material/milliliter) is gone up activated-charcoal column through the alcohol grading eluting, collects 30% ethanol elution part, concentrates, vacuum drying gets Radix Ginseng mixing oligosaccharide.
2. the spy's card according to the described Radix Ginseng mixing of claim 1 oligosaccharide is: (a) total sugar content in the Radix Ginseng mixing oligosaccharide is 78%, and acid sugar content is 12.9%, protein 6.8%; (b) Radix Ginseng mixing oligosaccharide molecular amount mainly is distributed between the 3000-300; (c) Radix Ginseng mixing oligosaccharide is made up of rhamnose, arabinose, xylose, galactose, glucose, glucuronic acid, galacturonic acid.(d) Radix Ginseng mixing oligosaccharide is made up of 2-7 sugar and is mixed oligosaccharide.
3. has the new purposes of anti-senile dementia, analgesia and anti-gastric-ulcer according to the described Radix Ginseng mixing of claim 2 oligosaccharide
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102827256A (en) * | 2012-09-25 | 2012-12-19 | 姜瑞芝 | Preparation and application of ginseng glycoprotein |
| CN102872178A (en) * | 2012-09-15 | 2013-01-16 | 吉林农业大学 | Preparation method of ginseng oligosaccharide extract and application of ginseng oligosaccharide extract |
| CN103385888A (en) * | 2013-07-24 | 2013-11-13 | 刘淑莹 | Application of ginseng oligosaccharide composition in preparation of anti-oxidation medicine or health-care food |
| CN105699478A (en) * | 2016-03-21 | 2016-06-22 | 长春中医药大学 | Method for quickly identifying sugar |
| CN106974952A (en) * | 2017-04-10 | 2017-07-25 | 通化鑫业生物科技研发有限公司 | Application of the fresh ginseng activity extract in treatment oral cavity and digestive tract ulcer medicine is prepared |
| CN112245342A (en) * | 2020-09-10 | 2021-01-22 | 孙立伟 | Ginseng series skin care extract and preparation method and application thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1049221C (en) * | 1995-10-30 | 2000-02-09 | 中国科学院昆明植物研究所 | Ginseng oligosaccharide element and ginseng monomer oligosaccharide and its preparing technology and use |
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2009
- 2009-02-24 CN CN2009100665625A patent/CN101502546B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102872178A (en) * | 2012-09-15 | 2013-01-16 | 吉林农业大学 | Preparation method of ginseng oligosaccharide extract and application of ginseng oligosaccharide extract |
| CN102827256A (en) * | 2012-09-25 | 2012-12-19 | 姜瑞芝 | Preparation and application of ginseng glycoprotein |
| CN103385888A (en) * | 2013-07-24 | 2013-11-13 | 刘淑莹 | Application of ginseng oligosaccharide composition in preparation of anti-oxidation medicine or health-care food |
| CN105699478A (en) * | 2016-03-21 | 2016-06-22 | 长春中医药大学 | Method for quickly identifying sugar |
| CN106974952A (en) * | 2017-04-10 | 2017-07-25 | 通化鑫业生物科技研发有限公司 | Application of the fresh ginseng activity extract in treatment oral cavity and digestive tract ulcer medicine is prepared |
| CN112245342A (en) * | 2020-09-10 | 2021-01-22 | 孙立伟 | Ginseng series skin care extract and preparation method and application thereof |
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| CN101502546B (en) | 2012-02-08 |
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