CN101497846A - Method for extracting Calophyllum essential oil constituent - Google Patents
Method for extracting Calophyllum essential oil constituent Download PDFInfo
- Publication number
- CN101497846A CN101497846A CNA2008100743691A CN200810074369A CN101497846A CN 101497846 A CN101497846 A CN 101497846A CN A2008100743691 A CNA2008100743691 A CN A2008100743691A CN 200810074369 A CN200810074369 A CN 200810074369A CN 101497846 A CN101497846 A CN 101497846A
- Authority
- CN
- China
- Prior art keywords
- kalofium
- root
- calophyllum inophyllum
- essential oil
- supercutical fluid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to an extraction method of calophyllum inophyllum linn essential oil components, which comprises the following steps: 1. drying calophyllum inophyllum linn fruits at low temperature, and crushing the calophyllum inophyllum linn fruits to be powder; 2. placing the calophyllum inophyllum linn powder in an extraction kettle, adding 0.1-0.3 time of entrainer ethanol by weight, setting the outlet flow of CO2 of supercutical fluid to be 10-50mL/min under the conditions of 25-40MPa of pressure and 35-60 DEGC of temperature, extracting the power added with the entrainer ethanol for 120 min-210 min, collecting and obtaining the extract of the calophyllum inophyllum linn supercutical fluid CO2; 3. dissolving the extract of the calophyllum inophyllum linn supercutical fluid CO2 into the ethanol solution with the weight being 5-10 times of that of the calophyllum inophyllum linn supercutical fluid CO2, and depressurizing and steaming to obtain the calophyllum inophyllum linn essential oil containing coumarin, genocide, stearic acid and ester. The invention has simple operation and high extraction ratio (which can reach 0.2-0.48 percent), and can be used for developing high-value product of perfume, medical health products, and the like because the extracted calophyllum inophyllum linn essential oil mainly contains the components of the coumarin, the genocide, the stearic acid, the ester, and the like.
Description
Technical field
The present invention relates to the extracting method of Calophyllum essential oil constituent, particularly a kind of employing supercritical CO
2The method that extraction process extracts.
Background technology
Root of Kalofium (Calophyllum inophyllum Linn), have another name called Malus spectabilis, poon, pittosporum tobira, gentleman tree, Calophyllum, fine jade state Malus spectabilis, be herba fibraureae recisae section (Guttiferae) Calophylum lanigerum (Calophyllum genus) aiphyllium, be distributed in provinces and regions such as Hainan, Guangdong, Guangxi, Taiwan in China, torrid areas such as South East Asia, South Asia, Africa all have.The root of Kalofium plant has very high pharmaceutical use, sanitas, antimicrobial drug, astringent, expectorant, hydragog(ue) and the cathartic often done among the people.The fruit and the seed of root of Kalofium are rich in grease, and kind oil real and root can control wound and scabies, intramuscular injection can end leprosy pain, and extract can be used for treating leprosy nephritis, also capable of resisting AIDS.Therefore the research that utilizes different extracting method that extraction, separation, the structure of effective Chemical Composition of root of Kalofium plant are identified has great importance and vast potential for future development.
Have the report that extraction, separation, structure about effective Chemical Composition of root of Kalofium plant are identified at present, as: Chen Ji army of Kunming plant institute of the Chinese Academy of Sciences etc. (chemical ingredients of the southern regions of the Yunnan Province root of Kalofium. Yunnan plant research, 2001, (23) report 4:521-526), from this plant, separate having obtained 5 compounds, comprising composition in calophylloide and the tonka bean camphor; (slender lobule root of Kalofium phyllody composition Study chemistry of forest product and industry such as Chen Guangying, 2003, (23) report 2:73-76) utilizes silica gel column chromatography to separate and the thin layer purifying, partly separates obtaining 6 compounds from the ethyl acetate of the ethanol extraction of Hainan slender lobule root of Kalofium leaf, determine that they are respectively suberone, canophyllol, different canophyllic acid, Tridemethylsciadopitysin, canophyllic acid, wherein different canophyllic acid find that in pharmacological evaluation liver cancer cell and lung carcinoma cell are had selecting cell toxicity.
But at present the report that the essential oil of root of Kalofium fruit is extracted is less, and the essential oil of root of Kalofium fruit contains compositions such as coumarins, xanthene ketone, hard ester acids, ester class, canophyllol, can be used for developing high-value products such as spices, medical health product.
Summary of the invention
The object of the invention is to provide a kind of extracting method of Calophyllum essential oil constituent, and it uses the supercritical CO 2 extraction process, has optimized the extraction process of Calophyllum essential oil constituent, improve extraction yield, and whole process has advantages such as safe, nontoxic, quick.
The present invention includes following steps:
1, with the cryodrying of root of Kalofium fruit, is ground into powder;
2, getting the root of Kalofium powder and place extraction kettle, add the entrainment agent ethanol of 0.1～0.3 times of weight, is 25MPa～40MPa at pressure, and temperature is under 35 ℃～60 ℃ the condition, to set supercutical fluid CO
2Rate of discharge be 10mL/min～50mL/min, extraction 120min～210min collects and to obtain root of Kalofium supercutical fluid CO
2Extract;
3, with root of Kalofium supercutical fluid CO
2Extract is dissolved in the ethanolic soln of 5～10 times of weight, and evaporated under reduced pressure is mainly contained the root of Kalofium essential oil of coumarins, xanthene ketone, hard ester acids, ester class composition again.
Beneficial effect of the present invention is: use traditional supercritical CO
2The extraction yield of the essential oil composition of extraction process extraction root of Kalofium fruit can reach 0.2%～0.48%, contains compositions such as coumarins, xanthene ketone, hard ester acids, ester class in the essential oil, can be used for developing high-value products such as spices, medical health product.
Embodiment
The present invention is described in further detail below by embodiments of the invention.But protection of the present invention does not mean that and only is confined to following examples:
Embodiment one:
1, with the cryodrying of root of Kalofium fruit, is ground into powder;
2, the root of Kalofium fruit 500g that gets pulverizing puts into extractor, with supercritical CO
2Feed in it, adding entrainment agent alcoholic acid volume is 50mL, and the setting extracting pressure is 25MPa, and temperature is 35 ℃, sets CO
2Rate of discharge be 10mL/min, pick up counting when reaching condition, the extraction time is 120min, collects to obtain root of Kalofium supercutical fluid CO
2Extract 2 grams;
3, with root of Kalofium supercutical fluid CO
2Extract 2 grams are dissolved in the 20mL ethanolic soln, and evaporated under reduced pressure obtains Calophyllum essential oil constituent 1 gram again.
Embodiment two:
1, with the cryodrying of root of Kalofium fruit, is ground into powder;
2, the root of Kalofium fruit 500g that gets pulverizing puts into extractor, with supercritical CO
2Feed in it, adding entrainment agent alcoholic acid volume is 150mL, and the setting extracting pressure is 40MPa, and temperature is 60 ℃, sets CO
2Rate of discharge be 50mL/min, pick up counting when reaching condition, the extraction time is 210min, collects to obtain root of Kalofium supercutical fluid CO
2Extract 5 grams;
3, with root of Kalofium supercutical fluid CO
2Extract 5 grams are dissolved in the 50mL ethanolic soln, and evaporated under reduced pressure obtains Calophyllum essential oil constituent 2.05 grams again.
Embodiment three:
1, with the cryodrying of root of Kalofium fruit, is ground into powder;
2, the root of Kalofium fruit 500g that gets pulverizing puts into extractor, with supercritical CO
2Feed in it, adding entrainment agent alcoholic acid volume is 100mL, and the setting extracting pressure is 35MPa, and temperature is 45 ℃, sets CO
2Rate of discharge be 20mL/min, pick up counting when reaching condition, the extraction time is 150min, collects to obtain root of Kalofium supercutical fluid CO
2Extract 4 grams;
3, with root of Kalofium supercutical fluid CO
2Extract 4 grams are dissolved in the 35mL ethanolic soln, and evaporated under reduced pressure obtains Calophyllum essential oil constituent 2.4 grams again.
Claims (1)
1, the extracting method of Calophyllum essential oil constituent is characterized in that this method may further comprise the steps:
(1) with the cryodrying of root of Kalofium fruit, is ground into powder;
(2) getting the root of Kalofium powder and place extraction kettle, add the entrainment agent ethanol of 0.1～0.3 times of weight, is 25MPa～40MPa at pressure, and temperature is under 35 ℃～60 ℃ the condition, to set supercutical fluid CO
2Rate of discharge be 10mL/min～50mL/min, extraction 120min～210min collects and to obtain root of Kalofium supercutical fluid CO
2Extract;
(3) with root of Kalofium supercutical fluid CO
2Extract is dissolved in the ethanolic soln of 5～10 times of weight, and evaporated under reduced pressure is mainly contained the root of Kalofium essential oil of coumarins, xanthene ketone, hard ester acids, ester class composition again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100743691A CN101497846A (en) | 2008-01-31 | 2008-01-31 | Method for extracting Calophyllum essential oil constituent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100743691A CN101497846A (en) | 2008-01-31 | 2008-01-31 | Method for extracting Calophyllum essential oil constituent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101497846A true CN101497846A (en) | 2009-08-05 |
Family
ID=40945118
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100743691A Pending CN101497846A (en) | 2008-01-31 | 2008-01-31 | Method for extracting Calophyllum essential oil constituent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101497846A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226131A (en) * | 2011-05-26 | 2011-10-26 | 华南理工大学 | Preparation method of myrtle essential oil and application of myrtle essential oil in Guangdong sausage |
| CN102071095B (en) * | 2009-08-27 | 2012-08-29 | 海南省粮油科学研究所 | Cold pressing preparation process for calophyllum inophyllum linn seed oil |
| CN109609275A (en) * | 2019-01-04 | 2019-04-12 | 湖北瑞晟生物有限责任公司 | A kind of extraction process of Rosemary Oil |
-
2008
- 2008-01-31 CN CNA2008100743691A patent/CN101497846A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071095B (en) * | 2009-08-27 | 2012-08-29 | 海南省粮油科学研究所 | Cold pressing preparation process for calophyllum inophyllum linn seed oil |
| CN102226131A (en) * | 2011-05-26 | 2011-10-26 | 华南理工大学 | Preparation method of myrtle essential oil and application of myrtle essential oil in Guangdong sausage |
| CN102226131B (en) * | 2011-05-26 | 2013-08-21 | 华南理工大学 | Preparation method of myrtle essential oil and application of myrtle essential oil in Guangdong sausage |
| CN109609275A (en) * | 2019-01-04 | 2019-04-12 | 湖北瑞晟生物有限责任公司 | A kind of extraction process of Rosemary Oil |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107022417B (en) | Method for extracting and separating effective components of leaf flowers by supercritical carbon dioxide | |
| CN1935820A (en) | Method for preparing total saponin of sapindusmukerossi | |
| CN110862869A (en) | By using supercritical CO2Method for preparing folium artemisiae argyi essential oil and byproducts thereof by extraction and secondary molecular distillation processes | |
| CN104906153A (en) | Technological method for efficiently extracting ginkgo flavone | |
| CN105132172B (en) | A method of preparing tobacco orrisroot Flavonoid substances from orrisroot | |
| CN101433592B (en) | Preparation method of Lupulus extract containing xanthohumol | |
| CN102965198A (en) | Method for extracting and processing lavender essential oil | |
| CN105342892B (en) | A kind of Rocket ram jet procyanidine creme and preparation method thereof | |
| RU2435766C1 (en) | Method of producing dihydroquercetin | |
| CN103169727B (en) | General-flavonoid compound in chionanthus as well as preparation method and application thereof | |
| CN109438213B (en) | Isopentenyl chalcone compound and preparation method thereof | |
| CN105503786A (en) | Secolignan compound-nettle secolignan glucoside E and preparation method thereof | |
| CN101497846A (en) | Method for extracting Calophyllum essential oil constituent | |
| CN102040612A (en) | Method for extracting bergamottin from grapefruit peel | |
| CN102731592A (en) | Method for extracting cleupin and amentoflavone from olive leaf | |
| CN103463160A (en) | Preparation method of high-content total flavones of chrysanthemum | |
| CN103585424B (en) | A kind of method extracting total flavones from leek seed | |
| CN103146473A (en) | Method for simultaneously extracting Australian tea tree essential oil and flavonoid glycosides | |
| CN106699819B (en) | The preparation method of Penta-O-galloyl-D-glucopyranose chemical reference substance | |
| CN105348333B (en) | A kind of chavicol disaccharide glycoside compound and its preparation method and application | |
| CN105412167A (en) | Method for extracting and separating ginkgo flavone and ginkgolide from ginkgo leaves | |
| CN103340917A (en) | Extraction method for flavonoids in mugwort | |
| CN114634537A (en) | Preparation method and application of diterpene in gold leaves | |
| CN101084985B (en) | separation and identification of highly effective antibiotic anti-oxidant active part of glycyrrhiza | |
| CN102241656A (en) | Preparation method of tricin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090805 |