CN101497779B - Mixed liquor for labeling and labeling method for animals - Google Patents
Mixed liquor for labeling and labeling method for animals Download PDFInfo
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- CN101497779B CN101497779B CN2009101112658A CN200910111265A CN101497779B CN 101497779 B CN101497779 B CN 101497779B CN 2009101112658 A CN2009101112658 A CN 2009101112658A CN 200910111265 A CN200910111265 A CN 200910111265A CN 101497779 B CN101497779 B CN 101497779B
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- labeling
- indicator
- magnetic
- mixed liquor
- sign
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to mixture indicator and an animal indicating method, belongs to an indicating method, and provides mixture indicator and an animal indicating method adopting the mixture indicator. Raw materials of the mixture indicator comprise magnetic particle and edible oil, wherein the magnetic particle at least contains iron and carbon. The mixture indicator is injected into an object to be indicated, a detector determines whether the object is indicated or not, and the indicated object is replaced after the indication is completed. Because the raw materials of the mixture indicator have simple component, stable chemical property, easy obtaining of the raw materials, low cost and easy preparation, the injection quantity of the mixture indicator is little, the indicating speed is very high, and the indicating cost is very low. The invention not only influences the indicated object little, but also has higher safety to a human body, has wide range of application, especially the application to living microbe, such as the cultivation of bionts of rabbit fish, amphioxus, loach, bird, and the like, and can be also used as an indicator of a nonmagnetic product (the product without influencing magnetic lines).
Description
Technical field
The present invention relates to a kind of identification method, especially relate to the identification method of a kind of mixed liquor for labeling and animal.
Background technology
Animal identification to zoologize ecology, migrate, migrate, breeding etc. is significant.Aspect animal identification, best identification method should satisfy following requirement: the marking materials physicochemical property are stable, little, as far as possible little to the animal normal physiological activity to the animal infringement, can satisfy the low-cost sign needs of scale in enormous quantities if relating to the human consumption marking materials can not have any injury to human body, can preserve for a long time and be identified easily after identifying.For the sign of small-sized organism is the comparison difficulty always, because sign damages organism, influences its normal physiological activity, even jeopardizes by the life of marker and survive.
Aspect globe fish; Globe fish can not excrete poison as snake; TTX is mainly derived from the biologic chain accumulation; Wild filefish is not huge with breed filefish toxicity intensity difference, and it is nontoxic to culture filefish (Fugurubripes, dark line globefish, yellowfin globefish etc.), and it is great to the edible inclusive sense of having ready conditions therefore to distinguish breed filefish and wild filefish.Effective sign to culturing filefish is very necessary.
Breed the field of releasing in fishery, identification method commonly used has external marks method, inner mark method.
The external marks method comprises that the notation of listing, fluorescent marker method or liquid rubber latex go into black labelling method, cut the fin notation, the labeling notation.The inner mark method comprises metal wire code labeling method, visual implantation notation, signal telemetry notation etc.
List the marker of notation outside by the sign organism, influence biont (such as the fish of releasing) normal activity, mortality ratio height becomes the target of similar cannibalisation easily.
Fluorescent marker method is to inject discernible fluorescent colloid in the appearance of hydrocoles, can have two kinds of colloids to mix the back and inject in the body, through 24 hours after coagulations, adopts photodiode, can observe fluorescent effect at night.Its shortcoming is that the infusion fluorescent glue exists the hidden danger on the edible safety, because injection volume must be in the naked eyes recognizable set, if be difficult for distinguishing influence sign effect because of skin color changes by marker in addition.
It is bigger to the injury of fish to cut the fin notation, and the labeling notation is fit to shellfish.
The metal wire code labeling of using always in the world in the internal indicator method is manufactured and designed by U.S. northwest oceanology company, is widely used in the seed hatching, cultures contrast, open-air biological marker etc.Its principle is that the magneticmetal line is injected by marker, confirms back proof sign through surveying.Advantage is can metal wire be encoded, and also can identify in enormous quantities.Shortcoming is that the magnetic thing maybe be to being impacted by marker; Instrument cost is too high, introduces about 1,500,000 yuan of a metal line code sign appearance Renminbi, 60,000 yuan of recognizers according to pertinent data (the Fujian aquatic products first phase in March, 2008).
The additive method of internal indicator is not because the cost height is used on a large scale yet.In addition, general sign need accurately not confirming individuality.
Notification number is the animal two-dimensional code ear tag that the utility model patent of CN201081915 provides a kind of embedding that electronic mark is arranged, and in use causes two dimension code reading inaccurate even the appearance of phenomenon such as can not normally distinguish owing to factor such as wearing and tearing, pollution and recognition condition are harsh to overcome existing animal two-dimensional code ear tag.This ear tag comprises the two-dimensional code ear tag, and said two-dimensional code ear tag comprises ear tag substrate and ear tag fastener, it is characterized in that: be provided with electronic mark in the said ear tag substrate housing.
Publication number is that the application for a patent for invention of CN1832679 discloses a kind of system that is used for animal identification.This system comprises: animal identification equipment, be suitable for being applied to animal and equipment, and equipment, be used for the organ data of safety storing from treating to extract by the animal of this marking equipment sign.Each all is marked with common identifier this storing device and this marking equipment.Before using, it can be in the supporter of moulded tray form that this marking equipment and storing device are installed in.
Notification number is the syringe that the utility model patent of CN2702718 discloses a kind of implanted electronic mark; Comprise body, push rod, cap, locator card; Characteristics are to be arranged with handle on the body and to be fixed with a syringe needle; Push rod is located in the body, an end can penetrate syringe needle, and cap sleeve is at the body front end, and locator card is located at the push rod root.During use, take down cap, the electronic mark of record animal or pet information is put into from syringe needle, take away locator card, find animal injection position, promote push rod, electronic mark is expelled under the animal skin, realize all-the-way tracking management this animal or pet.
(Zhou Yongdong such as Zhou Yongdong; Xu Hanxiang, Dai Xiaojie, Xu Kaida. the effect [J] of several kinds of labelling methodes in fishing resources propagation is released. the Fujian aquatic products; 2008,3:6-11) reported at home and abroad fishing resources propagation release in normally used several kinds of labelling methodes.
Summary of the invention
The object of the present invention is to provide a kind of mixed liquor for labeling.
Another object of the present invention is to provide a kind of identification method that adopts the animal of mixed liquor for labeling.
Its raw material of mixed liquor for labeling according to the invention consists of magnetic and edible oil.
The proportional quantity of magnetic and edible oil can be adjusted according to actual needs, presses mass ratio, and general magnetic: edible oil is 1: 1; The composition of said magnetic contains iron and carbon at least; Preferably the content of iron by mass percentage at least 70%, the HK-10 type black particle produced of Jiangsu Yixing City magnetic flux inspection material ltd for example, by mass percentage; The content of iron and carbon is carbon 0.64%, iron 98.12%, and surplus is trace element; The median size of magnetic is preferably less than 10 microns.
A kind of identification method that adopts the animal of said mixed liquor for labeling, its concrete steps are:
The mixed liquor for labeling injection is treated in the marker, determined whether to be identified, after the completion sign, will be put back to by marker with detector.
Said mixed liquor for labeling is injected treats preferably to adopt in the marker syringe.Said mixed liquor for labeling is injected treats preferably to inject the specified injection position of treating marker in the marker, treats that the injection position of marker treats that according to difference marker is selected, and for example fish can be selected positions such as pectoral fin root, head be subcutaneous as the injection position.
Said syringe can adopt plastics disposable sterilized injector or syringe pump as required; The two-tube syringe pump of LSP2-1B type that for example adopts Baoding LanGe constant flow pump Co., Ltd's (sino-america joint-venture) to produce, perhaps the KDS100 type micro-injection pump of Shenzhen Rui Wode Science and Technology Ltd. and KDS200 type micro-injection pump.When injection quantity is little, can select plastics disposable sterilized injector commonly used for use, for example the disposable sterilized injector of Shandong Xinhua Ande Medical Articles Co., Ltd.'s production.
Determine whether to be identified to adopt to send with detector and pipe or/and the method for glittering PL.
Said detector can adopt magnetic detection equipment, and for example the portable needle checking machine of 21EHERO-TY type produced of Dongguan promise ancient cooking vessel electronics technology ltd also can be other suitable detectors of sensitivity, for example 21EHERO-ENA platform machines etc.
Said mixed liquor for labeling is injected treats in the marker that when using syringe pump, controlled 0.05～10 μ l/ that is made as of the IR of mixed liquor for labeling goes on foot; When adopting the 1ml manual syringe, the minimum injection volume of may command is about 10 μ l/ time (being the half-space scale); By this calculating, the sign cost be about 0.003～0.005 yuan/inferior.Sign speed aspect is designated example with filefish, and its sign speed can reach that 1000～1200 tails/everyone per hour.If the non-living body article, its sign speed can reach higher.
Compare with other identification methods because that the raw material of the mixed liquor for labeling that the present invention adopts is formed is simple, chemical property is stable, raw material is easy to get is inexpensive, preparation easily, the mixed liquor for labeling IR is very small, sign speed is very high, it is very low therefore to identify cost.Owing to adopt the mixed liquor for labeling that is mixed by magnetic and edible oil as marker, its mixed liquor for labeling IR is very small again, and is therefore not only to atomic by the influence of marker, higher to the security of human body; And application is very extensive, especially its advantage is more showed in the application of living body biological, for example cultures bionts such as filefish, lancelet, loach, birds.Also can be used as simultaneously the sign of non-magnetic product (not influencing the product of magneticline of force).
The present invention has following outstanding advantage:
1) not only have advantages such as injection needles is little, the mixed liquor for labeling ID is small, disguise is stronger, and can independently select to identify the position, batch, quantity, flexibly initiatively, simple to operate, increase greatly and copy difficulty.
2) mixed liquor for labeling does not influence edible to human non-toxic, safety.
3) mixed liquor for labeling does not influence by the sign biological growth minimum by sign biological nontoxic, safety, harm, the recovery normal physiological activity that can be exceedingly fast after the sign, as ingest, grow, breeding etc.
4) sign efficient is high, cost is extremely low, is fit to the scale prodn requirement.
5) effective constituent of marker can forever be stored in by in the sign object, and physicochemical property is stable.
6) can 100% be verified recognition of devices by marker, and marking equipment and inspection machine are cheap, are not used the person easily and accept.
7) the present invention also can be used for general sign (non-magnetic induction thing) and purposes such as similar article (non magnetic inductor) differentiation.
Embodiment
The sign of embodiment 1 fish (is example to culture filefish)
1) magnetic is mixed (press mass ratio, magnetic: edible oil is 1: 1) with edible oil, stir, get mixed liquor for labeling;
2) suck mixed liquor for labeling with syringe, mixed liquor for labeling is injected treat marker breed filefish pectoral fin base portion, determine whether to be identified, accomplish sign, will culture filefish and put back to detector.
Press mass ratio, magnetic: edible oil is 1: 1, injects and treats marker--culture filefish body superficial place, (reducing the influence to filefish) can be selected the pectoral fin root or positions such as head is subcutaneous, dorsal fin base portion as far as possible.Increase magnetic amount can reduce can discern injection volume.
When adopting the manual injection, the once minimum injection volume of may command is about 10 μ l; By this calculating, the identification liquid cost is about 0.0035 yuan/inferior sign.When adopting micro-injection appearance (pump) injection, the once minimum injection volume of may command can be low to moderate 0.0919 μ l (60mlBD syringe).In view of lower injection volume still can be identified, cost still can reduce.The visible material cost almost can be ignored.
By the check once more before edible of sign filefish, what send that sign pipes signal then is the safe filefish of culturing, and not sending what pipe then is doubtful poisonous filefish.
To the tracking of the fish of releasing, can find migration route accurately.
The sign of embodiment 2 birds (is example with the homing pigeon)
Similar with embodiment 1, its difference is that injection position is at homing pigeon shank or sole.Compare with the method for listing, the present invention can alleviate the flight resistance of homing pigeon.
The identification method of embodiment 3 Amphibianss (is example to free frog)
Similar with embodiment 1, its difference is that injection position can be chosen in the frog leg muscle.
Embodiment 4 mammiferous signs (with the sheep is example, and new variety are herded sheep growth contrast, can assess the upgrowth situation of new variety under similarity condition)
Similar with embodiment 1, its difference is that injection position can be chosen in sheep bait edge or scalp.
Below only provide 4 embodiment, but given method all is applicable to other animal.
Below provide method principle of the present invention: magnetic thing (Z 250, red oxide of iron) can be magnetized; And can influence inspection machine (detection instrument) through cutting magnetic line; Select granularity extremely low (nano level) magnetic for use; Be mixed and made into the sign mixture with the oiliness thing, by marker specific position (can be different positions), realize permanent recognition purpose through injection system.(for example under injection live body globefish skin or after the muscle tissue, through rejection marker is formed coating, marker can not disperse.) magnetic carrying magnetic not itself, but very easily be magnetized, when magnetizer (detector) leaves, the remanent magnetism rate is 0, promptly recovers no magnetic state, so can not cause effect of magnetic influence.
Claims (1)
1. mixed liquor for labeling is characterized in that its raw material consists of magnetic and edible oil; Press mass ratio, magnetic: edible oil is 1: 1; The composition of said magnetic contains iron and carbon at least, the content of iron by mass percentage at least 70%, and the median size of magnetic is less than 10 microns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101112658A CN101497779B (en) | 2009-03-13 | 2009-03-13 | Mixed liquor for labeling and labeling method for animals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101112658A CN101497779B (en) | 2009-03-13 | 2009-03-13 | Mixed liquor for labeling and labeling method for animals |
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| Publication Number | Publication Date |
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| CN101497779A CN101497779A (en) | 2009-08-05 |
| CN101497779B true CN101497779B (en) | 2012-07-18 |
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| CN2009101112658A Expired - Fee Related CN101497779B (en) | 2009-03-13 | 2009-03-13 | Mixed liquor for labeling and labeling method for animals |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101703021B (en) * | 2009-11-25 | 2012-12-19 | 大连富谷水产有限公司 | Labeling method and labeling liquid for globefish trace amount |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1831079A (en) * | 2006-04-20 | 2006-09-13 | 上海交通大学 | Fluorescent, magnetic, multi-functional nanometer material and its prepn. method |
| CN101092250A (en) * | 2006-06-20 | 2007-12-26 | 中国科学院兰州化学物理研究所 | Method for preparing magnetic Nano material |
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2009
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1831079A (en) * | 2006-04-20 | 2006-09-13 | 上海交通大学 | Fluorescent, magnetic, multi-functional nanometer material and its prepn. method |
| CN101092250A (en) * | 2006-06-20 | 2007-12-26 | 中国科学院兰州化学物理研究所 | Method for preparing magnetic Nano material |
Non-Patent Citations (1)
| Title |
|---|
| 林元华.海洋生物标志放流技术的研究现状.《海洋科学》.1985,第9卷(第5期),54-58. * |
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| CN101497779A (en) | 2009-08-05 |
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Granted publication date: 20120718 Termination date: 20130313 |