CN101485364B - Method for producing edible lipid containing docosahexenoic acid by lipase-catalyzed ester exchange - Google Patents
Method for producing edible lipid containing docosahexenoic acid by lipase-catalyzed ester exchange Download PDFInfo
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- CN101485364B CN101485364B CN 200910060722 CN200910060722A CN101485364B CN 101485364 B CN101485364 B CN 101485364B CN 200910060722 CN200910060722 CN 200910060722 CN 200910060722 A CN200910060722 A CN 200910060722A CN 101485364 B CN101485364 B CN 101485364B
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Abstract
The invention provides a method for producing edible fat containing docosahexenoic acid by enzyme process and ester exchange. The method comprises the following steps: (1) putting DHA fat into the edible fat to add enzyme; (2) vacuumizing and heating a closed container to be stirred; and (3) charging nitrogen into the closed container after a reaction is completed, filtering to obtain the edible fat after ester exchange, obtaining finished product fat after molecular distillation, or removing non-esterified fatty acid after alkali refining, and deodorizing to obtain the finished product fat. The method has the advantages that the edible fat and the docosahexenoic acid (DHA for short) fat are catalyzed to carry out ester exchange by 1,3-selective immobilized lipase so as to produce the edible vegetable fat with rich docosahexenoic acid (short for DHA) and with high added value; the edible fat with rich docosahexenoic acid (short for DHA) is sealed in a proper packaging container, after the container is vacuumized and charged with nitrogen, the container is placed at room temperature for 18 to 24 months, the peroxide number of the edible fat is stilled maintained at less than or equal to 10meq/kg, and the oxidative stabilization of the fat is obviously improved.
Description
Technical field
The present invention relates to the polyunsaturated fatty acid production technical field, specifically use 1,3 optionally fixed lipase catalyzed edible oil and fat and DHA (being called for short DHA) grease carry out ester exchange, produce a kind of production method of the edible vegetable oil and fat of the high added value that is rich in DHA (being called for short DHA).
Background technology
In recent years, polyunsaturated fatty acid (Polyunsaturated fatty acids, PUFAs) more and more is subject to people's attention the important function of human health.Wherein, DHA is because of extremely people's the favor of its important physiological function in human body and animal body.DHA belongs to highly undersaturated aliphatic acid, very easily oxidative rancidity.The oxidation of DHA at first is exactly to cause content, and its effect is affected; Secondly, the product of oxidation is harmful to health, and takes in the body to cause lipid peroxidation, brings out various physically different and cause the generation of disease; In addition, oxidation product also can with food in proteins react, also can cause the oxidation of other oxygen sensitive composition in the food, thereby nutritive value of food and shelf life are affected.The oxidation of DHA also is the major reason that produces off-flavors.
Traditional handicraft is directly to be added in the edible oil and fat with DHA (be called for short DHA), and mix and blend adds antioxidant, carries out physical mixed and reaches the oxidation of control DHA, improves its stability.
Summary of the invention
The objective of the invention is to develop a kind of usefulness 1,3 optionally fixed lipase catalyzed edible oil and fat and DHA (being called for short DHA) grease carry out ester exchange, the edible vegetable oil and fat of the high added value of DHA (being called for short DHA) is rich in production, makes the production method of the edible oil and fat oxidation stability enhancing of adding DHA (being called for short DHA).
The present invention utilizes enzyme process to change composition or aliphatic acid a kind of triglycerides that the position prepares on glycerol backbone of triglycerides, changes the oxidation stability that the position distribution situation of DHA in triglycerides improves edible oil and fat by the Structured Lipids technology.
The method of the edible oil and fat that contain DHA is produced in enzyme process ester exchange of the present invention, and its step is as follows:
(1) in closed container, with the DHA grease in hybrid reaction after the ratio of required DHA content put in the edible oil and fat, enzyme-added by the 4-7% of total weight of oil percentage, DHA content is 1-50% in the DHA grease of described interpolation;
(2) closed container vacuumizes, and vacuum is-0.08～-0.1MPa, be warming up to 60-75 ℃, stir, stir speed (S.S.) is 50-300 rev/min, about time 3-6 hour;
(3) reaction is complete, fills nitrogen in closed container, filters, and obtains the edible oil and fat after the ester exchange, obtains product oil or with traditional alkali refining method removal free fatty, deodorization obtains product oil behind molecular clock.
Edible oil and fat include but not limited to palm oil, soybean salad oil, sunflower oil, tea oil, olive oil, siritch, peanut oil.
The advantage that the method for the edible oil and fat that contain DHA is produced in enzyme process ester exchange of the present invention is: with 1,3 optionally fixed lipase catalyzed edible oil and fat and DHA (being called for short DHA) grease carry out ester exchange, the edible vegetable oil and fat of the high added value of DHA (being called for short DHA) is rich in production, the edible oil and fat that are rich in DHA (being called for short DHA) are sealed in the proper packing container, placed 18 to 24 months in room temperature behind the vacuum nitrogen filling, peroxide value still remains on≤10meq/kg, and its oxidation stability significantly strengthens.
The specific embodiment
Embodiment one:
The method of the edible oil and fat that contain DHA is produced in a kind of enzyme process ester exchange, and its step is as follows:
(1) in closed container, after getting 0.4kgDHA grease and 100kg sunflower oil and mixing, add the 4kg enzyme, DHA content is 1% in the DHA grease of described interpolation;
(2) closed container vacuumizes, and vacuum is-0.08MPa, is warming up to 60 ℃, stirs, and stir speed (S.S.) is 50 rev/mins, 3 hours time;
(3) reaction is complete, fills nitrogen in closed container, is cooled to 50 ℃, filters, and obtains the sunflower oil after the ester exchange, obtains DHA content and be 0.004% product oil 96kg behind molecular clock.
Embodiment two:
The method of the edible oil and fat that contain DHA is produced in a kind of enzyme process ester exchange, and its step is as follows:
(1) in closed container, get 0.002kgDHA grease and 100kg mixed with olive oil after, add the 7kg enzyme, DHA content is 50% in the DHA grease of described interpolation;
(2) closed container vacuumizes, and vacuum is-0.1MPa, is warming up to 75 ℃, stirs, and stir speed (S.S.) is 300 rev/mins, 6 hours time;
(3) reaction is complete, fills nitrogen in closed container, is cooled to 60 ℃, filters, and obtains the palm oil after the ester exchange, removes free fatty behind alkali refining, and it is 0.001% product oil 93kg that deodorization obtains DHA content.
Embodiment three:
The method of the edible oil and fat that contain DHA is produced in a kind of enzyme process ester exchange, and its step is as follows:
(1) in closed container, after getting 0.008kgDHA grease and 100kg tea oil and mixing, add the 5.5kg enzyme, DHA content is 27% in the DHA grease of described interpolation;
(2) closed container vacuumizes, and vacuum is-0.09MPa, is warming up to 67 ℃, stirs, and stir speed (S.S.) is 175 rev/mins, 4.5 hours time;
(3) reaction is complete, fills nitrogen in closed container, is cooled to 55 ℃, filters, and obtains the tea oil after the ester exchange, obtains DHA content and be 0.002% product oil 94.5kg behind molecular clock.
Embodiment four:
The method of the edible oil and fat that contain DHA is produced in a kind of enzyme process ester exchange, and its step is as follows:
(1) 100kgDHA grease and 25kg palm oil are dropped in the closed container, add the 5kg enzyme, DHA content is 20% in the DHA grease of described interpolation;
(2) closed container vacuumizes, and vacuum is-0.08MPa, is warming up to 60 ℃, stirs, and stir speed (S.S.) is 50 rev/mins, 3 hours time;
(3) reaction is complete, fills nitrogen in closed container, is cooled to 50 ℃, filters, and obtains the DHA grease after the ester exchange, removes free fatty behind molecular clock, obtains DHA content and be 16% finished product DHA grease 121kg.
Embodiment five
The method of the edible oil and fat that contain DHA is produced in a kind of enzyme process ester exchange, and its step is as follows:
(1) 140kg DHA grease and 20kg olive oil are dropped in the closed container, add the 11.2kg enzyme, DHA content is 50% in the DHA grease of described interpolation;
(2) closed container vacuumizes, and vacuum is-0.1MPa, is warming up to 75 ℃, stirs, and stir speed (S.S.) is 300 rev/mins, 6 hours time;
(3) reaction is complete, fills nitrogen in closed container, is cooled to 60 ℃, filters, and obtains the DHA grease after the ester exchange, and with traditional alkali refining method removal free fatty, it is 43.75% finished product grease 152kg that deodorization obtains DHA content.
Embodiment six:
The method of the edible oil and fat that contain DHA is produced in a kind of enzyme process ester exchange, and its step is as follows:
(1) 110kg DHA grease and 20kg tea oil are dropped in the closed container, add the 7.15kg enzyme, DHA content is 35% in the DHA grease of described interpolation;
(2) closed container vacuumizes, and vacuum is-0.09MPa, is warming up to 67 ℃, stirs, and stir speed (S.S.) is 175 rev/mins, 4.5 hours time;
(3) reaction is complete, fills nitrogen in closed container, is cooled to 55 ℃, filters, and obtains the DHA grease after the ester exchange, removes free fatty behind molecular clock, and obtaining DHA content is 29.62% finished product grease 125kg.
In addition to the implementation, the present invention can form a plurality of embodiment, does not give unnecessary details one by one at this.
Claims (1)
1. the method for the edible oil and fat that contain DHA is produced in an enzyme process ester exchange, it is characterized in that its step is as follows:
(1) in closed container, with the DHA grease in hybrid reaction after the ratio of required DHA content put in the edible oil and fat, enzyme-added by the 4-7% of total weight of oil percentage, DHA content is 1-50% in the DHA grease of described interpolation;
(2) closed container vacuumizes, and vacuum is-0.08～-0.1MPa, be warming up to 60-75 ℃, stir, stir speed (S.S.) is 50-300 rev/min, time 3-6 hour;
(3) reaction is complete, fills nitrogen in closed container, filters, and obtains the edible oil and fat after the ester exchange, and through obtaining removing free fatty behind product oil or the alkali refining behind the molecular clock, deodorization obtains product oil,
Wherein, the edible oil and fat that contain DHA that obtain are sealed in the proper packing container, placed 18 to 24 months in room temperature behind the vacuum nitrogen filling, peroxide value still remains on≤10meq/kg, and wherein said edible oil and fat are selected from palm oil, soybean salad oil, sunflower oil, tea oil, olive oil, siritch, peanut oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910060722 CN101485364B (en) | 2009-02-11 | 2009-02-11 | Method for producing edible lipid containing docosahexenoic acid by lipase-catalyzed ester exchange |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910060722 CN101485364B (en) | 2009-02-11 | 2009-02-11 | Method for producing edible lipid containing docosahexenoic acid by lipase-catalyzed ester exchange |
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| CN101485364A CN101485364A (en) | 2009-07-22 |
| CN101485364B true CN101485364B (en) | 2013-05-01 |
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| CN 200910060722 Expired - Fee Related CN101485364B (en) | 2009-02-11 | 2009-02-11 | Method for producing edible lipid containing docosahexenoic acid by lipase-catalyzed ester exchange |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255949A (en) * | 2014-09-19 | 2015-01-07 | 广东润科生物工程有限公司 | Peanut blend oil containing docosahexaenoic acid (DHA), and preparation method thereof |
| CN104255952A (en) * | 2014-09-19 | 2015-01-07 | 广东润科生物工程有限公司 | DHA-containing corn blend oil and preparation method thereof |
| CN107372864A (en) * | 2016-05-17 | 2017-11-24 | 嘉必优生物技术（武汉）股份有限公司 | Composition, preparation method and application containing DHA grease |
| CN109247397A (en) * | 2018-09-18 | 2019-01-22 | 湖北福星生物科技有限公司 | A kind of Sn-2 Structure grease and preparation method thereof rich in docosahexaenoic acid |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1816614A (en) * | 2003-07-09 | 2006-08-09 | 日清奥利友集团株式会社 | Process for producing symmetrical triglyceride |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1816614A (en) * | 2003-07-09 | 2006-08-09 | 日清奥利友集团株式会社 | Process for producing symmetrical triglyceride |
Non-Patent Citations (1)
| Title |
|---|
| Kuan-hsiang Huang et al.Lipase-Catalyzed Incorporation of n-3 Polyunsaturated Fatty Acids into Vegetable Oils.《JAOCS》.1994,第71卷(第11期),1277-1280. * |
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Owner name: ROQUETTE BIOLOGICAL NUTRIMENT (WUHAN) CO., LTD. Free format text: FORMER OWNER: YOUZHIYOU BIOLOGICAL SCI-TECH CO., LTD., HUBEI Effective date: 20100908 |
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