CN101484462A - 通过定向进化产生催化优化的嵌合锌指重组酶 - Google Patents

通过定向进化产生催化优化的嵌合锌指重组酶 Download PDF

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CN101484462A
CN101484462A CNA2007800254608A CN200780025460A CN101484462A CN 101484462 A CN101484462 A CN 101484462A CN A2007800254608 A CNA2007800254608 A CN A2007800254608A CN 200780025460 A CN200780025460 A CN 200780025460A CN 101484462 A CN101484462 A CN 101484462A
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seq
recombinase
sequence
chimeric
site
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卡洛斯·F·巴巴斯三世
拉塞尔·M·高德雷
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Scripps Research Institute
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Scripps Research Institute
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

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  • General Chemical & Material Sciences (AREA)
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  • Physical Education & Sports Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CNA2007800254608A 2006-07-05 2007-07-05 通过定向进化产生催化优化的嵌合锌指重组酶 Pending CN101484462A (zh)

Applications Claiming Priority (2)

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US81890806P 2006-07-05 2006-07-05
US60/818,908 2006-07-05

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CN101484462A true CN101484462A (zh) 2009-07-15

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US (1) US8685687B2 (https=)
EP (1) EP2046806A4 (https=)
JP (1) JP2009542228A (https=)
KR (1) KR20090031938A (https=)
CN (1) CN101484462A (https=)
AU (1) AU2007269048A1 (https=)
CA (1) CA2667974A1 (https=)
WO (1) WO2008006028A2 (https=)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104781404A (zh) * 2012-09-04 2015-07-15 斯克利普斯研究院 具有靶向结合特异性的嵌合多肽
CN104789555A (zh) * 2015-04-29 2015-07-22 江南大学 一种基于合成单链dna文库进化基因表达调控元件的方法
CN105814573A (zh) * 2013-09-27 2016-07-27 科德克希思公司 基于结构的预测性建模
CN106232827A (zh) * 2014-02-21 2016-12-14 哈佛学院董事及会员团体 变构蛋白的从头设计
CN108138164A (zh) * 2015-08-21 2018-06-08 孟山都技术公司 基因组基因座的增强的重组
US10696964B2 (en) 2013-09-27 2020-06-30 Codexis, Inc. Automated screening of enzyme variants

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CA2822302A1 (en) 2006-02-13 2007-08-23 Alethia Biotherapeutics Inc. Methods of impairing osteoclast differentiation
US8168181B2 (en) * 2006-02-13 2012-05-01 Alethia Biotherapeutics, Inc. Methods of impairing osteoclast differentiation using antibodies that bind siglec-15
MY160435A (en) * 2008-06-12 2017-03-15 Univ Putra Malaysia A novel antiviral peptide against avian influenza virus h9n2
WO2010042894A1 (en) * 2008-10-09 2010-04-15 The Regents Of The University Of California NOVEL NUCLEIC ACID CONSTRUCTS CONTAINING ORTHOGONAL SITE SELECTIVE RECOMBINASES (OSSRs)
GB2467167B (en) * 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
WO2011017315A2 (en) 2009-08-03 2011-02-10 Recombinetics, Inc. Methods and compositions for targeted gene modification
US20130005590A1 (en) 2011-06-06 2013-01-03 The Regents Of The University Of California Synthetic biology tools
WO2013163628A2 (en) * 2012-04-27 2013-10-31 Duke University Genetic correction of mutated genes
US9738879B2 (en) 2012-04-27 2017-08-22 Duke University Genetic correction of mutated genes
HK1210192A1 (en) 2012-07-19 2016-04-15 Alethia Biotherapeutics Inc. Anti-siglec-15 antibodies
US10793867B2 (en) * 2013-03-15 2020-10-06 Monsanto Technology, Llc Methods for targeted transgene-integration using custom site-specific DNA recombinases
US9828582B2 (en) 2013-03-19 2017-11-28 Duke University Compositions and methods for the induction and tuning of gene expression
WO2014197748A2 (en) 2013-06-05 2014-12-11 Duke University Rna-guided gene editing and gene regulation
JP6303195B2 (ja) * 2013-11-20 2018-04-04 国立研究開発法人産業技術総合研究所 細菌による機能的外来タンパク質の製造方法
US11319555B2 (en) 2014-11-20 2022-05-03 Duke University Compositions, systems and methods for cell therapy
WO2016130600A2 (en) 2015-02-09 2016-08-18 Duke University Compositions and methods for epigenome editing
WO2017035416A2 (en) 2015-08-25 2017-03-02 Duke University Compositions and methods of improving specificity in genomic engineering using rna-guided endonucleases
EP4089175A1 (en) 2015-10-13 2022-11-16 Duke University Genome engineering with type i crispr systems in eukaryotic cells
KR102787119B1 (ko) 2015-11-30 2025-03-27 듀크 유니버시티 유전자 편집에 의한 인간 디스트로핀 유전자의 교정을 위한 치료용 표적 및 사용 방법
CN106011162A (zh) * 2016-01-27 2016-10-12 浙江大学 大肠杆菌细胞内原位进化目标蛋白质的新方法
US20190127713A1 (en) 2016-04-13 2019-05-02 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
JP7490211B2 (ja) 2016-07-19 2024-05-27 デューク ユニバーシティ Ｃｐｆ１に基づくゲノム編集の治療適用
EP3717505A4 (en) 2017-12-01 2021-12-01 Encoded Therapeutics, Inc. GENENICALLY MODIFIED DNA-BINDING PROTEINS
EP3740580A4 (en) 2018-01-19 2021-10-20 Duke University GENOME ENGINEERING WITH CRISPR-CAS SYSTEMS IN EUKARYONTS
CN109979591B (zh) * 2019-03-12 2021-01-01 众安信息技术服务有限公司 一种基于图神经网络分析斑块进展因子的方法及装置
PL3976798T3 (pl) * 2019-05-29 2026-04-07 Encoded Therapeutics, Inc. Kompozycje i sposoby selektywnej regulacji genów
CN114729376A (zh) 2019-09-23 2022-07-08 欧米茄治疗公司 用于调节肝细胞核因子4α(HNF4α)基因表达的组合物和方法
CN110868598B (zh) * 2019-10-17 2021-06-22 上海交通大学 基于对抗生成网络的视频内容替换方法及系统
US20210130828A1 (en) * 2019-11-01 2021-05-06 Sangamo Therapeutics, Inc. Gin recombinase variants
CN121693564A (zh) 2023-06-26 2026-03-17 夏威夷大学 进化的整合酶和其用于基因组编辑的方法

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CN1303930A (zh) * 1999-10-27 2001-07-18 上海博容基因开发有限公司 一种新的多肽——锌指蛋白57和编码这种多肽的多核苷酸
WO2005107790A1 (en) * 2004-04-30 2005-11-17 Poetic Genetics, Llc Hybrid recombinases for genome manipulation

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GB0222229D0 (en) * 2002-09-25 2002-10-30 Univ Glasgow Mutant recombinases
US7470777B2 (en) * 2004-12-22 2008-12-30 Iowa State University Research Foundation, Inc. Compositions and methods related to modified retroviral vectors for restricted, site specific integration

Patent Citations (2)

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CN1303930A (zh) * 1999-10-27 2001-07-18 上海博容基因开发有限公司 一种新的多肽——锌指蛋白57和编码这种多肽的多核苷酸
WO2005107790A1 (en) * 2004-04-30 2005-11-17 Poetic Genetics, Llc Hybrid recombinases for genome manipulation

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104781404A (zh) * 2012-09-04 2015-07-15 斯克利普斯研究院 具有靶向结合特异性的嵌合多肽
CN104781404B (zh) * 2012-09-04 2018-10-26 斯克利普斯研究院 具有靶向结合特异性的嵌合多肽
CN109517068A (zh) * 2012-09-04 2019-03-26 斯克利普斯研究院 具有靶向结合特异性的嵌合多肽
US10696964B2 (en) 2013-09-27 2020-06-30 Codexis, Inc. Automated screening of enzyme variants
US11535845B2 (en) 2013-09-27 2022-12-27 Codexis, Inc. Automated screening of enzyme variants
CN105814573A (zh) * 2013-09-27 2016-07-27 科德克希思公司 基于结构的预测性建模
US11342046B2 (en) 2013-09-27 2022-05-24 Codexis, Inc. Methods and systems for engineering biomolecules
CN106232827A (zh) * 2014-02-21 2016-12-14 哈佛学院董事及会员团体 变构蛋白的从头设计
US10920217B2 (en) 2014-02-21 2021-02-16 President And Fellows Of Harvard College De novo design of allosteric proteins
CN104789555A (zh) * 2015-04-29 2015-07-22 江南大学 一种基于合成单链dna文库进化基因表达调控元件的方法
CN108138164B (zh) * 2015-08-21 2022-05-10 孟山都技术公司 基因组基因座的增强的重组
CN108138164A (zh) * 2015-08-21 2018-06-08 孟山都技术公司 基因组基因座的增强的重组
US12460217B2 (en) 2015-08-21 2025-11-04 Monsanto Technology Llc Enhanced recombination of genomic loci

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WO2008006028A3 (en) 2008-12-24
WO2008006028A2 (en) 2008-01-10
JP2009542228A (ja) 2009-12-03
EP2046806A4 (en) 2009-10-21
US8685687B2 (en) 2014-04-01
KR20090031938A (ko) 2009-03-30
US20100086532A1 (en) 2010-04-08
AU2007269048A1 (en) 2008-01-10
EP2046806A2 (en) 2009-04-15
CA2667974A1 (en) 2008-01-10

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Application publication date: 20090715