CN101463094A - Preparation of Sphallerocerpus gracilis polysaccharide - Google Patents
Preparation of Sphallerocerpus gracilis polysaccharide Download PDFInfo
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- CN101463094A CN101463094A CN 200710307283 CN200710307283A CN101463094A CN 101463094 A CN101463094 A CN 101463094A CN 200710307283 CN200710307283 CN 200710307283 CN 200710307283 A CN200710307283 A CN 200710307283A CN 101463094 A CN101463094 A CN 101463094A
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- gracilis polysaccharide
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Abstract
The invention discloses a preparation method of polysaccharide from holothuria monacaria. The method comprises the following steps: the holothuria monacaria is crushed into 0.5cm grains, the grains are soaked in water, boiled and extracted, extracting solution is depressurized and condensed in vacuum, added with edible alcohol, kept standing for precipitation and centrifuged to remove supernatant, and a precipitate is collected and is subject to spray drying to obtain a polysaccharide extract from the holothuria monacaria. The yield of the obtained polysaccharide extract from the holothuria monacaria is 15-30% based on raw materials by the method, and the polysaccharide content of the extract is determined as 72-88% by a sulfuric acid-phenol method. The polysaccharide extract from the holothuria monacaria can be made into pills, tablets or capsules.
Description
Technical field
The invention belongs to the natural product chemistry research field, relate to natural product extraction, separation and authentication method, particularly a kind of preparation method of Sphallerocerpus gracilis polysaccharide.
Background technology
Yellow ginseng is the meat rhizome of samphire Sphallerocarpus gracilis (Sphallerocarpus gracilis), has another name called yellow turnip, the red sieve fore-telling in leaflet mountain.All there is distribution ground and Mongolia and east, USSR (Union of Soviet Socialist Republics) siberian and the Far East Area in China Heilungkiang, Jilin, Gansu, Xinjiang, Qinghai etc.Yellow ginseng meat rhizome is rich in trace elements such as multiple amino acids, mineral substance and P, Mg, K, Cu, Cr, Co, Zn, the Mn of polysaccharide and needed by human.Yellow ginseng is nontoxic direct-edible, is a kind of pure natural health-care, and wherein polysaccharide is its main nutrient composition.
Studies show that, polysaccharide can effectively strengthen human immune system's immunologic function, is a kind of effective immunostimulant, also have simultaneously bring high blood pressure down, the effect of blood sugar and cholesterol, and certain antitumor action is arranged, so thought good dietary supplements by international medical community.
Summary of the invention
The object of the present invention is to provide the low mass-producing Sphallerocerpus gracilis polysaccharide of a kind of extraction yield height, cost to extract the preparation method.
The present invention realizes by following measure:
A kind of preparation of Sphallerocerpus gracilis polysaccharide, after it is characterized in that this method is ground into the particle of 0.5cm size with the Huang ginseng, to boil extraction behind the water logging bubble, the reduced vacuum concentrated extracting solution, adding edible ethanol staticly settles, supernatant liquor is removed in centrifuging, and spraying drying promptly gets the Sphallerocerpus gracilis polysaccharide extract behind the collecting precipitation thing.
The present invention is technical scheme more specifically:
Preparation of Sphallerocerpus gracilis polysaccharide, carry out according to following four steps:
The first step: soaking boils carries
The Huang ginseng that clean dry is good is ground into the bulk of 0.5cm size, adds the water logging bubble of 6～10 times of amounts, and heating is boiled and carried for several times then, and each 2～4 hours, filter back united extraction liquid, discard the material slag; Extracting solution is evaporated to 1/10～1/20 of cumulative volume obtains concentrated solution;
Second step: alcohol precipitation
Under agitation condition, in step 1, add 95% edible ethanol in the concentrated solution, make that ethanol content reaches 60～80% in the solution, leave standstill to make to be settled out Sphallerocerpus gracilis polysaccharide;
The 3rd step: filter
After leaving standstill, supernatant liquor is slowly inclined to, lower floor's solution is filtered, collect solid sediment;
The 4th step: spraying drying
With the solids spraying drying that obtains, both can get the extract of Sphallerocerpus gracilis polysaccharide.
The Sphallerocerpus gracilis polysaccharide extract productive rate that obtains by the present invention is calculated as 15～30% by raw material, and polysaccharide content is 72～88% in the employing sulfuric acid-phynol method mensuration extract.
The dry matter of the Sphallerocerpus gracilis polysaccharide of processing acquisition can be made into pill, tablet or capsule according to the method described above.
The qualitative analysis of Sphallerocerpus gracilis polysaccharide:
1. dissolution experiment
In water, dehydrated alcohol, acetone, ether, ethyl acetate, add a small amount of Sphallerocerpus gracilis polysaccharide, stir the dissolving situation of observing.Sphallerocerpus gracilis polysaccharide is soluble in water, and its aqueous solution presents transparent thick.Be insoluble to organic solvents such as dehydrated alcohol, acetone, ether, ethyl acetate.
2. sulfuric acid-phynol reaction
Get 1% (g/v) Sphallerocerpus gracilis polysaccharide solution, add 5% phenol solution 1.0ml, vitriol oil 7.0ml leaves standstill 5min behind the shake well, then in 85 ℃ of heating in water bath 15min, observes solution and changes, and solution colour becomes orange, and the result is positive.
3. the quantitative analysis of Sphallerocerpus gracilis polysaccharide:
With glucose is the reference substance preparing standard solution, and the employing sulfuric acid-phynol method is measured the polysaccharide content in the Sphallerocerpus gracilis polysaccharide product that makes.
Accurate respectively standard solution, sample solution 0.6, the 2.0ml of drawing respectively places 10ml tool plug scale test tube.After adding water in the standard solution test tube and being supplemented to 2.0ml, add 5% phenol solution 1.0ml in each pipe, vitriol oil 7.0ml, shake well leaves standstill 5min, places 85 ℃ of water-baths to heat 15min.Take out, be cooled to room temperature.Prepare blank solution with distilled water with the method operation.Adopt spectrophotometry, the working sample solution absorbency, according to polysaccharide content in the following formula calculation sample:
In the formula: C is the content (μ g/ml) that records glucose in the sample solution, and D is the final dilution volume (ml) of sample solution, and m is sample quality (μ g).
Embodiment
Be embodiments of the invention below, but the present invention is not limited only to embodiment.
Embodiment 1:
Clean the drying of Huang ginseng with gathering is ground into the bulk of 0.5cm size.Take by weighing the Huang ginseng raw material that 1kg crushes, soak 0.5h behind the water of adding 8000mL, heating is boiled and is carried 2h then, extracts twice altogether, slightly cooling back gauze elimination residue.Be evaporated to 800～1500mL after twice extracting solution merged, slowly add 95% edible ethanol then while stirring, make that ethanol content reaches 60～80% in the solution, leave standstill 24h, the Sphallerocerpus gracilis polysaccharide precipitation is separated out.Slowly inclining the upper strata stillness of night, and decompression recycling ethanol filters lower floor's solution, collects the solid sediment spraying drying, promptly gets the Sphallerocerpus gracilis polysaccharide extract.Its Sphallerocerpus gracilis polysaccharide extract productive rate is calculated as 27% by raw material, and polysaccharide content is 75% in the employing sulfuric acid-phynol method mensuration extract.
Claims (2)
1, a kind of preparation of Sphallerocerpus gracilis polysaccharide, after it is characterized in that this method is ground into the particle of 0.5cm size with the Huang ginseng, to boil extraction behind the water logging bubble, the reduced vacuum concentrated extracting solution, adding edible ethanol staticly settles, supernatant liquor is removed in centrifuging, and spraying drying promptly gets the Sphallerocerpus gracilis polysaccharide extract behind the collecting precipitation thing.
2, the method for claim 1 is characterized in that this method carries out according to following four steps:
The first step: soaking boils carries
The Huang ginseng that clean dry is good is ground into the bulk of 0.5cm size, adds the water logging bubble of 6～10 times of amounts, and heating is boiled and carried for several times then, and each 2～4 hours, filter back united extraction liquid, discard the material slag; Extracting solution is evaporated to 1/10～1/20 of cumulative volume obtains concentrated solution;
Second step: alcohol precipitation
Under agitation condition, in step 1, add 95% edible ethanol in the concentrated solution, make that ethanol content reaches 60～80% in the solution, leave standstill to make to be settled out Sphallerocerpus gracilis polysaccharide;
The 3rd step: filter
After leaving standstill, supernatant liquor is slowly inclined to, lower floor's solution is filtered, collect solid sediment;
The 4th step: spraying drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200710307283 CN101463094A (en) | 2007-12-18 | 2007-12-18 | Preparation of Sphallerocerpus gracilis polysaccharide |
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| CN 200710307283 CN101463094A (en) | 2007-12-18 | 2007-12-18 | Preparation of Sphallerocerpus gracilis polysaccharide |
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| CN101463094A true CN101463094A (en) | 2009-06-24 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103524632A (en) * | 2013-03-21 | 2014-01-22 | 王喜军 | Rapid preparation process of astragalus polysaccharide |
| CN104072628A (en) * | 2014-06-10 | 2014-10-01 | 西北师范大学 | Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method |
| CN109721665A (en) * | 2019-03-15 | 2019-05-07 | 西北农林科技大学 | A kind of hot water extraction method of the Sphallerocerpus gracilis polysaccharide with high anti-oxidation ability |
| CN109721666A (en) * | 2019-03-15 | 2019-05-07 | 西北农林科技大学 | A kind of ultrasonic auxiliary extraction method of Sphallerocerpus gracilis polysaccharide |
| CN109820886A (en) * | 2019-04-01 | 2019-05-31 | 西北农林科技大学 | A kind of extraction of kurarinone cauline leaf polyphenol and isolation and purification method |
| CN113121718A (en) * | 2021-05-13 | 2021-07-16 | 东南大学 | Roselle polysaccharide PSGP-2 and preparation method and application thereof |
-
2007
- 2007-12-18 CN CN 200710307283 patent/CN101463094A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103524632A (en) * | 2013-03-21 | 2014-01-22 | 王喜军 | Rapid preparation process of astragalus polysaccharide |
| CN103524632B (en) * | 2013-03-21 | 2016-06-08 | 王喜军 | The quick reparation technology that a kind of astragalus polysaccharides produces |
| CN104072628A (en) * | 2014-06-10 | 2014-10-01 | 西北师范大学 | Method for extracting sphallerocarpus gracilis polysaccharide through complex enzyme method |
| CN109721665A (en) * | 2019-03-15 | 2019-05-07 | 西北农林科技大学 | A kind of hot water extraction method of the Sphallerocerpus gracilis polysaccharide with high anti-oxidation ability |
| CN109721666A (en) * | 2019-03-15 | 2019-05-07 | 西北农林科技大学 | A kind of ultrasonic auxiliary extraction method of Sphallerocerpus gracilis polysaccharide |
| CN109820886A (en) * | 2019-04-01 | 2019-05-31 | 西北农林科技大学 | A kind of extraction of kurarinone cauline leaf polyphenol and isolation and purification method |
| CN113121718A (en) * | 2021-05-13 | 2021-07-16 | 东南大学 | Roselle polysaccharide PSGP-2 and preparation method and application thereof |
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Open date: 20090624 |