CN101457155B - Method for microbial decalcification and co-production of hydrogen in crude oil - Google Patents
Method for microbial decalcification and co-production of hydrogen in crude oil Download PDFInfo
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- CN101457155B CN101457155B CN2007101792718A CN200710179271A CN101457155B CN 101457155 B CN101457155 B CN 101457155B CN 2007101792718 A CN2007101792718 A CN 2007101792718A CN 200710179271 A CN200710179271 A CN 200710179271A CN 101457155 B CN101457155 B CN 101457155B
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- 239000010779 crude oil Substances 0.000 title claims abstract description 46
- 230000000813 microbial effect Effects 0.000 title claims abstract description 36
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 239000001257 hydrogen Substances 0.000 title claims abstract description 30
- 229910052739 hydrogen Inorganic materials 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000004519 manufacturing process Methods 0.000 title abstract description 6
- 238000000855 fermentation Methods 0.000 claims abstract description 37
- 230000004151 fermentation Effects 0.000 claims abstract description 37
- 238000006243 chemical reaction Methods 0.000 claims abstract description 36
- 241000894006 Bacteria Species 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000002347 injection Methods 0.000 claims abstract description 23
- 239000007924 injection Substances 0.000 claims abstract description 23
- 239000008399 tap water Substances 0.000 claims abstract description 23
- 235000020679 tap water Nutrition 0.000 claims abstract description 23
- 244000005700 microbiome Species 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 239000007789 gas Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 30
- 230000007480 spreading Effects 0.000 claims description 14
- 238000003892 spreading Methods 0.000 claims description 14
- 235000013379 molasses Nutrition 0.000 claims description 13
- 239000002699 waste material Substances 0.000 claims description 13
- 230000001580 bacterial effect Effects 0.000 claims description 10
- 241000193755 Bacillus cereus Species 0.000 claims description 9
- 241000194108 Bacillus licheniformis Species 0.000 claims description 9
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims description 9
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 7
- 241000335053 Beta vulgaris Species 0.000 claims description 7
- 239000003921 oil Substances 0.000 claims description 7
- 235000015278 beef Nutrition 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- 150000003863 ammonium salts Chemical class 0.000 claims description 4
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 4
- 159000000003 magnesium salts Chemical class 0.000 claims description 4
- 239000011785 micronutrient Substances 0.000 claims description 4
- 235000013369 micronutrients Nutrition 0.000 claims description 4
- 229940045641 monobasic sodium phosphate Drugs 0.000 claims description 4
- 239000001103 potassium chloride Substances 0.000 claims description 4
- 235000011164 potassium chloride Nutrition 0.000 claims description 4
- 230000035484 reaction time Effects 0.000 claims description 4
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 4
- 159000000000 sodium salts Chemical class 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical group OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 2
- 244000063299 Bacillus subtilis Species 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical group [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- 229940061607 dibasic sodium phosphate Drugs 0.000 claims description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical group [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000002068 microbial inoculum Substances 0.000 claims description 2
- 238000005065 mining Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000002689 soil Substances 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 abstract description 3
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 230000008569 process Effects 0.000 description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 7
- 239000011575 calcium Substances 0.000 description 7
- 229910052791 calcium Inorganic materials 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 150000007524 organic acids Chemical class 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000007670 refining Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000011033 desalting Methods 0.000 description 2
- -1 organic acid salt Chemical class 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to a method for the combined production of hydrogen by crude oil microbial decalcification, which comprises the steps of carrying out amplification culture on various microbial strains by adopting a conventional microbial amplification culture mode, mixing the amplified strains and fermentation products according to any proportion, and enabling the concentration of the strains to be 108～1010Adding a microorganism mixed bacterium and a fermentation product which account for 1-10% of the weight of tap water or oilfield injection water and 1-6% of a culture medium into the tap water or oilfield injection water to prepare a microorganism fermentation liquid, adding the microorganism fermentation liquid and crude oil into a closed container according to the weight ratio of 0.1: 1-0.5: 1, carrying out full mixing reaction at the temperature of 25-55 ℃ for 1-5 days under the condition of no air, collecting gas generated in the reaction process, wherein the decalcification rate of the crude oil reaches 97.8%, and the yield of hydrogen reaches 1mol-1The purity of the hydrogen in the culture medium reaches 93.2 percent.
Description
Technical field
The present invention relates to a kind ofly in the crude oil upgrading process, utilize microbial fermentation solution to carry out the method for former oil refining co-producing hydrogen.
Background technology
Along with the continuous increase and the external heavy crude of a large amount of import of China's BO output, the metal content in the crude oil increases progressively gradually.The increase of metal content in the crude oil; Not only bring many disadvantageous effects,, cause the serious fouling of furnace tubing as the poisoning of catalyst deactivation phenomenom in secondary processing process, occurring to Crude Oil Processing; Influence the quiet run of crude production device; And can reduce the postorder quality product, and increase the crude oil tooling cost, reduce the processing benefit of crude oil.
CN1431276, CN1521239, CN1760338, CN1657594, CN1657593, CN1245201, CN1472282, CN1611571, CN1611570, CN1611569, CN1611567, CN1611568, CN1267707, CN1865403, CN1657596 have all described a kind of chemical method and have particularly removed the metal process in the crude oil with organic acid or mineral acid; It is two point defects below main the existence in practical application: the one, utilize the electric desalting system of oil refining apparatus; For assurance device reaches decalcification desalting effect preferably; Need to inject the excessive organic acid and the bigger water yield; Thereby cause the unreacted organic acid of part to remain in the crude oil; Cause the corrosion of distillation plant even postorder processing units, influence oil refining apparatus safety, smooth running; The 2nd, the organic acid consumption is big, makes crude oil demetalization cost increase, because the sewage of demetalization process discharging contains part organic acid or organic acid salt, makes COD content up to tens thousand of mg/L, directly impacts the industrial sewage treatment system.
CN1865404 discloses the biological demetallated method of a kind of hydrocarbon ils, and this method is only applicable to the crude oil demetalization, does not relate to the method that microbiological crude oil takes off while co-producing hydrogen in the demetalization process; CN1958618A discloses the biological decalcification method in a kind of process for preparing chitin, and what relate generally to is agricultural byproducts decalcification technical field; CN101007682, CN1918079, CN1858213, CN1858197, CN1850582, CN1772877, CN1618980, CN1506465, CN1328158, CN1276430 provide the relevant method of utilizing mikrobe to come hydrogen manufacturing respectively; But all do not relate to when utilizing mikrobe hydrogen manufacturing, also be used for removing the content of the calcium metal of crude oil.
Summary of the invention
The object of the invention be to provide a kind of utilize mikrobe and tunning thereof under the amphimicrobian condition to the crude oil effect; Make mikrobe in the growth metabolism process, produce acid, aerogenesis, product surfactant; When crude oil was carried out decalcification, the microbial fermentation of fermentation generation hydrogen carried out the method for crude oil demetalization co-producing hydrogen.
The present invention realizes through following measure: with each bacterial classification of required mikrobe; Adopt the conventional mikrobe mode of spreading cultivation to spread cultivation; Above-mentioned bacterium and tunning thereof after will spreading cultivation respectively mix by arbitrary proportion again, obtain mikrobe mixed bacterium and tunning thereof, and after making its bacterium index reach certain requirement; Add mentioned microorganism mixed bacterium and tunning thereof and substratum in tap water or the oilfield injection water to certain concentration then; Be mixed with microbial fermentation solution, microbial fermentation solution and the crude oil with preparation joins in the encloses container in proportion then, under certain temperature and obstructed air conditions, carries out the thorough mixing reaction; Keep the amphimicrobian state in the entire reaction course, collect the gas that reaction process produces simultaneously.After cultivating the reaction certain hour, the calcium contents of assay determination crude oil, hydrogen output and hydrogen purity.
Described microbe-derived in oil field mining liquid or oil field soil sample, be subtilis (Bacillus subtilis), its preserving number is 1.942; Bacillus cereus (Bacillus cereus); Its preserving number is 1.1626, Bacillus licheniformis (Bacillus licheniformis), and its preserving number is 1.807; Pseudomonas aeruginosa (Pseudomonas aeruginosa), its preserving number are one or more the bacterial classification in 1.922.Said bacterial classification all can be provided by China Committee for Culture Collection of Microorganisms common micro-organisms center, and this mikrobe mixed bacterium can be mixed by arbitrary proportion mutually.
The material of one or more that the said substratum that microorganism growth is provided is waste molasses, beef extract or inorganic salt micronutrient element.
Keep in the substratum of microorganism growth; The waste molasses add-on accounts for 1～6% of microbial fermentation solution weight; The beef extract add-on accounts for 0～0.5% of microbial fermentation solution weight; In the inorganic salt micronutrient element, the ammonium salt add-on account for microbial fermentation solution weight 0～0.5%, the sodium salt add-on account for microbial fermentation solution weight 0～0.5%, the sylvite add-on account for microbial fermentation solution weight 0～0.5%, the magnesium salts add-on accounts for 0～0.05% of microbial fermentation solution weight.
Said waste molasses is the beet waste molasses; Beef extract is that one or both combination of components in Carnis Bovis seu Bubali cream or the peptone form, and ammonium salt is an ammonium nitrate or ammonium sulfate, and sodium salt is Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC; Sylvite is potassium primary phosphate or potassium hydrogenphosphate, and magnesium salts is a sal epsom.
The method that spreads cultivation of said mikrobe mixed bacterium spreads cultivation according to the conventional training method of mikrobe, makes the concentration of microbial fermentation product reach 10
8～10
10Individual/the ml mixed bacterium, the pH value is 5～7, and surviving rate is 85～99%.
Said cultured mikrobe and tunning thereof account for tap water or oilfield injection water weight 1～10%, cultivation fiduciary point tap water that adds in cultured mikrobe and the tunning thereof or oilfield injection water weight 1～6%.
In the microbial fermentation solution of said preparation and the crude oil hybrid reaction process, the weight ratio of microbial fermentation solution and crude oil effect is 0.1: 1～0.5: 1, and operative temperature is 25～55 ℃, and cultivating the reaction times is 1～5 day.
In the microbial fermentation solution of said preparation and the crude oil hybrid reaction process, microbial inoculum and crude oil operative temperature are 30～45 ℃, and cultivating the reaction times is 2～3 days.
Compared with prior art, the present invention has following effect:
Disclosed crude oil decalcifying technology of prior art or process for making hydrogen are independent process; Technique effect is single, and the present invention utilizes mikrobe and tunning thereof, and crude oil decalcifying technology and co-producing hydrogen process are organically combined; Improved the economic benefit of biological decalcification technology largely; And the crude oil decalcifying rate is reached more than 85%, purity reaches more than 90%, has that technology is simple, energy consumption is low, the safety and environmental protection characteristics.
Embodiment
Below in conjunction with embodiment the present invention is made further detailed description.
Embodiment 1
Subtilis, bacillus cereus, Bacillus licheniformis and pseudomonas aeruginosa bacterial classification that experiment is required; Adopt the conventional mikrobe mode of spreading cultivation to spread cultivation; Above-mentioned bacterium after will spreading cultivation respectively again and tunning thereof were by weight 1: 1: 1: 1 mixes; Obtain mikrobe mixed bacterium and tunning thereof, and make its bacteria concentration reach 6.8 * 10
9Individual/ml, pH5.0, survival rate 90%; Then mentioned microorganism mixed bacterium and tunning thereof with account for tap water or oilfield injection water weight 10%, the beet waste molasses culture medium adds in tap water or the oilfield injection water with the concentration that accounts for tap water or oilfield injection water weight 6%; Be mixed with microbial fermentation solution, again microbial fermentation solution and crude oil added in the encloses container by weight 0.1: 1, under 25 ℃ of temperature of reaction and obstructed air conditions, carry out the thorough mixing reaction; Keep the amphimicrobian state in the entire reaction course; Collect the gas that reaction process produces simultaneously, through cultivating reaction 5 days, the calcium contents that records crude oil is reduced to 21 μ g/g by 248 μ g/g; The decalcification rate is 91.5%, and the output of hydrogen is 0.7mol.mol
-1Substratum, the purity of hydrogen are 91.2%.
Embodiment 2
Subtilis, bacillus cereus, Bacillus licheniformis and pseudomonas aeruginosa bacterial classification that experiment is required; Adopt the conventional mikrobe mode of spreading cultivation to spread cultivation; Above-mentioned bacterium after will spreading cultivation respectively again and tunning thereof were by weight 1: 0: 1: 0 mixes; Obtain mikrobe mixed bacterium and tunning thereof, and make its bacteria concentration reach 1 * 10
10Individual/ml, pH is 6.5, survival rate 95%; Then mentioned microorganism mixed bacterium and tunning thereof to account for tap water or oilfield injection water weight 5%, the beet waste molasses in the substratum, potassium hydrogenphosphate, sal epsom add in tap water or the oilfield injection water with the concentration that accounts for tap water or oilfield injection water weight 3%, 0.5% and 0.05% respectively, are mixed with microbial fermentation solution; Again microbial fermentation solution and crude oil were added in the encloses container by weight 0.5: 1; Under 40 ℃ of temperature of reaction and obstructed air conditions, carry out the thorough mixing reaction, keep the amphimicrobian state in the entire reaction course, collect the gas that reaction process produces simultaneously; Through cultivating reaction 1 day; The calcium contents that records crude oil is reduced to 19 μ g/g by 928 μ g/g, and the decalcification rate is 97.8%, and the output of hydrogen is 1mol.mol
-1Substratum, the purity of hydrogen are 90.4%.
Embodiment 3
Subtilis, bacillus cereus, Bacillus licheniformis and pseudomonas aeruginosa bacterial classification that experiment is required; Adopt the conventional mikrobe mode of spreading cultivation to spread cultivation; Above-mentioned bacterium after will spreading cultivation respectively again and tunning thereof were by weight 2: 1: 2: 1 mixes; Obtain mikrobe mixed bacterium and tunning thereof, and make its bacteria concentration reach 1.0 * 10
8Individual/ml, pH is 7.0, survival rate 99%; Then mentioned microorganism mixed bacterium and tunning thereof to account for tap water or oilfield injection water weight 1.7%; Beet waste molasses in the substratum, Carnis Bovis seu Bubali cream, potassium hydrogenphosphate, SODIUM PHOSPHATE, MONOBASIC, sal epsom add in tap water or the oilfield injection water with the concentration that accounts for tap water or oilfield injection water weight 1%, 0.5%, 0.5%, 0.5% and 0.05% respectively; Be mixed with microbial fermentation solution, again microbial fermentation solution and crude oil added in the encloses container by weight 0.3: 1, under 55 ℃ of temperature of reaction and obstructed air conditions, carry out the thorough mixing reaction; Keep the amphimicrobian state in the entire reaction course; Collect the gas that reaction process produces simultaneously, through cultivating reaction 3 days, the calcium contents of crude oil is reduced to 17 μ g/g by 164 μ g/g; The decalcification rate is 89.6%, and the output of hydrogen is 1mol.mol
-1Substratum, the purity of hydrogen are 92.6%.
Embodiment 4
Subtilis, bacillus cereus, Bacillus licheniformis and pseudomonas aeruginosa bacterial classification that experiment is required; Adopt the conventional mikrobe mode of spreading cultivation to spread cultivation; Above-mentioned bacterium after will spreading cultivation respectively again and tunning thereof were by weight 1: 0: 0: 0 mixes; Obtain mikrobe mixed bacterium and tunning thereof, and make its bacteria concentration reach 9.2 * 10
9Individual/ml, pH is 6.2, survival rate 85%; Then mentioned microorganism mixed bacterium and tunning thereof to account for tap water or oilfield injection water weight 2.0%, the beet waste molasses in the substratum accounts for the concentration of tap water or oilfield injection water weight 1% to be added in tap water or the oilfield injection water, is mixed with microbial fermentation solution; Again microbial fermentation solution and crude oil were added in the encloses container by weight 0.3: 1; Under 40 ℃ of temperature of reaction and obstructed air conditions, carry out the thorough mixing reaction, keep the amphimicrobian state in the entire reaction course, collect the gas that reaction process produces simultaneously; Through cultivating reaction 5 days; The calcium contents of crude oil is reduced to 25 μ g/g by 196 μ g/g, and the decalcification rate is 87.2%, and the output of hydrogen is 0.9mol.mol
-1Substratum, the purity of hydrogen are 93.2%.
Embodiment 5
Subtilis, bacillus cereus, Bacillus licheniformis and pseudomonas aeruginosa bacterial classification that experiment is required; Adopt the conventional mikrobe mode of spreading cultivation to spread cultivation; Above-mentioned bacterium after will spreading cultivation respectively again and tunning thereof were by weight 0: 1: 1: 1 mixes; Obtain mikrobe mixed bacterium and tunning thereof, and make its bacteria concentration reach 6.2 * 10
9Individual/ml, pH is 5.2, survival rate 95%; Then mentioned microorganism mixed bacterium and tunning thereof to account for tap water or oilfield injection water weight 3.5%; Beet waste molasses in the substratum, peptone, potassium hydrogenphosphate, SODIUM PHOSPHATE, MONOBASIC, sal epsom add in tap water or the oilfield injection water with the concentration that accounts for tap water or oilfield injection water weight 2%, 0.5%, 0.5%, 0.5% and 0.05% respectively; Be mixed with microbial fermentation solution, again microbial fermentation solution and crude oil added in the encloses container by weight 0.3: 1, under 30 ℃ of temperature of reaction and obstructed air conditions, carry out the thorough mixing reaction; Keep the amphimicrobian state in the entire reaction course; Collect the gas that reaction process produces simultaneously, through cultivating reaction 3 days, the calcium contents of crude oil is reduced to 23 μ g/g by 296 μ g/g; The decalcification rate is 92.2%, and the output of hydrogen is 0.6mol.mol
-1Substratum, the purity of hydrogen are 90.4%.
Claims (5)
1. the method for a crude oil mikrobe decalcification co-producing hydrogen; It is characterized in that: with each bacterial classification of required mikrobe; Adopt the conventional mikrobe mode of spreading cultivation to spread cultivation, above-mentioned bacterium and tunning thereof after will spreading cultivation respectively mix by arbitrary proportion again, and making its bacterium index reach concentration is 10
8～10
10Individual/the ml mixed bacterium, the pH value is 5～7, and surviving rate is 85～99%; Add mentioned microorganism mixed bacterium and tunning thereof in tap water or the oilfield injection water to cultivation fiduciary point tap water or oilfield injection water weight 1～6% amount by accounting for tap water or oilfield injection water weight 1～10% then; Be mixed with microbial fermentation solution, microbial fermentation solution and crude oil join in the encloses container by weight the ratio that is 0.1: 1～0.5: 1, are 25～55 ℃ in temperature; Cultivating the reaction times is 1～5 day; Carry out the thorough mixing reaction under the obstructed air conditions, keep the amphimicrobian state in the entire reaction course, collect the gas that reaction process produces;
Described microbe-derived in oil field mining liquid or oil field soil sample; Be one or more the mikrobe associate strain in subtilis (Bacillus subtilis), bacillus cereus (Bacillus cereus), Bacillus licheniformis (Bacillus licheniformis) or the pseudomonas aeruginosa (Pseudomonas aeruginosa), said bacterial classification provides by China Committee for Culture Collection of Microorganisms common micro-organisms center.
2. the method for crude oil mikrobe decalcification co-producing hydrogen according to claim 1 is characterized in that: microbial inoculum and crude oil operative temperature are 30～45 ℃, and cultivating the reaction times is 2～3 days.
3. the method for crude oil mikrobe decalcification co-producing hydrogen according to claim 1 is characterized in that: substratum is one or more a material of waste molasses, beef extract or inorganic salt micronutrient element.
4. the method for crude oil mikrobe decalcification co-producing hydrogen according to claim 3; It is characterized in that: the waste molasses add-on accounts for 1～6% of microbial fermentation solution weight in the substratum; The beef extract add-on accounts for 0～0.5% of microbial fermentation solution weight; In the inorganic salt micronutrient element, the ammonium salt add-on account for microbial fermentation solution weight 0～0.5%, the sodium salt add-on account for microbial fermentation solution weight 0～0.5%, the sylvite add-on account for microbial fermentation solution weight 0～0.5%, the magnesium salts add-on accounts for 0～0.05% of microbial fermentation solution weight.
5. the method for crude oil mikrobe decalcification co-producing hydrogen according to claim 4; It is characterized in that: waste molasses is the beet waste molasses; Beef extract is that one or both combination of components in Carnis Bovis seu Bubali cream or the peptone form, and ammonium salt is an ammonium nitrate or ammonium sulfate, and sodium salt is Sodium phosphate, dibasic or SODIUM PHOSPHATE, MONOBASIC; Sylvite is potassium primary phosphate or potassium hydrogenphosphate, and magnesium salts is a sal epsom.
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| CN1865404A (en) * | 2005-05-20 | 2006-11-22 | 中国石油天然气股份有限公司 | Hydrocarbon oil biological decalcification method |
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| CN1865404A (en) * | 2005-05-20 | 2006-11-22 | 中国石油天然气股份有限公司 | Hydrocarbon oil biological decalcification method |
Non-Patent Citations (1)
| Title |
|---|
| 于娟等.微生物脱除高钙原油中钙的研究.《新疆石油天然气》.2007,第3卷(第2期), * |
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