Summary of the invention
The object of the present invention is to provide a kind of Chinese medicinal granule that contains Radix Notoginseng extract.
Another object of the present invention is to provide a kind of preparation method that contains the Chinese medicinal granule of Radix Notoginseng extract.
The Chinese medicinal granule that the present invention contains Radix Notoginseng extract is achieved through the following technical solutions:
Made by Chinese medicine extract and pharmaceutically acceptable carrier, wherein Chinese medicine extract accounts for 40~90% of percentage by weight, and pharmaceutically acceptable vehicle weight percentage composition is 10~60%; Described Chinese medicine extract is made up of following materials of weight proportions: Radix Salviae Miltiorrhizae extract 5.0%~80.0%, Radix Notoginseng extract 15.0%~93.0% and Borneolum Syntheticum or Lignum Dalbergiae Odoriferae oil 2.0%~15.0%; Content of danshinolic acid B is at 45%-70% in the above-mentioned Radix Salviae Miltiorrhizae extract, and salvianolic acid E content is at 2-10%, and rosmarinic acid contents is at 4%-20%, and alkannic acid content is at 1%-10%, and its total phenolic content is more than 70%; Arasaponin R1 content is 2%-10% in the Radix Notoginseng extract, ginsenoside Re's content is 2%-6%, and ginsenoside Rg1's content is 15%-40%, and ginsenoside Rb1's content is 15%-40%, ginsenoside Rd's content is 5%-12%, and the content of its Radix Notoginseng total arasaponins is more than 70%.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, preferably is achieved through the following technical solutions:
Weight percentage by Chinese medicine extract is 70~80%, and pharmaceutically acceptable vehicle weight percentage composition is 20~30%; Wherein said Chinese medicine extract is made up of following materials of weight proportions: Radix Salviae Miltiorrhizae extract 25.0%~50.0%, Radix Notoginseng extract 40.0%~65.0% and Borneolum Syntheticum or Lignum Dalbergiae Odoriferae oil 4.0%~10.0%; Content of danshinolic acid B is at 45%-70% in the above-mentioned Radix Salviae Miltiorrhizae extract, and salvianolic acid E content is at 2-10%, and rosmarinic acid contents is at 4%-20%, and alkannic acid content is at 1%-10%, and its total phenolic content is more than 70%; Arasaponin R1 content is 2%-10% in the Radix Notoginseng extract, ginsenoside Re's content is 2%-6%, and ginsenoside Rg1's content is 15%-40%, and ginsenoside Rb1's content is 15%-40%, ginsenoside Rd's content is 5%-12%, and the content of its Radix Notoginseng total arasaponins is more than 70%.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, and the best is achieved through the following technical solutions:
Weight percentage by Chinese medicine extract is 70~80%, and pharmaceutically acceptable vehicle weight percentage composition is 20~30%; Wherein Chinese medicine extract is made up of following materials of weight proportions: Radix Salviae Miltiorrhizae extract 30.3%, Radix Notoginseng extract 60.6% and Borneolum Syntheticum or Lignum Dalbergiae Odoriferae oil 9.1%; Content of danshinolic acid B is at 45%-70% in the above-mentioned Radix Salviae Miltiorrhizae extract, and salvianolic acid E content is at 2-10%, and rosmarinic acid contents is at 4%-20%, and alkannic acid content is at 1%-10%, and its total phenolic content is more than 70%; Arasaponin R1 content is 2%-10% in the Radix Notoginseng extract, ginsenoside Re's content is 2%-6%, and ginsenoside Rg1's content is 15%-40%, and ginsenoside Rb1's content is 15%-40%, ginsenoside Rd's content is 5%-12%, and the content of its Radix Notoginseng total arasaponins is more than 70%.
Chinese medicine extract described in the present invention, can utilize the preparation method of prior art to obtain, for example can utilize Chinese patent application CN1352985A, CN1247855A, CN1242364A, CN1384090A, 02117923.9, (Yunnan University of Traditional Chinese Medicine's journal such as Guo Ying, 2001,24 (4): preparation method 6) obtains.Also can grope preparation technology voluntarily obtains.Content of danshinolic acid B is at 45%-70% in the Radix Salviae Miltiorrhizae extract of the present invention, and salvianolic acid E content is at 2-10%, and rosmarinic acid contents is at 4%-20%, and alkannic acid content is at 1%-10%, and its total phenolic content is preferably in more than 80% more than 70%.No matter be by prior art or groping preparation technology voluntarily prepares Radix Salviae Miltiorrhizae extract of the present invention, if do not reach above-mentioned content standard, then should make with extra care, make it to meet above-mentioned content standard.Its assay and finger printing are as follows:
(1) assay (high performance liquid chromatography) of salvianolic acid B, salvianolic acid E, rosmarinic acid, alkannic acid in the above-mentioned Radix Salviae Miltiorrhizae extract
Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; Acetonitrile-water-phosphoric acid (23.5: 76.5: 0.02) is mobile phase; The detection wavelength is 288nm.Number of theoretical plate is pressed the salvianolic acid B peak and is calculated, and should be not less than 5000.
The preparation precision of reference substance solution takes by weighing the salvianolic acid B reference substance, adds mobile phase and makes the solution that every 1ml contains 0.2mg; Salvianolic acid E is made the solution that every 1ml contains 0.02mg; Rosmarinic acid is made the solution that every 1ml contains 0.05mg; Alkannic acid is made the solution that every 1ml contains 0.01mg.
The preparation precision of need testing solution takes by weighing the about 35mg of this product, puts in the 25ml measuring bottle, adds the mobile phase dissolving and is diluted to scale, shakes up; Precision is measured 5ml and is put in the 25ml measuring bottle, is diluted to scale with mobile phase, shakes up, namely.
Algoscopy is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, injects chromatograph of liquid, measures, namely.
(2) mensuration (spectrophotography) of the total phenolic acid of above-mentioned Radix Salviae Miltiorrhizae extract
The preparation precision of reference substance solution takes by weighing the salvianolic acid B reference substance, makes the solution that every 1ml contains 20 μ g with acetonitrile-water-phosphoric acid (23.5: 76.5: 0.02) mixed solution, namely.
The preparation precision of need testing solution takes by weighing the about 25mg of this product, puts in the 50ml measuring bottle, with acetonitrile-water-phosphoric acid (23.5: 76.5: 0.02) mixed solution dissolving and be diluted to scale, shake up, precision is measured 2ml, puts in the 50ml measuring bottle, add above-mentioned mixed solution and be diluted to scale, shake up, namely.
Algoscopy is got reference substance solution and need testing solution respectively, is blank with acetonitrile-water-phosphoric acid (23.5: 76.5: 0.02), according to spectrophotography (1995 editions appendix VA of Chinese Pharmacopoeia), measures trap at 288nm wavelength place, is calculated as follows, namely.
Total phenolic content (%)=f (A-B)+B
In the formula: f is correction factor 0.626;
A is that spectrophotometry is the content of total phenolic acid of contrast calculating with the salvianolic acid B;
B is the content of the salvianolic acid B of high effective liquid chromatography for measuring.
(3) above-mentioned Radix Salviae Miltiorrhizae extract HPLC finger printing
Assay method is referring to the assay (high performance liquid chromatography) of salvianolic acid B, salvianolic acid E, rosmarinic acid, alkannic acid in (1) above-mentioned Radix Salviae Miltiorrhizae extract.The record chromatograph time is 60 minutes.
Bigger and the metastable total peak of peak area salvianolic acid B is basic calculation relative retention time and relative peak area as the reference peak with the reference peak in the total fingerprint peaks of employing.The finger printing of above-mentioned Radix Salviae Miltiorrhizae extract should have 5-7 total peak, is generally 6 total peaks.The relative retention time at 6 total peaks is followed successively by 0.55-0.65 (salvianolic acid E peak), 0.66-0.70 (rosmarinic acid peak), 0.71-0.79 (alkannic acid peak), 1 (salvianolic acid B), 1.03-1.12,1.21-1.30.In the total peak unimodal area account for total peak area greater than 20% have only salvianolic acid B (namely with reference to the peak), salvianolic acid B peak area (namely with reference to the peak) accounts for the 57%-87% of total peak area, its relative peak area is 1; Relative retention time is that total peak (the being the rosmarinic acid peak) peak area of 0.66-0.70 accounts for the 3%-18% of total peak area, and its relative peak area is 0.03-0.25.The non-total peak gross area is not more than 10% of total peak area.
Radix Notoginseng extract in the above-mentioned Chinese medicine composition, can utilize the preparation method of prior art to obtain, for example can utilize (foreign medical science plant amedica fascicles such as Chinese patent ZL1095363C, Chinese patent application CN1352985A, Qian Tianxiang, 1997,5), the preparation method of national ministry standard WS3-B-3590-2001 (Z) obtains Radix Notoginseng extract 12 (4)), Tang's light (Chinese patent medicine 1990,12 (8):.Also can grope preparation technology voluntarily and extract Radix Notoginseng extract.Can also directly buy Radix Notoginseng extract from the market, for example content is the Radix Notoginseng total arasaponins (wherein Rb1 〉=30%, Rg1 〉=20%, R1 〉=5%, HPLC measure) of 95% (UV mensuration).Arasaponin R1 content should be 2%-10% in the Radix Notoginseng extract of the present invention, ginsenoside Re's content should be 2%-6%, ginsenoside Rg1's content should be 15%-40%, ginsenoside Rb1's content should be 15%-40%, ginsenoside Rd's content should be 5%-12%, the content of its Radix Notoginseng total arasaponins should be preferably in more than 80% more than 70%.No matter be to buy by prior art for preparing or market, do not reach above-mentioned content standard as purity, then should make with extra care, make it to meet above-mentioned content standard.Its assay and finger printing are as follows:
(1) ginsenoside Re, ginsenoside Rd, arasaponin R1, ginsenoside Rg1, ginsenoside Rb1's assay (high performance liquid chromatography) in the above-mentioned Radix Notoginseng extract
Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; Column temperature: 40 ℃, flow velocity: 0.7ml/min, the detection wavelength is 203nm; The mobile phase of gradient elution is as follows:
Time |
Water |
The second eyeball |
0 |
70 |
30 |
10 |
70 |
30 |
30 |
10 |
90 |
The preparation precision of reference substance solution takes by weighing reference substance, add methanol and make the solution that every 1ml contains the 0.2mg ginsenoside Re respectively, every 1ml contains 0.4mg ginsenoside Rd's solution, every 1ml contains the solution of 0.2mg ginsenoside R1, every 1ml contains the solution of 0.4mg Panax Notoginseng saponin R g1, and every 1ml contains 0.4mg ginsenoside Rb1's solution.
The preparation precision of need testing solution takes by weighing the about 20mg of this product, puts in the 50ml measuring bottle, adds the mobile phase dissolving and is diluted to scale, shakes up, namely.
Algoscopy is accurate reference substance solution and each 10 μ l of need testing solution of drawing respectively, injects chromatograph of liquid, measures, namely.Radix Notoginseng extract HPLC finger printing of the present invention.
(2) mensuration of above-mentioned Radix Notoginseng extract total saponins (spectrophotography)
(3) above-mentioned Radix Notoginseng extract HPLC finger printing
Assay method is referring to the assay (high performance liquid chromatography) of ginsenoside Re, ginsenoside Rd, arasaponin R1, ginsenoside Rg1, ginsenoside Rb1 in (1) above-mentioned Radix Notoginseng extract.The record chromatograph time is 30 minutes.
Bigger and the metastable total peak ginsenoside Rg1 of peak area is basic calculation relative retention time and relative peak area as the reference peak with the reference peak in the total fingerprint peaks of employing.The finger printing of above-mentioned Radix Notoginseng extract should have 9-12 total peak, is generally 11 total peaks.The relative retention time at 11 total peaks is followed successively by 0.77-0.85 (arasaponin R1 peak), (0.87-0.97 ginsenoside Re peak), 1 (the ginsenoside Rg1 peak is namely with reference to the peak), 2.58-2.67,0.68-2.76,2.77-2.81, (2.82-2.91 ginsenoside Rb1 peak), 2.95-3.03,3.05-3.13, (3.15-3.22 ginsenoside Rd peak), 3.24-3.91.In the total peak unimodal area account for total peak area greater than 20% ginsenoside Rg1 peak and ginsenoside Rb1 peak arranged.Ginsenoside Rg1's peak area (namely with reference to the peak) accounts for the 20%-35% of total peak area, and its relative peak area is 1; Ginsenoside Rb1's peak area accounts for the 30%-50% of total peak area, and its relative peak area is 0.85-2.50; The arasaponin R1 peak area accounts for the 2%-8% of total peak area, and its relative peak area is 0.06-0.40; Ginsenoside Rd's peak area accounts for the 5%-14% of total peak area, and its relative peak area is 0.14-0.70.The non-total peak gross area is not more than 10% of total peak area.
Borneolum Syntheticum in the above-mentioned Chinese medicine composition is artificial Borneolum Syntheticum or natural Broneolum Syntheticum.
Lignum Dalbergiae Odoriferae oil in the above-mentioned Chinese medicine composition is that Lignum Dalbergiae Odoriferae is through the distillation gained.
Pharmaceutically acceptable carrier of the present invention can be any preparation Chinese medicinal granule pharmaceutically acceptable carrier commonly used or conventional, and for example: diluent (filler) includes but not limited to sucrose, dextrin, starch, lactose, mannitol, xylitol, chitosan, SHUANGQITANG, soluble starch, Pulvis Talci or water solublity dextrin etc.; Disintegrating agent includes but not limited to starch, sodium carboxymethyl cellulose (CMS-Na), microcrystalline Cellulose (MCC), micropowder silica gel, hydroxypropyl starch, soluble starch, water solublity dextrin; Inclusion agents includes but not limited to alpha-cyclodextrin (α-CD), beta-schardinger dextrin-(β-CD) and N-LOK modified starch etc.; Wetting agent (binding agent) includes but not limited to water, ethanol, polyvinylpyrrolidone (polyvidone), hydroxypropyl cellulose, Polyethylene Glycol above-mentioned adjuvant function such as diluent such as (PEG), disintegrating agent, wetting agent is called by function in this patent, be viscosity modifier, preferably microcrystalline cellulose (MCC), micropowder silica gel, Polyethylene Glycol, Pulvis Talci, chitosan, Pulvis Talci, the above-mentioned pharmaceutically acceptable carrier of polyvidone can use separately, also can unite use.Persons of ordinary skill in the art may appreciate that the following emerging pharmaceutically acceptable carrier that can be used for preparing Chinese medicinal granule, if can realize purpose of the present invention, also should be included in protection scope of the present invention.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, and described pharmaceutically acceptable carrier preferably one or more in sucrose, dextrin, starch, lactose, mannitol, xylitol, chitosan, SHUANGQITANG, soluble starch, Pulvis Talci, water solublity dextrin sodium carboxymethyl cellulose (CMS-Na), microcrystalline Cellulose (MCC), micropowder silica gel, hydroxypropyl starch, ethanol, hydroxypropyl cellulose, Polyethylene Glycol, chitosan, polyvidone is united use.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, and described pharmaceutically acceptable carrier is preferably: crystalline cellulose element, micropowder silica gel, Polyethylene Glycol, Pulvis Talci, chitosan, Pulvis Talci, polyvidone.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, described granule comprises master batch and the shell that is positioned on the master batch, and profile is sphere or class sphere, and bulk density is 0.6~1.3g/ml, dissolve scattered time limit is 0.4~5 minute, and active constituents of medicine is included among master batch and/or the shell.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, and its particle diameter is 700~1500 μ m.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, and described granule also contains coating, and the weight of coating materials accounts for 2~5wt% of granule gross weight.
The Chinese medicinal granule that the present invention contains Radix Notoginseng extract adopts the fluidized bed granulation technology to granulate, common fluidized bed granulation technology is directly packed into powdered substance and is granulated as bed material in the container of fluid bed, and the present invention forms with pharmaceutically acceptable preparing carriers, perhaps is prepared from by pharmaceutically acceptable carrier and corresponding Chinese medicine extract dry powder.
The present invention contains the preparation method of the Chinese medicinal granule of Radix Notoginseng extract, and step is as follows:
(1) preparation of active component of the present invention: Radix Salviae Miltiorrhizae extract 5.0%~80.0%, Radix Notoginseng extract 15.0%~93.0% and Borneolum Syntheticum or Lignum Dalbergiae Odoriferae oil 2.0%~15.0%, mix homogeneously, preparation cost invention active component;
(2) preparation of master batch: i. gets an amount of pharmaceutically acceptable carrier, the perhaps mixture of itself and Chinese medicine extract dry powder, and crushing screening obtains the satisfactory material of granularity; Ii. get the material of a part of step I, drop in the spray of fluid bed side or the end spray pot, other gets it filled and learns acceptable carrier and/or Chinese medicine extract and add water and be mixed with slurry, adopt the spray of fluid bed side or end pressure spray process to spray into, and sift out granule as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, the material of remaining step I is sprinkled into fine powder in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, filter out the satisfactory master batch of particle diameter;
(3) preparation of product: it is an amount of to get master batch, and acceptable carrier is an amount of, and active component of the present invention is an amount of; Above-mentioned acceptable carrier is added in the invention described above active component, and it is standby as slurry to mix into suspension; Above-mentioned master batch is dropped in the fluid bed side spray pot, and above-mentioned slurry slowly sprays into, and all sprays into to slurry, makes spheroidal particle.
The present invention contains the preparation method of the Chinese medicinal granule of Radix Notoginseng extract, and preferred steps is as follows:
(1) preparation of active component of the present invention: get Radix Salviae Miltiorrhizae extract 30.3%, Radix Notoginseng extract 60.6% and Borneolum Syntheticum or Lignum Dalbergiae Odoriferae oil 9.1%, mix homogeneously, preparation cost invention active component;
(2) preparation of master batch: the mixture of getting dextrin and starch weight ratio and being 1: 1 is crossed 200 mesh sieves, and wherein 60-65wt% drops in the fluid bed side spray pot as bed material; 10-15wt% dextrin and starch mixture, amount to the active component of the present invention that accounts for the 15wt% of master batch weight after the dry weight, adding water is mixed with slurry and sprays into, and to sift out particle diameter be that 180~250 μ m granules are as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle dextrin of surplus and the fine powder of starch mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, the master batch that filters out particle diameter and be 450~600 μ m is standby;
(3) preparation of product: get master batch 450g, the active component of the present invention of 400g, with active component weight ratio of the present invention be 18: 25 polyethylene glycol 6000, perhaps be 7: 25 HPMC and PVP-K30 mixture, wherein HPMC with active component weight ratio of the present invention: the PVP-K30 weight ratio is 2: 5; With dehydrated alcohol above-mentioned viscosity is adjusted and a kind ofly in agent polyethylene glycol 6000 or HPMC and the PVP-K30 mixture to be formulated as the solution that concentration is 15wt%, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 19wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
The present invention contains in the preparation method of Chinese medicinal granule of Radix Notoginseng extract, and the temperature of charge control of the spheroidal particle that said method can be obtained is at 37~45 ℃; Transparent coating material water is made into the coating solution of 7.5% (g/ml) again, according to the amount of theory weightening finish 3wt% coating solution is sprayed into coating, make spheroidal particle, its bulk density is 0.76g/ml, and dissolve scattered time limit is 25~180 seconds.
The present invention contains in the preparation method of Chinese medicinal granule of Radix Notoginseng extract, No. 2 capsules of spheroidal particle fill that said method can be obtained, and the 250mg/ grain is made 10000 of capsules.
" setting of dosage form in the Chinese pharmacopoeia, and in order to distinguish different with technology such as micropill, microcapsules in conjunction with the characteristic of present technique preparing product, was named above-mentioned granule and is spheroidized particle according to version in 2005.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, outside it coating can also be arranged, and the weight of coating materials accounts for the 2-5% of granule gross weight.During the spheroidized particle coating, obviously low (plain particles coating, coating materials consumption are 20-30% to the more common granule coating of the consumption of coating materials, and spheroidized particle coating materials consumption then can be reduced to 2-5% with the adjuvant amount.As required, coating can be common film coating, also can be enteric film coating, slow controlled release coat etc., and coating materials can be the commonly used or conventional coating materials in any this area, select according to different needs, the coating process can be carried out according to the method for this area routine.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, directly uses except can be used as common granule, can also be as intermediate, be prepared into dosage forms such as capsule, as granule use etc., can be prepared into sustained-release preparation in addition, positioning release medicine preparation etc.
The present invention contains the Chinese medicinal granule of Radix Notoginseng extract, and preferred described granule is made conventional capsule agent or sustained-release preparation.
Spheroidized particle of the present invention has the following advantages:
1. the consumption of adjuvant is few, therefore causes single dose little, and the general each dose of patient is that 0.1~4g gets final product, and can reduce the fear that the patient takes medicine and produces heavy dose.
2. outward appearance is good, and granule of the present invention is spherical in shape or class is spherical, the smooth surface rounding, the control of suitable product design quality.
3. physical characteristic is good, and in the spheroidal particle preparation process, mobility of particle is good, causes that particle size distribution is regular, the fine and close anti-extruding of quality, and wear-resistant, density is big, and (bulk density is 0.6~1.3g/ml), specific surface area is little (has only 0.01~0.03m
2/ g).
4. dissolve scattered time limit is short, and the spheroidal particle dissolve scattered time limit of the present invention that adopts this type of prescription to make is short, is generally 0.4~5 minute.
5. granularity test: according to 2000 editions pharmacopeia regulations, get granule 5 bags of (bottle) or multiple dose packing granule 1 bags (bottle) of single dose packing, claim to decide weight, put in the medicine sieve and sieve.When sieving, will sieve and keep level, about come and go and sieved gently 3 minutes.Can not and can must not cross 8.0% by granule and the powder summation of No. four sieves by a sieve.Granule of the present invention can not and can meet the pharmacopeia regulation by granule and the powder summation of No. four sieves by a sieve, and its numerical value is no more than 5.5%.
6. melting test: according to 2000 editions pharmacopeia regulations, get medicinal granule test sample 10g of the present invention, add 20 times of hot water, stirred 5 minutes, observe immediately.Soluble granule should all dissolve.
These characteristics can improve patient's the row of complying with, and makes the application of packaging technique become possibility, thereby has solved the moisture absorption (critical wettability is promoted to 85% by 60% of plain particles) of Chinese medicine, problem such as stable; In addition, spheroidized particle of the present invention uses except can be used as common granule, can also be as intermediate or granule, and fill becomes dosage forms such as capsule, can be prepared into sustained-release preparation in addition, positioning release medicine preparation etc.
The specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment, and following this embodiment only for explanation the present invention to the present invention without limits.
Embodiment 1
The preparation of active component of the present invention:
Radix Salviae Miltiorrhizae extract: the preparation method by Chinese patent application (application number 02117923.9) obtains.Concrete extracting method is: Radix Salviae Miltiorrhizae 5kg is ground into coarse powder, adds deionized water heating extraction 3 times under 100 ℃ of slight boiling conditions.5.5 times of water heated 1 hour for the first time; Second and third time respectively adds 3 times of water gagings and heated respectively 0.5 hour.Extracting solution filters after transferring pH value to be 2 with 10% hydrochloric acid, polyamide column on the filtrate (dried resin amount make a living dose 2/3).With the washing of 5 times of amount deionizations, continue with 0.1% sodium bicarbonate aqueous solution eluting, consumption is 5 times of column volume.Collect eluent, transfer PH to 2 back to go up D with 10% hydrochloric acid
101Macroporous adsorbent resin, continues to use 95% ethanol elution to neutral with deionized water rinsing, treats that colour band gets off namely to collect this colour band.Concentrating under reduced pressure is collected liquid to doing to the greatest extent, spend the night with suitable quantity of water dissolving back refrigerator cold-storage, namely get the Radix Salviae Miltiorrhizae total phenolic acids extracting solution by 0.3 μ m cellulose mixture filtering with microporous membrane, with lyophilization immediately behind the 2% sodium hydroxide adjusting pH value to 6.0, Radix Salviae Miltiorrhizae extract raw material lyophilized powder 221g, product yield 4.4% of the dose of making a living;
Buy Radix Notoginseng total arasaponins from market, through further refining, get Radix Notoginseng extract.The ginsenoside Re is 3.9% in the Radix Notoginseng extract, and the ginsenoside Rg1 is 34.3%, and the ginsenoside Rb1 is 31.0%, and the ginsenoside Rd is 8.8%, and arasaponin R1 is 6.8%, and its content of the total saponins in radix notoginseng is 94%;
The Lignum Dalbergiae Odoriferae oil extract is buied in market;
Get above-mentioned above-mentioned Radix Salviae Miltiorrhizae extract 1000g, above-mentioned Radix Notoginseng extract 2000g, Lignum Dalbergiae Odoriferae oil 300g, mix homogeneously, mix homogeneously, the preparation cost invention active component got;
The preparation of master batch: the weight ratio of getting dextrin and starch is that 1: 1 mixture is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets starch and adds water and be mixed with starch slurry (15%) and spray into as slurry, and sift out granule (particle diameter is 180-250 μ) as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% dextrin of remainder and the fine powder of starch are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 450 μ-600 μ to filter out particle diameter.
The preparation of product: get master batch 450g, microcrystalline Cellulose (200 order) 50g, polyethylene glycol 6000 (heating and melting)
500G, active component 10000g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and adding water, to mix into solid content be 30% binding agent; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly being dried to simultaneously slurry all sprays into and makes spheroidal particle, temperature of charge control is at 55 degrees centigrade, be made into 7.5% coating solution again with transparent coating material OPAGLOS 2 waters, amount according to theory weightening finish 3% sprays into coating with coating solution, makes spheroidal particle.Bulk density is 0.76g/ml.With No. 2 capsules of spheroidal particle fill that obtain, every dress 0.125g makes 10000 of capsules.
Embodiment 2
The preparation of active component of the present invention: get the Radix Notoginseng extract 2000g of Radix Salviae Miltiorrhizae extract 1000g, the embodiment one of embodiment one, the Lignum Dalbergiae Odoriferae oil 100g of embodiment one, mix homogeneously, preparation cost invention active component;
The preparation of master batch: the mixture of getting micropowder silica gel and chitosan weight ratio and being 1: 1 is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water, extract, is mixed with bonding agent-spray into as slurry, and to sift out particle diameter be that 120-180 μ granule is as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% micropowder silica gel of remainder and the fine powder of chitosan are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 200 μ-300 μ to filter out particle diameter.
The preparation of product: get master batch 300g, microcrystalline Cellulose (200 order) 250g, micropowder silica gel 14g, active component 2000g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and it is standby as slurry to mix into uniform suspension; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to simultaneously slurry and all spray into and make spheroidal particle, be made into 7.5% coating solution again with transparent coating material OPAGLOS 2 waters, amount according to theory weightening finish 3% sprays into coating with coating solution, makes spheroidal particle.The bulk density 0.93g/ml of granule, with No. 00 capsule of spheroidal particle fill that obtains, every dress 0.70g makes 10000 of capsules.
Embodiment 3
The preparation of active component of the present invention: get the Radix Notoginseng extract 267g of Radix Salviae Miltiorrhizae extract 3300g, the embodiment one of embodiment one, the Lignum Dalbergiae Odoriferae oil 300g of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: the mixture of getting dextrin and starch weight ratio and being 1: 1 is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets starch, extract (account for after giving money as a gift master batch weight 15%) and adds water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ) as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% dextrin of remainder and the fine powder of starch are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 300 μ~400 μ to filter out particle diameter.
The preparation of product: get master batch 450g, microcrystalline Cellulose (200 order) 50g, polyethylene glycol 6000 (heating and melting)
500G, active component 1000g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and adding water, to mix into solid content be 30% binding agent; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly being dried to simultaneously slurry all sprays into and makes spheroidal particle, temperature of charge control is at 55 degrees centigrade, be made into 7.5% coating solution again with transparent coating material OPAGLOS2 water, amount according to theory weightening finish 3% sprays into coating with coating solution, makes spheroidal particle, and bulk density is 0.76g/ml; With No. 2 capsules of spheroidal particle fill that obtain, every dress 0.125g makes 10000 of capsules.
Embodiment 4
The preparation of active component of the present invention: get the Radix Notoginseng extract 2400g of Radix Salviae Miltiorrhizae extract 600g, the embodiment one of embodiment one, the Lignum Dalbergiae Odoriferae oil 300g of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: the mixture of getting dextrin and microcrystalline Cellulose weight ratio and being 1: 1 is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water and be mixed with bonding agent (5%) and spray into as slurry, and to sift out particle diameter be that 180~250 μ granules are as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% dextrin of remainder and the fine powder of microcrystalline Cellulose are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 300 μ~400 μ to filter out particle diameter.
The preparation of product: get master batch 1000g, in 5% the polyvidone solution, add active component of the present invention an amount of (being equivalent to 20 kilograms of medical materials); In 5% the polyvidone solution, add that active component of the present invention is an amount of, adding water, to mix into solid content be that 30% suspension is standby as slurry; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to simultaneously slurry and all spray into and make spheroidal particle, be made into 7.5% coating solution again with transparent coating material OPAGLOS 2 waters, amount according to theory weightening finish 3% sprays into coating with coating solution, makes spheroidal particle.
Embodiment 5
The preparation of active component of the present invention: get the Radix Notoginseng extract 1500g of Radix Salviae Miltiorrhizae extract 1500g, the embodiment one of embodiment one, the Lignum Dalbergiae Odoriferae oil 300g of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: the mixture of getting dextrin and microcrystalline Cellulose weight ratio and being 2: 1 is crossed 100 mesh sieves, wherein 75% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water, extract, is mixed with bonding agent-spray into as slurry, and to sift out particle diameter be that 100-200 μ granule is as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% dextrin of remainder and the fine powder of microcrystalline Cellulose are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 200 μ-300 μ to filter out particle diameter.
The preparation of product: get master batch 800g, microcrystalline Cellulose 190g, micropowder silica gel 35g, active component 2500g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and it is standby as slurry to mix into uniform suspension; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 6
The composition of active component of the present invention: get Radix Notoginseng extract 1000g, the Lignum Dalbergiae Odoriferae oil 300g of Radix Salviae Miltiorrhizae extract 2000g, the embodiment one of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: the mixture of getting micropowder silica gel and Pulvis Talci weight ratio and being 1: 1 is crossed 200 mesh sieves, wherein 50% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water, extract, is mixed with bonding agent-spray into as slurry, and to sift out particle diameter be that 120-180 μ granule is as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% micropowder silica gel and talcous fine powder of remainder is sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 200 μ-300 μ to filter out particle diameter.
The preparation of product: get master batch 300g, microcrystalline Cellulose (200 order) 250g, micropowder silica gel 14g, active component 2000g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and it is standby as slurry to mix into uniform suspension; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 7
The preparation of active component of the present invention: get the Radix Notoginseng extract 2930g of Radix Salviae Miltiorrhizae extract 170g, the embodiment one of embodiment one, the Lignum Dalbergiae Odoriferae oil 200g of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: taking polyethylene glycol and chitosan weight ratio are that 1: 1 mixture is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water, extract, is mixed with bonding agent-spray into as slurry, and to sift out particle diameter be that 120-180 μ granule is as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% Polyethylene Glycol of remainder and the fine powder of chitosan are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 200 μ-300 μ to filter out particle diameter.
The preparation of product: get master batch 700g, microcrystalline Cellulose (200 order) 300g, micropowder silica gel 20g, active component 1500g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and it is standby as slurry to mix into uniform suspension; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 8
1, the preparation of active component of the present invention
Get the Radix Notoginseng extract 1650g of Radix Salviae Miltiorrhizae extract 1170g, the embodiment one of embodiment one, the Lignum Dalbergiae Odoriferae oil 480g of embodiment one, mix homogeneously is made active component of the present invention.
2, the preparation of master batch
Get dextrin and starch (1: 1) mixture and cross 200 mesh sieves, wherein 65wt% drops in the fluid bed side spray pot, other gets starch and adds water and be mixed with starch slurry (15%, g/ml) spray into as slurry, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state starch slurry, remaining 35% dextrin and the fine powder of starch (1: 1) mixture are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 450~600 μ m to filter out particle diameter.
3, viscosity is adjusted the selection of agent
A. get the invention described above active component, be diluted to the diluent that viscosity is 6.0~9.8MpaS with the alcoholic solution of 60% (ml/ml).
B. get 1 above-mentioned diluent, drop on the microscope slide, hang, solid cicatrix surface, liquid evaporation back does not have complete film, and viscosity is less, easily scrapes with powdery, and it is serious to play powder.
C. the viscosity according to cicatrix among the b is little, film property is poor, intensity is little, easily play characteristics such as powder, select viscosity to adjust agent, experiment shows can select HPMC: PVP-K30 (2: 5) mixture to adjust agent as viscosity, and also can select polyethylene glycol 6000 is that viscosity is adjusted agent.
D. selection and active component weight ratio of the present invention are 7: 25 HPMC: PVP-K30 (2: 5) mixture is that viscosity is adjusted agent, perhaps selection and active component weight ratio of the present invention are that 18: 25 polyethylene glycol 6000 is that viscosity is adjusted agent, and material viscosity is increased to 16MPaS by 9.0MPaS.
4, the preparation of spheroidal particle of the present invention
A. get master batch 450g, with active component weight ratio of the present invention be 18: 25 polyethylene glycol 6000, perhaps be 7: 25 HPMC with active component weight ratio of the present invention: PVP-K30 (2: 5) mixture, the active component of the present invention of 10000g;
B. with dehydrated alcohol above-mentioned viscosity is adjusted and a kind ofly in the agent be formulated as the solution that concentration is 15wt%, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 19wt%, as slurry;
C. master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly being dried to simultaneously slurry all sprays into and makes spheroidal particle, temperature of charge control is at 37~45 ℃, again transparent coating material water is made into the coating solution of 7.5% (g/ml), according to the amount of theory weightening finish 3wt% coating solution is sprayed into coating, make spheroidal particle, its bulk density is 0.76g/ml, and dissolve scattered time limit is 25 seconds.
With No. 2 capsules of spheroidal particle fill that obtain among the step c, the 250g/ grain is made 10000 of capsules.
Embodiment 9
1, the preparation of active component of the present invention: get Radix Salviae Miltiorrhizae extract 1360g (Chinese patent CN1352985A embodiment 1), the Radix Notoginseng extract 1800g of embodiment one, the Lignum Dalbergiae Odoriferae oil 140g of embodiment one, mix homogeneously, preparation cost invention active component.
2, the preparation of master batch: get xylitol and carboxymethyl starch sodium (1: 1) mixture and cross 200 mesh sieves, wherein 60wt% drops in the fluid bed side spray pot, other gets 10wt% xylitol and carboxymethyl starch sodium (1: 1) mixture and active component of the present invention (after amounting to dry weight, account for the 15wt% of master batch weight) add water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle, the xylitol of remaining 30wt% and the fine powder of carboxymethyl starch sodium (1: 1) mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, and the master batch that filters out particle diameter and be 280~450 μ m is standby.
3, the preparation of product: get master batch 450g, the active component of the present invention of 3000g, with active component weight ratio of the present invention be 18: 25 polyethylene glycol 6000, with dehydrated alcohol above-mentioned viscosity is adjusted the agent polyethylene glycol 6000 and be formulated as the solution that concentration is 15wt%, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 19wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 10
1, the preparation of active component of the present invention: get Radix Salviae Miltiorrhizae extract 1200g (Chinese patent CN1384090A embodiment 1), Radix Notoginseng extract 1700g (Tang's light, Chinese patent medicine, 1990,5), the Lignum Dalbergiae Odoriferae oil 380g of embodiment one 12 (8):, with the above-mentioned substance mix homogeneously, make active component of the present invention.
2, the preparation of master batch: get lactose and hydroxypropyl starch (1: 1) mixture and cross 200 mesh sieves, wherein 60wt% drops in the fluid bed side spray pot, other gets 10wt% lactose and hydroxypropyl starch (1: 1) mixture and active component of the present invention (after amounting to dry weight, account for the 15wt% of master batch weight) add water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle, the lactose of remaining 30wt% and the fine powder of hydroxypropyl starch (1: 1) mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, and the master batch that filters out particle diameter and be 180~250 μ m is standby.
3, the preparation of product: get master batch 850g, the active component of the present invention of 200g, with active component weight ratio of the present invention be 7: 25 HPMC and PVP-K30 mixture, wherein HPMC: the PVP-K30 weight ratio is 2: 5; Above-mentioned viscosity is adjusted agent HPMC and the PVP-K30 mixture is formulated as the solution that concentration is 15wt% with dehydrated alcohol, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 20wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 11
1, the preparation of active component of the present invention: get Radix Salviae Miltiorrhizae extract 2630g (Chinese patent CN1384090A embodiment 1), the Radix Notoginseng extract 500g of embodiment one, the Lignum Dalbergiae Odoriferae oil 170g of embodiment one, with the above-mentioned substance mix homogeneously, make active component of the present invention.
2, the preparation of master batch: get dextrin and mannitol (3: 1) mixture and cross 200 mesh sieves, wherein 64wt% drops in the fluid bed side spray pot, other gets 16wt% dextrin and mannitol mixture and active component of the present invention (after amounting to dry weight, account for the 10wt% of master batch weight) add water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle, the dextrin of remaining 20wt% and the fine powder of mannitol mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, and the master batch that filters out particle diameter and be 450~600 μ m is standby.
3, the preparation of product: get master batch 500g, the active component of the present invention of 1500g, with active component weight ratio of the present invention be 7: 25 HPMC and PVP-K30 mixture, wherein HPMC: the PVP-K30 weight ratio is 2: 5; Above-mentioned viscosity is adjusted agent HPMC and the PVP-K30 mixture is formulated as the solution that concentration is 15wt% with dehydrated alcohol, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 20wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 12
1, the preparation of active component of the present invention: get Radix Salviae Miltiorrhizae extract 180g (Chinese patent CN1384090A embodiment 1), embodiment one Radix Notoginseng extract 3050g, Borneolum Syntheticum 70g, with the above-mentioned substance mix homogeneously, make active component of the present invention.
2, the preparation of master batch: get dextrin and mannitol (2: 1) mixture and cross 200 mesh sieves, wherein 65wt% drops in the fluid bed side spray pot, other gets 25wt% dextrin and mannitol mixture and active component of the present invention (after amounting to dry weight, account for the 10wt% of master batch weight) add water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle, the dextrin of remaining 10wt% and the fine powder of mannitol mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, and the master batch that filters out particle diameter and be 450~600 μ m is standby.
3, the preparation of product: get master batch 450g, the active component of the present invention of 4000g, with active component weight ratio of the present invention be 18: 25 polyethylene glycol 6000, with dehydrated alcohol above-mentioned viscosity is adjusted the agent polyethylene glycol 6000 and be formulated as the solution that concentration is 15wt%, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 19wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 13
The preparation of active component of the present invention:
Radix Salviae Miltiorrhizae extract: the preparation method by Chinese patent application (application number 02117923.9) obtains.Concrete extracting method is: Radix Salviae Miltiorrhizae 5kg is ground into coarse powder, adds deionized water heating extraction 3 times under 100 ℃ of slight boiling conditions.5.5 times of water heated 1 hour for the first time; Second and third time respectively adds 3 times of water gagings and heated respectively 0.5 hour.Extracting solution filters after transferring pH value to be 2 with 10% hydrochloric acid, polyamide column on the filtrate (dried resin amount make a living dose 2/3).With the washing of 5 times of amount deionizations, continue with 0.1% sodium bicarbonate aqueous solution eluting, consumption is 5 times of column volume.Collect eluent, transfer PH to 2 back to go up D with 10% hydrochloric acid
101Macroporous adsorbent resin, continues to use 95% ethanol elution to neutral with deionized water rinsing, treats that colour band gets off namely to collect this colour band.Concentrating under reduced pressure is collected liquid to doing to the greatest extent, spend the night with suitable quantity of water dissolving back refrigerator cold-storage, namely get the Radix Salviae Miltiorrhizae total phenolic acids extracting solution by 0.3 μ m cellulose mixture filtering with microporous membrane, with lyophilization immediately behind the 2% sodium hydroxide adjusting pH value to 6.0, Radix Salviae Miltiorrhizae extract raw material lyophilized powder 221g, product yield 4.4% of the dose of making a living;
Buy Radix Notoginseng total arasaponins from market, through further refining, get Radix Notoginseng extract.The ginsenoside Re is 3.9% in the Radix Notoginseng extract, and the ginsenoside Rg1 is 34.3%, and the ginsenoside Rb1 is 31.0%, and the ginsenoside Rd is 8.8%, and arasaponin R1 is 6.8%, and its content of the total saponins in radix notoginseng is 94%;
Buy Radix Astragali extract from market, through further refining, get Radix Astragali extract.Astragaloside content is 9.5% in the Radix Astragali extract, and the content of Radix Astragali extract is 88.9%;
Get above-mentioned above-mentioned Radix Salviae Miltiorrhizae extract 1000g, above-mentioned Radix Notoginseng extract 2000g, Borneolum Syntheticum 300g, mix homogeneously, mix homogeneously, the preparation cost invention active component got;
The preparation of master batch: the weight ratio of getting dextrin and starch is that 1: 1 mixture is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets starch and adds water and be mixed with starch slurry (15%) and spray into as slurry, and sift out granule (particle diameter is 180-250 μ) as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% dextrin of remainder and the fine powder of starch are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 450 μ-600 μ to filter out particle diameter.
The preparation of product: get master batch 450g, microcrystalline Cellulose (200 order) 50g, polyethylene glycol 6000 (heating and melting)
500G, active component 100g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and adding water, to mix into solid content be 30% binding agent; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly being dried to simultaneously slurry all sprays into and makes spheroidal particle, temperature of charge control is at 55 degrees centigrade, be made into 7.5% coating solution again with transparent coating material OPAGLOS 2 waters, amount according to theory weightening finish 3% sprays into coating with coating solution, makes spheroidal particle.Bulk density is 0.76g/ml.With No. 2 capsules of spheroidal particle fill that obtain, every dress 0.125g makes 10000 of capsules.
Embodiment 14
The preparation of active component of the present invention: get Radix Notoginseng extract 2005g, the Lignum Dalbergiae Odoriferae oil 205g of Radix Salviae Miltiorrhizae extract 1005g, the embodiment one of embodiment one, mix homogeneously, preparation cost invention active component;
The preparation of master batch: the mixture of getting micropowder silica gel and chitosan weight ratio and being 1: 1 is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water, extract, is mixed with bonding agent-spray into as slurry, and to sift out particle diameter be that 120-180 μ granule is as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% micropowder silica gel of remainder and the fine powder of chitosan are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 200 μ-300 μ to filter out particle diameter.
The preparation of product: get master batch 300g, microcrystalline Cellulose (200 order) 250g, micropowder silica gel 14g, active component 2000g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and it is standby as slurry to mix into uniform suspension; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to simultaneously slurry and all spray into and make spheroidal particle, be made into 7.5% coating solution again with transparent coating material OPAGLOS 2 waters, amount according to theory weightening finish 3% sprays into coating with coating solution, makes spheroidal particle.The bulk density 0.93g/ml of granule, with No. 00 capsule of spheroidal particle fill that obtains, every dress 0.70g makes 10000 of capsules.
Embodiment 15
The preparation of active component of the present invention: get Radix Notoginseng extract 2670g, the Borneolum Syntheticum 290g of Radix Salviae Miltiorrhizae extract 330g, the embodiment one of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: the mixture of getting dextrin and starch weight ratio and being 1: 1 is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets starch, extract (account for after giving money as a gift master batch weight 15%) and adds water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ) as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% dextrin of remainder and the fine powder of starch are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 300 μ~400 μ to filter out particle diameter.
The preparation of product: get master batch 450g, microcrystalline Cellulose (200 order) 50g, polyethylene glycol 6000 (heating and melting)
500G, active component 1000g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and adding water, to mix into solid content be 30% binding agent; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly being dried to simultaneously slurry all sprays into and makes spheroidal particle, temperature of charge control is at 55 degrees centigrade, be made into 7.5% coating solution again with transparent coating material OPAGLOS2 water, amount according to theory weightening finish 3% sprays into coating with coating solution, makes spheroidal particle, and bulk density is 0.76g/ml; With No. 2 capsules of spheroidal particle fill that obtain, every dress 0.125g makes 10000 of capsules.
Embodiment 16
The preparation of active component of the present invention: get Radix Notoginseng extract 2400g, the Borneolum Syntheticum 300g of Radix Salviae Miltiorrhizae extract 600g, the embodiment one of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: the mixture of getting dextrin and microcrystalline Cellulose weight ratio and being 1: 1 is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water and be mixed with bonding agent (5%) and spray into as slurry, and to sift out particle diameter be that 180~250 μ granules are as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% dextrin of remainder and the fine powder of microcrystalline Cellulose are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 300 μ~400 μ to filter out particle diameter.
The preparation of product: get master batch 1000g, in 5% the polyvidone solution, add active component of the present invention an amount of (being equivalent to 20 kilograms of medical materials); In 5% the polyvidone solution, add that active component of the present invention is an amount of, adding water, to mix into solid content be that 30% suspension is standby as slurry; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to simultaneously slurry and all spray into and make spheroidal particle, be made into 7.5% coating solution again with transparent coating material OPAGLOS 2 waters, amount according to theory weightening finish 3% sprays into coating with coating solution, makes spheroidal particle.
Embodiment 17
The preparation of active component of the present invention: get Radix Notoginseng extract 1500g, the Borneolum Syntheticum 30g of Radix Salviae Miltiorrhizae extract 1500g, the embodiment one of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: the mixture of getting dextrin and microcrystalline Cellulose weight ratio and being 2: 1 is crossed 100 mesh sieves, wherein 75% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water, extract, is mixed with bonding agent-spray into as slurry, and to sift out particle diameter be that 100-200 μ granule is as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% dextrin of remainder and the fine powder of microcrystalline Cellulose are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 200 μ-300 μ to filter out particle diameter.
The preparation of product: get master batch 800g, microcrystalline Cellulose 190g, micropowder silica gel 35g, active component 2500g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and it is standby as slurry to mix into uniform suspension; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 18
The composition of active component of the present invention: get Radix Notoginseng extract 100g, the Borneolum Syntheticum 30g of Radix Salviae Miltiorrhizae extract 200g, the embodiment one of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: the mixture of getting micropowder silica gel and Pulvis Talci weight ratio and being 1: 1 is crossed 200 mesh sieves, wherein 50% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water, extract, is mixed with bonding agent-spray into as slurry, and to sift out particle diameter be that 120-180 μ granule is as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% micropowder silica gel and talcous fine powder of remainder is sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 200 μ-300 μ to filter out particle diameter.
The preparation of product: get master batch 300g, microcrystalline Cellulose (200 order) 250g, micropowder silica gel 14g, active component 2000g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and it is standby as slurry to mix into uniform suspension; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 19
The preparation of active component of the present invention: get Radix Notoginseng extract 2930g, the Borneolum Syntheticum 200g of Radix Salviae Miltiorrhizae extract 170g, the embodiment one of embodiment one, mix homogeneously, preparation cost invention active component.
The preparation of master batch: taking polyethylene glycol and chitosan weight ratio are that 1: 1 mixture is crossed 200 mesh sieves, wherein 65% as in the bed material input fluid bed side spray pot, other gets PVP K30 and adds water, extract, is mixed with bonding agent-spray into as slurry, and to sift out particle diameter be that 120-180 μ granule is as parent nucleus, again parent nucleus is dropped in the pot, continue the spray starch slurry, 35% Polyethylene Glycol of remainder and the fine powder of chitosan are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 200 μ-300 μ to filter out particle diameter.
The preparation of product: get master batch 700g, microcrystalline Cellulose (200 order) 300g, micropowder silica gel 20g, active component 1500g of the present invention; Microcrystalline Cellulose and micropowder silica gel are added in the active component of the present invention, and it is standby as slurry to mix into uniform suspension; Master batch is dropped in the fluid bed side spray pot, slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 20:
1, the preparation of active component of the present invention
Get Radix Notoginseng extract 1650g, the Borneolum Syntheticum 480g of Radix Salviae Miltiorrhizae extract 1170g, the embodiment one of embodiment one, mix homogeneously is made active component of the present invention.
2, the preparation of master batch
Get dextrin and starch (1: 1) mixture and cross 200 mesh sieves, wherein 65wt% drops in the fluid bed side spray pot, other gets starch and adds water and be mixed with starch slurry (15%, g/ml) spray into as slurry, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state starch slurry, remaining 35% dextrin and the fine powder of starch (1: 1) mixture are sprinkled in the dusting rifle simultaneously, the particle diameter of parent nucleus is grown up, and it is standby at the master batch of 450~600 μ m to filter out particle diameter.
3, viscosity is adjusted the selection of agent
A. get the invention described above active component, be diluted to the diluent that viscosity is 6.0~9.8MpaS with the alcoholic solution of 60% (ml/ml).
B. get 1 above-mentioned diluent, drop on the microscope slide, hang, solid cicatrix surface, liquid evaporation back does not have complete film, and viscosity is less, easily scrapes with powdery, and it is serious to play powder.
C. the viscosity according to cicatrix among the b is little, film property is poor, intensity is little, easily play characteristics such as powder, select viscosity to adjust agent, experiment shows can select HPMC: PVP-K30 (2: 5) mixture to adjust agent as viscosity, and also can select polyethylene glycol 6000 is that viscosity is adjusted agent.
D. selection and active component weight ratio of the present invention are 7: 25 HPMC: PVP-K30 (2: 5) mixture is that viscosity is adjusted agent, perhaps selection and active component weight ratio of the present invention are that 18: 25 polyethylene glycol 6000 is that viscosity is adjusted agent, and material viscosity is increased to 16MPaS by 9.0MPaS.
4, the preparation of spheroidal particle of the present invention
D. get master batch 450g, with active component weight ratio of the present invention be 18: 25 polyethylene glycol 6000, perhaps be 7: 25 HPMC with active component weight ratio of the present invention: PVP-K30 (2: 5) mixture, the active component of the present invention of 10000g;
E. with dehydrated alcohol above-mentioned viscosity is adjusted and a kind ofly in the agent be formulated as the solution that concentration is 15wt%, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 19wt%, as slurry;
F. master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly being dried to simultaneously slurry all sprays into and makes spheroidal particle, temperature of charge control is at 37~45 ℃, again transparent coating material water is made into the coating solution of 7.5% (g/ml), according to the amount of theory weightening finish 3wt% coating solution is sprayed into coating, make spheroidal particle, its bulk density is 0.76g/ml, and dissolve scattered time limit is 25 seconds.
With No. 2 capsules of spheroidal particle fill that obtain among the step c, the 250g/ grain is made 10000 of capsules.
Embodiment 21
1, the preparation of active component of the present invention: get Radix Notoginseng extract 1800g, the Borneolum Syntheticum 130g of Radix Salviae Miltiorrhizae extract 1360g (Chinese patent CN1352985A embodiment 1), embodiment one, mix homogeneously, preparation cost invention active component.
2, the preparation of master batch: get xylitol and carboxymethyl starch sodium (1: 1) mixture and cross 200 mesh sieves, wherein 60wt% drops in the fluid bed side spray pot, other gets 10wt% xylitol and carboxymethyl starch sodium (1: 1) mixture and active component of the present invention (after amounting to dry weight, account for the 15wt% of master batch weight) add water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle, the xylitol of remaining 30wt% and the fine powder of carboxymethyl starch sodium (1: 1) mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, and the master batch that filters out particle diameter and be 280~450 μ m is standby.
3, the preparation of product: get master batch 450g, the active component of the present invention of 3000g, with active component weight ratio of the present invention be 18: 25 polyethylene glycol 6000, with dehydrated alcohol above-mentioned viscosity is adjusted the agent polyethylene glycol 6000 and be formulated as the solution that concentration is 15wt%, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 19wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 22
1, the preparation of active component of the present invention: get Radix Salviae Miltiorrhizae extract 1200g (Chinese patent CN1384090A embodiment 1), Radix Notoginseng extract 1700g (Tang's light, Chinese patent medicine, 5), Borneolum Syntheticum 380g 1990,12 (8):, with the above-mentioned substance mix homogeneously, make active component of the present invention.
2, the preparation of master batch: get lactose and hydroxypropyl starch (1: 1) mixture and cross 200 mesh sieves, wherein 60wt% drops in the fluid bed side spray pot, other gets 10wt% lactose and hydroxypropyl starch (1: 1) mixture and active component of the present invention (after amounting to dry weight, account for the 15wt% of master batch weight) add water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle, the lactose of remaining 30wt% and the fine powder of hydroxypropyl starch (1: 1) mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, and the master batch that filters out particle diameter and be 180~250 μ m is standby.
3, the preparation of product: get master batch 850g, the active component of the present invention of 2000g, with active component weight ratio of the present invention be 7: 25 HPMC and PVP-K30 mixture, wherein HPMC: the PVP-K30 weight ratio is 2: 5; Above-mentioned viscosity is adjusted agent HPMC and the PVP-K30 mixture is formulated as the solution that concentration is 15wt% with dehydrated alcohol, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 20wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 23
1, the preparation of active component of the present invention: get Radix Notoginseng extract 500g, the Borneolum Syntheticum 170g of Radix Salviae Miltiorrhizae extract 2630g (Chinese patent CN1384090A embodiment 1), embodiment one, with the above-mentioned substance mix homogeneously, make active component of the present invention.
2, the preparation of master batch: get dextrin and mannitol (3: 1) mixture and cross 200 mesh sieves, wherein 64wt% drops in the fluid bed side spray pot, other gets 16wt% dextrin and mannitol mixture and active component of the present invention (after amounting to dry weight, account for the 10wt% of master batch weight) add water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle, the dextrin of remaining 20wt% and the fine powder of mannitol mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, and the master batch that filters out particle diameter and be 450~600 μ m is standby.
3, the preparation of product: get master batch 500g, the active component of the present invention of 1200g, with active component weight ratio of the present invention be 7: 25 HPMC and PVP-K30 mixture, wherein HPMC: the PVP-K30 weight ratio is 2: 5; Above-mentioned viscosity is adjusted agent HPMC and the PVP-K30 mixture is formulated as the solution that concentration is 15wt% with dehydrated alcohol, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 20wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.
Embodiment 24
1, the preparation of active component of the present invention: get Radix Salviae Miltiorrhizae extract 180g (Chinese patent CN1384090A embodiment 1), embodiment one Radix Notoginseng extract 3050g, Borneolum Syntheticum 70g, with the above-mentioned substance mix homogeneously, make active component of the present invention.
2, the preparation of master batch: get dextrin and mannitol (2: 1) mixture and cross 200 mesh sieves, wherein 65wt% drops in the fluid bed side spray pot, other gets 25wt% dextrin and mannitol mixture and active component of the present invention (after amounting to dry weight, account for the 10wt% of master batch weight) add water and be mixed with slurry and spray into, and sift out granule (particle diameter is 180~250 μ m) as parent nucleus, again parent nucleus is dropped in the pot, continue to spray and state slurry, in the dusting rifle, the dextrin of remaining 10wt% and the fine powder of mannitol mixture are sprinkled into simultaneously, the particle diameter of parent nucleus is grown up, and the master batch that filters out particle diameter and be 450~600 μ m is standby.
3, the preparation of product: get master batch 450g, the active component of the present invention of 4000g, with active component weight ratio of the present invention be 18: 25 polyethylene glycol 6000, with dehydrated alcohol above-mentioned viscosity is adjusted the agent polyethylene glycol 6000 and be formulated as the solution that concentration is 15wt%, add in the active component of the present invention, after the mixing, adding 60% (ml/ml) ethanol, to transfer to solids content be 19wt%, as slurry; Master batch is dropped in the fluid bed side spray pot, above-mentioned slurry is slowly sprayed into, constantly be dried to slurry simultaneously and all spray into and make spheroidal particle.