CN101438665A - Biological integrated control method for diseases of garden stuff and economic crop - Google Patents
Biological integrated control method for diseases of garden stuff and economic crop Download PDFInfo
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Abstract
The invention relates to a biological integrated disease control method of fruits, vegetables and economic crops, in particular to a complex application method of a biological anti-bacterial agent containing Bacillus subtilis BS2-5, BS6 and BS8-2 bacteria and a bacterial manure containing biochemical fulvic acid. The method comprises the following steps: mixing the diluted biological anti-bacterial agent and a prepared biochemical fulvic acid foliage fertilizer and then spraying the mixture to leaf surfaces of the fruits and the vegetables every 10-20 days after plants start budding according to the growth requirement of branches and leaves of the fruits, the vegetables and the economic crops; and simultaneously flushing or irrigating roots of the fruits and the vegetables with a water flush fertilizer containing the biochemical fulvic acid. The method can cause the biological anti-bacterial agent and the bacterial manure containing biochemical fulvic acid to give full play by the complex application thereof, inhibit and kill pathogenic bacteria on the leaf surfaces and around the roots of the plant by beneficial microbial colonies, play a role of reducing the fertilizer consumption by replacing a chemical pesticide control on the plant diseases with a biological integrated control on the plant diseases, thus realizing green production of the economic crops and achieving a purpose of safe consumption, realizing a process of pollution-free and green production.
Description
Technical field
The biological synthesis that the present invention relates to a kind of fruits and vegetables and economic crops disease is prevented and treated method.Relate in particular to and a kind ofly contain bacillus subtilis (Bacillus subtilis) BS2-5, BS6, the control fruits and vegetables of BS8-3 and the biological antagonist microbial inoculum of economic crops disease, and with a kind of fruits and vegetables of this biological antagonist microbial inoculum and the compound use of fulvic acid bacterial manure and the biological control method of economic crops disease.
Background technology
" fruits and vegetables " are meant the general designation of fruit and vegetables such as apple, pears, kiwi fruit, grape, strawberry, Chinese cabbage, cucumber, romaine lettuce among the present invention; " economic crops " are meant that tea tree, FLOS CHRYSANTHEMI ALBA from Haizhou of China, folium isatidis, honeysuckle, the capsule of weeping forsythia, the bighead atractylodes rhizome etc. can drink plant and can be with the medicinal plant of positions such as its leaf, stem, root as the Chinese medicinal herbs raw material as beverage raw material with its leaf, flower part among the present invention.In fruits and vegetables and economic crops cultivation process, disease generation kind is many and harm is serious, and for example the disease that generally takes place of fruit has: alternaria leaf spot of apple, apple marssonina leaf spot, apple graywall, apple zonate spot, kiwi berry bacterial canker, kiwi fruit leaf rot, kiwi fruit brown spot, pears black heart, pears gray mold, fruit white rot of grape, downy mildew of garpe, grape sour rot, bitter rot or anthracnose of grape; The disease that vegetables are generally taken place has: spot defoliation, early blight of tomato, graw mold of tomato, cladosporium leaf and fruit mould of tomato, head blight, thorn dish spore disease, cucumber downy mildew, cucumber fusarium axysporum, Chinese cabbage virus disease, Chinese cabbage downy mildew and soft rot of cabbage; The disease that tea tree is mainly taken place has: tea shoot rot, tea white star, tea root knot, tea moire blight, tea gall, tea black rot; The various Chinese medicinal herbses of artificial planting common disease etc.At present, the means of controlling plant diseases mainly are to spray chemical bactericide.The normally used chemical bactericide of peasant of plantation fruits and vegetables and economic crops mainly contains: alkene azoles alcohol, polyoxin, iprodione, execute Trane, kasugarnycin, Sandofan, Sukeling, thiophanate methyl, carbendazim, agricultural streptomycin, mancozeb, tpn, Pu Like etc.For preventing and treating disease, the way that adopts normally sprayed chemical pesticide once in average per 3～7 days at present.A large amount of uses of chemical pesticide, not only pollute human natural ecological environment of depending on for existence, slowed down the growth of fruits and vegetables and economic crops, influenced the quality of fruits and vegetables and economic crops, residual chemical pesticide in fruits and vegetables and economic crops is also greatly endangering the healthy of people.The technology of the herbal control disease of latest developments, because cost is higher, large tracts of land is used and has been subjected to certain restriction.Therefore, meeting nuisanceless, low-cost, green safety, the fruits and vegetables that meet the recycling economy principle and economic crops disease control production technology more and more is subject to people's attention.
The principle of fruits and vegetables and economic crops biological control of diseases technology mainly is to utilize various useful microorganisms; by interbiotic competition effect, antagonism, hyperparasitism, cross-protection and inducing plant disease resistance etc.; suppress the breeding and the activity of some disease; for plant provides a good growing environment; increase the disease resistance of plant self; can make plant avoid infecting and diffusion of pathogen, suppress the harm that disease produces plant.The biological control technology is to the person poultry safety, and pollution of ecological environment does not develop immunity to drugs, and only kills and wounds or suppresses controlling object, can participate in the regulation and control of ecotope, keeps the Nature ecological balance, plays the effect of long acting antibiotic.Can reduce the production cost of fruits and vegetables and economic crops again.
" fulvic acid bacterial manure " is meant according to my application number 200610162041.6 patents of invention in application on December 11st, 2006 among the present invention, patent name " pomace, stalk fermentation is produced the method for fulvic acid bacterial manure ", in this patent, a kind of pomace that utilizes is disclosed, all kinds of agricultural crop straws, excrement of animals, all kinds of waste from farm and sideline product processing such as granulated slag are raw material, after many bacterial classifications of microorganism solid-semi-solid mixed culture fermentation, the liquid fulvic acid bacterial manure of being produced, the general designation of semi-solid fulvic acid bacterial manure and solid fulvic acid bacterial manure also comprises the biochemical fulvic acid and the biochemical fulvic acid class bacterial manure that utilize additive method to produce.
Biochemical fulvic acid (BFA) is a microbial fermentation and generating, it is the very complicated mixture of a kind of composition, wherein contain in a large number bioactivator and nutritions such as the influential enzyme of growing process, amino acid, vitamin and trace elements, to promoting plant growing, growing remarkable efficacy is arranged.BFA has good water-solubility, in soil, can strengthen the buffer capacity of soil by complexing, chelating, suction-operated, improve guarantor's fertilizer, the water retention capacity of soil, improve the gas permeability of soil and the activity of edaphon, increase effective flora quantity and reach the effect of improving the soil.BFA is the material of finding at present of regulating plant leaf surface pore opening degree most effectively, it can dwindle blade face pore opening degree, reduce moisture transpiration, make plant and soil conservation more water, promote the root system development growth simultaneously, improve the root system vigor and make crop absorption more water and nutriment that the two complements each other, increase income and decrease expenditure, reach the purpose that improves crops resisting drought saving water ability.BFA is a kind of good plant growth regulator, has stronger ions binding ability, can significantly improve plant to trace elements absorbed and running ability.
Biochemical fulvic acid has quite tangible five big characteristics: the one, and the biochemical fulvic acid molecular weight is little, and infiltration capacity is strong, is utilized by bio-absorbable easily; The 2nd, good water solubility, resistance to hard water is strong, does not flocculate, and does not precipitate, and is easy to use; The 3rd, the activity group content height, physiologically active is big, and is strong to complexing of metal ion, sequestering power; The 4th, saturation solubility is big, with trace element good intermiscibility is arranged; The 5th, do not contain heavy metal and other harmful substance.The five big characteristics that it is exclusive have great biologically active to plant growing, can obviously improve immunologic function and the anti-adversity of plant, reach the effect of prevention and elimination of disease and pests, improve the soil salinization and alkalization.Therefore biochemical fulvic acid is that the best nutritional that is widely used in all kinds of crops is supplied with body.
" the biological antagonist microbial inoculum " that be used for fruits and vegetables and the integrated control of economic crops disease biological among the present invention is meant according to my application number 200810126385.0 patents of invention in application on June 30th, 2008, patent name " biological control method of diseases of greenhouse corps ", in this patent, disclose a kind of through bacillus subtilis (Bacillus subtilis) BS2-5, the BS6, the BS8-3 three strain antagonism bacterium that separate, screening obtains has the wide spectrum antagonism to fruits and vegetables and economic crops disease pathogen.
In my application number 200810126385.0 patents of invention, disclose bacillus subtilis (Bacillus subtilis) BS2-5, BS6, BS8-3 have been cultivated through series of process respectively, after three kinds of bacterium mix by a certain percentage, insert the fermentation tank liquid culture again, obtain to produce method with the biological antagonist microbial inoculum.Produce and all kinds of plant disease pathogens are had resistance of wide spectrum with the biological antagonist microbial inoculum.
The present invention is by biological antagonist microbial inoculum, both composite applications of fulvic acid bacterial manure, can remedy the deficiency of using separately to greatest extent, give full play to both advantages, it is the beneficial microbe colony that suppresses, kills pathogen of crop, can instead of chemical pesticide control corps diseases, reduce the effect of chemical fertilizer usage amount, reach the purpose that the agricultural product green safety is produced.
" biochemical fulvic acid foliage fertilizer " is meant according to my application number 200610162041.6 patents of invention in application on December 11st, 2006 among the present invention, patent name " method that pomace, stalk fermentation are produced fulvic acid bacterial manure ", in this patent, a kind of liquid fulvic acid bacterial manure production method is disclosed, nutritional need according to the different vegetative stages of all kinds of crops, with the composite nitrogenous fertilizer of liquid fulvic acid bacterial manure, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or trace element, the biochemical fulvic acid foliage fertilizer that is made into.
" biochemical fulvic acid dashes fertilising " is meant according to my application number 200610162041.6 patents of invention in application on December 11st, 2006 among the present invention, patent name " method that pomace, stalk fermentation are produced fulvic acid bacterial manure ", in this patent, a kind of semi-solid fulvic acid bacterial manure production method is disclosed, Different Nutrition demand according to all kinds of crops, with the composite nitrogenous fertilizer of semi-solid fulvic acid bacterial manure, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or rare trace elements, the biochemical fulvic acid that is made into dashes fertilising.
It is the direction of China's modernization agricultural development that the utilization ecological principle is built efficient ecological agricultural Model industrialization system engineering.This efficient ecological agricultural can solve the comprehensive utilization of plant husbandry and discarded object thereof preferably, make it organically integrate the formation biological chain, improve the product yield and the quality of plant husbandry greatly, keep eco-friendly, make and the agricultural economy cycle development bring huge economic benefit and social benefit.
Do not find by retrieval to invent identical prior art and patented invention with me.
Summary of the invention
The biological synthesis that the purpose of this invention is to provide a kind of fruits and vegetables and economic crops disease is prevented and treated method.The composite application on fruits and vegetables and economic crops by biological antagonist microbial inoculum and fulvic acid bacterial manure, give full play to both advantages, suppress, kill pathogen, with instead of chemical pesticide control corps diseases, reduce chemical fertilizer and use, reach the purpose that green safety is produced.
The present invention realizes by the following technical solutions:
1. composite application contains biological antagonist microbial inoculum and the fulvic acid bacterial manure of Bacillus subtillis (Bacillussubtilis) BS2-5, BS6, BS8-3 bacterium in the growth of fruits and vegetables and economic crops.
2. according to the demand of fruits and vegetables and economic crops shoot and leaf growth, after the biological antagonist microbial inoculum after the dilution and the biochemical fulvic acid foliage fertilizer for preparing mixed,, sprayed once at plant leaf surface every 10-20 days from plant germination.
3. use the biological antagonist microbial inoculum in fruits and vegetables and economic crops growth, according to the demand of fruits and vegetables and economic crops shoot and leaf growth, use biological antagonist microbial inoculum consumption 0.5-5 kilogram/mu each time, thin up 50-500 doubly.
4. applicating liquid fulvic acid bacterial manure in the growth of fruits and vegetables and economic crops, demand according to fruits and vegetables and economic crops shoot and leaf growth, applicating liquid fulvic acid bacterial manure usage amount 0.5-5.0 kilogram/mu each time, thin up 100-500 doubly, nutritional need according to all kinds of fruits and vegetables and the different vegetative stages of economic crops, composite nitrogenous fertilizer, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or trace element are made into the biochemical fulvic acid foliage fertilizer.
5. in fruits and vegetables and economic crops growth, use solid or semi-solid fulvic acid bacterial manure, amount of application 20-50 kilogram/mu, thin up 200-500 doubly, nutritional need according to the soil situation, composite nitrogenous fertilizer, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or rare trace elements, be made into biochemical fulvic acid and dash fertilising, after fruits and vegetables and economic crops blade face spray biological antagonist microbial inoculum and biochemical fulvic acid foliage fertilizer for the first time, dashed at fruits and vegetables and economic crops root and execute or root is irritated biochemical fulvic acid and dashed fertilising the same day, can reach inhibition, kill the effect of plant leaf surface and pathogen of crop on every side.
The used bacterial classification of the present invention all is known bacterial classification, can obtain by known screening, commercial means or other approach.
The invention has the advantages that:
1. according to my application number 200810126385.0 patents of invention in application on June 30th, 2008, disclosed a kind of in the patent name " biological control method of diseases of greenhouse corps " through separating, bacillus subtilis (Bacillus subtilis) BS2-5 that fruits and vegetables and economic crops disease pathogen are had the wide spectrum antagonism that screening obtains, BS6, BS8-3 three strain antagonism bacterium and application number 200610162041.6 patents of invention of applying on December 11st, 2006 according to me, patent name " pomace, stalk fermentation is produced the method for fulvic acid bacterial manure ", disclose and a kind ofly utilized all kinds of waste from farm and sideline product processing to be raw material, the liquid fulvic acid bacterial manure of being produced, the method of semi-solid fulvic acid bacterial manure and solid fulvic acid bacterial manure, the present invention is in conjunction with my two patents of invention, characteristics according to fruits and vegetables and economic crops growth, be applied in the biological synthesis control of fruits and vegetables and economic crops disease, be a kind of meet nuisanceless, low-cost, green safety, the fruits and vegetables and the economic crops production technology that meet recycling economy.
2. operating procedure of the present invention is simple, and is harmless to user's health.
3, the present invention has improved fruits and vegetables and economic crops quality and output, and makes it meet outlet fruits and vegetables and economic crops not have the residual requirement of chemical pesticide.
4, the present invention compares with present general chemical pesticide control method, and production cost descends significantly, and is then very remarkable to the disease resisting effect of fruits and vegetables and economic crops.
5. the present invention is in the control of fruits and vegetables and economic crops disease, and compound use biological antagonist microbial inoculum and fulvic acid bacterial manure reach complete instead of chemical agricultural chemicals, reduces or substituted chemistry fertilizer, has realized the production process of the agricultural product of nuisanceless, green safety.
Embodiment
Among the embodiment, experimental group is meant the orchard of taking this patent method to test production; Control group is meant the orchard of testing production with the chemical pesticide control method.
Example I:
The method of the preventing and treating comparative trial technical process of pear tree disease:
One, test period and place
1, test period: at the beginning of 2008 5 months～in by the end of September, 2008.
2, test site:
(1) Pucheng County Jing Yao town height village participates in test peasant household 9 families, 40 mu of pear tree test area.
(2) Yao Bao village, Pucheng County Dong Chen town participates in test peasant household 8 families, 30 mu of pear tree test area.
Two, materials and methods
1, experimental group is used medicament:
(1) biological antagonist microbial inoculum: brown liquid, 6,000,000,000/milliliter of viable counts;
(2) fulvic acid bacterial manure:
Liquid biochemical fulvic acid fertilizer: yellow liquid, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
Solid～semi-solid biochemical fulvic acid fertilizer: light yellow paste, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
2, control group is used medicament:
(1) 70% thiophanate methyl WP, Japanese Cao Da company produces, and is commercially available.
(2) 12.5% alkene azoles alcohol WP, Jiangsu Jiannong Agrochemical Co., Ltd. produces, and is commercially available.
(3) 3% polyoxin WP, Beijing Da Bei farming group produces, and is commercially available.
3, trial crops: Dangshan pear.
Three, test method:
(1) experimental group test method:
According to the demand of Dangshan pear vegetative stage, when leaf just germinates, use 0.5～1 kilogram/mu of biological antagonist microbial inoculum consumption for the first time, dilute 50～200 times,, use biological antagonist microbial inoculum consumption and increase gradually along with branches and leaves are grown up gradually, reach 4～5 kilograms/mu at last, dilute 200～500 times.
Equally, liquid biochemical fulvic acid fertilizer is when leaf just germinates, and 0.5～1 kilogram/mu of amount of application dilutes 100～200 times, and along with branches and leaves are grown up gradually, amount of application reaches 4～5 kilograms/mu at last, dilutes 200～500 times; Mix the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back, adopts power driven sprayer to spray on the blade face; Sprayed once in per approximately 10～20 days, this test period sprays 9 times altogether.
10～50 kilograms/mu of solids-semi-solid fulvic acid bacterial manure amount of application, dilute 100～500 times, nutritional need according to the soil situation, composite nitrogenous fertilizer, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or rare trace elements, be made into biochemical fulvic acid and dash fertilising, at the beginning of 5 months, when spraying biological antagonist microbial inoculum and liquid biochemical fulvic acid fertilizer mixed liquor first, irritated once at the Dangshan pear tree root same day, to reach inhibitions, to kill the effect of Dangshan pear blade face and Dangshan pear tree pathogen of crop on every side.。
(2) control group test method:
Use chemical bactericide routinely, successively use 70% thiophanate methyl WP, 12.5% alkene azoles alcohol WP, three kinds of medicaments of 3% polyoxin WP.Sprayed once in per approximately 15 days, and sprayed altogether 9 times, adopt power driven sprayer to spray.
Four, test test item and analysis thereof
1, Dangshan pear leaf chlorophyll horizontal detection
Detection time: detect altogether in process of the test three times, the time is respectively: on June 10th, 2008, July 5, August 22.
Detecting instrument: YLS-518 chlorophyll measuring instrument.
The experimental group and the control group pears chlorophyll content in leaf blades table of comparisons
| Detection time and test item | Chlorophyll content index on June 10 (mean value) | Chlorophyll content index on July 5 (mean value) | Chlorophyll content index on August 22 (mean value) |
| Experimental group | 66.5 | 67.1 | 66.8 |
| Control group | 56.4 | 57.1 | 57.4 |
| Experimental group is than control group chlorophyll index | 17.9％ | 17.5％ | 16.4％ |
| Raising ratio % |
Three groups are detected digital average, and biological control experimental group chlorophyll index (mean value) is higher than chemical control control group 17.26%; From outside watch, biological control experimental group pears blade is thick, big, green, the tree vigo(u)r well-grown; Chemical control control group pears blade has the disease vestige, and growing way is relatively poor.
2, pear tree blade antagonistic microbe quantity detects:
Adopt dilution-plate method that the pear tree blade is carried out three times in test period and detect, effective antagonism bacterium number is 10 on the biological control experimental group pear tree blade
9-10
10/ cm
2The chemical control control group does not detect effective antagonism bacterium.
3, state of an illness statistics
After having sprayed last medicine, carry out the control efficiency investigation in by the end of September, 2008, press grade scale investigation test region morbidity strain number, calculate the incidence of disease, disease index and control efficiency.Any disease does not take place in biological control experimental group pears blade, control group generation pears black spot, the incidence of disease 12%.
4, fruit evaluation: the biological control experimental group is compared with the chemical control control group, the bright and clean immaculate of fruit surface, and sugariness and mouthfeel obviously are better than control group.
5. output: pears output is increased production more than 10%.
Five, Test Summary
Pears black heart, rust of pear and pears black spot are ubiquitous diseases during the crisp pears in Pucheng County are produced in recent years, orchard test card open-birth thing antagonism microbial inoculum and fulvic acid bacterial manure composite application, synergy, can prevent and treat this three big main disease of pear tree effectively, simultaneously can improve the pears chlorophyll content in leaf blades, strengthen tree system disease resistance and photosynthesis ability, both can increase output, can improve fruit quality again.
Example II:
The method of the preventing and treating comparative trial technical process of grape disease
One, test period and place
1, test period: at the beginning of 2008 5 months～in early October, 2008 is only.
2, test site
Too peaceful village, Shang Wang township, Pucheng County participates in test peasant household 6 families, participates in 40 mu of test area.
Two, materials and methods
1, experimental group is used medicament:
(1) biological antagonist microbial inoculum: brown liquid, 6,000,000,000/milliliter of viable counts.
(2) fulvic acid bacterial manure:
Liquid fulvic acid bacterial manure: yellow liquid, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.Solid-semi-solid fulvic acid bacterial manure: light yellow paste, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
2, control group is used medicament
(1) Difenoconazole WP, Bayer A.G produces, and is commercially available.
(2) 12.5% alkene azoles alcohol WP, Jiangsu Jiannong Agrochemical Co., Ltd. produces, and is commercially available.
(3) alkene acyl Malin WP, Beijing Da Bei farming group produces, and is commercially available.
3, trial crops:
Red grape
4, test method:
(1) biological control experimental group test method:
Demand according to the red grape vegetative stage, when leaf just germinates, use for the first time 1～2 kilogram/mu of biological antagonist microbial inoculum consumption, dilute 50～200 times, along with the red grape leaf is grown up gradually, use biological antagonist microbial inoculum consumption and increase gradually, reach 4～5 kilograms/mu at last, dilute 200～500 times.
Equally, liquid biochemical fulvic acid fertilizer is when the red grape leaf has just germinateed, and 0.5～1 kilogram/mu of amount of application dilutes 100～200 times, and along with leaf is grown up gradually, amount of application reaches 4～5 kilograms/mu at last, dilutes 200～500 times; Mix the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back, adopts power driven sprayer to spray on the blade face; Began to end to early October at the beginning of 5 months, sprayed once in per approximately 10～20 days, this test period sprays 10 times altogether.
10～50 kilograms/mu of solids-semi-solid fulvic acid bacterial manure amount of application, dilute 100～500 times, according to the nutritional need of soil situation, composite nitrogenous fertilizer, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or rare trace elements are made into biochemical fulvic acid and dash fertilising; At the beginning of 5 months, on the same day that sprays biological antagonist microbial inoculum and liquid biochemical fulvic acid foliage fertilizer mixed liquor for the first time, execute and spread manuer in holes once at red grape Teng Genbuchong, can reach inhibition, kill the effect of the pathogen of crop around red grape blade face and the red grape tendril.
(2) chemical control control group:
Began to end to early October at the beginning of 5 months, the chemical bactericide of Shi Yonging successively uses Difenoconazole WP routinely, 12.5% alkene azoles alcohol WP, and three kinds of medicaments of alkene acyl Malin WP sprayed once in per approximately 7 days.The employing power driven sprayer sprays.
Four, test test item and analysis thereof
1, red grape leaf chlorophyll horizontal detection result
Detection time: on June 10th, 2008, July 5, August 22, in process of the test
In detect altogether three times.
Detecting instrument: YLS-518 chlorophyll measuring instrument
The experimental group and the control group red grape chlorophyll content in leaf blades table of comparisons
| Detection time and project | Chlorophyll content index on June 10 (mean value) | Chlorophyll content index on July 5 (mean value) | Chlorophyll content index on August 22 (mean value) |
| Experimental group | 43.1 | 39.5 | 41.1 |
| Control group | 37.3 | 32.8 | 31.9 |
| Experimental group improves ratio % than control group chlorophyll index | 15.54％ | 20.42％ | 28.84％ |
Biological control experimental group red grape leaf chlorophyll index (mean value) is higher by 21.27% than control group, and grape leave is thick, big, green, and grape vine is overgrow long good, obviously is better than the chemical control control group.
2, grape leave antagonistic microbe quantity testing result
Adopt dilution-plate method that grape leave is carried out three times in test period and detect, effective antagonism bacterium bacterium several 10 on the experimental group grape leave
9-10
10/ cm
2, control group does not detect effective antagonism bacterium.
3, state of an illness statistics
After having sprayed last medicine, carry out the control efficiency investigation in by the end of September, 2008, press grade scale investigation sub-district morbidity strain number, calculate the incidence of disease, disease index and control efficiency.Any disease does not take place in biological control experimental group grape leave, control group generation white rot and downy mildew, and the incidence of disease is respectively 12%, 17.6%
4, fruit evaluation
The heavy 8g of chemical control control group grape simple grain, pol is 14%, the fruit grain is dark red, tarnish; The heavy 10g of biological control experimental group grape simple grain, fruit grain color and luster bright red, light, pol 18%, mouthfeel obviously are better than control group.
5, output
3100 jin/mu of biological control experimental group average yields, 2800 jin/mu of control group average yields, biological control group per mu yield improves 10%, directly revenue growth about 15%.
Five, Test Summary
White rot, downy mildew, sour rot, anthracnose are ubiquitous four big diseases during red grape is produced in recent years.Orchard test card open-birth thing antagonism microbial inoculum and fulvic acid bacterial manure composite application, synergy, can prevent and treat the main disease of grape effectively, simultaneously can improve the grape leave chlorophyll content, strengthen grape self systemic disease resistance and photosynthesis ability, both output can be increased, and fruit quality can be improved again.
EXAMPLE III:
The method of the preventing and treating comparative trial technical process of kiwi fruit disease
One, test period and place
1, test period: end year mid-September in May, 2008-2008.
2, test site
Yao Si village, capital town, Mei County participates in test peasant household 11 families, participates in 30 mu of test area.
Two, materials and methods
1, experimental group is used medicament
(1) biological antagonist microbial inoculum: brown liquid, 6,000,000,000/milliliter of viable counts.
(2) fulvic acid bacterial manure:
Liquid fulvic acid bacterial manure: yellow liquid, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
Solid-semi-solid fulvic acid bacterial manure: light yellow paste, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
2, control group is used medicament:
(1) Difenoconazole WP, Bayer A.G produces, and is commercially available.
(2) 12.5% alkene azoles alcohol WP, Jiangsu Jiannong Agrochemical Co., Ltd. produces, and is commercially available.
(3) alkene acyl Malin WP, Beijing Da Bei farming group produces, and is commercially available.
3, trial crops: kiwi fruit
4, test method:
(1) biological control experimental group test method:
When the kiwi fruit leaf has just germinateed, use 1～2 kilogram/mu of biological antagonist microbial inoculum consumption for the first time, dilute 50～200 times, along with the kiwi fruit leaf is grown up gradually, use biological antagonist microbial inoculum consumption and increase gradually, reach 4～5 kilograms/mu at last, dilute 200～500 times.
Equally, liquid biochemical fulvic acid fertilizer is when the kiwi fruit leaf has just germinateed, and 0.5～1 kilogram/mu of amount of application dilutes 100～200 times, and along with leaf is grown up gradually, amount of application reaches 4～5 kilograms/mu at last, dilutes 200～500 times; Mix the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back, adopts power driven sprayer to spray on the blade face; Began to end to mid-September at the beginning of 5 months, sprayed once in per approximately 10～20 days, this test period sprays 9 times altogether.
10～50 kilograms/mu of solids-semi-solid fulvic acid bacterial manure amount of application, dilute 100～500 times, according to the nutritional need of soil situation, composite nitrogenous fertilizer, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or rare trace elements are made into biochemical fulvic acid and dash fertilising; At the beginning of 5 months, on the same day that sprays biological antagonist microbial inoculum and liquid biochemical fulvic acid foliage fertilizer mixed liquor for the first time, dash to execute at the kiwi fruit usage tree root and irritate once, can reach inhibitions, kill the effect of kiwi fruit blade face and macaque peach pathogen of crop on every side with root.
(2) chemical control experimental group:
At the beginning of 5 months, begin to end, use chemical bactericide routinely, successively use Difenoconazole WP, 12.5% alkene azoles alcohol WP, three kinds of medicaments of alkene acyl Malin WP to mid-September.Sprayed once in per approximately 7 days.The employing power driven sprayer sprays.
Three, result of the test
1, leaf chlorophyll horizontal detection result
On June 10th, 2008, July 5, August 22, adopt YLS-518 chlorophyll measuring instrument, measure biological control experimental group and control group kiwi fruit blade chlorophyll content, biological control experimental group kiwi fruit blade chlorophyll content is higher by 13% than control group, blade is thick, big, green, the tree vigo(u)r well-grown obviously is better than the chemical control group.
2, blade antagonistic microbe quantity testing result
Adopt dilution-plate method that blade is carried out three times in test period and detect, effective antagonism bacterium bacterium number reaches 10 on the experiment blade
10/ cm
2. control group does not detect effective antagonism bacterium.
3, state of an illness statistics
After having sprayed last medicine, carry out the control efficiency investigation, press grade scale investigation sub-district morbidity strain number, calculate the incidence of disease, disease index and control efficiency.Any disease does not take place in biological control group blade, control group generation kiwi berry bacterial canker, kiwi fruit leaf rot, kiwi fruit brown spot, and the incidence of disease is respectively 11%, 16%, 9%.
4, fruit evaluation
Compare with the chemical control experimental group, biological control experimental group kiwi fruit pol improves 8.7%, and Vc content improves 33.5%, and soluble solid content improves 8.2%, excellent taste.
5, output
Compare with control group, biological control experimental group per mu yield improves 14%.
Four, Test Summary
Kiwi berry bacterial canker, kiwi fruit leaf rot, kiwi fruit brown spot are ubiquitous diseases during kiwi fruit is produced in recent years.Orchard test card open-birth thing antagonism microbial inoculum and fulvic acid bacterial manure composite application, synergy, can prevent and treat the main disease of kiwi fruit effectively, simultaneously can improve chlorophyll content in leaf blades, strengthen kiwi fruit self systemic disease resistance and photosynthesis ability, both output can be increased, and fruit quality can be improved again.
EXAMPLE IV:
The method of the preventing and treating comparative trial technical process of Chinese cabbage disease
One, test period and place
1, test period: in May, 2008-2008 year early September ends.
2, test site
Xianyang participates in test peasant household 7 families, participates in 20 mu of test area.
Two, materials and methods
1, experimental group is used medicament
(1) biological antagonist microbial inoculum: brown liquid, 6,000,000,000/milliliter of viable counts.
(2) fulvic acid bacterial manure:
Liquid fulvic acid bacterial manure: yellow liquid, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
Solid～semi-solid fulvic acid bacterial manure: light yellow paste, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
2, control group is used medicament
500 times of liquid of virus of A wetting powder, virus is peaceful, 5% bacterium disappears, 25% metalaxyl, 50% tmtd, 4000 times of liquid of agricultural streptomycin, new 4000 times of liquid of phytomycin, and above medicament all has commercially available.
3, trial crops: Chinese cabbage.
4, test method:
(1) biological control experimental group test method:
According to the demand of Chinese cabbage vegetative stage, when leaf just germinates, use 1～1.5 kilogram/mu of biological antagonist microbial inoculum consumption for the first time, dilute 50～200 times,, use biological antagonist microbial inoculum consumption and increase gradually along with leaf is grown up gradually, reach 4.5～5 kilograms/mu at last, dilute 200～500 times.
Equally, liquid biochemical fulvic acid fertilizer is when the Chinese cabbage leaf has just germinateed, and 1～1.5 kilogram/mu of amount of application dilutes 100～200 times, and along with leaf is grown up gradually, amount of application reaches 4～5 kilograms/mu at last, dilutes 200～500 times; Mix the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back, adopts power driven sprayer to spray on the blade face; Began to end to early September at the beginning of 5 months, sprayed once in per approximately 15 days, this test period sprays 9 times altogether.
10～50 kilograms/mu of solids-semi-solid fulvic acid bacterial manure amount of application, dilute 100～500 times, nutritional need according to the soil situation, composite nitrogenous fertilizer, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or rare trace elements, be made into biochemical fulvic acid and dash fertilising, at the beginning of 5 months, spray for the first time the same day of the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back mixed liquor, dash at the Chinese cabbage root and to execute biochemical fulvic acid and dash fertilising once, can reach inhibitions, kill the effect of Chinese cabbage blade face and Chinese cabbage pathogen of crop on every side.
(2) chemical control control group:
At the beginning of 5 months, begin to end to mid-September, use the chemical sterilization agent method routinely, successively use 500 times of liquid of virus of A wetting powder, 50% tmtd, 4000 times of liquid of agricultural streptomycin, new 4000 times of liquid of phytomycin, medicaments such as virus is peaceful, 5% bacterium disappears, 25% metalaxyl, 50% tmtd, sprayed once in every approximately 7-15 days, and adopted power driven sprayer to spray.
Three, result of the test
1, leaf chlorophyll horizontal detection result
In test period, adopt YLS-518 chlorophyll measuring instrument, Chinese cabbage leaf chlorophyll content has been carried out three times altogether detected, biological control experimental group Chinese cabbage leaf chlorophyll index (mean value) is higher by 11% than chemical control control group, the Chinese cabbage growth impetus is good, and mouthfeel obviously is better than the chemical control experimental group.
2, blade antagonistic microbe quantity testing result
In test period, adopt dilution-plate method that blade is carried out three times and detect, effective antagonism bacterium bacterium number reaches 10 on the experiment blade
9～10
10/ cm
2, control group does not detect effective antagonism bacterium.
3, state of an illness statistics
Press grade scale investigation sub-district morbidity strain number, calculate the incidence of disease, disease index and control efficiency.Any disease does not take place in the biological control experimental group, and Chinese cabbage virus disease, downy mildew and soft rot take place control group, and the incidence of disease is respectively 13%, 9% and 11%.
4, output
Compare with control group, biological control experimental group per mu yield improves 15%.
Four, Test Summary
Chinese cabbage virus disease, downy mildew and soft rot are three big diseases during Chinese cabbage produces, and have a strong impact on ordinary production, lose huge.Field experiment shows, biological antagonist microbial inoculum and fulvic acid bacterial manure composite application, synergy, can prevent and treat three big diseases in the Chinese cabbage production effectively, simultaneously can improve chlorophyll content in leaf blades, strengthen Chinese cabbage self systemic disease resistance and photosynthesis ability, both can increase output, can improve quality of vegetable again.
EXAMPLE V:
The method of the preventing and treating comparative trial technical process of disease for tea plant
One, test period and place
1, test period: in early October ,-2008 ends at the beginning of 2008 3 months.
2, test site
Shan Nan Hanzhong participates in test peasant household 7 families, participates in 40 mu of test area.
Two, materials and methods
1, experimental group is used medicament
(1) biological antagonist microbial inoculum: brown liquid, 6,000,000,000/milliliter of viable counts.
(2) fulvic acid bacterial manure:
Liquid fulvic acid bacterial manure: yellow liquid, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
Solid～semi-solid fulvic acid bacterial manure: light yellow paste, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
2, control group is used medicament
70% thiophanate methyl, 75-100g/ mu, 75% tpn, 95-125g/ mu, 500 times of liquid of 50% carbendazim, 800-1000 times of liquid of 50% dimethachlon emulsion, above medicament all has commercially available.
3, trial crops: tea tree.
4, test method:
(1) biological control experimental group test method:
Demand according to the growth of tea plant stage, when leaf just germinates, use for the first time 0.5～1 kilogram/mu of biological antagonist microbial inoculum consumption, dilute 50～200 times, along with the tea tree leaf is grown up gradually, use biological antagonist microbial inoculum consumption and increase gradually, reach 3.5～5 kilograms/mu at last, dilute 200～500 times.
Equally, liquid biochemical fulvic acid fertilizer is when the tea tree leaf has just germinateed, and 1～1.5 kilogram/mu of amount of application dilutes 100～200 times, and along with leaf is grown up gradually, amount of application reaches 4～5 kilograms/mu at last, dilutes 200～500 times; Mix the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back, adopts power driven sprayer to spray on the blade face; Began to end to early October at the beginning of 3 months, sprayed once in per approximately 15 days, this test period sprays 14 times altogether.
10～50 kilograms/mu of solids-semi-solid fulvic acid bacterial manure amount of application, dilute 100～500 times, nutritional need according to the soil situation, composite nitrogenous fertilizer, phosphate fertilizer, all kinds of nutritive elements of potash fertilizer or interpolation macroelement or rare trace elements, be made into biochemical fulvic acid and dash fertilising, at the beginning of 3 months, spray for the first time the same day of the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back mixed liquor, dash at the tea tree root and to execute biochemical fulvic acid and dash fertilising once, can reach inhibitions, kill the effect of tea tree blade face and tea tree pathogen of crop on every side; In May,, dash fertilising once towards executing biochemical fulvic acid at the tea tree root according to same amount of application and dilution ratio.
(2) chemical control control group:
At the beginning of 3 months, begin to end to mid-September, use the chemical sterilization agent method routinely, successively use 70% thiophanate methyl, 70～100g/ mu, 75% tpn, 95～130g/ mu, 500 times of liquid of 50% carbendazim, 800-1000 times of liquid of 50% dimethachlon emulsion sprayed once in every approximately 7-15 days, adopted power driven sprayer to spray.
Three, result of the test
1, leaf chlorophyll horizontal detection result
In test period, adopt YLS-518 chlorophyll measuring instrument, the tea leaf chlorophyll content has been carried out three times altogether to be detected, biological control experimental group tea free chlorophyll index (mean value) is higher by 14% than chemical control control group, the growth of tea plant impetus is good, and the tealeaves mouthfeel obviously is better than the chemical control experimental group.
2, blade antagonistic microbe quantity testing result
In test period, adopt dilution-plate method that blade is carried out three times and detect, effective antagonism bacterium bacterium number reaches 10 on the experiment blade
9～10
10/ cm
2, control group does not detect effective antagonism bacterium.
3, state of an illness statistics
Press grade scale investigation sub-district morbidity strain number, calculate the incidence of disease, disease index and control efficiency.Any disease does not take place in the biological control experimental group, control group generation tea shoot southern blight, tea white star and tea black rot, and the incidence of disease is respectively 10%, 9.7% and 12%.
4, output
Compare with control group, biological control experimental group per mu yield improves 13%.
Four, Test Summary
Tea shoot southern blight, tea white star and tea black rot are three big diseases during tea tree produces, and have a strong impact on ordinary production, lose huge.Field experiment shows, biological antagonist microbial inoculum and fulvic acid bacterial manure composite application, synergy, can prevent and treat the disease in the Tea Production effectively, simultaneously can improve chlorophyll content in leaf blades, strengthen tea tree self systemic disease resistance and photosynthesis ability, both can increase output, can improve tea leaf quality again.
Example VI:
The method of the preventing and treating comparative trial technical process of FLOS CHRYSANTHEMI ALBA from Haizhou of China disease
One, test period and place
1, test period: in early November ,-2008 ends at the beginning of 2008 3 months.
2, test site
The Tongxiang, Hangzhou participates in test peasant household 5 families, participates in 18 mu of test area.
Two, materials and methods
1, experimental group is used medicament
(1) biological antagonist microbial inoculum: brown liquid, 6,000,000,000/milliliter of viable counts.
(2) fulvic acid bacterial manure:
Liquid fulvic acid bacterial manure: yellow liquid, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
Solid～semi-solid fulvic acid bacterial manure: light yellow paste, 0.5 hundred million/milliliter of viable counts, NPK ≧ 4%.
2, control group is used medicament
The spraying control is commercially available after 300 times of dilutions of 5% jinggangmycin.
3, trial crops: FLOS CHRYSANTHEMI ALBA from Haizhou of China.
4, test method:
(1) biological control experimental group test method:
Demand according to the FLOS CHRYSANTHEMI ALBA from Haizhou of China vegetative stage, when branches and leaves just germinate, use for the first time 0.5～1 kilogram/mu of biological antagonist microbial inoculum consumption, dilute 50～200 times, along with the FLOS CHRYSANTHEMI ALBA from Haizhou of China branches and leaves are grown up gradually, use biological antagonist microbial inoculum consumption and increase gradually, at FLOS CHRYSANTHEMI ALBA from Haizhou of China when a bud just ready to burst, reach 3.5～5 kilograms/mu, dilute 200～500 times.
Equally, liquid biochemical fulvic acid fertilizer when the FLOS CHRYSANTHEMI ALBA from Haizhou of China branches and leaves have just germinateed, 1～1.5 kilogram/mu of amount of application, dilute 100～200 times, along with branches and leaves are grown up gradually, amount of application increases gradually, when a bud just ready to burst, reach 4～5 kilograms/mu at FLOS CHRYSANTHEMI ALBA from Haizhou of China, dilute 200～500 times; Mix the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back, adopts power driven sprayer to spray on the blade face; Began to end to early November at the beginning of 3 months, sprayed once in per approximately 15 days, this test period sprays 15 times altogether.
10～50 kilograms/mu of solids-semi-solid fulvic acid bacterial manure amount of application, dilute 100～500 times, according to nutritional need at FLOS CHRYSANTHEMI ALBA from Haizhou of China, composite nitrogenous fertilizer nutritive element or rare trace elements, be made into biochemical fulvic acid and dash fertilising, at the beginning of 3 months, spray for the first time the same day of the fertile dilution of biological antagonist microbial inoculum and liquid biochemical fulvic acid back mixed liquor, dash at the FLOS CHRYSANTHEMI ALBA from Haizhou of China root and to execute biochemical fulvic acid and dash fertilising once, can reach inhibitions, kill the effect of FLOS CHRYSANTHEMI ALBA from Haizhou of China blade face and FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight pathogen on every side; In May,, dash fertilising once towards executing biochemical fulvic acid at the FLOS CHRYSANTHEMI ALBA from Haizhou of China root according to same amount of application and dilution ratio.
(2) chemical control control group:
Began to end to early November at the beginning of 3 months, use the chemical sterilization agent method routinely, finding that leaf blight begins, the spraying control sprayed once in every approximately 7-15 days after 300 times of dilutions of 5% jinggangmycin, adopted power driven sprayer to spray.
Three, result of the test
1, leaf chlorophyll horizontal detection result
In test period, adopt YLS-518 chlorophyll measuring instrument, the FLOS CHRYSANTHEMI ALBA from Haizhou of China chlorophyll content in leaf blades has been carried out three times altogether to be detected, biological control experimental group FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf chlorophyll index (mean value) is higher by 10% than chemical control control group, and the FLOS CHRYSANTHEMI ALBA from Haizhou of China growth impetus is good, and the fragrance of flowers is strong for FLOS CHRYSANTHEMI ALBA from Haizhou of China, flower flower shape is good, the flower size evenly, petal is thick, look beautiful is white, and pistil is deep yellow, and FLOS CHRYSANTHEMI ALBA from Haizhou of China drink mouthfeel obviously is better than the chemical control experimental group.
2, blade antagonistic microbe quantity testing result
In test period, adopt dilution-plate method that blade is carried out three times and detect, effective antagonism bacterium bacterium number reaches 10 on the experiment blade
9～10
10/ cm
2, control group does not detect effective antagonism bacterium.
3, state of an illness statistics
Press grade scale investigation sub-district morbidity strain number, calculate the incidence of disease, disease index and control efficiency.Any disease does not take place in the biological control experimental group, control group generation leaf blight, and the incidence of disease is 10%.
4, output
Compare with control group, biological control experimental group per mu yield improves 12%.
Four, Test Summary
Leaf blight is the main disease in the FLOS CHRYSANTHEMI ALBA from Haizhou of China growth, has a strong impact on ordinary production, loses huge.Field experiment shows, biological antagonist microbial inoculum and fulvic acid bacterial manure composite application, synergy, can prevent and treat the disease of leaf blight in the FLOS CHRYSANTHEMI ALBA from Haizhou of China growth effectively, simultaneously can improve chlorophyll content in leaf blades, strengthen FLOS CHRYSANTHEMI ALBA from Haizhou of China self systemic disease resistance and photosynthesis ability, both can increase output, can improve the FLOS CHRYSANTHEMI ALBA from Haizhou of China quality again.
The present invention is by the biological antagonist microbial inoculum; the composite use of both science of fulvic acid bacterial manure; can remedy the deficiency of using separately to greatest extent; give full play to the huge advantage of both compound uses; for solving the water resource shortage that present China agricultural faces; agricultural chemicals; the water pollution that the excessive use of chemical fertilizer brings; plough and degenerate; fruits and vegetables and economic crops are with low quality; damage by disease and insect spreads; serious problems such as the deterioration of the ecological environment; show multi-functional, high efficiency, advantage such as applicability is wide; solution to the problems referred to above has material base and real operability widely; therefore, its still not a kind of green technology that meets environmental protection and ecological principle also is a kind of novel biocontrol technology of the sustainable development ecological agriculture.
Produce and use the biological antagonist microbial inoculum, the comprehensive use of fulvic acid bacterial manure on crops, can carry out disease control with biological antagonist microbial inoculum instead of chemical agricultural chemicals fully, with fulvic acid bacterial manure part instead of chemical fertilizer, for fruits and vegetables and economic crops provide all kinds of nutrient components that are easy to absorb, strengthen fruits and vegetables and economic crops self growing ability and to the resistance against diseases of damage by disease and insect, improve the ecotope of fruits and vegetables and economic crops growth, reach the purpose of biological control, set up environmental protection, nuisanceless, sustainable development, the agricultural product production system of higher economic benefit.At home and abroad all there is the extremely strong market competitiveness in market, is subjected to the consumer and welcomes, and has good economic benefit and social benefit.
Claims (6)
1. the biological synthesis of fruits and vegetables and economic crops disease is prevented and treated method, it is characterized in that in fruits and vegetables and economic crops process of growth composite application contains biological antagonist microbial inoculum and the fulvic acid bacterial manure of Bacillus subtillis (Bacillus subtilis) BS2-5, BS6, BS8-3 bacterium.
2. the biological synthesis of fruits and vegetables according to claim 1 and economic crops disease is prevented and treated method, it is characterized in that demand according to fruits and vegetables and economic crops shoot and leaf growth, after biological antagonist microbial inoculum after the dilution and the biochemical fulvic acid foliage fertilizer for preparing mixed, from plant germination, sprayed once at plant leaf surface every 10-20 days.
3, the biological synthesis of fruits and vegetables according to claim 1 and economic crops disease is prevented and treated method, it is characterized in that after fruits and vegetables and economic crops blade face spray biological antagonist microbial inoculum and biochemical fulvic acid foliage fertilizer for the first time dashed at fruits and vegetables and economic crops root and execute or root is irritated biochemical fulvic acid and dashed fertilising the same day.
4. prevent and treat method according to the biological synthesis of claim 1,2 described fruits and vegetables and economic crops disease, it is characterized in that the demand according to the fruits and vegetables vegetative stage, use biological antagonist microbial inoculum consumption 0.5-5 kilogram/mu each time, thin up 50-500 doubly.
5. prevent and treat method according to the biological synthesis of claim 1,2 described fruits and vegetables and economic crops disease, it is characterized in that demand according to fruits and vegetables and economic crops shoot and leaf growth, applicating liquid fulvic acid bacterial manure usage amount 0.5-5.0 kilogram/mu each time, thin up 100-500 are doubly.
。6, the biological synthesis of fruits and vegetables according to claim 1 and economic crops disease is prevented and treated method, it is characterized in that using solid or semi-solid fulvic acid bacterial manure, and amount of application 20-50 kilogram/mu, thin up 200-500 are doubly.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904751A (en) * | 2015-04-09 | 2015-09-16 | 河南工业大学 | Wheat scab liquid biocontrol agent and preparation method thereof |
| CN104969803A (en) * | 2015-07-03 | 2015-10-14 | 广西壮族自治区农业科学院微生物研究所 | Cultivation method for preventing banana vasicular wilt from spreading among plantations |
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2009
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904751A (en) * | 2015-04-09 | 2015-09-16 | 河南工业大学 | Wheat scab liquid biocontrol agent and preparation method thereof |
| CN104904751B (en) * | 2015-04-09 | 2018-03-06 | 河南工业大学 | A kind of wheat scab liquid biocontrol agent and preparation method thereof |
| CN104969803A (en) * | 2015-07-03 | 2015-10-14 | 广西壮族自治区农业科学院微生物研究所 | Cultivation method for preventing banana vasicular wilt from spreading among plantations |
| CN104969803B (en) * | 2015-07-03 | 2017-06-23 | 广西壮族自治区农业科学院微生物研究所 | A kind of cultural method for controlling banana blight to be propagated between the border of garden |
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