CN101435754A - Magnetic nano marker concentrating apparatus and uses thereof - Google Patents
Magnetic nano marker concentrating apparatus and uses thereof Download PDFInfo
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- CN101435754A CN101435754A CNA2008102446844A CN200810244684A CN101435754A CN 101435754 A CN101435754 A CN 101435754A CN A2008102446844 A CNA2008102446844 A CN A2008102446844A CN 200810244684 A CN200810244684 A CN 200810244684A CN 101435754 A CN101435754 A CN 101435754A
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Abstract
The invention provides a magnetic nano-marker concentration device and application thereof, which belong to the technical field of immunity chromatography analysis. The device comprises a sample feeding tank, a reclaiming tank, a collecting pipe, high-strength magnets and a liquor pump, wherein the sample feeding tank is used for feed samples to be tested; the reclaiming tank is used for reclaiming the samples; the collecting pipe is used for carrying a concentrated magnetic nano-marker; the high-strength magnets surround on the periphery of the collecting pipe; the liquor pump is used for driving the samples to be tested; various parts are connected through conduits; a metal tube capable of shielding a magnetic field is connected between the liquor pump and the collecting pipe so as to prevent magnetic microspheres from being absorbed on the inner wall of the conduits through ferromagnetic effect. In the invention, the magnetic nano microspheres as the testing markers of carriers are absorbed on the inner wall of the collecting pipe through the ferromagnetic effect of the high-strength magnets, so that the aim of concentrating trace testing markers in the samples to be tested can be achieved. The magnetic nano-marker concentration device is combined with magnetic immunity chromatography, has the advantages of simple mechanism, convenient operation, low cost, high efficiency and the like, and can be widely applied in biology, medicine, research, food, environment, quarantine, anti-terrorism anti-biological warfare and other fields.
Description
Technical field
The present invention relates to a kind of enrichment facility and application thereof, specifically a kind of with the device of magnetic Nano microsphere as the concentrated detection mark of carrier.Belong to the immunochromatographiassays assays technical field.
Background technology
Magnetic Nano microsphere has had very widely aspect biological and medical to be used, for example purifying, cell separation and pharmaceutical carrier of fixing, the immunoassay of protein and enzyme, RNA and DNA or the like.Utilize magnetic Nano microsphere as a token of the carrier of thing carry out immunochromatographiassays assays have the precision height, simple to operate, can be quantitative etc. advantage.Utilize the immunochromatographiassays assays of magnetic Nano microsphere will be more and more widely.
Is that connect on the surface of magnetic Nano microsphere can be to the antibody or the other biological active substance of specific cells identification based on magnetic Nano microsphere as the carrier that detects mark, so magnetic Nano microsphere can combine with the blip thing.The magnetic Nano microsphere enrichment that utilizes the magnetic action of magnet will be combined with mark concentrates, and carries out immunochromatography again, thereby has reached the purpose that can detect denier mark in the testing sample.For example, the enrichment of denier mark concentrates in blood, the urine sample; The enrichment of micro-nuisance concentrates in the milk; The enrichment of nuisance concentrates in the water source, or the like.
Therefore, design a kind of enrichment facility that can concentrate magnetic nano marker, can reduce the minimum detectability amount of routine immunization chromatography greatly, remedy the vacancy in market, make things convenient for the research needs of scientific research industries such as medical science, environment measuring.
Summary of the invention
Technical matters to be solved by this invention provides a kind of enrichment facility that can concentrate biological or other marks of denier in the test sample, thereby the sensing range of expansion routine immunization chromatography makes things convenient for the research needs of scientific research industries such as medical science, environment measuring.
Technical scheme of the present invention:
A kind of magnetic nano marker concentrating apparatus, comprise 1, be used to add the application of sample jar of testing sample, 5, drive the liquid pump of sample to be checked, the concentration systems of mark: 3, be used to carry the collection tube that concentrates the back mark, 4, be centered around high strength magnet around the collection tube, and 7, be connected between liquid pump and the collection tube can armoured magnetic field metal tube; And 2, the recycling can of recovery sample, 6, connect the conduit of application of sample jar and liquid pump, 8, the conduit of connection collection tube and recycling can.
A kind of improved magnetic nano marker concentrating apparatus, described application of sample jar 1 links to each other with the liquid pump at bottom opening and with conduit 6, can be so that testing sample can be fully by liquid pump water conservancy diversion.The internal diameter of the diameter of described metal catheter 7 and collection tube 3 is close, thereby makes sample liquid strengthen with the contact area of collection tube inwall in the collection tube of flowing through, so that the magnetic microsphere in the sample liquid can more effectively be adsorbed by magnetic field.Described collection tube 3 is transformed into the screwed test tube of the mouth of pipe, can connect supporting with it vinyl cover 9, and the junction has O-ring seal 10.Described vinyl cover 9 links to each other with high strength magnet 4, and is fixed in the square plastic box 11 with other each parts.During device work, analyte sample fluid by the conduit 6 liquid pump 5 of flowing through, arrives collection tube 3 from application of sample jar 1, flow to recycling can 2 at last.After concentrated finishing, collection tube 3 can easily be taken off the operation that experimentizes.
Described container: application of sample jar, collection tube and recycling can are selected right cylinder, rectangular parallelepiped or other shapes for use.
The application of described magnetic nano marker concentrating apparatus, analyte sample fluid is poured in the application of sample jar, and add a certain amount of immune magnetic microsphere, open the liquid pump after the mixed number minute, when analyte sample fluid is flowed through concentration systems, high-intensity magnetic field on every side promptly can be adsorbed on the collection tube inwall firmly with magnetic microsphere, after analyte sample fluid all flows into recycling can from the application of sample jar, concentrate and finish, take off collection tube this moment, adds the immunochromatographiassays assays that just can carry out mark after specific damping fluid mixes again.
Described mark is: albumen, gene, enzyme, virus, cancer embryo, or hormone.
Apparatus of the present invention are widely used in biology, medical science, research, food, environment, quarantine, anti-terrorism anti-biological warfare field.
Beneficial effect of the present invention: by above disclosed technical scheme as can be known, simple in structure, easy to operate, thickening efficiency advantages of higher that the present invention has.
Description of drawings
Fig. 1 structural representation of the present invention; 1, application of sample jar, 5, the liquid pump, 3, collection tube, 4, high strength magnet, 7, metal tube that can armoured magnetic field, 2, recycling can, 6, connect the conduit of application of sample jar and liquid pump, 8, the conduit of connection collection tube and recycling can.
Fig. 2 specific embodiment structural representation of the present invention.1, application of sample jar, 5, the liquid pump, 3, collection tube, 4, high strength magnet, 7, metal tube that can armoured magnetic field, 2, recycling can, 6, connect the conduit of application of sample jar and liquid pump, 8, the conduit of connection collection tube and recycling can, 9, plastics
, 10, O-ring seal, 11, the square plastic box.
Embodiment
Further specify the present invention below in conjunction with accompanying drawing.
Embodiment 1: referring to Fig. 1, this magnetic nano marker concentrating apparatus, comprise the application of sample jar 1 that is used to add testing sample, the recycling can 2 of recovery sample is used to carry the collection tube 3 that concentrates the back mark, is centered around collection tube high strength magnet 4 on every side, and the liquid pump 5 that is used to drive sample to be checked, described each parts link to each other with 8 by conduit 6, and the metal catheter 7 with the energy armoured magnetic field between liquid pump and the collection tube links to each other, and to avoid strong magnetic action magnetic microsphere is adsorbed on pipe inner wall.
Described container: application of sample jar, collection tube and recycling can be right cylinder, rectangular parallelepiped and other shapes.
Embodiment 2: as a further improvement on the present invention, develop on the basis of Fig. 1 and easier, the marker concentrating apparatus of hommization more of operation.As shown in Figure 2, described application of sample jar 1 links to each other with liquid pump 5 at bottom opening and with conduit 6, can be so that testing sample can be fully by liquid pump water conservancy diversion.The internal diameter of the diameter of described metal catheter 7 and collection tube 3 is close, thereby makes sample liquid strengthen with the contact area of collection tube inwall in the collection tube of flowing through, so that the magnetic microsphere in the sample liquid can more effectively be adsorbed by magnetic field.Described collection tube 3 is transformed into the screwed test tube of the mouth of pipe, can connect supporting with it vinyl cover 9, and the junction has O-ring seal 10.Described vinyl cover 9 links to each other with high strength magnet 4, and is fixed in the square plastic box 11 with other each parts.During device work, analyte sample fluid by the conduit 6 liquid pump 5 of flowing through, arrives collection tube 3 from application of sample jar 1, flow to recycling can 2 at last.After concentrated finishing, collection tube 3 can easily be taken off the operation that experimentizes.
The above only is preferred embodiment of the present invention, is not to be used for limiting the scope of the present invention.All equalizations of doing according to the scope of the invention change and modify, and are the present invention and contain.
Claims (6)
1, a kind of magnetic nano marker concentrating apparatus, it is characterized in that comprising the application of sample jar (1) that is used to add testing sample, drive the liquid pump (5) of testing sample, the concentration systems of mark: be used to carry the collection tube (3) that concentrates the back mark, be centered around the high strength magnet (4) around the collection tube, and be connected between liquid pump and the collection tube can armoured magnetic field metal tube (7); And the recycling can of recovery sample (2), the conduit (6) of connection application of sample jar and liquid pump, the conduit (8) of connection collection tube and recycling can.
2, device according to claim 1 is characterized in that connecting the application of sample jar and is connected with the liquid pump by application of sample pot bottom opening with the conduit (6) of liquid pump; The straight warp of metal tube (7) is close with the internal diameter of collection tube (3); Collection tube (3) is transformed into the screwed test tube of the mouth of pipe, connects supporting with it vinyl cover (9), and the junction has O-ring seal (10).
3, device according to claim 1 and 2 is characterized in that described container: application of sample jar, collection tube and recycling can are selected right cylinder, rectangular parallelepiped or other shapes for use.
4, the application of the described magnetic nano marker concentrating apparatus of claim 1, it is characterized in that analyte sample fluid is poured in the application of sample jar, and add a certain amount of immune magnetic microsphere, open the liquid pump after the mixed number minute, when analyte sample fluid is flowed through concentration systems, high-intensity magnetic field on every side promptly can be adsorbed on the collection tube inwall firmly with magnetic microsphere, after analyte sample fluid all flows into recycling can from the application of sample jar, concentrate and finish, take off collection tube this moment, adds the immunochromatographiassays assays that just can carry out mark after specific damping fluid mixes again.
5, application according to claim 4 is characterized in that described mark is: albumen, gene, enzyme, virus, cancer embryo, or hormone.
6, application according to claim 4 is characterized in that being widely used in biology, medical science, research, food, environment, quarantine, reaches anti-terrorism anti-biological warfare field.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008102446844A CN101435754A (en) | 2008-11-24 | 2008-11-24 | Magnetic nano marker concentrating apparatus and uses thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008102446844A CN101435754A (en) | 2008-11-24 | 2008-11-24 | Magnetic nano marker concentrating apparatus and uses thereof |
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| Publication Number | Publication Date |
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| CN101435754A true CN101435754A (en) | 2009-05-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2008102446844A Pending CN101435754A (en) | 2008-11-24 | 2008-11-24 | Magnetic nano marker concentrating apparatus and uses thereof |
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| CN (1) | CN101435754A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101539251B (en) * | 2009-04-24 | 2011-01-19 | 周华 | LED illuminating system with high power factor |
| CN103454135B (en) * | 2012-12-20 | 2015-07-01 | 北京莱伯泰科仪器股份有限公司 | Device for combining sample enrichment with analytical instrument |
-
2008
- 2008-11-24 CN CNA2008102446844A patent/CN101435754A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101539251B (en) * | 2009-04-24 | 2011-01-19 | 周华 | LED illuminating system with high power factor |
| CN103454135B (en) * | 2012-12-20 | 2015-07-01 | 北京莱伯泰科仪器股份有限公司 | Device for combining sample enrichment with analytical instrument |
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Open date: 20090520 |