CN101432313B - 极长链菊粉 - Google Patents
极长链菊粉 Download PDFInfo
- Publication number
- CN101432313B CN101432313B CN2007800151391A CN200780015139A CN101432313B CN 101432313 B CN101432313 B CN 101432313B CN 2007800151391 A CN2007800151391 A CN 2007800151391A CN 200780015139 A CN200780015139 A CN 200780015139A CN 101432313 B CN101432313 B CN 101432313B
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- Prior art keywords
- inulin
- food
- mixture
- extract
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 title claims abstract description 344
- 229920001202 Inulin Polymers 0.000 title claims abstract description 338
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- 238000000034 method Methods 0.000 claims description 106
- 235000013305 food Nutrition 0.000 claims description 85
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 85
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- 239000005715 Fructose Substances 0.000 claims description 51
- 239000000243 solution Substances 0.000 claims description 50
- 229930091371 Fructose Natural products 0.000 claims description 49
- 239000000047 product Substances 0.000 claims description 49
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 48
- 238000001556 precipitation Methods 0.000 claims description 43
- 239000000463 material Substances 0.000 claims description 42
- 239000008103 glucose Substances 0.000 claims description 37
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 33
- 238000004458 analytical method Methods 0.000 claims description 30
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- 229930006000 Sucrose Natural products 0.000 claims description 21
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 21
- 239000005720 sucrose Substances 0.000 claims description 21
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 17
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- 238000000149 argon plasma sintering Methods 0.000 claims description 9
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- 238000010298 pulverizing process Methods 0.000 claims description 9
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 8
- 229910001425 magnesium ion Inorganic materials 0.000 claims description 8
- 239000011575 calcium Substances 0.000 claims description 7
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 235000011950 custard Nutrition 0.000 claims description 5
- 239000000835 fiber Substances 0.000 claims description 5
- 230000000087 stabilizing effect Effects 0.000 claims description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
- 235000015895 biscuits Nutrition 0.000 claims description 4
- 210000000481 breast Anatomy 0.000 claims description 4
- 235000014121 butter Nutrition 0.000 claims description 4
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 4
- 239000000920 calcium hydroxide Substances 0.000 claims description 4
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 4
- 235000015872 dietary supplement Nutrition 0.000 claims description 4
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical compound [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 claims description 4
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- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 claims description 3
- 239000011654 magnesium acetate Substances 0.000 claims description 3
- 235000011285 magnesium acetate Nutrition 0.000 claims description 3
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- UVNKIYPMUDFRGV-UHFFFAOYSA-N [Mg].C(CC)(=O)O Chemical compound [Mg].C(CC)(=O)O UVNKIYPMUDFRGV-UHFFFAOYSA-N 0.000 claims description 2
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0051—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Fructofuranans, e.g. beta-2,6-D-fructofuranan, i.e. levan; Derivatives thereof
- C08B37/0054—Inulin, i.e. beta-2,1-D-fructofuranan; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
材料 | Mw[g/mol] | Mn[g/mol] | 聚合物分布(最小-最大)[g/mol] | DPw | DPn | 分子分散度 |
朝鲜蓟菊粉 | 13995 | 11620 | 1377-33099 | 86 | 72 | 1.19 |
Raftiline HP | 5823 | 4759 | 99915162 | 36 | 29 | 1.24 |
大丽花菊粉 | 6678 | 5358 | 1139-19569 | 41 | 33 | 1.24 |
材料 | 葡萄糖(g/100g菊粉粉末) | 果糖(g/100g菊粉粉末) | 蔗糖(g/100g菊粉粉末) |
朝鲜蓟菊粉 | <0.1% | <0.1% | <0.1% |
样品 | 2-1,6-果糖[mol%]* |
菊粉 朝鲜蓟Raftiline HP大丽花洋姜 | 1.40.40.2未检测到 |
材料 | To[℃] | 熔化焓dH[J/g] |
朝鲜蓟菊粉 | 41.1 | 25.2 |
Raftiline HP | 37.8 | 22.8 |
筛格宽度/μm | 质量/g | % |
<63 | 14.00 | 28.97 |
<90 | 6.35 | 13.14 |
<125 | 7.54 | 15.60 |
<160 | 5.53 | 11.44 |
<200 | 4.75 | 9.83 |
<500 | 10.05 | 20.79 |
>500 | 0.11 | 0.23 |
总计 | 48.33 | 100.00 |
筛格宽度/μm | 质量/g | % |
<63 | 8.33 | 16.74 |
<90 | 3.92 | 7.88 |
<125 | 6.17 | 12.40 |
<160 | 6.05 | 12.16 |
<200 | 7.60 | 15.28 |
<500 | 17.62 | 35.42 |
>500 | 0.06 | 0.12 |
总计 | 49.75 | 100.00 |
结晶度xc[%] | 无序度k[10-2nm2] | D(hkl)2θ=8°[nm] | D(hkl)2θ=12°[nm] | |
测试1 | 非结晶 | -- | -- | -- |
测试2 | 非结晶 | -- | -- | -- |
测试3 | 5-10 | -- | -- | -- |
测试4 | 38 | 2.9 | 7.0 | 9.6 |
测试5 | 45 | 2.9 | 6.9 | 9.4 |
测试6 | 33 | 4.4 | 5.5 | 7.1 |
测试7 | 非结晶 | -- | -- | -- |
测试8 | 15 | 3.2 | 8.1 | 9.9 |
探针 | 目标种属 | 序列(5’至3’) | 杂交温度/℃ |
Bif164 | 双歧杆菌属菌种 | CATCCGGCATTACCACCC | 50 |
Bac303 | 类杆菌属菌种 | CCAATGTGGGGGACCTT | 45 |
Chis150 | 溶组织梭菌群 | TTTCCYTCTAATTATGGCGTATT | 50 |
Lab158 | 乳酸杆菌属/肠球菌属菌种 | GGTATTAGCATCTGTTTCCA | 50 |
Ato291 | 奇异菌属菌群 | GGTCGGTCTCTCAACCC | 50 |
Erec482 | 球形梭菌-直肠真杆菌群 | GCTTCTTAGTCARGTACCG | 52 |
菊粉含量 | Raftiline | 占对照的% | 菊粉DPw=86 | 占对照的% |
0 | 2.06 | 2.09 | ||
5%5分钟 | 2.08 | 101 | 2.18 | 105 |
10%5分钟 | 2.09 | 101 | 2.38 | 114 |
0 | 2.7 | 2.68 | ||
5%10分钟 | 2.73 | 101 | 2.9 | 108 |
10%10分钟 | 2.93 | 109 | 3.09 | 115 |
0 | 3.25 | 3.06 | ||
5%15分钟 | 3.28 | 101 | 3.42 | 112 |
10%15分钟 | 3.38 | 104 | 3.70 | 121 |
Claims (30)
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP06090064 | 2006-04-28 | ||
EP06090064.4 | 2006-04-28 | ||
US79681906P | 2006-05-02 | 2006-05-02 | |
US60/796,819 | 2006-05-02 | ||
EP06090200.4 | 2006-10-27 | ||
EP06090200 | 2006-10-27 | ||
US85525006P | 2006-10-30 | 2006-10-30 | |
US60/855,250 | 2006-10-30 | ||
PCT/EP2007/004029 WO2007128560A2 (en) | 2006-04-28 | 2007-04-27 | Inulin of very high chain length |
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CN101432313A CN101432313A (zh) | 2009-05-13 |
CN101432313B true CN101432313B (zh) | 2013-08-07 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999024593A1 (en) * | 1997-11-06 | 1999-05-20 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Nucleic acid molecules which encode proteins having fructosyl transferase activity and methods for producing long-chain inulin |
CN1422547A (zh) * | 2001-12-06 | 2003-06-11 | 大连绿山科技有限公司 | 菊粉的加工方法 |
CN1610750A (zh) * | 2001-09-26 | 2005-04-27 | 富士日本精糖株式会社 | 制备菊粉的方法 |
CN1634997A (zh) * | 2004-12-02 | 2005-07-06 | 西北农林科技大学 | 一种菊粉的提取工艺 |
-
2007
- 2007-04-27 ES ES07724953T patent/ES2356690T3/es active Active
- 2007-04-27 CN CN2007800151391A patent/CN101432313B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999024593A1 (en) * | 1997-11-06 | 1999-05-20 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Nucleic acid molecules which encode proteins having fructosyl transferase activity and methods for producing long-chain inulin |
CN1610750A (zh) * | 2001-09-26 | 2005-04-27 | 富士日本精糖株式会社 | 制备菊粉的方法 |
CN1422547A (zh) * | 2001-12-06 | 2003-06-11 | 大连绿山科技有限公司 | 菊粉的加工方法 |
CN1634997A (zh) * | 2004-12-02 | 2005-07-06 | 西北农林科技大学 | 一种菊粉的提取工艺 |
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