CN101430302A - Production method of nitric oxide sensor - Google Patents
Production method of nitric oxide sensor Download PDFInfo
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- CN101430302A CN101430302A CNA2008102369462A CN200810236946A CN101430302A CN 101430302 A CN101430302 A CN 101430302A CN A2008102369462 A CNA2008102369462 A CN A2008102369462A CN 200810236946 A CN200810236946 A CN 200810236946A CN 101430302 A CN101430302 A CN 101430302A
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- carbon paste
- nitric oxide
- paste electrode
- trimethyl ammonium
- ammonium bromide
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Abstract
The invention discloses a method for preparing a nitric oxide sensor, which comprises the following steps: a pasty mixture of graphite powder and paraffin oil is squeezed into a polytetraethene tube or a glass tube; a copper wire is led out to prepare a carbon paste electrode; a cetyltrimethylammonium bromide membrane electrode is obtained by immersing the polished carbon paste electrode fully in cetyltrimethylammonium bromide solution, taking the carbon paste electrode out for washing in water and naturally drying the carbon paste electrode; finally, Nafion solution is dropped on the surface of the cetyltrimethylammonium bromide membrane electrode to obtain the nitric oxide sensor with the carbon paste electrode modified by the cetyltrimethylammonium bromide and the Nafion. The produced sensor has the advantages that the cost is low, the preparation process is simple, technological parameters are easy to control and the biomedical application prospect is wide.
Description
Technical field
The present invention relates to a kind of preparation method of nitric oxide sensor, belong to electrochemical field.
Background technology
Nitrogen monoxide is the important biological messenger molecule, and the physiology course of biosome is played important regulatory role, and the vasodilation messenger molecule can prevent platelet aggregation and have immunologic function etc. in this way.Lack NO and may cause diabetes, angiocardiopathy and other diseases.Nitrogen monoxide is very active and unstable, the half life period short (5-60s), so it measures very difficulty.At present, being usually used in detecting nitric oxide production method has chemoluminescence method, ultravioletvisible spectroscopy, fluorescent spectrometry, ESR spectrum method, electrochemical method etc.In all methods, electrochemical method has simple to operate, and is highly sensitive, and selectivity is good, can realize advantage real-time, in situ detection again, and be widely used in the biomedicine, and become nitric oxide production important research instrument in the modern biomedical.Common electrode has glass-carbon electrode, gold electrode and platinum electrode, and metal porphyrins, metallo phthalocyanine, schiff base metal complex are generally arranged electrode modified material and other is as o-phenylenediamine family macromolecule material and nano material etc.But these material price general charged costlinesses have greatly limited large-scale practical application.
Summary of the invention
The object of the invention just provides a kind of preparation method of nitric oxide sensor, produce a kind of surfactant that utilizes and detect nitric oxide production electrochemical sensor in the hydrophobic absorption on carbon paste electrode surface, the nitric oxide sensor of this method preparation not only has the characteristics of good stability, and this method used with low cost, technology simple, be convenient to large-scale industrial application.
Realize that the technical scheme that the object of the invention adopts is: the preparation method of nitric oxide sensor may further comprise the steps: with dag and paraffin oil mixing furnishing pasty state, be squeezed into then in poly-four ethylene tubes or the glass tube, and draw copper cash and be made into carbon paste electrode, the carbon paste electrode of preparation is polished smooth, in hexadecyl trimethyl ammonium bromide solution, soak fully, take out then in water, to swing and wash, the nature airing is the hexadecyl trimethyl ammonium bromide membrane electrode, get Zeo-karb Nafion solution at last and drip to hexadecyl trimethyl ammonium bromide membrane electrode surface, make the nitric oxide sensor of hexadecyl trimethyl ammonium bromide and Nafion decorating carbon paste electrode.
Above-mentioned carbon paste electrode with preparation polishes smooth to be placed in the hexadecyl trimethyl ammonium bromide solution on pan paper and soaks.
Above-mentioned dag is mixed the furnishing pasty state with paraffin oil with the ratio of 100/16 milligram/microlitre, be squeezed into then in poly-four ethylene tubes or the glass tube, and draw copper cash and be made into carbon paste electrode, the carbon paste electrode of preparation is polished smooth, in 0.01mol/L hexadecyl trimethyl ammonium bromide solution, soaked 20 seconds, take out then in water, to swing and wash 30 seconds, the nature airing is the hexadecyl trimethyl ammonium bromide membrane electrode, get 2 microlitre 0.1%Nafion solution at last and drip to the surface, make the nitric oxide sensor of hexadecyl trimethyl ammonium bromide and Nafion decorating carbon paste electrode.
The present invention is based on the hydrophobic absorption of surfactant on the carbon paste electrode surface and prepares electrochemical sensor, drips then to be coated with antijamming capability and the stability that Nafion improves nitric oxide sensor.The current-responsive of the nitric oxide sensor that makes and NO concentration are 3.6 * 10
-8～1.8 * 10
-5Present good linear relationship in the mol/L scope, related coefficient is 0.9994, detects and is limited to 1.8 * 10
-8Mol/L.This sensor is used for the measurement of mouse lung haemocyte NO burst size under medicine irritation and inhibition, and the result is satisfactory.
The outstanding feature of the nitrogen monoxide microsensor of the present invention's preparation is:
1. the stability of this sensor and favorable reproducibility, the range of linearity is wide, and for other conventional nitric oxide sensor, the detectability of this sensor is low.
2. this sensor is highly sensitive, and the response time can be low to moderate 3 seconds.
3. for numerous synthetic expensive reagent, surfactant has advantages such as low price, stability and Bc are good.
4. the preparation technology that should invent is simple, can realize suitability for industrialized production, and is easy to operate when detecting nitrogen monoxide, is applicable to practical applications such as biologic medical.
Description of drawings
The present invention is further illustrated below in conjunction with the drawings and specific embodiments.
Fig. 1 is containing NO phosphate buffer (PBS, 0.1mol/L, pH=7.4) cyclic voltammogram in for the different modifying electrode.
Fig. 2 is that the nitric oxide sensor of Nafion/CTAB/CPE is adding the ampere response of variable concentrations NO continuously.
Fig. 3 is the steady-state response electric current of NO among Fig. 2 and the graph of relation between the concentration.
Fig. 4 is the nitrogen monoxide dispose procedure in the nitric oxide sensor monitoring mouse lung tissue of the present invention's preparation.
Embodiment
The spectroscopically pure graphite powder is mixed the furnishing pasty state with paraffin oil with the ratio of 100 milligrams/16 microlitres, be squeezed in homemade poly-four ethylene tubes, and draw conduction with copper cash.This electrode is polished smooth on pan paper, soaked 20 seconds in 0.01mol/L CTAB solution, take out to swing in water and wash 30 seconds, natural airing is CTAB/CPE.Get 2 microlitres, 0.1% Nafion solution with micro syringe and drip to CTAB membrane electrode surface, preparation Nafion/CTAB/CPE nitric oxide sensor.
In cyclic voltammogram shown in Figure 1, curve c shows that NO goes up the non-oxidation peak at naked carbon paste electrode (CPE).Curve a shows that with behind the CTAB modification CPE high oxidation peak occurs at the 0.60V place, peak shape is sharp-pointed, and peak current reaches 56 microamperes.Curve b be shown as further add be coated with the Nafion film after, peak current descends 30%, but the negative 20mV that moves of spike potential has a weak oxide peak at the 0.73V place simultaneously, this peak is the nitrogen monoxide diffuse peak.If electrode is transferred to first lap scanning in the blank phosphoric acid buffer, peak I keeps, and peak II disappears.Find relatively that by these CTAB film presents good electro catalytic activity and sensitization to the oxidation of NO.
In ampere response diagram shown in Figure 2, the nitric oxide sensor adding NO concentration that curve a, b, c are respectively the Nafion/CTAB/CPE modification is 3.6 * 10
-8Mol/L mol/L, 1.8 * 10
-7Mol/L, 1.8 * 10
-6The ampere response of mol/L.As seen from the figure, add not the concentration nitrogen monoxide after, the sensor current response all increased sharply and reach stable state in the time faster.The steady-state response electric current of NO and the relation curve between the concentration as shown in Figure 3, the result shows that the current-responsive of sensor and NO concentration are 3.6 * 10
-8～1.8 * 10
-5Present good linear relationship in the mol/L scope, I (receive peace)=2.2476+84.3607 * C
NO(μ mol/L), related coefficient is 0.9994; Detection is limited to 1.8 * 10
-8Mol/L.
Fig. 4 prepares the dispose procedure of NO in the NO sensor monitors mouse lung tissue for using the present invention.When adding the precursor L-arginine (L-Arg) of 1.0mmol/L NO in the PBS solution, slowly (curve a) in the sensor current response.When the response time reached 200 seconds in the sensor insertion lung tissue sample (20mg lungs tissue/1.0mL PBS), to the precursor L-Arg that wherein adds 1.0mmol/LNO, it was obvious to observe current-responsive, and after-current response in about 80 seconds reaches a steady-state value (curve b).Show that nitricoxide synthase in the lung tissue (NOS) can discharge NO continuously under the stimulation of L-Arg, can estimate the concentration that discharges NO in the lung tissue according to the Sensitirity va1ue of the sensor that obtains previously is 1.4 μ mol/L.
Claims (4)
1. the preparation method of a nitric oxide sensor, it is characterized in that may further comprise the steps: carbon paste electrode is polished smooth, in hexadecyl trimethyl ammonium bromide solution, soak fully, take out then in water, to swing and wash, the nature airing is the hexadecyl trimethyl ammonium bromide membrane electrode, get Nafion solution at last and drip to hexadecyl trimethyl ammonium bromide membrane electrode surface, make the nitric oxide sensor of hexadecyl trimethyl ammonium bromide and Nafion decorating carbon paste electrode.
2. according to the preparation method of the described nitric oxide sensor of claim 1, it is characterized in that: the carbon paste electrode for preparing is polished smooth to be placed in the hexadecyl trimethyl ammonium bromide solution on pan paper soak.
3. according to the preparation method of claim 1 or 2 described nitric oxide sensors, it is characterized in that: carbon paste electrode is with dag and paraffin oil mixing furnishing pasty state, is squeezed into then in poly-four ethylene tubes or the glass tube, and draws copper cash and make.
4. according to the preparation method of the described nitric oxide sensor of claim 3, it is characterized in that: dag is mixed the furnishing pasty state with paraffin oil with the ratio of 100/16 milligram/microlitre, be squeezed into then in poly-four ethylene tubes or the glass tube, and draw copper cash and be made into carbon paste electrode, the carbon paste electrode of preparation is polished smooth, in 0.01mol/L hexadecyl trimethyl ammonium bromide solution, soaked 20 seconds, take out then in water, to swing and wash 30 seconds, the nature airing is the hexadecyl trimethyl ammonium bromide membrane electrode, get 2 microlitres, 0.1% Nafion solution at last and drip to the surface, make the nitric oxide sensor of hexadecyl trimethyl ammonium bromide and Nafion decorating carbon paste electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008102369462A CN101430302A (en) | 2008-12-19 | 2008-12-19 | Production method of nitric oxide sensor |
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|---|---|---|---|
| CNA2008102369462A CN101430302A (en) | 2008-12-19 | 2008-12-19 | Production method of nitric oxide sensor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103531306A (en) * | 2013-10-18 | 2014-01-22 | 武汉大学 | Graphite conducting film as well as preparation method and application of graphite conducting film |
| CN106415255A (en) * | 2014-03-02 | 2017-02-15 | 麻省理工学院 | Gas sensors based upon metal carbon complexes |
| CN107367536A (en) * | 2017-08-31 | 2017-11-21 | 常州市丰瑞电子有限公司 | A kind of preparation method of stable type carbon paste electrode |
-
2008
- 2008-12-19 CN CNA2008102369462A patent/CN101430302A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103531306A (en) * | 2013-10-18 | 2014-01-22 | 武汉大学 | Graphite conducting film as well as preparation method and application of graphite conducting film |
| CN103531306B (en) * | 2013-10-18 | 2015-08-19 | 武汉大学 | A kind of graphite film and preparation method thereof and application |
| CN106415255A (en) * | 2014-03-02 | 2017-02-15 | 麻省理工学院 | Gas sensors based upon metal carbon complexes |
| CN107367536A (en) * | 2017-08-31 | 2017-11-21 | 常州市丰瑞电子有限公司 | A kind of preparation method of stable type carbon paste electrode |
| CN107367536B (en) * | 2017-08-31 | 2019-10-29 | 东阳市天齐知识产权运营有限公司 | A kind of preparation method of stable type carbon paste electrode |
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Application publication date: 20090513 |