CN101427736B - Method for preparing peanut concentrated protein with alcohol method - Google Patents

Abstract

A preparation method of concentrated peanut protein by using alcoholic method, belonging to the technology field of comprehensive utilization of peanut meal, comprises pulverizing high-temperature peanut meal, performing enzymolysis under an acid environment, deactivating enzyme, washing with alcohol, centrifuging, washing sediment, and freeze drying to obtain concentrated peanut protein with better functions and properties. The obtained product has the advantages of easily accessible raw materials, low cost, simple method, no toxicity, good safety and high yield; and can be widely used in feedstuff for young animals and pets with high production value. The inventive method is reasonably designed, and is important for realizing comprehensive utilization of peanut meal and improving the added value thereof.

Description

A kind of preparation method of peanut concentrated protein with alcohol method

Technical field

A kind of preparation method of peanut concentrated protein with alcohol method belongs to peanut meal comprehensive utilization technique field.

Background technology

Peanut is the annual herb plant of pulse family Arachis.Peanut is one of the whole world most important four big oil crops, the peanut cultivation area cumulative year after year of China, and output has surpassed 1,500 ten thousand tons, occupies the first in the world.Except that directly edible, be mainly used in oil expression at China's peanut, annually after oil expression, can obtain surpassing 9,000,000 tons peanut meal,, further do not develop and utilize the general direct feed of the peanut meal after the oil expression as fish and fowl poultry.Protein content reaches 40%～50% in the peanut meal, and the nutritive value of peanut protein is higher.And China is not enough to the utilization of peanut meal at present, and wherein the production aspect research of protein is not in place, and recovery rate is lower, and quality does not reach the food-grade requirement.Therefore the production technology of the peanut concentrated protein with alcohol method further approach of development and use peanut protein of can yet be regarded as is the developing direction of peanut meal converted products.At home and abroad there is no enzymolysis and add the report that alcohol is washed peanut meal.

Summary of the invention

The preparation method who the purpose of this invention is to provide peanut concentrated protein with alcohol method is used to develop the feed of high added value.

Technical scheme of the present invention: a kind of preparation method of peanut concentrated protein with alcohol method is a raw material with the high temperature peanut meal, through pulverize, enzymolysis, the enzyme that goes out, alcohol are washed under the sour environment, centrifugal, washing precipitation is after freeze drying obtains the product peanut concentrated protein; Step is:

(1) raw material pulverizing: pulverize peanut meal, cross 50 eye mesh screens;

(2) enzymolysis under the sour environment: press 1: 8 part by weight of peanut meal powder and water and add water, regulator solution pH value is to 4.5-4.7, and temperature 45-50 ℃, in the ratio interpolation acid protease of [E/S]=0.1%-0.15%, institute adds acid protease enzyme 3000u/g alive; Ratio in [E/S]=3%-4% is added carbohydrase, and institute adds glucoamylase enzyme 100000u/g alive; Keep pH value, temperature-resistant, constant temperature vibration hydrolysis 1.5～2.5h;

(3) enzyme that goes out: be warming up to 80 ℃ of enzyme 10min that go out, cooling fast behind the enzyme that goes out;

(4) alcohol is washed: directly add absolute ethyl alcohol and transfer to concentration of alcohol to 70% in (3) gained reaction feed liquid, 50 ℃ of pure down washing 2h;

(5) centrifugation: (4) gained reaction feed liquid is carried out centrifugation 3000r/min, 20min;

(6) washing precipitation: adopt 70% ethanol washing (5) gained precipitation, wash 2-3 time;

(7) freeze drying: freeze drying (6) gained precipitation, freeze drying vacuum is 10～30Pa, freeze temperature is lower than-30 ℃, drying time 20～24h, moisture is controlled at below 5%, dried product is peanut concentrated protein.

Beneficial effect of the present invention: the raw materials used wide material sources of the present invention, with low cost, method is easy, non-toxic and safe, product yield height, products obtained therefrom can be widely used in the young animal feed and feed for pet than Peak output.The inventive method has simply, and is efficient, the output height, and product function character is superiority preferably.The technology of the present invention design science is reasonable, to the comprehensive utilization that realizes high temperature peanut meal with improve its added value and have great importance.

Description of drawings

Fig. 1 technological process of production figure.

The specific embodiment

Embodiment 1

Take by weighing through pulverizing, cross the 100g peanut meal powder of 50 mesh sieves, add entry 800mL stirring and evenly mixing, regulate pH value to 4.5, add 0.1g acid protease (enzyme 3000u/g alive), add 5mL carbohydrase (enzyme 100000u/g alive) again, behind 50 ℃ of following constant temperature vibration 2h, temperature is risen to 80 ℃, enzyme 10min goes out, directly add absolute ethyl alcohol 2400mL, regulation system concentration of alcohol to 70%, 50 ℃ of following alcohol are washed 2h, 3000r/min centrifugation 20min, the gained precipitation adds aqueous dispersion after washing 2-3 time with 70% ethanol, and adjust pH is 7.0, freeze drying promptly gets the product peanut concentrated protein.

Claims (1)

1. the preparation method of a peanut concentrated protein with alcohol method is characterized in that with the high temperature peanut meal being raw material, through pulverize, enzymolysis, the enzyme that goes out, alcohol are washed under the sour environment, centrifugal, washing precipitation is after freeze drying obtains the product peanut concentrated protein; Step is:

(1) raw material pulverizing: pulverize peanut meal, cross 50 eye mesh screens;

(2) enzymolysis under the sour environment: press 1: 8 part by weight of peanut meal powder and water and add water, regulator solution pH value is to 4.5-4.7, and temperature 45-50 ℃, in the ratio interpolation acid protease of [E/S]=0.1%-0.15%, institute adds acid protease enzyme 3000U/g alive; Ratio in [E/S]=3%-4% is added carbohydrase, and institute adds glucoamylase enzyme 100000U/g alive; Keep pH value, temperature-resistant, constant temperature vibration hydrolysis 1.5～2.5h;

(3) enzyme that goes out: be warming up to 80 ℃ of enzyme 10min that go out, cooling fast behind the enzyme that goes out;

(4) alcohol is washed: directly add absolute ethyl alcohol and transfer to concentration of alcohol to 70% in (3) gained reaction feed liquid, 50 ℃ of pure down washing 2h;

(5) centrifugation: (4) gained reaction feed liquid is carried out centrifugation 3000r/min, 20min;

(6) washing precipitation: adopt 70% ethanol washing (5) gained precipitation, wash 2-3 time;

(7) freeze drying: freeze drying (6) gained precipitation, freeze drying vacuum is 10～30Pa, freeze temperature is lower than-30 ℃, drying time 20～24h, moisture is controlled at below 5%, dried product is peanut concentrated protein with alcohol method.

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