CN101422633A

CN101422633A - Preparation method of no-skin wound skin-grafting slice

Info

Publication number: CN101422633A
Application number: CNA2008101476683A
Authority: CN
Inventors: 陈启忠; 李开贤; 赵娟; 陈建; 陈蓉; 陈兆涛; 王利平
Original assignee: Individual
Current assignee: Individual
Priority date: 2008-11-25
Filing date: 2008-11-25
Publication date: 2009-05-06
Also published as: WO2010060334A1

Abstract

The invention relates to a preparation method of a wound skinless grafting plate, and discloses a method for using a tail muscle of nutria that is a ganglion consisting of pure natural biological glue prion proteins to be expanded to a plate shape by a modern biological fermenting process. As the physical property of the tail muscle of nutria does not reject, is easy to be combined with tissue organizations and has no toxic side effects, the tail muscle, after protease enzymatic hydrolysis cramping and extending plate by physical treatments, is a natural material used as manmade skin, and is a perfect skin grafting material for existing clinical burning, scalding, skin wound caused by accidents and face-lifting, and the wound skinless grafting plate can combine the tissue to resist infections, maintain moisture, promote new granulation organizations of the tissue to grow fast and the wound to recover fast, exempt pains of patients when in drug-changing, especially plays an irreplaceable role in protecting the wound of clinical large-area deep burning and scalding from infections and promote the growing of the new granulation organizations, vascular and the like to protect the wound and promote recovery, therefore, the wound skinless grafting plate is a perfect substitute of human true skin.

Description

Preparation method of no-skin wound skin-grafting slice

One, technical field

The present invention relates to a kind of human body skin skin-grafting that is used for, substitute the preparation method of corium, coypu is a rodent, its two hind leg toe has web to live in water, about about 5-10 kilograms of body weight, its fur warmth retention property is good, it is the first-class raw material of making the fur coat clothing, its meat contains rich in protein and various trace elements is good meat product, its oils and fats is the medicinal raw material of the multiple disease of surgery, it also is the senior raw material of advanced skin care product, sturdy about 30 centimeters long of the tail of adult coypu to 40 centimeters, there is big or small thickness long in the tail, the short tail muscle that differs is about more than 50, constitutes main neural tendon with collagen protein, also is one of Main Ingredients and Appearance of coypu tail, this tail muscle pulling force is strong, every can be made into 2-4 centimeters wide veneer coated tablet more than 10 kilograms, the same film-making of four-leg muscle of coypu, this tail muscle and foot muscle do not reject, can combine closely with when injected organism tissue, thereby utilize bio-fermentation process exhibition sheet to make human body skin, alternative corium is used for the product of human body skin-grafting.

Two, background technology

Technical background of the present invention is that tail muscle and the foot muscle of coypu is widely used in clinical again as suture, be the accurate font size products of traditional Chinese medicines, it is the collagen protein stitching thread of pure natural, it is better than the current all kinds of stitching thread of surgery that use clinically, and it does not reject and does not remove line clinically, wound is than like product healing in 3 days in advance, preventing from scar.Thereby, utilize the tail muscle of coypu and the characteristics of foot muscle not to reject, pulling force is strong, soft, good air permeability after processing, can combine with when injected organism tissue, fuse, it be made sheet replace the skin-grafting of human body corium to be used for the skin-grafting application replacement corium of human body skin burn, scald and human body skin accidental injury.

Three, summary of the invention

The present invention produces product by " fermentation technology " bio protease enzyme solution, the peeling of peeling method routinely after coypu slaughtered, after peeling the tail skin tail is cut down from the root of the tail portion of coypu, with cutter tail is divided into pintongs, cut off four-leg more respectively and peel foot limb skin, tail and four-leg after the decortication are put into fermentation tank simultaneously, add simultaneously bio protease again, add bio protease and be tail and four-leg gross weight 0.1% to 10%, put into behind the fermentation tank tail handled well and four-leg airtight, heat-preservation fermentation, fermentation temperature is controlled between 20 ℃ to 60 ℃, constant temperature keeps after fermentation in 2 to 15 days to finish, take out tail and four-leg, from the tail bone and the four-leg bone extract tail muscle and foot muscle, putting into special tablet machine after taking off enzyme, to be pressed into veneer coated tablet long, wide the limit allows its natural drying formed stool be finished product.

Four, specific embodiments

Below in conjunction with example the present invention is described in further detail enforcement:

1, embodiment one

Coypu slaughtered cut off tail after the peeling and be fixed on the blocky flat board loosening, rapidly tail is on average cut into pintongs with sharp weapon, cut off four-leg more respectively, after handling well, tail and four-leg in time put into fermentation tank, and in time add bio protease, enzyme concentration is that 0.1% to 10% of zymolyte gross weight enters ferment tank, 60 ℃ to 45 ℃ in earlier stage of fermentation temperatures, along with the propelling of time progressively drops to 20 ℃, total fermentation, enzymolysis needs 2-15 days, variation fermentation temperature according to seasonal temperature can transfer to low early and high after 20 ℃-60 ℃, and fermented product stops fermenting, and back taking-up fermented product takes off enzyme extraction tail muscle and the compacting of foot muscle is finished product in flakes.

2, embodiment two

With bio protease, tail muscle and the foot muscle of enzymolysis maturation through extracting, to divide long and short, thick, thin different specifications classification to put into special tablet machine and be pressed into veneer coated tablet, width length is not limit, and is the finished product of different size through the natural drying formed stool.

The present invention has the following advantages: manufacture craft is simple, and the production process three-waste free discharge does not pollute Environment, product are that the biogum PrPC of pure natural consists of, and do not reject, to any injected organism tissue all Can in conjunction with, resistance infection is strong, Wound healing is fast, and is without any side effects to human body.

The surface of a wound need not hide the protection surface of a wound with medicinal gauze or other material after the skin-grafting, and it can the oneself be protected Protect the surface of a wound, it substitutes corium and is used for skin-grafting, and permeability is good, good water-retaining property. Permeability is strong, Pain when avoiding changing dressing for patient can play the protective effect of corium, is that present human body skin skin-grafting is excellent Good substitute.

Claims

1, claims denomination of invention is: the preparation method of no-skin wound skin-grafting slice, be to utilize the tail muscle of a kind of mammal coypu, the foot muscle of four-leg, the tail muscle of coypu, foot muscle are the neural tendons that collagen protein constitutes, it is the raw material of making the human body no-skin wound skin-grafting slice, the tail muscle of coypu and the foot muscle of extremity are produced through bio protease fermentation enzymolysis, extract tail and four-leg after coypu is slaughtered, after shedding, put in the fermentation tank; It is characterized in that adding bio protease, the amount that adds bio protease be tail and four-leg gross weight 0.1% to 10%, airtight heat-preservation fermentation, 20 ℃-60 ℃ of temperature constant temperature, 2-15 days heat-preservation fermentation time fermentation ends is taken out tail and four-leg, from the tail bone, extract tail muscle and foot muscle again with the four-leg bone, this muscle is pressed into veneer coated tablet length after taking off enzyme, width is not limit, and just becomes finished product.