CN101422485A - Preparation method of spleen byproducts for producing homology anti-serum blood and transfer factor from fox, raccoon dog, mink - Google Patents
Preparation method of spleen byproducts for producing homology anti-serum blood and transfer factor from fox, raccoon dog, mink Download PDFInfo
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- CN101422485A CN101422485A CNA2008100510935A CN200810051093A CN101422485A CN 101422485 A CN101422485 A CN 101422485A CN A2008100510935 A CNA2008100510935 A CN A2008100510935A CN 200810051093 A CN200810051093 A CN 200810051093A CN 101422485 A CN101422485 A CN 101422485A
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Abstract
The invention relates to a preparation method for obtaining blood used for preparing homologous antiserums and a spleen byproduct used for preparing transfer factors from the bodies of fur bearing animals such as foxes, raccoon dogs and minks; the furs of the foxes, the raccoon dogs and the minks are taken concentratedly in December and confirmed according to the fur-taking dates of different areas; healthy individuals are picked up 45 days before a predicated fur-taking date; the booster immunization of univalent vaccines or polyvalent vaccines of canine distemper, Canine parvovirus enteritis, adenovirus disease, corona virus laxness, parainfluenza, and pasteurellosis is carried out for 3 times by purified and condensed antigens on the foundation of carrying out immunization for once in summer; when the furs are taken, aseptic anticoagulation blood collection is carried out to a heart to improve the blood serum yield; and the spleens of the animals are collected for extracting specific or common transfer factors simultaneously. The blood can be conveniently prepared into the blood serums which resist communicable diseases, and the transfer factors can be extracted the spleen, thus providing guarantee for the healthy development of the breeding in the next year; the invention is used for curing the corresponding communicable diseases of the wild animals of the same species; and when a small amount of communicable disease cases appear in a breeding crowd, the corresponding pathogeny hyper-immune serums and transfer factors are mainly injected to a supposed healthy crowd after the pathogeny is confirmed so as to cure the affected animals in the early period of disease and control the spreading of the epidemic situations.
Description
Technical field:
The present invention relates to grade product preparation method that of a kind of Vulpes, Nyctereutes procyonoides, ermine homologous antiserum, transfer factor, be particularly related to a kind of obtaining simultaneously in fur-bearing animal Vulpes, Nyctereutes procyonoides, the ermine body and be used to the preparation method for preparing the blood of the sick serum of infection and be used to prepare the spleen of transfer factor, prepare the raw material of low-cost homologous antiserum and transfer factor.
Background technology
Infectiousness such as fur-bearing animal Vulpes, Nyctereutes procyonoides, ermine high-density breeding infectious disease pilosity, particularly canine distemper, parvovirus enteritis, adenopathy viral disease, coronavirus diarrhoea, parainfluenza, Bacillus pasteurii disease are strong, fatality rate is high, and are serious to the harm of fur-bearing animal aquaculture; The antiserum that virus, pathogenic bacteria cause the body generation is the specific drug of the corresponding disease of morbidity early treatment; The allogenic animal antiserum anaphylaxis is little, the therapeutic effect optimum, and morbidity can effectively be controlled epidemic situation in early days and spread.Yet fur-bearing animal emergency antiserum is based on heterogenous animal antiserum (as Canis familiaris L., sheep, donkey, rabbit etc.) all the time, and the heterogenous animal antiserum easily produces the anaphylaxis of serum allos composition, and prepares sero-fast heterozoic feeding cost height; It is few that present allogenic animal is used to prepare antiserum, and prior preparation method-only by getting healthy old beastly a small amount of venous blood emergence therapeutic, closely edge Canis familiaris L. Antiserum Preparation cost is higher preferably.Hide, meat are that fur-bearing animal is cultured main income, and blood, spleen generally do not have purposes, and the fur-bearing animal number of animals raised is big, bark fetching is concentrated, and is convenient to obtain cheap blood, spleen raw material during bark fetching, collects blood during bark fetching, and preparation antiserum cost is extremely low.Through the spleen of immune animal repeatedly, can be used for extracting special transfer factor, transfer factor has tangible immunoenhancement result.The immune programme for children of hyper-immune serum can determine that preparing antiserum and extracting transfer factor from spleen is mature technology by test, and route simply is easy to realize.
Summary of the invention:
The object of the present invention is to provide and a kind ofly obtain the blood that is used to prepare homologous antiserum in fur-bearing animal Vulpes, Nyctereutes procyonoides, the ermine body, be used to prepare the preparation method of the spleen of transfer factor, it makes full use of once depleted fur-bearing animal Vulpes, Nyctereutes procyonoides, Ermine blood liquid, spleen side-product, improves the fur-bearing animal by-product value.
Technical scheme of the present invention is achieved in that the preparation method of obtaining the blood that is used to prepare homologous antiserum in fur-bearing animal Vulpes, Nyctereutes procyonoides, the ermine body, being used to prepare the spleen of transfer factor, and it is characterized in that: concrete preparation method is: be that canine distemper virus, enteritis virus, adenovirus, hat virus diarrhoea, parainfluenza virus, pasteurellosis bacillus carry out antigen purification and concentrate to antigen at first 1;
2. in summer immunity once on the basis, booster immunization is three times before the bark fetching, select healthy individual, initial with preceding 45 days of bark fetching date, the 0th day to animal injection special transfer factor once, inoculation normal dose attenuated vaccine once inoculated once on the 10th day, the 24th day two multiple dose vaccine or inoculate once with homology tissue inactivation seedling; Canine distemper attenuated Seedling Vulpes one subnormal dosage of inoculation: 10
5.47TCID
50, Nyctereutes procyonoides once inoculates normal dose 10
5.47TCID
50, ermine once inoculates normal dose 10
5TCID
50The weak malicious Seedling Vulpes one subnormal dosage of inoculation of dog enteritis virus: 10
6.47TCID
50, Nyctereutes procyonoides one subnormal dosage of inoculation: 10
6.47TCID
50, the cats panleucopenia virus inactivated vaccine is once inoculated normal dose: 10
6TCID
50Adenovirus attenuated Seedling Vulpes one subnormal dosage of inoculation: 10
7TCID
50, Nyctereutes procyonoides one subnormal dosage of inoculation 10
7TCID
50The preparation of homology tissue inactivation seedling: the popular street strain of endemicity causes the animal morbidity, get spleen, the liver organization of morbidity animal, press normal saline volume/tissue weight than being 10:1 homogenate, fully freeze thawing is 4 times, the centrifugal 15min of 2000g gets supernatant and prepares aluminum hydroxide adjuvant formalin-inactivated Seedling;
3, during three booster immunizations, the management of strengthening feeding, guarantee to drink water; Blood sampling is taken a blood sample and was stopped raising in preceding 24 hours during 40～45 days, guarantees sufficient drinking-water; Hit dizzy, Baoding animal, from the aseptic anticoagulant blood-collecting of heart; With anticoagulation, preparation serum, be convenient to quick centrifugal collection blood plasma, reduce serum composition and pollute, improve the serum yield.Serum canine distemper neutralizing antibody (SN) is tired greater than 512, and adenovirus, enteritis parvovirus blood clotting suppress (HI) tires greater than 512, and it is antibiotic to add ten thousand/thimerosal, and packing-20 is ℃ frozen standby.Adult Vulpes, the about 200-150mL of Nyctereutes procyonoides blood sampling volume, serum 120mL; The blood sampling volume of ermine gets serum 30mL about 60mL.
Good effect of the present invention is: prepare homologous antiserum cheaply, transfer factor is for the early treatment of early treatment fur-bearing animal infectious disease, particularly viral disease, control epidemic situation spread powerful guarantee is provided; Large-scale fur-bearing animal breeding enterprise adopts this preparation homologous antiserum, transfer factor technology can form new treatment medicine, significantly improves culture benefit; Small-sized plant utilizes this technology, can be easy the sick serum of preparation infection; Provide safeguard for culturing the coming year to develop in a healthy way; Be used for the treatment of kind wild animal, a small amount of suspected case occur when raising colony, after cause of disease was made a definite diagnosis, emphasis was injected corresponding cause of disease height to the supposition health population and is exempted from antiserum, transfer factor, and the treatment their early stage is sick beastly, and the control epidemic situation spreads.
The specific embodiment:
The present invention will be further described below in conjunction with embodiment:
Be that canine distemper virus, enteritis parvovirus, adenovirus etc. carry out antigen purification and concentrate at first to antigen; Reduce volume injected, reduce the stress of animal.
Embodiment 1:
Ermine is selected healthy individual, and is initial with preceding 45 days of bark fetching date, and the 0th day to animal injection special transfer factor once, and inoculation normal dose attenuated vaccine once inoculated once on the 10th day, and the 24th day two multiple dose vaccine or the inoculation of homology tissue inactivation seedling are once; Canine distemper attenuated Seedling ermine one subnormal dosage of inoculation 10
5TCID
50Cats panleucopenia virus inactivated vaccine one subnormal dosage of inoculation: 10
6TCID
50The preparation of homology tissue inactivation seedling: the popular street strain of endemicity causes the animal morbidity, get spleen, the liver organization of morbidity animal, fully the freeze thawing tissue is 4 times, presses normal saline volume/tissue weight than being 10:1 homogenate, the centrifugal 15min of 2000g gets supernatant and prepares aluminum hydroxide adjuvant formalin-inactivated Seedling; During three booster immunizations, the management of strengthening feeding, the sufficient drinking-water of assurance; Take a blood sample during 40-45 days, take a blood sample and stopped raising in preceding 24 hours, guarantee drinking-water; Hit dizzy, Baoding animal, from the aseptic anticoagulant blood-collecting of heart; With anticoagulation, preparation serum, be convenient to quick centrifugal collection blood plasma, reduce serum composition and pollute, improve the serum yield; Serum canine distemper neutralizing antibody (SN) is tired greater than 512, and enteritis parvovirus blood clotting suppresses (H I) tires greater than 512, and it is antibiotic to add ten thousand/thimerosal, and packing-20 is ℃ frozen standby.The blood sampling volume of an adult ermine gets serum 30mL about 60mL.
Embodiment 2:
Vulpes is selected healthy individual, and is initial with preceding 45 days of bark fetching date, and the 0th day to animal injection special transfer factor once, and inoculation normal dose attenuated vaccine once inoculated once on the 10th day, the 24th day two multiple dose vaccine or inoculate once with homology tissue inactivation seedling; Canine distemper attenuated Seedling Vulpes one subnormal dosage of inoculation: 10
5.47TCID
50, the weak malicious Seedling Vulpes one subnormal dosage of inoculation of dog enteritis virus: 10
6.47TCID
50, adenovirus attenuated Seedling Vulpes one subnormal dosage of inoculation: 10
7TCID
50The preparation of homology tissue inactivation seedling: the popular street strain of endemicity causes the animal morbidity, the spleen, liver organization of getting the morbidity animal by volume/weight ratio is 10:1, homogenate, fully freeze thawing is 4 times, the centrifugal 15min of 2000g gets supernatant and prepares aluminum hydroxide adjuvant formalin-inactivated Seedling.During three booster immunizations, the management of strengthening feeding, assurance drinking-water; Blood sampling is taken a blood sample and was stopped raising in preceding 24 hours during 40～45 days, guarantees sufficient drinking-water, hits dizzy, Baoding animal, from the aseptic anticoagulant blood-collecting of heart; With anticoagulation, preparation serum, be convenient to quick centrifugal collection blood plasma, reduce serum composition and pollute, improve the serum yield; Serum canine distemper neutralizing antibody (SN) is tired greater than 512, and adenovirus, enteritis parvovirus blood clotting suppress (H I) tires greater than 512, and it is antibiotic to add ten thousand/thimerosal, and packing-20 is ℃ frozen standby.About 200～the 150mL of adult Vulpes blood sampling volume gets serum 120mL.
Embodiment 3:
Nyctereutes procyonoides is selected healthy individual, and is initial with preceding 45 days of bark fetching date, and the 0th day, once inoculate the normal dose attenuated vaccine once to animal injection special transfer factor, inoculated once in the 10th day, the 24th day two multiple dose vaccine or the inoculation of homology tissue inactivation seedling are once; Canine distemper attenuated Seedling Nyctereutes procyonoides one subnormal dosage of inoculation 10
5.47TCID
50The weak malicious Seedling Nyctereutes procyonoides one subnormal dosage of inoculation of dog enteritis virus: 10
6.47TCID
50Adenovirus attenuated Seedling Nyctereutes procyonoides one subnormal dosage of inoculation 10
7TCID
50The preparation of homology tissue inactivation seedling: the popular street strain of endemicity causes the animal morbidity, get spleen, the liver organization of morbidity animal, fully the freeze thawing tissue is 4 times, presses normal saline volume/tissue weight than being 10:1 homogenate, the centrifugal 15min of 2000g gets supernatant and prepares aluminum hydroxide adjuvant formalin-inactivated Seedling.During three booster immunizations, the management of strengthening feeding, assurance drinking-water; Blood sampling is taken a blood sample and was stopped raising in preceding 24 hours during 40～45 days, guarantees sufficient drinking-water; Hit dizzy, Baoding animal, from the aseptic anticoagulant blood-collecting of heart; With anticoagulation, preparation serum, be convenient to quick centrifugal collection blood plasma, reduce serum composition and pollute, improve the serum yield.Serum canine distemper neutralizing antibody (SN) is tired greater than 512, and adenovirus, enteritis parvovirus blood clotting suppress (H I) tires greater than 512, and it is antibiotic to add ten thousand/thimerosal, and packing-20 is ℃ frozen standby.About 200～the 150mL of adult Nyctereutes procyonoides blood sampling volume gets serum 120mL.
Claims (1)
- Obtain the blood that is used to prepare homologous antiserum in fur-bearing animal Vulpes, Nyctereutes procyonoides, the ermine body, be used to prepare the preparation method of the spleen of transfer factor, it is characterized in that: concrete preparation method is:
(1). be that canine distemper virus, enteritis virus, adenovirus, diarrhoea coronavirus, parainfluenza virus, pasteurellosis bacillus carry out purification and concentrate at first, reduce volume injected, reduce the stress of animal antigen;(2). in summer immunity once on the basis, booster immunization is three times before the bark fetching; Select healthy individual, initial with preceding 45 days of bark fetching date, the 0th day to animal injection special transfer factor once, and inoculation normal dose attenuated vaccine once inoculated once on the 10th day, the 24th day two multiple dose vaccine or inoculate once with homology tissue inactivation seedling; Canine distemper attenuated Seedling Vulpes one subnormal dosage of inoculation: 10 5.47TCID 50, Nyctereutes procyonoides one subnormal dosage of inoculation 10 5.47TCID 50, ermine one subnormal dosage of inoculation 10 5TCID 50The weak malicious Seedling Vulpes one subnormal dosage of inoculation of dog enteritis virus: 10 6.47TCID 50, Nyctereutes procyonoides once inoculates normal dose 10 6.47TCID 50, the cats panleucopenia virus inactivated vaccine is once inoculated normal dose 10 6TCID 50Adenovirus attenuated Seedling Vulpes one subnormal dosage of inoculation: 10 7TCID 50, Nyctereutes procyonoides once inoculates normal dose 10 7TCID 50The preparation of homology tissue inactivation seedling: the popular street strain of endemicity causes the animal morbidity, get spleen, liver or the intestinal tissue of morbidity animal, fully the freeze thawing tissue is 4 times, press normal saline volume/tissue weight than being 10:1 homogenate, the centrifugal 15min of 2000g gets supernatant and prepares aluminum hydroxide adjuvant formalin-inactivated Seedling;(3). during three booster immunizations, the management of strengthening feeding, assurance drinking-water; Blood sampling is taken a blood sample and was stopped raising in preceding 24 hours during 40～45 days, guarantees sufficient drinking-water; Hit dizzy, Baoding animal, from the aseptic anticoagulant blood-collecting of heart; With anticoagulation, preparation serum, be convenient to quick centrifugal collection serum, reduce serum composition and pollute, improve the serum yield; Serum canine distemper neutralizing antibody (SN) is tired greater than 512, and adenovirus, enteritis parvovirus blood clotting suppress (HI) tires greater than 512, and it is antibiotic to add ten thousand/thimerosal, and packing-20 is ℃ frozen standby; Adult Vulpes, the about 200～150mL of Nyctereutes procyonoides blood sampling volume, serum 120mL; The blood sampling volume of ermine gets the about 30mL of serum about 60mL.
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| CNA2008100510935A CN101422485A (en) | 2008-08-15 | 2008-08-15 | Preparation method of spleen byproducts for producing homology anti-serum blood and transfer factor from fox, raccoon dog, mink |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102580079A (en) * | 2012-03-30 | 2012-07-18 | 河南农业大学 | Porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine and preparation method thereof |
| CN104587460A (en) * | 2014-12-25 | 2015-05-06 | 华威特（北京）生物科技有限公司 | Mink viral enteritis and canine distemper binary living vaccine as well as preparation method and application thereof |
| CN107881154A (en) * | 2017-12-26 | 2018-04-06 | 中国农业科学院特产研究所 | Raccoon dog parvovirus attenuated vaccine strain and its purposes in Raccoon dog parvovirus attenuated vaccine is prepared |
| CN111184741A (en) * | 2020-02-20 | 2020-05-22 | 张宏亮 | Preparation method of biological agent for treating virus infected person, related biological agent and application |
-
2008
- 2008-08-15 CN CNA2008100510935A patent/CN101422485A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102580079A (en) * | 2012-03-30 | 2012-07-18 | 河南农业大学 | Porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine and preparation method thereof |
| CN102580079B (en) * | 2012-03-30 | 2015-09-23 | 河南农业大学 | Porcine parvovirus nanometer alumina gel adjuvant inactivated vaccine and preparation method thereof |
| CN104587460A (en) * | 2014-12-25 | 2015-05-06 | 华威特（北京）生物科技有限公司 | Mink viral enteritis and canine distemper binary living vaccine as well as preparation method and application thereof |
| CN104587460B (en) * | 2014-12-25 | 2016-09-21 | 华威特（江苏）生物制药有限公司 | Mink viral enteritis, canine distemper bigeminal live vaccine and its preparation method and application |
| CN107881154A (en) * | 2017-12-26 | 2018-04-06 | 中国农业科学院特产研究所 | Raccoon dog parvovirus attenuated vaccine strain and its purposes in Raccoon dog parvovirus attenuated vaccine is prepared |
| CN107881154B (en) * | 2017-12-26 | 2021-03-05 | 中国农业科学院特产研究所 | Raccoon parvovirus attenuated vaccine strain and application thereof in preparation of raccoon parvovirus attenuated vaccine |
| CN111184741A (en) * | 2020-02-20 | 2020-05-22 | 张宏亮 | Preparation method of biological agent for treating virus infected person, related biological agent and application |
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