CN101418319B - Method for producing alcohol by using stalk resource - Google Patents
Method for producing alcohol by using stalk resource Download PDFInfo
- Publication number
- CN101418319B CN101418319B CN2008102288662A CN200810228866A CN101418319B CN 101418319 B CN101418319 B CN 101418319B CN 2008102288662 A CN2008102288662 A CN 2008102288662A CN 200810228866 A CN200810228866 A CN 200810228866A CN 101418319 B CN101418319 B CN 101418319B
- Authority
- CN
- China
- Prior art keywords
- holocellulose
- percent
- alcohol
- cellulose
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 title abstract description 3
- 238000000034 method Methods 0.000 claims abstract description 16
- 239000001913 cellulose Substances 0.000 claims abstract description 15
- 229920002678 cellulose Polymers 0.000 claims abstract description 15
- 239000010902 straw Substances 0.000 claims abstract description 10
- 238000004821 distillation Methods 0.000 claims abstract description 9
- 238000000855 fermentation Methods 0.000 claims abstract description 9
- 230000004151 fermentation Effects 0.000 claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 8
- 239000002054 inoculum Substances 0.000 claims abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 7
- 241000499912 Trichoderma reesei Species 0.000 claims abstract description 4
- 150000001875 compounds Chemical class 0.000 claims abstract description 3
- 239000002994 raw material Substances 0.000 claims description 16
- 238000004321 preservation Methods 0.000 claims description 6
- 150000002972 pentoses Chemical class 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 2
- 238000010563 solid-state fermentation Methods 0.000 claims description 2
- 239000008399 tap water Substances 0.000 claims description 2
- 235000020679 tap water Nutrition 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 abstract description 4
- 102000004190 Enzymes Human genes 0.000 abstract description 4
- 239000000446 fuel Substances 0.000 abstract description 4
- 238000002360 preparation method Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract 3
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 238000009413 insulation Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 5
- 108010059892 Cellulase Proteins 0.000 description 4
- 229940106157 cellulase Drugs 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000002349 favourable effect Effects 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a method for producing alcohol by utilizing straw resources. In the method, holocellulose in cellulose prepared by crop straw is taken as a material, the purity of the holocellulose is between 75 and 90 percent, of which, the cellulose content is between 55 and 80 percent, and the pentosan content is between 5 and 15 percent; the material is crushed to be 40 meshes for standby; enzymolysis: according to the enzyme receiving amount of 1.5 to 3.5 percent, Trichoderma reesei compound cellulose enzyme preparation is added to the crushed material, and the mixture is subjected to enzymolysis at a temperature of between 35 and 50 DEG C for 4 to 6 days; and solid fermentation: according to the inoculum amount of 5 to 10 percent, Angel active dry yeast is added into the mixture and the mixture is subjected to thermal insulation fermentation at a temperature of 40 DEG C for 2 to 4 days; and 95 percent alcohol is obtained by distillation. The method has the advantages of converting agriculture and forestry waste into alcohol fuel, having simple technique, forming industrialized utilization, being quite suitable for the situation in China, being very profitable from theenergy safety perspective, and eliminating the environmental pollution problem caused by the straw burning.
Description
Technical field
The present invention relates to the technology of the application of a kind of biological enzyme in stalk resource production alcohol, belong to the biomass energy field.
Background technology
Fuel alcohol can be inexhausted as renewable energy source, and can not cause Greenhouse effect.Microbial fermentation sugar can be produced alcohol.Produce the microbial host yeast saccharomyces cerevisiae and the zymomonas mobilis of alcohol if in industrial production, be used at present ferment.The biomass that contain glucide that comprise stalk all may be as the raw material of zymamsis, and the utilization of macromolecular substance needs the degraded of process enzyme earlier.Biological alcohol is as the surrogate of oil, and its industrial chain is also continuing extension.There is development Mierocrystalline cellulose system alcoholic acid favourable condition in China, every year, only agricultural crop straw just had more than 700,000,000 ton (dry weight), and China's grain resource is not abundant, therefore agriculture and forestry organic waste material is converted into alcohol fuel, the formation industrialization utilizes, be fit to very much the national conditions of China, also be very favourable from the energy security angle, and can eliminate the environmental problem that causes by crop straw burning.
Summary of the invention
The holocellulose that the objective of the invention is to obtain through alkaline process or liquefaction process in the agricultural crop straw system Mierocrystalline cellulose is a main raw material, handles through biotechnology, produces biological cheaply alcohol.
The objective of the invention is to realize by following technical proposal:
(1) raw material is prepared: with the holocellulose in the agricultural crop straw system Mierocrystalline cellulose is raw material, and the purity of holocellulose is 75～90%, and wherein cellulose 55～80%, poly-pentose 5～15%.It is standby that it is crushed to 40 orders.
Can utilize through alkaline process and liquefaction process with agricultural crop straw system Mierocrystalline cellulose and to prepare holocellulose;
Wherein a) utilize holocellulose raw material through the alkaline process preparation; Holocellulose purity is 85%, and wherein cellulose 65～77%, poly-pentose 8～14%.
B) the holocellulose raw material that utilizes liquefaction process to prepare, holocellulose purity 78%, wherein cellulose 58～68%, poly-pentose 5～15%.
(2) enzymolysis: get above-mentioned a) or b) raw material 60～85% add tap water by it, press the compound cellulose zymin (Trichoderma reesei) that 1.5～3.5% of material quantity inserts the Trichodermareeseis products, 35～50 ℃ of enzymolysis 4～6 days.
(3) solid state fermentation: add Angel active dry yeast bacterium by 5～10% inoculum size, 40 ± 1 ℃ of heat-preservation fermentations 2～4 days.
(4) distillation: the fermented product distillation obtains 95% alcohol, 0.19～0.25kg.
The present invention is converted into alcohol fuel with agriculture and forestry organic waste material, and technology is simple, formation industrialization utilization, is fit to very much the national conditions of China, also is very favourable from the energy security angle, and can eliminates the environmental problem that is caused by crop straw burning.
Embodiment
Embodiment 1
Paper industry 1 kilogram of holocellulose of obtaining after alkalization adds water 65%, by 2% ratio adding complex cellulase of raw material, and 45 ℃ of enzymolysis 5 days.Add yeast by 10% inoculum size then,, obtaining 95% alcohol 0.20kg through distillation 40 ± 1 ℃ of heat-preservation fermentations 3 days.
Embodiment 2
1 kilogram of the holocellulose that paper industry obtains through alkaline cooking adds water 70%, adds complex cellulase in 3% ratio of raw material, 45 ℃ of enzymolysis 5 days.Add yeast by 10% inoculum size then,, obtaining 95% alcohol 0.22kg through distillation 40 ℃ ± 1 ℃ heat-preservation fermentation 3 days.
Embodiment 3
1 kilogram of the holocellulose that the paper industry liquefaction process obtains adds water 75%.2% ratio in raw material adds complex cellulase, 45 ℃ of enzymolysis 5 days.Add yeast by 10% inoculum size then,, obtaining 95% alcohol 0.19kg through distillation 40 ℃ ± 1 ℃ heat-preservation fermentation 3 days.
Embodiment 4
1 kilogram of the holocellulose that the paper industry liquefaction process obtains adds water 75%.3% ratio in raw material adds complex cellulase, 45 ℃ of enzymolysis 5 days.Add yeast by 10% inoculum size then,, obtaining 95% alcohol 0.21kg through distillation 40 ± 1 ℃ of heat-preservation fermentations 3 days.
Claims (1)
1. utilize stalk resource to produce the method for alcohol, it is characterized in that processing step is:
(1) raw material is prepared: with the holocellulose in the agricultural crop straw system Mierocrystalline cellulose is raw material, and wherein said holocellulose is respectively:
A) utilize the holocellulose raw material for preparing through alkaline process; Holocellulose purity is 85%, and wherein cellulose 65～77%, poly-pentose 8～14%; It is standby that it is crushed to 40 orders; Or
B) the holocellulose raw material that utilizes liquefaction process to prepare, holocellulose purity 78%, wherein cellulose 58～68%, poly-pentose 5～15%; It is standby that it is crushed to 40 orders;
(2) enzymolysis: get above-mentioned a) or b) raw material add tap water by its 60～80% amount, press the compound cellulose zymin that 1.5～3.5% of material quantity inserts the Trichodermareeseis products, 35～50 ℃ of enzymolysis 4～6 days;
(3) solid state fermentation: add Angel active dry yeast bacterium by 5～10% inoculum size, 40 ± 1 ℃ of heat-preservation fermentations 2～4 days;
(4) distillation: the fermented product distillation obtains 95% alcohol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102288662A CN101418319B (en) | 2008-11-18 | 2008-11-18 | Method for producing alcohol by using stalk resource |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102288662A CN101418319B (en) | 2008-11-18 | 2008-11-18 | Method for producing alcohol by using stalk resource |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101418319A CN101418319A (en) | 2009-04-29 |
| CN101418319B true CN101418319B (en) | 2011-07-20 |
Family
ID=40629321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102288662A Expired - Fee Related CN101418319B (en) | 2008-11-18 | 2008-11-18 | Method for producing alcohol by using stalk resource |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101418319B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327068A (en) * | 2000-06-02 | 2001-12-19 | 唐山市冀东溶剂厂 | Process for producing alcohol with xylose slag |
| CN101024847A (en) * | 2007-04-06 | 2007-08-29 | 山东大学 | Method for producing alcohol and feed by utilizing seaweed chemical waste material |
| CN101298744A (en) * | 2008-06-04 | 2008-11-05 | 大连工业大学 | Method for stalk liquefaction preprocessing to prepare fuel ethyl hydrate raw material |
-
2008
- 2008-11-18 CN CN2008102288662A patent/CN101418319B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327068A (en) * | 2000-06-02 | 2001-12-19 | 唐山市冀东溶剂厂 | Process for producing alcohol with xylose slag |
| CN101024847A (en) * | 2007-04-06 | 2007-08-29 | 山东大学 | Method for producing alcohol and feed by utilizing seaweed chemical waste material |
| CN101298744A (en) * | 2008-06-04 | 2008-11-05 | 大连工业大学 | Method for stalk liquefaction preprocessing to prepare fuel ethyl hydrate raw material |
Non-Patent Citations (2)
| Title |
|---|
| 夏黎明等.应用固定化里氏木霉糖化玉米秆纤维素的研究.《微生物学报》.1998,第38卷(第2期),摘要、第114页倒数第15行至第倒数第9行、1.5部分、2.2部分. * |
| 陶荣等.未来可持续发展的新动力-秸秆发酵制酒精.《酿酒》.2006,第33卷(第3期),45-48. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101418319A (en) | 2009-04-29 |
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Owner name: HAINAN UNIVERSITY Free format text: FORMER OWNER: DALIAN POLYTECHNIC UNIVERSITY Effective date: 20140227 |
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Effective date of registration: 20140227 Address after: 570228 School of materials and chemical engineering, Hainan University, 58 Renmin Road, Hainan, Haikou Patentee after: Hainan University Address before: 116034 Ganjingzi Light Industry Zone, Liaoning, No. 1, No. Patentee before: Dalian Polytechnic University |
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Granted publication date: 20110720 Termination date: 20201118 |