CN101417990B - Method for extracting burdock leaf flavonoids - Google Patents
Method for extracting burdock leaf flavonoids Download PDFInfo
- Publication number
- CN101417990B CN101417990B CN2008102288681A CN200810228868A CN101417990B CN 101417990 B CN101417990 B CN 101417990B CN 2008102288681 A CN2008102288681 A CN 2008102288681A CN 200810228868 A CN200810228868 A CN 200810228868A CN 101417990 B CN101417990 B CN 101417990B
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- Prior art keywords
- ethyl acetate
- extraction
- extracting
- evaporation
- burdock
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- 229930003935 flavonoid Natural products 0.000 title claims abstract description 17
- 235000017173 flavonoids Nutrition 0.000 title claims abstract description 17
- 235000003130 Arctium lappa Nutrition 0.000 title claims abstract description 15
- 235000008078 Arctium minus Nutrition 0.000 title claims abstract description 14
- 150000002215 flavonoids Chemical class 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims abstract description 11
- 240000005528 Arctium lappa Species 0.000 title description 8
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 33
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000000605 extraction Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 241000208843 Arctium Species 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 3
- 238000010992 reflux Methods 0.000 claims abstract description 3
- 238000001704 evaporation Methods 0.000 claims abstract 7
- 230000008020 evaporation Effects 0.000 claims abstract 6
- XOJVHLIYNSOZOO-SWOBOCGESA-N Arctiin Chemical compound C1=C(OC)C(OC)=CC=C1C[C@@H]1[C@@H](CC=2C=C(OC)C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)=CC=2)C(=O)OC1 XOJVHLIYNSOZOO-SWOBOCGESA-N 0.000 claims description 10
- 238000003810 ethyl acetate extraction Methods 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims 1
- 230000006837 decompression Effects 0.000 claims 1
- MHYCRLGKOZWVEF-UHFFFAOYSA-N ethyl acetate;hydrate Chemical compound O.CCOC(C)=O MHYCRLGKOZWVEF-UHFFFAOYSA-N 0.000 claims 1
- 238000010025 steaming Methods 0.000 claims 1
- -1 flavonoid compound Chemical class 0.000 abstract description 6
- 229930014626 natural product Natural products 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract 3
- 238000002386 leaching Methods 0.000 abstract 2
- 239000012071 phase Substances 0.000 abstract 2
- 239000008346 aqueous phase Substances 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 230000003068 static effect Effects 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 206010022489 Insulin Resistance Diseases 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 230000003035 anti-peroxidant effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000002440 hepatic effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to a method for extracting burdock leaf flavonoid. The extraction process comprises the following: (1) a step of raw material processing, which is to clean, dry and crush burdock leaves, and soak the burdock leaves with 60 to 95 percent ethanol solution according to the solid-liquid ratio of between 1 to 15 and 1 to 40 at room temperature for 12 to 48 hours; (2) a step of preparation of a leaching liquor, which is to heat and reflux the mixture for 3 and 6 hours, cool the mixture to the room temperature and filter the mixture; (3) a step of concentration, which is to distill off the ethanol in the leaching liquor to obtain a concentrated solution; (4) a step of extraction, which comprises two steps: 1) extracting chloroform by extracting the concentrated solution for2 to 3 times with the chloroform and combining an aqueous phase; and 2) extracting ethyl acetate used for the water phase for 2 to 3 times and combining an ethyl acetate phase; and (5) a step of evaporation, which is to remove the ethyl acetate through evaporation so as to obtain the burdock leaf flavonoid. The method is very effective, simple and safe in the extraction and the separation of a natural product of the flavonoid compound, and is easy to realize industrial production.
Description
Technical field
The present invention relates to extracting effective components technology from plant, particularly extract the technical field of flavonoid compound from Herba Arctii leaf.
Background technology
Burdock (Arctium lappa L.), another name are disliked real root, the glutinous root of mouse, ox dish etc., belong to the composite family Arctium, and 2 years these plants of sward are product with the fleshy root.Fleshy root is elongated cylindrical, and length is generally 60～100cm, skin is yellowish-brown, dark brown isochrome, and the meat canescence is thick and stiff slightly, and dietotherapeutic, and widely distributed in China the comparative advantages of natural resource are arranged, and cultivated area is also in continuous expansion.And the nutrition content height in the Herba Arctii leaf, and every nutrition equilibrium degree is good, utility value is high.
Contain a large amount of flavonoid compounds in the Herba Arctii leaf extracting solution.Flavonoid compound have good hypoglycemic, transfer effects such as blood fat, anti peroxidation of lipid, increase Regular Insulin and acceptor binding force, and can reduce fasting plasma glucose, improve sugar tolerance, can reduce lipid peroxidation product mda content in serum and the hepatic tissue, and superoxide dismutase activity in the increase serum, increase a lot of physiological functions such as insulin sensitivity index.But still do not have the report of effective extracting method and do not see the information that this type of launch is arranged at present.
Summary of the invention
The objective of the invention is to Herba Arctii leaf is raw material, through the ethanol extraction, extracts flavonoid compound.
The objective of the invention is to realize through the following steps:
(a) with after Herba Arctii leaf cleaning, oven dry, pulverizing, itself and 60～95% ethanolic solns are placed reaction vessel by solid-to-liquid ratio 1:15～40, soak at room temperature 12～48h.
(b) with the material reflux 3～6h of (a) step, obtain vat liquor.
(c) obtain concentrated solution after the vat liquor that (b) step is made steams ethanolic soln.
(d) concentrated solution that (c) step is obtained moves into separating funnel, adds chloroform extraction 2～3 times, each 30ml, and static layering, the water that obtains merges uses ethyl acetate extraction 2～3 times again, each 25ml.The combined ethyl acetate extraction liquid steams the burdock leaf flavonoids that is that ethyl acetate obtains through Rotary Evaporators.
(e) the gained sample is measured with ultraviolet spectrophotometer method.
The ethanol lixiviate that the present invention takes, chloroform extraction, be a kind of very effective method with the method for ethyl acetate extraction for the extraction separation of natural product flavonoid compound again.This method is produced simple, safety, is easy to realize suitability for industrialized production.
The invention will be further described with embodiment below.
Embodiment
Embodiment 1
The Herba Arctii leaf 100g of drying and crushing is in advance mixed by solid-to-liquid ratio 1:30 with 95% ethanolic soln, at 70 ℃ of following lixiviate 3h of extraction temperature, soak body fluid chloroform extraction 3 times, each 30ml, water merges after the static layering, and water is again through ethyl acetate extraction 3 times, each 25ml, the combined ethyl acetate extraction liquid steams ethyl acetate through Rotary Evaporators.Be burdock leaf flavonoids 3.2mg.
Embodiment 2
The Herba Arctii leaf 100g of drying and crushing is in advance mixed by solid-to-liquid ratio 1:20 with 80% ethanolic soln, at 70 ℃ of following lixiviate 3h of extraction temperature, soak body fluid chloroform extraction 3 times, each 30ml, water merges after the static layering, and water is through ethyl acetate extraction 3 times, each 25ml, the combined ethyl acetate extraction liquid is burdock leaf flavonoids 4.8mg through what Rotary Evaporators steamed that ethyl acetate obtains.
Embodiment 3
The Herba Arctii leaf 100g of drying and crushing is in advance mixed by solid-to-liquid ratio 1:20 with 70% ethanolic soln, at 80 ℃ of following lixiviate 4h of extraction temperature, chloroform extraction 3 times, each 30ml.Water merges after the static layering, and water is through ethyl acetate extraction, each 25ml, and the combined ethyl acetate extraction liquid is burdock leaf flavonoids 8.7mg through what Rotary Evaporators steamed that ethyl acetate obtains.
Claims (2)
1. the extracting method of burdock leaf flavonoids is characterized in that extraction process is:
(1) raw material is handled: after Herba Arctii leaf is cleaned, dries, pulverizes, itself and 60～95% ethanolic solns are placed reaction vessel by solid-to-liquid ratio 1:15～40, soak at room temperature 12～48h;
(2) producing of vat liquor: with its 3～6h that refluxes that heats, be cooled to room temperature, filter;
(3) concentrate: the vat liquor steaming removes ethanol and gets concentrated solution;
(4) extract: 1. chloroform extraction: concentrated solution is with chloroform extraction 2～3 times, each 30ml, and water merges; 2. water ethyl acetate extraction 2～3 times, each 25ml, combined ethyl acetate phase;
(5) evaporation: evaporation is removed ethyl acetate and is got burdock leaf flavonoids.
2. the extracting method of burdock leaf flavonoids according to claim 1 is characterized in that evaporating in extraction process (5) evaporation step and removes in the ethyl acetate, and described evaporation is to use the rotatory evaporator decompression to evaporate down and removes ethyl acetate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102288681A CN101417990B (en) | 2008-11-18 | 2008-11-18 | Method for extracting burdock leaf flavonoids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102288681A CN101417990B (en) | 2008-11-18 | 2008-11-18 | Method for extracting burdock leaf flavonoids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101417990A CN101417990A (en) | 2009-04-29 |
| CN101417990B true CN101417990B (en) | 2011-05-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102288681A Expired - Fee Related CN101417990B (en) | 2008-11-18 | 2008-11-18 | Method for extracting burdock leaf flavonoids |
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| CN (1) | CN101417990B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103071021A (en) * | 2012-10-24 | 2013-05-01 | 内蒙古农业大学 | Method for extracting flavonoids from Allium mongolicum Regel leaves |
| CN102935091A (en) * | 2012-11-19 | 2013-02-20 | 广西蜜博士蜂业有限责任公司 | Propolis flavonoid extracting method |
| CN106265813A (en) * | 2016-09-23 | 2017-01-04 | 杭州富阳佳畅机械有限公司 | A kind of extracting method of Radix Arctii total flavones |
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- 2008-11-18 CN CN2008102288681A patent/CN101417990B/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN101417990A (en) | 2009-04-29 |
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