CN101412746A - Preparation of feather protein film - Google Patents
Preparation of feather protein film Download PDFInfo
- Publication number
- CN101412746A CN101412746A CNA2008101625264A CN200810162526A CN101412746A CN 101412746 A CN101412746 A CN 101412746A CN A2008101625264 A CNA2008101625264 A CN A2008101625264A CN 200810162526 A CN200810162526 A CN 200810162526A CN 101412746 A CN101412746 A CN 101412746A
- Authority
- CN
- China
- Prior art keywords
- feather
- oven dry
- dissolving
- film
- protein film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000003746 feather Anatomy 0.000 title claims abstract description 51
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title description 2
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000005406 washing Methods 0.000 claims abstract description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 10
- BYEAHWXPCBROCE-UHFFFAOYSA-N 1,1,1,3,3,3-hexafluoropropan-2-ol Chemical compound FC(F)(F)C(O)C(F)(F)F BYEAHWXPCBROCE-UHFFFAOYSA-N 0.000 claims description 6
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000004793 Polystyrene Substances 0.000 claims description 5
- 229920002223 polystyrene Polymers 0.000 claims description 5
- 239000012460 protein solution Substances 0.000 claims description 5
- 239000003513 alkali Substances 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000004090 dissolution Methods 0.000 abstract 1
- 235000017550 sodium carbonate Nutrition 0.000 description 5
- 238000001035 drying Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000009374 poultry farming Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Fertilizers (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a method for preparing protein film of feather. Firstly, alkali is adopted to carry out degumming on the feather; secondly, the feather is dissolved at high temperature; and finally, the feather is subjected to film forming in a constant temperature and dampness box to obtain the needed protein film of the feather. The method comprises the following process flows: the feather is subjected to feather rod shaving, cleaning, alkali degumming, washing, high-temperature dissolution and film forming. The method provides a new thought for the utilization of the waste feather and has a simple process; and the prepared film is hopefully used for skin rehabilitation and other aspects.
Description
Technical field
The present invention relates to a kind of preparation method of protein film, particularly relate to a kind of preparing protein film of feather.
Background technology
In China,, there is every year a large amount of feathers to go out of use or well do not utilized along with the quantity of poultry farming increases.How to turn waste into wealth is the topic that everybody pays close attention to, in Chinese patent 200610051886.8, disclose to utilize lichen spore bacillus ZJUEL 31410 that feather is carried out microbiological deterioration, then to the degraded product as feed.Add some beneficial organism floras, water in Chinese patent 200810028177.7, disclosing with feather--stir,----deodorizing--drying--moulding, packing are used as novel biological fertilizer with the product that obtains in quality inspection at sealed fermenting.But seldom relevant for feather protein is studied as biomaterial.
Summary of the invention
The purpose of this invention is to provide a kind of preparing protein film of feather, feather protein as biomaterial.
Technical process of the present invention is: feather is shaved the defeathering bar--cleans----washing--high-temperature digestion---film forming that alkali lye comes unstuck.
Below technology of the present invention is described in further detail:
(1) with the feather decon, shaves bar, the oven dry of washing back;
(2) in the feather after oven dry, adding mass percent is the 1-2% sodium carbonate solution, handles 1-2 hour at 70-80 ℃, washes totally feather after oven dry obtains coming unstuck then with water;
(3) in the feather after the oven dry of coming unstuck, adding concentration is 20-40gL
-1Hexafluoroisopropanol, in 120-140 ℃ the dissolving 3-4 hour, the settled solution after obtaining dissolving;
(4) the feather protein solution after will dissolving is poured in the polystyrene culture dish, the smooth bubble that goes; At 30 ℃, relative humidity is to place 3 days in 65% climatic chamber, obtains feather protein film.
In above each operation:
1, be in order to deviate from a large amount of colloid in feather surface with yellow soda ash.
2, be in order to make the feather protein molecule obtain disperseing and dissolving with hexafluoroisopropanol.
The beneficial effect that the present invention has is:
The present invention not only proposes new thinking to the utilization of a large amount of waste feathers, and simple, the prepared film of technology is expected to be used for aspects such as skin repair.
Embodiment
Embodiment 1:
(1) with the feather decon, shaves bar, the oven dry of washing back;
(2) feather after will drying takes by weighing 10g and puts into the 500mL beaker, and adding the 200mL mass percent is 1% sodium carbonate solution, handles 1.5 hours at 80 ℃, wash with water then clean, the oven dry back feather that obtains coming unstuck;
(3) feather that will come unstuck after the oven dry is got 2g and is put into the 100mL beaker, and adding 50mL concentration is 40g.L
-1Hexafluoroisopropanol in 120 ℃ the dissolving 4 hours, the settled solution after obtaining dissolving;
(4) the feather protein solution after will dissolving is got 10mL to put into diameter is the 12cm polystyrene culture dish, the smooth bubble that goes; At 30 ℃, relative humidity is to place 3 days in 65% climatic chamber, obtains feather protein film.
Embodiment 2:
(1) with the feather decon, shaves bar, the oven dry of washing back;
(2) feather after will drying takes by weighing 10g and puts into the 500mL beaker, and adding the 200mL mass percent is 2% sodium carbonate solution, handles 2 hours at 70 ℃, wash with water then clean, the oven dry back feather that obtains coming unstuck;
(3) feather that will come unstuck after the oven dry is got 2g and is put into the 100mL beaker, and adding 50mL concentration is 30g.L
-1Hexafluoroisopropanol in 140 ℃ the dissolving 3 hours, the settled solution after obtaining dissolving;
(4) the feather protein solution after will dissolving is got 10mL to put into diameter is the 12cm polystyrene culture dish, the smooth bubble that goes; At 30 ℃, relative humidity is to place 3 days in 65% climatic chamber, obtains feather protein film.
Embodiment 3:
(1) with the feather decon, shaves bar, the oven dry of washing back;
(2) feather after will drying takes by weighing 10g and puts into the 500mL beaker, and adding the 200mL mass percent is 1.5% sodium carbonate solution, handles 1 hour at 75 ℃, wash with water then clean, the oven dry back feather that obtains coming unstuck;
(3) feather that will come unstuck after the oven dry is got 2g and is put into the 100mL beaker, and adding 50mL concentration is 20g.L
-1Hexafluoroisopropanol in 130 ℃ the dissolving 3.5 hours, the settled solution after obtaining dissolving;
(4) the feather protein solution after will dissolving is got 10mL to put into diameter is the 12cm polystyrene culture dish, the smooth bubble that goes; At 30 ℃, relative humidity is to place 3 days in 65% climatic chamber, obtains feather protein film.
Claims (1)
1, a kind of preparing protein film of feather is characterized in that, the step of this method is as follows:
(1) with the feather decon, shaves bar, the oven dry of washing back;
(2) in the feather after oven dry, adding mass percent is the 1-2% sodium carbonate solution, handles 1-2 hour at 70-80 ℃, washes totally feather after oven dry obtains coming unstuck then with water;
(3) in the feather after the oven dry of coming unstuck, adding concentration is 20-40gL
-1Hexafluoroisopropanol, in 120-140 ℃ the dissolving 3-4 hour, the settled solution after obtaining dissolving;
(4) the feather protein solution after will dissolving is poured in the polystyrene culture dish, the smooth bubble that goes; At 30 ℃, relative humidity is to place 3 days in 65% climatic chamber, obtains feather protein film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101625264A CN101412746A (en) | 2008-12-01 | 2008-12-01 | Preparation of feather protein film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101625264A CN101412746A (en) | 2008-12-01 | 2008-12-01 | Preparation of feather protein film |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101412746A true CN101412746A (en) | 2009-04-22 |
Family
ID=40593508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008101625264A Pending CN101412746A (en) | 2008-12-01 | 2008-12-01 | Preparation of feather protein film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101412746A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102585277A (en) * | 2012-02-16 | 2012-07-18 | 武汉纺织大学 | Process for preparing keratin porous membrane by refrigerating |
| CN108373544A (en) * | 2018-02-06 | 2018-08-07 | 安徽工程大学 | A kind of preparation method of nanometer of feather down azelon film |
-
2008
- 2008-12-01 CN CNA2008101625264A patent/CN101412746A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102585277A (en) * | 2012-02-16 | 2012-07-18 | 武汉纺织大学 | Process for preparing keratin porous membrane by refrigerating |
| CN102585277B (en) * | 2012-02-16 | 2013-04-24 | 武汉纺织大学 | Process for preparing keratin porous membrane by refrigerating |
| CN108373544A (en) * | 2018-02-06 | 2018-08-07 | 安徽工程大学 | A kind of preparation method of nanometer of feather down azelon film |
| CN108373544B (en) * | 2018-02-06 | 2021-02-02 | 安徽工程大学 | Preparation method of nano feather down protein fiber membrane |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106119330B (en) | A kind of preparation method of fish scale collagen range of hydrolysed peptides | |
| CN104031753A (en) | Detergency-improved tableware detergent composition with suspended particles | |
| CN102504327B (en) | Method for preparing cellulose sponge by utilizing sisal hemp wastes hemp bran and hemp head | |
| CN102475266A (en) | Preparation method of natural zinc-rich oyster powder | |
| CN104389216A (en) | Method for separating lignin and holocellulose from wood fiber raw materials and application thereof | |
| CN103181586A (en) | Deodorization method for pearl mussel meat | |
| CN104341535B (en) | A kind of high-valued extracting method of Enteromorpha | |
| CN101412746A (en) | Preparation of feather protein film | |
| CN103966265A (en) | Method for preparing biogas by pretreating manioc waste with NaOH through anaerobic fermentation | |
| CN103665195A (en) | Method for extracting agar from gracilaria with enzymatic method | |
| CN102627699A (en) | Extraction method for high acyl gellan gum | |
| CN108456643A (en) | A kind of preparation method of the composite bacteria agent of degrading maize bar | |
| CN102283552B (en) | Production method of artemisia herbaceous plant stem disposable hygienic chopstick | |
| CN102041204B (en) | Method for preparing tree-starch based natural biomass detergent | |
| CN103755837A (en) | Method for preparing heparin sodium by utilizing small intestines of pigs | |
| CN106753504A (en) | A kind of method that biomass liquefying produces liquid fuel | |
| CN108771151A (en) | A kind of method that medium temperature prepares sterile shrimping beam trawl dry powder | |
| CN105087660B (en) | A kind of closed cycle production method of straw ethanol-methane coproduction | |
| CN104725648A (en) | Preparation method of degradable feather protein/sodium carboxymethylcellulose composite membrane | |
| CN101092447B (en) | Fibrin enzymolysis auxiliary agent from stalk stuff of plant | |
| Liu et al. | A colorimetric total volatile base nitrogen-responsive film with antibacterial and leaching-resistant properties for accurate tracking fish quality | |
| CN101926406B (en) | Biological deodorization and discoloration method for proteins from silk-reeling silkworm chrysalises | |
| CN107384976A (en) | Directly utilize Production by Microorganism Fermentation xylitol and the complete set of equipments of arabinose | |
| CN104610577B (en) | Use the method that Exocarpium Juglandis Immaturus prepares living beings plastics | |
| CN104725649A (en) | Preparation method of in-situ cross-linked feather keratin hybrid membrane |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090422 |