CN101411611B - Optical image-forming device and method for medical diagnosis of biological tissue of human body - Google Patents

Abstract

The invention relates to an optical imaging device and an optical imaging method for medical diagnosis of human biological tissues. The optical imaging system has high imaging quality, and the minimum optical resolution is more than 151p/mm (unit: 1p/mm, line pairs per millimeter) at a modulation (namely contrast) that a modulation transfer function (MTF) is equal to 60 percent, or is more than 401p/mm at the modulation (namely contrast) that the modulation transfer function (MTF) is equal to 10 percent. The optical imaging device can provide valuable medical diagnostic information of the human biological tissues, particularly the characteristics of early pathological changes or heteroplasia and so on, and can fully obtain characteristic information of the biological tissues. The optical imaging method is an effective, quick and reliable analysis method for the medical diagnosis according to images formed by an optical imaging system, obtains accurate positions and scales of the medical diagnosis of pathological changes, and improves the performance of medical diagnostic results of the pathological changes.

Description

The optical imaging device that is used for the biological tissue of human body medical diagnosis

Technical field

The present invention relates to a kind of optical imaging device and method that is used for the biological tissue of human body medical diagnosis, specifically is a kind ofly to be used for the Optical devices of biological tissue of human body imaging and to utilize the imaging image to carry out the analytical method of medical diagnosis.

Background technology

Present traditional optical imaging device that is used for the biological tissue of human body medical diagnosis and method, main shortcoming is:

1. be summed up as requirement to the biological tissue of human body imaging, short as operating distance, optics enlargement ratio height, imaging circumstances is special to wait requirement to make traditional optical system visual field (view of field) and field depth (depth of field) design have considerable restraint.The image quality of optical imaging system is not good, minimum optics spatial resolution (optical resolution) when modulation transfer function (MTF) equals 60% modulation degree (modulation) and is contrast (contrast) less than 5lp/mm (lp/mm of unit, every millimeter line to) or when modulation transfer function (MTF) equals 10% modulation degree and is contrast less than 10lp/mm.

2. lack the valuable medical diagnostic information of biological tissue of human body, characteristics such as early lesion or abnormal development can't be provided, can not fully obtain biological tissue's characteristic information.

3. lack the effective of medical diagnosis carried out in the image analysis, fast, reliable method.

The position and the yardstick of accurate pathological changes medical diagnosis can't be obtained, pathological changes medical diagnosis result's performance can't be improved.

Summary of the invention

The objective of the invention is to overcome the shortcoming that exists in the above-mentioned prior art, propose and realize a kind of optical imaging device and method that is used for the biological tissue of human body medical diagnosis.

The present invention includes following technical characterictic and content:

The optical imaging device that is used for the biological tissue of human body medical diagnosis, it is characterized in that: comprise being used to produce the incoherent illumination light-source system of biological tissue's absorbed radiation and/or the coherent illumination light-source system of stimulated radiation, be used to accept the optimization optical wavefront phase place modulating/demodulating imaging system of corresponding biological tissue absorbed radiation and/or stimulated radiation frame, described luminous source system and imaging system are combined and are configured to have tunable light beats information state function W (f, σ, E, θ), wherein: it is the centre wavelength inverse that f represents the mid frequency of light beats, σ represents the frequency bandwidth of light beats, E represents the emittance of light beats, and θ represents the phase place of the vibrational state direction of light beats.

Biological tissue of human body is specifically drawn together epithelial cell as the epithelium tissue pocket, the pigment cell, connective tissue comprises collagen protein, mucous membrane tissue comprises hemoglobin etc., under the coherent illumination light-source system condition of incoherent illumination light-source system that produces biological tissue's absorbed radiation and/or stimulated radiation, accept the image packets that optical signature that the optimization optical wavefront phase place modulating/demodulating imaging system of corresponding biological tissue absorbed radiation and/or stimulated radiation frame obtained forms and contain different bio informations, characteristic with the corresponding biological tissue of abundant reflection information, the characteristic of biological tissue of human body valuable medical diagnostic information, particularly early lesion or abnormal development etc. can be provided.

When adopting the incoherent illumination light-source system, biological tissue of human body can produce the radiating wave optics feature of photonic absorption, and the optimization optical wavefront phase place modulating/demodulating imaging system that is used to accept biological tissue's absorbed radiation frame is obtained above-mentioned optical signature and formed biological tissue's image information.

When adopting the coherent illumination light-source system, biological tissue of human body can produce the quantum optices feature of photon stimulated radiation, and the optimization optical wavefront phase place modulating/demodulating imaging system that is used to accept biological tissue's stimulated radiation frame is obtained above-mentioned optical signature and formed biological tissue's image information.

Be used to produce biological tissue's absorbed radiation and/or stimulated radiation incoherent illumination light-source system and/or coherent illumination light-source system, can be by incoherent surface LED and/or relevant laser diode, the combination optical narrow band filter constitutes.

The light beats information state is as carrier, all information of biological organism optical feature have been expressed, the light beats message reflection of different conditions the characteristic of corresponding biological tissue information, for the valuable medical diagnostic information of biological tissue of human body more fully is provided, adopt the combination of luminous source system and imaging system to be configured to have tunable light beats information state function W (f, σ, E, θ), in fact, by tuning different light beats information state, just can produce different biological tissue's characteristic informations, early lesion or dysplastic characteristic also can further be disclosed, and potential or tangible pathological changes information can both guarantee to be obtained reliably.

Further, be used for tunable light beats information state function W (f, σ, E, θ), concrete parameter configuration is:

The mid frequency f of light beats is the centre wavelength inverse, is configured to comprise at least two frequency bands of near ultraviolet NUV and near-infrared NIR, and described near ultraviolet NUV wave-length coverage is 300-480nm, and described near-infrared NIR wave-length coverage is 680-1000nm;

The frequency bandwidth σ of light beats is configured to＜the narrowband wavelength bandwidth of 30nm;

The emittance E of light beats is configured to E=C* (Eo) ², wherein: Eo is the optics least unit area emittance of biological tissue of human body absorbed radiation and/or stimulated radiation imaging, and C is biological tissue of human body absorbed radiation and/or stimulated radiation optics conversion constant;

The phase theta of the vibrational state direction of light beats is configured to 0,45,90 degree.

Adopt optical wavefront phase place modulating/demodulating imaging system (optical wavefrontphase-modulation/demodulation imaging system), it guarantees to have field depth (the depth of field) scope of traditional optical imaging system more than 10 times under maximization luminous flux condition, simplifies the design of optical system visual field (view of field) and aberration correction simultaneously.Optimization Wave-front phase modulating/demodulating imaging system as described in Figure 1 comprises: optics entrance pupil unit, spherical lens group unit, Wave-front phase modulated optical cell, CMOS optical image sensor image-generating unit, Digital Signal Processing image demodulation recovery unit is rebuild the original digital image unit.L1, L2 are the spherical lens group, and Wave-front phase modulated optical element is as L1, the phase place pupil between the L2 battery of lens.

Definition Wave-front phase modulated optical element have odd symmetric pupil phase modulation function Φ (x, y):

$\left\{\begin{array}{c}\mathrm{\Φ}(x,y)=\underset{m=0}{\overset{M}{\mathrm{\Σ}}}\underset{n=0}{\overset{N}{\mathrm{\Σ}}}{\mathrm{\α}}_{\mathrm{mn}}{x}^m{y}^n\\ \mathrm{\Φ}(-x,-y)=-\mathrm{\Φ}(x,y)\end{array}\right.$

Wherein: M, N are exponent number, and α mn is a numeric factors.When practical application, consider numerical computations and actual requirements such as complexity of making, generally adopt exponent number less than 9 low order, as adopt 7,5,3 to be exponent number.

Wave-front phase modulating/demodulating imaging system has optics point spread function PSF (u, v; θ)=| h (u, v; θ) | ²:

$h(u,v;\mathrm{\θ})=$

$\frac{1}{\mathrm{\λf}\sqrt{A}}\∫\∫P(x,y)\exp \left\{i\right[\frac{2\mathrm{\π}}{\mathrm{\λf}}(\mathrm{ux}+\mathrm{vy})+\mathrm{\Φ}(x,y)+\mathrm{\θ}(x^2+y^2)+\mathrm{Zernike}(x,y)\left]\right\}\mathrm{dxdy}$

Wherein: P (x, y) be the pupil function of going into of optical system, as integral parameter (x, P (x when y) being included in the pupil scope, y)=1, and P in the time of in not being included in the pupil scope (x, y)=0, go into the domain of definition areal extent that is expressed as 2 dimension definite integral that pupil function also can be of equal value, the domain of definition area integral scope that promptly limits 2 dimension definite integral is the pupil scope;

θ is the diffracted wave aberration or defocuses parameter; λ is a wavelength, and f is L1, and the equivalent focal length of L2 spherical lens group, A are the pupil area of optical system, and (x y) is the Zernike aberration function of optical system to Zernike;

In fact consider that optical system has global characteristics, above-mentioned 2 dimension integrations also can employing polar coordinate integral representation of equal value.According to pupil phase modulation function Φ (x, definition y) is point spread function PSF (u, v as can be known; θ) be even symmetry.

Have modulation transfer function (MTF) and diffracted wave aberration (diffraction-aberration) in conjunction with the pupil phase modulation function Φ of the optimized Wave-front phase modulating/demodulating of space/frequency domain imaging system (x, y) satisfy essential condition A and sufficient condition B:

Wherein: diffracted wave aberration optimization degree J is by definite to give a definition:

$J={\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}\∫\∫{\left|(\∂/\∂\mathrm{\θ})\right[\mathrm{PSF}(u,v;\mathrm{\θ})\left]\right|}^2\mathrm{dudv\; d\θ}$

Or

$J=\∫\∫\left\{\mathrm{Var}\right[\mathrm{PSF}(u,v;\mathrm{\θ})\left]\right\}\mathrm{dudv}$

$=\∫\∫{\text{\∫}}_{-\mathrm{\θ0}}^{\mathrm{\θ}0}{\left|\right[\mathrm{PSF}(u,v;\mathrm{\θ})]-{\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}[\mathrm{PSF}(u,v;\mathrm{\θ})\left]\mathrm{d\θ}\right|}^2\mathrm{d\θdudv}$

$=\∫\∫{\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}{\left|\right[\mathrm{PSF}(u,v;\mathrm{\θ})\left]\right|}^2\mathrm{d\θdudv}-\∫\∫{\left|{\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}\right[\mathrm{PSF}(u,v;\mathrm{\θ})\mathrm{d\θ}\left]\right|}^2\mathrm{dudv}$

Wherein: Var is the mould variance function; [θ 0, and θ 0] specified diffracted wave aberration or defocus the parameter symmetrical range when the practical application;

According to optical theory, Wave-front phase modulating/demodulating imaging system has optical transfer function OTF (s, t simultaneously; θ) be PSF (u, v; Fourier transfer pair θ), and following inference is arranged:

$J={\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}\∫\∫{\left|(\∂/\∂\mathrm{\θ})\right[\mathrm{OTF}(s,t;\mathrm{\θ})\left]\right|}^2\mathrm{dsdtd\θ}$

Or it is corresponding

$J=\∫\∫\left\{\mathrm{Var}\right[\mathrm{OTF}(s,t;\mathrm{\θ})\left]\right\}\mathrm{dsdt}$

$=\∫\∫{\text{\∫}}_{-\mathrm{\θ0}}^{\mathrm{\θ}0}{\left|\right[\mathrm{OTF}(s,t;\mathrm{\θ})]-{\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}[\mathrm{OTF}(s,t;\mathrm{\θ})\left]\mathrm{d\θ}\right|}^2\mathrm{d\θdsdt}$

$=\∫\∫{\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}{\left|\right[\mathrm{OTF}(s,t;\mathrm{\θ})\left]\right|}^2\mathrm{d\θdsdt}-\∫\∫{\left|{\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}\right[\mathrm{OTF}(s,t;\mathrm{\θ})\mathrm{d\θ}\left]\right|}^2\mathrm{dsdt}$

Modulation transfer function (MTF) optimization degree M is by definite to give a definition:

$M={\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}\∫\∫{\left|\right[\mathrm{PSF}(u,v;\mathrm{\θ})\left]\right|}^2\mathrm{dudvd\θ}$

$={\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}\∫\∫{\left|\right[\mathrm{OTF}(s,t;\mathrm{\θ})\left]\right|}^2\mathrm{dsdtd\θ}$

$={\∫}_{-\mathrm{\θ}0}^{\mathrm{\θ}0}\∫\∫{\left|\right[\mathrm{MTF}(s,t;\mathrm{\θ})\left]\right|}^2\mathrm{dsdtd\θ}$

(x y) is satisfying under the above-mentioned sufficient and necessary condition, and Wave-front phase modulating/demodulating imaging system has modulation transfer function (MTF) and diffracted wave aberration in conjunction with space/frequency domain optimization according to above-mentioned definition and the provable pupil phase modulation function of inference Φ.

Wave-front phase modulated optical element can manufacture and design by micron-sized aspheric surface injection moulding process, can reduce cost and be easy to produce.

The image O of CMOS optical image sensor imaging (x y), recovers by the demodulation of Digital Signal Processing image, the result rebuild original digital image I (x, y).The demodulation of Digital Signal Processing image recovers specifically:

I(x，y)＝O(x，y)*h(x’，y’)＝∫∫O(x-x’，y-y’)h(x’，y’)dx’dy’

Wherein, (s t) is the modulation transfer function (MTF) function of predetermined optical system to H, and * represents 2 dimension convolution of functions integrations.H (x ', y ')=F ^-1(1/H (s, t)), i.e. H (s, the t) Fourier of falling inverse of a number conversion.

In fact, H (s, t) determine for predetermined optical system, so h (x ', y ') also determine, and the convolution yardstick of h (x ', y ') also is tight support, above-mentioned Digital Signal Processing image demodulation recovers and can express with the mathematics discrete form, can optimize the integer code by digital signal processing appts real-time implementation such as FPGA or DSP.

Fig. 2 is that the MTF of traditional optical imaging system and optimization optical wavefront phase place modulating/demodulating imaging system measures curve chart, and abscissa is spatial frequency (spatial frequency), and every millimeter line of unit is to (lp/mm), and vertical coordinate is normalization MTF data.The minimum optics spatial resolution of traditional optical imaging system (optical resolution) when modulation transfer function (MTF) equals 60% modulation degree (modulation) and is contrast (contrast) less than 5lp/mm (lp/mm of unit, every millimeter line to) or when modulation transfer function (MTF) equals 10% modulation degree and is contrast less than 10lp/mm.

The minimum optics spatial resolution (optical resolution) of optimization optical wavefront phase place modulating/demodulating imaging system when modulation transfer function (MTF) equals 60% modulation degree (modulation) and is contrast (contrast) greater than 15lp/mm (lp/mm of unit, every millimeter line to) or when modulation transfer function (MTF) equals 10% modulation degree and is contrast greater than 40lp/mm.

Further, optical imaging device uses under the complex background environment of practical application, consider the wavelength complexity, non-imaging veiling glares etc. are to the influence of image quality, the optics narrow band filter that described luminous source system and imaging system are configured to mate, described luminous source system and imaging system are configured synchronous pulsing light and imaging, and the synchronous pulsing light light-source system of employing and imaging system also can improve image quality.

The present invention is used for the analytical method of biological tissue's image medical diagnosis, and the most critical performance is effectively, and fast, reliability further, can obtain the position and the yardstick of accurate pathological changes medical diagnosis, improves pathological changes medical diagnosis result's performance simultaneously.Fig. 3 is used for the flow chart of the analytical method of biological tissue of human body image medical diagnosis for the present invention.

The invention provides a kind of analytical method that is used for the medical diagnosis of biological tissue of human body image, may further comprise the steps:

(1) absorbed radiation of definition tunable optical fluctuation information state and/or the normal reference range of stimulated radiation standard biological tissue image analytical data;

(2) utilize optical imaging device to obtain the absorbed radiation and/or the stimulated radiation biological tissue to be analyzed image I of tunable optical fluctuation information state _{F, σ, E, θ}(x, y);

(3) analytical data in the partial analysis zone of mensuration biological tissue to be analyzed image and the analytical data of the overall situation;

(4) whether the analytical data of comparison biological tissue to be analyzed image in the standard normal reference range, is the result who provides normal medical diagnosis, otherwise provides the result of unusual medical diagnosis, and the position in sign partial analysis zone is center and yardstick.

Described standard biological tissue image analytical data can be obtained by priori, provides the criterion that normal reference range is used to the medical diagnosis result.By regulating normal reference range, control medical diagnosis result's criterion sensitivity as by regulating narrower normal reference range, improves the criterion sensitivity,, vice versa.

Described biological tissue image I _{F, σ, E, θ}(x y) is illustrated in different tunable optical fluctuation information states (f, σ, E, the corresponding different images that produces under absorbed radiation θ) and/or the stimulated radiation condition.

The analytical data production method of the analytical data in the partial analysis zone of biological tissue's image and the overall situation may further comprise the steps:

(1) is defined in biological tissue's image I _{F, σ, E, θ}(x, y) the middle generation so that (xo yo) is analysis center, S=2 ⁱ(i=1,2,3...N) for analyzing the partial analysis zone of yardstick;

(2) mathematic expectaion A and the variance U in the definition partial analysis zone,

$A=1/S^2{\∫}_{\mathrm{yo}-S/2}^{\mathrm{yo}+S/2}{\∫}_{\mathrm{xo}-S/2}^{\mathrm{xo}+S/2}{I}_{f,\mathrm{\σ},E,\mathrm{\θ}}(x,y)\mathrm{dxdy}$

$u=1/S^2{\∫}_{\mathrm{yo}-S/2}^{\mathrm{yo}+S/2}{\∫}_{\mathrm{xo}-S/2}^{\mathrm{xo}+S/2}{\left[I_{f,\mathrm{\σ},E,\mathrm{\θ}}(x,y)\right]}^2\mathrm{dxdy}$

(3) in the absorbed radiation of all different tunable light beats information states of identical partial analysis region generating and/or the mathematic expectaion set A of stimulated radiation biological tissue image _{F, σ, E, θ}With variance set U _{F, σ, E, θ}

(4) definition mathematic expectaion set A _{F, σ, E, θ}With variance set U _{F, σ, E, θ}Normalization data be the analytical data in partial analysis zone.The purpose that normalization data is handled is promptly not have dependency for the image-forming condition no dependence with image, particularly when practical application, can improve the reliability and stability of analytical data in essence.

(5) biological tissue's image I is calculated in iteration (1)-(4) _{F, σ, E, θ}(x, y) in all different analysis centers and to analyze yardstick be the analytical data in partial analysis zone.

(6) add up the analytical data in all partial analysis zones in (5) to obtain the analytical data of the overall situation.

The partial analysis zone that is appreciated that above-mentioned different analysis centers and analysis yardstick also can of equal value be interpreted as that variable many analysis centers analyze the partial analysis zone of yardstick more.In fact, so partial analysis zone definitions has only mathematical model expression.The analytical data in described partial analysis zone possesses the absorbed radiation of all different tunable light beats information states and/or the most effective in essence reliable characteristic ability to express of stimulated radiation biological tissue image.

Has the accurate analytical data of measuring biological tissue's image local analyzed area by the above-mentioned steps processing, not only obtain the position and the yardstick of accurate pathological changes medical diagnosis, be analysis center (xo, yo) and analyze yardstick S, and the analytical data in all partial analysis zones of adding up is to obtain the unitary analysis data of the overall situation, to improve pathological changes medical diagnosis result's performance.

Said method can be expressed with the mathematics discrete form during practical application, and can optimize the integer code by digital signal processing appts real-time implementation such as FPGA or DSP.Further, according to the result of the medical diagnosis of biological tissue's image, the image imaging frame rate of feedback-controlled optics imaging device.If provide the result of normal medical diagnosis, can adopt low scaled image acquisition frame speed, otherwise provide the result of unusual medical diagnosis, can adopt image acquisition frame rate at high proportion, be used to analyze to obtain more image informations, can further improve pathological changes medical diagnosis result's performance about unusual biological tissue.

Sum up foregoing description, technical characterictic and content that the present invention proposes, a kind of optical imaging device and method that is used for the biological tissue of human body medical diagnosis.Optical imaging system possesses high imaging quality, minimum optics spatial resolution (optical resolution) when modulation transfer function (MTF) equals 60% modulation degree (modulation) and is contrast (contrast) greater than 15lp/mm (lp/mm of unit, every millimeter line to) or when modulation transfer function (MTF) equals 10% modulation degree and is contrast greater than 40lp/mm.The characteristic of biological tissue of human body valuable medical diagnostic information, particularly early lesion or abnormal development etc. can be provided, can fully obtain biological tissue's characteristic information.According to optical imaging system imaging image carry out medical diagnosis effectively, fast, reliable analytical method obtains the position and the yardstick of accurate pathological changes medical diagnosis, improves pathological changes medical diagnosis result's performance.

To and contrast accompanying drawing by specific embodiment below, the present invention is described in further detail.

Description of drawings

Fig. 1 is an optimization optical wavefront phase place modulating/demodulating imaging system schematic diagram of the present invention.

Fig. 2 is that the MTF of traditional optical imaging system and optimization optical wavefront phase place modulating/demodulating imaging system of the present invention measures curve chart.

Fig. 3 is used for the flow chart of the analytical method of biological tissue of human body image medical diagnosis for the present invention.

Fig. 4 is the omnidistance digestive tract of the specific embodiment of the invention 1 a biological tissue medical diagnosis sketch map.

Fig. 5 is used for the schematic diagram of the capsule optical imaging device of swallowing of omnidistance digestive tract medical diagnosis for the specific embodiment of the invention 1.

The specific embodiment

Embodiment 1

Use technical characterictic and content that the present invention describes, by being specific embodiment to the medical diagnosis of the omnidistance digestive tract of a kind of human body biological tissue, this embodiment describes a kind of capsule optical imaging device of swallowing, obtain omnidistance digestive tract biological tissue image, and the analytical method that the digestive tract biological tissue image that obtains is carried out medical diagnosis.

Fig. 4 has described the omnidistance digestive tract of specific embodiments of the invention 1 biological tissue medical diagnosis sketch map.Number in the figure 1 is an esophageal tissue, and 2 is stomach tissue, and 3 is intestinal tissue, and 4 is the capsule optical imaging device that can swallow, and 5 for radio signal receives and the image information digital signal processing appts, can be and treat that the medical examiner is equipped with oneself.In equipment 5, finish and receive the digestive tract biological tissue image that capsule optical imaging device 4 sends, the demodulation of Digital Signal Processing image recovers, the result rebuilds original digital image, reach the analytical method realization of the digestive tract biological tissue image that obtains being carried out medical diagnosis, the while is according to the analysis result feedback control capsule optical imaging device 4 of medical diagnosis.

Fig. 5 has described the schematic diagram of the capsule optical imaging device 4 that specific embodiments of the invention 1 can swallow.Number in the figure 10a, 10b are digestive tract biological tissue, 11a, and 11b is made of biocompatible material such as medical polyester for the can of the capsule optical imaging device that can swallow, has biological natures such as anti-gastric acid and protease decomposition.

Wherein the oval structure two ends, the left and right sides of shell have optical transmittance＞95%.

13a, 13b are radio signal emission, control, and circuit units such as power supply are used for image emissions with the imaging system imaging to radio signal receiving terminal 5, and known technology has the detailed description of pair relevant portion, does not do repetition at this.

14a, 14b is the optimization optical wavefront phase place modulating/demodulating imaging system that is used to accept corresponding digestive tract biological tissue's absorbed radiation and/or stimulated radiation frame, lay respectively at the two ends, the left and right sides of capsule optical imaging device 4, concrete technical characterictic is with reference to summary of the invention and Fig. 1.Further, the optimization optical wavefront phase place modulating/demodulating imaging system that is positioned at the two ends, the left and right sides of capsule optical imaging device has different optical imagery parameter configuration, as different focal lengths, can obtain the digestive tract biological tissue image under the different resolution, improve image medical diagnosis analytical performance.Minutias such as known imaging such as intestinal fine hair and wrinkle, needing minimum optics spatial resolution is 10lp/mm when modulation transfer function (MTF) equals 60% modulation degree.Optimization optical wavefront phase place modulating/demodulating imaging system (14a, minimum optics spatial resolution (optical resolution) 14b) when modulation transfer function (MTF) equals 60% modulation degree (modulation) and is contrast (contrast) greater than 15lp/mm (lp/mm of unit, every millimeter line to) or when modulation transfer function (MTF) equals 10% modulation degree and is contrast greater than 40lp/mm.So image quality is very sufficient for the medical imaging of digestive tract biological tissue and diagnosis, can disclose any biological tissue's minutia that diagnostic value is arranged.

15a, 15b, 15c, 15d are used to produce the incoherent illumination light-source system of biological tissue's absorbed radiation and/or the coherent illumination light-source system of stimulated radiation, can be by incoherent surface LED and/or relevant laser diode, the combination optical narrow band filter constitutes.

Described luminous source system (15a, 15b, 15c, 15d) (14a 14b) is combined and is configured to have tunable light beats information state function W (f, σ with imaging system, E, θ), further, be used for tunable light beats information state function W (f, σ, E, θ), concrete parameter configuration is:

The mid frequency f of light beats is the centre wavelength inverse, is configured to comprise at least two frequency bands of near ultraviolet NUV and near-infrared NIR, and described near ultraviolet wavelength NUV is configured to 340nm and 460nm, and described near-infrared wavelength NIR is configured to 680nm and 880nm;

The frequency bandwidth σ of light beats is configured to the narrowband wavelength bandwidth of 10nm;

The emittance E of light beats is configured to E=C* (Eo) ², wherein: Eo is the optics least unit area emittance of digestive tract biological tissue absorbed radiation and/or stimulated radiation imaging, and the typical range of digestive tract biological tissue is: 10mW/cm ²-100mW/cm ²

The phase theta of the vibrational state direction of light beats is configured to 0 and 90 degree.

Digestive tract biological tissue such as esophageal tissue 1, stomach tissue 2, intestinal tissue 3, be used to produce the incoherent illumination light-source system of digestive tract biological tissue absorbed radiation and/or the coherent illumination light-source system (15a of stimulated radiation, 15b, 15c, 15d), accept the optimization optical wavefront phase place modulating/demodulating imaging system (14a of corresponding digestive tract biological tissue's absorbed radiation and/or stimulated radiation frame, the characteristic information of the formed digestive tract of the optical signature of 14b) obtaining biological tissue, can provide digestive tract biological tissue valuable medical diagnostic information, particularly the characteristic of tumor early lesion or abnormal development etc.

Digestive tract biological tissue such as esophageal tissue 1, stomach tissue 2, intestinal tissue 3, adopt luminous source system (15a, 15b, 15c is 15d) with imaging system (14a, 14b) combination is configured to have tunable light beats information state function W (f, σ, E, θ), by tuning different light beats information state, just can produce different digestive tract biological tissue characteristic informations, tumor early lesion or dysplastic characteristic also can further be disclosed, and potential or tangible pathological changes information can both guarantee to be obtained reliably, as small gastrointestinal tract inflammation or ulcer, intestinal craw-craw etc.

In the specific embodiment 1, radio signal transmitter unit (13a, 13b) with the digestive tract biological tissue image 0 (x of CMOS optical image sensor imaging, y) be emitted to radio signal receiving terminal 5, finishing the demodulation of Digital Signal Processing image in image information digital signal processing appts 5 recovers, the result rebuild the original digestive tract digital picture I of biological tissue (x, y).

The demodulation of Digital Signal Processing image recovers specifically:

I(x，y)＝O(x，y)*h(x’，y’)＝∫∫O(x-x’，y-y’)h(x’，y’)dx’dy’

Wherein, (s is that (14a, MTF function 14b), * represent 2 dimension convolution of functions integrations in the predetermined optical system t) to H.

H (x ', y ')=F ^-1(1/H (s, t)), i.e. H (s, the t) Fourier of falling inverse of a number conversion.

H (s, t) for (14a of predetermined optical system, 14b) determine, so h (x ', y ') also determine, and h (x ', y ') convolution yardstick also is tight support, as use 16 rank (tap16) and just can satisfy actual requirement, above-mentioned Digital Signal Processing image demodulation recovers and can express with the mathematics discrete form, can optimize the integer code by real-time implementation in digital signal processing appts 5 such as FPGA or DSP.

The analytical method that is used for the image medical diagnosis of omnidistance digestive tract biological tissue in the specific embodiment 1 can obtain the position and the yardstick of accurate pathological changes medical diagnosis, improves the performance as a result of pathological changes medical diagnosis simultaneously.

Specific embodiment 1 is used for the method for omnidistance digestive tract biological tissue graphical analysis medical diagnosis, may further comprise the steps:

(1) absorbed radiation of definition tunable optical fluctuation information state and/or the normal reference range of stimulated radiation standard digestive tract biological tissue image analysis data;

(2) utilize the capsule optical imaging device to obtain the absorbed radiation and/or the stimulated radiation digestive tract to be analyzed biological tissue image I of tunable optical fluctuation information state _{F, σ, E, θ}(x, y);

(3) analytical data in the partial analysis zone of mensuration digestive tract to be analyzed biological tissue image and the analytical data of the overall situation;

(4) whether the analytical data of comparison digestive tract to be analyzed biological tissue image is in the standard normal reference range, be the result who provides normal medical diagnosis, otherwise provide the result of unusual medical diagnosis, and the position in sign partial analysis zone is center and yardstick.The analytical data production method of the analytical data in the partial analysis zone of digestive tract biological tissue image and the overall situation may further comprise the steps:

I (1) is defined in digestive tract biological tissue image I _{F, σ, E, θ}(x, produce in y) with (xo yo) be analysis center, S=2 (i=1,2,3...N) be the partial analysis zone of analysis yardstick;

Mathematic expectaion A and variance U in i (2) the definition partial analysis zone,

$A=1/S^2{\∫}_{\mathrm{yo}-S/2}^{\mathrm{yo}+S/2}{\∫}_{\mathrm{xo}-S/2}^{\mathrm{xo}+S/2}{I}_{f,\mathrm{\σ},E,\mathrm{\θ}}(x,y)\mathrm{dxdy}$

$u=1/S^2{\∫}_{\mathrm{yo}-S/2}^{\mathrm{yo}+S/2}{\∫}_{\mathrm{xo}-S/2}^{\mathrm{xo}+S/2}{\left[I_{f,\mathrm{\σ},E,\mathrm{\θ}}(x,y)\right]}^2\mathrm{dxdy}$

I (3) is in the absorbed radiation of identical all different light beats information states of partial analysis region generating and/or the mathematic expectaion set A of stimulated radiation digestive tract biological tissue image _{F, σ, E, θ}With variance set U _{F, σ, E, θ}

I (4) definition mathematic expectaion set A _{F, σ, E, θ}With variance set U _{F, σ, E, θ}Normalization data be the analytical data in partial analysis zone.The purpose that normalization data is handled is in order to have the image-forming condition no dependence of digestive tract biological tissue image, can to improve the reliability and stability of analytical data in essence.

(5) digestive tract biological tissue image I is calculated in iteration (1)-(4) _{F, σ, E, θ}(x, y) in all different analysis centers and to analyze yardstick be the analytical data in partial analysis zone.

(6) add up the analytical data in all partial analysis zones in (5) to obtain the analytical data of the overall situation.

Handle analytical data with accurate mensuration digestive tract biological tissue image local analyzed area by above-mentioned steps, the position that obtains the pathological changes medical diagnosis of accurate digestive tract biological tissue is analysis center (xo, yo), promptly analyze yardstick S with yardstick, also add up the analytical data in all partial analysis zones to obtain the unitary analysis data of the overall situation, improve performance as a result the medical diagnosis of digestion living according to principles for self-cultivation fabric texture pathological changes.

Said method can be expressed with the mathematics discrete form during practical application, and can optimize the integer code by digital signal processing appts 5 real-time implementation such as FPGA or DSP.Further, according to the analysis result of the medical diagnosis of digestive tract biological tissue image, the image imaging frame rate of the capsule optical imaging device 4 that feedback control can be swallowed.If provide the analysis result of normal medical diagnosis, can adopt low scaled image acquisition frame speed as 8 frame per seconds (fps), otherwise provide the analysis result of unusual medical diagnosis, can adopt at high proportion the image acquisition frame rate as 16 frame per second fps, be used to analyze to obtain more image informations, can further improve pathological changes medical diagnosis result's performance about abnormal digestion road biological tissue.

Technical characterictic and content that the present invention describes, can be identical or be equal to combination mutually in the scope of understanding, operations such as modification and increase and decrease are implemented to carry out specific embodiment, as adopt the optical imagery equivalence transformation, step replacement of equal value etc., medical imaging and diagnosis that optical imaging device that the present invention describes and method are applicable to all types of biological tissue of human body.

Claims (3)

1. the optical imaging device that is used for the biological tissue of human body medical analysis, it is characterized in that: comprise being used to produce the incoherent illumination light-source system of biological tissue's absorbed radiation and/or the coherent illumination light-source system of stimulated radiation, be used to receive the optimization optical wavefront phase place modulating/demodulating imaging system of corresponding biological tissue absorbed radiation and/or stimulated radiation frame, described luminous source system and imaging system are combined and are configured to have tunable light beats information state function W (f, σ, E, θ), wherein: it is the centre wavelength inverse that f represents the mid frequency of light beats, σ represents the frequency bandwidth of light beats, E represents the emittance of light beats, and θ represents the phase place of the vibrational state direction of light beats.

Described tunable light beats information state function W (f, σ, E, θ), concrete parameter configuration is:

The mid frequency f of light beats is the centre wavelength inverse, is configured to comprise at least two frequency bands of near ultraviolet NUV and near-infrared NIR, and described near ultraviolet NUV wave-length coverage is 300-480nm, and described near-infrared NIR wave-length coverage is 680-1000nm;

The frequency bandwidth σ of light beats is configured to＜the narrowband wavelength bandwidth of 30nm;

The emittance E of light beats is configured to E=C* (Eo) ², wherein Eo is the optics least unit area emittance of biological tissue of human body absorbed radiation and/or stimulated radiation imaging, C is biological tissue of human body absorbed radiation and/or stimulated radiation optics conversion constant;

The phase theta of the vibrational state direction of light beats is configured to 0,45,90 degree.

2. the optical imaging device that is used for the biological tissue of human body medical analysis according to claim 1 is characterized in that:

Described optimization Wave-front phase modulating/demodulating imaging system comprises: optics entrance pupil unit, spherical lens group unit, Wave-front phase modulated optical cell, CMOS optical image sensor image-generating unit, Digital Signal Processing image demodulation recovery unit is rebuild the original digital image unit;

Wherein, Wave-front phase modulated optical element is as the phase place pupil between the spherical lens group,

The demodulation of Digital Signal Processing image recovers specifically:

I(x，y)＝O(x，y)*h(x’，y’)＝∫∫O(x-x’，y-y’)h(x’，y’)dx’dy’

Wherein: O (x, y) be the image of CMOS optical image sensor imaging, (x is y) for rebuilding the original digital image result for I, H (s, t) be the modulation transfer function (MTF) of predetermined optical system, * represents 2 dimension convolution of functions integrations, h (x ', y ')=F-1 (1/H (s, t)), i.e. H (s, t) Dao Shuo inverse Fourier transform (Fourier) conversion.

3. the optical imaging device that is used for the biological tissue of human body medical analysis according to claim 1 is characterized in that:

The optics narrow band filter that described luminous source system and imaging system are configured to mate, described luminous source system and imaging system are configured synchronous pulsing light and imaging.

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