CN101402975A - Method for extracting blackberry seed oil from blackberry slag - Google Patents
Method for extracting blackberry seed oil from blackberry slag Download PDFInfo
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- CN101402975A CN101402975A CNA2008102345379A CN200810234537A CN101402975A CN 101402975 A CN101402975 A CN 101402975A CN A2008102345379 A CNA2008102345379 A CN A2008102345379A CN 200810234537 A CN200810234537 A CN 200810234537A CN 101402975 A CN101402975 A CN 101402975A
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- blackberry
- blueberry
- slag
- grease
- seed oil
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Abstract
The invention provides a production method for extracting blackberry seed oil from blackberry residues, belonging to the oil and grease processing technology field. The blackberry residues are screened by a standard sieve with 60-100 meshes after crushing, the enzymolysis is carried out by using a complex enzyme preparation of cellulase and pectinase, and the blackberry seed oil is prepared by extraction by a No.6 extraction solvent, separation, decolorization and filtration. The production method utilizes the modern biotechnology, utilizes the destruction effects of the cellulase and the pectinase to cellulose and pectin in the blackberry residues, fully releases oil and grease in blackberry seeds and adopts the combination of water phase and organic solvent extraction for effectively improving the yield of oil and grease. The production method has scientific and reasonable process design, small environmental pollution, high product quality and broad market prospect.
Description
One, technical field
The present invention is a method of extracting blackberry, blueberry seed oil in a kind of blackberry, blueberry slag, and modern biotechnology is applied to the production of blackberry, blueberry seed oil, is exclusively used in the processing of blackberry, blueberry seed oil, belongs to grease processing technology field.
Two, background technology
The present blackberry, blueberry output of China has 5.5 ten thousand tons, accounts for 11.9% of world's aggregate demand.Because factors such as developed country's raw material such as America and Europe and labor forces, turnout is just gradually reducing, in the development and less developed country, regional turnout increase year by year.In recent years, provinces such as Jiangsu, Shandong, Anhui, Sichuan, Heilungkiang and Jilin introduce a fine variety blackberry, blueberry one after another, and output increases year by year.The blackberry, blueberry slag is as the production waste of China blackberry, blueberry processing enterprise, because its acidity height, palatability is poor, and protein content is low, and contains a large amount of hard blackberry, blueberry seeds that can't digest, and is difficult to processing and utilization.Blackberry, blueberry slag major ingredient is blackberry, blueberry seed (about 85%), contain the functional grease about 13% in the blackberry, blueberry seed with health protection effect, its unsaturated fatty acids total amount and essential amino acid content are very high, to atherosclerosis, aspects such as anti-diabetic, immunomodulatory, promotion growth and intelligence growth have important effect.
Utilize blackberry, blueberry slag production blackberry, blueberry seed oil, can improve the comprehensive utilization ratio of blackberry, blueberry, enrich the blackberry, blueberry range of product, prolong the industrial chain of blackberry, blueberry, improve the rate of profit level of blackberry, blueberry industry, the healthy and rapid development that promotes the blackberry, blueberry industry is had good promoter action.
Three, summary of the invention
Technical problem the invention provides a kind of production method of extracting blackberry, blueberry seed oil from the blackberry, blueberry slag, and gained blackberry, blueberry seed oil color is limpid, balanced in nutrition, unsaturated fatty acids total amount and essential amino acid content height.
Extract greasy method in a kind of blackberry, blueberry slag of technical scheme, comprising:
1) fragmentation of blackberry, blueberry slag: raw material blackberry, blueberry slag is crossed 60～100 mesh standard sieves after fragmentation;
2) water enzymolysis: the adding mass volume ratio is 1: 1～1: 5 a tap water in the blackberry, blueberry slag, regulates pH to 4.5～6.0 with the sodium hydroxide solution of 1mol/L.Add 30～80u/g blackberry, blueberry slag Mierocrystalline cellulose, pectin compound enzyme, stop enzymolysis at 40～60 ℃ of enzymolysis 30～60min, be warming up to 90～95 ℃ of insulation 60min, the enzyme that goes out obtains suspension.
3) organic solvent extraction: add soybean extracting solvent no.6 in solvent and 1: 0.5～1: 1 ratio of raw material in suspension, extraction 40min is stirred in sealing, stops to stir, and takes out the upper strata organic solvent after leaving standstill 2h, through the reclaim under reduced pressure organic solvent, obtains crude oil.
4) grease-decolor purifies: add 1%～2% atlapulgite in crude oil, adopt steam to stir decoloration process, treat to adopt when the grease temperature is reduced to below 60 ℃ Plate Filtration to remove atlapulgite.Grease after the filtration obtains finished product blackberry, blueberry seed oil through the micro-filtrate membrane filtration of 0.35 μ m again.
Above-mentioned Mierocrystalline cellulose, pectin compound enzyme are that the polygalacturonase by the cellulase of 20000u/g and 50000u/ml forms according to 3: 1～5: 1 mixed of vigor.
Beneficial effect the present invention is directed to the blackberry, blueberry slag processing and utilization problem that produces in the present blackberry juice-processing process, adopt modern biotechnology, the method that combines by water enzymolysis and organic solvent extraction, effectively extract the grease composition of blackberry, blueberry seed in the blackberry, blueberry slag, improve the level of comprehensive utilization of China's blackberry, blueberry processing.
Major advantage of the present invention and positively effect are as follows:
1. utilize the destruction of cellulase and polygalacturonase, the grease in the blackberry, blueberry seed is fully discharged, improve the grease yield Mierocrystalline cellulose and pectin in the blackberry, blueberry slag;
2. the method that adopts water enzymolysis and organic solvent extraction to combine reduces the usage quantity of organic solvent at oil extraction, cuts down the consumption of energy, and improves product quality.
Four, embodiment
Embodiment 1
The broken blackberry, blueberry slag 100kg that crosses 60 mesh standard sieves learns from else's experience, add the 300L tap water, sodium hydroxide solution with 1mol/L is regulated pH to 5.5, add 30u/g Mierocrystalline cellulose, pectin compound enzyme (cellulase of 20000u/g and the polygalacturonase of 50000u/ml are according to 3: 1 mixed of vigor), behind 60 ± 5 ℃ of enzymolysis 50min, be warming up to 95 ℃ of insulation 60min, the enzyme that goes out obtains suspension.(product standard: GB 16629-96), extraction 40min is stirred in sealing, stops to stir, and takes out supernatant liquid after leaving standstill 2h, and the reclaim under reduced pressure organic solvent obtains crude oil to add soybean extracting solvent no.6 in solvent and 1: 0.8 ratio of raw material in suspension.Add 1% atlapulgite in crude oil, steam stirs decolouring, treats to adopt when the grease temperature is reduced to 60 ℃ plate-and-frame filter press (XAZ70/800-UB, the two whale filter company limiteds in Hangzhou) to remove atlapulgite.Grease after the filtration obtains 11.3 kilograms of blackberry, blueberry seed oil, grease yield 11.05%, extraction yield 86% through the micro-filtrate membrane filtration of 0.35 μ m again.
Embodiment 2
Get the blackberry, blueberry fresh fruit back blackberry, blueberry slag of squeezing the juice and cross the blackberry, blueberry slag 100kg of 60 mesh standard sieves through fragmentation, add the 100L tap water, sodium hydroxide solution with 1mol/L is regulated pH to 6.0, add 60u/g Mierocrystalline cellulose, pectin compound enzyme (cellulase of 20000u/g and the polygalacturonase of 50000u/ml are according to 3: 1 mixed of vigor), behind 60 ± 5 ℃ of enzymolysis 60min, be warming up to 90 ℃ of insulation 40min, the enzyme that goes out obtains suspension.Add soybean extracting solvent no.6 in solvent and 1: 0.5 ratio of raw material in suspension, extraction 40min is stirred in sealing, stops to stir, and takes out supernatant liquid after leaving standstill 2h, and the reclaim under reduced pressure organic solvent obtains crude oil.Add 2% atlapulgite in crude oil, steam stirs decolouring, treats to adopt when the grease temperature is reduced to below 60 ℃ plate-and-frame filter press (XAZ70/800-UB, the two whale filter company limiteds in Hangzhou) to remove atlapulgite.Grease after the filtration obtains 10.9 kilograms of blackberry, blueberry seed oil, grease yield 10.9%, extraction yield 83% through the micro-filtrate membrane filtration of 0.35 μ m again.
Embodiment 3
The broken blackberry, blueberry slag 100kg that crosses 100 mesh standard sieves learns from else's experience, add the 400L tap water, sodium hydroxide solution with 1mol/L is regulated pH to 5.5, add 40u/g Mierocrystalline cellulose, pectin compound enzyme (cellulase of 20000u/g and the polygalacturonase of 50000u/ml are according to 4: 1 mixed of vigor), behind 60 ± 5 ℃ of enzymolysis 50min, be warming up to 95 ℃ of insulation 60min, the enzyme that goes out obtains suspension.Add soybean extracting solvent no.6 in solvent and 1: 0.5 ratio of raw material in suspension, extraction 40min is stirred in sealing, stops to stir, and takes out supernatant liquid after leaving standstill 2h, and the reclaim under reduced pressure organic solvent obtains crude oil.Add 1% atlapulgite in crude oil, steam stirs decolouring, treats to adopt when the grease temperature is reduced to 60 ℃ plate-and-frame filter press (XAZ70/800-UB, the two whale filter company limiteds in Hangzhou) to remove atlapulgite.Grease after the filtration obtains 12.1 kilograms of blackberry, blueberry seed oil, grease yield 12.1%, extraction yield 93% through the micro-filtrate membrane filtration of 0.35 μ m again.
Claims (2)
1, extract the method for blackberry, blueberry seed oil in a kind of blackberry, blueberry slag, comprising:
1) fragmentation of blackberry, blueberry slag: raw material blackberry, blueberry slag is crossed 60～100 mesh standard sieves after fragmentation;
2) water enzymolysis: the adding mass volume ratio is 1: 1～1: 5 a tap water in the blackberry, blueberry slag, sodium hydroxide solution with 1mol/L is regulated pH to 4.5～6.0, add Mierocrystalline cellulose, pectin compound enzyme 30～80u/g blackberry, blueberry slag again, at 40～60 ℃ of enzymolysis 30～60min, stop being warming up to 90～95 ℃ of insulation 60min behind the enzymolysis, the enzyme that goes out obtains suspension;
3) organic solvent extraction: in step 2) add soybean extracting solvent no.6 in solvent and 1: 0.5～1: 1 ratio of volume feed in the suspension of Huo Deing, extraction 40min is stirred in sealing, stops to stir, and takes out upper solution after leaving standstill 2h, through the reclaim under reduced pressure organic solvent, obtain crude oil;
4) grease-decolor purifies: the atlapulgite that adds mass ratio 1%～2% in the crude oil that step 3) obtains, adopt steam to stir decoloration process, treat to adopt when the grease temperature is reduced to below 60 ℃ filter press to remove atlapulgite, the grease after the filtration obtains finished product blackberry, blueberry seed oil through the micro-filtrate membrane filtration of 0.35 μ m again.
2, according to the method for extracting blackberry, blueberry seed oil in the described a kind of blackberry, blueberry slag of claim 1, it is characterized in that: Mierocrystalline cellulose, pectin compound enzyme are formed according to 3: 1～5: 1 mixed of vigor by the cellulase of 20000u/g and the polygalacturonase of 50000u/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2008102345379A CN101402975A (en) | 2008-11-21 | 2008-11-21 | Method for extracting blackberry seed oil from blackberry slag |
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| CNA2008102345379A CN101402975A (en) | 2008-11-21 | 2008-11-21 | Method for extracting blackberry seed oil from blackberry slag |
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| CN101402975A true CN101402975A (en) | 2009-04-08 |
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| CNA2008102345379A Pending CN101402975A (en) | 2008-11-21 | 2008-11-21 | Method for extracting blackberry seed oil from blackberry slag |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101851552A (en) * | 2010-06-11 | 2010-10-06 | 淮海工学院 | Extracting method of blackberry seed oil |
| CN101921254A (en) * | 2010-08-12 | 2010-12-22 | 江苏省中国科学院植物研究所 | Method for extracting natural vitamin E from blackberry seeds |
| CN102174606A (en) * | 2011-03-07 | 2011-09-07 | 江苏省农业科学院 | Combined extraction method of blackberry seed oil and anthocyanin in blackberry juice production byproducts |
| CN102492548A (en) * | 2011-10-28 | 2012-06-13 | 东北农业大学 | Novel method for producing soybean oil based on moderate clay decoloring and high temperature treatment |
| CN103478415A (en) * | 2013-09-03 | 2014-01-01 | 汪洪涛 | Pet food and preparing process thereof |
| CN104256424A (en) * | 2014-10-14 | 2015-01-07 | 汪洪涛 | Production technology of antioxidant oral liquid taking blackberry pomace as main raw material |
| CN106987303A (en) * | 2017-04-21 | 2017-07-28 | 蚌埠市九华油脂有限公司 | A kind of method from extracting blackberry seed oil from blackberry slag |
-
2008
- 2008-11-21 CN CNA2008102345379A patent/CN101402975A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101851552A (en) * | 2010-06-11 | 2010-10-06 | 淮海工学院 | Extracting method of blackberry seed oil |
| CN101921254A (en) * | 2010-08-12 | 2010-12-22 | 江苏省中国科学院植物研究所 | Method for extracting natural vitamin E from blackberry seeds |
| CN101921254B (en) * | 2010-08-12 | 2012-05-23 | 江苏省中国科学院植物研究所 | Method for extracting natural vitamin E from blackberry seeds |
| CN102174606A (en) * | 2011-03-07 | 2011-09-07 | 江苏省农业科学院 | Combined extraction method of blackberry seed oil and anthocyanin in blackberry juice production byproducts |
| CN102174606B (en) * | 2011-03-07 | 2014-01-29 | 江苏省农业科学院 | Combined extraction method of blackberry seed oil and anthocyanin in blackberry juice production byproducts |
| CN102492548A (en) * | 2011-10-28 | 2012-06-13 | 东北农业大学 | Novel method for producing soybean oil based on moderate clay decoloring and high temperature treatment |
| CN103478415A (en) * | 2013-09-03 | 2014-01-01 | 汪洪涛 | Pet food and preparing process thereof |
| CN103478415B (en) * | 2013-09-03 | 2014-07-23 | 汪洪涛 | Pet food and preparing process thereof |
| CN104256424A (en) * | 2014-10-14 | 2015-01-07 | 汪洪涛 | Production technology of antioxidant oral liquid taking blackberry pomace as main raw material |
| CN106987303A (en) * | 2017-04-21 | 2017-07-28 | 蚌埠市九华油脂有限公司 | A kind of method from extracting blackberry seed oil from blackberry slag |
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Open date: 20090408 |