CN101401573B - Seed soaking agent for preventing bacterial wilt of ginger, preparation and uses thereof - Google Patents
Seed soaking agent for preventing bacterial wilt of ginger, preparation and uses thereof Download PDFInfo
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Abstract
The invention provides a seed soaking agent for preventing ginger bacterial wilt, as well as a preparation method and an application thereof, which belongs to the technical field of pesticide. The seed soaking agent consists of 30 to 60 percent of 80 percent sodium sorbate, 35 to 65 percent of 40 percent chlorothalonil, 0.5 to 1 percent of 80 percent sodium natrophenolate, and 1 to 5 percent of 95 percent sodium sulfite. The sodium sorbate and the chlorothalonil are mixed, ground and then passed through a 200-mesh sieve; the sodium natrophenolate and the sodium sulfite are mixed, ground and then passed through an 80-mesh sieve; and mixtures are mixed by a mixer again and then are quantificationally packaged so as to obtain the seed soaking agent. Every 100 grams of the seed soaking agent are prepared into 250 times of solution (namely 25 kilograms of solution) in order to soak 250 kilograms of ginger seeds for 20 to 30 minutes. The field control effect of the agent can reach 80 to 85 percent, and is improved by 24 to 32 percent in contrast with comparison control effect between 48 and 61 percent. The agent has the advantages of low cost which is 5 yuan per mu, sufficient raw materials, food grade, low toxicity, safety, harmlessness, simple production process, no wastewater and no waste residue.
Description
Technical field
The present invention relates to technical field of pesticide, be specifically related to a kind of seed soaking agent that prevents bacterial wilt of ginger and its production and application.
Background technology
China is the big export country of ginger, 1,500,000 mu of national ginger cultivation areas, and Yunnan is the big province of ginger outlet, ginger is one of the important exporting in Yunnan, the little yellow ginger in Luoping particularly, product has been found a good sale in 32 countries and regions such as Southeast Asia, the United Arab Emirates and America and Europe, good market prospects.But, bacterial wilt of ginger but is main in the ginger production and endangers severe diseases, each Jiang Qu all has generation in China, bacterial wilt of ginger causes the ginger production loss to account for the 15-50% (Li Xiangdong etc., the omnidistance control technology research of Luoping ginger disease pest and weed and application demonstration, " Yunnan agricultural science and technology " 04 phase in 2006) of ginger gross yield every year, some areas harm is serious, underproduction rate reaches 50%, and part field piece even cause the product that goes out badly influences peasant's productive life and economic well-being of workers and staff.All adopt the vegetative propagation of ginger piece in the ginger production, germ directly or is alternately propagated by carry disease germs stem tuber and soil water flow easily, cause that disease breaks out, bacterial wilt of ginger pathogenic bacteria source mainly is the blue or green withered false monospore Bacillus (PseudomonasSolanacearum) of bacterium, for kind, soil, water passes bacterial disease, not only infect ginger, also endanger solanaceous crops such as tomato, capsicum.Long-distance communications are the seed that carries disease germs, and the commodity vegetables allocation and transportation of carrying disease germs are propagated.2007, bacterial wilt of ginger Yunnan Province's onset area reach 20 surplus ten thousand mu, the general loss 15%～30% of morbidity field piece, some areas harm is serious, underproduction rate reaches 50%, part field piece causes the product that goes out.
Though the study on prevention of bacterial wilt of ginger has obtained some initial achievements, a lot of on the market bactericide can be prevented and treated bacterial wilt of ginger, and certain effect is arranged.In the laboratory germ of separating is carried out pharmacy test certain effect is also arranged, but still do not break through in production practices and application facet, disease still spreads rapidly, and the harm of nearly 3 years China's bacterial wilt of ginger causes damage and reaches 20% (the bacterial wilt of ginger germ also has new development and change).The technology of control bacterial wilt of ginger has seed selection, crop rotation and chemical control in the prior art.Seed selection exists to infect in the storage and carries disease germs; Crop rotation exists the restriction in soil; Effect of chemical control is not good or the like.As the control of loosing of withering of 20% blue or green withered spirit, green grass or young crops certain effect and control action are arranged to the ginger bacterial wilt of ginger; Dispenser in time is 2～3 times at the initial stage of a disease, can suppress its spread in china of bacterial wilt of ginger.But consumption is big, the cost height.Therefore, handle from the ginger kind, developing new effective seed soaking agent is pressing on the ginger kind is handled.How preparing the seed soaking agent with good prevention performance, is that a land for growing field crops anxious to be solved, this area produces the technical barrier in early stage.In view of the above, the inventor went through 5 years, had done the test of pesticide effectiveness of the domestic and international seed soaking agent of kind surplus in the of 20.Be intended to invent a kind of seed soaking agent of killing the germ of carrying on ginger kind surface, the prevention seed-borne fungi is propagated.
Summary of the invention
The objective of the invention is to overcome in the prior art land for growing field crops effect of chemical control only in 20-61% relatively poor reality, and a kind of remarkably productive and economic benefit of bacterial wilt of ginger, seed soaking agent profitable on ecology of preventing is provided.
Two of purpose of the present invention provides the preparation method of seed soaking agent of the present invention.
Three of purpose of the present invention provides the application of seed soaking agent of the present invention, obtains correct use to guarantee the present invention.
Principle of the present invention is: at bacterial wilt of ginger is to be main and the compound basis of infecting harm of fungi with the bacterium, utilizes the effect to bacterium of sodium sorbate, sodium sulphite, and tpn is to the effect of fungi, and the synergistic effect of compound sodium nitrophenolate is composited.Utilization of the present invention can be killed the bactericidal agent sodium sorbate, tpn of the bacterium of carrying on ginger kind surface and fungi and directed research and development, thereby of the present invention with strong points.
Beneficial effect of the present invention:
1, show that but the present invention's specific aim kills bacterium and the fungi that carry on ginger kind surface.Overcome in single agent and the prior art other effects preferably seed soaking agent only to bacterium or the resultful unicity of fungi.Resultant effect and advance that the present invention gives prominence to have been embodied.The present invention compared with the control, the present invention reaches 56%-92% to the control efficiency of the bacterium of bacterial wilt of ginger, the control efficiency to the fungi of bacterial wilt of ginger reaches 47%-87% simultaneously, and the contrast effect of chemical control is single.
2, field demonstration preventive effect of the present invention reaches 80%-85%, and the demonstration preventive effect of comparative examples reaches 48%-61%, and the field demonstration preventive effect of comparison photograph improves 24%-32%.
3, with 250 times of liquid of seed soaking agent of the present invention, cost per mu is low, only is 5 yuan, and every mu of ginger kind is retrieved economic losses 200 yuan by using the present invention.
4, seed soaking agent of the present invention has very strong permeability, adhesivity, adsorptivity and quick-acting and lasting effect.Can efficiently kill ginger kind surface bacteria and fungi pathogenic bacteria apace.
5, raw material abundance of the present invention, production technology is simple, and energy consumption is low, and small investment is easy to use, is easily grasped by the same domain technical staff.
6, raw material sodium sorbate, sodium sulphite are food-grade in the product of the present invention, and tpn low toxicity, product low toxicity are harmless, have the characteristics of efficient, low toxicity, economy, safety.
7, there are not waste water, waste residue, waste gas generation, energy-conservation and environmental protection in the production of the present invention.
The present invention seeks to be achieved by the following technical programs:
One, a kind of seed soaking agent that prevents bacterial wilt of ginger of the present invention, form by following component and percentage by weight:
Sodium sorbate 30%-60%;
Tpn 35%-65%;
Compound sodium nitrophenolate 0.5%-1%;
Sodium sulphite 1%-5%.
The effective component content of above-mentioned raw materials is 80% sodium sorbate, 40% tpn, 80% compound sodium nitrophenolate, 95% sodium sulphite, and its raw material is industrial products or product for agriculture.
The raw material that below respectively prevents the seed soaking agent of raw materials used and above-mentioned prevention bacterial wilt of ginger in the seed soaking agent prescription of bacterial wilt of ginger to fill a prescription is identical.
According to above-mentioned seed soaking agent, the seed soaking agent of another prevention bacterial wilt of ginger of the present invention, form by following component and percentage by weight:
Sodium sorbate 43%-54%;
Tpn 40%-55%;
Compound sodium nitrophenolate 0.5%-1%;
Sodium sulphite 1%-5%.
According to above-mentioned seed soaking agent, the seed soaking agent of another prevention bacterial wilt of ginger of the present invention, form by following component and percentage by weight:
Sodium sorbate 50%;
Tpn 45%;
Compound sodium nitrophenolate 1%;
Sodium sulphite 4%.
Each constituent content percentage sum equals 100% in the seed soaking agent prescription of above-mentioned each prevention bacterial wilt of ginger.
Two, the preparation method of the seed soaking agent of the prevention bacterial wilt of ginger of the invention described above, carry out according to the following steps:
1, in the ratio of the percentage by weight of seed soaking agent of the present invention, raw material sodium sorbate, tpn are weighed respectively, mix the back and pulverize by cracker, through pulverizing by after 200 sieve meshes, it is standby to make standby material A.
2, in the ratio of the percentage by weight of seed soaking agent of the present invention, compound sodium nitrophenolate, sodium sulphite are weighed respectively, mix the back and pulverize by cracker, through pulverizing by after 80 sieve meshes, it is standby to make standby material B.
3, add standby material A and standby material B simultaneously in mixer, after 20-25 minute, quantitative package promptly obtains seed soaking agent of the present invention through the mixer mixing.This product is a wetting powder.
Three, the seed soaking agent of the invention described above is in the application of prevention bacterial wilt of ginger
Be made into 250 times of liquid with 0.1 kilogram of seed soaking agent and soak 250 kilograms of ginger kinds, soaked seed 20-30 minute.
Embodiment
Below in conjunction with embodiment the present invention is further described.Embodiment 1-5 uses embodiments of the invention, and embodiment 6-10 is contrast, and embodiment 11 is that control in field is used.
Embodiment 1
1, the present invention prevents the seed soaking agent of bacterial wilt of ginger to be made up of following component and percentage by weight, and preparation 10kg:
Sodium sorbate 50%, 5.0kg;
Tpn 45%, 4.5kg;
Compound sodium nitrophenolate 1%, 0.1kg;
Sodium sulphite 4%, 0.4kg.
The effective component content of above-mentioned raw materials is 80% sodium sorbate, 40% tpn, 80% compound sodium nitrophenolate, 95% sodium sulphite, and its raw material is industrial products or product for agriculture.
2, the preparation method of above-mentioned seed soaking agent
(1) take by weighing sodium sorbate 5.0kg, tpn 4.5kg respectively with table scale (when consumption was big, also available other claimed), mix the back by airslide disintegrating mill, through pulverizing by after 200 sieve meshes, it is standby to make standby material A.
(2), with balance respectively (when consumption was big, also available table scale or other claimed) claim compound sodium nitrophenolate 0.1kg, sodium sulphite 0.4kg, mix the back and pulverize by cracker, through pulverizing by after 80 sieve meshes, it is standby to make standby material B.
(3), in the spiral cone-type mixer of both arms, add standby material A and standby material B simultaneously, after 20-25 minute, quantitative package (every bag of Packing Unit 50g) promptly obtains seed soaking agent of the present invention through the spiral cone-type mixer mixing of both arms, this product is a wetting powder.
3, the application of above-mentioned seed soaking agent
This test: make test material with the little yellow ginger in Luoping, every processing repeats for three times with ginger kind 20kg, every processing, altogether 60kg ginger kind.
(1) seed soaking preventive effect test
With above-mentioned seed soaking agent respectively with different consumptions and corresponding preparation times liquid (water preparation), after the ginger kind soaked seed 20 minutes, getting ginger kind epidermis is carried out the back respectively on bacteria culture media and fungi culture medium cultivated 100 hours, remake the microscopy preventive effect, the seed soaking agent of its different amounts and corresponding preparation times liquid and preventive effect see Table 1.
Above-mentioned bacteria culture media and fungi culture medium are formulated as follows according to a conventional method:
Bacteria culture media is Ralstonia solanacearum medium (Tm): Peptone (peptone) 10g; Casein (casein hydrolysate product) 1g; Glucose (glucose) 5g; Ddhro (pure water) 1000ml; Agar (agar) 15g; PH7.0.
Fungi culture medium is the potato sugar agar medium: potato is cleaned peeling, gets 200 grams and be cut into small pieces, add 1000 milliliters in water, boil half an hour after, supply moisture.In filtrate, add 10 gram agar, boil dissolving back sugaring 20 grams, supply moisture, packing, sterilization, standby.
Table 1 seed soaking test preventive effect relatively
(2) seed soaking time of seed soaking agent test
Be made into 250 times of liquid by 0.1 kilogram of above-mentioned seed soaking agent consumption, the ginger kind was soaked seed respectively 5 minutes; 10 minutes; 15 minutes; 20 minutes; 30 minutes; 40 minutes; 50 minutes; After 60 minutes; Ginger kind epidermis is carried out the back respectively cultivated 100 hours on bacteria culture media and fungi culture medium, remake the microscopy preventive effect, its preventive effect sees Table 2.The prescription of described bacteria culture media and fungi culture medium is ditto described.
The seed soaking time test effect of table 2 embodiment 1 seed soaking agent relatively
Table 2 shows, soaks seed 20-30 minute, and preventive effect is good, reaches 82%.
Seed soaking time is in the time of 40 minutes, and is not strong in the operability of production application because seed soaking time is longer though preventive effect can reach 83%, therefore do not advocate with seed soaking 40 minutes.Soak seed 50-60 minute the time, preventive effect drops to 64-57%, is because seed soaking time is longer, and it is more, perishable that the meat stem tuber of ginger kind can absorb moisture content, and effect is descended.
(3) economic benefit is calculated:
The wholesale market price lattice of seed soaking agent of the present invention are by 50 yuan of/kilogram calculating, then, with every mu be example with seed soaking agent 0.1 kilogram (kilogram), every mu with seed soaking agent cost of the present invention=0.1 kilogram seed soaking agent * 50 yuan/kilogram=5 yuan.Table 1 has been listed every mu of different seed soaking agent consumption, calculates every mu of drug cost by aforementioned same procedure.And compare with 20% blue or green withered spirit, being made into 250 times of liquid seed soaking, every mu of drug cost needs 8.0 yuan.
Reaching 80% with seed soaking agent seed soaking preventive effect of the present invention calculates, every mu retrieve the loss that the ginger disease causes after, ginger volume increase 120 kg/mu (calculating) according to field trial mensuration volume variance, the ginger unit price is by 1.7 yuan of/kilogram calculating of wholesale market price lattice, increase income: 200 yuan/mu of 204 yuan of/mu ≈ (1.7 yuan/kilogram * 120 kg/mu=204 yuan), promptly every mu of ginger kind can be retrieved economic losses 200 yuan by using the present invention.
The preventive effect of comprehensive above-mentioned test, drug cost, can draw: be made into 250 times of liquid (i.e. 25 kilograms of solution) with 0.1 kilogram of seed soaking agent and soak 250 kilograms of ginger kinds, soaked seed 20-30 minute, preventive effect and economic benefit are good, preventive effect reaches 82%, 5 yuan/mu of drug costs.
Following examples 2-5 except that the constituent content difference of following seed soaking agent, raw materials used industrial products or the product for agriculture of being, the active ingredient of its raw material is all identical with embodiment 1 with implementation method, repeats no more, its preventive effect sees table 3 for details.
Embodiment 2
The present invention prevents the seed soaking agent of bacterial wilt of ginger to be made up of following component and percentage by weight: and prepare 10kg:
Sodium sorbate 30%, 3.0kg;
Tpn 65%, 6.5kg;
Compound sodium nitrophenolate 1%, 0.1kg;
Sodium sulphite 4%, 0.4kg.
Embodiment 3
The present invention prevents the seed soaking agent of bacterial wilt of ginger to be made up of following component and percentage by weight, and preparation 10kg:
Sodium sorbate 43%, 4.3kg;
Tpn 55%, 5.5kg;
Compound sodium nitrophenolate 1%, 0.1kg;
Sodium sulphite 1%, 0.1kg.
Embodiment 4
The present invention prevents the seed soaking agent of bacterial wilt of ginger to be made up of following component and percentage by weight, and preparation 10kg:
Sodium sorbate 54%, 5.4kg;
Tpn 40%, 4.0kg;
Compound sodium nitrophenolate 1% 0.1kg;
Sodium sulphite 5% 0.5kg.
Embodiment 5
The present invention prevents the seed soaking agent of bacterial wilt of ginger to be made up of following component and percentage by weight, and preparation 10kg:
Sodium sorbate 60%, 6.0kg;
Tpn 35%, 3.5kg;
Compound sodium nitrophenolate 0.5% 0.05kg;
Sodium sulphite 4.5% 0.45kg
Embodiment 6-10: check experiment
Use 80% sodium sorbate (single agent), 40% tpn (single agent), 50% carbendazim (single agent), 77% can kill to such an extent that (single agent), 20% blue or green withered spirit (single agent) compare respectively, in these five kinds of contrasts, comparing with single agent with 80% sodium sorbate list agent and 40% tpn is that explanation creativeness of the present invention is to be to be mainly effectively to fill a prescription with the compound mechanism that infects harm of fungi with the bacterium at bacterial wilt of ginger; Select for use 50% carbendazim (single agent), 77% can kill (single agent), 20% blue or green withered spirit (single agent) to compare be can kill to such an extent that (single agent), 20% blue or green withered spirit are effect medicaments preferably in the control bacterial wilt of ginger in the prior art because of 50% carbendazim (single agent), 77%.
The seed soaking test measure of above-mentioned five kinds of contrasts sees Table 3, the method that the seed soaking agent of embodiment 6-7 is made into 250 times of liquid is identical with embodiment 1, embodiment 8-10 is made into the operation instructions preparation of the method for 250 times of liquid by each medicament, and five kinds of check experiments were soaked seed respectively 20 minutes to the ginger kind; Each handles ginger kind 20kg, three repetitions.Behind the airing, get ginger kind epidermis and carry out back cultivation 100 hours respectively on bacteria culture media and fungi culture medium, remake the microscopy preventive effect, its preventive effect sees Table 3.Described bacteria culture media is that the prescription of Ralstonia solanacearum medium (Tm) is ditto described, and described fungi culture medium is that the prescription of potato sugar agar medium is ditto described.
The test brief summary: laboratory results fully shows the present invention compared with the control, the present invention reaches 56%-92% to the control efficiency of the bacterium of bacterial wilt of ginger, control efficiency to the fungi of bacterial wilt of ginger reaches 47%-87%, and contrast is single to the control efficiency of bacterium and fungi, but shows that the present invention's specific aim kills bacterium and the fungi that carry on ginger kind surface.Overcome in single agent and the prior art other effects preferably seed soaking agent only to bacterium or the resultful unicity of fungi.Resultant effect and advance that the present invention gives prominence to have been embodied.
Table 3 the present invention prevents the seed soaking agent and contrast preventive effect statistical form of bacterial wilt of ginger
Annotate: 1, seed soaking time unit is minute in the table.
2, handling ginger kind consumption in the table is that 20kg/ handles, and every processing repeats for three times, and the shared ginger of every processing kind unit is a kilogram.
Embodiment 11 (field demonstration)
5: 500 mu of tests that arranged in 2006～2007 years the Yunnan Prov Academy Of Agricultural Sciences, Agricultural Research Institute Of Environmental Resources, 3: 1000 mu of demonstrations of arrangement in 2008.Test site: academy of agricultural sciences, Yunnan Province agricultural environment The Study on Resources institute and Luoping County, Yunnan Province.
Field demonstration is made into 250 times of liquid as stated above with the seed soaking agent of embodiment 1-10, soaks 250 kilograms of ginger kinds, soaks seed 250 kilograms of every mu of ginger kind consumptions 20 minutes.Select peasant household from storing up the ginger kind, ginger Zhong Chu Gao, seed selection, seed soaking is fished for and is dried the back and just can sow after removing kind, and cooperates other farming measure to demonstrate, and other farming measure such as crop rotation, disease pest and weed are prevented and treated identical with the conventional cultivation technical measures, repeat no more.Field demonstration preventive effect of the present invention reaches 80%-85%, and the demonstration preventive effect of comparative examples reaches 48%-61%, and the field demonstration preventive effect of comparison photograph improves 24%-32%, sees Table 4.
Experiment and demonstration is the result fully show, the present invention is applicable to large scale application fully.Seed selection after the seed soaking, and cooperates culture techniques such as conventional crop rotation, disease pest and weed control, just can obtain control efficiency efficiently.
Table 4 field demonstration preventive effect of the present invention
| Embodiment | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Demonstration preventive effect (%) | 80 | 83 | 81 | 80 | 85 | 53 | 48 | 48 | 55 | 61 |
Claims (6)
1. a seed soaking agent that prevents bacterial wilt of ginger is characterized in that, forms by following component and percentage by weight:
Sodium sorbate 30%-60%;
Tpn 35%-65%;
Compound sodium nitrophenolate 0. 5%-1%;
Sodium sulphite 1%-5%;
The effective component content of above-mentioned raw materials is 80% sodium sorbate, 40% tpn, 80% compound sodium nitrophenolate, 95% sodium sulphite, and its raw material is industrial products or product for agriculture.
2. seed soaking agent according to claim 1 is characterized in that, forms by following component and percentage by weight:
Sodium sorbate 43%-54%;
Tpn 40%-55%;
Compound sodium nitrophenolate 0. 5%-1%;
Sodium sulphite 1%-5%.
3. seed soaking agent according to claim 2 is characterized in that, forms by following component and percentage by weight:
Sodium sorbate 50%;
Tpn 45%;
Compound sodium nitrophenolate 1%;
Sodium sulphite 4%.
4. the preparation method of claim 1 or claim 2 or the described seed soaking agent of claim 3 is characterized in that, carries out according to the following steps:
(1) in the ratio of the percentage by weight of seed soaking agent of the present invention, raw material sodium sorbate, tpn are weighed respectively, mix the back and pulverize by cracker, through pulverizing by after 200 sieve meshes, it is standby to make standby material A;
(2) in the ratio of the percentage by weight of seed soaking agent of the present invention, compound sodium nitrophenolate, sodium sulphite are weighed respectively, mix the back and pulverize by cracker, through pulverizing by after 80 sieve meshes, it is standby to make standby material B;
(3) add standby material A and standby material B simultaneously in mixer, after 20-25 minute, quantitative package promptly obtains seed soaking agent of the present invention through the mixer mixing.
5. claim 1 or claim 2 or the described seed soaking agent of claim 3 are in the application of prevention bacterial wilt of ginger.
6. seed soaking agent according to claim 5 is characterized in that: be made into 250 times of liquid with 0.1 kilogram of seed soaking agent and soak 250 kilograms of ginger kinds, soaked seed 20-30 minute in the application of prevention bacterial wilt of ginger.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102335502A CN101401573B (en) | 2008-11-10 | 2008-11-10 | Seed soaking agent for preventing bacterial wilt of ginger, preparation and uses thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008102335502A CN101401573B (en) | 2008-11-10 | 2008-11-10 | Seed soaking agent for preventing bacterial wilt of ginger, preparation and uses thereof |
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| CN101401573B true CN101401573B (en) | 2011-09-28 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102001881A (en) * | 2010-10-09 | 2011-04-06 | 徐坤 | Preparation for preventing and curing ginger bacterial wilt |
| KR20200104359A (en) * | 2017-12-24 | 2020-09-03 | 오에이티 아그리오 가부시키가이샤 | Synergistic fungicide composition containing nitrophenol compound and fungicidal active compound |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079609A (en) * | 1993-03-16 | 1993-12-22 | 李俊明 | Ginger treasured and production method thereof |
| WO2006065985A2 (en) * | 2004-12-16 | 2006-06-22 | University Of Florida Research Foundation, Inc. | Materials and methods for the control of bacterial wilt in plants |
| CN1813540A (en) * | 2005-02-06 | 2006-08-09 | 赵玉发 | Medicine for preventing and treating ginger disease |
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2008
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079609A (en) * | 1993-03-16 | 1993-12-22 | 李俊明 | Ginger treasured and production method thereof |
| WO2006065985A2 (en) * | 2004-12-16 | 2006-06-22 | University Of Florida Research Foundation, Inc. | Materials and methods for the control of bacterial wilt in plants |
| CN1813540A (en) * | 2005-02-06 | 2006-08-09 | 赵玉发 | Medicine for preventing and treating ginger disease |
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