CN101398436B - Rotating micro-example auto-introducing device - Google Patents
Rotating micro-example auto-introducing device Download PDFInfo
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- CN101398436B CN101398436B CN2008100134392A CN200810013439A CN101398436B CN 101398436 B CN101398436 B CN 101398436B CN 2008100134392 A CN2008100134392 A CN 2008100134392A CN 200810013439 A CN200810013439 A CN 200810013439A CN 101398436 B CN101398436 B CN 101398436B
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Abstract
The invention relates to a rotary type micro-sample automatic leading-in device which comprises an integral sample tray, a capillary sampling probe, a step motor, a high-voltage power supply, an electrode, and a liquid storage pipe and is characterized in that the integral sample tray takes a shape of gear, specifically, liquid storage pools are evenly distributed on the outer edge of the circular tray body, and each of the liquid storage pools is opened with a hole; the outer wall of the sample inlet end of the capillary sampling probe is plated with a metal layer or a plurality of metal layers and the sample inlet end can be inserted into the hole of a liquid storage pool of the integral sample tray. When the integral sample tray rotates, the probe does not contact the body of the integral sample tray. The invention can realize automatic sample introduction and change without using any pump or valve, with the time of sample change being less than 0.2s and the rate of consumption of the sample solution being nL grade, thus the automatic leading-in device can be adopted as a high-throughput sample leading-in device for relevant analytical instruments.
Description
Technical field
The invention belongs to the microfluidic analysis technical field, particularly a kind of rotating micro-example auto-introducing device.
Background technology
Microfluidic analysis is the field, analytical chemistry forward position that grows up the twentieth century initial stage nineties; It is the basis with analytical chemistry; With micro electronmechanical process technology is support; Target is to be integrated in whole breadboard function on the microchip, makes up chip lab, realizes whole microminiaturization, robotization, the integrated and portability of various analytic systems.Micro flow control chip capillary electrophoresis is in one of microfluidic analysis field with fastest developing speed, and it has advantages such as separating fast, be prone to operation repetitive, robotization, integrated, low cost.Yet for the microfluidic analysis system of high flux, robotization, it is very important that suitable sample is introduced interface, and this also is one of bottleneck problem of restriction microflow control technique development.Require sample to introduce on the one hand and keep continuous sample introduction and not interrupt analysis process; Because microfluidic system is controlled sample size few (skin rise to receive upgrading), should study and how in the process of varying, reduce sample loss on the other hand, bring into play the advantage of microfluidic analysis system.Adopt maximum fixedly sample introducing methods of liquid storage tank formula that is based in the microcurrent controlled capillary tube electrophoresis system; This sample injection method usually is designed to cruciform [Bruin J M G.Recentdevelopments in electrokinetically driven analysis on microfabricated devices with the microchannel; Electrophoresis, 2000,21:3931-3951.]; Wherein a passage is as split tunnel, and another passage is used for sample to be introduced.The two ends of two passes are liquid storage tank, and wherein two liquid storage tanks are respectively applied for stored sample solution and damping fluid, and two other is as waste liquid pool.The variation of current potential can be handled liquid flow on the control liquid storage tank, thereby realizes the introducing and the electrophoretic separation of sample.This device is processed easily, and is simple to operate, varies but belong to batch (-type), usually varies and can only carry out by hand, often needs the operation of tens steps, not only loaded down with trivial details, and influenced analysis throughput.Varying efficient not high is one of the problem of restriction microfluidic analysis technology practical application, makes the fireballing main advantage of chip analysis be difficult to fully played.Therefore continuous sample is introduced technology has become one of problem of microfluidic analysis Technology Need emphasis solution.
Flow cell formula sample drawing-in system is to be used to address the above problem one of method [Fang Q, Wang F R, Wang S L; Liu SS, Xu S K, Fang Z L.Sequential injection sample introduction microfluidic-chip based capillaryelectrophoresis system; Anal.Chim.Acta; 1999,390:27-37.], it mainly is to be employed in the sample that processing is connected with the microchannel on the chip to introduce passage; The continuous multiple sample solution of introducing in chip is then at enterprising appearance and the lock out operation of advancing of chip.The driving force that circulation sample drawing-in system often adopts peristaltic pump, syringe pump or vacuum pump etc. to change as sample.But the each sample introduction consumption of flow type sample drawing-in system sample size is bigger, is generally tens microlitres to several hectolambdas [Fang Q, Xu G M; Fang Z L.A high-throughput continuous sample introduction interface for microfluidic chip-basedcapillary electrophoresis systems [J], Anal.Chem., 2002; 74:1223-1231. Xu Zhang Run, Wang Shili, Fan Xiaofeng; Wang Furen, Fang Zhaolun, but the development of small-sized continuous sample introduction micro-fluidic chip analyzer; Analytical chemistry, 2003,31 (12): 1527-1530.Wang S L; Fan X F; Xu Z R, Fang Z L.A simple microfluidic system for efficient capillary electrophoreticseparation and sensitive fluorimetric detection of DNA fragments using light-emitting diode andliquid-core waveguide techniques, Electrophoresis; 2005,26:3602-3608.].The characteristic of sample probe style article drawing-in system is not have special fixing or circulation sample cell on the chip; And adopt the sample probe that directly links to each other with the chip analysis passage, through being inserted, sample probe accomplishes sample introducing [Smith E M, Xu HW in the sample receiver; Ewing A G.DNAseparations in microfabricated devices with automated capillary sample introduction; Electrophoresis, 2001,22:363-370.Chen G; Wang J.Fast and simple sample introduction forcapillary electrophoresis Microsystems; Analyst, 2004,129:507-511.].But the coupon array that cracks of sample probe and translation is combined, can realize low consumption, the introducing of high flux sample [He Q H, Fang Q, Du W B; Huang Y z, Fang Z LAnalyst, 2005; 130:1052-1057.He Q H, Fang Q, Du W B; Fang Z L.Electrophoresis, 2007,28:2912-2919.].Coupon is difficult to guarantee the consistance of its size and dimension but this device home built cracks, and the sample probe reliability of binding electric pole type is relatively poor, has limited its actual application ability.In addition, one-dimensional linear coupon array takes up room bigger, and needs the array length of twice during the translation sample introduction, is unfavorable for the miniaturization of device.Therefore, develop a kind of robotization sample introduction device with practical value,, promote the development of microfluidic analysis technology, and can be this technological industrialization and pave the way the bottleneck problem that solves microflow control technique development to a certain extent.
Summary of the invention
The present invention is directed to the problem that existing sample introducing technology exists, a kind of rotating micro-example auto-introducing device is provided.
Rotating micro-example auto-introducing device of the present invention comprises integrated sample disc, kapillary sample probe, stepper motor, high-voltage power supply, electrode, liquid storage pipe.
Integrated sample disc of the present invention is a gear shape; Bossing promptly evenly distributes in the circular disk body outer; The upper surface of each bossing has a groove, bossing and its with groove constitute liquid storage tank, each liquid storage tank is evenly distributed on the integrated sample disc; Each liquid storage tank exterior bottom has a hole, and each hole is communicated with the inner and integrated sample disc of the liquid storage tank outside; Integrated sample disc is fixed on the axle of stepper motor, with the axle concentric of stepper motor.
Kapillary sample probe material of the present invention is fused quartz kapillary or high molecular polymer kapillary; Capillary inner diameter is 1～1000 μ m; High molecular polymers such as fused quartz outer wall coating polyimide capillaceous or amorphous teflon wherein are in case it fractures; The outer wall of the sample inlet end of kapillary sample probe is through sputter or plating mode; Form one or more layers coat of metal; Play electric action, coat of metal gross thickness is 0.1～200 micron, and wherein outermost layer coating can adopt antiseptic power materials such as stainless steel, platinum or gold preferably.
High-voltage power supply of the present invention is a DC high-voltage power supply; A power supply wherein utmost point passes through the lead connection electrode; The sample export end of this electrode and kapillary sample probe is inserted in the liquid storage pipe, and another utmost point of power supply is wired on the coat of metal of sample inlet end of kapillary sample probe.The electrode connection mode of power supply is according to by electrically charged switching of analytic sample.
The sample inlet end of kapillary sample probe can be inserted in the hole of liquid storage tank bottom of integrated sample disc; And can be under the constant situation in holding position; Rotation through integrated sample disc is inserted into respectively in the hole of each liquid storage tank, and does not contact the disk body of integrated sample disc.
Integrated sample disc of the present invention is formed by machining or mold injection, and diameter is 1～100cm, and thickness is 0.1～10cm, and the side of disk body and liquid storage tank inwall minor increment are not more than 2mm; As required, the quantity of liquid storage tank can the variation from 3 to 1000, evenly distributes at the sample disc edge; For preventing solution evaporation in the liquid storage tank, can on liquid storage tank, add a cover plate, cover plate can be processed by materials such as silicon rubber.
Liquid storage tank of the present invention inside is cylindrical; Diameter 0.5～5mm; The degree of depth 2～50mm, the wide 0.5～5mm of the hole of its lower outside does not contact the kapillary sample probe when integrated sample disc is rotated; And the kapillary sample probe can be inserted into the fluid sample that places in the liquid storage tank, and the coat of metal need touch fluid sample simultaneously.
The method of application of rotating micro-example auto-introducing device of the present invention is: integrated sample disc is rotated with stepper motor; And at the back stop certain hour that turns an angle; The kapillary sample probe contacts with sample solution in the hole during stop; Apply certain DC voltage through high-voltage power supply at kapillary sample probe two ends, sample gets in the kapillary sample probe under electric field action, promptly accomplishes the sample introduction process.
Kapillary sample probe among the present invention is as the capillary electrophoresis separation passage; In the kapillary sample probe; Adopt the sample of tagged under effect of electric field, to separate, utilize the liquid core waveguide phenomenon, through laser vertical irradiation kapillary sample probe; The exciting light that fluorescent material produces axially conducts along the kapillary sample probe, and is transmitted to the pick-up unit analysis result at the sample export end of kapillary sample probe.
According to the present invention, stepper motor is used to drive integrated sample disc and rotates, and its rotating speed should not surpass 10rad/s, in case the solution in the liquid storage tank leaks out from hole under centrifugal action below 10rad/s; Stepper motor can stop certain hour in the back that turns an angle by microcomputer or Single-chip Controlling.
According to the present invention; Each liquid storage tank interval of described integrated sample disc adds buffer solution and different sample solutions; When the integrated plastic sample disc is rotated under stepper motor drives; The kapillary sample probe alternately contacts with different sample solutions with buffer solution, under effect of electric field, accomplishes the process of varying; Rotate continuously sample disc, can realize continuous auto injection and vary, need be by any pump and valve, and the time of varying less than 0.2s, the sample solution consumption is the nL level.
The present invention is through setting up a kind of dependable performance, the automatic drawing-in system of sample that volume is little than simple mechanism, can realize at short notice that the robotization sample introduces, and reach low consumption, high-throughout effect; The present invention has a good application prospect.
Description of drawings
Fig. 1 is the rotating micro-example auto-introducing device synoptic diagram of the embodiment of the invention, among the figure 1, stepper motor, and 2, integrated sample disc, 3, liquid storage tank, 4, hole, 5, the kapillary sample probe, 6, liquid storage pipe, 7, electrode, 8, high-voltage power supply.
Fig. 2 rests on the synoptic diagram in the liquid storage tank hole for kapillary sample probe in the embodiment of the invention, among the figure 3, liquid storage tank, and 4, hole, 5, the kapillary sample probe, 9, the coat of metal, 10, sample solution.
Fig. 3 is the arginine and the leucic record spectrogram of continuous 7 the sample introduction separation detection FITC marks of rotating micro-example auto-introducing device analytic system of the employing embodiment of the invention; The arginine of a, FITC mark among the figure (0.4 μ mol/L); B, FITC, the leucine of c, FITC mark (0.8 μ mol/L).
Fig. 4 varies continuously for the rotating micro-example auto-introducing device analytic system that adopts the embodiment of the invention and separates the amino acid whose record spectrogram of variable concentrations; Leucine (the 0.2 μ mol/L) potpourri of arginine of d, FITC mark among the figure (0.1 μ mol/L) and FITC; Leucine (the 0.4 μ mol/L) potpourri of arginine of e, FITC mark (0.2 μ mol/L) and FITC, the leucine potpourri (0.8 μ mol/L) of arginine of f, FITC mark (0.4 μ mol/L) and FITC.
Embodiment
Fluorescence labeling material in the embodiment of the invention is FITC, i.e. fluorescein isothiocynate.
Embodiment 1
Rotating micro-example auto-introducing device is as shown in Figure 1, and integrated sample disc 2 is a gear shape, and diameter is 7cm; Thickness is 8cm, and the edge is uniform-distribution with 20 drum shape liquid storage tanks 3, and each liquid storage tank 3 internal diameter is 3.5mm; The degree of depth is 7mm, and the bottom surface is on the same surface level, and each liquid storage tank 3 is evenly distributed on the integrated sample disc 2; The side of disk body and liquid storage tank inwall minor increment are 2mm, and each liquid storage tank 3 exterior bottom has the long-pending hole 4 of an identical shaped same cross-sectional, and hole 4 width are 1.2mm; The degree of depth is 2.0mm, and each hole 4 is inner and integrated sample disc 2 outsides connection with liquid storage tank, and is on the same surface level; Integrated sample disc 2 is fixed on the axle of stepper motor 1, with the axle concentric of stepper motor 1.Wherein integrated sample disc adopts plastics to be formed by machining.
High-voltage power supply 8 is a DC high-voltage power supply; High-voltage power supply 8 is anodal through lead connection electrode 7; The sample export end of electrode 7 and kapillary sample probe 5 is inserted in the liquid storage pipe 6, and high-voltage power supply 8 negative poles are wired to the sample inlet end of kapillary sample probe 5.Splendid attire buffer solution in the liquid storage pipe 6 wherein.Electrode 7 is a platinum electrode.
The kapillary sample probe of rotating micro-example auto-introducing device of the present invention stops as shown in Figure 2 in liquid storage tank; Kapillary sample probe 5 applies the fused quartz kapillary of amorphous teflon for outer wall; Its internal diameter is 50 μ m, external diameter 365 μ m, the outer wall sputter layer of metal coating 9; As electrode, the coat of metal 9 thickness are 1 μ m; When integrated sample disc 2 is rotated, do not contact kapillary sample probe 5, and kapillary sample probe 5 can be inserted into the fluid sample 10 that places in the liquid storage tank 3, simultaneously the coat of metal 9 contact liq samples 10.
Adopt above-mentioned rotating micro-example auto-introducing device that sample is analyzed, concrete steps are:
Start stepper motor 1; Integrated sample disc 2 is rotated with stepper motor 1, and rotating speed is 3rad/s, and stops certain hour in the back that turns an angle; Kapillary sample probe 5 contacts with sample solution in the hole 4 during stop; Apply certain DC voltage through high-voltage power supply 8 at kapillary sample probe 5 two ends, sample solution gets in the kapillary sample probe 5 under electric field action, promptly accomplishes the sample introduction process; Kapillary sample probe 5 is used as the capillary electrophoresis separation passage: in kapillary sample probe 5, sample separates under effect of electric field, and through the pick-up unit analysis result.When stepper motor 1 continues to rotate the back when stopping next time, kapillary sample probe 5 contacts with sample solution in the next hole.
Each liquid storage tank 3 interval in integrated sample disc 2 add buffer solution and different sample solutions; When integrated plastic sample disc 2 is rotated under stepper motor 1 drives; Kapillary sample probe 5 alternately contacts with different sample solutions with buffer solution, under effect of electric field, accomplishes the process of varying; Rotate continuously sample disc, can realize continuous auto injection and vary, need be by any pump and valve, and the time of varying less than 0.2s, the sample solution consumption is the nL level.
Fig. 3 is for adopting the arginine and the leucic record spectrogram of above-mentioned rotating micro-example auto-introducing device and continuous 7 the sample introduction separation detection FITC marks of microcurrent controlled capillary tube electrophoresis analytic system; A is the arginine (0.4 μ mol/L) of FITC mark, and b is FITC, and c is the leucine (0.8 μ mol/L) of FITC mark; Kapillary sample probe 5 length are 7.5cm, and wherein effectively separation length is 6.0cm; Runtime buffer solution is the sodium tetraborate buffer solution (pH9.2) of 10mmol/L; Sample introduction with separate electric field intensity and be 320V/cm; Sample injection time is 1.0s, and the sample size of introducing is 1.3nL.
Fig. 4 separates the amino acid whose record spectrogram of variable concentrations for adopting above-mentioned rotating micro-example auto-introducing device to vary continuously with the microcurrent controlled capillary tube electrophoresis analytic system.D is the arginine (0.1 μ mol/L) of FITC mark and the leucine potpourri (0.2 μ mol/L) of FITC mark; E is the arginine (0.2 μ mol/L) of FITC mark and the leucine potpourri (0.4 μ mol/L) of FITC mark, and f is the arginine (0.4 μ mol/L) of FITC mark and the leucine potpourri (0.8 μ mol/L) of FITC mark.
The integrated sample disc 2 of rotating micro-example auto-introducing device is a gear shape, and diameter is 1cm, and thickness is 0.1cm; The edge is uniform-distribution with a drum shape liquid storage tank, and each liquid storage tank internal diameter is 0.5mm, and the degree of depth is 2mm; The bottom surface is on the same surface level, and each liquid storage tank is evenly distributed on the integrated sample disc, and the side of disk body and liquid storage tank inwall minor increment are 2mm; Each liquid storage tank exterior bottom has the long-pending hole of an identical shaped same cross-sectional, and pore width is 0.5mm, and the degree of depth is 2.0mm; Each hole is communicated with the inner and integrated sample disc of the liquid storage tank outside, and is on the same surface level; Integrated sample disc is fixed on the axle of stepper motor, with the axle concentric of stepper motor.
The kapillary sample probe is the fused quartz kapillary that outer wall applies amorphous teflon, and its internal diameter is 1 μ m, external diameter 5 μ m, the outer wall sputter layer of metal coating, as electrode, metal layer thickness is 0.1 μ m; When integrated sample disc is rotated, do not contact the kapillary sample probe, and the kapillary sample probe can be inserted into the fluid sample that places in the liquid storage tank, simultaneously coat of metal contact liq sample.
Adopt above-mentioned rotating micro-example auto-introducing device that sample is analyzed, mode is with embodiment 1, and rotating speed is 2rad/s, and the time of varying, the sample solution consumption was the nL level less than 0.2s.
The integrated sample disc 2 of rotating micro-example auto-introducing device is a gear shape, and diameter is 100cm, and thickness is 10cm; The edge is uniform-distribution with 500 drum shape liquid storage tanks, and each liquid storage tank internal diameter is 5mm, and the degree of depth is 50mm; The bottom surface is on the same surface level, and each liquid storage tank is evenly distributed on the integrated sample disc, and the side of disk body and liquid storage tank inwall minor increment are 5mm; Each liquid storage tank exterior bottom has the long-pending hole of an identical shaped same cross-sectional, and pore width is 0.5mm, and the degree of depth is 5mm; Each hole is communicated with the inner and integrated sample disc of the liquid storage tank outside, and is on the same surface level; Integrated sample disc is fixed on the axle of stepper motor, with the axle concentric of stepper motor.
The kapillary sample probe is the fused quartz kapillary that outer wall applies amorphous teflon, and its internal diameter is 1000 μ m, external diameter 5000 μ m, the outer wall sputter layer of metal coating, as electrode, metal layer thickness is 200 μ m; When integrated sample disc is rotated, do not contact the kapillary sample probe, and the kapillary sample probe can be inserted into the fluid sample that places in the liquid storage tank, simultaneously coat of metal contact liq sample.
Adopt above-mentioned rotating micro-example auto-introducing device that sample is analyzed, mode is with embodiment 1, and rotating speed is 5rad/s, and the time of varying, the sample solution consumption was the nL level less than 0.2s.
Claims (8)
1. rotating micro-example auto-introducing device; Comprise integrated sample disc, kapillary sample probe, stepper motor, high-voltage power supply, electrode, liquid storage pipe; It is characterized in that: integrated sample disc is a gear shape; Promptly be evenly distributed with liquid storage tank, have a hole on each liquid storage tank in the circular disk body outer; The sample inlet end of kapillary sample probe can be inserted in the hole of a liquid storage tank of integrated sample disc; Integrated sample disc is fixed on the axle of stepper motor, with the axle concentric of stepper motor; High-voltage power supply is a DC high-voltage power supply; A power supply wherein utmost point passes through the lead connection electrode; The sample export end of this electrode and kapillary sample probe is inserted in the liquid storage pipe; Another utmost point of power supply connects the sample inlet end of kapillary sample probe through lead, and the connected mode at the two poles of the earth is according to by the electrically charged switching of analytic sample; The sample inlet end of kapillary sample probe is when the rotation of integrated sample disc, and the kapillary sample probe does not contact the disk body of integrated sample disc; Kapillary sample probe material is fused quartz kapillary or high molecular polymer kapillary, and capillary inner diameter is 1～1000 μ m, wherein fused quartz outer wall coating polyimide capillaceous or amorphous teflon; The outer wall of the sample inlet end of kapillary sample probe scribbles one or more layers coat of metal, and coat of metal gross thickness is 0.1～200 micron, and the coat of metal on the sample inlet end of kapillary sample probe is connected through lead with high-voltage power supply.
2. rotating micro-example auto-introducing device according to claim 1 is characterized in that described integrated sample disc diameter is 1～100cm, and thickness is 0.1～10cm.
3. rotating micro-example auto-introducing device according to claim 1; It is characterized in that described each liquid storage tank is evenly distributed on the integrated sample disc; Said hole is positioned at each liquid storage tank exterior bottom; Each hole is inner and sample disc outside connection with liquid storage tank, and each hole is on the same surface level; The liquid storage tank diameter is 0.5～5mm, and the degree of depth is 2～50mm; Wide 0.5～the 5mm of hole.
4. the method for application of the described a kind of rotating micro-example auto-introducing device of claim 1; It is characterized in that: integrated sample disc is rotated with stepper motor; And stopping certain hour in the back that turns an angle, the sample inlet end of kapillary sample probe and the coat of metal contact with sample solution in the hole during stop, apply certain DC voltage through high-voltage power supply at kapillary sample probe two ends; Sample gets in the kapillary sample probe under electric field action, promptly accomplishes the sample introduction process.
5. the method for application of a kind of rotating micro-example auto-introducing device according to claim 4; It is characterized in that the kapillary sample probe is as the capillary electrophoresis separation passage; In the kapillary sample probe; Adopt the sample of tagged under effect of electric field, to separate, utilize the liquid core waveguide phenomenon, through laser vertical irradiation kapillary sample probe; The exciting light that fluorescent material produces axially conducts along the kapillary sample probe, and passes to the pick-up unit analysis result at the sample export end of kapillary sample probe.
6. the method for application of a kind of rotating micro-example auto-introducing device according to claim 4; It is characterized in that integrated sample disc rotates with the angular velocity below the 10rad/s under stepper motor drives; Because the surface tension effects between solution and the liquid storage pool wall, the solution that is stored in the liquid storage tank can not flow out from hole because of centrifugal action.
7. the method for application of a kind of rotating micro-example auto-introducing device according to claim 4; It is characterized in that each liquid storage tank interval adds buffer solution and different sample solutions; When integrated sample disc is rotated under stepper motor drives; The kapillary sample probe alternately contacts with different sample solutions with buffer solution, under effect of electric field, accomplishes the process of varying; Rotate integrated sample disc continuously, can realize continuous auto injection and vary.
8. the method for application of a kind of rotating micro-example auto-introducing device according to claim 4, its characteristic through the rotating micro-example auto-introducing device auto injection with vary, the time of varying, the sample solution consumption was the nL level less than 0.2s.
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| CN105092677B (en) * | 2014-05-06 | 2019-02-26 | 窦晓鸣 | Movable electrophoresis chip sample injection method and the sampling device suitable for this method |
| CN104280374B (en) * | 2014-10-29 | 2017-08-25 | 广州蓝勃生物科技有限公司 | A kind of rotating disc type entry dry type fluorescence detector |
| CN106645092B (en) * | 2017-02-24 | 2023-09-19 | 北京本立科技有限公司 | Liquid core waveguide Raman spectrum detection device based on centrifugation |
| US20220344139A1 (en) * | 2019-02-19 | 2022-10-27 | Shimadzu Corporation | Mass spectrometer |
| CN112858704A (en) * | 2020-12-31 | 2021-05-28 | 盐城工学院 | Biological gene detection device with rotatable sample holder |
| CN113252763A (en) * | 2021-04-21 | 2021-08-13 | 上海应用技术大学 | Multi-channel automatic sample introduction device for capillary electrophoresis fluorescence analysis |
| CN113358883A (en) * | 2021-05-19 | 2021-09-07 | 扬州大学 | Micro-fluidic device for detecting plateau disease |
| CN114453190B (en) * | 2021-09-24 | 2023-05-26 | 湖南红鑫通信技术有限责任公司 | Integrated dispensing mechanism for manufacturing and processing electronic equipment and application method thereof |
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