CN101396482B - Traditional Chinese medicine preparation for treating diabetes - Google Patents
Traditional Chinese medicine preparation for treating diabetes Download PDFInfo
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- CN101396482B CN101396482B CN200810218897XA CN200810218897A CN101396482B CN 101396482 B CN101396482 B CN 101396482B CN 200810218897X A CN200810218897X A CN 200810218897XA CN 200810218897 A CN200810218897 A CN 200810218897A CN 101396482 B CN101396482 B CN 101396482B
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Abstract
The invention belongs to the technical field of drugs, in particular to a Chinese materia medica preparation which is used for resisting diabetes and made by combining total terpenoids of discolor cinquefoil herb with tea polysaccharides based on a certain proportion; animal tests prove that the Chinese materia medica preparation for treating diabetes can effectively reduce the food intake, weight, blood sugar and content of glycosylated hemoglobin in blood (P<0.01) of laboratory mice with type II diabetes induced by streptozotocin, and improves the glucose tolerance; at the same time, the Chinese materia medica preparation for treating diabetes obviously reduces the blood sugar level of mouse models with diabetes induced by tetraoxypyrimidine; the Chinese materia medica preparation has obvious ability for resisting type I diabetes and type II diabetes; the Chinese materia medica preparation uses the combination of the total terpenoids of discolor cinquefoil herb and tea polysaccharides, thus developing powerful cooperative antidiabetic functions.
Description
Technical field:
The invention belongs to technical field of pharmaceuticals, particularly a kind of anti-diabetic Chinese medicine preparation.
Background technology:
Diabetes (full name: be diabetes mellitus) because the h and E factor interaction; Cause that absolute or relative hyposecretion of insulin and target tissue cell reduce insulin sensitivity; Causing a series of metabolism disorder syndromes such as protein, fat, power and water Xie Zhi, wherein is outstanding feature with the hyperglycemia.Performance such as polyuria, polydipsia, polyphagia can appear in clinical model case, become thin, i.e. " three-many-one-little " symptom.Diabetes are one of modal chronic diseases.The Along with people's growth in the living standard, the increase of aged tendency of population and fat incidence rate, the sickness rate of diabetes is ascendant trend year by year.Diabetes reach 2% at the sickness rate of China, and according to statistics, the diabetics made a definite diagnosis of China reaches 4,000 ten thousand, and with annual 1000000 speed increase.Wherein, the type i diabetes patient accounts for 10%, and the type ii diabetes patient accounts for 90%.The appearance of chronic complicating diseases is seldom arranged in type i diabetes patient 5 years after making a definite diagnosis, and on the contrary, the type ii diabetes patient had just had chronic complicating diseases to take place before making a definite diagnosis.According to statistics, have the 50% new type ii diabetes patient who diagnoses to have one or more chronic complicating diseases, some patient just finds to suffer from diabetes because of complication.Global diabetics was estimated about 3,000 ten thousand people in 1985.Should numeral break through 13,500 ten thousand to nineteen ninety-five.WHO prediction at present will rise to surprising 30,000 ten thousand in 2025.This present situation makes the health care resource that is exclusively used in diabetes constantly increase (www.who.int).Nearly 1,570 ten thousand people of the U.S. suffer from diabetes, account for population 5.9%, and wherein 2/3 suffers from type ii diabetes.There are every day 2200 people to be diagnosed as diabetes approximately.Nearly 798,000 people of calendar year 2001 by diagnosis suffer from diabetes (American Diabetes Association, www.diabetes.org).In the U.S., diabetes are that disease causes dead the sixth-largest reason.According to the death certificate data, caused 198140 people dead because of diabetes in 1996.In the U.S., diabetes are one of maximum health problems of cost.The health care directly related with treating diabetes and other cost, and the forfeiture productivity's cost reach 9,800,000,000 dollars every year.Global antidiabetic medicine in 1999 is sold and is amounted to about 12.7 hundred million dollars, and the expection annual growth rate is 15%.
Diabetes are a kind of chronic diseases that are difficult to cure.Treatment of diabetes is mainly taked to be the basis with Diet Therapy and physical training, give the complex treatment measure of antidiabetic drug treatment according to the different state of an illness.Though blood sugar reducing function is obvious, also there are many side effect in the insulin, sulfonylurea, the biguanides that extensively adopt at present.So urgent need development at present and exploitation selectivity are strong, have no side effect efficient green anti-diabetic new drug.
Summary of the invention:
The objective of the invention is to improve many side effect that existing treatment diabetes medicament brings, it is strong to develop a kind of selectivity, has no side effect efficient green anti-diabetic new drug.
In order to realize the object of the invention, we have done following research: we find that Herba Potentillae Discoloris is a kind of natural wild medical herbs when research, it spreads all over all parts of the country, is distributed widely in China the Northeast.According to the Compendium of Material Medica record, it has the effect of heat clearing away, detoxifcation, hemostasis, detumescence, and discovery among the people in recent years has certain curative effect with Herba Potentillae Discoloris treatment diabetes.And tea is of long duration as traditional beverage of China, and recently according to the scholar, with Folium Camelliae sinensis merceration one day, its immersion of usefulness was oral, can reduce the hyperglycemia value of diabetics.Contain the synthetic material of promotion insulin in the Folium Camelliae sinensis, also contain the polysaccharose substance that to remove sugar in the blood.
Technical scheme of the present invention does
1, proportioning raw materials: anti-diabetic new drug Chinese medicine preparation of the present invention is made up of total terpene of Herba Potentillae Discoloris and Folium Camelliae sinensis polysaccharide two flavor Chinese medicines.The ratio of total terpene of Herba Potentillae Discoloris and Folium Camelliae sinensis polysaccharide is: 1:1～1:10 or 10:1.
2, feedstock production:
1) preparation of the total terpene dry powder of raw material Herba Potentillae Discoloris
The preparation technology of the total terpene of raw material Herba Potentillae Discoloris is: elder generation cleans the Herba Potentillae Discoloris that collection is grown in Hubei Province, dry, and section and pulverizing, pulverizing is 25-50 orders.The Herba Potentillae Discoloris that takes by weighing after 50 grams are pulverized adds 500 milliliter of 80% alcohol reflux 3 times; Reclaim ethanol and concentrated extract and get paste; Adopt No. 90 petroleum ether dissolutions and the above-mentioned paste that suspends to carry out degreasing process then, centrifugation separates No. 90 the petroleum ether solvent part; Get and precipitate part and fully that No. 90 residual petroleum ether volatilizations are clean, after drying, get 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris.
2) preparation of raw material Folium Camelliae sinensis polysaccharide dry powder
The preparation technology of Folium Camelliae sinensis polysaccharide dry powder is: under 4 ℃～25 ℃ conditions, soaked 50 gram Folium Camelliae sinensis 1 to 24 hour with 2000 ml waters, soak the back that finishes and cross filtering Folium Camelliae sinensis medicinal residues with 2 layers of husky cloth.Use the ethanol precipitation Folium Camelliae sinensis polysaccharide of 80% concentration then and get 500 milligrams of Folium Camelliae sinensis polysaccharide dry powder through vacuum tea polysaccharide dry (-140 ℃).
3) preparation of medicaments of the present invention
Take by weighing 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris and 500 milligrams of Folium Camelliae sinensis polysaccharide dry powder and be dissolved in 25 ml physiological salines, through 0.2 micron pore size membrane filtration, sterilization; Preparation cost invention antidiabetic oral liquid.Its concentration contains 4 milligrams of total terpenes of Herba Potentillae Discoloris and 20 milligrams of Folium Camelliae sinensis polysaccharide dry weight things for every milliliter.The former medicine dry powder of anti-diabetic of the present invention can be used as the crude drug of diabetes medicament, processes anti-diabetic tablet, electuary, capsule preparations, dropping pill formulation and oral liquid formulations with pharmaceutically suitable carrier or excipient.
Advantage of the present invention and good effect: a kind of anti-diabetic Chinese medicine preparation is combined by total terpene of Herba Potentillae Discoloris and Folium Camelliae sinensis polysaccharide according to a certain percentage.Find that through animal experiment this treatment diabetes Chinese medicine preparation can reduce the content of glycolated hemoglobin in food ration, body weight, blood glucose and the blood that streptozotocin brings out experimental type ii diabetes mice (P < 0.01) very effectively, and improve glucose tolerance.Simultaneously, this treatment diabetes Chinese medicine preparation reduces the inductive diabetic mice model of alloxan blood sugar level very significantly.These results show that Chinese medicine preparation of the present invention has the ability of anti-I type diabetes and type ii diabetes; And the treatment ability obviously is superior to total terpene of single Herba Potentillae Discoloris and single Folium Camelliae sinensis polysaccharide, and the proof total terpene of Herba Potentillae Discoloris and Folium Camelliae sinensis polysaccharide united use and had powerful concertedness antidiabetic effect from test.
Description of drawings:
Fig. 1 is the influence to the type ii diabetes mouse blood sugar of antidiabetic oral liquid of the present invention, the total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid
Fig. 2 is the influence to the mice carbohydrate tolerance of antidiabetic oral liquid of the present invention, the total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid
Fig. 3 is the influence to the mice glycolated hemoglobin of antidiabetic oral liquid of the present invention, the total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid
The specific embodiment:
Being most preferred embodiment of the present invention below, further specifying the present invention through embodiment, is not to be limited to this to practical range of the present invention.
Embodiment 1
The first step: the preparation of the total terpene dry powder of raw material Herba Potentillae Discoloris
Fresh Herba Potentillae Discoloris is cleaned, dries, cut into slices and pulverizing is 35 orders; The Herba Potentillae Discoloris that takes by weighing after 50 grams are pulverized adds the 500ml80% alcohol reflux 3 times, reclaims ethanol; Concentrated extract gets paste; Carry out degreasing process with No. 90 petroleum ether dissolutions and the above-mentioned paste that suspends, centrifugation separates No. 90 the petroleum ether solvent part; Fully that No. 90 residual petroleum ether volatilizations are clean, get the deposition part, after drying, get 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris.
Second step: the preparation of raw material Folium Camelliae sinensis polysaccharide dry powder
In temperature is under 4 ℃ of temperature conditions, restrains Folium Camelliae sinensis 5 hours with 400ml cold water soak 10, soaks the back and crosses filtering Folium Camelliae sinensis medicinal residues with 2 layers of husky cloth; With the Aqua Folium Camelliae sinensis after filtering with 80% ethanol precipitation Folium Camelliae sinensis polysaccharide, and with Folium Camelliae sinensis polysaccharide vacuum drying.Baking temperature is-140 ℃, gets 100 milligrams of Folium Camelliae sinensis polysaccharide dry powder.
The 3rd step: the preparation of anti-diabetic Chinese medicine preparation
Taking by weighing 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris and 100 milligrams of Folium Camelliae sinensis polysaccharide dry powder mixes in proportion the Folium Camelliae sinensis polysaccharide dry powder of the total terpene dry powder of the Herba Potentillae Discoloris of above-mentioned first step gained and the second step gained; Obtain anti-diabetic Chinese medicine dry powder, anti-diabetic Chinese medicine dry powder and pharmaceutical carrier or excipient are processed tablet, electuary, capsule preparations, dropping pill formulation and oral liquid formulation.
Embodiment 2
The first step: the preparation of the total terpene dry powder of raw material Herba Potentillae Discoloris
Fresh Herba Potentillae Discoloris is cleaned, dries, cut into slices and pulverizing is 25 orders; The Herba Potentillae Discoloris that takes by weighing after 50 grams are pulverized adds the 500ml80% alcohol reflux 3 times, reclaims ethanol; Concentrated extract gets paste; Carry out degreasing process with No. 90 petroleum ether dissolutions and the above-mentioned paste that suspends, centrifugation separates No. 90 the petroleum ether solvent part; Fully that No. 90 residual petroleum ether volatilizations are clean, get the deposition part, after drying, get 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris.
Second step: the preparation of raw material Folium Camelliae sinensis polysaccharide dry powder
In temperature is under 4 ℃ of temperature conditions, restrains Folium Camelliae sinensis 10 hours with 2000ml cold water soak 50, soaks the back and crosses filtering Folium Camelliae sinensis medicinal residues with 2 layers of husky cloth; With the Aqua Folium Camelliae sinensis after filtering with 80% ethanol precipitation Folium Camelliae sinensis polysaccharide, and with Folium Camelliae sinensis polysaccharide vacuum drying.Baking temperature is-140 ℃, gets 500 milligrams of Folium Camelliae sinensis polysaccharide dry powder.
The 3rd step: the preparation of anti-diabetic Chinese medicine oral liquid
Take by weighing 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris and 500 milligrams of Folium Camelliae sinensis polysaccharide dry powder, be dissolved in 25 ml physiological salines,, be prepared into antidiabetic oral liquid through 0.2 micron pore size membrane filtration, sterilization.Its concentration contains 4 milligrams of total terpenes of Herba Potentillae Discoloris and 20 milligrams of Folium Camelliae sinensis polysaccharide dry weight things for every milliliter.
Embodiment 3
The first step: the preparation of the total terpene dry powder of raw material Herba Potentillae Discoloris
Fresh Herba Potentillae Discoloris is cleaned, dries, cut into slices and pulverizing is 50 orders; The Herba Potentillae Discoloris that takes by weighing after 50 grams are pulverized adds the 500ml80% alcohol reflux 3 times, reclaims ethanol; Concentrated extract gets paste; Carry out degreasing process with No. 90 petroleum ether dissolutions and the above-mentioned paste that suspends, centrifugation separates No. 90 the petroleum ether solvent part; Fully that No. 90 residual petroleum ether volatilizations are clean, get the deposition part, after drying, get 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris.
Second step: the preparation of raw material Folium Camelliae sinensis polysaccharide dry powder
In temperature is under 10 ℃ of temperature conditions, restrains Folium Camelliae sinensis 20 hours with 4000ml cold water soak 100, soaks the back and crosses filtering Folium Camelliae sinensis medicinal residues with 2 layers of husky cloth; With the Aqua Folium Camelliae sinensis after filtering with 80% ethanol precipitation Folium Camelliae sinensis polysaccharide, and with Folium Camelliae sinensis polysaccharide vacuum drying.Baking temperature is-140 ℃, gets 500 milligrams of Folium Camelliae sinensis polysaccharide dry powder.
The 3rd step: the preparation of anti-diabetic Chinese medicine oral liquid
Take by weighing 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris and 500 milligrams of Folium Camelliae sinensis polysaccharide dry powder, be dissolved in 25 ml physiological salines,, be prepared into antidiabetic oral liquid through 0.2 micron pore size membrane filtration, sterilization.Its concentration contains 4 milligrams of total terpenes of Herba Potentillae Discoloris and 20 milligrams of Folium Camelliae sinensis polysaccharide dry weight things for every milliliter.
Comparative study:
Antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are to the studies on acute toxicity of mice: the comparative sample preparation:
1) the total terpene oral liquid of Herba Potentillae Discoloris
Take by weighing 100 milligrams of total terpene dry powder of Herba Potentillae Discoloris and be dissolved in 25 ml physiological salines,, thereby be prepared into the total terpene oral liquid of Herba Potentillae Discoloris through the sterilization of 0.2 micron pore size membrane filtration.Its concentration contains the 4 milligrams of total terpene dry weight of Herba Potentillae Discoloris things for every milliliter.
2) Folium Camelliae sinensis polyoses oral liquid
Take by weighing 500 milligrams of Folium Camelliae sinensis polysaccharide dry powder and be dissolved in 25 ml physiological salines,, thereby be prepared into the Folium Camelliae sinensis polyoses oral liquid through the sterilization of 0.2 micron pore size membrane filtration.Every milliliter of dry weight thing that contains 20 milligrams of its concentration.
Get the kunming mice (purchase) in six group of 8 age in week in Nanfang Medical Univ zooscopy center; Each 10 of first group and second groups (5 male and 5 female); Irritate stomach with single dose 1.2g/kg and multidose 0.24g/kg (QD X15) antidiabetic oral liquid of the present invention respectively, observed for 1 week and 4 weeks then respectively.Each 10 of the 3rd group and the 4th groups (5 male with 5 female) respectively with single dose 1g/kg and the total terpene oral liquid of multidose 0.2g/kg (QDX15) Herba Potentillae Discoloris, observed for 1 all and 4 weeks respectively then.Each 10 of the 5th group and the 6th groups (5 male with 5 female) give the water extract oral liquid with single dose 1g/kg with multidose 0.2g/kg (QD X15) Folium Camelliae sinensis polyoses oral liquid filling stomach respectively, observe for 1 all and 4 weeks then respectively.In this test, weighed in per two days.After the off-test, will be tried mice and put to death, carried out pathological analysis.
1, antidiabetic oral liquid of the present invention, the total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are induced the influence research of type ii diabetes mice to streptozotocin:
1) mice (buying the Experimental Animal Center in Nanfang Medical Univ) of the foundation of type ii diabetes mouse model: 26-28 gram body weight fed for 1 week to conform with normal diet earlier; Grow 80 of normal mices of picked at random sufficient amount therefrom; Feed 4 weeks of high-sugar-fat-diet; Lumbar injection is bought the streptozotocin (STZ) in Sigma company then; The injection volume of every mice is respectively 100mg/kg (streptozotocin is diluted in the citric acid injection solution of 0.05mol/L); Continue simultaneously to feed 4 weeks of high-sugar-fat-diet (high glucose and high fat feedstuff: add 10% yolk powder, 10% lard stearin, 1% cholesterol, 0.5% NaTDC in the normal feedstuff), the citric acid solution of model control group injection 0.05mol/L is monitored the mouse blood sugar value weekly.Be as the criterion with the 4th all measured values, choose mice that blood glucose reaches diabetes progression and have a similar blood sugar level as the type ii diabetes model mice.
2) antidiabetic oral liquid of the present invention, the total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are induced the influence research of type ii diabetes mice to streptozotocin:
Model mice is divided into four groups of A, B, C, D at random; Every group 10; Wherein the A group is model control group; Every day every mouse stomach 0.2ml normal saline, three groups of B, C, D every of every day irritate stomach 0.2ml antidiabetic oral liquid of the present invention (0.24g/kg body weight), the total terpene oral liquid of Herba Potentillae Discoloris (0.2g/kg body weight) and Folium Camelliae sinensis polyoses oral liquid (0.2g/kg body weight) respectively.Each organizes mice continuous irrigation stomach 36 days, overnight fasting (15h) then, and eyeball is got blood, measures blood glucose and the saccharification hemoglobin content of each mice.Irritating stomach after 11 days, each organizes the mice overnight fasting, and the ophthalmic corner of the eyes is got blood; Measure blood glucose; Respectively organize mice then by 2g/kg body weight lumbar injection D/W,, measure the mice glucose tolerance respectively at the blood glucose value of injection back 0,10,30,60,90,120 minute mensuration mice.
2, antidiabetic oral liquid of the present invention, the total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are induced the influence research of type i diabetes mice to the chain alloxan:
1) mice (buying the Experimental Animal Center in Nanfang Medical Univ) of the foundation of zoopery type i diabetes model: 26-28 gram body weight, fasting 16h prepares to give the alloxan modeling.Alloxan solution faces that to use preceding preparation, concentration be 12mg/ml.Administering mode is a lumbar injection, dosage 120mg/ (kg body weight), and after the week, after the 8th day jejunitas 3 hours, the blood sampling of eye socket endocanthion, the centrifuging and taking upper serum was used determination of glucose oxidase blood glucose in 24 hours.Get blood glucose value>diabetic mice of 12mmol/L divides into groups to carry out antidiabetic oral liquid blood sugar lowering experiment of the present invention.
2) zoopery is divided into groups and is handled: get blood glucose value>40 of the diabetic mices of 12mmol/L; Model mice is divided into four groups of A, B, C, D at random; Every group 10, wherein A group is model control group, every day every mouse stomach 0.2ml normal saline; B, C, every filling three groups of difference of D every day stomach 0.2ml antidiabetic oral liquid of the present invention (0.24g/kg body weight), the total terpene oral liquid of Herba Potentillae Discoloris (0.2g/kg body weight) and Folium Camelliae sinensis polyoses oral liquid (0.2g/kg body weight).10 of normal mouses are as matched group.Administering mode is for irritating stomach, every day 1 time, 30 days time.After 30 days, survey blood glucose value, condition and method are the same.Claim weekly in 30 days respectively to organize mice body weight on an empty stomach, observe body weight change.
Comparative test result
1, antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are to the studies on acute toxicity of mice:
Get the kunming mice (purchase) in six group of 8 age in week in Nanfang Medical Univ zooscopy center; Each 10 of first group and second groups (5 male and 5 female); Irritate stomach with single dose 1.2g/kg and multidose 0.24g/kg (QD X15) antidiabetic oral liquid of the present invention respectively, observed for 1 week and 4 weeks then respectively.Each 10 of the 3rd group and the 4th groups (5 male with 5 female) respectively with single dose 1g/kg and the total terpene oral liquid of multidose 0.2g/kg (QDX15) Herba Potentillae Discoloris, observed for 1 all and 4 weeks respectively then.Each 10 of the 5th group and the 6th groups (5 male with 5 female) give the water extract oral liquid with single dose 1g/kg with multidose 0.2g/kg (QD X15) Folium Camelliae sinensis polyoses oral liquid filling stomach respectively, observe for 1 all and 4 weeks then respectively.In this test, all do not observe toxicity, all are tried the mice well-grown, none death.Therefore giving antidiabetic oral liquid of the present invention is the anti-diabetic new drug with very great development prospect.
2, antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are induced the influence research of type ii diabetes mice to streptozotocin:
1) antidiabetic oral liquid of the present invention; Total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are to the influence of type ii diabetes mice mouse blood sugar: antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are seen Fig. 1 to the result that influences of blood glucose in diabetic mice.By visible among the figure; Antidiabetic oral liquid of the present invention; Total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid were irritated stomach after 36 days, antidiabetic oral liquid group of the present invention (B group), the total terpene oral liquid of Herba Potentillae Discoloris group (C group) and Folium Camelliae sinensis polyoses oral liquid group (D group) and model control group (A group) discovery of comparing; A group mouse blood sugar has slight increase, but difference does not have significance (P>0.05) before and after the feeding; Blood sugar concentration has remarkable reduction (P < 0.01) before and after three groups of mouse feedings of B, C, D, and the B group descend than C, D group mouse blood sugar lower, between difference (P < 0.01) arranged significantly.
2) antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are to the influence of mice carbohydrate tolerance: antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are seen Fig. 2 to the influence of diabetic mice carbohydrate tolerance.Behind the injected in mice glucose in 120 minutes, B, C, three groups of mouse blood sugar concentration of D significantly are lower than A group (P < 0.01), and the B group is minimum.
3) antidiabetic oral liquid of the present invention; Total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are to the influence of mice glycolated hemoglobin: antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are seen Fig. 3 to the influence of diabetic mice glycolated hemoglobin.The content of B, C, three groups of mice glycolated hemoglobins of D all significantly is lower than A group (P < 0.01), and B group minimum (P>0.05).More than these results by showing that antidiabetic oral liquid of the present invention is the clinical candidate of a kind of very promising anti-gout novel medical, its anti-type ii diabetes obviously is superior to total terpene oral liquid of single Herba Potentillae Discoloris and single Folium Camelliae sinensis polyoses oral liquid.
3, antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are induced the influence research of type i diabetes mice to the chain alloxan:
Table 1 shows antidiabetic oral liquid of the present invention, and the total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid group and diabetic model group comparison, blood glucose reduction difference have statistical significance (P < 0.01), and antidiabetic oral liquid group blood sugar lowering ability of the present invention is the strongest.Experimental session; Observation through to the weight of animals can be known; The diabetic model group the weight of animals increasess slowly; The equal steady growth of all the other each treated animal body weight, but comparing difference not statistically significant (P>0.05) between each group can think that the total terpene of Herba Potentillae Discoloris and Folium Camelliae sinensis polysaccharide do not influence the body weight of diabetic mice.This result proves that further antidiabetic oral liquid of the present invention is a kind of new drug candidate of very efficacious therapy type i diabetes.
Table 1: antidiabetic oral liquid of the present invention, total terpene oral liquid of Herba Potentillae Discoloris and Folium Camelliae sinensis polyoses oral liquid are induced the influence (X ± S) contrast tabulation of type i diabetes mice to the chain alloxan
| Group | Number of animals | Before the experiment | After the experiment |
| The |
15 | 7.7±1.2 | 8.6±1.7 |
| Negative control group (A) | 15 | 17.1±3.1 | 15.8±2.3 |
| Antidiabetic oral liquid of the present invention (B) | 15 | 17.0±2.5 | 8.5±1.9 |
| The total terpene oral liquid of |
15 | 16.9±2.6 | 13.2±1.71 |
| The Folium Camelliae sinensis polyoses oral liquid | 15 | 16.8±3.1 | 11.3±2.1 |
The above only is a preferred embodiments of the present invention, so all equivalences of doing according to the described structure of patent claim of the present invention, characteristic and principle change or modify, includes in patent claim of the present invention.
Claims (1)
1. anti-diabetic Chinese medicine preparation, it is characterized in that: be made up of total terpene of Herba Potentillae Discoloris and Folium Camelliae sinensis polysaccharide, the weight ratio of total terpene of Herba Potentillae Discoloris and Folium Camelliae sinensis polysaccharide is: 1: 1～1: 10, this anti-diabetic Chinese medicine preparation was prepared by following steps:
The first step: the preparation of the total terpene dry powder of Herba Potentillae Discoloris
A), fresh Herba Potentillae Discoloris is cleaned, dries, cut into slices and pulverizing is 25～50 orders;
B), the Herba Potentillae Discoloris that crushes is added 80% alcohol reflux 3 times, recovery ethanol; Concentrated extract gets paste;
C), carry out degreasing process, centrifugation separates No. 90 the petroleum ether solvent part with No. 90 petroleum ether dissolutions and the above-mentioned paste that suspends;
D), fully totally, get the deposition part, drying gets the total terpene dry powder of Herba Potentillae Discoloris with No. 90 residual petroleum ether volatilizations;
Second step: the preparation of Folium Camelliae sinensis polysaccharide dry powder
A), under 4 ℃～25 ℃ temperature conditions, the Folium Camelliae sinensis 1～24 hour of being soaked in water soaks the back and crosses filtering Folium Camelliae sinensis medicinal residues with 2 layers of husky cloth;
B), the Aqua Folium Camelliae sinensis after will filtering is with 80% ethanol precipitation Folium Camelliae sinensis polysaccharide, and with Folium Camelliae sinensis polysaccharide vacuum drying, baking temperature is-140 ℃, Folium Camelliae sinensis polysaccharide dry powder;
The 3rd step: the preparation of anti-diabetic Chinese medicine preparation:
The Folium Camelliae sinensis polysaccharide dry powder of the total terpene dry powder of the Herba Potentillae Discoloris of above-mentioned first step gained and the second step gained is mixed in proportion, obtain anti-diabetic Chinese medicine dry powder.
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| CN200810218897XA CN101396482B (en) | 2008-11-04 | 2008-11-04 | Traditional Chinese medicine preparation for treating diabetes |
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| CN200810218897XA CN101396482B (en) | 2008-11-04 | 2008-11-04 | Traditional Chinese medicine preparation for treating diabetes |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958617A (en) * | 2006-11-28 | 2007-05-09 | 湖北丽益医药科技有限公司 | Tea polysaccharide, preparation method, and usage |
| CN101204466A (en) * | 2007-11-09 | 2008-06-25 | 邱日辉 | Novel antidiabetic |
-
2008
- 2008-11-04 CN CN200810218897XA patent/CN101396482B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1958617A (en) * | 2006-11-28 | 2007-05-09 | 湖北丽益医药科技有限公司 | Tea polysaccharide, preparation method, and usage |
| CN101204466A (en) * | 2007-11-09 | 2008-06-25 | 邱日辉 | Novel antidiabetic |
Non-Patent Citations (1)
| Title |
|---|
| 张俭等.翻白草活性成分的萃取.《食品工业科技》.2008,第29卷(第3期), * |
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| CN101396482A (en) | 2009-04-01 |
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