CN101396386A - Extraction method of radix bupleuri - Google Patents
Extraction method of radix bupleuri Download PDFInfo
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- CN101396386A CN101396386A CNA2008101599876A CN200810159987A CN101396386A CN 101396386 A CN101396386 A CN 101396386A CN A2008101599876 A CNA2008101599876 A CN A2008101599876A CN 200810159987 A CN200810159987 A CN 200810159987A CN 101396386 A CN101396386 A CN 101396386A
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Abstract
The invention relates to a method for extracting bupleurum. The invention aims to provide a new method for extracting the effective components of the bupleurum completely and is characterized in that the method for extracting the bupleurum comprises the working procedures of ethanol extraction, water extraction and purification. The invention has the beneficial effect of, compared with the prior art, effectively extracting the medical part of the bupleurum and improving the medical value of the bupleurum.
Description
Technical field
The invention belongs to this technical field of extracting method of Chinese medicine, belong to this technical field of extracting method of Radix Bupleuri especially.
Background technology
Radix Bupleuri is the dry root of umbelliferae bupleurum or Radix Bupeuri Scorzonerfolii..Its property of medicine hardship is slightly cold.Return liver, gallbladder meridian.Its deliver and in, yang invigorating, soothing liver-QI.Be used for cold, fever, alternate attack of chill and fever, insufficiency of the spleen chronic diarrhea, hang down and take off the treatment of proctoptosis in the uterus.The effective ingredient of Radix Bupleuri comprises non-volatile and the volatility part.Contain bupleurumol etc. in the volatile oil, it is to influenza, and bringing down a fever of disease such as general sense and malaria have certain effect.With distilled Radix Bupleuri residue, extracting solution that makes after treatment and distillate merge the injection of making, its calmness, and pain relieving, analgesic comparatively more remarkable than separately with distillate with effect antiinflammatory.Simultaneously, contained effective ingredient is the total Saponin of Radix Bupleuri in the non-volatile part of Radix Bupleuri, and pharmacology facts have proved that it has tangible central inhibitory action and antiinflammatory action.And can hinder acetylcholine esterase specifically and significant diuresis is arranged.So the comprehensive function of bottoms has not only improved the curative effect of Chinese medicine preparation, and the certain meaning of the resource aspect that puts everything to its best use oil.
The extracting method of Radix Bupleuri covers placement 8 hours for exsiccant Radix Bupleuri root is worn into coarse powder with the fully drenched back of cold water in the prior art.Pack into and distill in the vapor distillation pot, collect simple distillation liquid redistillation, collect re-distilled liquid.The shortcoming of the method is the effective ingredient that the Radix Bupleuri extract prepared can not extract Radix Bupleuri fully.
Summary of the invention
The purpose of this invention is to provide a kind of new method that can fully extract the Radix Bupleuri effective ingredient, the technical solution adopted for the present invention to solve the technical problems is: a kind of extracting method of Radix Bupleuri comprises that alcohol extraction operation, water carries operation, purification process.
Described alcohol extraction operation is for being 50～80% ethanol extraction 0.5～5 hour with volumetric concentration with dry product Radix Bupleuri under room temperature, stirring condition, and Radix Bupleuri and alcoholic acid by weight/volume are 1:10～50; Filter, filtrate is condensed into the extractum that density is 1.03～1.08/ml.
Described water carry operation for the filtering residue of alcohol extraction operation under 50～60 ℃ condition, with water extraction 1～4 hour, the weight ratio of filtering residue and water was 1:10～20; Filter, filtrate is condensed into the extractum that density is 1.03～1.08/ml.In this operation,, can also add 1% tannin of filtering residue gross weight in order to improve the degree of saikoside.
Described purification process adds 80% n-butyl alcohol for after extractum that the alcohol extraction operation is prepared and water carries the prepared extractum mix homogeneously of operation, add 80% n-butyl alcohol and the condensed cream volume/weight ratio is 1:3～5; Put in the extractor, stir, place, get the n-butanol layer concentrating under reduced pressure.Condensed cream separates with silicagel column, and silica gel and applied sample amount weight ratio are no less than 0.5; Earlier with low amounts of water sweetening off and other water soluble ingredients, after change that use 50% ethanol elution, eluent flow rate be 3ml/min into, the eluent decompression recycling ethanol, Radix Bupleuri extract, the Radix Bupleuri extract prepared with the present invention can be used for injection of Radix Bupleuri.
The invention has the beneficial effects as follows: the present invention compared with prior art can effectively extract the medicinal part in the Radix Bupleuri, has improved the medical value of Radix Bupleuri.
The specific embodiment
The present invention is described in detail below in conjunction with embodiment.
The assay method of the total Saponin of Radix Bupleuri of the present invention is for to carry out assay with vanillin-concentrated sulphuric acid colorimetry.
Embodiment 1:
1, alcohol extraction operation: is 50% 2.5 ton ethanol extractions 5 hour with volumetric concentration with dry product Radix Bupleuri 50kg under room temperature, stirring condition, and filtrate is condensed into the extractum that density is 1.03～1.08/ml, reclaims ethanol.
2, water is put forward operation: the 50kg filtering residue of alcohol extraction operation under 50 ℃ condition, is added 1 ton in water, extracted 4 hours, filter, filtrate is condensed into the extractum that density is 1.03～1.08/ml.
3, purification process: after extractum that the alcohol extraction operation is prepared and water are carried the prepared extractum mix homogeneously of operation, add 80% n-butyl alcohol, institute adds 80% n-butyl alcohol and the condensed cream volume/weight ratio is 1:5; Put in the extractor, stirred 30 minutes, placement is spent the night, and gets n-butanol layer and concentrates.Condensed cream separates with silicagel column, silica gel and fluid extract weight ratio 1:1; Divide and other water soluble ingredients earlier with low amounts of water eluting desaccharide, after change that use 50% ethanol elution, eluent flow rate be 3ml/min into, the Saponin in the collection Radix Bupleuri gets final product.
The total Saponin of the Radix Bupleuri of embodiment 1 carries out assay with vanillin-concentrated sulphuric acid colorimetry and counts 2.22% with dry product Radix Bupleuri.
Embodiment 2:
1, alcohol extraction operation: is 80% 0.5 ton ethanol extraction 5 hour with volumetric concentration with dry product Radix Bupleuri 50kg under room temperature, stirring condition, and filtrate is condensed into the extractum that density is 1.03～1.08/ml, reclaims ethanol.
2, water is put forward operation: the 50kg filtering residue of alcohol extraction operation under 60 ℃ condition, is added 0.5 ton in water, extracted 1 hour, filter, filtrate is condensed into the extractum that density is 1.03～1.08/ml.
3, purification process: after extractum that the alcohol extraction operation is prepared and water are carried the prepared extractum mix homogeneously of operation, add 80% n-butyl alcohol, institute adds 80% n-butyl alcohol and the condensed cream volume/weight ratio is 1:5; Put in the extractor, stirred 30 minutes, placement is spent the night, and gets n-butanol layer and concentrates.Condensed cream separates with silicagel column, silica gel and fluid extract weight ratio 1:1; Divide and other water soluble ingredients earlier with low amounts of water eluting desaccharide, after change that use 50% ethanol elution, eluent flow rate be 3ml/min into, the Saponin in the collection Radix Bupleuri gets final product
The total Saponin of the Radix Bupleuri of embodiment 2 carries out assay with vanillin-concentrated sulphuric acid colorimetry and counts 2.31% with dry product Radix Bupleuri.
Embodiment 3:
Add 1% the tannin that accounts for the filtering residue gross weight except that carrying at water in the operation, all the other are identical with embodiment 2.
The total Saponin of the Radix Bupleuri of embodiment 3 carries out assay with vanillin-concentrated sulphuric acid colorimetry and counts 2.36% with dry product Radix Bupleuri.
Claims (4)
1, a kind of extracting method of Radix Bupleuri, the extracting method that it is characterized in that described Radix Bupleuri comprise that alcohol extraction operation, water carries operation, purification process.
2, the extracting method of a kind of Radix Bupleuri according to claim 1, it is characterized in that described alcohol extraction operation for being 50～80% ethanol extraction 0.5～5 hour with volumetric concentration with dry product Radix Bupleuri under room temperature, stirring condition, Radix Bupleuri and alcoholic acid by weight/volume are 1:10～50; Filter, filtrate is condensed into the extractum that density is 1.03～1.08/ml.
3, the extracting method of a kind of Radix Bupleuri according to claim 1, it is characterized in that described water carry operation for the filtering residue of alcohol extraction operation under 50～60 ℃ condition, with water extraction 1～4 hour, the weight ratio of filtering residue and water was 1:10～20; Filter, filtrate is condensed into the extractum that density is 1.03～1.08/ml, in this operation, can also be added to the tannin of filtering residue gross weight 1%.
4, the extracting method of a kind of Radix Bupleuri according to claim 1, it is characterized in that described purification process is for after extractum that the alcohol extraction operation is prepared and water carries the prepared extractum mix homogeneously of operation, the n-butyl alcohol of adding 80%, institute adds 80% n-butyl alcohol and the condensed cream volume/weight ratio is 1:3～5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101599876A CN101396386A (en) | 2008-11-14 | 2008-11-14 | Extraction method of radix bupleuri |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101599876A CN101396386A (en) | 2008-11-14 | 2008-11-14 | Extraction method of radix bupleuri |
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| CN101396386A true CN101396386A (en) | 2009-04-01 |
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| CNA2008101599876A Pending CN101396386A (en) | 2008-11-14 | 2008-11-14 | Extraction method of radix bupleuri |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102872174A (en) * | 2012-10-26 | 2013-01-16 | 郑州百瑞动物药业有限公司 | Method for preparing bupleurum injection |
-
2008
- 2008-11-14 CN CNA2008101599876A patent/CN101396386A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102872174A (en) * | 2012-10-26 | 2013-01-16 | 郑州百瑞动物药业有限公司 | Method for preparing bupleurum injection |
| CN102872174B (en) * | 2012-10-26 | 2014-04-16 | 郑州百瑞动物药业有限公司 | Method for preparing bupleurum injection |
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Open date: 20090401 |