CN101385807A - Use of rehmannia root whole crude medicine in preventing and treating hyperlipemia and hyperglycaemia - Google Patents
Use of rehmannia root whole crude medicine in preventing and treating hyperlipemia and hyperglycaemia Download PDFInfo
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- CN101385807A CN101385807A CNA2008102119024A CN200810211902A CN101385807A CN 101385807 A CN101385807 A CN 101385807A CN A2008102119024 A CNA2008102119024 A CN A2008102119024A CN 200810211902 A CN200810211902 A CN 200810211902A CN 101385807 A CN101385807 A CN 101385807A
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Abstract
The invention relates to an application of whole rehmannia root powder or an extract thereof to preparing a drug for preventing and/or treating metabolic disorder diseases such as hyperlipemia, hyperglycemia and the like. The invention further relates to a preparation method of the rehmannia root powder or the rehmannia root extract. The rehmannia root powder and the rehmannia root extract can be made into various dosage forms such as capsules, tablets, pills, granules and the like according to the pharmaceutically conventional methods, or made into health foods. The rehmannia root powder and the rehmannia root extract have a simple and practicable preparation method and are suitable for large-scale production.
Description
Technical field
The present invention relates to the application that full medical material of Radix Rehmanniae or Radix Rehmanniae extract are used to prepare the medicine that prevents and/or treats metabolism abnormal diseases such as hyperlipidemia, hyperglycemia.
Background technology
Along with The development in society and economy, the change of life style, the trend that metabolism such as hyperlipidemia, hyperglycemia is significantly increased in worldwide unusually, can cause that thus diseases such as diabetes, hypertension, atherosclerosis and cardiovascular disease become the outstanding problem of harm health of people day by day, so urgent needs is arranged for developing the medicine that can prevent and/or treat metabolism abnormal diseases such as hyperlipidemia, hyperglycemia.
Radix Rehmanniae is the fresh or dry root piece of scrophulariaceae rehmannia glutinosa plant Rehmannia glutinosa Libosch.Again it is divided into Radix Rehmanniae, Radix Rehmanniae (Radix Rehmanniae) and Radix Rehmanniae Preparata in the tcm clinical practice medication.It is said that Radix Rehmanniae is relatively cold, have the effect of clearing away heat and promoting production of body fluid, Radix Rehmanniae can cooling blood for hemostasis, and Radix Rehmanniae Preparata then is used for nourishing YIN and supplementing blood.The old place Huang have enrich blood, the merit of removing heat from blood, accent blood, be the good medicine in the blood.Be usually used in that calentura crimson tongue, interior-heat are quenched one's thirst, the hepatic and renal YIN deficiency, diseases such as osteopyrexia and fever.In recent years, many researchs have been carried out in chemical constituent and the pharmacological action of Radix Rehmanniae both at home and abroad, Radix Rehmanniae has the effect that alleviates glucocorticoid when acting on hormonal system, cardiac contractility ability can be strengthened when acting on cardiovascular system, periphery blood T lymphocyte quantity can be significantly increased when acting on immune system.In addition, Radix Rehmanniae also has as radioprotective, hemostasis, calmness, diuresis, rushes down inferior effect.
On the basis of existing technology, need further the pharmacological effect of full Radix Rehmanniae to be observed, find its single or multiple active princlples with perfect reservation, and manifest the pharmacological effect of Radix Rehmanniae comprehensively its various active princlples.
Summary of the invention
The inventor unexpectedly finds through research, the full medical material of Radix Rehmanniae and have good preventing and/or the unusual effects of metabolism such as treatment hyperlipidemia, hyperglycemia by the ground bloom of its processing, and find that ethanol extraction and catalpol content also have certain unusual effects of metabolism such as hyperlipidemia, hyperglycemia that prevent and/or treat greater than 18% Radix Rehmanniae extract.
Therefore, one aspect of the present invention relates to the application that the full medical material of Radix Rehmanniae (powder) or Radix Rehmanniae extract are used to prepare the medicine that prevents and/or treats metabolism abnormal diseases such as hyperlipidemia, hyperglycemia.
Another aspect of the present invention relates to preparation ground bloom, comprises the method for Radix Rehmanniae, Radix Rehmanniae or Radix Rehmanniae Preparata, characteristics such as that described method has is easy and simple to handle, reliable, good reproducibility, and be suitable for large-scale industrial production.
Another aspect of the present invention relates to the preparation method of Radix Rehmanniae ethanol extraction.
Another aspect of the present invention relates to the preparation method that catalpol content wherein is equal to or greater than the Radix Rehmanniae extract of 18%w/w.
The further aspect of the present invention relates to the various pharmaceutical dosage forms of being made by described ground bloom or Radix Rehmanniae extract.
The further aspect of the present invention relates to the health food of being made by described ground bloom or Radix Rehmanniae extract.
Term used herein " is equal to or greater than the 18%w/w Radix Rehmanniae extract " and is meant that catalpol content is equal to or greater than 18%w/w in the Radix Rehmanniae dry extract.
Specifically, ground of the present invention bloom can prepare by following method: select for use Radix Rehmanniae, Radix Rehmanniae or Radix Rehmanniae Preparata to wash, described Radix Rehmanniae quality of medicinal material all meets " Chinese pharmacopoeia standard.Be dried then, pulverize, promptly get the full medical material powder of Radix Rehmanniae.
More specifically, the full medical material of Radix Rehmanniae is washed,, pulverized 60 to 100 mesh sieves, promptly get the full medical material powder of Radix Rehmanniae at 60-90 ℃ of following vacuum drying.
For the Radix Rehmanniae ethanol extraction, its preparation method comprises the Radix Rehmanniae steaming saturating, pulverizes, and adds triplication 95% ethanol and stirs extraction, and the decompression recycling ethanol extracting solution promptly obtains ethanol extraction.
Greater than for 18% Radix Rehmanniae extract, its preparation method comprises and Radix Rehmanniae is steamed saturating that water is carried, precipitate with ethanol for catalpol content, get supernatant, the reclaim under reduced pressure alcoholic solvent obtains dry extract, after measured, wherein catalpol content approximates 18% w/w, is called 18% Radix Rehmanniae extract in the present invention again.With acetone-water it is processed purification repeatedly, can obtain the wherein higher extract of catalpol content, for example Radix Rehmanniae extract of 20%w/w, 50%w/w and 80%w/w.
Full medical material powder of above-mentioned Radix Rehmanniae or Radix Rehmanniae extract can be made acceptable forms clinically according to formulation method conventional on the pharmaceutics, as capsule, tablet, granule, pill etc.
For example, with regard to the preparation of Radix Rehmanniae arogel wafer of the present invention, can with the present invention of preparing as described above ground bloom directly or with incapsulate shell after one or more capsule usual pharmaceutical adjuvant mixes and make capsule.
With regard to the preparation of ground of the present invention bloom tablet, the present invention's ground bloom and one or more convas tablet adjuvant for preparing as described above can be made tablet as starch, dextrin, lactose, Icing Sugar, calcium sulfate, microcrystalline Cellulose, mannitol, magnesium stearate, gelatine size, mucialga of arabic gummy, methylcellulose, sodium carboxymethyl cellulose, the low hydroxypropyl cellulose that replaces, hydroxypropyl emthylcellulose, polyvidone, sodium alginate, Polyethylene Glycol, cross-linking sodium carboxymethyl cellulose, carboxymethyl starch sodium, Pulvis Talci, micropowder silica gel etc. according to the conventional tablet preparation method.
With regard to the preparation of Radix Rehmanniae powder particles of the present invention agent, the present invention's ground bloom and one or more granule adjuvant commonly used for preparing as described above can be made granule as dextrin, citric acid, sodium citrate etc. according to conventional granulates agent preparation method.
Above-mentioned preparation all can be taked Co
60Ray is according to sterilization.
The specific embodiment
The following example will further specify the present invention, but the present invention will not be construed as limiting.
The preparation of the full medical material powder of embodiment 1 Radix Rehmanniae
The Radix Rehmanniae rhizome is taken the back wash in one week,, pulverized 60 to 100 mesh sieves, promptly get the full medical material powder of Radix Rehmanniae at 60-90 ℃ of following vacuum drying.
The unusual effect of embodiment 2 ground bloom control blood lipid levels
The hyperlipemia rat Preparation of model
Get 50 of 180-220g male Wistar rats, tell 10 and be the normal control group, feed with common rat feed, all the other are fed with special high lipid food (3% cholesterol, 0.2% methylthiouracil, 80% Adeps Sus domestica normal feedstuff) 8 weeks, the preparation hyperlipidemia model is divided into model control group and administration group at random by serum cholesterol content.The feed of freely drinking water, illumination every day 12h, room temperature 18-23 ℃, bedding and padding change once every day.
Medication
The ground bloom is mixed with suspension with 1% Carboxymethyl cellulose sodium, presses 2.5g ground bloom/kg body weight gastric infusion, continuous 5 weeks.
Observation index
Adopt the commercial reagents box, measure the content of triglyceride, cholesterol, high density lipoprotein, low density lipoprotein, LDL in the serum according to operating instruction.
The result
Behind the dosage gastric infusion according to 2.5g ground bloom/kg, the ground bloom is for serum total cholesterol, and triglyceride etc. all have the significant effect of improving, and along with the prolongation of time, this effect is (seeing Table 1-6) further obviously.
Respectively organize blood fat value (n=7) before table 1. administration
| Group | Serum total cholesterol (mmol/L) | Serum high-density LP (mmol/L) | Serum low-density LP (mmol/L) | Serum triglycerides (mmol/L) |
| Normal group | 1.70±0.22 | 0.51±0.15 | 1.23±0.17 | 1.32±0.38 |
| Model group | 3.06±0.50 ## | 0.45±0.21 | 1.63±0.36 ## | 4.59±1.62 ## |
| Ground bloom group | 2.98±0.85 ## | 0.43±0.07 | 1.74±0.31 ## | 3.77±1.19 ## |
Compare with normal group:
#P＜0.05,
##P＜0.01
Table 2. ground bloom is irritated stomach 1 all blood fat values (n=7)
| Group | Serum total cholesterol (mmol/L) | Serum high-density LP (mmol/L) | Serum low-density LP (mmol/L) | Serum triglycerides (mmol/L) |
| Normal group | 1.77±0.27 | 0.63±0.27 | 1.28±0.20 | 1.48±0.47 |
| Model group | 3.62±1.13 ## | 0.40±0.08 # | 2.11±0.88 # | 5.59±1.48 ## |
| Ground bloom group | 2.55±0.55 * | 0.49±0.14 | 1.32±0.34 * | 2.70±0.89 ** |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.0
Table 3. ground bloom is irritated stomach 2 all blood fat values (n=7)
| Group | Serum total cholesterol (mmol/L) | Serum high-density LP (mmol/L) | Serum low-density LP (mmol/L) | Serum triglycerides (mmol/L) |
| Normal group | 1.76±0.36 | 0.71±0.29 | 1.44±0.32 | 1.28±0.16 |
| Model group | 3.76±1.41 ## | 0.42±0.12 # | 2.83±0.69 ## | 5.41±1.25 ## |
| Ground bloom group | 2.40±0.48 * | 0.50±0.18 | 1.44±0.46 ** | 2.84±0.37 ** |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Table 4. ground bloom is irritated stomach 3 all blood fat values (n=7)
| Group | Serum total cholesterol (mmol/L) | Serum high-density LP (mmol/L) | Serum low-density LP (mmol/L) | Serum triglycerides (mmol/L) |
| Normal group | 1.79±0.41 | 0.77±0.35 | 1.41±0.36 | 1.38±0.18 |
| Model group | 4.08±1.49 ## | 0.36±0.09 ## | 2.99±0.61 ## | 6.04±1.70 ## |
| Ground bloom group | 2.61±0.46 * | 0.52±0.21 | 1.78±0.46 ** | 3.64±0.44 ** |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Table 5. ground bloom is irritated stomach 4 all blood fat values (n=7)
| Group | Serum total cholesterol (mmol/L) | Serum high-density LP (mmol/L) | Serum low-density LP (mmol/L) | Serum triglycerides (mmol/L) |
| Normal group | 1.73±0.35 | 0.82±0.36 | 1.41±0.53 | 1.52±0.23 |
| Model group | 4.04±1.38 ## | 0.34±0.13 ## | 3.02±0.44 ## | 5.96±1.50 ## |
| Ground bloom group | 2.49±0.49 * | 0.49±0.20 | 1.98±0.60 ** | 3.90±0.51 ** |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Table 6. ground bloom is irritated stomach 5 all blood fat values (n=7)
| Group | Serum total cholesterol (mmol/L) | Serum high-density LP (mmol/L) | Serum low-density LP (mmol/L) | Serum triglycerides (mmol/L) |
| Normal group | 1.92±0.42 | 0.97±0.34 | 1.58±0.45 | 1.58±0.29 |
| Model group | 4.16±1.52 ## | 0.45±0.12 ## | 3.32±0.43 ## | 6.09±1.68 ## |
| Ground bloom group | 2.76±0.62 * | 0.58±0.24 | 2.24±0.76 ** | 4.20±0.51 ** |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Different with the effect of its extract for showing the present invention ground bloom, the inventor has carried out pharmacological experiment relatively with several Radix Rehmanniae extracts that make in the present invention's ground bloom and the prior art.
The effect of embodiment 3 the present invention ground bloom or its preparation blood fat reducing and blood sugar level
The preparation of hyperlipemia and hyperglycemia rat model
Add hello high lipid food with streptozotocin (STZ) and induce the hyperlipemia and hyperglycemia animal model.Get 70 of 180-220g male Wistar rats, tell 10 as the normal control group, feed with common rat feed, all the other are through tail vein injection 50mg/kg streptozotocin (STZ), select the rat of blood glucose value greater than 11.1mmol/L, according to the blood glucose value random packet, be divided into model control group, bloom group, Radix Rehmanniae extract group and 18% Radix Rehmanniae extract group.Feed with special high lipid food (3% cholesterol, 0.2% methylthiouracil, 80% Adeps Sus domestica, normal feedstuff), the feed of freely drinking water, illumination every day 12h, room temperature 18-23 ℃, bedding and padding change once every day.
Be subjected to the reagent thing
A: ground bloom, its preparation method such as embodiment 1.
B: the Radix Rehmanniae water extract is prepared as follows: Radix Rehmanniae is shredded into about 1cm
3Fritter is used water extraction 2 times, adds 5 times of water gagings at every turn, and soaked overnight heats 80 ℃ and handled 4 hours, is concentrated into every milliliter and contains 1 gram medical material, and is standby.
The C:18% Radix Rehmanniae extract is prepared as follows: Radix Rehmanniae is steamed be cut into 1cm left and right sides small pieces, water extraction 2 times thoroughly.Extracted 2 hours for the first time, add 6 times of water gagings, extracted 1.5 hours for the second time, add 5 times of water gagings.Merge extracted twice liquid, filter, it is about 1.8 to be evaporated to proportion, adds ethanol precipitation, and ethanol content reaches 70%w/w.Get supernatant, decompression recycling ethanol obtains dry extract.The about 18%w/w of catalpol content in this dry extract.
Wherein the content of catalpol can be measured in accordance with the following methods:
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; With acetonitrile-0.1% phosphoric acid solution (1:99) is mobile phase; The detection wavelength is 210nm.Number of theoretical plate calculates by the catalpol peak should be not less than 3000.
It is an amount of that the preparation precision of reference substance solution takes by weighing the catalpol reference substance, adds methanol and make the solution that every 1ml contains 0.3mg, promptly.
The about 0.3g of test sample is got in the preparation of need testing solution, and accurate the title decides, and places the 100ml volumetric flask, adds dissolve with methanol and is diluted to scale, shakes up, and filters with microporous filter membrane (0.45um), promptly.
Accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing inject chromatograph of liquid and measure.
Medication
Be mixed with suspension with 1% Carboxymethyl cellulose sodium, according to the body weight gastric infusion, ground bloom dosage is 2.5g/kg, and Radix Rehmanniae water extract dosage is 2.5g/kg, and 18% Radix Rehmanniae extract dosage is 1g/kg, continuous 4 weeks.
Observation index
Adopt the commercial reagents box, measure triglyceride, cholesterol, high density lipoprotein, low density lipoprotein, LDL content in the serum according to operating instruction.
The result
As shown in table 7, after the modeling, to compare with normal group, each rats in test groups blood glucose value significantly raises (P＜0.01), and the modeling success is described.Secondly, ground bloom group, Radix Rehmanniae water extract group, the 18% Radix Rehmanniae extract group tool effect that has some improvement, but behind the ground bloom group self administration of medication 1 to 4 week all be better than other two groups.
Table 8 to table 11 shows that after the modeling, compare with normal group, each rats in test groups blood fat value significantly changes (P＜0.01), and the modeling success is described.Secondly, ground bloom group, Radix Rehmanniae water extract group, the 18% Radix Rehmanniae extract group tool effect that has some improvement, but the blood sugar lowering and the lipid-lowering effect in 1 to 4 week all are better than other two groups behind the ground bloom group self administration of medication.Especially, for T-CHOL, these two important indicators of triglyceride, ground bloom group effect is particularly outstanding.
Table 7. ground bloom gastric infusion is to the influence (n=7) of hyperglycemia, hyperlipemia rat blood glucose
| Group | Before the administration | 1 week of administration | 2 weeks of administration | 3 weeks of administration | 4 weeks of administration |
| Normal group | 5.03±0.82 | 5.93±1.00 | 5.54±1.06 | 5.84±1.33 | 5.87±0.91 |
| Model group | 19.43±2.02 ## | 20.64±2.68 # | 22.64±3.56 ## | 22.54±4.94 ## | 20.63±4.72 ## |
| Ground bloom group | 19.13±2.06 ## | 16.80±3.34 * | 17.44±2.57 * | 16.40±2.16 * | 14.87±2.53 * |
| The Radix Rehmanniae water extract | 19.44±3.82 ## | 17.84±1.95 * | 18.51±1.36 * | 18.46±1.49 | 16.29±2.00 * |
| 18% Radix Rehmanniae extract | 18.99±3.44 ## | 17.99±2.86 | 18.67±4.47 | 18.83±4.75 | 17.23±5.28 |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05
1 week of table 8. ground bloom gastric infusion is to the influence (n=7) of hyperglycemia, hyperlipemia rat blood fat
| Group | T-CHOL (mmol/L) | High density lipoprotein (mmol/L) | Low density lipoprotein, LDL (mmol/L) | Triglyceride (mmol/L) |
| Normal group | 1.69±0.27 | 0.70±0.28 | 1.15±0.23 | 1.41±0.28 |
| Model group | 2.12±0.52 | 4.78±1.48 | 2.02±0.86 # | 4.78±1.48 ## |
| Ground bloom group | 1.85±0.39 | 0.56±0.21 | 1.63±0.27 | 3.13±1.31 * |
| The Radix Rehmanniae water extract | 2.13±0.88 | 0.55±0.19 | 1.52±0.28 | 3.59±1.67 |
| 18% Radix Rehmanniae extract | 2.08±0.37 | 0.52±0.17 | 1.77±0.36 | 3.82±0.60 |
2 weeks of table 9. ground bloom gastric infusion are to the influence (n=7) of hyperglycemia, hyperlipemia rat blood fat
| Group | T-CHOL (mmol/L) | High density lipoprotein (mmol/L) | Low density lipoprotein, LDL (mmol/L) | Triglyceride |
| Normal group | 1.70±0.25 | 0.72±0.28 | 1.18±0.25 | 1.44±0.28 |
| Model group | 2.27±0.60 # | 0.49±0.07 # | 2.12±0.78 # | 5.01±1.48 ## |
| Ground bloom group | 1.78±0.40 | 0.68±0.23 | 1.64±0.31 | 3.15±1.37 * |
| The Radix Rehmanniae water extract | 2.22±0.87 | 0.62±0.20 | 1.83±0.41 | 3.80±1.60 |
| 18% Radix Rehmanniae extract | 2.37±0.51 | 0.60±0.14 | 2.04±0.38 | 4.14±0.71 |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05
3 weeks of table 10. ground bloom gastric infusion are to the influence (n=7) of hyperglycemia, hyperlipemia rat blood fat
| Group | T-CHOL (mmol/L) | High density lipoprotein (mmol/L) | Low density lipoprotein, LDL (mmol/L) | Triglyceride |
| Normal group | 1.81±0.35 | 0.75±0.28 | 1.33±0.20 | 1.66±0.27 |
| Model group | 2.07±0.63 # | 0.49±0.09 # | 2.39±0.88 # | 5.28±1.57 ## |
| Ground bloom group | 1.88±0.44 * | 0.72±0.19 * | 1.68±0.31 | 3.16±1.29 * |
| The Radix Rehmanniae water extract | 2.25±0.90 | 0.72±0.22 * | 1.94±0.43 | 3.72±1.56 |
| 18% Radix Rehmanniae extract | 2.42±0.57 | 0.55±0.10 | 2.17±0.37 | 4.26±0.71 |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05
4 weeks of table 11. ground bloom gastric infusion are to the influence (n=7) of hyperglycemia, hyperlipemia rat blood fat
| Group | T-CHOL (mmol/L) | High density lipoprotein (mmol/L) | Low density lipoprotein, LDL (mmol/L) | Triglyceride |
| Normal group | 1.88±0.91 | 0.74±0.23 | 1.28±0.26 | 1.65±0.31 |
| Model group | 2.78±0.56 # | 0.50±0.15 # | 2.42±0.92 # | 5.39±1.82 ## |
| Ground bloom group | 1.85±0.38 ** | 0.72±0.23 * | 1.65±0.40 | 3.14±1.36 * |
| The Radix Rehmanniae water extract | 2.16±0.79 | 0.69±0.21 * | 1.89±0.37 | 3.63±1.40 |
| 18% Radix Rehmanniae extract | 2.40±0.61 | 0.56±0.12 | 2.14±0.72 | 4.22±0.72 |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
The above results shows that aspect blood fat reducing and blood sugar lowering, ground bloom, Radix Rehmanniae water extract group and 18% Radix Rehmanniae extract are all effective, with the best results of ground bloom.
Embodiment 4 the present invention ground bloom or its preparation are to the effect of abnormal liver function
Adopt the commercial reagents box,, measured the content of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) in the foregoing description 6 animal serums according to operating instruction.
,, compare with normal group after the modeling to shown in the table 15 as table 12, each rats in test groups liver function changes significantly (P＜0.01), and the molding success is described.Secondly, the liver function effect of improving of ground bloom group and 18% Radix Rehmanniae extract group is better than Radix Rehmanniae water extract group.
Serum transaminase value (n=7) in table 12. ground bloom gastric infusion 1 all hyperglycemia, the hyperlipemia rat
| Group | Glutamate pyruvate transaminase ALT (u/L) | Glutamic oxaloacetic transaminase, GOT AST (u/L) |
| Normal group | 101.14±23.35 | 47.29±8.67 |
| Model group | 145.29±19.13 ## | 53.43±16.32 |
| Ground bloom group | 120.43±11.39 * | 46.14±11.39 |
| The Radix Rehmanniae water extract | 127.14±19.98 * | 51.43±20.27 |
| 18% Radix Rehmanniae extract | 103.43±29.26 | 41.43±0.79 |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Serum transaminase value (n=7) in table 13. ground bloom gastric infusion 2 all hyperglycemia, the hyperlipemia rat
| Group | Glutamate pyruvate transaminase ALT (u/L) | Glutamic oxaloacetic transaminase, GOT AST (u/L) |
| Normal group | 105.14±23.40 | 51.14±8.97 |
| Model group | 148.29±18.44 ## | 58.00±17.28 |
| Ground bloom group | 122.57±15.0 * | 47.71±12.85 |
| Radix Rehmanniae water extract | 134.29±23.14 * | 51.14±18.04 |
| 18% Radix Rehmanniae extract | 106.14±36.03 | 45.14±6.41 |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Serum transaminase value (n=7) in table 14. ground bloom gastric infusion 3 all hyperglycemia, the hyperlipemia rat
| Group | Glutamate pyruvate transaminase ALT (u/L) | Glutamic oxaloacetic transaminase, GOT AST (u/L) |
| Normal group | 107.43±23.76 | 51.86±10.70 |
| Model group | 148.86±15.81 ## | 57.57±14.71 |
| Ground bloom group | 122.21±21.38 * | 48.29±13.61 |
| The Radix Rehmanniae water extract | 129.71±23.94 | 51.86±18.19 |
| 18% Radix Rehmanniae extract | 117.86±35.21 | 47.43±8.14 |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05
Serum transaminase value (n=7) in table 15. ground bloom gastric infusion 4 all hyperglycemia, the hyperlipemia rat
| Group | Glutamate pyruvate transaminase ALT (u/L) | Glutamic oxaloacetic transaminase, GOT AST (u/L) |
| Normal group | 101.29±21.89 | 52.00±9.87 |
| Model group | 145.43±17.97 ## | 55.57±13.96 |
| Ground bloom group | 116.86±27.15 * | 47.29±16.44 |
| The Radix Rehmanniae water extract | 131.00±30.68 | 54.14±19.32 |
| 18% Radix Rehmanniae extract | 114.57±34.82 | 52.14±10.37 |
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05
Therefore, according to above-mentioned effect and data of safety, can judge that ground bloom, 18% Radix Rehmanniae extract have more actual application value.
The hypoglycemic effect of embodiment 5 different purity Radix Rehmanniae extracts
As mentioned above, 18% Radix Rehmanniae extract has the certain control hyperlipidemia and the effect of hyperglycemia, with acetone-water it is processed purification repeatedly, can obtain the higher extract of catalpol content, the Radix Rehmanniae extract of 20%w/w, 50%w/w and 80%w/w for example, and can keep or even improve effect.(as embodiment 6) shows in view of above-mentioned experiment, and Radix Rehmanniae has the effect of linking for hyperlipidemia or hyperglycemia, therefore observe the two one of can understand the effect of its control hyperlipidemia, hyperglycemia.
Alloxan causes the preparation of mouse blood sugar elevation model
Get 80 healthy KM mices, ♂ tells 8 at random as the normal control group.All the other mices behind fasting 16h, the freshly prepared 2% alloxan normal saline solution of tail vein fast injection, dosage is 60mg/kg.Normal control group mice behind fasting 16h in tail vein injection equal-volume normal saline.Above-mentioned mice is got blood in the tail point and measures blood glucose value with blood glucose meter through after the free diet of 72h, select blood glucose value greater than the mice of 16.7mmol/L as the blood sugar increasing mice.
The experiment grouping
The mice of getting 40 modeling successes is divided into 5 groups by the blood glucose value stratified random, and 8 every group, i.e. model control group, 80% purity Radix Rehmanniae extract, 50% purity Radix Rehmanniae extract, 20% purity Radix Rehmanniae extract.Animal gives the standard mouse feed, free illumination every day 12h, and room temperature 18-23 ℃, bedding and padding change once every day.
Medication
The Radix Rehmanniae extract of different purity serves as unified standard dosage conversion administration with the catalpol of 100mg/kg, is mixed with medicinal liquid with normal saline, according to the body weight gastric infusion, and continuous 3 weeks.
Observation index
The mice serum blood glucose value.Measure according to GLUCOCARD II blood glucose meter operating instruction.
The result
As shown in Table 16, after the modeling, compare with normal group, each rats in test groups blood glucose value significantly raises (P＜0.01), and the modeling success is described.Secondly, the blood glucose value of Radix Rehmanniae extract orally give 2 Zhou Junke of 80%, 50% and 20% purity remarkable reduction hyperglycemia mice in various degree, and have the purity dependence.Therefore can determine promptly to have the effect of control hyperlipidemia, hyperglycemia greater than 18% Radix Rehmanniae extract.
The Radix Rehmanniae extract of table 16. different purity is to the influence (n=8) of hyperglycemia mice hyperglycemia
Effects such as embodiment 6. various dose Radix Rehmanniae ethanol extraction blood sugar lowering
Alloxan causes the mice diabetes, the blood sugar increasing Preparation of model as above, the test be divided into 6 groups at random, every group of 7 mices.Medication by various dose Radix Rehmanniae ethanol extraction, is mixed with medicinal liquid with normal saline, according to the body weight gastric infusion, and continuous 3 weeks.
The result
As table 17,18 show, after the modeling, compare with normal group, each test group mice serum index measurement value significantly changes, and the modeling success is described.And the Radix Rehmanniae ethanol extraction has a significant effect, and effective dosage ranges is quite wide.
Table 17. Radix Rehmanniae ethanol extraction to the influence of mice hyperglycemia (x ± s, n=7)
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Table 18. Radix Rehmanniae ethanol extraction to mice serum lipid and transaminase's influence (x ± s, n=7)
Compare with normal group:
#P＜0.05,
##P＜0.01; Compare with model group:
*P＜0.05,
*P＜0.01
Claims (11)
1. the full medical material of the Radix Rehmanniae application that is used to prepare the medicine that prevents and/or treats metabolism abnormal diseases such as hyperlipidemia, hyperglycemia.
2. the purposes of claim 1, the full medical material of wherein said Radix Rehmanniae are the ground bloom.
3. the purposes of claim 1, the full medical material of wherein said Radix Rehmanniae is a Radix Rehmanniae extract, and described Radix Rehmanniae extract is that catalpol content is equal to or greater than 18%w/w.
4. each purposes of claim 1-3, wherein said Radix Rehmanniae is Radix Rehmanniae, Radix Rehmanniae or Radix Rehmanniae Preparata.
5. each purposes of claim 1-3, wherein said Radix Rehmanniae is a Radix Rehmanniae.
6. the preparation method of bloom, described method comprises selects for use Radix Rehmanniae, Radix Rehmanniae or Radix Rehmanniae Preparata to wash, and is dried then, pulverizes, and promptly gets the full medical material powder of Radix Rehmanniae.
7. the method for claim 6, described method comprise Radix Rehmanniae to be pulverized and also crosses 60 to 100 mesh sieves at 60-90 ℃ of following vacuum drying.
8. the preparation method of Radix Rehmanniae extract, described method comprise steams Radix Rehmanniae saturating, and water is carried, precipitate with ethanol, get supernatant, the reclaim under reduced pressure alcoholic solvent obtains dry extract, wherein catalpol content approximates 18%w/w, is further purified to obtain the higher extract of catalpol content, perhaps
Radix Rehmanniae is steamed saturating, pulverize, add triplication 95% ethanol and stir and extract, the decompression recycling ethanol extracting solution promptly obtains the ethanol extraction of Radix Rehmanniae.
9. rehmannia preparation, it contains according to the ground bloom of claim 6 or 7 described methods preparations or according to the Radix Rehmanniae extract of the described method preparation of claim 8, and one or more pharmaceuticss adjuvant commonly used.
10. the described rehmannia preparation of claim 9 is characterized in that said preparation is tablet, capsule, granule or pill.
11. health food, it contains according to the ground bloom of claim 6 or 7 described method preparations or the Radix Rehmanniae extract for preparing according to the described method of claim 8, and one or more general food additives.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008102119024A CN101385807A (en) | 2007-09-14 | 2008-09-09 | Use of rehmannia root whole crude medicine in preventing and treating hyperlipemia and hyperglycaemia |
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| CN200710153896.7 | 2007-09-14 | ||
| CN200710153896 | 2007-09-14 | ||
| CNA2008102119024A CN101385807A (en) | 2007-09-14 | 2008-09-09 | Use of rehmannia root whole crude medicine in preventing and treating hyperlipemia and hyperglycaemia |
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| CN115919949A (en) * | 2022-12-09 | 2023-04-07 | 河南省纳普生物技术有限公司 | Rehmannia functional factor extract and application thereof in preparation of anti-oxidation and/or hypoglycemic drugs |
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| CN115919949A (en) * | 2022-12-09 | 2023-04-07 | 河南省纳普生物技术有限公司 | Rehmannia functional factor extract and application thereof in preparation of anti-oxidation and/or hypoglycemic drugs |
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Application publication date: 20090318 |