CN101377023A - Method for producing nano-scale fiber by wheat mucedin - Google Patents
Method for producing nano-scale fiber by wheat mucedin Download PDFInfo
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- CN101377023A CN101377023A CNA2007101212248A CN200710121224A CN101377023A CN 101377023 A CN101377023 A CN 101377023A CN A2007101212248 A CNA2007101212248 A CN A2007101212248A CN 200710121224 A CN200710121224 A CN 200710121224A CN 101377023 A CN101377023 A CN 101377023A
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Abstract
The invention provides a method for producing nano fiber with glutenin and gliadin through electrostatic spinning. In the method, whole meal flour serves as the raw material; first, the glutenin and the gliadin are respectively extracted; and then polymer serves as the carrier of the glutenin and the gliadin, and the electrostatic spinning is conducted. The spinning voltage is 10KV to 30KV, the distance from a spray nozzle to a collecting board is 10cm to 25cm, and the flowing speed of spinning solution is 0.5ml/h to 1.5ml/h. Analysis to the characteristics of composite fiber obtained by blended spinning shows that the fiber after blended spinning meets the standards of nano fiber; the structure is more even; the air permeability and water absorption are better; the elasticity and extensibility are strengthened.
Description
Technical field
The invention belongs to deep processing of farm products and nanofiber spinning field, particularly utilize mucedin and high polymer blending to produce the method for nano-scale fiber.
Background technology
Wheat gluten protein claims vital wheat gluten again, is a kind of accessory substance that obtains when producing wheaten starch, contains 75%～85% protein.On chemical composition, wheat gluten protein is mainly less by relative molecular mass, be spherical, have better ductile gliadin and relative molecular mass is big, be fibrous, have than the glutenin of strong elasticity and form.According to statistics, the annual production of global Gluten is approximately 500,000 tons.As the plant protein source of high-quality, Gluten itself has very high nutritive value.Gluten is mainly used in food industry and feed industry at present, for example as green flour gluten fortifier or flour-dough improver, is used for the production of products such as flour, instant noodles, ham sausage, high-grade aquatic products and feed for pet; Also can be used as water retention agent is applied in the meat product.
In recent years, the output of gluten continues to increase, and its traditional market hastens towards saturation, and therefore exploring the new approach that utilizes of mucedin becomes certainty.Amino acid composition analysis shows that mucedin contains more glutamine, cysteine, nonpolar amino acid.Glutenin and gliadin be as the main component of mucedin, mainly by amino acid inner or between hydrogen bond, effects such as disulfide bond and static combine.Glutenin has unique supersecondary structure, and the βZhe Die structure at its middle part is similar to the supersecondary structure of spider silk, has the self-condition of silk, and this has given gluten many peculiar properties: viscoplasticity, extensibility, film forming etc.
Electrostatic spinning technique is to obtain one of most important method of nano-scale fiber at present.The electrospinning fibre diameter of making by electrostatic spinning generally in tens nanometers between the hundreds of nanometers, are a kind of porous fibres by these fibroplastic nonwoven fabric with nanometer micropore, therefore very big specific area is arranged, multiple potential use is arranged.
Prepare the characteristics of nanofiber technology at wheat gluten protein and electrostatic spinning, we have researched and developed and have utilized electrospinning process, by the carrier of high polymer as wheat glutenin and gliadin, prepare the method for nano-scale fiber.For the dark Application and Development of wheat gluten protein in the non-food stuff industry provides new approach.
Summary of the invention
The present invention relates to a kind of electrospinning process that passes through, the method for utilizing the mixed electrostatic spinning of wheat glutenin, gliadin and high polymer to produce the novel nano fiber respectively.
Design of the present invention is as follows: wheat gluten protein mainly is made up of glutenin and gliadin, and the gliadin molecule is spherical, and molecular weight less (25000～100000) have extensibility, but elasticity is little; The glutenin molecule is fibrous, and molecular weight is big (more than 100000), and elasticity is stronger, but extensibility is little.Glutenin and gliadin acting in conjunction make wheat gluten have unique viscoelasticity.Utilize the characteristics of wheat gluten protein,, itself and high polymer are carried out blending produce the novel nano fiber, improve the extensibility and the elasticity of nanofiber by electrospinning process.Thereby opened up new way for the deep processing of wheat flour, improved the added value of wheat gluten protein.
Method and step that the present invention utilizes wheat gluten protein to produce nano-scale fiber comprise:
1, the extraction of wheat gluten protein;
2, the extraction of glutenin;
3, the extraction of gliadin;
4, the mixing electrostatic spinning of glutenin and high polymer, the preparation nano-scale fiber;
5, the mixing electrostatic spinning of gliadin and high polymer, the preparation nano-scale fiber.
Wherein the extraction of wheat gluten protein comprises in 1:
Wheat flour is mixed according to solid-to-liquid ratio 1:1～3 with water, rubs into dough, add then 3～8 times water clean, obtain water-fast asperities muscle albumen.
The method of extracting glutenin in 2 is:
Reagent is extracted in preparation: 0.05～0.1 mol acetate, 2.5～5%SDS, 1～5 milli protein protective agent that rubs/rise.Is that 1:10～20 mixes with extracting reagent according to solid-to-liquid ratio with the asperities muscle albumen that obtains in 2, and extracting temperature is 20～35 ℃, and concussion is extracted 2-4 time, total 1～6 hour extraction time, merging extract.Regulate pH value to 6～7, in the supercentrifuge of 10000～15000rpm centrifugal 10～25 minutes then, precipitate and be glutenin.
The method of extracting gliadin in 3 is:
Reagent is extracted in preparation: 50-70% ethanolic solution, 1～5 milli protein protective agent that rubs/rise.Is that 1:10～30 mixes with extracting reagent according to solid-to-liquid ratio with the asperities muscle albumen that obtains in 2, and extracting temperature is 20～35 ℃, and concussion is extracted 2～5 times, total 2～6 hours extraction times, merging extract.The content to 5 of ethanol in the regulator solution～20%, centrifugal extraction 10～25 minutes in the supercentrifuge of 10000～15000rpm then, precipitation is gliadin.
The mixing electrospinning process of glutenin and high polymer comprises in 4:
The high polymeric solution of preparation 10-20%, solvent is 0.05～0.1 mol acetate, 2.5～5%SDS.The glutenin that obtains in 2 is mixed with high polymeric solution, and the concentration that makes glutenin is 10～25%.Mixed liquor is joined in the electrostatic spinning apparatus, voltage 15～25KV, shower nozzle is 10～20 centimetres to the distance of collecting board.The flow velocity of spinning solution is controlled at 0.5～1.5 milliliter/hour.
The mixing electrospinning process of gliadin and high polymer comprises in 5:
The high polymeric solution of preparation 10-25%, solvent is 40～80% ethanolic solutions.The gliadin solution that obtains in 3 is mixed with high polymeric solution, and the concentration that makes gliadin is 10～30%.Mixed liquor is joined in the electrostatic spinning apparatus, voltage 10～30KV, shower nozzle is 10～25 centimetres to the distance of collecting board.The flow velocity of spinning solution is controlled at 0.5～1.5 milliliter/hour.
The present invention has the following advantages:
Accessory substance when 1) mucedin is as production starch, output is abundant.Make full use of the design feature of mucedin, this method has proposed wheat gluten protein first and high polymer carries out the new technology that nanofiber is produced in blending.This technology can improve the added value of wheat flour, and this has opened up new direction for making full use of mucedin, has broad application prospects.
2) mucedin and high polymer carry out the fiber that electrostatic spinning obtains and reach nanoscale, compare with the fiber that pure high polymer spinning obtains, and the fiber quality that glutenin and high polymer blending make is more even, and elasticity strengthens, and gas permeability is better, and water absorbing properties is stronger; The fiber extensibility that gliadin and high polymer blending make, water absorbing properties and gas permeability strengthen.
3) wheat gluten protein that is utilized is the accessory substance when producing starch, and output is abundant.This has guaranteed low cost and sufficient raw material supply that nanofiber is produced.
Description of drawings
Fig. 1 is for to carry out the scanning electron microscope diagram that spinning obtains fiber with pure polyvinyl alcohol as high polymer.
PVA (* 500): amplify 500 times of Electronic Speculum figure; PVA (* 5,000): amplify 5000 times of Electronic Speculum figure; PVA (* 15,000): amplify 15,000 times of Electronic Speculum figure.
Fig. 2 obtains the scanning electron microscope diagram of fiber for polyvinyl alcohol and glutenin blending.
PVA (* 500): amplify 500 times of Electronic Speculum figure; PVA (* 5,000): amplify 5000 times of Electronic Speculum figure; PVA (* 15,000): amplify 15,000 times of Electronic Speculum figure.
The specific embodiment
Example 1: glutenin and polyvinyl alcohol (PVA) blending prepares nano-scale fiber
The extraction of mucedin: wheat flour is mixed according to solid-to-liquid ratio 1:2 with water, rubs into dough, add then 5 times water clean, obtain water-fast asperities muscle albumen.
The extraction of glutenin: reagent is extracted in preparation: 0.08 mol acetate, 3%SDS, the 2 millis phenylmethylsulfonyl fluoride that rubs/rise.Is that 1:15 mix with extracting reagent according to solid-to-liquid ratio with asperities muscle albumen, and extracting temperature is 25 ℃, and concussion is extracted 3 times, total 4 hours extraction times, merges extract.Regulate pH value to 6.5, in the supercentrifuge of 12000rpm centrifugal 15 minutes then, precipitate and be glutenin.
The poly-vinyl alcohol solution of preparation 15%, solvent is 0.08 mol acetate, 3%SDS.The glutenin that obtains is mixed with high polymeric solution, and the concentration that makes glutenin is 12%.Mixed liquor is joined in the electrostatic spinning apparatus, voltage 20KV, shower nozzle is 15 centimetres to the distance of collecting board.The flow velocity of spinning solution is controlled at 0.8 milliliter/hour.
Example 2: gliadin and polyvinylpyrrolidone blending prepare nano-scale fiber
The extracting method of mucedin such as example 1.
The extraction of gliadin: reagent is extracted in preparation: 60% ethanolic solution, the 2.5 millis phenylmethylsulfonyl fluoride that rubs/rise.Is that 1:12 mix with extracting reagent according to solid-to-liquid ratio with gluten, and extracting temperature is 25 ℃, and concussion is extracted 4 times, total 2.5 hours extraction times, merges extract.The content to 12% of ethanol in the regulator solution, centrifugal extraction 15 minutes in the supercentrifuge of 11000rpm then, precipitation is gliadin.
Polyvinylpyrrolidone is dissolved in 70% ethanol, and the preparation mass fraction is 15% electrospinning silk solution.Gliadin solution is mixed with the polyvinylpyrrolidone ethanolic solution, and the concentration that makes gliadin is 18%.Mixed liquor is joined in the electrostatic spinning apparatus, voltage 18KV, shower nozzle is 20 centimetres to the distance of collecting board.The flow velocity of spinning solution is controlled at 1.2 milliliters/hour.
Example 3: glutenin and polyvinylpyrrolidone blending prepare nano-scale fiber
The extracting method of mucedin and glutenin such as example 1.
The polyvinylpyrrolidonesolution solution of preparation 12%, solvent is 0.06 mol acetate, 3%SDS.Glutenin solution is mixed with polyvinylpyrrolidonesolution solution, and the concentration that makes glutenin is 15%.Mixed liquor is joined in the electrostatic spinning apparatus, voltage 22KV, shower nozzle is 14 centimetres to the distance of collecting board.The flow velocity of spinning solution is controlled at 0.9 milliliter/hour.
Claims (6)
1. utilize wheat gluten protein to produce the method for nano-scale fiber, it is characterized in that may further comprise the steps: the 1) extraction of wheat gluten protein; 2) extraction of glutenin; 3) extraction of gliadin; 4) the mixing electrostatic spinning of glutenin and high polymer, the preparation nano-scale fiber; 5) the mixing electrostatic spinning of gliadin and high polymer, the preparation nano-scale fiber.
2. according to claim 1 1) leaching process of wheat gluten protein is characterized as: wheat flour is mixed according to solid-to-liquid ratio 1:1～3 with water, rub into dough, the water that adds 3～8 times then cleans, and obtains water-fast asperities muscle albumen.
3. according to claim 1 2) leaching process of wheat glutenin is characterized as: reagent is extracted in preparation: 0.05～0.1 mol acetate, 2.5～5% dodecyl sodium sulfates (SDS), 1～5 milli protein protective agent that rubs/rise; Is that 1:10～20 mixes with extracting reagent according to solid-to-liquid ratio with the asperities muscle albumen that obtains in 2, and extracting temperature is 20～35 ℃, and concussion is extracted 2-4 time, total 1～6 hour extraction time, merging extract; Regulate pH value to 6～7, in 10000～15000 rev/mins supercentrifuge centrifugal 10～25 minutes then, precipitate and be glutenin.
4. according to claim 1 3) leaching process of wheat gliadin is characterized as: reagent is extracted in preparation: 50-70% ethanolic solution, 1～5 milli SDS that rubs/rise; Is that 1:10～30 mixes with extracting reagent according to solid-to-liquid ratio with the asperities muscle albumen that obtains in 2, and extracting temperature is 20～35 ℃, and concussion is extracted 2～5 times, total 2～6 hours extraction times, merging extract; The content to 5 of ethanol in the regulator solution～20%, in 10000～15000 rev/mins supercentrifuge centrifugal 10～25 minutes then, precipitation was gliadin.
5. according to claim 1 4) being characterized as of the mixing electrostatic spinning of glutenin and high polymer: the high polymeric solution of preparation 10-20%, solvent is 0.05～0.1 mol acetate, 2.5～5%SDS, the glutenin that obtains in 3 is mixed with high polymeric solution, and the concentration that makes glutenin is 10～25%; Mixed liquor is joined in the electrostatic spinning apparatus, voltage 15～25KV, shower nozzle is 10～20 centimetres to the distance of collecting board; The flow velocity of spinning solution is controlled at 0.5～1.5 milliliter/hour.
6. according to claim 1 5) being characterized as of the mixing electrostatic spinning of gliadin and high polymer: the high polymeric solution of preparation 10-25%, solvent is 40～80% ethanolic solutions; The gliadin solution that obtains in 4 is mixed with high polymeric solution, and the concentration that makes gliadin is 10～30%; Mixed liquor is joined in the electrostatic spinning apparatus, voltage 10～30KV, shower nozzle is 10～25 centimetres to the distance of collecting board; The flow velocity of spinning solution is controlled at 0.5～1.5 milliliter/hour.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634860A (en) * | 2011-12-09 | 2012-08-15 | 新乡市飞跃科技发展有限公司 | Wheat protein fiber and manufacturing method thereof |
| CN104365990A (en) * | 2014-11-20 | 2015-02-25 | 华中农业大学 | Wheat alcohol-soluble protein and glutelin as well as preparation method and application thereof |
| CN104532380A (en) * | 2014-12-30 | 2015-04-22 | 江南大学 | Preparation method for high-strength wheat protein fiber |
| CN109853078A (en) * | 2018-12-25 | 2019-06-07 | 杭州优标科技有限公司 | A kind of wheat gluten regenerated celulose fibre and preparation method thereof |
| CN111349977A (en) * | 2020-05-06 | 2020-06-30 | 浙江大学 | Wheat gluten protein nano-fiber and preparation method and application thereof |
-
2007
- 2007-08-31 CN CN2007101212248A patent/CN101377023B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634860A (en) * | 2011-12-09 | 2012-08-15 | 新乡市飞跃科技发展有限公司 | Wheat protein fiber and manufacturing method thereof |
| CN102634860B (en) * | 2011-12-09 | 2014-03-05 | 新乡市飞跃科技发展有限公司 | Wheat protein fiber and manufacturing method thereof |
| CN104365990A (en) * | 2014-11-20 | 2015-02-25 | 华中农业大学 | Wheat alcohol-soluble protein and glutelin as well as preparation method and application thereof |
| CN104365990B (en) * | 2014-11-20 | 2017-10-17 | 华中农业大学 | A kind of wheat gliadin and glutelin and preparation method and application |
| CN104532380A (en) * | 2014-12-30 | 2015-04-22 | 江南大学 | Preparation method for high-strength wheat protein fiber |
| CN109853078A (en) * | 2018-12-25 | 2019-06-07 | 杭州优标科技有限公司 | A kind of wheat gluten regenerated celulose fibre and preparation method thereof |
| CN109853078B (en) * | 2018-12-25 | 2021-08-06 | 杭州优标科技有限公司 | Wheat protein regenerated cellulose fiber and preparation method thereof |
| CN111349977A (en) * | 2020-05-06 | 2020-06-30 | 浙江大学 | Wheat gluten protein nano-fiber and preparation method and application thereof |
| WO2021223511A1 (en) * | 2020-05-06 | 2021-11-11 | 浙江大学 | Wheat gluten protein nanofiber, preparation method therefor, and application thereof |
| US20220349092A1 (en) * | 2020-05-06 | 2022-11-03 | Zhejiang University | Wheat gluten nanofiber, method for preparing the same and application thereof |
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| CN101377023B (en) | 2010-12-08 |
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