CN111778635B - Preparation method of peanut protein-polyurethane nanofiber membrane - Google Patents
Preparation method of peanut protein-polyurethane nanofiber membrane Download PDFInfo
- Publication number
- CN111778635B CN111778635B CN202010672723.1A CN202010672723A CN111778635B CN 111778635 B CN111778635 B CN 111778635B CN 202010672723 A CN202010672723 A CN 202010672723A CN 111778635 B CN111778635 B CN 111778635B
- Authority
- CN
- China
- Prior art keywords
- polyurethane
- peanut protein
- nanofiber membrane
- peanut
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
- D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/02—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from cellulose, cellulose derivatives, or proteins
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F8/00—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof
- D01F8/04—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers
- D01F8/16—Conjugated, i.e. bi- or multicomponent, artificial filaments or the like; Manufacture thereof from synthetic polymers with at least one other macromolecular compound obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds as constituent
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4382—Stretched reticular film fibres; Composite fibres; Mixed fibres; Ultrafine fibres; Fibres for artificial leather
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H3/00—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length
- D04H3/005—Synthetic yarns or filaments
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H3/00—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length
- D04H3/02—Non-woven fabrics formed wholly or mainly of yarns or like filamentary material of substantial length characterised by the method of forming fleeces or layers, e.g. reorientation of yarns or filaments
Landscapes
- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Mechanical Engineering (AREA)
- Artificial Filaments (AREA)
Abstract
The invention discloses a preparation method of a peanut protein-polyurethane nanofiber membrane, which comprises the following steps: dissolving polyurethane in a solvent, and stirring until the polyurethane is completely dissolved to obtain a polyurethane solution; dissolving peanut protein in the polyurethane solution, and stirring until the peanut protein and the polyurethane solution are uniformly mixed to obtain a polyurethane-peanut protein solution; and (3) performing electrostatic spinning by adopting a polyurethane-peanut protein solution to prepare the peanut protein-polyurethane nanofiber membrane. On one hand, the peanut protein-polyurethane nanofiber membrane prepared by the invention not only keeps the good biological activity of peanut protein, but also has good toughness and mechanical strength of polyurethane; on the other hand, the plant protein nanofiber membrane is developed by taking the defatted peanut powder as the raw material, so that peanut protein resources such as peanut meal and the like are effectively utilized, the peanut is promoted to be further converted into a high-added-value product, and the plant protein nanofiber membrane has good economic and environmental benefits.
Description
Technical Field
The invention relates to the technical field of biomedical materials, in particular to a preparation method of a peanut protein-polyurethane nanofiber membrane, and specifically relates to an electrostatic spinning preparation method.
Background
Only about 15% of peanuts in the countries of the United states, European Union and the like are used for extracting the peanut oil, and byproducts of the peanuts used for extracting the oil are reasonably and comprehensively utilized. Most of peanuts produced in the countries of the United states, European Union and the like are used for processing serial foods and health care products by utilizing the technology of separating components such as lipids, proteins and the like by cold pressing, and the additional value of the peanuts is improved. Compared with America and European Union, although China has abundant peanut resources, the method lags behind aspects such as processing equipment, scientific research investment, technical innovation and the like of peanuts, and starts to be late in comprehensive utilization of the peanuts. In China, more than 60% of peanuts are used for oil extraction, the original nutritional value of the peanuts is lost due to the denaturation of protein in the peanut meal left after hot oil extraction of the peanuts, and the research and application of the peanut meal after oil preparation are less, so the peanut meal is usually used as feed or fertilizer, and the comprehensive utilization efficiency of the peanuts is extremely low.
The functional characteristics of the peanut protein mainly comprise water retention, solubility, viscosity, gelling property, emulsifying stability, foaming property, foam stability and the like. These functional properties are related not only to the physical properties of the protein, such as its amino acid composition, molecular size, and structural morphology, but also to the environmental conditions (e.g., temperature, pH, ionization intensity, etc.) in which other protein-interacting food components (e.g., water, ions, lipids, etc.) are located. The isolated peanut protein is a protein which has no thermal denaturation and has the protein content of more than 90 percent and is refined by a low-temperature peanut protein preparation technology. The extraction method of the peanut protein isolate mainly comprises an alkali-soluble acid precipitation method, an ultrafiltration membrane method and an aqueous method, wherein the alkali-soluble acid precipitation method is simple and convenient to operate and has high extraction rate. The protein content of the peanut protein isolate is higher, generally more than 90%. The peanut protein isolate has all the excellent characteristics of peanut protein, and has higher biocompatibility and better biodegradability. The peanut protein isolate is reproducible, has wide sources, good nutrition, low price, good processability, good film forming property and air barrier property, and the material prepared by the peanut protein isolate has good biocompatibility and degradability.
However, because the molecular weight of peanut protein is low, the membrane material made of a single peanut protein material is generally brittle and has low mechanical strength, so that the applicability of the peanut protein material is limited. Therefore, at present, most peanut protein-based biomaterials are often prepared by compounding peanut protein with natural polymers or artificial polymers, so that the defect of poor mechanical property of single peanut protein, such as silk fibroin, can be overcome.
The electrostatic spinning technology is that a high-voltage electric field environment is utilized to enable polymer spinning solution to form an electrified jet flow, the jet flow is elongated under the action of an electric field, a solvent is volatilized, and finally nano fibers in a certain shape are formed on a receiving device. In recent ten years, the technology becomes one of effective ways for preparing nanofiber materials, and the obtained nanofiber has high porosity and controllable form and is widely applied to the fields of medical dressings, tissue engineering scaffolds and the like.
Because the effect of the pure-spun peanut protein nanofiber is not ideal, some additional polymers, such as polyurethane, polyvinyl alcohol, polylactic acid and the like, must be introduced to increase the spinnability of the pure-spun peanut protein nanofiber. Medical polyurethane is a commercial synthetic polymer material, has the advantages of good flexibility, high mechanical strength, air permeability, water resistance and the like, and is widely applied to the fields of wound care, cardiology, plastic surgery, vascular department and the like at present. In the prior art, documents using peanut protein as a nanofiber membrane have few relevant reports, and the preparation of the nanofiber membrane by using peanut protein and polyurethane is not related.
Disclosure of Invention
In order to overcome the problems in the prior art, the invention provides a preparation method of a peanut protein-polyurethane nanofiber membrane, which is based on the fact that the water solubility of peanut protein is poor, a solvent capable of dissolving peanut protein and polyurethane is selected, and conditions of all the steps are researched and optimized, so that the prepared peanut protein-polyurethane nanofiber membrane not only keeps good bioactivity of peanut protein, but also has good toughness and mechanical strength of polyurethane, and is expected to be applied to the fields of medical dressings and the like.
In order to achieve the purpose, the invention adopts the following technical scheme:
the first aspect of the present invention provides a method for preparing a peanut protein-polyurethane nanofiber membrane, comprising the following steps:
step A: dissolving polyurethane in a solvent, and stirring until the polyurethane is completely dissolved to obtain a polyurethane solution; dissolving peanut protein in the polyurethane solution, and stirring until the peanut protein and the polyurethane solution are uniformly mixed to obtain a polyurethane-peanut protein solution;
and B: and D, performing electrostatic spinning by using the polyurethane-peanut protein solution in the step A to prepare the peanut protein-polyurethane nanofiber membrane.
In order to further optimize the preparation method, the technical measures adopted by the invention also comprise the following steps:
further, the molecular weight of the peanut protein is 14-66 kDa; the average molecular weight of the polyurethane is 8-12 ten thousand, and more preferably 10 ten thousand. It will be appreciated that the molecular weights of the peanut proteins and polyurethanes are within the desired spinnable range for electrospinning, and the above mentioned molecular weights are only exemplary preferred choices.
Further, in the step A, the mass fraction of the polyurethane solution is 1% -5%.
Further, in the step A, the mass ratio of the polyurethane to the peanut protein is 1:5-5: 1.
Further, the polyurethane may be medical grade, food grade, industrial grade, extrusion grade, and the like, and more preferably PU (1180a10), extrusion grade.
Further, the solvent is one or more of hexafluoroisopropanol, N-dimethylformamide, N-dimethylacetamide, N-methylpyrrolidone, dichloromethane, dichloroethane, chloroform, tetrahydrofuran, formic acid, dimethyl sulfoxide (DMSO), acetic acid and ethanol; more preferably hexafluoroisopropanol. It is understood that the solvent may be any suitable solvent in the art that can dissolve both peanut protein and polyurethane, and that the dissolved solution may be electrospun.
Further, before the step A, the method also comprises the step a: preparing peanut protein; wherein the step a comprises: crushing low-temperature peanut meal obtained by squeezing peanuts at a low temperature, degreasing, and obtaining peanut protein by an alkali-soluble acid precipitation method.
Further, the degreasing treatment is performed using a low-polarity solvent such as petroleum ether or n-hexane, and more preferably n-hexane.
Further, the step a specifically includes: removing shells and red skins of peanuts, squeezing at low temperature to obtain low-temperature peanut meal, grinding, degreasing, grinding and sieving the low-temperature peanut meal, adding deionized water, adjusting the pH to 8.5-9.5 by using a NaOH solution, stirring in a water bath for 1.5-2.5 hours, centrifuging, collecting supernatant, adding an HCl solution into the supernatant, adjusting the pH to 4-5, centrifuging for 10min, removing the supernatant, collecting precipitates, and carrying out vacuum freeze drying on the precipitates to obtain peanut protein.
In one embodiment, the peanut protein is prepared by the steps of: peanut (red skin removing) → low-temperature pressing (45-55 ℃) → peanut meal → grinding (30-60 mesh) → degreasing (n-hexane is adopted, w/v ═ 0.5-2: 10) → grinding and sieving (60-80 mesh) → deionized water (w/v ═ 0.5-2: 10) → NaOH solution → adjusting pH to 8.5-9.5 → stirring in a water bath (45-55 ℃) for 1.5-2.5 h → 3000-5000 Xg centrifuging for 5-15 min → collecting supernatant → HCl solution → adjusting pH to 4-5 → 4000 Xg centrifuging for 5-15 min → discarding supernatant → collecting precipitate → vacuum freeze drying → peanut protein. The peanut protein obtained by the method is in a solid form, namely peanut protein powder. The method for preparing the peanut protein from the peanut meal can recover the protein in the peanut meal to the maximum extent after condition optimization.
Further, the protein content of the peanut protein is 85-90%, such as 85%, 86%. 89%, 90%, etc., in a specific case the protein content of the peanut protein is 87.99%.
Further, the specific steps of electrostatic spinning in the step B include: and adding the spinning solution into an injector of electrostatic spinning equipment, installing the injector on a micro injection pump, adjusting the propelling speed of the micro injection pump, the rotating speed of a roller receiver and the spraying distance, and starting a high-voltage direct-current power supply to obtain the peanut protein/polyurethane nanofiber membrane.
Further, the receiving material of the electrostatic spinning in the step B is an aluminum foil, and the spraying distance is the shortest straight-line distance between the top end of the syringe needle and the edge of the roller receiver; the electrostatic spinning process parameters comprise: voltage: 14-22 kV; distance of syringe nozzle to roller receiver: 16-25 cm; spinning speed: 0.5-1.5 mL/h; spinning temperature is 25-35 ℃; relative humidity: 35% -55%%. It will be appreciated that the electrospinning steps described above are conventional in the art, but involve process parameters that are optimized and found to be particularly suitable for electrospinning peanut protein and polyurethane solutions.
Furthermore, the peanut protein-polyurethane nanofiber membrane is a three-dimensional network structure, the fiber diameter of the peanut protein-polyurethane nanofiber membrane is 100-900nm, and the thickness of the peanut protein-polyurethane nanofiber membrane is 0.1-2500 mu m. The size of the nanofiber membrane is suitable for preparing medical dressings.
Further, the above preparation method can be applied to laboratory operations, pilot plant operations and industrial production, and the steps such as stirring, pulverization, centrifugation and the like can be adaptively adjusted according to the production scale, for example, in laboratory operations, magnetic stirring, pulverization by mortar grinding and the like are used.
The second aspect of the invention provides an application of peanut protein in preparing a nanofiber membrane, wherein the nanofiber membrane is a peanut protein-polyurethane nanofiber membrane, the characteristics of the peanut protein and the preparation method of the peanut protein are as described above, and the peanut protein-polyurethane nanofiber membrane is prepared by any one of the methods.
The third aspect of the invention provides an application of the peanut protein-polyurethane nanofiber membrane in preparation of a medical dressing, wherein the peanut protein-polyurethane nanofiber membrane is prepared by any one of the methods.
Compared with the prior art, the invention adopts the technical scheme, and has the following technical effects:
according to the invention, natural biological macromolecular material peanut protein and polyurethane are prepared into the nanofiber membrane for the first time by an electrostatic spinning method, so that the nanofiber membrane has high porosity and simultaneously shows good mechanical and biological properties; the peanut protein is protein in peanut meal left after cold oil pressing of peanuts, and the vegetable protein nanofiber membrane is developed by taking the defatted peanut powder as a raw material, so that peanut protein resources such as the peanut meal and the like are efficiently utilized, the comprehensive utilization efficiency of the peanuts is improved, the peanuts are promoted to be further converted into high value-added products, and good economic and environmental benefits are achieved.
Drawings
Fig. 1 is a scanning electron microscope image of the polyurethane/peanut protein nanofiber membrane prepared in one embodiment of the present invention.
FIG. 2 is a diameter chart of the polyurethane/peanut protein nanofiber membrane prepared in one embodiment of the present invention.
Fig. 3 is a diagram of a polyurethane/peanut protein nanofiber membrane prepared in an embodiment of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby. The experimental methods of the following examples, which are not specified under specific conditions, are generally determined according to national standards. If there is no corresponding national standard, it is carried out according to the usual international standards, to the conventional conditions or to the conditions recommended by the manufacturer. Unless otherwise indicated, all parts are parts by weight and all percentages are percentages by mass. Unless defined or indicated otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition, any methods and materials similar or equivalent to those described herein can be used in the methods of the present invention.
In the following examples, peanut proteins were prepared as follows:
peanuts (hulling and red skin removal) → low-temperature pressing (50 ℃) → peanut meal → grinding (40-mesh sieving) → defatting (with n-hexane, w/v ═ 1: 10) → grinding and sieving (40-mesh) → deionized water (w/v ═ 1: 10) → 1mol/L NaOH solution → adjusting pH to 9.0 → water-bath stirring (50 ℃) for 2h → 4000 xg centrifugation for 10min → collecting supernatant → 1mol/L HCl solution → adjusting pH to 4.5 → 4000 xg centrifugation for 10min → discarding supernatant → collecting precipitate → vacuum freeze drying → peanut protein;
the preparation method of the peanut protein is technically improved according to the method in the existing literature, so that the prepared peanut protein can recover protein in peanut meal to the maximum extent and is easier to carry out electrostatic spinning. The prepared peanut protein is solid powder, has the protein content of 87.99 percent and the molecular weight of 14-66kDa, can be used for preparing a polyurethane-peanut protein nanofiber membrane in the following embodiment, and the polyurethane adopted in the following embodiment is PU (1180A10) and is extrusion grade.
Example 1
The embodiment is a preparation method of a peanut protein-polyurethane nanofiber membrane and an optimization process of each parameter of the peanut protein-polyurethane nanofiber membrane, and the preparation method comprises the following steps: dissolving polyurethane in a solvent, stirring and dissolving to obtain a polyurethane solution; adding peanut protein and polyurethane into peanut protein according to a certain mass ratio, uniformly stirring to obtain a polyurethane-peanut protein spinning solution, then carrying out electrostatic spinning, and receiving the nanofilaments by using an aluminum foil to obtain the peanut protein-polyurethane nanofiber membrane.
In the above operation process, the process conditions such as the selection of the solvent, the dissolution step of the raw materials, the raw material ratio, the stirring conditions, the electrostatic spinning parameters and the like are optimized, and the specific steps are as follows:
1. optimization of solvent and its amount
Hexafluoroisopropanol is used as a solvent, 0.5% -8% (0.5% is used as a step length for carrying out experiments, 0.5%, 1%, 1.5%. 7.5%, 8%) of polyurethane solution is prepared for carrying out electrostatic spinning experiments, the spinning voltage is constant, the spinning speed is constant, the receiving distance is constant, and the polyurethane nanofiber membrane is obtained. Observing the appearance of the fiber, comparing the fiber diameter, and obtaining the optimal polyurethane concentration of 1-5% suitable for spinning according to the experimental result.
2. Optimization of solution formulation procedure
Under the condition that other operation parameters are not changed, a two-step method (respectively preparing a polyurethane solution and a peanut protein solution and then stirring at room temperature to fully mix the polyurethane solution and the peanut protein solution) and a blending method (firstly preparing a polyurethane solution with a certain concentration, then fully stirring the peanut protein (peanut protein: polyurethane) in a certain mass ratio to obtain a spinning solution) are respectively adopted for electrospinning. The appearance of the fiber is observed, and according to the experimental result, the dosage of the solvent can be effectively reduced by adopting a blending method, and the method is more suitable for spinning.
3. Optimization of raw material ratio
Peanut protein/polyurethane are selected to be respectively subjected to experiments according to the mass ratio of 1:1, 1:2, 1:3, 1:4, 1:5, 2:1, 2:3, 3:1, 3:2, 4:1, 4:3 and 5:1, the spinning voltage is constant, the spinning speed is constant, the receiving distance is constant, peanut protein nanofibers are prepared, the fiber morphology is observed, and the fiber diameters are compared. According to the experimental result, the mass ratio of the obtained polyurethane to the peanut protein is as follows: 1:5-5:1.
4. Optimization of agitation conditions
The prepared solution is placed in water bath at 30 ℃, 40 ℃, 45 ℃ and 60 ℃ under room temperature and is magnetically stirred, and the dissolution effect of the solution is observed. According to the experimental result, the dissolution can be realized at the temperature, but the stirring time and the cost are both suitable when the magnetic stirring dissolution is carried out at 40 ℃.
5. Optimization of electrospinning parameters
The spinning voltage is respectively 10 KV, 12 KV, 14KV, 16KV, 18KV, 20KV and 24KV, the spinning speed is constant, the peanut protein concentration is constant, the receiving distance is constant, the peanut protein nanofiber is prepared, the fiber morphology is observed, and the fiber diameter is compared. According to the experimental result, the suitable spinning voltage is 14-22 kV.
Selecting spinning speeds of 0.2, 0.5, 0.8 and 1, 1.5 and 2.0mL/h respectively to perform single-factor experiments, ensuring that the spinning voltage is constant, the peanut protein concentration is constant, the receiving distance is constant, preparing the peanut protein nanofiber, observing the fiber morphology, and comparing the fiber diameters. According to the experimental result, the proper spinning speed is 0.5-1.5 mL/h.
Example 2
This embodiment is a preferred method for preparing a peanut protein-polyurethane nanofiber membrane, which comprises:
dissolving 0.375g of polyurethane in 15ml of hexafluoroisopropanol, and performing magnetic stirring in a water bath at 40 ℃ until the polyurethane is completely dissolved to obtain a polyurethane solution with the mass fraction of 2.5% (m/v); then, according to the mass ratio of peanut protein to polyurethane of 3:1, dissolving 1.125g of peanut protein solid in the solution, and magnetically stirring the mixture in a water bath at 40 ℃ until the mixture is uniformly mixed; obtaining polyurethane and peanut protein solution with the mass fraction of 10%. And (3) carrying out electrospinning on the obtained peanut protein/polyurethane solution, wherein the electrospinning parameter is 18kV, the receiving distance is 20cm, the spinning speed is 0.8ml/h, the spinning temperature is 30 ℃, and the spinning humidity is 45%. And (3) receiving the nano-filaments generated by spraying by using the aluminum foil as a receiver to obtain the peanut protein/polyurethane nanofiber membrane.
The scanning electron microscope image of the polyurethane/peanut protein nanofiber membrane prepared in this example is shown in fig. 1, the diameter image of the nanofiber membrane is shown in fig. 2, and the actual nanofiber membrane is shown in fig. 3. From the above results, the fiber diameter of the polyurethane/peanut protein nanofiber membrane is 100-900nm, and the thickness is 0.1-2500 μm.
Example 3
This embodiment is a preferred method for preparing a peanut protein-polyurethane nanofiber membrane, which comprises:
dissolving 0.5g of polyurethane in 15ml of hexafluoroisopropanol, and performing magnetic stirring in a water bath at 40 ℃ until the polyurethane is completely dissolved to obtain a polyurethane solution with the mass fraction of 3% (m/v); then, according to the mass ratio of peanut protein to polyurethane of 2:1, dissolving 1g of peanut protein solid in the solution, and magnetically stirring at room temperature until the peanut protein solid and the solution are uniformly mixed; obtaining polyurethane and peanut protein solution with the mass fraction of 10%. And (3) carrying out electrospinning on the obtained peanut protein/polyurethane spinning solution, wherein the electrospinning parameter is voltage 16kV, the receiving distance is 18cm, the spinning speed is 0.5ml/h, the spinning temperature is 25 ℃, and the spinning humidity is 50%. And (3) receiving the nano-filaments generated by spraying by using the aluminum foil as a receiver to obtain the peanut protein/polyurethane nanofiber membrane. The scanning electron microscope image and the diameter image of the nanofiber membrane of the polyurethane/peanut protein nanofiber membrane prepared in this example are slightly different from the related characteristics of the nanofiber membrane of the polyurethane/peanut protein nanofiber membrane prepared in example 1, but the overall performance is not much different.
Example 4
This embodiment is a preferred method for preparing a peanut protein-polyurethane nanofiber membrane, which comprises:
dissolving 0.75g of polyurethane in 15ml of hexafluoroisopropanol, and performing magnetic stirring in a water bath at 40 ℃ until the polyurethane is completely dissolved to obtain a polyurethane solution with the mass fraction of 5% (m/v); then, according to the mass ratio of the peanut protein to the polyurethane of 1:1, dissolving 0.75g of peanut protein solid in the solution, and magnetically stirring at room temperature until the peanut protein solid is uniformly mixed; obtaining polyurethane and peanut protein solution with the mass fraction of 10%. And (3) carrying out electrospinning on the obtained peanut protein/polyurethane spinning solution, wherein electrostatic spinning parameters are voltage of 14kV, receiving distance is 20cm, spinning speed is 0.8ml/h, spinning temperature is 30 ℃, and spinning humidity is 45%. And (3) receiving the nano-filaments generated by spraying by using the aluminum foil as a receiver to obtain the peanut protein/polyurethane nanofiber membrane. The scanning electron microscope image and the diameter image of the nanofiber membrane of the polyurethane/peanut protein nanofiber membrane prepared in this example are slightly different from the related characteristics of the nanofiber membrane of the polyurethane/peanut protein nanofiber membrane prepared in example 1, but the overall performance is not much different.
Example 5
This embodiment is a preferred method for preparing a peanut protein-polyurethane nanofiber membrane, which comprises:
dissolving 0.3g of polyurethane in 15ml of hexafluoroisopropanol, and performing magnetic stirring in a water bath at 40 ℃ until the polyurethane is completely dissolved to obtain a polyurethane solution with the mass fraction of 2% (m/v); then, according to the mass ratio of peanut protein to polyurethane of 4:1, dissolving 1.2g of peanut protein solid in the solution, and magnetically stirring at room temperature until the peanut protein solid is uniformly mixed; obtaining polyurethane and peanut protein solution with the mass fraction of 10%. And (3) carrying out electrostatic spinning on the obtained peanut protein/polyurethane solution, wherein the electrostatic spinning parameter is voltage 20kV, the receiving distance is 20cm, the spinning speed is 1.0ml/h, the spinning temperature is 30 ℃, and the spinning humidity is 50%. And (3) receiving the nano-filaments generated by spraying by using the aluminum foil as a receiver to obtain the peanut protein/polyurethane nanofiber membrane. The scanning electron microscope image and the diameter image of the nanofiber membrane of the polyurethane/peanut protein nanofiber membrane prepared in this example are slightly different from the related characteristics of the nanofiber membrane of the polyurethane/peanut protein nanofiber membrane prepared in example 1, but the overall performance is not much different.
Example 6
This embodiment is a preferred method for preparing a peanut protein-polyurethane nanofiber membrane, which comprises:
dissolving 0.25g of polyurethane in 15ml of hexafluoroisopropanol, and performing magnetic stirring in a water bath at 40 ℃ until the polyurethane is completely dissolved to obtain a polyurethane solution with the mass fraction of 1.6% (m/v); then, according to the mass ratio of the peanut protein to the polyurethane of 5:1, dissolving 1.25g of peanut protein solid in the solution, and magnetically stirring at room temperature until the peanut protein solid is uniformly mixed; obtaining polyurethane and peanut protein solution with the mass fraction of 10%. And (3) carrying out electrostatic spinning on the obtained peanut protein/polyurethane solution, wherein electrostatic spinning parameters are voltage 22kV, receiving distance is 24cm, spinning speed is 1.5ml/h, spinning temperature is 25 ℃, and spinning humidity is 55%. And (3) receiving the nano-filaments generated by spraying by using the aluminum foil as a receiver to obtain the peanut protein/polyurethane nanofiber membrane. The scanning electron microscope image and the diameter image of the nanofiber membrane of the polyurethane/peanut protein nanofiber membrane prepared in this example are slightly different from the related characteristics of the nanofiber membrane of the polyurethane/peanut protein nanofiber membrane prepared in example 1, but the overall performance is not much different.
And (3) performance measurement:
the analysis and measurement were performed on the peanut protein-polyurethane nanofiber membrane prepared by the preparation method described in example 1, the nanofiber membrane prepared only from peanut protein, and the nanofiber membrane prepared only from polyurethane, wherein the nanofiber membrane prepared from peanut protein has poor characteristics, is fragile and breakable in a dry state, and thus no corresponding test data was obtained. The results of the above measurements are shown in the following table:
the comparison of the table shows that compared with the single polyurethane nanofiber membrane, the peanut protein-polyurethane nanofiber membrane prepared by the invention has certain differences in porosity, hydrophilicity/hydrophobicity and mechanical properties, but still keeps good toughness and mechanical strength, keeps good bioactivity of peanut protein, and is suitable for preparing medical dressings.
According to the embodiment, the natural biological macromolecular material peanut protein and polyurethane are firstly prepared into the nanofiber membrane through electrostatic spinning, so that the nanofiber membrane has high porosity and simultaneously shows good mechanical and biological properties; and the plant protein nanofiber membrane is developed by taking the defatted peanut powder as a raw material, so that peanut protein resources such as peanut meal and the like are favorably and efficiently utilized, the peanuts are promoted to be further converted into products with high added values, and the plant protein nanofiber membrane has good economic and environmental benefits.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (3)
1. A preparation method of a peanut protein-polyurethane nanofiber membrane is characterized by comprising the following steps:
step A: dissolving polyurethane in a solvent, and stirring until the polyurethane is completely dissolved to obtain a polyurethane solution; dissolving peanut protein in the polyurethane solution, and stirring until the peanut protein and the polyurethane solution are uniformly mixed to obtain a polyurethane-peanut protein solution;
and B: b, performing electrostatic spinning by using the polyurethane-peanut protein solution in the step A to prepare a peanut protein-polyurethane nanofiber membrane;
in the step A, the mass fraction of the polyurethane solution is 1% -5%, and the mass ratio of the polyurethane to the peanut protein is 1:5-5: 1; the solvent is hexafluoroisopropanol; the molecular weight of the peanut protein is 14-66 kDa; the average molecular weight of the polyurethane is 8-12 ten thousand;
before step A, the method also comprises the step a: preparing peanut protein; wherein the step a comprises: removing shells and red skins of peanuts, squeezing at a low temperature to obtain low-temperature peanut meal, grinding, degreasing, grinding and sieving the low-temperature peanut meal, adding deionized water, adjusting the pH to 8.5-9.5 by using a NaOH solution, stirring in a water bath for 1.5-2.5 hours, centrifuging, collecting supernatant, adding an HCl solution into the supernatant, adjusting the pH to 4-5, centrifuging for 10min, removing the supernatant, collecting precipitate, and performing vacuum freeze drying on the precipitate to obtain peanut protein;
in the step A, the stirring temperature of the polyurethane dissolved in the solvent is 40 ℃; the stirring temperature of the peanut protein dissolved in the polyurethane solution is room temperature or 40 ℃;
b, the receiving material of the electrostatic spinning in the step B is an aluminum foil, and the spraying distance is the shortest straight-line distance between the top end of the syringe needle and the edge of the roller receiver; wherein the electrostatic spinning process parameters comprise: voltage: 14-22 kV; distance of syringe nozzle to roller receiver: 16-25 cm; spinning speed: 0.5-1.5 mL/h; spinning temperature is 25-35 ℃; relative humidity: 35-55%; and
the fiber diameter of the peanut protein-polyurethane nanofiber membrane prepared in the step B is 100-900nm, and the thickness of the peanut protein-polyurethane nanofiber membrane is 0.1-2500 mu m.
2. The application of the peanut protein in preparing the nanofiber membrane is characterized in that the nanofiber membrane is a peanut protein-polyurethane nanofiber membrane; the peanut protein-polyurethane nanofiber membrane is prepared by the preparation method of claim 1.
3. The application of the peanut protein-polyurethane nanofiber membrane in preparing medical dressings is characterized in that the peanut protein-polyurethane nanofiber membrane is prepared by the preparation method according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010672723.1A CN111778635B (en) | 2020-07-14 | 2020-07-14 | Preparation method of peanut protein-polyurethane nanofiber membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010672723.1A CN111778635B (en) | 2020-07-14 | 2020-07-14 | Preparation method of peanut protein-polyurethane nanofiber membrane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111778635A CN111778635A (en) | 2020-10-16 |
| CN111778635B true CN111778635B (en) | 2021-11-02 |
Family
ID=72767614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010672723.1A Active CN111778635B (en) | 2020-07-14 | 2020-07-14 | Preparation method of peanut protein-polyurethane nanofiber membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111778635B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112870441A (en) * | 2021-02-09 | 2021-06-01 | 河南工业大学 | Peanut protein isolate-polylactic acid nanofiber membrane and preparation method and application thereof |
| CN114045574A (en) * | 2021-11-15 | 2022-02-15 | 华峰化学股份有限公司 | Preparation method of skin-friendly polyurethane elastic fiber |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584152A (en) * | 2004-05-25 | 2005-02-23 | 宋代轮 | Preparing method for peanut protein modified povel fibre |
| CN1683618A (en) * | 2005-03-14 | 2005-10-19 | 东华大学 | Plant protein/polyurethane/polyacrylonitrile blended composite fiber and preparing process |
| CN104018247A (en) * | 2014-06-13 | 2014-09-03 | 东华大学 | Preparation method of tubular polyurethane/keratin composite nanofiber material |
| JP2016519222A (en) * | 2013-03-15 | 2016-06-30 | アーセナル メディカル， インコーポレイテッド | Core-sheath fiber and method for making it and method for using it |
| CN109082728A (en) * | 2017-06-13 | 2018-12-25 | 山东圣泉新材料股份有限公司 | A kind of vegetable protein nanofiber and preparation method thereof containing graphene |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168858C (en) * | 2002-01-04 | 2004-09-29 | 李官奇 | Plant protein synthetic fiber and its manufacturing method |
| CN102586942B (en) * | 2012-03-18 | 2013-09-25 | 山东省花生研究所 | Method for preparing peanut protein composite fiber by using ionic liquid |
| CN106676669A (en) * | 2016-11-18 | 2017-05-17 | 陕西聚洁瀚化工有限公司 | Preparation method of wheat protein/polyvinyl alcohol composite nano fibers |
| CN108691028B (en) * | 2018-05-28 | 2019-07-23 | 武汉大学 | A kind of preparation method of polylactic acid/soybean protein isolate composite nano fiber and obtained nanofiber and application |
-
2020
- 2020-07-14 CN CN202010672723.1A patent/CN111778635B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584152A (en) * | 2004-05-25 | 2005-02-23 | 宋代轮 | Preparing method for peanut protein modified povel fibre |
| CN1683618A (en) * | 2005-03-14 | 2005-10-19 | 东华大学 | Plant protein/polyurethane/polyacrylonitrile blended composite fiber and preparing process |
| JP2016519222A (en) * | 2013-03-15 | 2016-06-30 | アーセナル メディカル， インコーポレイテッド | Core-sheath fiber and method for making it and method for using it |
| CN104018247A (en) * | 2014-06-13 | 2014-09-03 | 东华大学 | Preparation method of tubular polyurethane/keratin composite nanofiber material |
| CN109082728A (en) * | 2017-06-13 | 2018-12-25 | 山东圣泉新材料股份有限公司 | A kind of vegetable protein nanofiber and preparation method thereof containing graphene |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111778635A (en) | 2020-10-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111778635B (en) | Preparation method of peanut protein-polyurethane nanofiber membrane | |
| Salas et al. | Synthesis of soy protein–lignin nanofibers by solution electrospinning | |
| CN1247837C (en) | Method for producing fiber and film of silk and silk-like material | |
| Wang et al. | Preparation of electrospun chitosan/poly (ethylene oxide) composite nanofibers reinforced with cellulose nanocrystals: Structure, morphology, and mechanical behavior | |
| CN103993380B (en) | A kind of preparation method of Chitosan Fiber With High Tenacity | |
| Kim et al. | Effect of degumming ratio on wet spinning and post drawing performance of regenerated silk | |
| CN100368610C (en) | Blending nanometer fibre with silk protein and vinol and production thereof | |
| CN103320886A (en) | Bionic regenerated silk fibroin filament fiber and preparation method thereof | |
| CN103469351A (en) | Preparation method of vitamin C-loaded silk fibroin/hyaluronic acid composite nanofibers | |
| CN104818543A (en) | Modified polylactic acid fiber excellent in performance | |
| Faragò et al. | A dry powder formulation from silk fibroin microspheres as a topical auto-gelling device | |
| CN111910282B (en) | Waste feather regenerated pure keratin fiber and preparation method thereof | |
| CN106367839B (en) | A kind of preparation method producing flame retardant polyester fiber using returnable bottle piece | |
| Mehetre et al. | Isolation and characterization of bionanofibers from Moringa oleifera gum as a platform for drug delivery | |
| CN112853535A (en) | Composite fiber material for wig and preparation method thereof | |
| CN101619501B (en) | Preparation method of high molecular superfine fiber with controllable diameter | |
| Ma et al. | Preparation of chitosan fibers using aqueous ionic liquid as the solvent | |
| CN111349977A (en) | Wheat gluten protein nano-fiber and preparation method and application thereof | |
| CN105088398A (en) | Feather composite fibers added with negative ion polyester short fibers | |
| CN114163684A (en) | Method for directly extracting fibroin nanofibers from waste silkworm cocoons and recovering hydrolyzed silk protein and extracting solution | |
| CN1667166A (en) | Acrylic fiber-crosslinking plant protein composite fiber and method for manufacturing the same | |
| CN101377023B (en) | Method for producing nano-scale fiber by wheat mucedin | |
| CN105200558A (en) | Preparation method of sericulture fiber material | |
| CN111041603A (en) | Preparation method of fibroin/microorganism-based polymer solution and preparation method of composite nanofiber of fibroin/microorganism-based polymer solution | |
| CN105088401A (en) | Chinese herbal medicine anti-bacterial health care composite down fiber textile material |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |