CN101372678A - Keratin degrading bacteria NJY1 - Google Patents
Keratin degrading bacteria NJY1 Download PDFInfo
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- CN101372678A CN101372678A CNA2008101559582A CN200810155958A CN101372678A CN 101372678 A CN101372678 A CN 101372678A CN A2008101559582 A CNA2008101559582 A CN A2008101559582A CN 200810155958 A CN200810155958 A CN 200810155958A CN 101372678 A CN101372678 A CN 101372678A
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Abstract
The invention relates to a keratin degradating bacterium NJY1 which belongs to the high technical field of biology. The strain NJY1 is named in classification as Bacillus thuringiensis, and the culture collection number is CGMCC NO.2494. The strain is inoculated in a seed culture medium and cultured at the temperature of 37 DEG C and at the rate of 120r/min for 24h to obtain a liquid culture of the NJY1. The strain produces transparent hydrolysis rings on a feather meal culture medium, the feather ferments and is degraded in a feather fermentation culture medium, and the degradation rate of the keratin in a keratin fermentation culture medium is 72.5%. The keratin degradation bacterium degrades the keratin by the method of microbial fermentation, which is suitable for producing high-quality protein feed. The keratin degradation bacterium can be used for developing and utilizing waste feather resources of slaughtered livestock and poultry, particularly transforming the feather protein into feed, so that waste can be changed into valuables, environment is protected, sustainable development can be promoted, and the application prospect is wide.
Description
Technical field
A kind of keratin degrading bacteria NJY 1 of the present invention belongs to biological high-tech field, is the method degraded Keratin sulfate that utilizes microbial fermentation, is applicable to the production of high-quality protein feed.
Background technology
In recent years, also more and more higher to the demand of feed protein along with developing rapidly of China's livestock industry, at present, China mainly solves the deficiency of protein resource by imported fish meal, is the focus of studying at present so how to develop new protein resource.China's feather resource is very abundant, and annual production reaches the hundreds of thousands of ton, the discarded huge waste that not only causes resource of a large amount of feathers, and contaminate environment.The protein content height of poultry feather, generally 75～90%, and the amino acid kind is more complete, except that Methionin and methionine content are low slightly, the composition of other animal indispensable amino acid is higher than fish meal, particularly cystine be various native protein feeds, the deficiency of having replenished egg amino acid to a certain extent.But poultry feather mainly is made of Keratin sulfate, hydrophobic amino acid is distributed in keratic periphery, a small amount of hydrophilic amino acid is contained within the inside of peptide chain and protein skeleton, form many disulfide linkage between the peptide chain, be cord structures, character is extremely stable, and utmost point indissoluble is separated in water, hydrochloric acid and rare salts solution.As unprocessed, the common enzyme (trypsinase, stomach en-, papoid etc.) in the digestive tube is difficult to its decomposition, and livestock and poultry are lower to its digestibility.Therefore set up that suitable feasible method changes feather protein into digestible protein and environment not only can be turned waste into wealth, be protected to amino acid, can also promote sustainable development, and have broad application prospects.Handle feather with microbial fermentation, the cleavable disulfide linkage, protein denaturation and amino acid whose loss have been reduced, improve amino acid whose digestibility in the feather meal, increased and to digest amino acid, improved the balance of indispensable amino acid, and reduced nitrogen eliminating amount, reduced polluted by nitrogen, the nutritive value of feather meal significantly improves, and the economic return that is obtained is obviously than high many of the feather meal of ordinary method processing.
Summary of the invention
Technical problem
The objective of the invention is to begin to occur the problem of critical shortage, provide a kind of keratin degrading efficient bacterial strain, be used to develop new protein fodder at feed protein resource in the production practice.
Technical scheme
The invention provides a kind of keratin degrading bacteria, this bacterial strain NJY1 called after of classifying: bacillus thuringiensis (Bacillus thuringiensis), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 05 12nd, 2008, culture presevation number is CGMCC NO.2494.Main biological characteristics is G
+, thalline is shaft-like, about 0.6～0.7 μ m of size * 1.4～1.5 μ m has flagellum; Catalase, glucose, sucrose produce acid, utilize Citrate trianion, hydrolyzed casein, gelatin, the starch positive; Pectinose, it is negative that N.F,USP MANNITOL, wood sugar produce acid.The Genbank number of landing of this bacterial strain 16S rDNA is EU624205.
The enlarged culturing method of above-mentioned bacterial strains NJY1 is:
1) prescription of seed culture medium is, contains in the 1L distilled water: 6.0g peptone, 4.0g beef extract, 2.0g yeast decoction, 5.0g sodium-chlor.Heating for dissolving is regulated pH7.5,121 ℃ of autoclaving 15min.
2) the above-mentioned bacterial strain NJY1 that obtains is inoculated in the Erlenmeyer flask of the 250mL that the 50mL seed culture medium is housed, under 37 ℃ of conditions of temperature, the rotating speed with 120r/min on shaking table is cultivated 24h, obtains NJY1 bacterium liquid, and thalline quantity is 4 * 10
11CFU/mL.
Keratin degrading bacteria NJY 1 can be grown on the feather meal substratum, produces transparent hydrolysis circle.Described feather meal culture medium prescription is: contain in the 1L distilled water: 5.0g sodium-chlor, 20.0g feather meal, 20.0g agar powder.Heating for dissolving is regulated pH7.5,121 ℃ of autoclaving 15min.
With keratin degrading bacteria NJY 1 fermentation Keratin sulfate fermention medium, the keratin degrading rate reaches 72.5%.The prescription of Keratin sulfate fermention medium is to contain in the 1L distilled water: 0.01g calcium chloride, and 0.1g sal epsom, 0.5g sodium-chlor, the 0.3g dipotassium hydrogen phosphate, the 0.4g potassium primary phosphate, the 20g feather meal is regulated pH7.5,121 ℃ of autoclaving 15min.
Beneficial effect
The invention provides keratin degrading bacteria NJY 1.Keratin degrading bacteria NJY 1 can be grown on the feather meal substratum, produces transparent hydrolysis circle.With keratin degrading bacteria NJY 1 fermentation Keratin sulfate fermention medium, the keratin degrading rate reaches 72.5%.The present invention also provides the enlarged culturing method of keratin degrading bacteria NJY 1, and the thalline quantity in the product reaches 4 * 10
11CFU/mL.
Keratin degrading bacteria NJY 1 can be used for the development and use that livestock and poultry are butchered the waste feathers resource; the huge livestock and poultry of output are butchered feather and can be used as the high-quality protein feed source after with efficient strain fermentation; particularly feather protein is changed into feed; not only can turn waste into wealth, protect environment; can also promote sustainable development, have broad application prospects.
Embodiment
Embodiment 1: efficient strains separation
Collected specimens 2g from the particular surroundingss such as soil of piling up feather adds the 10mL stroke-physiological saline solution, and vibration 1min leaves standstill 5min, draws supernatant liquor inoculation nutrient broth medium, cultivates 48h under 37 ℃ of conditions of temperature.
With above-mentioned cultivation bacterial classification streak inoculation feather meal culture medium flat plate, cultivate 24h under 37 ℃ of conditions of temperature.Described feather meal culture medium prescription is to contain in the 1L distilled water: 5.0g sodium-chlor, 20.0g feather meal, 20.0g agar powder.Heating for dissolving is regulated pH7.5,121 ℃ of autoclaving 15min.
Carry out pure culture to producing the bigger bacterium of hydrolysis circle in the above-mentioned substratum.Obtain the bigger strain bacterium of hydrolysis of keratin ability, called after: NJY1.This bacterial strain NJY1 called after of classifying: bacillus thuringiensis (Bacillus thuringiensis), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 05 12nd, 2008, culture presevation number is CGMCC NO.2494.Main biological characteristics is G+, and thalline is shaft-like, and about 0.6～0.7 μ m of size * 1.4～1.5 μ m has flagellum; Catalase, glucose, sucrose produce acid, utilize Citrate trianion, hydrolyzed casein, gelatin, the starch positive; Pectinose, it is negative that N.F,USP MANNITOL, wood sugar produce acid.The Genbank number of landing of this bacterial strain 16S rDNA is EU624205.
Embodiment 2: the enlarged culturing of above-mentioned bacterial strains NJY1
The prescription of seed culture medium is to contain in the 1L distilled water: 6.0g peptone, 4.0g beef extract, 2.0g yeast decoction, 5.0g sodium-chlor.Heating for dissolving is regulated pH7.5,121 ℃ of autoclaving 15min.
The above-mentioned bacterial strain NJY1 that obtains is inoculated in the Erlenmeyer flask of the 250mL that the 50mL seed culture medium is housed, and under 37 ℃ of conditions of temperature, the rotating speed with 120r/min on shaking table is cultivated 24h, obtains the NJY1 liquid culture, and thalline quantity is 4 * 10
11CFU/mL.
Embodiment 3: utilize bacterial strain NJY1 fermentative degradation feather in the feather fermention medium
Get the NJY1 liquid culture 1mL that embodiment 2 obtains, be inoculated in the 500mL Erlenmeyer flask that 100mL feather fermention medium is housed, under 37 ℃ of conditions of temperature, the rotating speed with 180r/min on shaking table was cultivated 3 days.The prescription of feather fermention medium is to contain in the 1L distilled water: 0.01g calcium chloride, and 0.1g sal epsom, 0.5g sodium-chlor, the 0.3g dipotassium hydrogen phosphate, the 0.4g potassium primary phosphate, 160 complete feathers are regulated pH8.0,121 ℃ of autoclaving 15min.Feather in substratum increase in time feather sprig before this comes off gradually, and feather stem ruptures gradually then, and feather is degraded fully after 3 days, and substratum becomes the suspension of homogeneous.
Embodiment 4: utilize bacterial strain NJY1 fermentative degradation Keratin sulfate in the Keratin sulfate fermention medium
Get the NJY1 liquid culture 1mL that embodiment 2 obtains, be inoculated in the 500mL Erlenmeyer flask that 100mL Keratin sulfate fermention medium is housed, under 37 ℃ of conditions of temperature, the rotating speed with 180r/min on shaking table was cultivated 3 days.The prescription of Keratin sulfate fermention medium is to contain in the 1L distilled water: 0.01g calcium chloride, and 0.1g sal epsom, 0.5g sodium-chlor, the 0.3g dipotassium hydrogen phosphate, the 0.4g potassium primary phosphate, the 20g feather meal is regulated pH7.5,121 ℃ of autoclaving 15min.Measure the keratin degrading rate according to the variation of residue weight, cultivate that the keratin degrading rate is 72.5% after three days.
Claims (3)
1. keratin degrading bacteria NJY 1, it is characterized in that, this bacterial strain NJY1 called after of classifying: bacillus thuringiensis (Bacillus thuringiensis), be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on 05 12nd, 2008, culture presevation number is CGMCC NO.2494.
2. keratin degrading bacteria as claimed in claim 1, its enlarged culturing method is:
(1) preparation of seed culture medium: contain 6.0g peptone, 4.0g beef extract, 2.0g yeast decoction and 5.0g sodium-chlor in the 1L distilled water, heating for dissolving is regulated pH7.5,121 ℃ of autoclaving 15min;
(2) the described bacterial strain NJY1 of claim 1 is inoculated in the Erlenmeyer flask of the 250mL that the 50mL seed culture medium is housed, under 37 ℃ of conditions of temperature, the rotating speed with 120r/min on shaking table is cultivated 24h, obtains NJY1 bacterium liquid.
3. claim 1 or 2 described keratin degrading bacterias contain keratin substances such as feather and animal hair to obtain the application aspect small molecular protein, polypeptide, the amino acid in degraded.
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| CN2008101559582A CN101372678B (en) | 2008-10-22 | 2008-10-22 | Keratin degrading bacteria NJY1 |
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| CN2008101559582A CN101372678B (en) | 2008-10-22 | 2008-10-22 | Keratin degrading bacteria NJY1 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102326668A (en) * | 2011-08-24 | 2012-01-25 | 农业部沼气科学研究所 | Method for anaerobically degrading feather keratin with microbial strain 18D-TA |
| CN102696863A (en) * | 2012-07-04 | 2012-10-03 | 天津科技大学 | Method for preparing feather meal additive via mixed solid-state fermentation |
| CN103088098A (en) * | 2013-01-17 | 2013-05-08 | 安徽华强羽绒有限公司 | Method for producing peptone by taking poultry feather as raw material |
| CN104450571A (en) * | 2014-12-04 | 2015-03-25 | 湖南农业大学 | Bacillus thuringiensis strain for efficient degradation of fly larvae protein |
| CN105820979A (en) * | 2016-04-25 | 2016-08-03 | 湖北工业大学 | Feather degradation strain and application thereof |
| CN110663817A (en) * | 2018-11-29 | 2020-01-10 | 东北农业大学 | Application of lactococcus lactis KDLL2016-01 in preparation of protein feed by fermenting feathers |
| CN110669685A (en) * | 2018-11-29 | 2020-01-10 | 东北农业大学 | Probiotic capable of degrading feather keratin and application thereof |
| CN114540252A (en) * | 2022-03-31 | 2022-05-27 | 山东省农业科学院 | Microbacterium P6 for converting livestock and poultry breeding waste and application |
-
2008
- 2008-10-22 CN CN2008101559582A patent/CN101372678B/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102326668A (en) * | 2011-08-24 | 2012-01-25 | 农业部沼气科学研究所 | Method for anaerobically degrading feather keratin with microbial strain 18D-TA |
| CN102696863A (en) * | 2012-07-04 | 2012-10-03 | 天津科技大学 | Method for preparing feather meal additive via mixed solid-state fermentation |
| CN102696863B (en) * | 2012-07-04 | 2013-06-05 | 天津科技大学 | Method for preparing feather meal additive via mixed solid-state fermentation |
| CN103088098A (en) * | 2013-01-17 | 2013-05-08 | 安徽华强羽绒有限公司 | Method for producing peptone by taking poultry feather as raw material |
| CN103088098B (en) * | 2013-01-17 | 2014-12-31 | 安徽华强羽绒有限公司 | Method for producing peptone by taking poultry feather as raw material |
| CN104450571B (en) * | 2014-12-04 | 2017-08-25 | 湖南农业大学 | A kind of bacillus thuringiensis bacterial strain of efficient degradation fly-maggot protein |
| CN104450571A (en) * | 2014-12-04 | 2015-03-25 | 湖南农业大学 | Bacillus thuringiensis strain for efficient degradation of fly larvae protein |
| CN105820979A (en) * | 2016-04-25 | 2016-08-03 | 湖北工业大学 | Feather degradation strain and application thereof |
| CN105820979B (en) * | 2016-04-25 | 2019-04-19 | 湖北工业大学 | One plant of feather degradation bacteria strains and application |
| CN110663817A (en) * | 2018-11-29 | 2020-01-10 | 东北农业大学 | Application of lactococcus lactis KDLL2016-01 in preparation of protein feed by fermenting feathers |
| CN110669685A (en) * | 2018-11-29 | 2020-01-10 | 东北农业大学 | Probiotic capable of degrading feather keratin and application thereof |
| CN110663817B (en) * | 2018-11-29 | 2022-09-27 | 东北农业大学 | Application of lactococcus lactis KDLL2016-01 in preparation of protein feed by fermenting feathers |
| CN110669685B (en) * | 2018-11-29 | 2022-11-22 | 东北农业大学 | Probiotic capable of degrading feather keratin and application thereof |
| CN114540252A (en) * | 2022-03-31 | 2022-05-27 | 山东省农业科学院 | Microbacterium P6 for converting livestock and poultry breeding waste and application |
| CN114540252B (en) * | 2022-03-31 | 2022-09-13 | 山东省农业科学院 | Microbacterium P6 for converting livestock and poultry breeding waste and application |
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|---|---|
| CN101372678B (en) | 2010-06-09 |
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