CN101371857A - Composite medicament for inhibiting tumor and preparation method thereof as well as technique field - Google Patents
Composite medicament for inhibiting tumor and preparation method thereof as well as technique field Download PDFInfo
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- CN101371857A CN101371857A CNA2007100765910A CN200710076591A CN101371857A CN 101371857 A CN101371857 A CN 101371857A CN A2007100765910 A CNA2007100765910 A CN A2007100765910A CN 200710076591 A CN200710076591 A CN 200710076591A CN 101371857 A CN101371857 A CN 101371857A
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Abstract
The invention discloses a compound medicine for inhibiting tumor; the medicine consists of coenzyme Q10, polysaccharide and triterpene. The medicine has the functions of enhancing immunity, resisting the proliferation of tumor cells, inducing the apoptosis of cells and interfering with the synthesis of tubulin of tumor cells, so as to play the inhibition role. Furthermore, the invention also discloses a preparation method of the compound medicine for inhibiting tumor by processing materials through semi-synthesis method or supercritical CQ2 method.
Description
Technical field
The present invention relates to a kind of combination drug that suppresses tumor, and the preparation method of the combination drug made from semi-synthesis method, microwave low-temperature solvent extraction feed that can suppress tumor.
Background technology
Tumor is the principal disease of serious threat human health and life, and its M ﹠ M constantly rises, and enjoys people to pay close attention to.Number of research projects has all been carried out to the control of tumor in countries in the world, obtains significant progress.But, under being free from side effects, can effectively suppress the tomour specific cell, reach the domestic specific medicament that still do not have at present in the world.
Tumour immunity escape be meant tumor cell by certain mechanism escape body immune system to its supervision with kill and wound, thereby the generation, development, transfer and the rebound phenomenon that cause tumor, found that so far number of mechanisms participates in Immune escape of tumor, can be summarized as two aspects: the one, from the change of tumor cell and tumor antigen itself, as tumor cell MHC or its stimulation molecule lack as, the downward modulation that immunogenicity lowers, angtigen presentation related gene (TAP, LMP etc.) is expressed of tumor antigen etc.; The 2nd, from the variation of body immune system function, as can not discern in tumorigenic early immune system low-level tumor associated antigen by marrow sample SC (Myeloia Suppressorcells, MSC) and the T cell that causes of regulatory T cells (Treg) tolerance of tumor associated antigen and inhibition, the sole duty antigen of function are cell function disappearance etc.
The interaction of tumor cell in body immune system, promptly tumorigenic early stage, body is transferred innate immunity and acquired immune system is attacked tumor cell, and this stage immune system is occupied an leading position, and can effectively control growth of tumor and killing tumor cell.But As time goes on, tumor cell with immune system trial of strength in, tumor cell can be by to the modification of self surface antigen and change immunity identification that microenvironment around the tumor tissues escapes body and attack.In this stage, tumor causes the immune privilege sample zone that can not arrive for T cells with antigenic specificity, thereby finally causes the generation of Immune escape of tumor.Therefore, suppress tumour-specific T cell and just must solve the immune system problem earlier.
The additional coenzyme Q10 deficiency state of having corrected pathological tissues of exogenous coenzyme Q10, coenzyme Q10 plays the biological agent of resisting external intrusion with chemical substance to immune most of cell except antioxidation.
Development along with the research of tumor cell immunologic escape mechanism, many novel drugs have appearred in recent years, certain limitation treatment malignant tumor curative effect and nutritive value are arranged, and as the disclosed number of patent application of China be: (ZL03112703.7) disclosing a kind of its pharmaceutical compositions is: Agaricus blazei Murrill, Lentinus Edodes, Coriolous Dersicolor (Fr.) Quel, Ganoderma, Cordyceps mycelium and sesame spirit exosporium-broken spore; Chinese patent application number is: (93111101.3) disclose a kind of treatment liver tumor medicine: adopt a shell, the Radix Rehmanniae, Rhizoma Coptidis, Rhizoma Curcumae, Rhizoma Cyperi, Poria, Radix Paeoniae Rubra, Fructus Gardeniae, the Rhizoma Atractylodis Macrocephalae, Rhizoma Homalomenae, rhizoma sparganic, Radix Salviae Miltiorrhizae, first strain, Scolopendra etc.But these medicines can not produce direct effect because of can't switching energy and make again and keep immune system cell to tumour-specific T cell.So China's medical science does not still have specific way to suppressing tumor at present.
Summary of the invention
The object of the present invention is to provide a kind of combination drug that suppresses tumor.This medicine is widely used in and suppresses malignant tumor of liver (hepatocarcinoma, cholangiocellular carcinoma, metastatic hepatic neoplasm) to auxiliary treatment effects such as pulmonary carcinoma, rectal cancer, gastric cancer, hysteroma, mastadenoma.Also have effects such as blood fat reducing, cholesterol reducing, endocrine regulation, elimination climacteric syndrome, slow down aging, prevention alzheimer disease.
Another object of the present invention provides the preparation method of the combination drug of this inhibition tumor.
One, medicine of the present invention is made (consumption is weight percentage) by following prescription
Coenzyme Q10 (Coenzyme Q10 Capsul es) 8-20%
Polysaccharide (Ganud ermal uci dum Polysacharide) 10-35%
Triterpenes (triterpenes) 20-54%
Two, the preparation method that above-mentioned each component is made medicine of the present invention is:
(a) adopt semi-synthesis method to be processed into the coenzyme Q10 raw material;
(b) adopt the extraction of microwave low-temperature solvent to be processed into the Ganoderma spore oil raw material;
(c) with above-mentioned be after fine powder, Ganoderma spore oil mix originally, preparation system soft capsule.The combination drug soft capsule that can promptly suppress tumor.
Description of drawings
Fig. 1 is that the COQ10 group compares one with the matched group curative effect.
Fig. 2 is that the COQ10 group compares two with the matched group curative effect.
The specific embodiment
Solution of the present invention is based on medically to be familiar with and the Therapeutic Principle tumor cell immunologic escape mechanism, with reference to the modern pharmacological research achievement, presses the theory of medicine assembly, extracts plant extraction elite polymer.Make its pharmacology effectively increase the effect that generates adenosine triphosphate (ATP) in the cell and stabilizing cell membrane and enzyme body film are arranged, and suppress tissue and discharge bioactive peptide, the enhance immunity systemic effect, as strengthen macrophage phagocytic function, promote the T lymphopoiesis, strengthen the cytotoxicity of T killer cell, promote the generation of interleukin I and I and tumor necrosis factor, thereby improve the antineoplastic immune power of body, suppress indirectly or kill intravital tumor cell.In addition, also can alleviate radiotherapy and chemotherapy, improve the chemicotherapy curative effect and reduce its untoward reaction, and bring into play its more effective therapeutical effect tumor patient hemopoietic system and immune system injury.
One, coenzyme Q10 (Coenzyme Q10 Capsules)
Structural formula:
Character: the orange-yellow crystalloid powder of odorless, tasteless, oil-soluble easily decomposes in the ultraviolet light, and fusing point is: 48 ~ 500C.
Effect and purposes: coenzyme Q10 is the ingredient that has the organized enzyme of important function in the histiocyte, also is the activator of cellular metabolism and Cellular respiration.In the cell mitochondrial respiratory chain is to play hydrogen carrier in the electron transport system, has the ability of natural free radical, can strengthen the human body non-specific immunity.
In 1972, be published in the report on " chemistry---biological agent " magazine about sturcture-activity relationship, the different members that we have evaluated the CoQ group is used the activity of (gulping down) phagocyte rate in the blood flow of mouse.Qo (CoQ does not have side chain fully) does not show any change of (gulping down) phagocyte rate.CoQ4, CoQ6 and CoQ7 play some effects, and CoQ10 has the most significant effect, and this experiment has shown that the activity of different CoQS is relevant with the reinforcement of side chain lengths.
Other CoQ is certainly further confirmed by our experiment immune advantageous effect.It can reduce size and the number that is produced the mouse in-vivo tumour of tumor by chemical method, it and can increase survivor's quantity, this tumor is a kind of very effective carcinogenic injection and causing, this breakneck chemical substance is prevalent in the tar, and under a cloud be the reason that causes smoker's lung tumor, this chemical substance that causes tumor is thought to cause in the nature typical factor of tumor by scientist.
When injecting this carcinogenic the 55th day, 85% do not take CoQ that organize tumor, all got tumor by the 69th day 100%, and simultaneously have only 25% to show tumor when those have taken in the mouse of CoQ at the 55th day, 65% mouse got tumor in the time of the 69th day; 77% got tumor in the time of the 300th day.
In addition, noticeable factor is exactly the size of tumor in these two groups of mouse, and here we measure with mm2, and in the time of the 55th day, not taking CoQ group average tumor is 250mm2, and takes CoQ group average out to 95mm2; In the time of 83 days, the tumor size of not taking the CoQ group is 360mm2, has taken CoQ group average out to 170mm2; In the time of the 97th day, that group tumor size of not taking CoQ is upgraded to 930mm2, and that group of taking has only 580mm2.In having taken CoQ group mouse, have only minority to get tumor.
This experiment is to create the most desirable, the most important material of immune system vigor.When the 83rd day of experiment, not taking CoQ group mortality rate is 5%; To 111 days the time, not obeying CoQ mouse mortality rate is 50%; And that group mortality rate of having taken CoQ is 15%; In the time of the 132nd day, not taking CoQ group mortality rate is 100%; And that group mortality rate of taking is 18%; And in the time of 300 days, taking CoQ group mortality rate is 20%.In the experiment test process, can reach a conclusion thus, the effect of this life-saving of CoQ is fairly obvious.
Two, Ganoderma spore oil (Myth fungus spore oil)
Ganoderma spore oil is to adopt microwave low-temperature solvent extracting process concentration extraction in Ganoderma to go out the spore oil polymer.
Character: for tan fat-soluble; Bitter in the mouth is little puckery.
Effect and purposes: Ganoderma has multiple pharmacological effect.The real elite of Ganoderma is that temperature is ensconced the lipid active substance in the Ganoderma spore.
1, ganoderan (Ganud ermal uci dum Polysacharide)
Structural formula:
The ganoderan structure
Ganoderan has been separated to kind more than 200 at present, and wherein major part is divided into α-glucosan, and minority is B-glucosan, polysaccharide chain is made of three strands of monosaccharide chains, be the three-dimensional formations of a kind of helical form, its three-dimensional configuration is similar with DNA, RNA, the hundreds of ten minutes etc. that arrive of molecular weight.Ganoderan has pharmacologically active widely, and energy human body immunity improving power improves body's hypoxia tolerance, eliminates free radical, suppresses tumor, radioprotective, improves liver, bone marrow, blood synthetic DNA, RNA, protein ability, slow down aging etc.
2, triterpenoid compound (triterpenes)
Structural formula:
Ganoderma triterpenoids compound structure type
Triterpenoid (triterpenes) has than fat-solubility, molecular weight is 400 ~ 600, and main component is Ganodenic acid (ganoderic acid), A ~ M (Ma ~ Mg), Ganoderma-22-olefin(e) acid (ganoaerinicacid), a ~ d, the single acid of Ganoderma (ganodermic acid) JaJbN, O, PZ, Ganoderma lucidum (Leyss. Ex Fr.) Karst. acid (lucidenic acid A), A ~ M, Ganoderma spore acid A (ganosporeni cacidA), ganoderol (ganoderiol) A ~ 1, ganoderal A, B Ganoderma lucidum (Leyss. Ex Fr.) Karst. spore lactone A, B, ergosterol-7,22-diene-3B, one pure linoleic acid fat, palmitic acid lipid ... Deng 112 kinds.Ganoderma triterpenoids has multiple pharmacological effect, as protect the liver, blood pressure lowering, inhibition HIV effect, 2000, the scholar of Japan folic hill medical university report had cytotoxic activity from the triterpenoid that Ganoderma spore extracts to Meth-A and LLC tumor cell, can kill such tumor cell.
The Main Ingredients and Appearance of Ganoderma is polysaccharide, triterpene.Studies show that in a large number polysaccharide has the effect that suppresses tumor, is to mediate the reinforcement host immune function and suppress tumor indirectly by the host.And Ganoderma triterpenoids has cytotoxic activity, can directly kill kinds of tumor cells.
Three, the relation of coenzyme Q10 and ganoderan, triterpene
The content of introducing from above is seen; the effect of coenzyme Q10 and Ganoderma spore oil and purposes have many similarities; consistent effect is being arranged aspect immunity, the liver protecting, inhibition tumor, resisting fatigue and the slow down aging improving, as joining any use and can increase treatment, health care and preventive effect.Studies show that coenzyme Q10 and Ganoderma triterpenoids be the mutual conspiracy relation of tool also, mainly show as coenzyme Q10 and can impel with the biosynthesis of Ganoderma triterpenoids and activate the activity of Ganoderma triterpenoids, make it the ability that in cytoactive, has more.
Four, compound benefit
1, treatment, health care and prophylactic function have been expanded.Though coenzyme Q10 is more intense in the effect of enhance immunity system aspects, to the too late Ganoderma triterpenoids in tumor cytotoxicity power aspect.Become compound after, just producing effect more aspect the special evaporation capacity of control tumor cell.
2, coenzyme Q10 can increase the biosynthesis ability of ganoderan, triterpene, certainly ganoderan, triterpene are not by biosynthesis, but coenzyme Q10 is to ganoderan, the active raising of triterpene aspect, no matter is ectogenic or is that synthetic voluntarily effect is identical.Like this, ganoderan, triterpene are having the effect behind the coenzyme Q10 more comprehensive.Resemble brave soldier and fight the enemy afield, competent reserve assistance strength has been arranged, it is violent more that food for powder is killed more, till to the last winning.
3, coenzyme Q10 is dissolved in Ganoderma spore oil, especially becomes easier in the Ganoderma spore oil preparation and is absorbed by the body, and has also increased the bioavailability of coenzyme Q10 simultaneously.
Clinical drug of the present invention uses the result to show to have following advantage and curative effect:
Implement 1:
Coenzyme Q10 (Coenzyme Q10 Capsules) 14.3%
Polysaccharide (Ganud ermal uci dum Polysacharide) 31.7%
Triterpenes (triterpenes) 54%
Production method:
With coenzyme Q10, Ganoderma spore wet goods source material fine powder oil, be mixed with into soft capsule and get final product.
Implement 2:
Coenzyme Q10 (Coenzyme Q10 Capsules) 20%
Polysaccharide (Ganud ermal uci dum Polysacharide) 35%
Triterpenes (triterpenes) 45%
Production method:
With coenzyme Q10, Ganoderma spore wet goods source material fine powder oil, be mixed with into soft capsule and get final product.
Implement 3:
Coenzyme Q10 (Coenzyme Q10 Capsules) 25.0%
Polysaccharide (Ganud ermal uci dum Polysacharide) 22.5%
Triterpenes (triterpenes) 52.5%
Production method:
With raw material fine powder oil such as coenzyme Q10, polysaccharide, triterpenes, be mixed with into soft capsule and get final product.
Claims (2)
1. a combination drug that suppresses tumor is characterized in that, is to make (consumption is weight percentage) by the raw material of following proportion:
Coenzyme Q10 (Coenzyme Q10 Capsules) 8-20%
Polysaccharide (Ganud ermal uci dum Polysacharide) 10-35%
Triterpenes (triterpenes) 25-54%.
2. according to claim, described product are made medicine processing method of the present invention with above-mentioned each component, it is characterized in that:
(a) adopt semi-synthesis method to be processed into the coenzyme Q10 raw material;
(b) adopt the extraction of microwave low-temperature solvent to be processed into Ganoderma spore oil polysaccharide raw material;
(c) adopt the extraction of microwave low-temperature solvent to be processed into Ganoderma spore oil triterpenes raw material;
(d) with after above-mentioned raw materials fine powder, the spore oil mixing, be prepared into soft capsule.The combination drug soft capsule that can promptly suppress tumor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100765910A CN101371857A (en) | 2007-08-24 | 2007-08-24 | Composite medicament for inhibiting tumor and preparation method thereof as well as technique field |
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| CNA2007100765910A CN101371857A (en) | 2007-08-24 | 2007-08-24 | Composite medicament for inhibiting tumor and preparation method thereof as well as technique field |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586902A (en) * | 2013-10-31 | 2015-05-06 | 江苏学府生物工程有限公司 | A method of producing cordyceps militaris-ganoderma spore powder soft capsule by a supercritical technique |
| CN106692215A (en) * | 2017-03-03 | 2017-05-24 | 广东仁源生物科技有限公司 | Application of coenzyme Q10 combined ganoderma lucidum spore oil in preparation of antiatherosclerotic drugs |
| CN110408590A (en) * | 2019-09-06 | 2019-11-05 | 遵义医学院附属医院 | A kind of induction method and application of the human mesenchymal stem cell to osteoblast differentiation |
-
2007
- 2007-08-24 CN CNA2007100765910A patent/CN101371857A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104586902A (en) * | 2013-10-31 | 2015-05-06 | 江苏学府生物工程有限公司 | A method of producing cordyceps militaris-ganoderma spore powder soft capsule by a supercritical technique |
| CN106692215A (en) * | 2017-03-03 | 2017-05-24 | 广东仁源生物科技有限公司 | Application of coenzyme Q10 combined ganoderma lucidum spore oil in preparation of antiatherosclerotic drugs |
| CN110408590A (en) * | 2019-09-06 | 2019-11-05 | 遵义医学院附属医院 | A kind of induction method and application of the human mesenchymal stem cell to osteoblast differentiation |
| CN110408590B (en) * | 2019-09-06 | 2022-10-25 | 遵义医学院附属医院 | Method for inducing differentiation of human mesenchymal stem cells into osteoblasts and application thereof |
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Open date: 20090225 |