CN101357151A - Method for extracting total lactones from auckandia root and quality control method thereof - Google Patents
Method for extracting total lactones from auckandia root and quality control method thereof Download PDFInfo
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- CN101357151A CN101357151A CNA2008101464050A CN200810146405A CN101357151A CN 101357151 A CN101357151 A CN 101357151A CN A2008101464050 A CNA2008101464050 A CN A2008101464050A CN 200810146405 A CN200810146405 A CN 200810146405A CN 101357151 A CN101357151 A CN 101357151A
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Abstract
The invention provides a method for extracting total lactones from costus root by a CO2 supercritical extraction technology and a quality control method thereof. In the invention, the extraction pressure is 10-25MPa, the extraction temperature is 25-40 DEG C, and the extraction time is 2-4 hours, and analysis is carried out at an air pressure of 5-6MPa and a temperature of 34-40 DEG C. The method solves the disadvantages of the conventional extraction process that the operating temperature is relatively high, thermosensitive substance is easy to be oxidized and dissipated, high boiling point substance is not easy to be fully extracted, the yield is low, etc. In an extract of the invention, the content of dehydrocostus lactone is higher than 20%, and the total content of costundide and dehydrocostus lactone is higher than 50%.
Description
Technical field
The present invention relates to a kind of preparation method of from the Chinese medicine Radix Aucklandiae, extracting the Radix Aucklandiae total lactones be used for the treatment of motility disturbances of the stomach, and the method for quality control of the extract that makes with this method.Belong to medical technical field.
Background technology
Gastric motility disorder is common clinical, frequently-occurring disease.In China, along with social development, the change of circumstances, the change of population structure and people life style, main because of smoking, drink, gastropathy sickness rate that nervous, medicine irritation etc. causes increases gradually.On the other hand by social pressure, the food former thereby that produce of working does not have regularly, eats stomach discomfort stomach function regulating dynamical problem that irregular grade causes in constantly increasing yet.
For a long time, the medicine of treatment gastric motility disorder is played protagonist by chemical medicine always, and the side effect of chemical medicine then is the difficult problem of the world of medicine.Clinical medicine commonly used has four at present: paspertin metoclopramide, motilium, the upright and erythromycin of Xisha ratio, but because their clinical side effects, all can not be as the medicine for stomach dynamic first-selection.Diseases such as Chinese medicine " acid regurgitation ", " noisy ", " stomach painful abdominal mass ", " thoracic obstruction " have had history in several thousand and have accumulated rich experience.Facts have proved that Chinese medicine is tall and erect effective in cure and safety economy to diseases such as gastric motility disorders, relapse rate also is lower than western modern medicine.Influence the Chinese medicine of gastrointestinal motility, belong to the QI regulating medicine of traditional Chinese medical science traditional classification method mostly, generally be divided into three classes, digestive tract power promotes, digestive tract power suppresses and digestive tract power is had two-way or multidirectional regulating action three major types.The Radix Aucklandiae belongs to the 3rd class, and digestive tract power is had dual regulation.
The Radix Aucklandiae is the dry root of feverfew Radix Aucklandiae Aucklandia lappa Decne.Be the medicine commonly used of treatment gastrointestinal disease, the Sheng Nong's herbal classic is classified as top grade.Costunolide and dehydrocostuslactone are the main components of volatile oil of Radix Aucklandiae, and they have relaxing smooth muscle and spasmolysis, are the main active of the Radix Aucklandiae, also are the leading indicator of Radix Aucklandiae quality control.
Radix Aucklandiae total lactones is to extract the lactone composition that obtains from the Chinese medicine Radix Aucklandiae, and main component wherein is volatile oil such as costunolide and dehydrocostuslactone.
The extracting method of volatile oil is many at present, and the extraction process by water yield is lower; Alcohol extraction process can cause volatile oil and loss of effective components bigger, and introduces impurity easily; Microwave extraction method and ultrasonic extraction only limit to the laboratory small-scale operations at present, are not suitable for big production.The CO that the present invention adopts
2Plurality of advantages such as the supercritical extraction technique rate of extraction is fast, does not have residual organic solvent, and flow process is simple, and is easy to operate.
Summary of the invention
The purpose of this invention is to provide a kind of employing CO
2Supercritical extraction technique extracts the method and the method for quality control thereof of total lactone from the Radix Aucklandiae.
Employing CO provided by the invention
2Supercritical extraction technique extracts total lactone from the Radix Aucklandiae method comprises the steps:
(a) pre-treatment of Radix Aucklandiae medical material;
(b) adopt CO
2Supercritical extraction technique extracts total lactone from the Radix Aucklandiae; Extract brownish red or the translucent grease of pale brown color, low temperature (4 ℃) is placed with a large amount of crystalline solids and separates out;
(c) separate: take out extract, the centrifugal moisture of removing gets the product Radix Aucklandiae total lactones; Calculate extraction ratio, and measure the content of total lactone in the Radix Aucklandiae total lactones;
Following method is preferably adopted in the pre-treatment of step (a) Radix Aucklandiae medical material:
Get Radix Aucklandiae medical material, drying is 6 hours under 40～45 ℃, is ground into coarse powder.
Step (b) adopts CO
2Supercritical extraction technique extracts total lactone and adopts following method from the Radix Aucklandiae:
Step (b) is with the Radix Aucklandiae medicinal material coarse powder CO that packs into
2In the supercritical extraction jar, be 10～25Mpa with pressure,
Temperature is that 25～40 ℃ condition extracted 2～4 hours, is 5-6Mpa with pressure, and temperature is 35-40 ℃ and resolves.
Step (b) most preferred technique scheme is: in extracting pressure is that 20Mpa, temperature are to extract 2 hours under 30 ℃ of conditions; At pressure is 6Mpa, and temperature is to resolve under 35 ℃ of conditions.
Radix Aucklandiae medical material can be selected Radix Aucklandiae root for use.
The Radix Aucklandiae total lactones product of the present invention of treatment effective dose adds pharmaceutically acceptable carrier, can be prepared into any dosage form.
Radix Aucklandiae total lactones product of the present invention can be used for treatment or prevention motility disturbances of the stomach, promoting the circulation of QI to relieve pain, and strengthening the spleen to promote digestion has good therapeutic effect.
It is higher to the invention solves in the traditional extraction technique operative temperature, the easy oxidation loss of heat-sensitive substance, and high boiling substance is difficult for proposing fully, shortcoming such as low under the yield.Adopt high performance liquid chromatography that the effective ingredient of Radix Aucklandiae total lactones is measured, in the extract of the present invention, dehydrocostuslactone content is higher than 20%; Costunolide and dehydrocostuslactone content sum are higher than 50%.
The present invention also provides the method for quality control of the extract that this method makes.Specific as follows:
[character] this product is in brownish red or pale brown color oily liquids or brownish red or the pale brown color oily liquids suspension crystalline particle to be arranged.Gas perfume (or spice) is special, bitter in the mouth.Insoluble in water, methanol, ethanol, molten in the ethyl acetate part omitted, very easily molten in acetone, chloroform, ether.
[discriminating] precision takes by weighing the about 30mg of this product, puts in the 10ml measuring bottle, and adding diethyl ether makes dissolving, adds diethyl ether to scale, shakes up, as need testing solution.Other takes by weighing Radix Aucklandiae medical material 10g, soaks 40 minutes with the 20ml ether, filters, and gets filtrate volatilizing, and adds the 5ml ether dissolution, in contrast product solution (1).Precision takes by weighing costunolide reference substance 5mg and the about 5mg of dehydrocostuslactone reference substance in addition, puts in the 5ml measuring bottle, adds ethyl acetate 0.5ml and makes dissolving, adds methanol again to scale, shakes up, in contrast product solution (2).Draw each 5 μ l of above-mentioned solution according to thin layer chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 B), put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with chloroform-cyclohexane extraction (5: 1) is developing solvent, launch, take out, dry, spray is with 1% vanillin concentrated sulphuric acid, and it is clear to be heated to speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
[assay] measured according to high performance liquid chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 D).
Chromatographic condition and system suitability octadecylsilane chemically bonded silica are filler, and methanol-water (75: 25) is a mobile phase; The detection wavelength is 225nm.Number of theoretical plate is not less than 4000 by the dehydrocostuslactone peak.
The preparation precision of reference substance solution takes by weighing about 10mg of costunolide reference substance (available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute) and the about 10mg of dehydrocostuslactone reference substance (available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute), put respectively in the 100ml measuring bottle, add ethyl acetate 3ml and make dissolving, add methanol to scale, shake up; Precision is measured above-mentioned costunolide solution 3ml respectively, and dehydrocostuslactone solution 5ml puts in the 10ml measuring bottle, adds methanol to scale, shakes up, promptly.
The preparation precision of need testing solution takes by weighing the about 100mg of this product, puts in the 100ml measuring bottle, adds ethyl acetate 20ml and makes dissolving, adds methanol to scale, shakes up, and filters, and precision is measured subsequent filtrate 3ml, puts in the 25ml measuring bottle, adds methanol to scale, shakes up, promptly.
Accurate respectively reference substance solution and each the 10 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, and the record peak area is by external standard method, with calculated by peak area, promptly.
This product contains dehydrocostuslactone (C
15H
20O
2) must not be less than 20%.Containing costunolide and dehydrocostuslactone sum is Radix Aucklandiae total lactones, must not be less than 50%.
The specific embodiment
Crude drug source: medical material is available from Shenyang City's medical material purchasing and supply station, and its former plant is the dry root of chrysanthemum sample plant Radix Aucklandiae Auchlandia lappa Dence., originates in provinces such as Yunnan, Sichuan.
The preparation of medical material: took by weighing 40～45 ℃ of dryings of medical material 6 hours, it is standby to be ground into coarse powder.
Embodiment 1:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 25 ℃ in extraction temperature, and extracting pressure is 10Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 5Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.32%, and total lactone amount is 60.2%, and dehydrocostuslactone content is 31.9%.
Embodiment 2:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 35 ℃ in extraction temperature, and extracting pressure is 10Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 4 hours is 6Mpa at pressure, and temperature is to resolve under 40 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 2.86%, and total lactone amount is 58.1%., dehydrocostuslactone content is 30.8%.
Embodiment 3:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 40 ℃ in extraction temperature, and extracting pressure is 25Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 3 hours is 6Mpa at pressure, and temperature is to resolve under 40 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 2.60%, and total lactone amount is 54.2%, and dehydrocostuslactone content is 28.7%.
Embodiment 4:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 25 ℃ in extraction temperature, and extracting pressure is 15Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.61%, and total lactone amount is 72.6%, and dehydrocostuslactone content is 38.5%.
Embodiment 5:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 30 ℃ in extraction temperature, and extracting pressure is 25Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.45%, and total lactone amount is 71.2%, and dehydrocostuslactone content is 37.8%.
Embodiment 6:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 40 ℃ in extraction temperature, and extracting pressure is 15Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.30%, and total lactone amount is 66.5%, and dehydrocostuslactone content is 35.2%.
Embodiment 7:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 20 ℃ in extraction temperature, and extracting pressure is 25Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.74%, and total lactone amount is 76.2%, and dehydrocostuslactone content is 40.3%.
Embodiment 8:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 35 ℃ in extraction temperature, and extracting pressure is 20Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.60%, and total lactone amount is 74.3%, and dehydrocostuslactone content is 39.3%.
Embodiment 9:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 40 ℃ in extraction temperature, and extracting pressure is 20Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.41%, and total lactone amount is 68.1%, and dehydrocostuslactone content is 36.1%.
Embodiment 10:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 30 ℃ in extraction temperature, and extracting pressure is 20Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.85%, and total lactone amount is 79.2%, and dehydrocostuslactone content is 42.0%.
Embodiment 11:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 35 ℃ in extraction temperature, and extracting pressure is 25Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.73%, and total lactone amount is 76.2%, and dehydrocostuslactone content is 40.4%.
Embodiment 12:
Get Radix Aucklandiae medicinal material coarse powder 500g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 30 ℃ in extraction temperature, and extracting pressure is 23Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.65%, and total lactone amount is 70.2%, and dehydrocostuslactone content is 37.2%.
Embodiment 13:
Get Radix Aucklandiae medicinal material coarse powder 150g and place extraction apparatus, use CO
2Get rid of air in the extractor, regulate extractor, separator pressure, temperature is 30 ℃ in extraction temperature, and extracting pressure is 20Mpa, CO
2Flow velocity is under 21～25L/h condition, and continuous extraction 2 hours is 6Mpa at pressure, and temperature is to resolve under 35 ℃ of conditions, extracts brownish red or the translucent grease of pale brown color, takes out extract from separator, and the centrifugal moisture of removing obtains the product Radix Aucklandiae total lactones.Extraction ratio is 3.86%, and total lactone amount is 79.5%, and dehydrocostuslactone content is 42.1%.
Annotate:
Used extraction apparatus is: the model of Huaan, Nantong supercritical extraction equipment company limited production is the HA221-50-60 extractor, the key technical indexes: the highest extracting pressure: 50Mpa; Extractor volume: 5L, 1L; Extraction temperature: room temperature-75 degree; Maximum stream flow: 50L/h, adjustable.
Above embodiment, or not within the spirit and principles in the present invention not all only for the purpose of description in order to restriction the present invention, any modification of being done, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (13)
1, a kind of employing CO
2Supercritical extraction technique extracts the method for total lactone from the Radix Aucklandiae, it is characterized in that comprising the steps:
(b) adopt CO
2Supercritical extraction technique extracts total lactone from the Radix Aucklandiae.
2, method according to claim 1 is characterized in that in the described step (b), extracting pressure is 10～25Mpa, and temperature is 25～40 ℃.
3, method according to claim 1 and 2 is characterized in that in the described step (b), parsing pressure is 5-6Mpa, and resolution temperature is 35 ℃-40 ℃.
4, method according to claim 3 is characterized in that in the described step (b), the extraction time is 2-4 hour.
5, method according to claim 1 and 2 is characterized in that described step (b) is: in extracting pressure is that 20Mpa, temperature are to extract 2 hours under 30 ℃ of conditions; At pressure is 6Mpa, and temperature is to resolve under 35 ℃ of conditions.
6, method according to claim 5 is characterized in that step (b) also comprises the steps: before
(a) pre-treatment of Radix Aucklandiae medical material: get Radix Aucklandiae medical material, drying is 6 hours under 40～45 ℃, is ground into coarse powder.
7, method according to claim 6 is characterized in that described Radix Aucklandiae medical material is a Radix Aucklandiae root.
8, method according to claim 6, it is characterized in that step (b) comprises the steps: also that afterwards (c) separates: take out extract, the centrifugal moisture of removing gets the product Radix Aucklandiae total lactones.
9, method according to claim 8 is characterized in that in the product of step (c), and the total lactone content of this perfume is more than 50%.
10, method according to claim 9 is characterized in that in the product of step (c), dehydrocostuslactone content is higher than 20%.
11, a kind of Banksia rose total lactone medicament composition is characterized in that wherein containing each products therefrom and pharmaceutically acceptable carrier among the claim 1-10.
12, the application of each products therefrom in the medicine of preparation treatment or prevention motility disturbances of the stomach among the claim 1-11.
13, a kind of CO
2Supercritical extraction technique extracts the method for quality control of total lactone from the Radix Aucklandiae, comprising:
This product is the oily liquids that the dry root of feverfew Radix Aucklandiae Aucklandia Lappa Decne. obtains through carbon dioxide supercritical fluid extraction;
Character: this product is in brownish red or pale brown color oily liquids or brownish red or the pale brown color oily liquids suspension crystalline particle to be arranged; Gas perfume (or spice) is special, bitter in the mouth; Insoluble in water, methanol, ethanol, molten in the ethyl acetate part omitted, very easily molten in acetone, chloroform, ether;
Differentiate: precision takes by weighing the about 30mg of this product, puts in the 10ml measuring bottle, and adding diethyl ether makes dissolving, adds diethyl ether to scale, shakes up, as need testing solution.Other takes by weighing Radix Aucklandiae medical material 10g, soaks 40 minutes with the 20ml ether, filters, and gets filtrate volatilizing, and adds the 5ml ether dissolution, and product solution 1 in contrast; Precision takes by weighing costunolide reference substance 5mg and the about 5mg of dehydrocostuslactone reference substance in addition, puts in the 5ml measuring bottle, adds ethyl acetate 0.5ml and makes dissolving, adds methanol again to scale, shakes up, and product solution 2 in contrast; Draw each 5 μ l of above-mentioned solution according to the thin layer chromatography among an appendix VI of Chinese Pharmacopoeia version in 2005 B, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with chloroform-cyclohexane extraction of 5: 1 was developing solvent, launch, take out, dry, spray is with 1% vanillin concentrated sulphuric acid, and it is clear to be heated to speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color;
Assay: according to the high effective liquid chromatography for measuring of an appendix VI of Chinese Pharmacopoeia version in 2005 D;
Chromatographic condition and system suitability: with octadecylsilane chemically bonded silica is filler, and methanol-water (75: 25) is a mobile phase; The detection wavelength is 225nm.Number of theoretical plate is not less than 4000 by the dehydrocostuslactone peak;
The preparation of reference substance solution: precision takes by weighing about 10mg of costunolide reference substance and the about 10mg of dehydrocostuslactone reference substance, puts respectively in the 100ml measuring bottle, adds ethyl acetate 3ml and makes dissolving, adds methanol to scale, shakes up; Precision is measured above-mentioned costunolide solution 3ml respectively, and dehydrocostuslactone solution 5ml puts in the 10ml measuring bottle, adds methanol to scale, shakes up, promptly;
The preparation of need testing solution: precision takes by weighing the about 100mg of this product, puts in the 100ml measuring bottle, adds ethyl acetate 20ml and makes dissolving, adds methanol to scale, shakes up, and filters, and precision is measured subsequent filtrate 3ml, puts in the 25ml measuring bottle, adds methanol to scale, shakes up, promptly;
Algoscopy: accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing, inject chromatograph of liquid, the record peak area is by external standard method, with calculated by peak area, promptly;
This product contains dehydrocostuslactone (C
15H
20O
2) must not be less than 20%; Containing costunolide and dehydrocostuslactone sum is Radix Aucklandiae total lactones, must not be less than 50%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101464050A CN101357151A (en) | 2008-08-28 | 2008-08-28 | Method for extracting total lactones from auckandia root and quality control method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101464050A CN101357151A (en) | 2008-08-28 | 2008-08-28 | Method for extracting total lactones from auckandia root and quality control method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101357151A true CN101357151A (en) | 2009-02-04 |
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|---|---|---|---|
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648927A (en) * | 2011-02-25 | 2012-08-29 | 苏州宝泽堂医药科技有限公司 | Production technology of petasites lactone compound |
| CN102978005A (en) * | 2012-12-06 | 2013-03-20 | 重庆市日用化学工业研究所 | Method for extracting radix saussureae lappae essential oil by supercritical carbon dioxide |
| CN103706145A (en) * | 2012-10-01 | 2014-04-09 | 青岛科技大学 | Subcritical CO2 based method used for low-temperature removing of residual solvents from thermal sensitive solid medicines |
-
2008
- 2008-08-28 CN CNA2008101464050A patent/CN101357151A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648927A (en) * | 2011-02-25 | 2012-08-29 | 苏州宝泽堂医药科技有限公司 | Production technology of petasites lactone compound |
| CN103706145A (en) * | 2012-10-01 | 2014-04-09 | 青岛科技大学 | Subcritical CO2 based method used for low-temperature removing of residual solvents from thermal sensitive solid medicines |
| CN103706145B (en) * | 2012-10-01 | 2016-08-10 | 青岛科技大学 | A kind of based on subcritical CO2the technique of residual solvent in low temperature removing thermal sensitivity solid drugs |
| CN102978005A (en) * | 2012-12-06 | 2013-03-20 | 重庆市日用化学工业研究所 | Method for extracting radix saussureae lappae essential oil by supercritical carbon dioxide |
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Application publication date: 20090204 |