CN101327339A - Silk fibroin tissue engineering spinal cord damnification repair material and use - Google Patents
Silk fibroin tissue engineering spinal cord damnification repair material and use Download PDFInfo
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- CN101327339A CN101327339A CNA2008100220167A CN200810022016A CN101327339A CN 101327339 A CN101327339 A CN 101327339A CN A2008100220167 A CNA2008100220167 A CN A2008100220167A CN 200810022016 A CN200810022016 A CN 200810022016A CN 101327339 A CN101327339 A CN 101327339A
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- spinal cord
- silk fibroin
- tissue engineering
- catheter
- repair material
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Abstract
The invention discloses a material used for repairing spinal cord injured in the silk fibroin tissue engineering and an application thereof. The product includes an artificial spinal catheter which is a catheter containing silk fibroin fiber frame. The invention is used for preparing a material for repairing spinal cord injured. The product of the invention adopts silk fibroin of high purity as a raw material. As a naturally degradable material, the material disclosed by the invention has good biocompatibility to human body. The product does does not contain materials with exogenous toxic and side effects caused in preparation technics. The catheter wall has a three-dimensional structure with plentiful micropores, which provides nutrients for the growth of nerve cells, with good effect; besides, the three-dimensional structure of the catheter wall provides necessary induction and growing space.
Description
Technical field:
The present invention relates to a kind of repair of spinal cord injury material and purposes.
Background technology:
Quickening along with social modernization's process and people's rhythm of life, more vehicle accident, the high-altitude incidents such as accident, earthquake, war of falling, move inevitably can appear, simultaneously frequent local war and natural disasters such as incident of violence and earthquake all can cause the patient of spinal cord injury to increase day by day.Though the incidence rate various countries of spinal cord injury do not have accurate data, generally estimate in 20～60 people/1,000,000 people/years.Spinal cord injury is the central nervous system injury that a kind of disability rate is high, consequence is very serious, the damage location pilosity is born in the spinal cord of ceruical uertebra part and lumbar segment, can cause the forfeiture of the following motion in damage plane, sensory function, bring chronic pain simultaneously, even mental maladjustment.Because spinal cord injury higher incidence and disability rate still do not have effective treatment way at present, be mainly in juvenile age level again to the prime of life, this brings heavy economy and mental burden must for patient, family and entire society.When running into patients with spinal cord injury clinically at present, only be confined to use some medicines and slow down the paralysis degree in conjunction with a spot of naturopathy means.Therefore, the treatment of spinal cord injury is one of global problem of puzzlement medical circle always.
People repay examination and make up man-made support material and/or auxilliary seed addition cell or cytokine, repair spinal cord injury, wherein material configuration is designed to sponge glue (or even fluent material implant damage place allows it be frozen into jelly) or fibrous or many microtubules shape or multitube shape or single tube shape or the like, and related material has collagen protein, gelatin, chitosan.But because these man-made support materials still exist many problems not fully up to expectations, influenced its repairing effect, therefore will make them can really reach the clinical practice purpose, also needed to make very big effort.
Summary of the invention:
The object of the present invention is to provide a kind of and human body that excellent biological compatibility is arranged and have excellent biodegradability can silk fibroin tissue engineering spinal cord damnification repair material and purposes.
Technical solution of the present invention is:
A kind of silk fibroin tissue engineering spinal cord damnification repair material is characterized in that: comprise artificial spinal catheter, artificial spinal catheter is the conduit that contains the fibroin fiber skeleton.
The cytokine that on artificial spinal catheter, contains the tool therapeutical effect.
The cytokine of described tool therapeutical effect is one or more in nerve growth factor, neurotrophic factor-3, Brain Derived Neurotrophic Factor, glial cell line-derived neurotrophic factor, the medicine.
Contain seed cell in the artificial spinal catheter.
Seed cell is one or more in bone marrow stroma stem cell, neural stem cell, Schwann cell, Olfactory essheathing cell, the embryonic stem cell.
The application of a kind of silk fibroin tissue engineering spinal cord damnification repair material in preparation repair of spinal cord injury material.
Product material therefor of the present invention is the high-purity natural silk, and it is the natural degradable material, with human body excellent biological compatibility is arranged.The product that makes does not contain because the exogenous toxicity, side effect material that preparation technology brings into.Duct wall has the three dimensional structure that enriches micropore, and for the conveying of the required nutrient substance of nerve growth process provides necessary approach, result of use is good, for the growth of neurocyte provides necessary inducing action and necessary growing space.
The invention will be further described below in conjunction with embodiment.
The specific embodiment:
Structure and shape according to human body and animal spinal cord, the mould that main up fibre bundle and descending fibre bundle subregion form are complementary in design and structure and gray nucleus and white matter, the white matter, prepare the tissue engineering spinal cord conduit with fibroin albumen, make the conduit that contains the fibroin fiber skeleton.Concrete grammar can adopt Chinese patent CN100382772 to make the method that contains the fibroin albumen conduit.
Further utilize combination chemistry or physics, the cytokine or the medicine that will have therapeutical effect are fixed to the fibroin albumen spinal catheter.The cytokine of described tool therapeutical effect is one or more in nerve growth factor, neurotrophic factor-3, Brain Derived Neurotrophic Factor, glial cell line-derived neurotrophic factor, the medicine.
Separate, cultivate seed cell, then seed cell and the artificial spinal catheter of fibroin albumen are united cultivation, form tissue engineering spinal cord, wherein the type of seed cell comprises: bone marrow stroma stem cell, neural stem cell, Schwann cell, Olfactory essheathing cell, embryonic stem cell etc., can the separate cell kind use, also can unite use by several cells.
Claims (6)
1, a kind of silk fibroin tissue engineering spinal cord damnification repair material is characterized in that: comprise artificial spinal catheter, artificial spinal catheter is the conduit that contains the fibroin fiber skeleton.
2, silk fibroin tissue engineering spinal cord damnification repair material according to claim 1 is characterized in that: the cytokine that contains the tool therapeutical effect on artificial spinal catheter.
3, silk fibroin tissue engineering spinal cord damnification repair material according to claim 2 is characterized in that: the cytokine of described tool therapeutical effect is one or more in nerve growth factor, neurotrophic factor-3, Brain Derived Neurotrophic Factor, glial cell line-derived neurotrophic factor, the medicine.
4, according to claim 1,2 or 3 described silk fibroin tissue engineering spinal cord damnification repair materials, it is characterized in that: contain seed cell in the artificial spinal catheter.
5, silk fibroin tissue engineering spinal cord damnification repair material according to claim 4 is characterized in that: seed cell is one or more in bone marrow stroma stem cell, neural stem cell, Schwann cell, Olfactory essheathing cell, the embryonic stem cell.
6, the application of a kind of silk fibroin tissue engineering spinal cord damnification repair material in preparation repair of spinal cord injury material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100220167A CN101327339A (en) | 2008-06-24 | 2008-06-24 | Silk fibroin tissue engineering spinal cord damnification repair material and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100220167A CN101327339A (en) | 2008-06-24 | 2008-06-24 | Silk fibroin tissue engineering spinal cord damnification repair material and use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101327339A true CN101327339A (en) | 2008-12-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100220167A Pending CN101327339A (en) | 2008-06-24 | 2008-06-24 | Silk fibroin tissue engineering spinal cord damnification repair material and use |
Country Status (1)
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| CN (1) | CN101327339A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102727936A (en) * | 2012-06-20 | 2012-10-17 | 中山大学 | Construction method of sustained-release NT-3 gelatin sponge cylindrical stent used for repairing spinal cord injuries |
| CN106983914A (en) * | 2017-05-12 | 2017-07-28 | 南通市第人民医院 | Partition type chitosan gelatin silk microfibre spinal cord rack and preparation method thereof |
| CN110665059A (en) * | 2019-10-17 | 2020-01-10 | 苏州大学 | Tissue engineering nerve transplant and preparation method thereof |
| CN111603608A (en) * | 2020-05-15 | 2020-09-01 | 南通大学 | Pullulan-hyaluronic acid-microfiber spinal cord scaffold and preparation method thereof |
-
2008
- 2008-06-24 CN CNA2008100220167A patent/CN101327339A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102727936A (en) * | 2012-06-20 | 2012-10-17 | 中山大学 | Construction method of sustained-release NT-3 gelatin sponge cylindrical stent used for repairing spinal cord injuries |
| CN106983914A (en) * | 2017-05-12 | 2017-07-28 | 南通市第人民医院 | Partition type chitosan gelatin silk microfibre spinal cord rack and preparation method thereof |
| CN106983914B (en) * | 2017-05-12 | 2019-09-06 | 南通市第一人民医院 | Partition type chitosan-gelatin-silk microfibre spinal cord rack and preparation method thereof |
| CN110665059A (en) * | 2019-10-17 | 2020-01-10 | 苏州大学 | Tissue engineering nerve transplant and preparation method thereof |
| CN110665059B (en) * | 2019-10-17 | 2021-09-28 | 苏州大学 | Tissue engineering nerve transplant and preparation method thereof |
| CN111603608A (en) * | 2020-05-15 | 2020-09-01 | 南通大学 | Pullulan-hyaluronic acid-microfiber spinal cord scaffold and preparation method thereof |
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Application publication date: 20081224 |