CN101314500A - Microorganism colonization method - Google Patents

Microorganism colonization method Download PDF

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Publication number
CN101314500A
CN101314500A CNA2007101057882A CN200710105788A CN101314500A CN 101314500 A CN101314500 A CN 101314500A CN A2007101057882 A CNA2007101057882 A CN A2007101057882A CN 200710105788 A CN200710105788 A CN 200710105788A CN 101314500 A CN101314500 A CN 101314500A
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CN
China
Prior art keywords
magnetic
microorganism
microorganisms
packing
filler
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Pending
Application number
CNA2007101057882A
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Chinese (zh)
Inventor
戴松林
罗晓虹
程江
黄奂彦
卢志刚
刘可旋
皮丕辉
文秀芳
杨卓如
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DONGGUAN INSTITUTE OF ENVIRONMENTAL SCIENCE
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DONGGUAN INSTITUTE OF ENVIRONMENTAL SCIENCE
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Priority to CNA2007101057882A priority Critical patent/CN101314500A/en
Publication of CN101314500A publication Critical patent/CN101314500A/en
Pending legal-status Critical Current

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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

Abstract

The invention discloses a method for hanging a film by microorganisms. The method comprises the following steps of: placing magnetic packing in a bio-packing water treatment device; adding ferrite magnetic powder or ferromagnetic particulates into activated sludge for domestication to allow part of the microorganisms to be attached on the surface of the magnetic powder to form activated magnetic powder sludge; adding the activated magnetic powder sludge into the bio-packing water treatment device; aerating air to allow mass magnetic powder carrying the microorganisms to be quickly attached on the surface of the magnetic packing under the actions of air impact and magnetic attraction of the packing, thereby realizing that the film is hung on the surface of the magnetic packing by the microorganisms. The process of hanging the film by the microorganisms can be promoted and accelerated by using the attraction between the magnetic packing and the magnetic powder-microorganism mixture; and the action of a low intensity field can induce the microbial activity and the enzymatic activity, and the microorganisms can better grow, propagate and succeed, thereby improving the water treatment efficiency. The method for hanging the film by the microorganisms is easily operated and controlled, has low cost, can be widely applied in the water treatment industry, in particular the aerobic biological film treatment for waste/sewage water.

Description

A kind of microorganism colonization method
Technical field
The present invention relates to water-treatment technology field, relate in particular to a kind of fast microbiological colonization method that biomembrance process is handled trade effluent or sanitary sewage that is used for.
Background technology
Microbial film is the core of biological water technology, and biomembranous quality is directly connected to the treatment effect of biological water treatment device.The key factor that influences microbial film formation (carrier surface forming biofilm property) mainly contains two aspects, the one, colonization method, the 2nd, the surface property of biologic packing material.Make short, the instant effect of the biofilm process time of microorganism on filler, key is: the microorganism of capacity must be arranged before the biofilm, and must adapt under the environment when waste water enters bio-reactor and grow, like this, in a single day microorganism is attracted on the filler, need not the adaptive phase and the ramp breeding; Next is when biofilm, between microorganism in the bacterium liquid and the solid phase filler stronger absorption, adhesive capacity is arranged, and adhere firmly.About the former principle and method, extensively used at present.
To back one factor, at present many about the report of biologic packing material modification aspect, comprise surface hydrophilic modification, biological affine modification and magnetization modification etc.Wherein, the magnetization modification filler is as a kind of novel biologic packing material, by in preparation filler process, adding magnetic or strong magnetic particle, and magnetize, make filler have weak magnetic, then filler is directly added bacterium liquid and biofilm can activate the certain micro-organisms activity, realize the application of magnetic effect in biological water treatment.But since the most of microbe magnetic responsiveness a little less than, biofilm speed is slow and the biofilm amount is few, adsorptive power is not strong between microorganism and the solid phase filler.
Summary of the invention
In order to overcome the defective that existing microorganism colonization method exists, the object of the present invention is to provide a kind of novel method of enhancement microbiological carrier surface forming biofilm property, the biofilm that not only can improve biologic packing material starts speed and biofilm amount, and can impact and the resistance to overload shock ability by the anti-aeration of reinforcing filler surface biological film.
The technical solution adopted for the present invention to solve the technical problems is: the present invention is based on back one factor, and by strengthening the adsorptive power between filler and the microorganism, to promote the biofilm of microorganism on filler, its microorganism colonization method comprises the steps:
(1) filler that will have magnetic is put into the biologic packing material water treatment device;
(2) adding ferromagnetic oxide powder or strong magnetic particle are tamed in active sludge, make the part microorganic adhesion on the magnetic surface, form activation magnetic mud;
(3) again the mud that is mixed with magnetic is joined in the biologic packing material water treatment device;
(4) bubbling air, under the magnetic attraction effect of the impact of air-flow and filler, a large amount of magnetics that carry microorganism will be attached to the magnetic fillers surface rapidly, thereby realize the microorganism colonization on magnetic fillers surface.
The preparation technology of activation magnetic mud: with mud is bacterial classification, adds wastewater/sewage and a small amount of nutritive substance, adds magnetic simultaneously, bubbling air, treat that microorganism and magnetic settle down after, remove supernatant liquid and add new wastewater/sewage, so repeatedly, at room temperature cultivate a couple of days, promptly.
The preparation technology of magnetic fillers: in polymer base material, add magnetic and coupling lubricant, use the injection moulding machine injection moulding; The gained filler is magnetized by magnet charger, can make it have nonvolatil magnetic field, promptly.
The invention has the beneficial effects as follows that the present invention utilizes the sucking action between magnetic fillers and the magnetic-microbial mixture to promote, accelerate microorganism colonization; Because the effect of low-intensity magnetic field can be induced microorganism active and enzymic activity, thereby be made microorganism growth and breeding, succession better, improve water treatment efficiency simultaneously.The control of this microorganism colonization method easy handling, with low cost, the aerobic biologic membrane that can be widely used in water treatment field, particularly wastewater/sewage is handled.
Embodiment
Microorganism colonization method of the present invention comprises the filler that has magnetic is put into the biologic packing material water treatment device; In mud, add iron oxide magnetic powder or strong magnetic particle, after aeration mixes, the part microorganic adhesion is arranged on the magnetic surface; Through cultivation after a while, when microbial reproduction acquires a certain degree, the active sludge that is mixed with magnetic is joined in the biologic packing material water treatment device; Bubbling air, under the impact and filler magnetic action of air-flow, the a large amount of magnetics that carry microorganism will be attached to filling surface rapidly, not only increased the filling surface microbial biomass, also strengthen the roughness of filling surface, increase the attachment point of microorganism, thereby promoted biomembranous quick formation, realized the microorganism colonization on magnetic fillers surface.
The preparation of activation magnetic mud: the mud with the municipal sewage treatment station is bacterial classification, adds the wastewater/sewage that needs processing in right amount, adds a small amount of nutritive substance such as glucose or KH again 2PO 4, add an amount of magnetic simultaneously; Bubbling air stops aeration after (1~2 day) at set intervals, microorganism and magnetic are settled down after, exclude supernatant liquid and add the new wastewater/sewage of as much, so at room temperature cultivate a couple of days repeatedly, promptly get and activate magnetic mud.
The preparation of magnetic fillers: in polymer base material, add a certain amount of magnetic and coupling lubricant, after the injection moulding machine injection moulding, is that magnet charger about 8000~15000 Gausses magnetizes with the gained filler by magneticstrength, can make it have nonvolatil magnetic field, average intensity is about 50~120 Gausses.
Described polymer base material can be that polyethylene or polypropylene or polyvinyl chloride or polyester are one kind of;
Described magnetic mainly is wherein one or more mixtures of Z 250, ferric oxide, iron monoxide, barium ferrite, the strong magnetic particle of rare earth such as neodymium iron boron;
Described coupling lubricant is the mixture of stearic acid, vegetable and animals oils, white mineral oil.
The optimum weight umber of above-mentioned raw materials consumption is as follows:
Polymer base material 78~95
Magnetic 5~20
Coupling lubricant 0.5~2
Microorganism colonization process of the present invention: choose several prepared magnetic fillers and place biological water treatment device, each filler can be with rope equidistant fixing (fixed), or loose and pile up (distributing) in the device.Then toward wherein adding prepared activation magnetic sludge suspension, make filler all be immersed in the suspension, afterwards with certain flow rate bubbling air, under gas shock and magnetic field force effect, the magnetic sludge that carries a large amount of microorganisms is attached to the magnetic fillers surface rapidly with magnetic, thereby filling surface microorganic adhesion point and microbial biomass have been increased, strengthened the tightness between magnetic fillers and microbial film, promote biomembranous quick formation, thereby strengthened the carrier surface forming biofilm property of magnetic fillers surface microorganism.Just can see at filling surface within 60 minutes has tangible microorganic adhesion, and by the adaptation and the process of growth of certain hour, biofilm is successful again.
Embodiment 1: take by weighing base material polyethylene particle 1800 grams, and magnetic 200 grams, coupling lubricant 20 grams are put into the injection moulding machine injection moulding after stirring, make about 35 of filler.Magnetize in the magnetic field of again filler being put into 10000 Gausses, prepared magnetic fillers has 60~120 Gausses' permanent-magnetic field.With a plurality of prepared magnetic fillers with contain magnetic mud and be placed on earlier in the sewage aerobic biological treatment device, the initial CODcr of synthetic wastewater is 410mgl -1, air flow quantity is 2lmin -1Experimental result shows, in 60 minutes, promptly there is significantly microorganic adhesion on the magnetic fillers surface, and after 4~5 days, microorganism adapts to the filling surface ambient growth, forms microbial film, the common polythene filler of finishing biofilm time ratio same structure shorten near half.
Embodiment 2: take by weighing base material polyethylene particle 3200 grams, and magnetic 500 grams, coupling lubricant 45 grams are put into the injection moulding machine injection moulding after stirring, make about 70 of filler.The magnet charger of again filler being put into 10000 Gausses magnetizes, and prepared magnetic fillers has 80~120 Gausses' permanent-magnetic field.Contrast biofilm experimental result shows that in 30~40 minutes, promptly there is microorganic adhesion on the magnetic fillers surface, the microorganism that common fillers is not then adhered to basically.After growth in 3~4 days, the unit biofilm biomass improves about 30% than common polythene filler on the magnetic fillers surface.
Embodiment 3: take by weighing base material polypropylene GRANULES 2000 grams, and magnetic 150 grams, coupling lubricant 25 grams take by weighing hydroaropic substance polyvinyl alcohol 10 grams, gac 5 grams again, put into the injection moulding machine injection moulding after stirring, and make about 45 of filler.The magnet charger of again filler being put into 10000 Gausses magnetizes, and prepared magnetic fillers has 50~100 Gausses' permanent-magnetic field.Contrast biofilm experimental result shows, at the initial CODcr 500mgl of synthetic wastewater -1, air flow quantity is 2lmin -1Under the condition, in 60 minutes, promptly there is obviously microorganic adhesion on the magnetic fillers surface, after 4~5 days, the biofilm success of magnetic fillers surface, this moment, system CODcr clearance reached 90%, was 78% and adopt the CODcr clearance of common polythene filler and traditional colonization method system, adopted novel method can improve COD of sewage cr clearance more than 10%.

Claims (3)

1, a kind of microorganism colonization method comprises the steps:
(1) filler that will have magnetic is put into the biologic packing material water treatment device;
(2) adding ferromagnetic oxide powder or strong magnetic particle are tamed in active sludge, make the part microorganic adhesion on the magnetic surface, form activation magnetic mud;
(3) again the mud that is mixed with magnetic is joined in the biologic packing material water treatment device;
(4) bubbling air, under the magnetic attraction effect of the impact of air-flow and filler, a large amount of magnetics that carry microorganism will be attached to the magnetic fillers surface rapidly, thereby realize the microorganism colonization on magnetic fillers surface.
2, microorganism colonization method as claimed in claim 1 is characterized in that, the preparation technology of described activation magnetic mud is:
Mud with the municipal sewage treatment station is bacterial classification, adds the wastewater/sewage that needs processing in right amount, adds a small amount of nutritive substance again, as glucose or KH 2P0 4, adding an amount of magnetic simultaneously, aeration mixes;
Bubbling air, at set intervals, as 1~2 day, after stop aeration, treat that microorganism and magnetic settle down after, exclude supernatant liquid and add the new wastewater/sewage of as much, so repeatedly, at room temperature cultivate a couple of days, promptly get and activate magnetic mud.
3, microorganism colonization method as claimed in claim 1 or 2 is characterized in that, the preparation technology of described magnetic fillers is:
In polymer base material, add a certain amount of magnetic and coupling lubricant, use the injection moulding machine injection moulding;
Is that magnet charger about 8000~15000 Gausses magnetizes with the gained filler by magneticstrength, can make it have nonvolatil magnetic field, and average intensity is about 50~120 Gausses.
CNA2007101057882A 2007-05-30 2007-05-30 Microorganism colonization method Pending CN101314500A (en)

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Application Number Priority Date Filing Date Title
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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101913710A (en) * 2010-08-04 2010-12-15 中国海洋大学 Suspended packing microbial quick film forming method
CN102070244A (en) * 2010-12-02 2011-05-25 暨南大学 Magnetic carrier biofilm reactor and method thereof used for wastewater sludge reduction treatment
CN103113002A (en) * 2013-03-04 2013-05-22 广东新大禹环境工程有限公司 Method for processing organic sewage
CN103641233A (en) * 2013-11-20 2014-03-19 江苏大学 Method for preparing magnetic filler by using waste plastic bottles
CN104058497A (en) * 2014-07-01 2014-09-24 李泽 Microbiological treatment device
CN104211163A (en) * 2014-09-02 2014-12-17 中国科学院广州能源研究所 Bed material carrier particles for fluidized-bed sewage treatment system and preparation method of bed material carrier particles
CN104310571A (en) * 2014-10-11 2015-01-28 昆明理工大学 Method for purifying poisonous and harmful organic pollutants by using electromagnetic collaborative enhanced biological packed tower
CN104986898A (en) * 2015-07-28 2015-10-21 东北大学 Method and device for treating heavy metal waste water by ambient-temperature ferrite cycle treatment process
CN105858915A (en) * 2016-06-20 2016-08-17 孟涛 Water purification device
CN105923940A (en) * 2016-07-18 2016-09-07 周可可 Helical sewage treatment pipeline
CN105948418A (en) * 2016-07-18 2016-09-21 汪浩 Sewage purifying technology and use method thereof
CN106966494A (en) * 2017-04-12 2017-07-21 长安大学 The method for removing nitrate nitrogen in water removal, electrode colonization method, electrode and device

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101913710B (en) * 2010-08-04 2011-10-05 中国海洋大学 Suspended packing microbial quick film forming method
CN101913710A (en) * 2010-08-04 2010-12-15 中国海洋大学 Suspended packing microbial quick film forming method
CN102070244A (en) * 2010-12-02 2011-05-25 暨南大学 Magnetic carrier biofilm reactor and method thereof used for wastewater sludge reduction treatment
CN103113002B (en) * 2013-03-04 2014-11-19 广东新大禹环境工程有限公司 Method for processing organic sewage
CN103113002A (en) * 2013-03-04 2013-05-22 广东新大禹环境工程有限公司 Method for processing organic sewage
CN103641233A (en) * 2013-11-20 2014-03-19 江苏大学 Method for preparing magnetic filler by using waste plastic bottles
CN104058497A (en) * 2014-07-01 2014-09-24 李泽 Microbiological treatment device
CN104058497B (en) * 2014-07-01 2015-11-18 李泽 A kind of microbial treatment device
CN104211163B (en) * 2014-09-02 2016-06-08 中国科学院广州能源研究所 The bed material carrier granule of a kind of fluidized-bed Sewage treatment systems and its preparation method
CN104211163A (en) * 2014-09-02 2014-12-17 中国科学院广州能源研究所 Bed material carrier particles for fluidized-bed sewage treatment system and preparation method of bed material carrier particles
CN104310571A (en) * 2014-10-11 2015-01-28 昆明理工大学 Method for purifying poisonous and harmful organic pollutants by using electromagnetic collaborative enhanced biological packed tower
CN104310571B (en) * 2014-10-11 2016-03-30 昆明理工大学 Electromagnetism cooperative reinforcing biological packing tower purification poisonous and harmful organic pollutant method
CN104986898A (en) * 2015-07-28 2015-10-21 东北大学 Method and device for treating heavy metal waste water by ambient-temperature ferrite cycle treatment process
CN104986898B (en) * 2015-07-28 2017-06-23 东北大学 A kind of method and device of normal temperature ferrite circular treatment heavy metal containing sewage
CN105858915A (en) * 2016-06-20 2016-08-17 孟涛 Water purification device
CN105923940A (en) * 2016-07-18 2016-09-07 周可可 Helical sewage treatment pipeline
CN105948418A (en) * 2016-07-18 2016-09-21 汪浩 Sewage purifying technology and use method thereof
CN106966494A (en) * 2017-04-12 2017-07-21 长安大学 The method for removing nitrate nitrogen in water removal, electrode colonization method, electrode and device

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