CN101313955B - Anti-dermatophyte compound preparation - Google Patents
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Abstract
The invention relates to a compound preparation for resisting dermatophyte, the name of which is 'compound anti-ringworm agent', belonging to the pet clinical drug research and development field. 50g of 5 Chinese traditional medicines of scutellaria, figwort, Chuangxiong, pseudolarix and magnolia officinalis are picked up respectively and added with 1,000 to 1,500mL of water, boiled by slow fire, heated and concentrated to 250mL of compound Chinese traditional medicine stock solution, the compound Chinese traditional medicine stock solution is subject to twenty-time dilution, added with acetic acid with a concentration of 1.25mg/mL, and the compound preparation liquid for resisting dermatophyte is obtained. The pungency and safety tests of the compound anti-ringworm agent show that the compound preparation has no pungency and toxicity on skin; the medicinal effect research shows that the compound preparation can effectively inhibit surface fungi of dog; the compound anti-ringworm agent is used for the whole-body medicated bath twice a week with 30 minutes each time; a spray prepared by the compound anti-ringworm agent is used to be sprayed on affected parts twice/d with successive application of 14d; and the fungus inhibiting ratio after the application of the compound anti-ringworm agent is 99.9 percent.
Description
One, technical field
The present invention relates to a kind of anti-dermatophyte compound preparation, belong to development of house pet clinical medicine and development field.
Two, background technology
In the veterinary clinic practice, dog dermatomycosis is a kind of very common and multiple disease.The excessive dog skin that attaches to of pathomycete is invaded skin and hair or toenail, a large amount of propagation, causes the generation of skin organic disease.The course of disease of dog dermatomycosis is long, healing is difficult, easily recurrence, and can propagate mutually between person to person, people and animal animal and animal, causes serious public health problem.Its average attack rate is about 10%～40%, and sickness rate height, relapse rate are also high, receives veterinary clinic worker's concern day by day.At present, Chang Yong antifungal agent mainly contains polyenoid class, azole, propylene amine, cell wall inhibitor etc.; Though these medicines and therapies related thereto all have certain curative effect, most drug prices are more expensive, have toxic and side effects, and because of the increasing of Resistant strain, the application of this type of medicine and curative effect are restricted.
Chinese medicine is the traditional advantage resource of China.Studies show that to have nearly kind more than 300 of the active Chinese medicine of better antifungal; Chinese medicine is cheap, effect is certain.In recent decades, along with improving constantly of scientific and technological level, the development and utilization of Chinese medicine has been subjected to more the attention and concern.Utilizing Chinese medicine to carry out also having obtained many progress aspect the antifungic action research.Chinese medicine is used for mycotic control, and not only effective, few side effects also are difficult for producing drug resistance.How to make full use of this advantage, develop novel antifungal drug, not only help to bring into play the advantage of China's traditional medicine and resource, and help to satisfy the quick demand of house pet market clinical medicine, have important use and be worth.Regrettably, form certain, reliable Chinese medicine antifungal preparation so far as yet.
At the dog dermatomycosis encountered pathogenic, filter out a kind of compound recipe Chinese and Western in conjunction with preparation with clinical prevention effect, be a very numerous and diverse job.At first to investigate, to separate dog dermatomycosis former, carries out the preparation and the screening of Chinese medicine, next carries out prescription and antifungal experiment, carries out the zest and the toxicity test of medicine once more, carries out pharmacodynamic experiment at last.In the hope of developing a kind of efficacy stability, reliable, economic, practical, effective compound recipe antifungal preparation is for the development and the exploitation of house pet clinical medicine are made contributions.
Three summary of the invention
Technical problem:
The objective of the invention is at the reality that still lacks the antifungal Chinese medicine compound on the present veterinary clinic, develop a kind of compound recipe Chinese and Western in conjunction with antifungal preparation.Compound recipe avirulence and the skin irritation of obtaining presses down antifungal efficient height, and bacteriostasis rate reaches more than 80% after the medication.
Technical scheme:
Anti-dermatophyte compound preparation, called after " the anti-tinea agent of compound recipe " is characterized in that, makes by the following method:
(1) adopt the Chinese medicine water extraction method to press following flow preparation Chinese medicine stock solution:
Get Radix Scutellariae, Radix Scrophulariae, Rhizoma Chuanxiong, Cortex Pseudolaricis, each 50g of Cortex Magnoliae Officinalis, add water 1000～1500mL, big fire is boiled, slow fire boils 30min, and medicine juice filters with 4 layers of cleaning, sterile gauze, keeps filtrate; Waste after the filtering residue that obtains repeats to decoct 2 times again; Merge 3 times filtrate, be concentrated into not enough 250mL, add water and be settled to 250mL and be herbal mixture stock solution through slow fire heating;
(2) get herbal mixture stock solution and do 20 times of dilutions, press 1.25mg/mL interpolation glacial acetic acid, the anti-dermatophyte compound preparation medicinal liquid.
The anti-dermatophyte compound preparation usage: its spray directly evenly sprays affected part, 2 times/d, logotype 14d; During dipping, 2 times/week, 30min/ time; Spraying and dipping are used separately, or are used.
Beneficial effect:
This achievement is developed compound recipe Chinese and Western in conjunction with the anti-tinea agent of antifungal preparation-compound recipe at the reality that still lacks the antifungal Chinese medicine compound on the present veterinary clinic, and a kind of effective dog dermatomycosis clinical prevention medicine is provided.Its major advantage and beneficial effect are as follows:
1, medicament sources is abundant: 5 flavor Chinese medicines and glacial acetic acid all belong to common drug in the compound recipe.The source is abundant, low price, and production cost is relatively low, is suitable for clinical practice widely.
2, drug effect is remarkable: experimentation shows that the anti-tinea agent of compound recipe all has 100% fungistatic effect to 4 kinds of pathogenic fungi, and medicine can play ideal bacteriostasis when low concentration.After the clinical application, the bacteriostasis rate of dog body surface fungus is reached 90.9%.
3, avirulence, no skin irritation: the anti-tinea agent of compound recipe both can be used for spray, also can be used for dipping.The safety and the irritant experiment of compound recipe show that this compound recipe safety non-toxic is to the skin nonirritant.
4, prescription is scientific and reasonable: theory of Chinese medical science thinks that mostly the mycotic cause of disease is the damp and hot steaming of accumulateing, damp invasion of lower energizer, insect poison invasion and attack.Compound recipe is formed with medicines such as Radix Scutellariae, Radix Scrophulariae, Rhizoma Chuanxiong, Cortex Pseudolaricis, Cortex Magnoliae Officinalis.Radix Scutellariae has broad-spectrum antifungal and antiinflammatory action; Radix Scrophulariae has the effect of antimicrobial and toxin thereof; The Rhizoma Chuanxiong blood-activating and qi-promoting has mycostasis, and Cortex cercis chinensis energy killing parasites for relieving itching, heat clearing and damp drying have analgesic effect; Cortex Magnoliae Officinalis has the merit for the treatment of disorders in muscles and skin necrosis, the effect of anthelmintic.They are collaborative mutually, have apparent in view external antifungal effect.Be added with the 1.25mg/mL glacial acetic acid in the compound recipe, glacial acetic acid has antifungic action when using separately, 3% concentration has direct bactericidal action, can treat various superficial skin fungus infects, adding concentration during prescription is 0.125%, the bonded external fungistatic effect of Chinese medicine and western medicine shows that this side has good inhibitory effect to 4 kinds of common dermatophytess.
Four, description of drawings
Fig. 1. (1. Sabouraudites lanosus is cultivated for separation of fungus and evaluation; 2. Sabouraudites lanosus microscopy; 3. microsporon gypseum is cultivated 4. microsporon gypseum microscopies; 5. acrothesium floccosum is cultivated; 6. acrothesium floccosum microscopy; 7. chlosma is cultivated; 8. chlosma microscopy)
Fig. 2. (1. part Chinese medicine antifungal MIC measures for Chinese medicine antifungal and skin irritation test; 2. part single medicinal material screening test; 3. damaged skin shows slight erythema; 4. the intact skin Non Apparent Abnormality changes)
Five, the specific embodiment
(1) evaluation methodology of anti-dermatophyte compound preparation and index
1, the external mycostasis of 31 flavor Chinese medicines
1) Chinese medicine is selected
The selection of 31 flavor Chinese medicines is main according to having the active Chinese medicine of antifungal in the existing report, in conjunction with factors such as its action effect, source, prices, is used for the drug candidate as prescription.Comprising:
(1) heat and toxic materials clearing away medicine: SHENGHUANG a kind of reed mentioned in ancient books, Radix Rehmanniae Preparata, Radix Sophorae Flavescentis, Cortex Phellodendri, the Radix Pulsatillae
(2) promoting blood flow and remove blood stasis drug: Rhizoma Chuanxiong, Radix Scrophulariae, Radix Salviae Miltiorrhizae, Spica Prunellae, Radix Rhapontici
(3) parasite killing: Cortex Pseudolaricis, Rhizoma Smilacis Glabrae, Fructus Quisqualis, the Radix Paeoniae Alba, the Radix Stemonae, Semen Arecae, the Fructus Kochiae, Rhizoma Smilacis Glabrae, Fructus Cnidii, Rhizoma Polygoni Cuspidati, favus skin, Herba Artemisiae Annuae
(4) QI regulating medicine: Cortex Magnoliae Officinalis, Flos Caryophylli, Rhizoma Acori Graminei
(5) aromatic damp resolving drug: Radix agastaches
(6) astringency: Fructus Mume
(7) diuretic medicine: Caulis Akebiae
(8) diaphoretic medicine: Radix Saposhnikoviae, Rhizoma Ligustici, Folium Perillae
Respectively get 50g, add water 200～300mL respectively, boil 20min after boiling, medicine juice is leached with gauze.Repeat to decoct 2 times, merging filtrate is concentrated into 50mL again.Make decocting liquid and be used for experiment, concentration 1000mg/mL.
2) pathogenic fungi separation and Culture
Outpatient service dog dermatomycosis case is that the fungus positive or Wu Shi lamp inspection are looked into the person that sees fluorescence through the scraping blade inspection, gathers the several plates that are inoculated in of hair in affected part.The 1st stage cultivated with sabouraud culture medium (SDA) after 28 ℃ of 3～4 weeks of cultivation, did bacterium colony macroscopic examination (colony growth speed, color and luster, surface configuration etc.), and carried out the fungus evaluation by microscopy (micro); Do not identify and then enter the cultivation of the 2nd stage, use other culture medium (DTM, PDA) instead and cultivated for 3～4 weeks, do fungus according to macroscopic view, micro again and identify.
Be divided into from identifying 4 kinds of encountered pathogenic funguses: Sabouraudites lanosus (Microsporum canis Bodin, 1902), microsporon gypseum (Microsporum gypseum Bodin, 1907), acrothesium floccosum (Epidermophytonfloccosum Hartz, 1870) and chlosma (Malassezia furfur (Robin) Baillon) " above fungus sees Wei Jingchao. the fungus identification handbook. the .1979 of Shanghai science tech publishing house ".
3) the external antifungal experiment of 31 flavor Chinese medicines
(1) fungus bacterium liquid prepares routine and produces the SDA culture medium, is inoculated in SDA culture medium central authorities, 28 ℃ of activation culture 7～10d with separating the pathogenic fungi of preserving; Add the normal saline 2mL that contains 0.1% tween 20 in the good lawn surface of activation; Scrape and get lawn, place tissue grinder to grind 10min, be ground-glass appearance to suspension; Utilize blood cell counting plate, adjusting bacterial concentration is (2～6) * 10
6Cfu/mL, standby.
(2) the medicine base prepares routine and produces the SDA culture medium, divides to be filled to 132 in vitro, and every pipe 1.5mL puts the inclined-plane, cools off standby; Crude drug concentration is the Chinese medicine aqueous solution of 1000mg/mL, adds above-mentioned each 1.5ml in vitro; Every flavor Chinese medicine is made 4 test tubes, only amounts to 31 * 4=124, respectively numbering; If 4 test tubes of positive controls, adding concentration respectively is the terbinafine 1.5mL of 25mg/mL, and final concentration is 12.5mg/mL; If 4 test tubes of negative control group add normal saline 1.5mL respectively; Amount to 132 in test tube.115 ℃ of autoclaving 15min put the inclined-plane, cool off standby.
(3) inoculation is drawn bacterium liquid 5 μ l with micro sample adding appliance, is inoculated in the medicine primary surface, and coating evenly; Place 28 ℃ of 2 weeks of constant temperature culture, observe growing state.According to the fungal growth and development situation, with the effect that "+" or "-" expression Chinese medicine " has " pathogenic fungi or " nothing " inhibition is grown.
The result shows that the antifungal effect of different Chinese medicines is difference to some extent.Filter out 12 flavor Chinese medicines according to qualifications according to the traditional Chinese veterinary medicine theory, be respectively: Herba Artemisiae Annuae, Cortex Magnoliae Officinalis, Cortex Phellodendri, Herba Pogostemonis, Fructus Quisqualis, Fructus Mume, SHENGHUANG a kind of reed mentioned in ancient books, the Radix Pulsatillae, Rhizoma Chuanxiong, Radix Scrophulariae, Herba Artemisiae Annuae and Cortex Pseudolaricis.
2,12 flavor Chinese medicine minimum inhibitory concentrations (MIC) are measured
1) preparation of bacterium liquid is with the method for bacterium liquid preparation in the external antifungal experiment of 31 flavor Chinese medicines.
2) to prepare the Chinese medicine original content be 1000mg/mL to the medicine base; At a kind of pathogenic fungi, prepare 60 in test tube, other adds each 1 of positive control and negative control, amounts to 62, respectively numbering.Every in vitro adds SDA culture medium 2.25mL; 12 flavor Chinese medicines, every flavor Chinese medicine is made 5 Concentraton gradient.Get the 2mL syringe, draw medicinal liquid 0.5mL, add and contain culture medium 0.25mL in vitro, the drug level dilution is 10% (being 100mg/mL).Continue to extract normal saline to the 0.5mL scale, add and contain culture medium 0.25mL in vitro, the drug level dilution is 5% (being 50mg/ml); Prepare the medicine base of 2.5% (25mg/mL), 1.25% (12.5mg/mL), 0.625% (6.25mg/mL) successively, 115 ℃ of autoclaving 15min put the inclined-plane, cool off standby.Need do 4 groups of repetitions, with at 4 kinds of different pathogenic fungi.So need 248 in test tube altogether.
3) the inoculation micro sample adding appliance is drawn bacterium liquid 5 μ l, is inoculated in the medicine primary surface, and coating evenly; Place 28 ℃ of 2 weeks of constant temperature culture, observe growing state.
4) judgement of minimum inhibitory concentration (MIC value) is according to the fungal growth and development situation, with "-", "+", " ++ ", " +++", " ++ ++ " 5 ranks, the fungistatic effect of expression Chinese medicine.4 fungal strains were respectively cultivated about 2 weeks, judged terminal point with visual method then.Compare with the growth control group, growth suppresses fully, the minimum inhibitory concentration that the limpid pairing lowest concentration of drug of culture medium is a medicine.
The fungistatic effect of 12 flavor Chinese medicines sees Table 1.
The MIC value (MIC:mg/mL) of 12 kinds of Chinese medicine extract of table 1
The result shows when middle concentration is 10% (100mg/mL), have inhibiting Chinese medicine to comprise Cortex Phellodendri, Fructus Quisqualis, Fructus Mume, SHENGHUANG a kind of reed mentioned in ancient books, Rhizoma Chuanxiong, Radix Scrophulariae, Cortex Pseudolaricis to 4 kinds of pathogenic fungi.When middle concentration is 5% (50mg/mL), all there is inhibiting Chinese medicine that Cortex Phellodendri, Fructus Quisqualis, Fructus Mume, SHENGHUANG a kind of reed mentioned in ancient books, Rhizoma Chuanxiong, Radix Scrophulariae are arranged to 4 kinds of pathogenic fungi.When middle concentration is 2.5% (25mg/mL), all there is inhibiting Chinese medicine to comprise Fructus Quisqualis, SHENGHUANG a kind of reed mentioned in ancient books, Radix Scrophulariae to 4 kinds of pathogenic fungi.
3, Western medicine minimum inhibitory concentration (MIC) is measured
Choose the special sheet of anti-tinea (special), nizoral (ketoconazole sheet 200mg/ sheet), 3 kinds of clinical results of use of glacial acetic acid (1000mg/ml) antifungal Western medicine preferably than naphthols 100mg/ sheet.
1) preparation of bacterium liquid is with the method for bacterium liquid preparation in the external antifungal test of 31 flavor Chinese medicines.
2) medicine base preparation preparation SDA culture medium; Divide to be filled to 68 in vitro the 1.5mL/ pipe.During preparation terbinafine medicine base, get 1 of terbinafine, grind, add an amount of DMSO dissolving, replenish normal saline to 50mL, concentration is 2mg/mL stock solution.During use, (1) gets stock solution 1.5mL (containing pure medicine 3mg), adds in the 1.5mL culture medium, and final concentration is 1mg/mL; (2) get stock solution 0.5mL (containing pure medicine 1mg), add the 4.5mL normal saline, reaching concentration is 0.2mg/mL; Get this liquid 1.5mL (containing pure medicine 0.3mg), add in the 1.5mL culture medium, final concentration is 0.1mg/mL; (3) obtaining final concentration successively is 0.01mg/mL, 0.001mg/mL, the medicine base 3mL of 0.0001mg/mL.
During preparation ketoconazole medicine base, get 1 of ketoconazole, grind, add the inferior maple of an amount of diformazan (DMSO) dissolving, replenish normal saline to 100mL, concentration is 2mg/mL stock solution; Step makes concentration and is respectively 1mg/mL, 0.1mg/mL, 0.01mg/mL, 0.001mg/mL, the medicine base 3mL of 0.0001mg/mL in same 2.
During preparation glacial acetic acid medicine base, suction glacial acetic acid 0.5mL (containing pure medicine 300mg) adds normal saline 24.5mL, to 20mg/mL, is stock solution; Suction stock solution 3mL injects the 1.5mL culture medium with the 1.5mL medicinal liquid, and final concentration is 10mg/mL; The residue medicinal liquid continues to be pumped to 3mL, again the 1.5mL medicinal liquid is injected the 1.5mL culture medium, and final concentration is 5mg/mL; Make 2.5mg/mL successively; 1.25mg/mL; 0.625mg/mL each 3mL of medicine base.
3) inoculation is consistent with MIC assay method in concrete experimental working technique of cultivation and the external antifungal of single medicinal material; If positive control and negative control group.(annotate: the clinical glacial acetic acid concentration of using is 30mg/ml).
The result shows that to the minimum inhibitory concentration of 4 kinds of pathogenic fungi growths, special is 0.01mg/ml than naphthols; Ketoconazole is 0.01mg/ml; Glacial acetic acid is 2.5mg/mL.
4, Chinese and Western is in conjunction with the prescription bacteriostatic test
According to the external antifungal result of the test of single medicinal material and simple western medicine, screening of medicaments is carried out prescription.Relate to 3 factors in the prescription: Chinese medicine compound, selected Western medicine, drug level (table 2).
According to Chinese medicine bacteriostatic test result of unit and simple western medicine bacteriostatic test result, determine that 3 groups are tried herb mixture, are respectively:
Herbal mixture group 1 (theoretical prescription): Radix Scutellariae, Radix Scrophulariae, Rhizoma Chuanxiong, Cortex Pseudolaricis, Cortex Magnoliae Officinalis
Herbal mixture group 2 (best single prescription): Fructus Quisqualis, Radix Scutellariae, Radix Scrophulariae, Cortex Phellodendri
Herbal mixture group 3 (outpatient service contrast side): Cortex Pseudolaricis, the Radix Stemonae, Fructus Cnidii, the Fructus Kochiae
Table 2 Chinese medicine and western medicine is in conjunction with compound recipe factor table
Result of the test shows that in the bacteriostasis to 4 kinds of Different Kinds of Pathogens funguses, difference is not remarkable between each factor.Wherein " Chinese medicine composition " played crucial relatively effect in prescription.According to data statistic analysis, comprehensive various factors, the best compound recipe of anti-tinea agent is combined as: A
1B
2C
3, promptly theoretical prescription (Radix Scutellariae, Radix Scrophulariae, Rhizoma Chuanxiong, Cortex Pseudolaricis, Cortex Magnoliae Officinalis), Chinese medicine total concentration are 5%, acetate concentration on the rocks is 1.25mg/mL.This prescription all has 100% fungistatic effect to 4 kinds of pathogenic fungi, called after " the anti-tinea agent of compound recipe ".
5, the safety and the irritant experiment of the anti-tinea agent of compound recipe
1) safety experiment
Middle concentration is 300mg/mL, and the adjustment glacial acetic acid is 30mg/mL.
Measure mice maximum tolerated dose (MTD).Get 40 of healthy kunming mices, body weight 20～30g is divided into test group and matched group at random, and 20 every group, male and female half and half.Test group adopts the medicine (being the maximum concentration of medicine) of 300mg/mL concentration, presses 0.4mL/10g body weight gastric infusion 3 times, each 8h (day dosage is amounted in accumulation: Chinese medicine 36000mg/kg body weight, glacial acetic acid 3600mg/kg body weight) at interval; Matched group is observed 7d continuously with the physiologic saline for substitute medicine after the perfusion.Whole mices adopted the cervical vertebra dislocation method to cause death in the 7th day, cutd open the pathological change of histoorgans such as the inspection and the record heart, liver, spleen, lung, kidney.The result shows that mice spirit, appetite, activity there is no ANOMALOUS VARIATIONS compared with the control, and any pathological change does not appear in internal organs; Show that this compound recipe does not have toxic action.
2) skin irritation test
2 of rabbit are about body weight 1.5kg.With 8% sodium sulfide unhairing, the unhairing district is 15cm to 24h with rabbit spinal column both sides before administration
2, 24h checks that skin of unhairing is whether injured because of unhairing after the unhairing.Be divided into 2 groups, i.e. intact skin group, damaged skin group.The making of damaged skin is adopted the sterilization syringe needle that unhairing is sterilized and is drawn " # " word on the skin, with skin oozing of blood degree of being, and the damaged degree basically identical of left and right sides skin.(1) intact skin group.One side is smeared with normal saline 5mL, in contrast; Opposite side is smeared with the anti-tinea agent of compound recipe 5mL; And isolate with the gauze dressing that is soaked with normal saline and the anti-tinea agent of compound recipe respectively and tried the position.(2) damaged skin group.Identical with the operation of intact skin group.Remove with warm water behind the 24h and tried thing and observe, smear the identical thing that tried then, 7d continuously, and behind the last coating 1,24,48 and 72h observe depilation district dermoreaction.The result shows that the anti-tinea agent of compound recipe does not cause abnormal conditions such as animal skin erythema, edema.The stimulus intensity evaluation result shows that this compound recipe does not have skin irritation.
(2) preparation and the use of anti-dermatophyte compound preparation (called after " the anti-tinea agent of compound recipe ")
Preparation method
Get 5 each 50g of flavor crude drug, add water 1000～1500mL, big fire is boiled, slow fire boils 30min, and medicine juice filters with 4 layers of cleaning, sterile gauze, keeps filtrate; Waste after the filtering residue that obtains repeats to decoct 2 times again; Merge 3 times filtrate, be concentrated into not enough 250mL through slow fire heating, adding water, to be settled to 250mL be herbal mixture original liquid (the herbal mixture total concentration is 1000mg/mL).Get original liquid and do 20 times of dilutions, and press 1.25mg/mL interpolation glacial acetic acid, anti-dermatophyte compound preparation (the anti-tinea agent of compound recipe) medicinal liquid.
Usage and test effect
Its spray directly evenly sprays affected part, 2 times/d, logotype 14d; During dipping, 2 times/week, 30min/ time.Spraying and dipping can be used separately, also can be used.
Result of the test shows, tried before the dog medication totally 24 by galley proof this, isolate fungus 22 strains, account for sample total 91.7%; The contrast dog totally 8 by galley proof this, isolate fungus 8 strains, recall rate is 100%; Once more from sampling point epilation separation and Culture fungus, tried 24 of dogs and originally isolated fungus 2 strains altogether by galley proof after the medication, account for sample total 8.3%, bacteriostasis rate is 90.9%; The matched group bacteriostasis rate is 25%.
Technological core of the present invention is to have adopted system, reliable, effective Chinese medicine screening method, the scientific and reasonable principles of formulating prescriptions, and the external Chinese medicine sensitivity testing method of standard, effective gonimoblast shape fungus comparatively.The M38-A scheme of institute of the present invention reference is to embrace a scheme that filamentous fungi antifungal drug sensitivity tests release in 2003 at product by American National clinical experiment standard committee (NCCLS).The M38-A scheme is a standardized program of measuring filamentous fungi to different antifungal drug sensitivity, the purpose of formulating this scheme is the standardized program of wishing through identical, and the drug susceptibility result that the different experiments chamber is obtained has comparability or repeatability.
Claims (1)
1. the compound preparation that is used for the treatment of dog dermatomycosis, called after " the anti-tinea agent of compound recipe " is characterized in that said preparation is prepared from by the following method:
(1) get Radix Scutellariae, Radix Scrophulariae, Rhizoma Chuanxiong, Cortex Pseudolaricis, each 50g of Cortex Magnoliae Officinalis crude drug, add water 1000～1500mL, big fire is boiled, slow fire boils 30min, and medicine juice filters with 4 layers of cleaning, sterile gauze, keeps filtrate; Waste after the filtering residue that obtains repeats to decoct 2 times again; Merge 3 times filtrate, be concentrated into not enough 250mL, add water and be settled to 250mL and be herbal mixture stock solution through slow fire heating;
(2) get herbal mixture stock solution and do 20 times of dilutions, press 1.25mg/mL interpolation glacial acetic acid, the anti-dermatophyte compound preparation medicinal liquid.
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| 甘燕珍.冰醋酸治疗耳部真菌感染340例报告.《医学文选》.1995,第16卷(第5期),第452-453页. * |
| 雷本锐等.中药对犬真菌型皮肤病的疗效观察.《畜禽业》.2006,(第16期), * |
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