CN101309590A - Antimicrobial coating based on kimchi lactic acid bacteria - Google Patents
Antimicrobial coating based on kimchi lactic acid bacteria Download PDFInfo
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- CN101309590A CN101309590A CNA2006800423777A CN200680042377A CN101309590A CN 101309590 A CN101309590 A CN 101309590A CN A2006800423777 A CNA2006800423777 A CN A2006800423777A CN 200680042377 A CN200680042377 A CN 200680042377A CN 101309590 A CN101309590 A CN 101309590A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K11/00—Use of ingredients of unknown constitution, e.g. undefined reaction products
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K3/00—Use of inorganic substances as compounding ingredients
- C08K3/02—Elements
- C08K3/08—Metals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K2201/00—Specific properties of additives
- C08K2201/011—Nanostructured additives
Abstract
The present invention discloses an article with an antimicrobial property, and a manufacturing method thereof which gives the antimicrobial property to a surface of an article which bacteria or viruses may contact to proliferate themselves, by using the Kimchi lactic acid bacteria culture fluid with antibacterial and antivirus effects. The article needing the antimicrobial effect is provided with the antimicrobial property, by using the Kimchi lactic add bacteria culture fluid with a wide antibacterial spectrum singly or in combination with nano metal particles.
Description
Technical field
The present invention relates to have the article and the manufacture method thereof of antimicrobial property, more specifically relate to article and have Pickles (Kimchi) lactic acid bacteria culture solution of antibacterium and antivirus action, can contact the manufacture method that the own article surface of breeding in back is given antimicrobial property to bacterium or virus by use with antimicrobial property.
Background technology
Recently, along with the continuous increase to the concern of health and cleaning, people have developed the product of many improve sanitary conditions and cleannes.The electronic product field is no exception.Particularly, need the household electrical appliance of health and cleaning to comprise refrigerator, washing machine, dryer, air-conditioning, air cleaner, fan, dust catcher, electric kettle, electric cooking appliance, dish-washing machine, bowl dish dryer, micro-wave oven, blender, VTR, TV, home theater etc.
Colonize in the surface of product or the bacterium or the fungi of its assembly surface and cause as atopic dermatitis, the disease of respiratory disorder etc., the damage product design produces peculiar smell, and product appearance is faded.Therefore, need produce by prevent various bacteria and fungi contact and propagation protects the user away from disease, and keep the antimicrobial article of product appearance.
Usually, the antibacterial agent that great majority are used to make the antibacterium article all is chemosynthesis, and this needs higher cost, and causes harmful side effect.Recently, to having good antibacterial characteristic, and it is more active always human body not to be had the research of natural antibacterium material of harmful side effect.
Kimchi lactic acid bacteria produces in the fermentation of Pickles and maturation.Kimchi lactic acid bacteria has obtained confirmation by long use as the safety of natural source.Kimchi lactic acid bacteria obtains with low cost easily.In addition, known kimchi lactic acid bacteria is to have good antibacterial characteristic, and the wide natural antibacterium material of antibacterium spectrum.And the existing kimchi lactic acid bacteria culture fluid that reports can suppress the activity that avian influenza virus and other and avian influenza virus have the virus of similar mechanism.
Therefore, inventor of the present invention is by giving antimicrobial property to article surface, pays close attention to the antibacterium of kimchi lactic acid bacteria culture fluid and antivirus action and finished the present invention.
Summary of the invention
Technical problem
The object of the present invention is to provide the article that are coated with kimchi lactic acid bacteria culture fluid with antimicrobial property with antibacterium and antivirus action.
Another object of the present invention is to provide the manufacture method of the article with antimicrobial property, and the kimchi lactic acid bacteria culture fluid that this method will have antibacterium and antivirus action is coated in article surface.
Technical scheme
For realizing the purpose of the invention described above, the article that are coated with kimchi lactic acid bacteria culture fluid or kimchi lactic acid bacteria culture fluid and nano-metal particle are provided with antimicrobial property.
The manufacture method of the article with antimicrobial property also is provided, has comprised the step that kimchi lactic acid bacteria culture fluid or kimchi lactic acid bacteria culture fluid and nano-metal particle is coated in article surface.
The working of an invention scheme
To describe the present invention in detail now.
The invention provides the article that are coated with kimchi lactic acid bacteria culture fluid or kimchi lactic acid bacteria culture fluid and nano-metal particle with antimicrobial property.
Here, antimicrobial property comprises antibacterial characteristic, antifungal property and ntiviral characteristic.
Article are illustrated in bacterium in the use of these article, and virus etc. can contact the own all types of article of breeding in back and not have particular restriction.Therefore, article can be according to method well known in the art, for example, casting, extrusion modling, blow molding and thermoforming are unrestrictedly made.
According to an aspect of the present invention, article can be a kind of household electrical appliance.Household electrical appliance are represented whole household electrical appliance or its inside or external component.Exemplary household electrical appliance comprise refrigerator, washing machine, dryer, air-conditioning, air cleaner, fan, dust catcher, electric kettle, electric cooking appliance, dish-washing machine, bowl dish dryer, water purifier, micro-wave oven, blender, VTR, TV and home theater.The parts of household electrical appliance are according to the difference of the kind of household electrical appliance and difference.For example, the parts of washing machine have interior bucket, outer bucket, detergent box, the cylinder lift is given/drainage channel, filter etc., the parts of refrigerator have inside/outside wall, cold air duct, ventilation blower, door handle, vegetable box, ice maker, filter etc., the parts of air-conditioning have electrical heat exchanger, ventilation blower, air channel, filter etc.Can form raw material that whole household electrical appliance or its form any kind of of parts can use and not have a particular restriction.For example, thermoplastic resin, thermosetting resin, rubber and metal all can be used as raw material.Raw material can use with distinct methods according to its characteristic.
According to another aspect of the present invention, article can be filters.The article of any execution filtering function can use, and are using, and kind and type aspect do not have particular restriction.Exemplary article comprise air cleaner, water filter and dust remover filter.Material with any kind of of filtering function all can be used as the material of filter, and to kind, type, size and manufacture method do not have particular restriction.Exemplary materials comprises glass fibre, ion-exchange fibre, and cellulose fiber peacekeeping asbestos fibre, various organic and inorfils, metal, as zinc, copper and aluminium, and plastics.These materials can use according to its characteristic conversion.The type of filter can suitably change according to the device that uses filter does not have particular restriction, as honeycomb type, and granular pattern (graintype), net type (net type) filters paper mold, cotton type, mesh type (mesh type), template and foam type.According to the present invention, this filter can be separately or with identical product in already present combination of filters use.
According to the present invention, article can be air cleaners.The article of any execution air filtering function all can be used as air cleaner, and to kind, type, size and manufacture method do not have particular restriction.And the type of air cleaner can suitably change according to the device that uses filter does not have particular restriction.According to the present invention, air cleaner can replace odor removal filter, as be used for various household electrical appliance, as refrigerator, the active carbon filter of air-conditioning and air cleaner, aluminium web filter, carbon filter and HEPA filter, and the filter of the air purifier of vehicle, perhaps can use with already present combination of filters.
According to the present invention, the kimchi lactic acid bacteria culture fluid that obtains by all means can use and not have particular restriction, as long as it has antibacterium and antivirus action.For example, the kimchi lactic acid bacteria culture fluid can directly extract from Pickles, extracts from the kimchi lactic acid bacteria of cultivating or buys on market.Any known method may be used to cultivate and extracts kimchi lactic acid bacteria and do not have particular restriction.
In addition, the kimchi lactic acid bacteria culture fluid in any stage can use and not have particular restriction, as long as it has antibacterium and antivirus action.For example, the kimchi lactic acid bacteria culture fluid can be selected from kimchi lactic acid bacteria culture fluid itself, kimchi lactic acid bacteria culture fluid concentrate, kimchi lactic acid bacteria culture fluid dry matter and composition thereof.Any known method may be used to concentrate with dry kimchi lactic acid bacteria culture fluid does not have particular restriction.
Preferred kimchi lactic acid bacteria is selected from Leuconostoc (Leuconostoc sp.) kimchi lactic acid bacteria, lactobacillus (Lactobacillus sp.) kimchi lactic acid bacteria, Wei Si Bordetella (Weissella sp.) kimchi lactic acid bacteria and composition thereof.
Preferred Leuconostoc kimchi lactic acid bacteria is selected from lemon leukonid (Leuconostoccitreum), leuconostoc lactis (Leuconostoc lactis), leuconostoc mesenteroides dextranicum (Leuconostoc mesenteroides subsp.dextranicum), leuconostoc mesenteroides mesenterium subspecies (Leuconostoc mesenteroides subsp.mesenteroides), Leuconostoc argentinum, Leuconostoc carnosum, Leuconostoc gellidum, Leuconostoc kimchii, Leuconostoc inhae, Leuconostoc gasicomitatum and composition thereof.More preferably the Leuconostoc kimchi lactic acid bacteria is selected from the lemon leukonid, Leuconostoc kimchii, leuconostoc mesenteroides and composition thereof.
Preferred lactobacillus kimchi lactic acid bacteria is selected from short and small newborn bacillus (Lactobacillus brevis), have a liking for yogurt bacillus (Lactobacillus acidophilus), bulgarian milk bacillus (Lactobacillus bulgaricus), plant breast bacillus (Lactobacillus plantarum), Lactobacillus kimchii, class plant breast bacillus (Lactobacillus paraplantarum), the crooked subspecies (Lactobacilluscurvatus subsp.curvatus) of crooked newborn bacillus, pure mellow wine breast bacillus pure mellow wine subspecies (Lactobacillus sakeisubsp.sakei) and composition thereof.
Preferred Wei Si Bordetella kimchi lactic acid bacteria is selected from Weissella koreensi, Weissellahanii, Weissella kimchii, Weissella soli, Weissella confusa and composition thereof.
According to the present invention, kimchi lactic acid bacteria can use with adhesive.Silicone-modified acrylic resin, urethane resin, acrylic resin and silicone resin can be used as adhesive, but do not wish to be limited.That is, various adhesives can use.In the non-independent use of kimchi lactic acid bacteria but under the situation that adhesive uses, adhesive plays kimchi lactic acid bacteria easily is fixed on article surface, and promotes the surface of the article that need antimicrobial property and the effect of the effect of mutually combining between the kimchi lactic acid bacteria.Therefore, adhesive has reduced the eluting rate of kimchi lactic acid bacteria, and has kept antimicrobial properties.
According to the present invention, the metallic particles with any kind of of sterilizing function can not have particular restriction as nano-metal particle.Exemplary metallic particles comprises Ag, Zn, Cu, Pt, Cd, Pd, Rh and Cr particle.Metallic particles can be used alone or as a mixture.Nano-metal particle is represented the metallic particles with the nano-grade size preparation.Can use and manufacture method is not had particular restriction with the metallic particles of any kind of of nano-grade size preparation.Nano-metal particle stops microorganism by the breeding function of restriction micro-organisms, as bacterium, and the breeding of fungi etc., and, suppress to breathe the metabolism that necessary enzyme function hinders microorganism by being impregnated into cell, thus realize sterilization.From antibacterial characteristic with to the angle of the innocuousness of environment and human body, nano-metal particle is preferably Ag, Zn and Cu nano-metal particle, more preferably nanometer Ag.Particularly nanometer Ag can be promoted the effectiveness of kimchi lactic acid bacteria culture fluid.
According to an aspect of the present invention, the kimchi lactic acid bacteria culture fluid can be coated on article surface separately, to be used to provide antimicrobial property.According to another aspect of the present invention, kimchi lactic acid bacteria culture fluid and nano-metal particle can be coated on article surface, to be used to provide antimicrobial property.Desired is to have the kimchi lactic acid bacteria culture fluid of antibacterium and antivirus action and have the synergy of the nano-metal particle of antibacterium function.According to the characteristic of article, the feature of manufacture method and the necessity of antimicrobial property, the kimchi lactic acid bacteria culture fluid can be used in combination separately or with nano-metal particle.The preferred kimchi lactic acid bacteria culture fluid of 5-20% by weight and the nano-metal particle of 100-2000ppm are applied to article surface.In view of the antibacterium and the antivirus action that surpass 99%, apply feature and composite character, this scope to the surperficial semipermanent of article give antimicrobial property.Yet, can suitably adjust when needing.
The present invention also provides the manufacture method of the article with antimicrobial property, and it comprises the step that kimchi lactic acid bacteria culture fluid or kimchi lactic acid bacteria culture fluid and nano-metal particle is coated in article surface.
With the surface-coated of article with the step of kimchi lactic acid bacteria culture fluid or kimchi lactic acid bacteria culture fluid and nano-metal particle can be according to the present invention under the universal method in field finish.Flatly the method for any kind of on coated article surface can be used.
When the kimchi lactic acid bacteria culture fluid applied separately, the kimchi lactic acid bacteria culture fluid can directly be coated on article surface.Suitable technique for fixing can be used for the kimchi lactic acid bacteria culture fluid is fixed on article surface with chemical mode or mechanical system.In addition, preparation contains the coating liquid of kimchi lactic acid bacteria culture fluid, and this coating liquid is sprayed to article surface, or article be impregnated in method in the coating liquid can be used for the surface of coated article.Coating liquid can be water or ethanol, but does not wish to be limited to this.Anyly contain an amount of kimchi lactic acid bacteria culture fluid, and can not have the particular restriction of kind and manufacture method aspect as coating liquid by applying solution from antimicrobial property to article surface that give.Known spray method can use as air-atomizing, but does not wish to be limited.The method of any kind of on coated article surface can be used equably.In addition, general dipping method can use and not have particular restriction.The amount of preferred kimchi lactic acid bacteria culture fluid but does not wish to be limited by weight in the scope of 5-20%.When needing, the amount of kimchi lactic acid bacteria culture fluid can suitably be adjusted.
Perhaps, apply together under the situation of use at kimchi lactic acid bacteria culture fluid and nano-metal particle, kimchi lactic acid bacteria culture fluid and nano-metal particle can be coated on the surface of target item successively.According to continuous coating process, nano-metal particle is coated on article surface, and the kimchi lactic acid bacteria culture fluid is applied to the surface of the article that scribble nano-metal particle then.The step that nano-metal particle is coated on article surface can be finished according to approach well known.When needing, nano-metal particle can be improved according to the characteristic of article, to be easy to coating.In addition, the step that the kimchi lactic acid bacteria culture fluid is coated on the article surface that scribbles nano-metal particle can be finished according to this area common method and not have particular restriction.Can suitably kimchi lactic acid bacteria be fixed on the article that scribble nano-metal particle with chemistry or mechanical means.The method on any coated article equably surface may be used to apply as spraying and dipping.On the contrary, also can apply the kimchi lactic acid bacteria culture fluid earlier, scribble the article surface coated with nano metallic particles of kimchi lactic acid bacteria culture fluid then.Can also be by kimchi lactic acid bacteria culture fluid and nano-metal particle be mixed, preparation contains the coating liquid of these two kinds of materials, by spraying or dipping coating liquid is coated on article surface then.Any method for preparing coating liquid can be used and not have particular restriction, as long as kimchi lactic acid bacteria culture fluid and nano-metal particle can be coated on article surface so that antimicrobial property to be provided.In the scope of 5-20%, the content of nano-metal particle is in the scope of 100-2000ppm by weight for the amount of preferred kimchi lactic acid bacteria culture fluid.In view of the antibacterium and the antivirus action that surpass 99%, apply feature and composite character, this scope to the article surface semipermanent given antimicrobial property.Yet when needing, this scope can suitably be adjusted.
In addition, according to the present invention, be coated at the kimchi lactic acid bacteria culture fluid under the situation of article surface, the kimchi lactic acid bacteria culture fluid can mix with adhesive before coating step.Adhesive is promoted the effect of mutually combining between article surface and the kimchi lactic acid bacteria culture fluid, and reduces the eluting rate of kimchi lactic acid bacteria culture fluid, thereby has kept antimicrobial properties.Therefore, use the kimchi lactic acid bacteria culture fluid more favourable separately kimchi lactic acid bacteria culture fluid and adhesive mixing ratio.Any method that the kimchi lactic acid bacteria culture fluid is mixed with adhesive can be used and not have particular restriction, as long as the kimchi lactic acid bacteria culture fluid can be coated on article surface.The content of kimchi lactic acid bacteria culture fluid and adhesive is than no particular restriction, but suitably adjusts.Can add inorganic pigment when needing.
According to one embodiment of the invention, preparation contains the coating liquid of kimchi lactic acid bacteria culture fluid and nano-metal particle, and with its by spray application on the aluminium web filter, thereby obtained to have the filter of antimicrobial property.In an experiment of the present invention, bacillus coli (Escherichia coli), staphylococcus aureus (Staphyllococcus aureus) and pseudomonas aeruginosa (Pseudomonas aeruginosa) are used to test the antibacterial activity of the filter with antimicrobial property.As a result, the filter with antimicrobial property demonstrates Escherichia coli, the good antibacterial activity of staphylococcus aureus and pseudomonas aeruginosa.In another experiment of the present invention, avian influenza virus H 5 N 1 is used to the antiviral activity of testing filters.As a result, filter demonstrates high viral slip.
On the other hand, manufacture method can also comprise after the step and washing with suitable slurry washing target item, and the kimchi lactic acid bacteria culture fluid is coated on before the article surface step by the thermal treatment dry goods.In addition, manufacture method can also comprise the kimchi lactic acid bacteria culture fluid is coated on natural seasoning after the article surface or the step by the thermal treatment dry goods.The effect that kimchi lactic acid bacteria culture fluid and nano-metal particle are fixed in article surface is played in thermal treatment.The drying time of article and baking temperature can be according to the shapes of article, and kind and size are regulated.At target item is under the situation about being made of metal, and it is favourable removing the oiliness composition that sticks to article surface in manufacturing or maintenance process.
According to the present invention, when needing, prepared article can post processing become needed shape and suitably use.Particularly, air cleaner can cut into needed size, and is used as the filter of air purifier.Air cleaner can be separately or with identical product in already present air cleaner and odor removal filter be used in combination.Air cleaner can be applied to various family expenses or commercial air purifier, refrigerator, vehicle and other household electrical appliance.
To make an explanation to the present invention by following examples now.These embodiment do not wish to be restricted.
Embodiment 1
The aluminium net that Airphil company is produced immerses about 3min in 2.5% the NaOH solution, to remove the oiliness composition.Then, the aluminium net of oil removing with 2.5% NaOH solution washing.Washing step repeats 7 times.By dry 2h in 40 ℃ drying box, washed aluminium net is heat-treated.
Embodiment 2
10g is selected from culture fluid dry powder and the silicone-modified acrylic resin adhesive of 15g of the lemon leukonid of Leuconostoc kimchi lactic acid bacteria, 3g nanometer zinc, 1g Nano Silver and 0.5g nanometer copper mix, use water as dissolution with solvents, thereby prepare the coating liquid that contains the kimchi lactic acid bacteria culture fluid.Coating liquid air-atomizing prepared aluminium to the embodiment 1 is online, the aluminium net drying that will apply then.Obtain being coated with the aluminium web filter sample of kimchi lactic acid bacteria culture fluid and nano-metal particle.
Test implementation example 1: antibacterial characteristic test
According to fask oscillating method (KSM 0146-2003) bacillus coli (ATCC 25922), the antibacterial characteristic of the aluminium net that is coated with the kimchi lactic acid bacteria culture fluid in staphylococcus aureus (ATCC 6538) and pseudomonas aeruginosa (ATCC 27853) the test implementation example 2.
Respectively with 1ml bacillus coli culture, staphylococcus aureus culture and pseudomonas aeruginosa culture are coated in aluminium web filter sample prepared among the embodiment 2 (on the 1.0cm * 1.0cm).The aluminium web filter sample immersion that is coated with each strain culture is contained in the conical flask of LB meat soup, hatch 3h at 35 ± 1 ℃ of velocity fluctuations with 120rpm.For relatively, each strain culture of 1ml is inoculated in the conical flask that contains LB meat soup, and vibration is hatched under the same conditions.The cell culture that taking-up is hatched is coated on the LB flat board, hatches 48h at 37 ℃.To each bacterial colony count.The results are shown in following table 1-3.
Table 1
| Bacterial strain | Sample | Initial inoculation amount (cfu/ml) | 1h (cfu/ml) | 2h (cfu/ml) | 3h (cfu/ml) | Inhibiting rate (%) |
| Bacillus coli | Embodiment 2 | 1.5×10 5 | ＜10 | ＜10 | ＜10 | ＞99.9 |
| Contrast | 1.5×10 5 | 1.6×10 5 | 1.7×10 5 | 2.0×10 5 |
* cfu/ml: colony-forming units/ml
Table 2
| Bacterial strain | Sample | Initial inoculation amount (cfu/ml) | 1h (cfu/ml) | 2h (cfu/ml) | 3h (cfu/ml) | Inhibiting rate (%) |
| Staphylococcus aureus | Embodiment 2 | 1.3×10 5 | ＜10 | ＜10 | ＜10 | ＞99.9 |
| Contrast | 1.3×10 5 | 1.5×10 5 | 1.8×10 5 | 2.2×10 5 |
* cfu/ml: colony-forming units/ml
Table 3
| Bacterial strain | Sample | Initial inoculation amount (cfu/ml) | 1h (cfu/ml) | 2h (cfu/ml) | 3h (cfu/ml) | Inhibiting rate (%) |
| Pseudomonas aeruginosa | Embodiment 2 | 1.2×10 5 | ＜10 | ＜10 | ＜10 | ＞99.9 |
| Contrast | 1.2×10 5 | 1.4×10 5 | 1.7×10 5 | 2.2×10 5 |
* cfu/ml: colony-forming units/ml
By last table 1-3 as can be known, compared with the control, the aluminium web filter that is coated with the kimchi lactic acid bacteria culture fluid is to microorganism, and as bacillus coli, staphylococcus aureus and pseudomonas aeruginosa have good antibacterial activity.
Test implementation example 2: ntiviral characteristic test
Preparation contains the coating liquid of kimchi lactic acid bacteria culture fluid, and is sprayed to the aluminium web filter respectively, on the surface of carbon filter and HEPA filter, thereby obtains 3 kinds of filter sample that are coated with the kimchi lactic acid bacteria culture fluid.On each filter, carry out the ntiviral characteristic test.
Antivirus action with avian influenza virus H 5 N 1 separator test kimchi lactic acid bacteria culture fluid.Use Madin-Darby canine kidney (MDCK) cell-line as the host cell of virus from MDCK.
At first, inoculation 100 μ l mdck cells (5 * 10 in every hole of 96 orifice plates
4Individual cell/ml), at 37 ℃, 5%CO
2Incubator in hatch 24h so that cell is with the bottom of individual layer shape coverage hole.In the hole of containing 3 kinds of samples (being respectively the aluminium web filter, carbon filter and HEPA filter) that are coated with the kimchi lactic acid bacteria culture fluid respectively each add viral solution with the PBS dilution (1/10, v/v), hatch at 37 ℃.In order to contrast, viral solution is hatched in the same manner with same 3 kinds of samples of uncoated kimchi lactic acid bacteria culture fluid.Add before the viral solution, write down the weight of each sample.The culture of preparation virus inoculation solution and the culture of virus inoculation solution not are respectively as the positive control and the negative control of virocyte pathology effect (vCPE).
For the test antivirus action, will be coated with the aluminium filter sample of kimchi lactic acid bacteria culture fluid, the culture of the aluminium filter sample of uncoated kimchi lactic acid bacteria culture fluid and 10 times of dilutions of positive control is seeded among 7 rows of 96 orifice plates, 4 parts in every sample.The culture of negative control is inoculated in the 8th last row.After the inoculation, dull and stereotyped at 5%CO
2Incubator in hatched 3 days in 37 ℃.Observe dull and stereotyped vCPE, measure virus titer TCID
50(50% TCID).Antivirus action is represented with viral slip (%).Herein, viral slip is to transform the logTCID that obtains with the resulting value of weight that the weight of sample that is coated with the kimchi lactic acid bacteria culture fluid deducts the sample of uncoated kimchi lactic acid bacteria culture fluid
50The percent value of/ml.The results are shown in following table 4-6.
Table 4
| Virus titer (TCID 50/ml) | The Al web filter (g) that has applied | Uncoated Al web filter (g) | Virus slip (%) | |
| 30min | 6.25 | 0.1132 | 0.0946 | 99.92 |
| 1h | 6.50 | 0.1091 | 0.0871 | ＞99.99 |
| 2h | 6.27 | 0.0999 | 0.0809 | ＞99.99 |
| 4h | 6.25 | 0.1195 | 0.0872 | ＞99.99 |
| 8h | 5.75 | 0.1175 | 0.0806 | 99.99 |
Table 5
| Virus titer (TCID 50/ml) | The C filter (g) that has applied | Uncoated C filter (g) | Virus slip (%) | |
| 30min | 6.75 | 0.3194 | 0.2720 | 99.90 |
| 1h | 6.50 | 0.3240 | 0.2743 | 99.84 |
| 2h | 6.00 | 0.3196 | 0.2635 | ＞99.99 |
| 4h | 5.75 | 0.4346 | 0.2887 | 99.82 |
Table 6
| Virus titer (TCID 50/ml) | The HEPA filter (g) that has applied | Uncoated HEPA filter (g) | Virus slip (%) | |
| 30min | 6.00 | 0.0820 | 0.0614 | 98.22 |
| 1h | 5.75 | 0.0848 | 0.0678 | 99.82 |
| 2h | 6.50 | 0.0545 | 0.0514 | 99.94 |
| 4h | 6.25 | 0.0560 | 0.0486 | 99.99 |
| 8h | 5.75 | 0.0529 | 0.0461 | 99.99 |
By last table 4-6 as can be known, be coated with the aluminium web filter of kimchi lactic acid bacteria culture fluid, carbon filter and HEPA filter all have almost the viral slip greater than 99%, that is, and and good antivirus action.
Application Example
According to the present invention, described article can be used for various uses.
Need the household electrical appliance of antimicrobial property that refrigerator is arranged, washing machine, air-conditioning, dust catcher, air cleaner, water purifier, humidifier, dish-washing machine, micro-wave oven, VTR, computer, TV etc.
1) is applied to refrigerator
The present invention can be applied to the inwall of refrigerator, cold air duct, ventilation blower, door handle, vegetable box, air cleaner, ice maker, basket and outer surface.
2) be applied to washing machine
The parts of washing machine, as interior bucket, outer bucket, electromagnetic shaker, detergent box, the surface of cylinder lift and bucket can apply.The present invention can be applied to dry channel, gives/filter (air cleaner and water filter) of drainage channel and circulation canal.
3) be applied to air-conditioning
The present invention can be applied to the fin of heat exchanger, electrical heat exchanger (ventilation components), ventilation blower (metal and molding substance), the air cleaner of air channel (getter device, exhaust apparatus, shutter, grid etc.) and indoor set.
4) be applied to air cleaner
Special kimchi lactic acid bacteria culture fluid filter can be installed, as one of multi-filter of air cleaner.
5) be applied to dust catcher
The user is usually with his/her hand contact dust catcher.Therefore, can apply the surface of dust catcher.The inboard that applies dust catcher can strengthen bactericidal action.The present invention can also be applied to the discharge filter of dust catcher.
6) be applied to dish-washing machine
The present invention can be applied to bucket, sewage pool, filter screen, spray arm, water filter etc.
Although described the preferred embodiments of the invention, will be appreciated that the present invention should not be subject to these preferred embodiments, those skilled in the art can carry out various changes and modification in the present invention's spirit and scope required for protection.
Industrial usability
As previously mentioned, according to the present invention, by being used in combination the wide kimchi lactic acid bacteria nutrient solution of antibacterium spectrum separately or with nano-metal particle, need the article of anti-microbial effect to be endowed antimicrobial property. Because the good antiviral activity of kimchi lactic acid bacteria nutrient solution, article also have been endowed the ntiviral characteristic that avian influenza virus and other and avian influenza virus are had the virus of similar mechanism.
Claims (20)
1. the article that have antimicrobial property, it is coated with kimchi lactic acid bacteria culture fluid or kimchi lactic acid bacteria culture fluid and nano-metal particle.
2. the article with antimicrobial property of claim 1, wherein the kimchi lactic acid bacteria culture fluid is selected from kimchi lactic acid bacteria culture fluid itself, kimchi lactic acid bacteria culture fluid concentrate, kimchi lactic acid bacteria culture fluid dry matter and composition thereof.
3. claim 1 or 2 article with antimicrobial property, wherein kimchi lactic acid bacteria is selected from Leuconostoc (Leuconostoc sp.) kimchi lactic acid bacteria, lactobacillus (Lactobacillus sp.) kimchi lactic acid bacteria, Wei Si Bordetella (Weissella sp.) kimchi lactic acid bacteria and composition thereof.
4. the article with antimicrobial property of claim 3, wherein the Leuconostoc kimchi lactic acid bacteria is selected from lemon leukonid (Leuconostoc citreum), leuconostoc lactis (Leuconostoc lactis), leuconostoc mesenteroides dextranicum (Leuconostocmesenteroides subsp.dextranicum), leuconostoc mesenteroides mesenterium subspecies (Leuconostoc mesenteroides subsp.mesenteroides), Leuconostocargentinum, Leuconostoc carnosum, Leuconostoc gellidum, Leuconostockimchii, Leuconostoc inhae, Leuconostoc gasicomitatum and composition thereof.
5. the article with antimicrobial property of claim 3, wherein the lactobacillus kimchi lactic acid bacteria is selected from short and small newborn bacillus (Lactobacillus brevis), have a liking for yogurt bacillus (Lactobacillus acidophilus), bulgarian milk bacillus (Lactobacillusbulgaricus), plant breast bacillus (Lactobacillus plantarum), Lactobacilluskimchii, class plant breast bacillus (Lactobacillus paraplantarum), the crooked subspecies (Lactobacillus curvatus subsp.curvatus) of crooked newborn bacillus, pure mellow wine breast bacillus pure mellow wine subspecies (Lactobacillus sakei subsp.sakei) and composition thereof.
6. the article with antimicrobial property of claim 3, wherein Wei Si Bordetella kimchi lactic acid bacteria is selected from Weissella koreensi, Weissella hanii, Weissella kimchii, Weissella soli, Weissella confusa and composition thereof.
7. the article with antimicrobial property of claim 1, wherein nano-metal particle is selected from Ag, Zn, Cu, Pt, Cd, Pd, Rh, Cr and composition thereof.
8. the article with antimicrobial property of claim 7, wherein nano-metal particle is selected from Ag, Zn, Cu and composition thereof.
9. the article with antimicrobial property of claim 1, it is a kind of whole household electrical appliance or household electrical appliance, as refrigerator, washing machine, dryer, air-conditioning, air cleaner, fan, dust catcher, electric kettle, electric cooking appliance, dish-washing machine, bowl dish dryer, water purifier, micro-wave oven, blender, VTR, the inside of TV and home theater or external component.
10. the article with antimicrobial property of claim 1, it is a filter.
11. the article with antimicrobial property of claim 10, the material of its middle filtrator is selected from glass fibre, ion-exchange fibre, cellulose fiber peacekeeping asbestos fibre.
12. the article with antimicrobial property of claim 10, the material of its middle filtrator are metal or plastics.
13. the article with antimicrobial property of claim 10, the type of its middle filtrator is selected from honeycomb type, granular pattern, and the net type filters paper mold, cotton type, mesh type, template or foam type.
14. the article with antimicrobial property of claim 10, its middle filtrator is an air cleaning filter.
15. the article with antimicrobial property of claim 1 also comprise being selected from silicone-modified acrylic resin urethane resin, the adhesive of acrylic resin and silicone resin.
16. the article with antimicrobial property of claim 1, wherein the kimchi lactic acid bacteria culture fluid is with the amount coating of 5-20% by weight, and nano-metal particle applies with the amount of 100-2000ppm.
17. the manufacture method of the article with antimicrobial property described in the claim 1 is included in the step that article surface applies kimchi lactic acid bacteria culture fluid or kimchi lactic acid bacteria culture fluid and nano-metal particle.
18. the manufacture method of claim 17, wherein coating step comprises that the coating liquid that will contain kimchi lactic acid bacteria culture fluid or kimchi lactic acid bacteria culture fluid and nano-metal particle is sprayed to article surface, or article are immersed in step in the coating liquid.
19. the manufacture method of claim 17, wherein coating step comprises nano-metal particle is coated on article surface, then the kimchi lactic acid bacteria culture fluid is coated on the consecutive steps of the article surface that scribbles nano-metal particle.
20. each preparation method of claim 17-19, to be selected from silicone-modified acrylic resin before also being included in coating step, urethane resin, the step that the adhesive of acrylic resin and silicone resin mixes with the kimchi lactic acid bacteria culture fluid.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2005-0109352 | 2005-11-15 | ||
| KR10-2005-0109351 | 2005-11-15 | ||
| KR1020050109352A KR20070051587A (en) | 2005-11-15 | 2005-11-15 | Antimicrobial stuff comprising kimchi lactic acid bacteria fermented-solution capsule and manufacturing method thereof |
| KR10-2005-0112626 | 2005-11-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101309590A true CN101309590A (en) | 2008-11-19 |
Family
ID=38274710
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2006800423777A Pending CN101309590A (en) | 2005-11-15 | 2006-11-15 | Antimicrobial coating based on kimchi lactic acid bacteria |
Country Status (2)
| Country | Link |
|---|---|
| KR (1) | KR20070051587A (en) |
| CN (1) | CN101309590A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102574045A (en) * | 2009-11-03 | 2012-07-11 | Lg电子株式会社 | Air cleaning filter comprising kimchi lactic acid bacteria and disinfectant and process for producing the same |
| CN103459338A (en) * | 2011-03-28 | 2013-12-18 | 康宁股份有限公司 | Antimicrobial action of copper in glass |
| CN103596579A (en) * | 2011-05-12 | 2014-02-19 | 普罗碧欧尼株式会社 | Rice-fermented food composition containing a rice-sweetened liquid, fermented by means of kimchi lactobacillus, as an effective ingredient, and having antibacterial and antiviral effects |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0618801A2 (en) | 2005-11-23 | 2011-09-13 | Lg Electronics Inc | refrigerator |
| CN102687857B (en) * | 2012-05-24 | 2013-08-07 | 黑龙江大学 | Preparation method of lactobacillus-carrying soybean protein isolate/pectin/chitosan composite microcapsules |
-
2005
- 2005-11-15 KR KR1020050109352A patent/KR20070051587A/en not_active Application Discontinuation
-
2006
- 2006-11-15 CN CNA2006800423777A patent/CN101309590A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102574045A (en) * | 2009-11-03 | 2012-07-11 | Lg电子株式会社 | Air cleaning filter comprising kimchi lactic acid bacteria and disinfectant and process for producing the same |
| CN102574045B (en) * | 2009-11-03 | 2015-01-21 | Lg电子株式会社 | Air cleaning filter comprising kimchi lactic acid bacteria and disinfectant and process for producing the same |
| CN103459338A (en) * | 2011-03-28 | 2013-12-18 | 康宁股份有限公司 | Antimicrobial action of copper in glass |
| CN103596579A (en) * | 2011-05-12 | 2014-02-19 | 普罗碧欧尼株式会社 | Rice-fermented food composition containing a rice-sweetened liquid, fermented by means of kimchi lactobacillus, as an effective ingredient, and having antibacterial and antiviral effects |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20070051587A (en) | 2007-05-18 |
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Application publication date: 20081119 |