CN101287847A - 涉及不对称细胞分裂的基因 - Google Patents
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Abstract
本发明涉及分离涉及不对称细胞分裂过程的基因的方法。本发明还涉及使用该方法分离的基因，和它们在控制根形成，优选侧根形成中的用途。
Description
本发明涉及分离涉及不对称细胞分裂过程中的基因。本发明还涉及使用该方法分离的基因，和它们在控制根形成，优选侧根形成中的用途。
为了产生多细胞生物中存在的多种不同的细胞类型，导致具有不同命运的子代细胞的细胞分裂在多种发育过程中是重要的和决定性的(Scheres& Benfey，1999)。该类型的分类被称作不对称的，无论不对称在分裂时是否在形态学上可见(Horvitz & Herskowitz，1992)。特别在植物中，细胞移动受到限制，通常认为细胞分裂平面的控制对于规则模式的形成，即胚胎发生或气孔形成、分生组织中有序细胞列的形成中正确的分裂是重要的(Scheres & Benfey，1999)。
在植物生命周期中，发生几种不对称分裂：(a)合子的首次分裂(Mansfield & Briarty，1991)；(b)胚胎分裂，其产生静止中心的透镜状祖细胞(Dolan等人，1993)；(c)雄性小孢子分裂(Twell等人，1998)；(d)气孔复体形成期间的分裂(Larkin等人，1997)；(e)早期胚中的定向平周分裂，其分离三种主要组织：表皮、基本组织和维管组织的祖细胞(Jurgens & Mayer，1994)；(f)干细胞分裂，其分离根中分化活性子细胞和新的干细胞(Dolan等人，1993；van den Berg等人，1995)；和(g)也在侧根起始期间(Casimiro等人，2003)。
不对称分裂与标准增殖分裂根本不同之处在于它们的发生受到时空方面。此外，涉及的细胞数目是最少的。这些特征使得难以分析在该过程中(基因组范围)转录物表达。
迄今在多种生物中关于涉及不对称细胞分裂的过程仅仅进行了很少的转录物分布实验，例如，果蝇中gliogenesis期间(Egger等人，2002)，拟南芥花粉发育(Honys & Twell，2003，2004；Becker等人，2003)和侧根起始(Himanen等人，2004)。然而，这些方法都没有针对或导致鉴定驱动不对称分裂自身的遗传途径。
对于侧根起始的情况，一些中柱鞘细胞背斜和不对称地分裂(Casero等人，1993)。这不是连续的过程并且暴露于多种环境线索和内源信号。此外，这些分裂仅仅在与木质部极密切接近的那些中柱鞘细胞列中发生(Casimiro等人，2003)。
微阵列方法已经揭示向LRI的生长素信号传递的更宽的视野(Himanen等人，2004)。对于这些分析，使用侧根诱导系统。在该系统中，在补充NPA的培养基上生长的幼苗中生长素运输、信号传递和G1-向-S细胞周期转换被阻断。随后，将这些幼苗转移到含有生长素(NAA)的培养基中1-12小时。这允许生长素信号传递的可诱导的开始和通过G向S转换进展(Himanen等人，2002)。
该侧根诱导系统的改进系统也可用于研究不对称细胞分裂。我们给出了一种独特方法，其允许我们避开像组织特异性和与在LRI期间在木质部极特异分离不对称分裂的中柱鞘细胞相关的细胞的有限数目等问题。因此，我们组合了4种策略：1)最近开发的侧根诱导系统，其在LRI期间同步诱导不对称分裂(Himanen等人，2002)，2)木质部极中柱鞘特异性GFP标记株系(marker line)(J0121)，3)荧光辅助的细胞分选方法(Birnbaum等人，2003)，和4)对分离的木质部极中柱鞘细胞的基因组范围的微阵列分析。该组合的策略允许我们不仅鉴定直接参与LRI过程的那些基因，而且将结果外推到不对称分裂的一般概念。我们发现作为不对称分裂的潜在调节子的涉及细胞周期调节的基因和与细胞骨架组织和动力学相关的高百分比的基因。
本发明的第一方面是提供分离涉及不对称细胞分裂的基因的方法，其包括：(1)将野生型植物的根进行处理，诱导以同步方式发生侧根起始；(2)将由于生长素信号传递缺陷而不产生侧根的突变体的根进行以同步方式诱导野生型中侧根起始的处理；(3)鉴定在野生型但不在突变体中被诱导的基因；(4)鉴定在侧根起始期间在野生型的木质部极中柱鞘中被诱导的基因。如这里所用的早期侧根起始指刚好在中柱鞘中第一次分离前的不同阶段的事件。优选地，这在侧根的生长素诱导后10小时内，更优选在所述诱导8小时内，甚至更优选在所述诱导后6小时。优选地，使用的突变体是slr-1突变体。
优选地，所述方法还包括使用木质部极中柱鞘标记株系，接着进行细胞分选。甚至更优选地，所述标记是GFP。最优选地，通过微阵列分析在基因组范围分析所分离的细胞。
本发明的另一方面是涉及早期侧根形成的基因，其通过本发明的方法分离。优选地，所述基因编码转录因子。甚至更优选地，所述转录因子选自SEQ ID N°1到SEQ ID N°19。
由于转录因子通常在活性组织中表达，所以它们的启动子可能具有经济用途。此类启动子可以用于几种策略中来增强植物的病原体耐受性/抗性。
本发明的另一方面是用根据本发明的方法分离的涉及不对称细胞分裂的基因。优选地，所述方法还包括使用木质部极中柱鞘标记株系，接着进行细胞分选。甚至更优选地，所述基因包含编码选自SEQ ID N° 20-SEQ IDN° 34的蛋白质的序列，或者其同源物。
本发明的再一方面是涉及早期侧根形成的转录因子，其中所述转录因子选自SEQ ID N°1到SEQ ID N° 19。优选地，用本发明的方法分离编码所述转录因子的基因。
本发明的另一方面是用本发明的方法分离的基因调节早期侧根起始的用途。如这里使用的调节可以是侧根数目的增加或减少，它可以是侧根大小的增加或减小，或者它可以是侧根形成时间的偏移(植物发育中更早或更晚)。优选地，所述调节是侧根的增加或减少，甚至更优选地，它是侧根的增加。优选地，所述基因编码转录因子。甚至更优选地，所述基因编码选自SEQ ID N°1到SEQ ID N°34的转录因子，或其同源物。所述基因可以组合使用以增加对侧根形成的效果。
这里使用的基因指基因组序列(包括可能的内含子)以及从经剪接的信使RNA衍生的cDNA。它也可以指启动子序列。然而，本领域技术人员清楚对于一些应用，编码序列，如来自cDNA的编码序列，可以优选连接合适的启动子。有效连接指并列，其中所述组分处于允许它们以它们预期的方式发挥功能的关系。“有效连接”编码序列的启动子序列以这种方式连接使得在与启动子序列相容的条件下实现编码序列的表达。
如此处所用的同源物指如通过BLASTP搜索测量的(Altschul等人，1997)，所述基因编码的蛋白质具有至少75％同一性、甚至更优选至少80％同一性、甚至更优选至少85％同一性、甚至更优选至少90％同一性、甚至更优选至少95％同一性、甚至更优选至少96％同一性、甚至更优选至少97％同一性、甚至更优选至少98％同一性、甚至更优选至少99％同一性的氨基酸序列。
附图简述
图1：侧根诱导系统(A-G)NAA处理的12小时时间系列(蓝色染色＝Arath的P::GUS报道子活性；CYCB1；1标记G2/M转换)。生长素转运抑制阻断所有侧根起始，允许通过生长素同步诱导侧根，导致转移到生长素培养基后6到8小时G2/M转换的开始(D，E)。
图2：侧根起始期间主要事件的方案。
图3：对20个聚簇范围的品质因数计算。
图4：交叉表表示，具有表达谱所有组合的频率。
图5：如与野生型相比，在10天龄幼苗中CYCD3；1、E2Fa/DPa和CDKB1；1的过表达表型。
图6：J0121xUAS：bdl(0.0±0.0)中BDL蛋白质的稳定化突变形式的木质部极中柱鞘特异性表达，导致无侧根的表型，而对照株系Col-0(3.0±0.1)、J0121(4.1±0.1)、UAS：bdl(3.1±0.1)、UAS：BDL(2.8±0.1)和J0121xUAS：BDL(3.6±0.1)不显示出侧根数目/cm的减少。
图7：CYCA2；4家族中多种成员的组合突变导致侧根密度显著减小(左图：Col-0对照；右图：多个突变体)。
图8：来自多个聚簇的几种同源SALK T-DNA插入突变体的侧根表型的分析。突变体的代码在表5中列出。
图9：在完整数据集中多种上调和下调基因的早期侧根形成期间的详细分析(不对称细胞分裂通过箭头指出)。
数字指表6中列出的融合。
实施例
实施例的材料和方法
基于WT Col-slr比较的方法
取样
在侧根诱导系统中，在生长室中垂直方向的正方形平板(GreinerLabortechnik，Frickenhausen，德国)上含有10μM N-萘基邻氨甲酰苯甲酸(NPA)的标准Murashige & Skoog培养基上在连续光照(110μE·m-2·s-1PAR，通过冷白色荧光钨管提供[Osram，München，德国])下22℃使种子(Col-0 & slr-1)萌发(Himanen等人，2002)。萌发后72小时(0h时间点)，仅仅将完全接触培养基的那些幼苗(野生型和突变体)转移到含有10μM萘乙酸(NAA)的培养基中并在2小时和6小时后收获。为野生型和突变体提供这3个时间点。通过将幼苗转移到没有加入NAA的标准Murashige &Skoog培养基，为野生型包括仅仅假处理。对于所有时间点，仅仅侧根可诱导的节段用于分析。为此目的，手工除去根顶端分生组织和下胚轴以减小其他细胞类型的污染。重复所有处理。
微阵列和聚簇
使用RNeasy Minikit(Qiagen)提取RNA。使用RNA 6000 Nano LabChip Kit(Agilent Technologies，德国)分析RNA质量和数量。5.8μg总RNA用于微阵列。用Life Technologies cDNA合成试剂盒(Synthesis Kit)合成双链cDNA。将双链cDNA转化成生物素标记的cRNA(来自Enzo(LOXO GmbH)的Ambion MEGA script T7体外转录试剂盒和含有生物素的核糖核苷酸)。15μg片段化的cRNA用于与ATH1 Affymetrix基因芯片杂交。用藻红蛋白-链霉抗生物素蛋白标记显示生物素标记的RNA。ATH1基因芯片(Affymetrix)代表22747种拟南芥基因(拟南芥基因组中～85％预测的基因)。
使用Microarray Suite 5.0软件(Affymetrix)将不同芯片的总体信号归一化。原始数据呈指数分布并且因此在进一步统计分析前进行2log-转化。通过方差分析(ANOVA)分析每个基因的统计学显著性。这导致方差的三个来源的p值：时间过程的影响、基因型的影响和它们的相互作用的影响。对于基因组范围的转录物分布，严格性增加到p＜0.001。这等价于如果进行22747个测试，那么有23个假阳性测试。在该显著性水平，标记了3110种基因。
由于我们需要检测两种基因型中表达谱之间的差异，我们需要工具来最佳显示这些差异。我们通过按照时间点为两种基因型合并时间过程数据得到该工具。随后处理该合并的数据集，就好像它是单个时间过程(重复的时间点用＊指出)(0/0＊/2/2＊/6/6＊)。在聚簇前，对一系列聚簇计算聚簇算法的预测力的估计(品质因数)。品质因数越低，聚簇的预测力将越高(Yeung等人，2001)。选自聚簇数(其最小的增量不导致品质因数的降低)作为最佳聚簇数。使用TIGR Multi-experiment Viewer 2.2(http://www.tigr.org/，10/11/2003)进行所有聚簇计算。
每个基因与两个聚簇相关，代表它在野生型和突变体中的平均表达谱。以交叉表形式表示组合潜力，指出每个组合的发生频率。应用色码作为聚簇之间差异的指示器。对于所有聚簇，将平均谱的0小时和2小时之间和0小时和6小时之间表达谱的相对诱导/减小率相互比较。如果这些相对诱导/减小率在这些比较水平之一相差两倍或以上，那么对该聚簇组合分配橙色或蓝色。如果这些相对诱导/减小率在两个水平相差两倍或以上，那么对该聚簇组合分配红色。当表达水平的诱导率对于两个时间间隔(0-2和0-6)大于2倍，那么认为聚簇被上调。仅仅聚簇1、2、3和4满足这些标准。
细胞分选方法
取样
在侧根诱导系统中，将种子(J0121，http://www.plantsci.cam.ac.uk/Haseloff/geneControl/catalogues/Jlines/reco rd/record 0.html)萌发(Himanen等人，2002)。如上所述，在2小时和6小时后收获幼苗。对于所有时间点，将根切成小的0.5mm碎块，并将这些节段根据Birnbaum等人(2003，2005)进行原生质体化。在荧光激活细胞分选仪(Becton Dickinson FACSVantage)上分离表达GFP的细胞。将细胞直接分选到裂解缓冲液(Qiagen RLT缓冲液)中，混合并在-80℃立即冷冻用于以后的RNA提取。重复所有处理。
微阵列和聚簇
然后将小样品的标准Affymetrix方案用于扩增、标记和杂交RNA样品(http://www.wi.mit.edu/CMT/protocols/AffySmlSamplProto.pdf)。如
表达分析技术手册(Expression Analysis Technical Manual)中所述的将杂交的cRNA片段化。根据Affymetric方案(http://www.wi.mit.edu/CMT/protocols/Affymetrix％20User％20Manual.p df)进行杂交、洗涤和染色步骤。
使用混合模型(Mixed Model)处理数据。进行该混合模型方差分析以鉴定不同处理之间差别表达的基因(Chu等人，2002，2004)。在该方法中，通过为每个阵列将log2转化的值的平均值以0为中心，应用全局归一化步骤减小一般的阵列水平影响(Chu等人，2002)。然后除去具有与探针组平均值相比大于两个标准差的值的异常值探针。接着，对从总体归一化值得到的经转化和中心化的强度值应用混合模型ANOVA。该基因模型是基于Chu等人(2002)开发的，可以用公式表示为：
log2(PMjkl)＝Tj+Pk+Al(j)+εjkl
其中PM变量指每个基因的总体归一化步骤的输出，如上述。符号T、P和A分别代表处理、探针和阵列效应。认为阵列效应Al(j)是正态分布的随机效应(Chu等人，2002)。对该模型应用标准误术语εjkl。此外，下标j、k和l代表对kth探针和lth平行测定的jth处理(Chu等人，2002)。该模型的输出是基于全局模型的每个基因的平均表达值，以及从该基因模型得到错误排除非差异表达的0假设的概率的p值(α＝.05)。用统计学软件SAS(版本8.2)在Linux服务器上运行全局和基因模型。
使用TIGR MeV 3.0.3(Saeed等人，2003)进行来自统计分析的1920种显著差别表达的基因分组成10个聚簇。
实施例1：采样和微阵列分析
最近，我们开发了基于生长素的侧根诱导系统(Himanen等人，2002)。基于该独特的植物内(in planta)可诱导的系统，我们进行了基因组范围的转录物序型分析，以鉴定侧根起始的关键调节子。为了方便鉴定在与侧根起始相关的生长素信号传递中具有作用的那些基因，我们包括突变体作为阴性对照。选择该突变体(solitary root(单根))主要是因为它不能形成侧根和因为受影响的基因涉及生长素信号传递的已知部分(Fukaki等人，2002)。侧根诱导系统中野生型和突变体的比较对于选择突变体(IAA14/SLR)中受影响的蛋白质下游的涉及侧根起始的基因是重要的。
以这样的方式选择时间点使得我们可以监测刚好在中柱鞘第一次分裂前的不同阶段的基因表达。Himanen等人(2002)表明在转移到含有生长素的培养基之后8到10小时发生该事件。0时间点(72h NPA)与G1/S-阻断状态相一致，而转移到生长素后6小时，与木质部极相邻的中柱鞘细胞接近开始G2/M转换。此外，生长素处理后1.5到2小时报导用DR5::GUS显示了根中最早的生长素应答(图2)。因此，包括一个时间点(2h NAA)以代表该最早的生长素调节的转录。
对野生型和突变体(slr-1)都进行这些处理。此外，为野生型包括假处理以评估由于转移引起的差别基因表达。所有处理都在生物学上重复，增加数据的统计学显著性。
实施例2：统计学分析和聚簇
归一化和转换后，将数据进行ANOVA分析。以前限制的转录物序型分析(对4600个基因)(Himanen等人，未公布的结果)与当前的比较清楚地表明我们的侧根诱导系统是高度可再现的，因为当在相同显著性水平(p＜0.005)上检查时，证实了64％的差别表达的基因。为了进一步减小假阳性的量，我们应用比以前的转录物序型分析中高5倍的严格性(p＜0.001)。在该高严格性水平仍然有3110种基因被差别调节。
野生型和单根所有数据点分别的聚簇不能满足我们评估两种基因型中表达谱中差别的需要。为了满足该标准，将两种基因型的数据在一个数据集中组合。每个基因在组合的数据集中被代表两次，导致6220个表达谱。为了估计聚簇的最佳数目，为一系列聚簇计算品质因数(FOM)。在14个聚簇处估计最小的FOM，代表对应于最高预测力的聚簇的最优数目(图3)。
随后，将所有6220个表达谱聚簇到14个聚簇。以这种方式，每个基因分配两个坐标，代表两种基因型中的表达谱。所有196(14×14)个可能的组合与每个组合中基因的绝对频率一起在图4中表示。聚簇之间的不同通过色码表示。
红色表示的基因(305)代表野生型基因表达总是高于单根中的基因。在野生型中诱导并且在单根中较少诱导的这种类型的基因最可能涉及侧根起始。因此，我们集中在以野生型中以聚簇1、2、3和4表示的基因。以这种方式，我们可以将被显著调节的基因的总数(3110)减少到266(～9％)种，它们可能在侧根起始中具有关键作用。
实施例3：过滤对于一般功能类别的影响
根据MATDB(MIPS拟南芥数据(MIPS Arabidopsis ThalianaDataBase))聚簇之前和之后属于一个功能类别的基因百分数的比较很好地阐明我们的基于交叉表的聚簇的有效性(表1)。该过滤步骤清楚地导致涉及细胞周期、RNA加工、DNA合成和信号传递和发育的基因的富集。这些特征证实使用我们的侧根诱导系统，监测中柱鞘中细胞周期的进展是可能的。此外，我们发现较高百分比的基因涉及转录调节，表明对增加的转录活性的总体需要。未分类和未知的基因的百分比保持在约相同水平。而且，通过所应用的选择标准实现了涉及胁迫、转运和代谢的基因的强烈的相对减少。此外，涉及转运的基因数目的下降也可以被分类为涉及解毒和胁迫应答的基因数目下降。
实施例4：侧根起始期间细胞周期调节
选择的详细实验揭示了G1/S和S期标记，如Arath；CYCD3；2和Arath；CYCA2；4。此外，与S-期进入/进展的联系从不遥远，因为存在涉及DNA复制和蛋白质合成的基因的多数表示。这强调了我们的方法研究生长素介导的细胞周期调节的合适性。与核心细胞周期事件一样有趣的是，它们需要上游信号级联，如生长素信号传递。
实施例5：侧根起始期间的生长素信号传递
在我们的严格选择中，检测到一些基因属于在生长素信号传递中具有已知作用的基因家族，如Aux/IAA、ARF、ATGH3和ATSAUR(Hagen &Guilfoyle，2000)。属于Aux/IAA基因家族基因中的不同突变具有侧根表型(Fukaki等人，2002；Park等人，2002)。它们的基因产物抑制ARF转录因子二聚体的活性(Leyser，2002)。
最近，研究人员通过分析活性标记的株系研究了ATGH3基因产物的功能(Takase等人，2004)。这些GH3蛋白质的一些已经显示出腺苷酸化植物激素并且基于它们的底物特异性和蛋白质结构，它们被细分成三个主要类别(Staswick等人，2002)。组I的成员，如ATGH3-1、ATGH3-5和ATGH3-6/DFL1可以腺苷酸化IAA，负调节生长素活性(Takase等人，2004)。
对于小生长素Up RNAs(ATSAUR)，关于它们在生长素应答中的功能知之甚少。然而，几年前就已经报导了它们的生长素可诱导性(McClure &Guilfoyle，1989)。
实施例6：侧根起始中的新基因：转录因子
由于转录因子在图式形成和发育中起关键作用(Sabatini等人，2003)，所以显然我们的选择中此类基因(19)在侧根起始期间信号级联中将是尤其重要的(表1)。该选择中多数基因在最近通过拟南芥根尖的转录物序型分析研究中表明在中柱组织(包括中柱鞘)中被特异表达(Birnbaum等人，2003)，证明我们的研究中使用的选择标准是合理的。
有趣的是，两个AP2结构域转录因子属于相同的亚进化枝。这暗示这些基因可能具有冗余功能。此外，有一个AP2结构域转录因子属于三种基因组成的相同的亚进化枝，其没有在微阵列上代表(Alonso等人，2003)。我们假设该基因(At4g27950)也可以与该亚进化枝的两种其他成员一样是功能冗余的。因此，将该基因加入我们的选择中，使我们选择的最后数目达到20种基因。
在我们的数据集中，有15种转录因子在生长素信号传递中的角色还没有被提出。关于验证的开始，最重要的是对这些转录因子在侧根起始方面进行功能分析。
许多这些转录因子很有可能涉及侧根发育，因为已经报导了它们的同源物在器官发育中的角色。ABI3基因以前被描述为种子特异性基因，但是最近已经表明它在生长素信号传递和侧根发育中起作用(Brady等人，2003)。而且，已经表明AP2和几种同源异型框基因涉及花器官发育，其暗示同源物很可能在其他器官如侧根的发育中必要的(Carpenter & Coen，1990；Maes等人，1999)。
有趣的是，有一种转录因子MYB124，其突变体具有异常的气孔发育。由于所述突变，形成了具有四个而不是两个保卫细胞的气孔(Yang & Sack，1995)。当用生长素处理根时它的上调暗示MYB124基因产物与它在气孔发育中的一样可能在中柱鞘的形成分裂(侧根起始)中具有关键作用。
实施例7：鉴定不对称细胞分裂基因
使用LRI作为模型以在遗传学上仔细分析不对称细胞分裂，我们在我们的10个聚簇中进行了研究，所述聚簇含有该类型分裂的推定的调节子。
首先，我们分析哪个聚簇与G2到M转变强烈连锁，通过为同步化的拟南芥细胞悬浮液使用基因组范围表达数据研究细胞周期进展期间的表达谱进行所述分析(Menges等人，2003)。我们发现聚簇3中48％的基因在G2到M转变达到峰值。这与在所有其他聚簇中少于10％的基因在该转变达到峰值相反。与其他聚簇相比，这是该聚簇中G2到M转变的强烈过量代表。此外，这占整个数据集中存在的G2到M特异性基因的59％。
其次，我们分析了哪个聚簇可能与不对称细胞分裂相关。为此，我们使用被分配功能类别的基因的蛋白质序列(http://www.godatabase.org/cgi-bin/amigo/go.cgi)多种生物(如线虫(Caenorhabditis elegans)、黑腹果蝇(Drosophila melanogaste)、栗酒裂殖酵母(Schizosaccharomyces pombe)、小鼠...)中“不对称细胞分裂”和/或“细胞极性的建立和/或保持”。我们用多种生物的蛋白质序列和被分配给我们的不同聚簇的基因的那些拟南芥蛋白质序列进行了蛋白质blast分析。这导致聚簇3中推定与“不对称细胞分裂”、“细胞命运定型”和/或“细胞极性的建立和/或保持”相关的54种基因的过量代表。
为了进一步分析不对称细胞分裂过程，我们因此集中于聚簇3内的340种基因。在该聚簇中，已经描述了25％的基因；剩余的75％是未知的、表达的、假设的或推定的。
为了进一步减少感兴趣的候选者数目；我们扣除了涉及正常细胞分裂的那些基因(同步化的、正分裂的拟南芥细胞悬浮细胞)(Menges等人，2003)。该分析后，我们以潜在涉及不对称细胞分裂的190个候选者告终。
实施例8：用于改进结果的元分析(meta-analysis)
以前已经阐明中柱鞘中细胞周期进展对于拟南芥中SOLITARYROOT/IAA14介导的侧根起始不是足够的(Vanneste等人，2005)。
为了支持该发现，我们分析了过表达(35S)细胞周期基因的转基因株系的根表型。基于Himanen等人(2002)中所示和我们的数据集中的侧根诱导时细胞周期阶段特异性表达谱，我们选择了CYCD3；1(G1-向-S和G2-向-M，Dewitte和Murray，2003)，E2Fa/DPa(G1-向-S，De Veylder等人，2002)和CDKB1；1(G2-向-M，Boudolf等人，2004)。分析了所有株系的10天龄幼苗中的过表达表型并且与WT比较(图5)。没有一个转基因株系显示出侧根密度的显著增加。E2Fa/DPa的双转基因过表达株系甚至显示出与野生型Col相比侧根数目的强烈减少。CDKB1；1的过表达也导致较少的侧根。此外，对于CDKB1；1的情况，CDKB1；1(CDKB1；1.N161)(Boudolf等人，2004)的显性失活等位基因的过表达导致侧根数目的更强烈的减少。
接着，我们分析了生长素(NAA)应用与增加的细胞周期基因表达相组合是否可以导致与仅仅生长素或细胞周期相比更多的侧根数目。我们因此转移了E2FaOE、DPaOEOE、CYCD3；1OE和CDKB1；1OE的上述转基因拟南芥株系的5天龄幼苗以增加生长素浓度(10-8、10-7和10-6M NAA)并另外生长5天后分析它们起始侧根的能力。我们发现与野生型相比CYCD3；1OE的显著增加。类似地，在E2Fa/DPaOE转基因株系中LRs/cm的数目可以显著增加，直到超过高生长素浓度下的野生型数目。甚至CDKB1；1OE也超过了生长素应用时的WT数目，而CDKB1；1DNOE株系则不是这样。
上面的结果表明刺激基本细胞周期机器对于从头侧根起始不是足够的，但是当提供额外的生长素时，可以利用增强的细胞周期能力来产生新的器官。这确证了Vanneste等人(2005)的意见，即细胞周期激活后，需要另一种因子来特异性驱动侧根起始。
尽管CDKB1；1在侧根起始中的推定的功能，但是细胞周期基因显然不是侧根起始的关键调节子。所以，我们在我们的数据集中通过元分析搜索侧根起始的潜在特异调节子。进行该元分析以将基因数目从1920种重要的基因减少到15种非常重要的候选者(表3)。该分析涉及随后的重叠步骤和基因子集的深度分析，如下述。
1)Affymetrix拟南芥ATH-1基因组阵列(22758种基因)；
2)独特的显著差别表达的基因(1920)；
3)基于下面的标准使用深度分析使用功能类别术语选择1个聚簇后在侧根起始期间不对称细胞分裂中被上调的基因(340)：
＊最高的％G2-M基因
＊最高的％涉及不对称的基因
＊最高的％涉及极性的基因
＊最高的％涉及细胞命运的基因；
4)基于Menges等人(2003)扣除有丝分裂器后潜在涉及细胞命运和细胞极性的基因(190)；
5)与剩余的190种基因重叠后涉及在侧根起始期间木质部极中柱鞘中生长素诱导的细胞命运和/或细胞极性的基因(15)与取决于快速SLR/IAA14降解用于正常生长素应答的那些侧根起始基因(913)，如来自交叉表(图4)。
实施例9：BDL涉及侧根起始
如以前确定的(Vanneste等人，2005)，侧根起始的一种重要的调节子机理是生长素信号传递和转运。表4列出了涉及那些事件的基因并且表明它们多数在早期被上/下调。已经表明许多基因涉及侧根形成(ALF1/RTY/SUR1，Celenza等人，1995，King等人，1995，Boerjan等人，1995；DFL1，Nakazawa等人，2001)，并且对于几种Aux/IAAs和ARFs，在以前表明了在侧根起始和/或形成中的角色(IAA19/MSG2，Tatematsu等人，2004；ARF19，Wilmoth等人，2004；IAA1/AXR5，Yang等人，2004；IAA3/SHY2，Tian和Reed，1999)。
对于BDL/IAA12(与MP/ARF5的一对转录调节子的部分)，我们阐明其涉及侧根起始。BDL蛋白质的已经建立的突变形式在J0121xUAS：bdl中的木质部极中柱特异性表达(0.0±0.0)导致无侧根的表型，而对照株系Col-0(3.0±0.1)、J0121(4.1±0.1)、UAS：bdl(3.1±0.1)、UAS：BDL(2.8±0.1)和J0121xUAS：BDL(3.6±0.1)没有显示出每cm侧根数目的减少(图6)。
实施例10：CYCA2；4在侧根形成中的作用
CYCA2；4被鉴定为侧根起始期间细胞分裂的推定的重要调节子(Vanneste等人，2005)。然而，CYCA2；4的过表达不诱导侧根的增加(类似于其他细胞周期基因的过表达)，而它刺激细胞周期前进，如通过子叶中核内再复制水平的强烈降低所例证。而且在敲除体中，我们没有观察到侧根密度的明显改变。但是，CYCA2；4属于由4个成员组成的小基因家族。组合该家族的多种成员中的突变导致侧根密度的显著降低(图7)。这些数据一起表明A2型细胞周期蛋白是所需的，但是对于发生侧根起始不是足够的。
有趣的是，CYCA2；4-一种核心细胞周期基因-在元分析后保持在基因列表中。不幸的是，在过表达株系中侧根表型的缺少可能提示需要基因/因子的组合来特异驱动不对称细胞分裂和侧根起始。当组合时将诱导侧根起始的最可能的候选者在实施例8鉴定的15种基因的子集内。
实施例11：突变体筛选
使来自多种聚簇中基因的许多SALK T-DNA插入突变体变得纯合并分析它们的侧根表型(图8)。在该图中，具有侧根数目显著增加或减少的突变体的条形分别为绿色或红色。该图部分周围的绿色或红色框分别指出来自上调或下调聚簇的基因。
除了侧根表型外，还检测了需要不对称细胞分裂的其他过程，如气孔形成和胚发生中的缺陷。
实施例12：通过GUS/GFP融合的表达分析
使来自多种聚簇中基因的许多启动子-GUS/GFP融合变得纯合并且详细分析它们的表达图式(图9)。GUS/GFP表达图式主要与微阵列数据集中基因的上调或下调相一致。
对于4种基因，详细分析了表达图式，并且揭示了在不对称细胞分裂时侧根起始位点中GUS/GFP的特异上调或下调与微阵列中检测到的转录物水平相一致(图10)。
表
表1：过滤后功能类别中的偏移概述
| 功能类别 | 3110中％ | 266中％ |
| 细胞周期/DNA合成 | 3.9 | 8.3 |
| 激酶/磷酸酶 | 5.5 | 3.4 |
| 代谢/能量 | 22.7 | 12.4 |
| 蛋白质合成/降解 | 9.7 | 9.0 |
| RNA加工 | 1.6 | 3.0 |
| 信号传递/发育 | 6.8 | 12.8 |
| 胁迫 | 4.0 | 2.3 |
| 转录活性 | 10.9 | 14.7 |
| 运输 | 7.2 | 2.3 |
| 其他 | 5.5 | 7.1 |
| 未分类的/未知 | 22.1 | 24.4 |
以“侧根起始“基因加密的侧根起始
表2：在突变体(slr)中不应答的被上调的转录因子列表
表3.在木质部极中柱鞘中被上调、在slr突变体中不应答和可能涉及不对称细胞分裂的基因列表
| AGI代码 | 描述 |
| AT5G63950 | 含有SNF2结构域的蛋白质/含有解链酶结构域的蛋白质 |
| AT5G67100 | DNA-指导的DNA聚合酶α催化亚基，推定的 |
| AT2G33620 | DNA-结合家族蛋白/AT-HOOK蛋白1(AHP1) |
| AT2G46990 | 生长素应答蛋白/吲哚乙酸诱导的蛋白20(IAA20) |
| AT5G47440 | 表达的非常类似于未知蛋白的蛋白质 |
| AT4G32460 | 表达的蛋白质 |
| AT4G13210 | 果胶酸裂合酶家族蛋白 |
| AT3G59430 | 表达的蛋白质 |
| AT3G01070 | 含有质体蓝素样结构域的蛋白质 |
| AT3G59420 | 受体蛋白激酶，推定的(ACR4) |
| AT1G80370 | 细胞周期蛋白，推定的 |
| AT1G69530 | 扩展蛋白，推定的(EXP1) |
| AT4G02060 | PROLIFERA蛋白(PRL)/DNA复制许可因子MCM7(MCM7) |
| AT5G67070 | 快速碱化因子(RALF)家族蛋白 |
| AT1G61580 | 60S核糖体蛋白L3(RPL3B) |
表4：如通过细胞分选方法确定的涉及生长素信号传递和运输的早期基因中的表达
| 基因名称(s) | agi | 0h | 2h | 6h |
| AAP6 | At5g49630 | 0,852584 | 1,25473 | 1,549922 |
| ALF1SUR1 RTY | AT2g20610 | 3,526627 | 1,659614 | 1,132184 |
| ARF16 | At4g30080 | 0,727253 | 1,044252 | 1,222434 |
| ARF18 | At3g61830 | 1,02858 | 1,042211 | 1,37744 |
| ARF19 | At1g19220 | 0,964729 | 2,260746 | 1,345781 |
| ARF4 | At5g60450 | 0,993832 | 2,433736 | 1,34132 |
| ARF5IAA24 MP | At1g19850 | 0,805484 | 1,062866 | 1,646489 |
| AtGH3_1 | At2g14960 | 0,993763 | 14,46607 | 12,64589 |
| AtGH3_4 | At1g59500 | 1,832319 | 11,94342 | 5,062107 |
| AtGH3_5 | AT4g27260 | 4,933415 | 36,44469 | 28,02028 |
| AtGH3_6DFL1 | At5g54510 | 4,107676 | 33,98892 | 33,18595 |
| ATSAUR32 | At2g46690 | 0,745399 | 1,083697 | 1,357203 |
| ATSAUR51 | At1g75580 | 0,641888 | 0,861681 | 1,394927 |
| DFL1 | AT5G54510 | 4,107676 | 33,98892 | 33,18595 |
| HAT2 | AT5G47370 | 3,866166 | 5,905478 | 1,72286 |
| IAA1 | AT4G14560 | 1,444098 | 2,650251 | 3,498028 |
| IAA11 | AT4G28640 | 1,081115 | 2,990535 | 3,893183 |
| IAA12BDL | AT1G04550 | 1,070244 | 2,483024 | 2,141742 |
| IAA13 | AT2G33310 | 3,386846 | 16,95071 | 17,15706 |
| IAA17AXR3 | AT1G04250 | 4,20049 | 5,560019 | 1,337217 |
| IAA19MSG2 | AT3G15540 | 8,950041 | 37,74343 | 43,64249 |
| IAA20 | AT2G46990 | 0,643367 | 0,719127 | 1,830361 |
| IAA26PAP1 | AT3G16500 | 1,67767 | 4,3412 | 2,185664 |
| IAA29 | AT4G32280 | 1,230655 | 15,62485 | 12,44667 |
| IAA3SHY2 | AT1G04240 | 0,649735 | 1,143323 | 0,778411 |
| IAA5 | AT1G15580 | 0,800279 | 1,701733 | 2,127686 |
| PINOID-样 | At3g44610 | 0,730512 | 0,863134 | 2,114596 |
表5：使用的突变体代码
| 图上的代码 | AGI |
| H19 | At3g59850 |
| R | At4g23750 |
| S | At4g23750 |
| H12 | At4g38210 |
| H10 | At5g15080 |
| 101 | At5g51560 |
| KO1 | At1g11140 |
| KO2 | At1g55580 |
| 90 | At1g57820 |
| H8 | At1g69530 |
| 图上的代码 | AGI |
| 108 | At1g72250 |
| H2 | At2g06850 |
| 67 | At2g22610 |
| 68 | At2g28620 |
| 94 | At2g28620 |
| 135 | AT2G33620 |
| 59 | At3g51280 |
| 87 | At3g51740 |
| H14 | At3g53190 |
| 83 | At4g02150 |
| 85 | At4g05190 |
| 105 | At4g18570 |
| 91 | At4g21820 |
| H11 | At4g29360 |
| 75 | At4g32830 |
| 106 | At4g32830 |
| H23 | At5g08000 |
| 88 | At5g45780 |
| 124 | At5g47440 |
| 65 | At5g48460 |
| H3a | At1g08840 |
| M | At1g72310 |
| H4 | At1g75640 |
| 5 | At4g28640 |
| H15 | At1g64390 |
| H20 | At3g58040 |
| 图上的代码 | AGI |
| H21 | At1g14720 |
| H6 | At1g29050 |
| H29 | At1g53500 |
| H1 | At1g60610 |
| H26 | At2g45470 |
| H28 | At3g10810 |
| H5 | At3g52370 |
| H18 | At4g03960 |
| H9 | At4g14130 |
| H27 | At5g18650 |
| H13 | At5g38895 |
| H17 | At5g54160 |
| H16 | At5g57740 |
| H22 | At5g03650 |
| 6 | At2g33830 |
| 22 | At2g33830 |
| H7 | At3g54920 |
| H24 | At3g54920 |
表6：用于GUS融合中的基因
| 1 | pAt4g13770::GUS |
| 2 | pAT2G20610::GUS |
| 3 | pAt3g46130::GUS |
| 4 | pAt3g11280::GUS |
| 5 | pAt1g49740::GUS |
| 6 | pAt1g64405::GUS |
| 7 | pAt3g55620::GUS |
| 8 | pCYCB1；1::GUS |
| 9 | pCYCB1；3::GUS |
| 10 | pCYCB2；2::GUS |
| 11 | pCYCB2；4::GUS |
| 12 | pCYCA2；2::GUS |
| 13 | pCYCA2；3::GUS |
| 14 | pCDKB2；2::GUS |
| 15 | pCKS2::GUS |
| 16 | pDEL3::GUS |
| 17 | pAt3g58100::GUS |
| 18 | pAt1g54990::GUS |
| 19 | pAt5g26930::GUS |
| 20 | pAT1G69530::GUS |
| 21 | pACR4＞＞H2B::YFP(Gifford等人，2003) |
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序列表
<110>VIB VZW
国立比利时根特大学
<120>涉及不对称细胞分裂的基因
<130>TBE/Lat/V214
<150>EP05107830.1
<151>2005-08-26
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Lys Asp Pro Trp Ile Ile Lys Lys Val Phe His Phe Ala Ser Val Leu
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Glu Ser Pro Glu Asn Ala Glu Glu Ser Leu Val Ser Leu Asp Leu Cys
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Phe Tyr Asp Lys Thr Trp Pro His Asp Pro Asn Val Ala Tyr Asn Lys
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Val Ala
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<221>MISC_FEATURE
<223>At4g23750
<400>3
Met Glu Ala Glu Lys Lys Met Val Leu Pro Arg Ile Lys Phe Thr Glu
1 5 10 15
His Lys Thr Asn Thr Thr Thr Ile Val Ser Glu Leu Thr Asn Thr His
20 25 30
Gln Thr Arg Ile Leu Arg Ile Ser Val Thr Asp Pro Asp Ala Thr Asp
35 40 45
Ser Ser Ser Asp Asp Glu Glu Glu Glu His Gln Arg Phe Val Ser Lys
50 55 60
Arg Arg Arg Val Lys Lys Phe Val Asn Glu Val Tyr Leu Asp Ser Gly
65 70 75 80
Ala Val Val Thr Gly Ser Cys Gly Gln Met Glu Ser Lys Lys Arg Gln
85 90 95
Lys Arg Ala Val Lys Ser Glu Ser Thr Val Ser Pro Val Val Ser Ala
100 105 110
Thr Thr Thr Thr Thr Gly Glu Lys Lys Phe Arg Gly Val Arg Gln Arg
115 120 125
Pro Trp Gly Lys Trp Ala Ala Glu Ile Arg Asp Pro Leu Lys Arg Val
130 135 140
Arg Leu Trp Leu Gly Thr Tyr Asn Thr Ala Glu Glu Ala Ala Met Val
145 150 155 160
Tyr Asp Asn Ala Ala Ile Gln Leu Arg Gly Pro Asp Ala Leu Thr Asn
165 170 175
Phe Ser Val Thr Pro Thr Thr Ala Thr Glu Lys Lys Ala Pro Pro Pro
180 185 190
Ser Pro Val Lys Lys Lys Lys Lys Lys Asn Asn Lys Ser Lys Lys Ser
195 200 205
Val Thr Ala Ser Ser Ser Ile Ser Arg Ser Ser Ser Asn Asp Cys Leu
210 215 220
Cys Ser Pro Val Ser Val Leu Arg Ser Pro Phe Ala Val Asp Glu Phe
225 230 235 240
Ser Gly Ile Ser Ser Ser Pro Val Ala Ala Val Val Val Lys Glu Glu
245 250 255
Pro Ser Met Thr Thr Val Ser Glu Thr Phe Ser Asp Phe Ser Ala Pro
260 265 270
Leu Phe Ser Asp Asp Asp Val Phe Asp Phe Arg Ser Ser Val Val Pro
275 280 285
Asp Tyr Leu Gly Gly Asp Leu Phe Gly Glu Asp Leu Phe Thr Ala Asp
290 295 300
Met Cys Thr Asp Met Asn Phe Gly Phe Asp Phe Gly Ser Gly Leu Ser
305 310 315 320
Ser Trp His Met Glu Asp His Phe Gln Asp Ile Gly Asp Leu Phe Gly
325 330 335
Ser Asp Pro Leu Leu Ala Val
340
<210>4
<211>348
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5g18560
<400>4
Met Ser Thr Ser Lys Thr Leu Asp His Asn Lys Pro Phe Glu Thr Ser
1 5 10 15
Gln Thr Gln Met Gly Phe Ala Leu Ile His Gln Asn Thr Ser Ala Asn
20 25 30
Thr Thr Thr Thr Thr Thr Thr Gly Glu Arg Arg Gly Arg Arg Ser Lys
35 40 45
Gln Ala Glu Pro Gly Arg Phe Leu Gly Val Arg Arg Arg Pro Trp Gly
50 55 60
Arg Tyr Ala Ala Glu Ile Arg Asp Pro Thr Thr Lys Glu Arg His Trp
65 70 75 80
Leu Gly Thr Phe Asp Thr Ala His Glu Ala Ala Leu Ala Tyr Asp Arg
85 90 95
Ala Ala Leu Ser Met Arg Gly Thr Gln Ala Arg Thr Asn Phe Val Tyr
100 105 110
Thr Pro Thr Asp Val His Thr Ile Leu Thr Asn Pro Asn Leu His Ser
115 120 125
Leu Ile Val Ser Pro Tyr Asn Asn Asn Gln Ser Phe Leu Pro Asn Ser
130 135 140
Ser Pro Gln Phe Val Ile Asp His His Pro His Tyr Gln Asn Tyr His
145 150 155 160
Gln Pro Gln Gln Pro Lys His Thr Leu Pro Gln Thr Val Leu Pro Ala
165 170 175
Ala Ser Phe Lys Thr Pro Val Arg His Gln Ser Val Asp Ile Gln Ala
180 185 190
Phe Gly Asn Ser Pro Gln Asn Ser Ser Ser Asn Gly Ser Leu Ser Ser
195 200 205
Ser Leu Asp Glu Glu Asn Asn Phe Phe Phe Ser Leu Thr Ser Glu Glu
210 215 220
His Asn Lys Ser Asn Asn Asn Ser Gly Tyr Leu Asp Cys Ile Val Pro
225 230 235 240
Asn His Cys Leu Lys Pro Pro Pro Glu Ala Thr Thr Thr Gln Asn Gln
245 250 255
Ala Gly Ala Ser Phe Thr Thr Pro Val Ala Ser Lys Ala Ser Glu Pro
260 265 270
Tyr Gly Gly Phe Ser Asn Ser Tyr Phe Glu Asp Gly Glu Met Met Met
275 280 285
Met Asn His His Glu Phe Gly Ser Cys Asp Leu Ser Ala Met Ile Thr
290 295 300
Asn Tyr Gly Ala Ala Ala Ala Ser Met Ser Met Glu Asp Tyr Gly Met
305 310 315 320
Met Glu Pro Gln Asp Leu Ser Ser Ser Ser Ile Ala Ala Phe Gly Asp
325 330 335
Val Val Ala Asp Thr Thr Gly Phe Tyr Ser Val Phe
340 345
<210>5
<211>189
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At1g28360
<400>5
Met Ala Ser Thr Thr Cys Ala Arg Glu Val His Tyr Arg Gly Val Arg
1 5 10 15
Lys Arg Pro Trp Gly Arg Tyr Ala Ala Glu Ile Arg Asp Pro Trp Lys
20 25 30
Lys Thr Arg Val Trp Leu Gly Thr Phe Asp Thr Pro Glu Glu Ala Ala
35 40 45
Leu Ala Tyr Asp Gly Ala Ala Arg Phe Leu Arg Gly Ile Lys Ala Lys
50 55 60
Thr Asn Phe Pro Ser Pro Leu Ser Leu Asp Leu Asn His Leu Pro Ser
65 70 75 80
Ala Pro Ser Ala Ala Thr Ala Ala Ala Asn Asn Gln Pro His Gln His
85 90 95
Gln Gln Leu Trp Phe Ala Ala Pro Pro Pro Val Pro Pro Ser Ser Asp
100 105 110
His His His Gln His His Arg Ile Phe Leu Arg Thr Gly Val Leu Asn
115 120 125
Asp Lys Thr Ser Asp Tyr Ser Ser Thr Glu Ala Pro Leu Tyr Phe Thr
130 135 140
Ser Ser Pro Asn Thr Ala Thr Ser Ser Pro Gly Tyr Gln Val Val Gly
145 150 155 160
Phe Pro Met Met Asn Ser Ser Pro Ser Pro Val Thr Val Arg Arg Gly
165 170 175
Leu Ala Ile Asp Leu Asn Glu Pro Pro Pro Leu Trp Leu
180 185
<210>6
<211>566
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5g10510
<400>6
Met Glu Met Leu Arg Ser Ser Asp Gln Ser Gln Phe Val Ser Tyr Asp
1 5 10 15
Ala Ser Ser Ala Ala Ser Ser Ser Pro Tyr Leu Leu Asp Asn Phe Tyr
20 25 30
Gly Trp Ser Asn Gln Lys Pro Gln Glu Phe Phe Lys Glu Glu Ala Gln
35 40 45
Leu Ala Ala Ala Ala Ser Met Ala Asp Ser Thr Ile Leu Thr Thr Phe
50 55 60
Val Asp Pro Gln Ser His His Ser Gln Asn His Ile Pro Lys Leu Glu
65 70 75 80
Asp Phe Leu Gly Asp Ser Ser Ser Ile Val Arg Tyr Ser Asp Asn Ser
85 90 95
Gln Thr Asp Thr Gln Asp Ser Ser Leu Thr Gln Ile Tyr Asp Pro Arg
100 105 110
His His His Asn Gln Thr Gly Phe Tyr Ser Asp His His Asp Phe Lys
115 120 125
Thr Met Ala Gly Phe Gln Ser Ala Phe Ser Thr Asn Ser Gly Ser Glu
130 135 140
Val Asp Asp Ser Ala Ser Ile Gly Arg Thr His Leu Ala Gly Asp Tyr
145 150 155 160
Leu Gly His Val Val Glu Ser Ser Gly Pro Glu Leu Gly Phe His Gly
165 170 175
Gly Ser Thr Gly Ala Leu Ser Leu Gly Val Asn Val Asn Asn Asn Thr
180 185 190
Asn His Arg Asn Asp Asn Asp Asn His Tyr Arg Gly Asn Asn Asn Gly
195 200 205
Glu Arg Ile Asn Asn Asn Asn Asn Asn Asp Asn Glu Lys Thr Asp Ser
210 215 220
Glu Lys Glu Lys Ala Val Val Ala Val Glu Thr Ser Asp Cys Ser Asn
225 230 235 240
Lys Lys Ile Ala Asp Thr Phe Gly Gln Arg Thr Ser Ile Tyr Arg Gly
245 250 255
Val Thr Arg His Arg Trp Thr Gly Arg Tyr Glu Ala His Leu Trp Asp
260 265 270
Asn Ser Cys Arg Arg Glu Gly Gln Ala Arg Lys Gly Arg Gln Gly Gly
275 280 285
Tyr Asp Lys Glu Asp Lys Ala Ala Arg Ala Tyr Asp Leu Ala Ala Leu
290 295 300
Lys Tyr Trp Asn Ala Thr Ala Thr Thr Asn Phe Pro Ile Thr Asn Tyr
305 310 315 320
Ser Lys Glu Val Glu Glu Met Lys His Met Thr Lys Gln Glu PheIle
325 330 335
Ala Ser Leu Arg Arg Lys Ser Ser Gly Phe Ser Arg Gly Ala Ser Ile
340 345 350
Tyr Arg Gly Val Thr Arg His His Gln Gln Gly Arg Trp Gln Ala Arg
355 360 365
Ile Gly Arg Val Ala Gly Asn Lys Asp Leu Tyr Leu Gly Thr Phe Ala
370 375 380
Thr Glu Glu Glu Ala Ala Glu Ala Tyr Asp Ile Ala Ala Ile Lys Phe
385 390 395 400
Arg Gly Ile Asn Ala Val Thr Asn Phe Glu Met Asn Arg Tyr Asp Val
405 410 415
Glu Ala Ile Met Lys Ser Ala Leu Pro Ile Gly Gly Ala Ala Lys Arg
420 425 430
Leu Lys Leu Ser Leu Glu Ala Ala Ala Ser Ser Glu Gln Lys Pro Ile
435 440 445
Leu Gly His His Gln Leu His His Phe Gln Gln Gln Gln Gln Gln Gln
450 455 460
Gln Leu Gln Leu Gln Ser Ser Pro Asn His Ser Ser Ile Asn Phe Ala
465 470 475 480
Leu Cys Pro Asn Ser Ala Val Gln Ser Gln Gln Ile Ile Pro Cys Gly
485 490 495
Ile Pro Phe Glu Ala Ala Ala Leu Tyr His His His Gln Gln Gln Gln
500 505 510
Gln His Gln Gln Gln Gln Gln Gln Gln Asn Phe Phe Gln His Phe Pro
515 520 525
Ala Asn Ala Ala Ser Asp Ser Thr Gly Ser Asn Asn Asn Ser Asn Val
530 535 540
Gln Gly Thr Met Gly Leu Met Ala Pro Asn Pro Ala Glu Phe Phe Leu
545 550 555 560
Trp Pro Asn Gln Ser Tyr
565
<210>7
<211>555
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5g57390
<400>7
Met Lys Asn Asn Asn Asn Lys Ser Ser Ser Ser Ser Ser Tyr Asp Ser
1 5 10 15
Ser Leu Ser Pro Ser Ser Ser Ser Ser Ser His Gln Asn Trp Leu Ser
20 25 30
Phe Ser Leu Ser Asn Asn Asn Asn Asn Phe Asn Ser Ser Ser Asn Pro
35 40 45
Asn Leu Thr Ser Ser Thr Ser Asp His His His Pro His Pro Ser His
50 55 60
Leu Ser Leu Phe Gln Ala Phe Ser Thr Ser Pro Val Glu Arg Gln Asp
65 70 75 80
Gly Ser Pro Gly Val Ser Pro Ser Asp Ala Thr Ala Val Leu Ser Val
85 90 95
Tyr Pro Gly Gly Pro Lys Leu Glu Asn Phe Leu Gly Gly Gly Ala Ser
100 105 110
Thr Thr Thr Thr Arg Pro Met Gln Gln Val Gln Ser Leu Gly Gly Val
115 120 125
Val Phe Ser Ser Asp Leu Gln Pro Pro Leu His Pro Pro Ser Ala Ala
130 135 140
Glu Ile Tyr Asp Ser Glu Leu Lys Ser Ile Ala Ala Ser Phe Leu Gly
145 150 155 160
Asn Tyr Ser Gly Gly His Ser Ser Glu Val Ser Ser Val His Lys Gln
165 170 175
Gln Pro Asn Pro Leu Ala Val Ser Glu Ala Ser Pro Thr Pro Lys Lys
180 185 190
Asn Val Glu Ser Phe Gly Gln Arg Thr Ser Ile Tyr Arg Gly Val Thr
195 200 205
Arg His Arg Trp Thr Gly Arg Tyr Glu Ala His Leu Trp Asp Asn Ser
210 215 220
Cys Arg Arg Glu Gly Gln Ser Arg Lys Gly Arg Gln Gly Gly Tyr Asp
225 230 235 240
Lys Glu Asp Lys Ala Ala Arg Ala Tyr Asp Leu Ala Ala Leu Lys Tyr
245 250 255
Trp Gly Pro Thr Thr Thr Thr Asn Phe Pro Ile Ser Asn Tyr Glu Ser
260 265 270
Glu Leu Glu Glu Met Lys His Met Thr Arg Gln Glu Phe Val Ala Ser
275 280 285
Leu Arg Arg Lys Ser Ser Gly Phe Ser Arg Gly Ala Ser Met Tyr Arg
290 295 300
Gly Val Thr Arg His His Gln His Gly Arg Trp Gln Ala Arg Ile Gly
305 310 315 320
Arg Val Ala Gly Asn Lys Asp Leu Tyr Leu Gly Thr Phe Ser Thr Gln
325 330 335
Glu Glu Ala Ala Glu Ala Tyr Asp Ile Ala Ala Ile Lys Phe Arg Gly
340 345 350
Leu Asn Ala Val Thr Asn Phe Asp Ile Ser Arg Tyr Asp Val Lys Ser
355 360 365
Ile Ala Ser Cys Asn Leu Pro Val Gly Gly Leu Met Pro Lys Pro Ser
370 375 380
Pro Ala Thr Ala Ala Ala Asp Lys Thr Val Asp Leu Ser Pro Ser Asp
385 390 395 400
Ser Pro Ser Leu Thr Thr Pro Ser Leu Thr Phe Asn Val Ala Thr Pro
405 410 415
Val Asn Asp His Gly Gly Thr Phe Tyr His Thr Gly Ile Pro Ile Lys
420 425 430
Pro Asp Pro Ala Asp His Tyr Trp Ser Asn Ile Phe Gly Phe Gln Ala
435 440 445
Asn Pro Lys Ala Glu Met Arg Pro Leu Ala Asn Phe Gly Ser Asp Leu
450 455 460
His Asn Pro Ser Pro Gly Tyr Ala Ile Met Pro Val Met Gln Glu Gly
465 470 475 480
Glu Asn Asn Phe Gly Gly Ser Phe Val Gly Ser Asp Gly Tyr Asn Asn
485 490 495
His Ser Ala Ala Ser Asn Pro Val Ser Ala Ile Pro Leu Ser Ser Thr
500 505 510
Thr Thr Met Ser Asn Gly Asn Glu Gly Tyr Gly Gly Asn Ile Asn Trp
515 520 525
Ile Asn Asn Asn Ile Ser Ser Ser Tyr Gln Thr Ala Lys Ser Asn Leu
530 535 540
Ser Val Leu His Thr Pro Val Phe Gly Leu Glu
545 550 555
<210>8
<211>246
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At4g28640
<400>8
Met Glu Gly Gly Ser Ala Ser Gly Ser Ala Ser Ala Leu Ser Asn Asp
1 5 10 15
Glu Asn Leu Val Val Ser Cys Glu Asp Ser Ser Ser Pro Ile Gly Asn
20 25 30
Glu Leu Glu Leu Gly Leu Thr Leu Ser Leu Gly Arg Lys Gly Tyr Arg
35 40 45
Asp Cys Arg Val Tyr Ala Asp Asp Ser Ser Ser Ser Ser Ser Ser Ser
50 55 60
Ser Leu Ser Arg Ala Ser Val Ile Ala Gly Ile Lys Arg Thr Ala Asp
65 70 75 80
Ser Met Ala Ala Thr Ser Gly Gln Val Val Gly Trp Pro Pro Ile Arg
85 90 95
Thr Tyr Arg Met Asn Ser Met Val Asn Gln Ala Lys Ala Ser Ala Thr
100 105 110
Glu Asp Pro Asn Leu Glu Ile Ser Gln Ala Val Asn Lys Asn Arg Ser
115 120 125
Asp Ser Thr Lys Met Arg Asn Ser Met Phe Val Lys Val Thr Met Asp
130 135 140
Gly Ile Pro Ile Gly Arg Lys Ile Asp Leu Asn Ala His Lys Cys Tyr
145 150 155 160
Glu Ser Leu Ser Asn Thr Leu Glu Glu Met Phe Leu Lys Pro Lys Leu
165 170 175
Gly Ser Arg Thr Leu Glu Thr Asp Gly His Met Glu Thr Pro Val Lys
180 185 190
Ile Leu Pro Asp Gly Ser Ser Gly Leu Val Leu Thr Tyr Glu Asp Lys
195 200 205
Glu Gly Asp Trp Met Leu Val Gly Asp Val Pro Trp Gly Met Phe Ile
210 215 220
Gly Ser Val Arg Arg Leu Arg Ile Met Lys Thr Ser Glu Ala Thr Gly
225 230 235 240
Lys Ala Gln Met Ile Leu
245
<210>9
<211>251
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At4g32280
<400>9
Met Glu Leu Asp Leu Gly Leu Ser Leu Ser Pro His Lys Ser Ser Lys
1 5 10 15
Leu Gly Phe Asn Phe Asp Leu Asn Lys His Cys Ala Ile Glu Gly Ala
20 25 30
Ala Ser Cys Leu Gly Thr Glu Lys Leu Arg Phe Glu Ala Thr Phe Gly
35 40 45
Leu Gly Asn Val Glu Glu Asn Cys Tyr Met Pro Lys Gln Arg Leu Phe
50 55 60
Ala Leu Asn Gly Gln Pro Asn Glu Glu Asp Glu Asp Pro Leu Glu Ser
65 70 75 80
Glu Ser Ser Ile Val Tyr Asp Asp Glu Glu Glu Asn Ser Glu Val Val
85 90 95
Gly Trp Pro Pro Val Lys Thr Cys Met Ile Lys Tyr Gly Ser Tyr His
100 105 110
His Arg His Ile Arg Asn His His His Cys Pro Tyr His His Arg Gly
115 120 125
Arg Arg Ile Thr Ala Met Asn Asn Asn Ile Ser Asn Pro Thr Thr Ala
130 135 140
Thr Val Gly Ser Ser Ser Ser Ser Ser Ile Ser Ser Arg Ser Ser Met
145 150 155 160
Tyr Val Lys Val Lys Met Asp Gly Val Ala Ile Ala Arg Lys Val Asp
165 170 175
Ile Lys Leu Phe Asn Ser Tyr Glu Ser Leu Thr Asn Ser Leu Ile Thr
180 185 190
Met Phe Thr Glu Tyr Glu Asp Cys Asp Arg Glu Asp Thr Asn Tyr Thr
195 200 205
Phe Thr Phe Gln Gly Lys Glu Gly Asp Trp Leu Leu Arg Gly Asp Val
210 215 220
Thr Trp Lys Ile Phe Ala Glu Ser Val His Arg Ile Ser Ile Ile Arg
225 230 235 240
Asp Arg Pro Cys Ala Tyr Thr Arg Cys Leu Phe
245 250
<210>10
<211>172
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At3g62100
<400>10
Met Gly Arg Gly Arg Ser Ser Ser Ser Ser Ser Ile Glu Ser Ser Cys
1 5 10 15
Lys Ser Asn Pro Phe Gly Val Ser Ser Ser Asn Thr Arg Asn Leu Ser
20 25 30
Thr Asp Leu Arg Leu Gly Leu Ser Phe Gly Ser Ser Ser Gly Gln Tyr
35 40 45
Tyr Asn Gly Gly Asp Asn His Glu Tyr Asp Gly Val Gly Ala Ala Glu
50 55 60
Glu Met Met Ile Met Glu Glu Glu Glu Gln Asn Glu Cys Asn Ser Val
65 70 75 80
Gly Ser Phe Tyr Val Lys Val Asn Met Glu Gly Val Pro Ile Gly Arg
85 90 95
Lys Ile Asp Leu Leu Ser Leu Asn Gly Tyr His Asp Leu Ile Thr Thr
100 105 110
Leu Asp Tyr Met Phe Asn Ala Ser Ile Leu Trp Ala Glu Glu Glu Asp
115 120 125
Met Cys Ser Glu Lys Ser His Val Leu Thr Tyr Ala Asp Lys Glu Gly
130 135 140
Asp Trp Met Met Val Gly Asp Val Pro Trp Glu Met Phe Leu Ser Ser
145 150 155 160
Val Arg Arg Leu Lys Ile Ser Arg Ala Tyr His Tyr
165 170
<210>11
<211>186
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5g43700
<400>11
Met Glu Lys Val Asp Val Tyr Asp Glu Leu Val Asn Leu Lys Ala Thr
1 5 10 15
Glu Leu Arg Leu Gly Leu Pro Gly Thr Glu Glu Thr Val Ser Cys Gly
20 25 30
Lys Ser Asn Lys Arg Val Leu Pro Glu Ala Thr Glu Lys Glu Ile Glu
35 40 45
Ser Thr Gly Lys Thr Glu Thr Ala Ser Pro Pro Lys Ala Gln Ile Val
50 55 60
Gly Trp Pro Pro Val Arg Ser Tyr Arg Lys Asn Asn Val Gln Thr Lys
65 70 75 80
Lys Ser Glu Ser Glu Gly Gln Gly Asn Tyr Val Lys Val Ser Met Asp
85 90 95
Gly Ala Pro Tyr Leu Arg Lys Ile Asp Leu Thr Met Tyr Lys Gln Tyr
100 105 110
Pro Glu Leu Met Lys Ser Leu Glu Asn Met Phe Lys Phe Ser Val Gly
115 120 125
Glu Tyr Phe Glu Arg Glu Gly Tyr Lys Gly Ser Asp Phe Val Pro Thr
130 135 140
Tyr Glu Asp Lys Asp Gly Asp Trp Met Leu Val Gly Asp Val Pro Trp
145 150 155 160
Glu Met Phe Val Ser Ser Cys Lys Arg Leu Arg Ile Met Lys Gly Ser
165 170 175
Glu Val Lys Gly Leu Gly Cys Gly Gly Leu
180 185
<210>12
<211>788
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5g60450
<400>12
Met Glu Phe Asp Leu Asn Thr Glu Ile Ala Glu Val Glu Glu Glu Glu
1 5 10 15
Asn Asp Asp Val Gly Val Gly Val Gly Gly Gly Thr Arg Ile Asp Lys
20 25 30
Gly Arg Leu Gly Ile Ser Pro Ser Ser Ser Ser Ser Cys Ser Ser Gly
35 40 45
Ser Ser Ser Ser Ser Ser Ser Thr Gly Ser Ala Ser Ser Ile Tyr Ser
50 55 60
Glu Leu Trp His Ala Cys Ala Gly Pro Leu Thr Cys Leu Pro Lys Lys
65 70 75 80
Gly Asn Val Val Val Tyr Phe Pro Gln Gly His Leu Glu Gln Asp Ala
85 90 95
Met Val Ser Tyr Ser Ser Pro Leu Glu Ile Pro Lys Phe Asp Leu Asn
100 105 110
Pro Gln Ile Val Cys Arg Val Val Asn Val Gln Leu Leu Ala Asn Lys
115 120 125
Asp Thr Asp Glu Val Tyr Thr Gln Val Thr Leu Leu Pro Leu Gln Glu
130 135 140
Phe Ser Met Leu Asn Gly Glu Gly Lys Glu Val Lys Glu Leu Gly Gly
145 150 155 160
Glu Glu Glu Arg Asn Gly Ser Ser Ser Val Lys Arg Thr Pro His Met
165 170 175
Phe Cys Lys Thr Leu Thr Ala Ser Asp Thr Ser Thr His Gly Gly Phe
180 185 190
Ser Val Pro Arg Arg Ala Ala Glu Asp Cys Phe Ala Pro Leu Asp Tyr
195 200 205
Lys Gln Gln Arg Pro Ser Gln Glu Leu Ile Ala Lys Asp Leu His Gly
210 215 220
Val Glu Trp Lys Phe Arg His Ile Tyr Arg Gly Gln Pro Arg Arg His
225 230 235 240
Leu Leu Thr Thr Gly Trp Ser Ile Phe Val Ser Gln Lys Asn Leu Val
245 250 255
Ser Gly Asp Ala Val Leu Phe Leu Arg Asp Glu Gly Gly Glu Leu Arg
260 265 270
Leu Gly Ile Arg Arg Ala Ala Arg Pro Arg Asn Gly Leu Pro Asp Ser
275 280 285
Ile Ile Glu Lys Asn Ser Cys Ser Asn Ile Leu Ser Leu Val Ala Asn
290 295 300
Ala Val Ser Thr Lys Ser Met Phe His Val Phe Tyr Ser Pro Arg Ala
305 310 315 320
Thr His Ala Glu Phe Val Ile Pro Tyr Glu Lys Tyr Ile Thr Ser Ile
325 330 335
Arg Ser Pro Val Cys Ile Gly Thr Arg Phe Arg Met Arg Phe Glu Met
340 345 350
Asp Asp Ser Pro Glu Arg Arg Cys Ala Gly Val Val Thr Gly Val Cys
355 360 365
Asp Leu Asp Pro Tyr Arg Trp Pro Asn Ser Lys Trp Arg Cys Leu Leu
370 375 380
Val Arg Trp Asp Glu Ser Phe Val Ser Asp His Gln Glu Arg Val Ser
385 390 395 400
Pro Trp Glu Ile Asp Pro Ser Val Ser Leu Pro His Leu Ser Ile Gln
405 410 415
Ser Ser Pro Arg Pro Lys Arg Pro Trp Ala Gly Leu Leu Asp Thr Thr
420 425 430
Pro Pro Gly Asn Pro Ile Thr Lys Arg Gly Gly Phe Leu Asp Phe Glu
435 440 445
Glu Ser Val Arg Pro Ser Lys Val Leu Gln Gly Gln Glu Asn Ile Gly
450 455 460
Ser Ala Ser Pro Ser Gln Gly Phe Asp Val Met Asn Arg Arg Ile Leu
465 470 475 480
Asp Phe Ala Met Gln Ser His Ala Asn Pro Val Leu Val Ser Ser Arg
485 490 495
Val Lys Asp Arg Phe Gly Glu Phe Val Asp Ala Thr Gly Val Asn Pro
500 505 510
Ala Cys Ser Gly Val Met Asp Leu Asp Arg Phe Pro Arg Val Leu Gln
515 520 525
Gly Gln Glu Ile Cys Ser Leu Lys Ser Phe Pro Gln Phe Ala Gly Phe
530 535 540
Ser Pro Ala Ala Ala Pro Asn Pro Phe Ala Tyr Gln Ala Asn Lys Ser
545 550 555 560
Ser Tyr Tyr Pro Leu Ala Leu His Gly Ile Arg Ser Thr His Val Pro
565 570 575
Tyr Gln Asn Pro Tyr Asn Ala Gly Asn Gln Ser Ser Gly Pro Pro Ser
580 585 590
Arg Ala Ile Asn Phe Gly Glu Glu Thr Arg Lys Phe Asp Ala Gln Asn
595 600 605
Glu Gly Gly Leu Pro Asn Asn Val Thr Ala Asp Leu Pro Phe Lys Ile
610 615 620
Asp Met Met Gly Lys Gln Lys Gly Ser Glu Leu Asn Met Asn Ala Ser
625 630 635 640
Ser Gly Cys Lys Leu Phe Gly Phe Ser Leu Pro Val Glu Thr Pro Ala
645 650 655
Ser Lys Pro Gln Ser Ser Ser Lys Arg Ile Cys Thr Lys Val His Lys
660 665 670
Gln Gly Ser Gln Val Gly Arg Ala Ile Asp Leu Ser Arg Leu Asn Gly
675 680 685
Tyr Asp Asp Leu Leu Met Glu Leu Glu Arg Leu Phe Asn Met Glu Gly
690 695 700
Leu Leu Arg Asp Pro Glu Lys Gly Trp Arg Ile Leu Tyr Thr Asp Ser
705 710 715 720
Glu Asn Asp Met Met Val Val Gly Asp Asp Pro Trp His Asp Phe Cys
725 730 735
Asn Val Val Trp Lys Ile His Leu Tyr Thr Lys Glu Glu Val Glu Asn
740 745 750
Ala Asn Asp Asp Asn Lys Ser Cys Leu Glu Gln Ala Ala Leu Met Met
755 760 765
Glu Ala Ser Lys Ser Ser Ser Val Ser Gln Pro Asp Ser Ser Pro Thr
770 775 780
Ile Thr Arg Val
785
<210>13
<211>294
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At4g00940
<400>13
Met Asp His His Gln Tyr His His His Asp Gln Tyr Gln His Gln Met
1 5 10 15
Met Thr Ser Thr Asn Asn Asn Ser Tyr Asn Thr Ile Val Thr Thr Gln
20 25 30
Pro Pro Pro Thr Thr Thr Thr Met Asp Ser Thr Thr Ala Thr Thr Met
35 40 45
Ile Met Asp Asp Glu Lys Lys Leu Met Thr Thr Met Ser Thr Arg Pro
50 55 60
Gln Glu Pro Arg Asn Cys Pro Arg Cys Asn Ser Ser Asn Thr Lys Phe
65 70 75 80
Cys Tyr Tyr Asn Asn Tyr Ser Leu Ala Gln Pro Arg Tyr Leu Cys Lys
85 90 95
Ser Cys Arg Arg Tyr Trp Thr Glu Gly Gly Ser Leu Arg Asn Val Pro
100 105 110
Val Gly Gly Gly Ser Arg Lys Asn Lys Lys Leu Pro Phe Pro Asn Ser
115 120 125
Ser Thr Ser Ser Ser Thr Lys Asn Leu Pro Asp Leu Asn Pro Pro Phe
130 135 140
Val Phe Thr Ser Ser Ala Ser Ser Ser Asn Pro Ser Lys Thr His Gln
145 150 155 160
Asn Asn Asn Asp Leu Ser Leu Ser Phe Ser Ser Pro Met Gln Asp Lys
165 170 175
Arg Ala Gln Gly His Tyr Gly His Phe Ser Glu Gln Val Val Thr Gly
180 185 190
Gly Gln Asn Cys Leu Phe Gln Ala Pro Met Gly Met Ile Gln Phe Arg
195 200 205
Gln Glu Tyr Asp His Glu His Pro Lys Lys Asn Leu Gly Phe Ser Leu
210 215 220
Asp Arg Asn Glu Glu Glu Ile Gly Asn His Asp Asn Phe Val Val Asn
225 230 235 240
Glu Glu Gly Ser Lys Met Met Tyr Pro Tyr Gly Asp His Glu Asp Arg
245 250 255
Gln Gln His His His Val Arg His Asp Asp Gly Asn Lys Lys Arg Glu
260 265 270
Gly Gly Ser Ser Asn Glu Leu Trp Ser Gly Ile Ile Leu Gly Gly Asp
275 280 285
Ser Gly Gly Pro Thr Trp
290
<210>14
<211>444
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At1g27050
<400>14
Met Asp Glu Glu Asp Val Cys Glu Ser Tyr Met Met Arg Glu Ile Thr
1 5 10 15
Lys Lys Arg Lys Leu Thr Pro Ile Gln Leu Arg Leu Leu Glu Glu Ser
20 25 30
Phe Glu Glu Glu Lys Arg Leu Glu Pro Asp Arg Lys Leu Trp Leu Ala
35 40 45
Glu Lys Leu Gly Leu Gln Pro Ser Gln Val Ala Val Trp Phe Gln Asn
50 55 60
Arg Arg Ala Arg Tyr Lys Thr Lys Gln Leu Glu His Asp Cys Asp Ser
65 70 75 80
Leu Lys Ala Ser Tyr Ala Lys Leu Lys Thr Asp Trp Asp Ile Leu Phe
85 90 95
Val Gln Asn Gln Thr Leu Lys Ser Lys Val Gln Phe Leu Asn Arg Leu
100 105 110
Thr Ser His Tyr Phe Gln Glu Ser Val Gln Asn Phe Asp Asp Thr Phe
115 120 125
Lys Gln Val Asp Leu Leu Lys Glu Lys Leu Lys Met Gln Glu Asn Leu
130 135 140
Glu Thr Gln Ser Ile Glu Arg Lys Arg Leu Gly Glu Glu Gly Ser Ser
145 150 155 160
Val Lys Ser Asp Asn Thr Gln Tyr Ser Glu Glu Glu Gly Leu Glu Asn
165 170 175
Gln Tyr Ser Phe Pro Glu Leu Ala Val Leu Gly Phe Tyr Tyr Asp Pro
180 185 190
Thr Leu Thr Ala Ser Asn Leu Arg Gln Glu Pro Leu Lys Val Thr Cys
195 200 205
Ala Asp Gln Met Thr Gln Ile Gln Ile Ser Asp Val Thr Glu Pro Ala
210 215 220
Ser Ser Ala His Lys Lys Ile Glu Val Thr Gln Arg Ser Ser Ser Met
225 230 235 240
Ser Arg Lys Arg Asp Lys Pro Tyr Thr Asn Arg His Thr Pro Ala Arg
245 250 255
Ile Ser Lys Arg Arg Arg Pro Trp Ala Pro Ser Ser Ser Glu His Asp
260 265 270
Glu Ile Ile Asp Lys Pro Ile Thr Lys Pro Pro Pro Pro Pro Ala Leu
275 280 285
Val Val Met Gly Leu Pro Ala Asn Cys Ser Val Leu Glu Leu Lys Ser
290 295 300
Arg Phe Glu Ile Tyr Gly Ser Ile Ser Arg Ile Arg Ile His Lys Asp
305 310 315 320
Gly Ile Gly Ser Val Ser Tyr Arg Thr Ala Glu Ser Ala Glu Ala Ala
325 330 335
Ile Ala Gly Ser His Glu Pro Ser Phe Gly Ile Ser Ile Asp Ser Lys
340 345 350
Lys Leu Glu Val Val Trp Ala Thr Asp Pro Leu Val Lys Trp Lys Glu
355 360 365
Gly Val Thr Ala Gly Glu Gly Lys Glu Arg Thr Ser Ser Phe Ser Ser
370 375 380
Lys Leu Leu Arg Pro Val Met Pro Leu Arg Lys His Gly Arg Ser Ser
385 390 395 400
Arg Leu Ala Ser Ala Ile Val Asn Pro Arg Ser Asp Asn Thr Lys Gly
405 410 415
Ile Ser Gly Asp Gly Gly Ile Ser Ser Pro Ala Thr Thr Ser Glu Val
420 425 430
Lys Gln Arg Asn Ile Val Thr Tyr Asp Asp Ile Val
435 440
<210>15
<211>275
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At2g01430
<400>15
Met Ile Lys Leu Leu Phe Thr Tyr Ile Cys Thr Tyr Thr Tyr Lys Leu
1 5 10 15
Tyr Ala Leu Tyr His Met Asp Tyr Ala Cys Val Cys Met Tyr Lys Tyr
20 25 30
Lys Gly Ile Val Thr Leu Gln Val Cys Leu Phe Tyr Ile Lys Leu Arg
35 40 45
Val Phe Leu Ser Asn Phe Thr Phe Ser Ser Ser Ile Leu Ala Leu Lys
50 55 60
Asn Pro Asn Asn Ser Leu Ile Lys Ile Met Ala Ile Leu Pro Glu Asn
65 70 75 80
Ser Ser Asn Leu Asp Leu Thr Ile Ser Val Pro Gly Phe Ser Ser Ser
85 90 95
Pro Leu Ser Asp Glu Gly Ser Gly Gly Gly Arg Asp Gln Leu Arg Leu
100 105 110
Asp Met Asn Arg Leu Pro Ser Ser Glu Asp Gly Asp Asp Glu Glu Phe
115 120 125
Ser His Asp Asp Gly Ser Ala Pro Pro Arg Lys Lys Leu Arg Leu Thr
130 135 140
Arg Glu Gln Ser Arg Leu Leu Glu Asp Ser Phe Arg Gln Asn His Thr
145 150 155 160
Leu Asn Pro Lys Gln Lys Glu Val Leu Ala Lys His Leu Met Leu Arg
165 170 175
Pro Arg Gln Ile Glu Val Trp Phe Gln Asn Arg Arg Ala Arg Ser Lys
180 185 190
Leu Lys Gln Thr Glu Met Glu Cys Glu Tyr Leu Lys Arg Trp Phe Gly
195 200 205
Ser Leu Thr Glu Glu Asn His Arg Leu His Arg Glu Val Glu Glu Leu
210 215 220
Arg Ala Met Lys Val Gly Pro Thr Thr Val Asn Ser Ala Ser Ser Leu
225 230 235 240
Thr Met Cys Pro Arg Cys Glu Arg Val Thr Pro Ala Ala Ser Pro Ser
245 250 255
Arg Ala Val Val Pro Val Pro Ala Lys Lys Thr Phe Pro Pro Gln Glu
260 265 270
Arg Asp Arg
275
<210>16
<211>436
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At1g14350
<400>16
Met Glu Asp Thr Lys Lys Lys Lys Lys Lys Asn Ile Asn Asn Asn Gln
1 5 10 15
Asp Ser Lys Lys Lys Glu Arg His Ile Val Thr Trp Ser Gln Glu Glu
20 25 30
Asp Val Ile Leu Arg Glu Gln Ile Thr Leu His Gly Thr Glu Asn Trp
35 40 45
Ala Ile Ile Ala Ser Lys Phe Lys Asp Lys Ser Thr Arg Gln Cys Arg
50 55 60
Arg Arg Trp Tyr Thr Tyr Leu Asn Ser Asp Phe Lys Arg Gly Gly Trp
65 70 75 80
Ser Pro Glu Glu Asp Met Leu Leu Cys Glu Ala Gln Arg Val Phe Gly
85 90 95
Asn Arg Trp Thr Glu Ile Ala Lys Val Val Ser Gly Arg Thr Asp Asn
100 105 110
Ala Val Lys Asn Arg Phe Thr Thr Leu Cys Lys Lys Arg Ala Lys His
115 120 125
Glu Ala Met Thr Lys Asp Ser Asn Ser Asn Thr Lys Arg Met Leu Phe
130 135 140
Leu Asp Gly Ile Ser Thr Pro Arg Lys Ser Glu Asn Glu Thr Pro Ile
145 150 155 160
Ala Lys Lys Leu Lys Arg Ser His Ile Leu Asp Leu Thr Glu Ile Ser
165 170 175
Asn Tyr Gly Arg Ala Glu Ala Cys Val Asn Gln Gln Ile Arg Ser Pro
180 185 190
Phe Ser Val Leu Ala Arg Asn Ala Thr Gly Ile Asp Ser Leu Glu Glu
l95 200 205
Gln Asn Gln Thr Ser Asn Val Asn Glu Ser Asp Gly Glu Gly Met Phe
210 215 220
Leu Lys Lys Asp Asp Pro Lys Val Thr Ala Leu Met Gln Gln Ala Glu
225 230 235 240
Leu Leu Ser Ser Leu Ala Gln Lys Val Asn Ala Asp Asn Thr Glu Gln
245 250 255
Ser Met Glu Asn Ala Trp Lys Val Leu Gln Asp Phe Leu Asn Lys Gly
260 265 270
Lys Glu Asn Asp Leu Phe Arg Tyr Gly Ile Pro Asp Ile Asp Phe Lys
275 280 285
Ile Glu Glu Phe Lys Asp Leu Ile Glu Asp Leu Arg Ser Gly Tyr Glu
290 295 300
Asp Asn Gln Leu Ser Trp Arg Gln Pro Asp Leu His Asp Ser Pro Ala
305 310 315 320
Ser Ser Glu Tyr Ser Ser Gly Ser Thr Ile Met Val Asp Gln Ser Gly
325 330 335
Asp Lys Thr Gln Pro Phe Ser Ala Asp Thr Gln Thr Glu His Lys Gln
340 345 350
Val Gly Glu Glu Leu Leu Val Pro Lys Asn Pro Asp Glu Asn Met Pro
355 360 365
Ile Ser Gly Glu Glu Lys Phe Ser Ser Pro Ile Gln Val Thr Pro Leu
370 375 380
Phe Arg Ser Leu Ala Asp Gly Ile Pro Ser Pro Gln Phe Ser Glu Ser
385 390 395 400
Glu Arg Ser Phe Leu Leu Lys Thr Leu Gly Ile Glu Ser Ser Ser Pro
405 410 415
Cys Pro Ser Ala Asn Pro Ser Lys Pro Pro Pro Cys Lys Arg Val Leu
420 425 430
Leu His Ser Leu
435
<210>17
<211>352
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At1g18570
<400>17
Met Val Arg Thr Pro Cys Cys Lys Ala Glu Leu Gly Leu Lys Lys Gly
1 5 10 15
Ala Trp Thr Pro Glu Glu Asp Gln Lys Leu Leu Ser Tyr Leu Asn Arg
20 25 30
His Gly Glu Gly Gly Trp Arg Thr Leu Pro Glu Lys Ala Gly Leu Lys
35 40 45
Arg Cys Gly Lys Ser Cys Arg Leu Arg Trp Ala Asn Tyr Leu Arg Pro
50 55 60
Asp Ile Lys Arg Gly Glu Phe Thr Glu Asp Glu Glu Arg Ser Ile Ile
65 70 75 80
Ser Leu His Ala Leu His Gly Asn Lys Trp Ser Ala Ile Ala Arg Gly
85 90 95
Leu Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn Tyr Trp Asn Thr His
100 105 110
Ile Lys Lys Arg Leu Ile Lys Lys Gly Ile Asp Pro Val Thr His Lys
115 120 125
Gly Ile Thr Ser Gly Thr Asp Lys Ser Glu Asn Leu Pro Glu Lys Gln
130 135 140
Asn Val Asn Leu Thr Thr Ser Asp His Asp Leu Asp Asn Asp Lys Ala
145 150 155 160
Lys Lys Asn Asn Lys Asn Phe Gly Leu Ser Ser Ala Ser Phe Leu Asn
165 170 175
Lys Val Ala Asn Arg Phe Gly Lys Arg Ile Asn Gln Ser Val Leu Ser
180 185 190
Glu Ile Ile Gly Ser Gly Gly Pro Leu Ala Ser Thr Ser His Thr Thr
195 200 205
Asn Thr Thr Thr Thr Ser Val Ser Val Asp Ser Glu Ser Val Lys Ser
210 215 220
Thr Ser Ser Ser Phe Ala Pro Thr Ser Asn Leu Leu Cys His Gly Thr
225 230 235 240
Val Ala Thr Thr Pro Val Ser Ser Asn Phe Asp Val Asp Gly Asn Val
245 250 255
Ash Leu Thr Cys Ser Ser Ser Thr Phe Ser Asp Ser Ser Val Asn Asn
260 265 270
Pro Leu Met Tyr Cys Asp Asn Phe Val Gly Asn Asn Asn Val Asp Asp
275 280 285
Glu Asp Thr Ile Gly Phe Ser Thr Phe Leu Asn Asp Glu Asp Phe Met
290 295 300
Met Leu Glu Glu Ser Cys Val Glu Asn Thr Ala Phe Met Lys Glu Leu
305 310 315 320
Thr Arg Phe Leu His Glu Asp Glu Asn Asp Val Val Asp Val Thr Pro
325 330 335
Val Tyr Glu Arg Gln Asp Leu Phe Asp Glu Ile Asp Asn Tyr Phe Gly
340 345 350
<210>18
<211>337
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At2g47260
<400>18
Met Glu Phe Thr Asp Phe Ser Lys Thr Ser Phe Tyr Tyr Pro Ser Ser
1 5 10 15
Gln Ser Val Trp Asp Phe Gly Asp Leu Ala Ala Ala Glu Arg His Ser
20 25 30
Leu Gly Phe Met Glu Leu Leu Ser Ser Gln Gln His Gln Asp Phe Ala
35 40 45
Thr Val Ser Pro His Ser Phe Leu Leu Gln Thr Ser Gln Pro Gln Thr
50 55 60
Gln Thr Gln Pro Ser Ala Lys Leu Ser Ser Ser Ile Ile Gln Ala Pro
65 70 75 80
Pro Ser Glu Gln Leu Val Thr Ser Lys Val Glu Ser Leu Cys Ser Asp
85 90 95
His Leu Leu Ile Asn Pro Pro Ala Thr Pro Asn Ser Ser Ser Ile Ser
100 105 110
Ser Ala Ser Ser Glu Ala Leu Asn Glu Glu Lys Pro Lys Thr Glu Asp
115 120 125
Asn Glu Glu Glu Gly Gly Glu Asp Gln Gln Glu Lys Ser His Thr Lys
130 135 140
Lys Gln Leu Lys Ala Lys Lys Asn Asn Gln Lys Arg Gln Arg Glu Ala
145 150 155 160
Arg Val Ala Phe Met Thr Lys Ser Glu Val Asp His Leu Glu Asp Gly
165 170 175
Tyr Arg Trp Arg Lys Tyr Gly Gln Lys Ala Val Lys Asn Ser Pro Phe
180 185 190
Pro Arg Ser Tyr Tyr Arg Cys Thr Thr Ala Ser Cys Asn Val Lys Lys
195 200 205
Arg Val Glu Arg Ser Phe Arg Asp Pro Ser Thr Val Val Thr Thr Tyr
210 215 220
Glu Gly Gln His Thr His Ile Ser Pro Leu Thr Ser Arg Pro Ile Ser
225 230 235 240
Thr Gly Gly Phe Phe Gly Ser Ser Gly Ala Ala Ser Ser Leu Gly Asn
245 250 255
Gly Cys Phe Gly Phe Pro Ile Asp Gly Ser Thr Leu Ile Ser Pro Gln
260 265 270
Phe Gln Gln Leu Val Gln Tyr His His Gln Gln Gln Gln Gln Glu Leu
275 280 285
Met Ser Cys Phe Gly Gly Val Asn Glu Tyr Leu Asn Ser His Ala Asn
290 295 300
Glu Tyr Gly Asp Asp Asn Arg Val Lys Lys Ser Arg Val Leu Val Lys
305 310 315 320
Asp Asn Gly Leu Leu Gln Asp Val Val Pro Ser His Met Leu Lys Glu
325 330 335
Glu
<210>19
<211>120
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5g26930
<400>19
Met Asp Pro Arg Lys Leu Leu Ser Cys Ser Ser Ser Tyr Val Ser Val
1 5 10 15
Arg Met Lys Glu Glu Lys Gly Thr Ile Arg Cys Cys Ser Glu Cys Lys
20 25 30
Thr Thr Lys Thr Pro Met Trp Arg Gly Gly Pro Thr Gly Pro Lys Ser
35 40 45
Leu Cys Asn Ala Cys Gly Ile Arg His Arg Lys Gln Arg Arg Ser Glu
50 55 60
Leu Leu Gly Ile His Ile Ile Arg Ser His Lys Ser Leu Ala Ser Lys
65 70 75 80
Lys Ile Asn Leu Leu Ser Ser Ser His Gly Gly Val Ala Val Lys Lys
85 90 95
Arg Arg Ser Leu Lys Glu Glu Glu Gln Ala Ala Leu Cys Leu Leu Leu
100 105 110
Leu Ser Cys Ser Ser Val Leu Ala
115 120
<210>20
<211>1090
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5G63950
<400>20
Met Ala Glu Asn Thr Ala Ser His Arg Arg Lys Pro Arg Ser Leu Asn
1 5 10 15
Asp Arg His Tyr Ser Ile Leu Gln Asp Leu Ser Ala Pro Pro Arg Gln
20 25 30
Pro Pro Ser Ser Ser His Gly Glu Asp Glu Glu Thr Lys Lys Ser Met
35 40 45
Ile Lys Leu Ala Gly Arg Arg Arg Leu Cys Lys Ala Leu Pro Lys Glu
50 55 60
Asp Glu Ala Asp Gly Tyr Asp Asp Pro Asp Leu Val Asp Phe Tyr Ser
65 70 75 80
Pro Val Lys Gly Glu Thr Ser Leu Asp Ser Ala Gly Ile Gly Asn Lys
85 90 95
Phe Thr Ser Trp Asp Glu Ser Lys Glu Ala Asn Thr Glu Leu Ala Gly
100 105 110
Glu Pro Asn Phe Ser Ile Ile Thr Asp Phe Cys Ser Pro Ser Pro Gln
115 120 125
Leu Lys Gln Lys Glu Glu Met Gln Gly Asp Gly Gly Arg Asn Glu Ile
130 135 140
Met Gly Ile Leu Asp Asp Leu Thr Ser Lys Leu Gly Thr Met Ser Ile
145 150 155 160
Gln Lys Lys Lys Asp Ser Gln Ser Asn Asp Phe Asp Ala Cys Gly Val
165 170 175
Lys Ser Gln Val Asp Lys Phe Asp Phe Glu Asp Ala Lys Ser Ser Phe
180 185 190
Ser Leu Leu Ser Asp Leu Ser Lys Ser Ser Pro Asp Val Val Thr Thr
195 200 205
Tyr Asn Ala Gly Val Asn Ser Ile Lys Asp Lys Gln Gly Lys Ser Gly
210 215 220
Phe Ala Ile Arg Glu Glu Gln Thr Ser Lys Glu Phe Ser Arg Glu Trp
225 230 235 240
Glu Glu Arg Ile Ser Asn Val Gly Lys Gln Asn Ser Tyr Ser Gly Arg
245 250 255
His Phe Asp Asp Asn Ser Glu Asp Asn Arg Gln Gly Tyr Asn Leu Asp
260 265 270
Arg Gly Lys Ser Gln Cys Lys Glu Val Asp Gln Ser Met Lys Thr Thr
275 280 285
Arg His Ile Glu Val Ser Glu Lys Ile Arg Thr Val Gly Arg Ser Asn
290 295 300
Ala Ala Lys Leu Arg Asp Leu Asp Glu Asp Asp Asp Asp Asp Asp Cys
305 310 315 320
Leu Ile Leu Ser Gly Lys Lys Ala Ala Glu Met Lys Ile Asn Lys Pro
325 330 335
Ala Arg Ser Tyr Asn Ala Lys Arg His Gly Tyr Asp Glu Arg Ser Leu
340 345 350
Glu Asp Glu Gly Ser Ile Thr Leu Thr Gly Leu Asn Leu Ser Tyr Thr
355 360 365
Leu Pro Gly Lys Ile Ala Thr Met Leu Tyr Pro His Gln Arg Glu Gly
370 375 380
Leu Asn Trp Leu Trp Ser Leu His Thr Gln Gly Lys Gly Gly Ile Leu
385 390 395 400
Gly Asp Asp Met Gly Leu Gly Lys Thr Met Gln Ile Cys Ser Phe Leu
405 410 415
Ala Gly Leu Phe His Ser Lys Leu Ile Lys Arg Ala Leu Val Val Ala
420 425 430
Pro Lys Thr Leu Leu Pro His Trp Met Lys Glu Leu Ala Thr Val Gly
435 440 445
Leu Ser Gln Met Thr Arg Glu Tyr Tyr Gly Thr Ser Thr Lys Ala Arg
450 455 460
Glu Tyr Asp Leu His His Ile Leu Gln Gly Lys Gly Ile Leu Leu Thr
465 470 475 480
Thr Tyr Asp Ile Val Arg Asn Asn Thr Lys Ala Leu Gln Gly Asp Asp
485 490 495
His Tyr Thr Asp Glu Asp Asp Glu Asp Gly Asn Lys Trp Asp Tyr Met
500 505 510
Ile Leu Asp Glu Gly His Leu Ile Lys Asn Pro Asn Thr Gln Arg Ala
515 520 525
Lys Ser Leu Leu Glu Ile Pro Ser Ser His Arg Ile Ile Ile Ser Gly
530 535 540
Thr Pro Ile Gln Asn Asn Leu Lys Glu Leu Trp Ala Leu Phe Asn Phe
545 550 555 560
Ser Cys Pro Gly Leu Leu Gly Asp Lys Asn Trp Phe Lys Gln Asn Tyr
565 570 575
Glu His Tyr Ile Leu Arg Gly Thr Asp Lys Asn Ala Thr Asp Arg Glu
580 585 590
Gln Arg Ile Gly Ser Thr Val Ala Lys Asn Leu Arg Glu His Ile Gln
595 600 605
Pro Phe Phe Leu Arg Arg Leu Lys Ser Glu Val Phe Gly Asp Asp Gly
610 615 620
Ala Thr Ser Lys Leu Ser Lys Lys Asp Glu Ile Val Val Trp Leu Arg
625 630 635 640
Leu Thr Ala Cys Gln Arg Gln Leu Tyr Glu Ala Phe Leu Asn Ser Glu
645 650 655
Ile Val Leu Ser Ala Phe Asp Gly Ser Pro Leu Ala Ala Leu Thr Ile
660 665 670
Leu Lys Lys Ile Cys Asp His Pro Leu Leu Leu Thr Lys Arg Ala Ala
675 680 685
Glu Asp Val Leu Glu Gly Met Asp Ser Thr Leu Thr Gln Glu Glu Ala
690 695 700
Gly Val Ala Glu Arg Leu Ala Met His Ile Ala Asp Asn Val Asp Thr
705 710 715 720
Asp Asp Phe Gln Thr Lys Asn Asp Ser Ile Ser Cys Lys Leu Ser Phe
725 730 735
Ile Met Ser Leu Leu Glu Asn Leu Ile Pro Glu Gly His Arg Val Leu
740 745 750
Ile Phe Ser Gln Thr Arg Lys Met Leu Asn Leu Ile Gln Asp Ser Leu
755 760 765
Thr Ser Asn Gly Tyr Ser Phe Leu Arg Ile Asp Gly Thr Thr Lys Ala
770 775 780
Pro Asp Arg Leu Lys Thr Val Glu Glu Phe Gln Glu Gly His Val Ala
785 790 795 800
Pro Ile Phe Leu Leu Thr Ser Gln Val Gly Gly Leu Gly Leu Thr Leu
805 810 815
Thr Lys Ala Asp Arg Val Ile Val Val Asp Pro Ala Trp Asn Pro Ser
820 825 830
Thr Asp Asn Gln Ser Val Asp Arg Ala Tyr Arg Ile Gly Gln Thr Lys
835 840 845
Asp Val Ile Val Tyr Arg Leu Met Thr Ser Ala Thr Val Glu Glu Lys
850 855 860
Ile Tyr Arg Lys Gln Val Tyr Lys Gly Gly Leu Phe Lys Thr Ala Thr
865 870 875 880
Glu His Lys Glu Gln Ile Arg Tyr Phe Ser Gln Gln Asp Leu Arg Glu
885 890 895
Leu Phe Ser Leu Pro Lys Gly Gly Phe Asp Val Ser Pro Thr Gln Gln
900 905 910
Gln Leu Tyr Glu Glu His Tyr Asn Gln Ile Lys Leu Asp Glu Lys Leu
915 920 925
Glu Ser His Val Lys Phe Leu Glu Thr Leu Gly Ile Ala Gly Val Ser
930 935 940
His His Ser Leu Leu Phe Ser Lys Thr Ala Pro Ile Gln Ala Ile Gln
945 950 955 960
Lys Asp Glu Glu Glu Gln Ile Arg Arg Glu Thr Ala Leu Leu Leu Gly
965 970 975
Arg Ala Ser Ala Ser Ile Ser Gln Asp Thr Val Ile Asn Gly Ala Asp
980 985 990
Tyr Ala Phe Lys Pro Lys Asp Val Asn Leu Asp Lys Arg Ile Asn Ile
995 1000 1005
Ser Pro Val Asp Asp Lys Glu Leu Ser Glu Ser Val Ile Lys Ala
1010 1015 1020
Arg Leu Asn Arg Leu Thr Met Leu Leu Gln Asn Lys Gly Thr Val
1025 1030 1035
Ser Arg Leu Pro Asp Gly Gly Ala Lys Ile Gln Lys Gln Ile Ala
1040 1045 1050
Glu Leu Thr Arg Glu Leu Lys Asp Met Lys Ala Ala Glu Arg Ile
1055 1060 1065
Asn Met Pro Gln Val Ile Asp Leu Glu Glu Asp Ile Ser Arg Lys
1070 1075 1080
Met Gln Lys Gly Leu Asn Leu
1085 1090
<210>21
<211>406
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5G47440
<400>21
Met Glu Gly Gly Phe Tyr Ser Asp Trp Asn Asp Ser Ser Ser Ser Leu
1 5 10 15
Phe Gly Ser Glu Asn Pro Glu His Glu Leu Glu Glu Gly Asn Val Arg
20 25 30
Ser Glu Glu Ile Val Ser Gln Ile Pro Gln Pro Gln Thr Pro Arg Glu
35 40 45
Pro Met Lys Phe Leu Ser Arg Ser Trp Ser Leu Ser Ala Ser Glu Ile
50 55 60
Ser Lys Ala Leu Ala Gln Lys Gln Arg Gln Gln Arg Asp Leu Phe Ser
65 70 75 80
Val Ser Gln Asn Ser Pro Arg Gly Phe Phe Gln Asp Val Ala Ala Asp
85 90 95
Pro Leu Met Ala Glu Asn Ile Met Asn Ser Ala Gly Thr Arg Arg Ser
100 105 110
Gly Arg Leu Ser Lys Trp Phe His His Lys Gln His Thr Asn Pro Ser
115 120 125
Thr Met Arg Ile Pro Arg Lys Lys Asp Lys Ala Arg Val Gln Lys Ala
130 135 140
His Val His Ser Ala Val Ser Ile Ala Ala Leu Ala Ala Gly Leu Ala
145 150 155 160
Ser Val Thr Ser Glu Glu Ser Cys Ser Lys Glu Ser Cys Ser Met Met
165 170 175
Ala Leu Ala Leu Ala Ser Ala Thr Glu Leu Leu Ala Ser His Cys Ile
180 185 190
Asp Met Ala Glu Gln Ala Gly Ala Asp His Thr Cys Val Ala Ser Thr
195 200 205
Val Arg Ser Ser Val Asp Ile His Ser Pro Gly Asp Leu Met Thr Leu
210 215 220
Thr Ala Ala Ala Ala Thr Ala Leu Arg Gly Glu Ala Ala Leu Lys Val
225 2302 35 240
Arg Gln Pro Lys Glu Ser Arg Lys Asn Ala Thr Ile Thr Pro Cys Glu
245 250 255
Arg Ser Phe Ser Asp Ser His Trp Pro Gly Glu Asn Cys Gln Phe Arg
260 265 270
Leu Glu Glu Pro Asn Leu Pro Leu Glu Gly Glu Leu Val Gln Cys Ala
275 280 285
Arg Asn Gly Leu Gln Arg Asn Lys Arg Val Cys Val Tyr Ile Asn Lys
290 295 300
Lys Ser Gln Val Met Ile Lys Leu Lys Ser Lys His Val Gly Gly Ala
305 310 315 320
Phe Ser Lys Lys Ile Lys Cys Val Val Tyr Gly Val Cys Asp Glu Ile
325 330 335
Ser Ala Trp Pro Cys Arg Lys Glu Arg Glu Asn Ser Glu Glu Val Tyr
340 345 350
Phe Gly Leu Lys Thr Gly Gln Gly Leu Leu Glu Phe Lys Cys Lys Ser
355 360 365
Lys Ile Gln Lys Gln Arg Trp Val Ala Gly Ile Gln Ser Asn Leu Arg
370 375 380
Leu Val Ser Cys Leu Glu Ala Ala Lys Cys Ser Leu Glu Ser Leu Ser
385 390 395 400
Leu Ser Asn Arg Met Arg
405
<210>22
<211>250
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At1G69530
<400>22
Met Ala Leu Val Thr Phe Leu Phe Ile Ala Thr Leu Gly Ala Met Thr
1 5 10 15
Ser His Val Asn Gly Tyr Ala Gly Gly Gly Trp Val Asn Ala His Ala
20 25 30
Thr Phe Tyr Gly Gly Gly Asp Ala Ser Gly Thr Met Gly Gly Ala Cys
35 40 45
Gly Tyr Gly Asn Leu Tyr Ser Gln Gly Tyr Gly Thr Asn Thr Ala Ala
50 55 60
Leu Ser Thr Ala Leu Phe Asn Asn Gly Leu Ser Cys Gly Ala Cys Phe
65 70 75 80
Glu Ile Arg Cys Gln Asn Asp Gly Lys Trp Cys Leu Pro Gly Ser Ile
85 90 95
Val Val Thr Ala Thr Asn Phe Cys Pro Pro Asn Asn Ala Leu Pro Asn
100 105 110
Asn Ala Gly Gly Trp Cys Asn Pro Pro Gln Gln His Phe Asp Leu Ser
115 120 125
Gln Pro Val Phe Gln Arg Ile Ala Gln Tyr Arg Ala Gly Ile Val Pro
130 135 140
Val Ala Tyr Arg Arg Val Pro Cys Val Arg Arg Gly Gly Ile Arg Phe
145 150 155 160
Thr Ile Asn Gly His Ser Tyr Phe Asn Leu Val Leu Ile Thr Asn Val
165 170 175
Gly Gly Ala Gly Asp Val His Ser Ala Met Val Lys Gly Ser Arg Thr
180 185 190
Gly Trp Gln Ala Met Ser Arg Asn Trp Gly Gln Asn Trp Gln Ser Asn
195 200 205
Ser Tyr Leu Asn Gly Gln Ser Leu Ser Phe Lys Val Thr Thr Ser Asp
210 215 220
Gly Gln Thr Ile Val Ser Asn Asn Val Ala Asn Ala Gly Trp Ser Phe
225 230 235 240
Gly Gln Thr Phe Thr Gly Ala Gln Leu Arg
245 250
<210>23
<211>365
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At4G32460
<400>23
Met Lys Glu Met Gly Val Ile Val Leu Leu Leu Leu His Ser Phe Phe
1 5 10 15
Tyr Val Ala Phe Cys Phe Asn Asp Gly Leu Leu Pro Asn Gly Asp Phe
20 25 30
Glu Leu Gly Pro Arg His Ser Asp Met Lys Gly Thr Gln Val Ile Asn
35 40 45
Ile Thr Ala Ile Pro Asn Trp Glu Leu Ser Gly Phe Val Glu Tyr Ile
50 55 60
Pro Ser Gly His Lys Gln Gly Asp Met Ile Leu Val Val Pro Lys Gly
65 70 75 80
Ala Phe Ala Val Arg Leu Gly Asn Glu Ala Ser Ile Lys Gln Lys Ile
85 90 95
Ser Val Lys Lys Gly Ser Tyr Tyr Ser Ile Thr Phe Ser Ala Ala Arg
100 105 110
Thr Cys Ala Gln Asp Glu Arg Leu Asn Val Ser Val Ala Pro His His
115 120 125
Ala Val Met Pro Ile Gln Thr Val Tyr Ser Ser Ser Gly Trp Asp Leu
130 135 140
Tyr Ser Trp Ala Phe Lys Ala Gln Ser Asp Tyr Ala Asp Ile Val Ile
145 150 155 160
His Asn Pro Gly Val Glu Glu Asp Pro Ala Cys Gly Pro Leu Ile Asp
165 170 175
Gly Val Ala Met Arg Ala Leu Phe Pro Pro Arg Pro Thr Asn Lys Asn
180 185 190
Ile Leu Lys Asn Gly Gly Phe Glu Glu Gly Pro Trp Val Leu Pro Asn
195 200 205
Ile Ser Ser Gly Val Leu Ile Pro Pro Asn Ser Ile Asp Asp His Ser
210 215 220
Pro Leu Pro Gly Trp Met Val Glu Ser Leu Lys Ala Val Lys Tyr Ile
225 230 235 240
Asp Ser Asp His Phe Ser Val Pro Gln Gly Arg Arg Ala Val Glu Leu
245 250 255
Val Ala Gly Lys Glu Ser Ala Val Ala Gln Val Val Arg Thr Ile Pro
260 265 270
Gly Lys Thr Tyr Val Leu Ser Phe Ser Val Gly Asp Ala Ser Asn Ala
275 280 285
Cys Ala Gly Ser Met Ile Val Glu Ala Phe Ala Gly Lys Asp Thr Ile
290 295 300
Lys Val Pro Tyr Glu Ser Lys Gly Lys Gly Gly Phe Lys Arg Ser Ser
305 310 315 320
Leu Arg Phe Val Ala Val Ser Ser Arg Thr Arg Val Met Phe Tyr Ser
325 330 335
Thr Phe Tyr Ala Met Arg Asn Asp Asp Phe Ser Ser Leu Cys Gly Pro
340 345 350
Val Ile Asp Asp Val Lys Leu Leu Ser Ala Arg Arg Pro
355 360 365
<210>24
<211>129
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5G67070
<400>24
Met Ala Ala Ser Ser Leu Asn Leu Leu Leu Ile Leu Ser Leu Leu Thr
1 5 10 15
Phe Ile Ser Leu Gln Arg Ser Glu Ser Leu Ser Asp Asn Pro Ser Leu
20 25 30
Thr Leu Leu Pro Asp Gly Phe Asp Trp Pro Ile Ser His Ser Asp Glu
35 40 45
Phe Asp Ile Ile Asp Gly Glu Glu Ser Phe Glu Val Thr Glu Glu Asp
50 55 60
Asp Gly Val Thr Asp Arg Arg Ser Leu Tyr Trp Arg Arg Thr Lys Tyr
65 70 75 80
Tyr Ile Ser Tyr Gly Ala Leu Ser Ala Asn Arg Val Pro Cys Pro Pro
85 90 95
Arg Ser Gly Arg Ser Tyr Tyr Thr His Asn Cys Phe Arg Ala Arg Gly
100 105 110
Pro Val His Pro Tyr Ser Arg Gly Cys Ser Ser Ile Thr Arg Cys Arg
115 120 125
Arg
<210>25
<211>418
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At4G13210
<400>25
Met Val Val Ala Arg Thr Leu Phe Ser Ile Ser Ala Thr Leu Ile Ile
1 5 10 15
Phe Leu Ala Leu Phe Leu His Val Asn Ala Leu Ser Asp Gly Glu Trp
20 25 30
His Glu His Ala Val Lys Asp Pro Glu Glu Ile Ala Ala Met Val Asp
35 40 45
Met Ser Ile Arg Asn Ser Thr Tyr Arg Arg Lys Leu Gly Phe Phe Ser
50 55 60
Ser Cys Ser Thr Gly Asn Pro Ile Asp Asp Cys Trp Arg Cys Asp Lys
65 70 75 80
Lys Trp His Arg Arg Arg Lys Arg Leu Ala Asp Cys Ala Ile Gly Phe
85 90 95
Gly Arg Asn Ala Val Gly Gly Arg Asp Gly Arg Tyr Tyr Ile Val Thr
100 105 110
Asp Pro Ser Asp His Asp Pro Val Thr Pro Lys Pro Gly Thr Leu Arg
115 120 125
Tyr Ala Val Ile Gln Asp Glu Pro Leu Trp Ile Val Phe Lys Arg Asp
130 135 140
Met Val Ile Thr Leu Ser Gln Glu Leu Ile Met Asn Ser Phe Lys Thr
145 150 155 160
Ile Asp Gly Arg Gly Val Asn Val His Ile Ala Gly Gly Ala Cys Leu
165 170 175
Thr Val Gln Tyr Val Thr Asn Ile Ile Ile His Gly Ile Asn Ile His
180 185 190
Asp Cys Lys Arg Thr Gly Asn Ala Met Val Arg Ser Ser Glu Ser His
195 200 205
Tyr Gly Trp Arg Thr Met Ala Asp Gly Asp Gly Ile Ser Ile Phe Gly
210 215 220
Ser Ser His Ile Trp Ile Asp His Asn Ser Leu Ser Ser Cys Ala Asp
225 230 235 240
Gly Leu Ile Asp Ala Ile Met Gly Ser Thr Ala Ile Thr Ile Ser Asn
245 250 255
Asn Tyr Leu Thr His His Asn Glu Ala Ile Leu Leu Gly His Thr Asp
260 265 270
Ser Tyr Thr Arg Asp Lys Met Met Gln Val Thr Ile Ala Tyr Asn His
275 280 285
Phe Gly Glu Gly Leu Ile Gln Arg Met Pro Arg Cys Arg His Gly Tyr
290 295 300
Phe His Val Val Asn Asn Asp Tyr Thr His Trp Glu Met Tyr Ala Ile
305 310 315 320
Gly Gly Ser Ala Asn Pro Thr Ile Asn Ser Gln Gly Asn Arg Phe Leu
325 330 335
Ala Pro Gly Asn Arg Phe Ala Lys Glu Val Thr Lys Arg Val Gly Ala
340 345 350
Gly Lys Gly Glu Trp Asn Asn Trp Asn Trp Arg Ser Gln Gly Asp Leu
355 360 365
Met Leu Asn Gly Ala Tyr Phe Thr Ser Ser Gly Ala Gly Ala Ser Ala
370 375 380
Asn Tyr Ala Arg Ala Ser Ser Leu Ala Ala Lys Ser Ser Ser Leu Val
385 390 395 400
Gly Met Leu Thr Ser Ser Ser Gly Ala Leu Lys Cys Arg Ile Gly Thr
405 410 415
Leu Cys
<210>26
<211>461
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At1G80370
<400>26
Met Gly Lys Glu Asn Ala Val Ser Gly Asn Ser Ile Pro Ile His Gly
1 5 10 15
Arg Pro Val Thr Arg Ala Leu Ala Ser Ala Leu Arg Ala Ser Ser Lys
20 25 30
Leu Ile Thr Ser Ser Glu Val Ala Ala Thr Thr Gln Asn Gln Gly Arg
35 40 45
Val Leu Arg Ala Lys Ser Lys Arg Thr Ala Leu Asp Glu Lys Lys Ala
50 55 60
Asn Ala Pro Lys Lys Arg Ala Val Leu Lys Asp Ile Thr Asn Val Thr
65 70 75 80
Cys Glu Asn Ser Tyr Thr Ser Cys Phe Ser Val Ala Val Glu Asn Ile
85 90 95
Lys Gln Ile Lys Lys Gly Arg Gln Ser Ser Ser Ser Ser Lys Val Ala
100 105 110
Ser Ser Ser Ala Thr Ser Gln Val Thr Asp Ala Lys Val Glu Val Val
115 120 125
Ser Asn Ser Ala Gly Ala Ser Leu Ser Val Phe Thr Asp Thr Ser Leu
130 135 140
Gly Thr Asn Glu Thr Ser Tyr Ser Ile Ile Ala Lys Pro Ser Ser Arg
145 150 155 160
Ser Pro Pro Arg Pro Phe Gly Thr Val Glu Arg Ser Cys Gly Gly Ala
165 170 175
Ser Ser Pro Lys Phe Val Asp Ile Asp Ser Asp Asp Lys Asp Pro Leu
180 185 190
Leu Cys Ser Leu Tyr Ala Pro Asp Ile Tyr Tyr Asn Leu Arg Val Ala
195 200 205
Glu Leu Lys Arg Arg Pro Phe Pro Asp Phe Met Glu Lys Thr Gln Arg
210 215 220
Asp Val Thr Glu Thr Met Arg Gly Ile Leu Val Asp Trp Leu Val Glu
225 230 235 240
Val Ser Glu Glu Tyr Thr Leu Val Pro Asp Thr Leu Tyr Leu Thr Val
245 250 255
Tyr Leu Ile Asp Trp Phe Leu His Gly Asn Tyr Val Glu Arg Gln Arg
260 265 270
Leu Gln Leu Leu Gly Ile Thr Cys Met Leu Ile Ala Ser Lys Tyr Glu
275 280 285
Glu Ile His Ala Pro Arg Ile Glu Glu Phe Cys Phe Ile Thr Asp Asn
290 295 300
Thr Tyr Thr Arg Asp Gln Val Leu Glu Met Glu Ser Gln Val Leu Lys
305 310 315 320
His Phe Ser Phe Gln Ile Tyr Thr Pro Thr Ser Lys Thr Phe Leu Arg
325 330 335
Arg Phe Leu Arg Ala Ala Gln Val Ser Phe Pro Asn Gln Ser Leu Glu
340 345 350
Met Glu Phe Leu Ala Asn Tyr Leu Thr Glu Leu Thr Leu Met Asp Tyr
355 360 365
Pro Phe Leu Lys Phe Leu Pro Ser Ile Ile Ala Ala Ser Ala Val Phe
370 375 380
Leu Ala Lys Trp Thr Leu Asn Gln Ser Ser His Pro Trp Asn Pro Thr
385 390 395 400
Leu Glu His Tyr Thr Thr Tyr Lys Ala Ser Asp Leu Lys Ala Ser Val
405 410 415
His Ala Leu Gln Asp Leu Gln Leu Asn Thr Lys Gly Cys Ser Leu Asn
420 425 430
Ser Ile Arg Met Lys Tyr Arg Gln Asp Lys Phe Lys Ser Val Ala Val
435 440 445
Phe Ser Ser Gly Glu Leu Pro Asp Lys Leu Phe Ile Ser
450 455 460
<210>27
<211>167
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At3G01070
<400>27
Met Ala Arg Val Ala Val Leu Val Ala Gly Ala Val Leu Ala Phe Leu
1 5 10 15
Leu Ala Ala Thr Asn Val Thr Ala Lys Arg Trp Thr Val Gly Asp Asn
20 25 30
Lys Phe Trp Asn Pro Asn Ile Asn Tyr Thr Ile Trp Ala Gln Asp Lys
35 40 45
His Phe Tyr Leu Asp Asp Trp Leu Tyr Phe Val Tyr Glu Arg Asn Gln
50 55 60
Tyr Asn Val Ile Glu Val Asn Glu Thr Asn Tyr Ile Ser Cys Asn Pro
65 70 75 80
Asn Asn Pro Ile Ala Asn Trp Ser Arg Gly Ala Gly Arg Asp Leu Val
85 90 95
His Leu Asn Val Thr Arg His Tyr Tyr Leu Ile Ser Gly Asn Gly Gly
100 105 110
Gly Cys Tyr Gly Gly Met Lys Leu Ala Val Leu Val Glu Lys Pro Pro
115 120 125
Pro Pro Pro Ala Ala Ala Pro Asn Lys Asn Ser Ala Arg Arg Thr Phe
130 135 140
Ser Val Ser Gly Phe Ala Tyr Gln Phe Leu Ile Pro Val Ala Val Phe
145 150 155 160
Ala Ala Val Gly Thr Arg Tyr
165
<210>28
<211>390
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At1G61580
<400>28
Met Ser His Arg Lys Phe Glu His Pro Arg His Gly Ser Leu Gly Phe
1 5 10 15
Leu Pro Arg Lys Arg Ala Ser Arg His Arg Gly Lys Val Lys Ala Phe
20 25 30
Pro Lys Asp Asp Pro Thr Lys Pro Cys Arg Leu Thr Ser Phe Leu Gly
35 40 45
Tyr Lys Ala Gly Met Thr His Ile Val Arg Asp Val Glu Lys Pro Gly
50 55 60
Ser Lys Leu His Lys Lys Glu Thr Cys Glu Ala Val Thr Ile Ile Glu
65 70 75 80
Thr Pro Pro Met Val Val Val Gly Val Val Gly Tyr Val Lys Thr Pro
85 90 95
Arg Gly Leu Arg Ser Leu Cys Thr Val Trp Ala Gln His Leu Ser Glu
100 105 110
Glu Leu Arg Arg Arg Phe Tyr Lys Asn Trp Ala Lys Ser Lys Lys Lys
115 120 125
Ala Phe Thr Arg Tyr Ser Lys Lys His Glu Thr Glu Glu Gly Lys Lys
130 135 140
Asp Ile Gln Ser Gln Leu Glu Lys Met Lys Lys Tyr Cys Ser Val Ile
145 150 155 160
Arg Val Leu Ala His Thr Gln Ile Arg Lys Met Lys Gly Leu Lys Gln
165 170 175
Lys Lys Ala His Leu Asn Glu Ile Gln Ile Asn Gly Gly Asp Ile Ala
180 185 190
Lys Lys Val Asp Tyr Ala Cys Ser Leu Phe Glu Lys Gln Val Pro Val
195 200 205
Asp Ala Ile Phe Gln Lys Asp Glu Met Ile Asp Ile Ile Gly Val Thr
210 215 220
Lys Gly Lys Gly Tyr Glu Gly Val Val Thr Arg Trp Gly Val Thr Arg
225 230 235 240
Leu Pro Arg Lys Thr His Arg Gly Leu Arg Lys Val Ala Cys Ile Gly
245 250 255
Ala Trp His Pro Ala Arg Val Ser Tyr Thr Val Ala Arg Ala Gly Gln
260 265 270
Asn Gly Tyr His His Arg Thr Glu Met Asn Lys Lys Val Tyr Arg Val
275 280 285
Gly Lys Val Gly Gln Glu Thr His Ser Ala Met Thr Glu Tyr Asp Arg
290 295 300
Thr Glu Lys Asp Ile Thr Pro Met Gly Gly Phe Pro His Tyr Gly Ile
305 310 315 320
Val Lys Glu Asp Tyr Leu Met Ile Lys Gly Cys Cys Val Gly Pro Lys
325 330 335
Lys Arg Val Val Thr Leu Arg Gln Thr Leu Leu Lys Gln Thr Ser Arg
340 345 350
Leu Ala Met Glu Glu Ile Lys Leu Lys Phe Ile Asp Ala Ala Ser Asn
355 360 365
Gly Gly His Gly Arg Phe Gln Thr Ser Gln Glu Lys Ala Lys Phe Tyr
370 375 380
Gly Arg Thr Ile Lys Ala
385 390
<210>29
<211>175
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At2G46990
<400>29
Met Gly Arg Gly Arg Ser Ser Ser Ser Ser Ser Ile Glu Ser Ser Ser
1 5 10 15
Lys Ser Asn Pro Phe Gly Ala Ser Ser Ser Thr Arg Asn Leu Ser Thr
20 25 30
Asp Leu Arg Leu Gly Leu Ser Phe Gly Thr Ser Ser Gly Thr Gln Tyr
35 40 45
Phe Asn Gly Gly Tyr Gly Tyr Ser Val Ala Ala Pro Ala Val Glu Asp
50 55 60
Ala Glu Tyr Val Ala Ala Val Glu Glu Glu Glu Glu Asn Glu Cys Asn
65 70 75 80
Ser Val Gly Ser Phe Tyr Val Lys Val Asn Met Glu Gly Val Pro Ile
85 90 95
Gly Arg Lys Ile Asp Leu Met Ser Leu Asn Gly Tyr Arg Asp Leu Ile
100 105 110
Arg Thr Leu Asp Phe Met Phe Asn Ala Ser Ile Leu Trp Ala Glu Glu
115 120 125
Glu Asp Met Cys Asn Glu Lys Ser His Val Leu Thr Tyr Ala Asp Lys
130 135 140
Glu Gly Asp Trp Met Met Val Gly Asp Val Pro Trp Glu Met Phe Leu
145 150 155 160
Ser Thr Val Arg Arg Leu Lys Ile Ser Arg Ala Asn Tyr His Tyr
165 170 175
<210>30
<211>716
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At4G02060
<400>30
Met Lys Asp His Asp Phe Asp Gly Asp Lys Gly Leu Ala Lys Gly Phe
1 5 10 15
Leu Glu Asn Phe Ala Asp Ala Asn Gly Arg Ser Lys Tyr Met Glu Ile
20 25 30
Leu Gln Glu Val Ser Asn Arg Lys Ile Arg Ala Ile Gln Val Asp Leu
35 40 45
Asp Asp Leu Phe Asn Tyr Lys Asp Glu Ser Glu Glu Phe Leu Gly Arg
50 55 60
Leu Thr Glu Asn Thr Arg Arg Tyr Val Ser Ile Phe Ser Ala Ala Val
65 70 75 80
Asp Glu Leu Leu Pro Glu Pro Thr Glu Ala Phe Pro Asp Asp Asp His
85 90 95
Asp Ile Leu Met Thr Gln Arg Ala Asp Asp Gly Thr Asp Asn Pro Asp
100 105 110
Val Ser Asp Pro His Gln Gln Ile Pro Ser Glu Ile Lys Arg Tyr Tyr
115 120 125
Glu Val Tyr Phe Lys Ala Pro Ser Lys Gly Arg Pro Ser Thr Ile Arg
130 135 140
Glu Val Lys Ala Ser His Ile Gly Gln Leu Val Arg Ile Ser Gly Ile
145 150 155 160
Val Thr Arg Cys Ser Asp Val Lys Pro Leu Met Ala Val Ala Val Tyr
165 170 175
Thr Cys Glu Asp Cys Gly His Glu Ile Tyr Gln Glu Val Thr Ser Arg
180 185 190
Val Phe Met Pro Leu Phe Lys Cys Pro Ser Ser Arg Cys Arg Leu Asn
195 200 205
Ser Lys Ala Gly Asn Pro Ile Leu Gln Leu Arg Ala Ser Lys Phe Leu
210 215 220
Lys Phe Gln Glu Ala Lys Met Gln Glu Leu Ala Glu His Val Pro Lys
225 230 235 240
Gly His Ile Pro Arg Ser Met Thr Val His Leu Arg Gly Glu Leu Thr
245 250 255
Arg Lys Val Ser Pro Gly Asp Val Val Glu Phe Ser Gly Ile Phe Leu
260 265 270
Pro Ile Pro Tyr Thr Gly Phe Lys Ala Leu Arg Ala Gly Leu Val Ala
275 280 285
Asp Thr Tyr Leu Glu Ala Thr Ser Val Thr His Phe Lys Lys Lys Tyr
290 295 300
Glu Glu Tyr Glu Phe Gln Lys Asp Glu Glu Glu Gln Ile Ala Arg Leu
305 310 315 320
Ala Glu Asp Gly Asp Ile Tyr Asn Lys Leu Ser Arg Ser Leu Ala Pro
325 330 335
Glu Ile Tyr Gly His Glu Asp Ile Lys Lys Ala Leu Leu Leu Leu Leu
340 345 350
Val Gly Ala Pro His Arg Gln Leu Lys Asp Gly Met Lys Ile Arg Gly
355 360 365
Asp Val His Ile Cys Leu Met Gly Asp Pro Gly Val Ala Lys Ser Gln
370 375 380
Leu Leu Lys His Ile Ile Asn Val Ala Pro Arg Gly Val Tyr Thr Thr
385 390 395 400
Gly Lys Gly Ser Ser Gly Val Gly Leu Thr Ala Ala Val Met Arg Asp
405 410 415
Gln Val Thr Asn Glu Met Val Leu Glu Gly Gly Ala Leu Val Leu Ala
420 425 430
Asp Met Gly Ile Cys Ala Ile Asp Glu Phe Asp Lys Met Asp Glu Ser
435 440 445
Asp Arg Thr Ala Ile His Glu Val Met Glu Gln Gln Thr Val Ser Ile
450 455 460
Ala Lys Ala Gly Ile Thr Thr Ser Leu Asn Ala Arg Thr Ala Val Leu
465 470 475 480
Ala Ala Ala Asn Pro Ala Trp Gly Arg Tyr Asp Leu Arg Arg Thr Pro
485 490 495
Ala Glu Asn Ile Asn Leu Pro Pro Ala Leu Leu Ser Arg Phe Asp Leu
500 505 510
Leu Trp Leu Ile Leu Asp Arg Ala Asp Met Asp Ser Asp Leu Glu Leu
515 520 525
Ala Lys His Val Leu His Val His Gln Thr Glu Glu Ser Pro Ala Leu
530 535 540
Gly Phe Glu Pro Leu Glu Pro Asn Ile Leu Arg Ala Tyr Ile Ser Ala
545 550 555 560
Ala Arg Arg Leu Ser Pro Tyr Val Pro Ala Glu Leu Glu Glu Tyr Ile
565 570 575
Ala Thr Ala Tyr Ser Ser Ile Arg Gln Glu Glu Ala Lys Ser Asn Thr
580 585 590
Pro His Ser Tyr Thr Thr Val Arg Thr Leu Leu Ser Ile Leu Arg Ile
595 600 605
Ser Ala Ala Leu Ala Arg Leu Arg Phe Ser Glu Ser Val Ala Gln Ser
610 615 620
Asp Val Asp Glu Ala Leu Arg Leu Met Gln Met Ser Lys Ile Ser Leu
625 630 635 640
Tyr Ala Asp Asp Arg Gln Lys Ala Gly Leu Asp Ala Ile Ser Asp Thr
645 650 655
Tyr Ser Ile Ile Arg Asp Glu Ala Ala Arg Ser Lys Lys Thr His Val
660 665 670
Ser Tyr Ala Asn Ala Leu Asn Trp Ile Ser Arg Lys Gly Tyr Ser Glu
675 680 685
Ala Gln Leu Lys Glu Cys Leu Glu Glu Tyr Ala Ala Leu Asn Val Trp
690 695 700
Gln Ile Asp Pro His Thr Phe Asp Ile Arg Phe Ile
705 710 715
<210>31
<211>351
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At2G33620
<400>31
Met Ser Gly Ser Glu Thr Gly Leu Met Ala Ala Thr Arg Glu Ser Met
1 5 10 15
Gln Phe Thr Met Ala Leu His Gln Gln Gln Gln His Ser Gln Ala Gln
20 25 30
Pro Gln Gln Ser Gln Asn Arg Pro Leu Ser Phe Gly Gly Asp Asp Gly
35 40 45
Thr Ala Leu Tyr Lys Gln Pro Met Arg Ser Val Ser Pro Pro Gln Gln
50 55 60
Tyr Gln Pro Asn Ser Ala Gly Glu Asn Ser Val Leu Asn Met Asn Leu
65 70 75 80
Pro Gly Gly Glu Ser Gly Gly Met Thr Gly Thr Gly Ser Glu Pro Val
85 90 95
Lys Lys Arg Arg Gly Arg Pro Arg Lys Tyr Gly Pro Asp Ser Gly Glu
100 105 110
Met Ser Leu Gly Leu Asn Pro Gly Ala Pro Ser Phe Thr Val Ser Gln
115 120 125
Pro Ser Ser Gly Gly Asp Gly Gly Glu Lys Lys Arg Gly Arg Pro Pro
130 135 140
Gly Ser Ser Ser Lys Arg Leu Lys Leu Gln Ala Leu Gly Ser Thr Gly
145 150 155 160
Ile Gly Phe Thr Pro His Val Leu Thr Val Leu Ala Gly Glu Asp Val
165 170 175
Ser Ser Lys Ile Met Ala Leu Thr His Asn Gly Pro Arg Ala Val Cys
180 185 190
Val Leu Ser Ala Asn Gly Ala Ile Ser Asn Val Thr Leu Arg Gln Ser
195 200 205
Ala Thr Ser Gly Gly Thr Val Thr Tyr Glu Gly Arg Phe Glu Ile Leu
210 215 220
Ser Leu Ser Gly Ser Phe His Leu Leu Glu Asn Asn Gly Gln Arg Ser
225 230 235 240
Arg Thr Gly Gly Leu Ser Val Ser Leu Ser Ser Pro Asp Gly Asn Val
245 250 255
Leu Gly Gly Ser Val Ala Gly Leu Leu Ile Ala Ala Ser Pro Val Gln
260 265 270
Ile Val Val Gly Ser Phe Leu Pro Asp Gly Glu Lys Glu Pro Lys Gln
275 280 285
His Val Gly Gln Met Gly Leu Ser Ser Pro Val Leu Pro Arg Val Ala
290 295 300
Pro Thr Gln Val Leu Met Thr Pro Ser Ser Pro Gln Ser Arg Gly Thr
305 310 315 320
Met Ser Glu Ser Ser Cys Gly Gly Gly His Gly Ser Pro Ile His Gln
325 330 335
Ser Thr Gly Gly Pro Tyr Asn Asn Thr Ile Asn Met Pro Trp Lys
340 345 350
<210>32
<211>1492
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At5G67100
<400>32
Met Ser Gly Asp Asn Ser Thr Glu Thr Gly Arg Arg Arg Ser Arg Gly
1 5 10 15
Ala Glu Ala Ser Ser Arg Lys Asp Thr Leu Glu Arg Leu Lys Ala Ile
20 25 30
Arg Gln Gly Gly Ile Arg Ser Ala Ser Gly Gly Gly Tyr Asp Ile Arg
35 40 45
Leu Gln Lys Pro Ile Phe Asp Thr Val Asp Asp Glu Glu Tyr Asp Ala
50 55 60
Leu Val Ser Arg Arg Arg Glu Glu Ala Arg Gly Phe Val Val Glu Asp
65 70 75 80
Gly Glu Gly Gly Asp Leu Gly Tyr Leu Asp Glu Gly Glu Glu Glu Asp
85 90 95
Trp Ser Lys Pro Ser Gly Pro Glu Ser Thr Asp Glu Ser Asp Asp Gly
100 105 110
Gly Arg Phe Ser Gly Arg Leu Lys Lys Lys Lys Lys Gly Lys Glu Gln
115 120 125
Thr Gln Gln Pro Gln Val Lys Lys Val Asn Pro Ala Leu Lys Ala Ala
130 135 140
Ala Thr Ile Thr Gly Glu Gly Arg Leu Ser Ser Met Phe Thr Ser Ser
145 150 155 160
Ser Phe Lys Lys Val Lys Glu Thr Asp Lys Ala Gln Tyr Glu Gly Ile
165 170 175
Leu Asp Glu Ile Ile Ala Gln Val Thr Pro Asp Glu Ser Asp Arg Lys
180 185 190
Lys His Thr Arg Arg Lys Leu Pro Gly Thr Val Pro Val Thr Ile Phe
195 200 205
Lys Asn Lys Lys Leu Phe Ser Val Ala Ser Ser Met Gly Met Lys Glu
210 215 220
Ser Glu Pro Thr Pro Ser Thr Tyr Glu Gly Asp Ser Val Ser Met Asp
225 230 235 240
Asn Glu Leu Met Lys Glu Glu Asp Met Lys Glu Ser Glu Val Ile Pro
245 250 255
Ser Glu Thr Met Glu Leu Leu Gly Ser Asp Ile Val Lys Glu Asp Gly
260 265 270
Ser Asn Lys Ile Arg Lys Thr Glu Val Lys Ser Glu Leu Gly Val Lys
275 280 285
Glu Val Phe Thr Leu Asn Ala Thr Ile Asp Met Lys Glu Lys Asp Ser
290 295 300
Ala Leu Ser Ala Thr Ala Gly Trp Lys Glu Ala Met Gly Lys Val Gly
305 310 315 320
Thr Glu Asn Gly Ala Leu Leu Gly Ser Ser Ser Glu Gly Lys Thr Glu
325 330 335
Phe Asp Leu Asp Ala Asp Gly Ser Leu Arg Phe Phe Ile Leu Asp Ala
340 345 350
Tyr Glu Glu Ala Phe Gly Ala Ser Met Gly Thr Ile Tyr Leu Phe Gly
355 360 365
Lys Val Lys Met Gly Asp Thr Tyr Lys Ser Cys Cys Val Val Val Lys
370 375 380
Asn Ile Gln Arg Cys Val Tyr Ala Ile Pro Asn Asp Ser Ile Phe Pro
385 390 395 400
Ser His Glu Leu Ile Met Leu Glu Gln Glu Val Lys Asp Ser Arg Leu
405 4l0 415
Ser Pro Glu Ser Phe Arg Gly Lys Leu His Glu Met Ala Ser Lys Leu
420 425 430
Lys Asn Glu Ile Ala Gln Glu Leu Leu Gln Leu Asn Val Ser Asn Phe
435 440 445
Ser Met Ala Pro Val Lys Arg Asn Tyr Ala Phe Glu Arg Pro Asp Val
450 455 460
Pro Ala Gly Glu Gln Tyr Val Leu Lys Ile Asn Tyr Ser Phe Lys Asp
465 470 475 480
Arg Pro Leu Pro Glu Asp Leu Lys Gly Glu Ser Phe Ser Ala Leu Leu
485 490 495
Gly Ser His Thr Ser Ala Leu Glu His Phe Ile Leu Lys Arg Lys Ile
500 505 510
Met Gly Pro Cys Trp Leu Lys Ile Ser Ser Phe Ser Thr Cys Ser Pro
515 520 525
Ser Glu Gly Val Ser Trp Cys Lys Phe Glu Val Thr Val Gln Ser Pro
530 535 540
Lys Asp Ile Thr Ile Leu Val Ser Glu Glu Lys Val Val His Pro Pro
545 550 555 560
Ala Val Val Thr Ala Ile Asn Leu Lys Thr Ile Val Asn Glu Lys Gln
565 570 575
Asn Ile Ser Glu Ile Val Ser Ala Ser Val Leu Cys Phe His Asn Ala
580 585 590
Lys Ile Asp Val Pro Met Pro Ala Pro Glu Arg Lys Arg Ser Gly Ile
595 600 605
Leu Ser His Phe Thr Val Val Arg Asn Pro Glu Gly Thr Gly Tyr Pro
610 615 620
Ile Gly Trp Lys Lys Glu Val Ser Asp Arg Asn Ser Lys Asn Gly Cys
625 630 635 640
Asn Val Leu Ser Ile Glu Asn Ser Glu Arg Ala Leu Leu Asn Arg Leu
645 650 655
Phe Leu Glu Leu Asn Lys Leu Asp Ser Asp Ile Leu Val Gly His Asn
660 665 670
Ile Ser Gly Phe Asp Leu Asp Val Leu Leu Gln Arg Ala Gln Ala Cys
675 680 685
Lys Val Gln Ser Ser Met Trp Ser Lys Ile Gly Arg Leu Lys Arg Ser
690 695 700
Phe Met Pro Lys Leu Lys Gly Asn Ser Asn Tyr Gly Ser Gly Ala Thr
705 710 715 720
Pro Gly Leu Met Ser Cys Ile Ala Gly Arg Leu Leu Cys Asp Thr Asp
725 730 735
Leu Cys Ser Arg Asp Leu Leu Lys Glu Val Ser Tyr Ser Leu Thr Asp
740 745 750
Leu Ser Lys Thr Gln Leu Asn Arg Asp Arg Lys Glu Ile Ala Pro Asn
755 760 765
Asp Ile Pro Lys Met Phe Gln Ser Ser Lys Thr Leu Val Glu Leu Ile
770 775 780
Glu Cys Gly Glu Thr Asp Ala Trp Leu Ser Met Glu Leu Met Phe His
785 790 795 800
Leu Ser Val Leu Pro Leu Thr Leu Gln Leu Thr Asn Ile Ser Gly Asn
805 810 815
Leu Trp Gly Lys Thr Leu Gln Gly Ala Arg Ala Gln Arg Ile Glu Tyr
820 825 830
Tyr Leu Leu His Thr Phe His Ser Lys Lys Phe Ile Leu Pro Asp Lys
835 840 845
Ile Ser Gln Arg Met Lys Glu Ile Lys Ser Ser Lys Arg Arg Met Asp
850 855 860
Tyr Ala Pro Glu Asp Arg Asn Val Asp Glu Leu Asp Ala Asp Leu Thr
865 870 875 880
Leu Glu Asn Asp Pro Ser Lys Gly Ser Lys Thr Lys Lys Gly Pro Ala
885 890 895
Tyr Ala Gly Gly Leu Val Leu Glu Pro Lys Arg Gly Leu Tyr Asp Lys
900 905 910
Tyr Val Leu Leu Leu Asp Phe Asn Ser Leu Tyr Pro Ser Ile Ile Gln
915 920 925
Glu Tyr Asn Ile Cys Phe Thr Thr Ile Pro Arg Ser Glu Asp Gly Val
930 935 940
Pro Arg Leu Pro Ser Ser Gln Thr Pro Gly Ile Leu Pro Lys Leu Met
945 950 955 960
Glu His Leu Val Ser Ile Arg Lys Ser Val Lys Leu Lys Met Lys Lys
965 970 975
Glu Thr Gly Leu Lys Tyr Trp Glu Leu Asp Ile Arg Gln Gln Ala Leu
980 985 990
Lys Leu Thr Ala Asn Ser Met Tyr Gly Cys Leu Gly Phe Ser Asn Ser
995 1000 1005
Arg Phe Tyr Ala Lys Pro Leu Ala Glu Leu Ile Thr Leu Gln Gly
1010 1015 1020
Arg Asp Ile Leu Gln Arg Thr Val Asp Leu Val Gln Asn His Leu
1025 1030 1035
Asn Leu Glu Val Ile Tyr Gly Asp Thr Asp Ser Ile Met Ile His
1040 1045 1050
Ser Gly Leu Asp Asp Ile Glu Glu Val Lys Ala Ile Lys Ser Lys
1055 1060 1065
Val Ile Gln Glu Val Asn Lys Lys Tyr Arg Cys Leu Lys Ile Asp
1070 1075 1080
Cys Asp Gly Ile Tyr Lys Arg Met Leu Leu Leu Arg Lys Lys Lys
1085 1090 1095
Tyr Ala Ala Val Lys Leu Gln Phe Lys Asp Gly Lys Pro Cys Glu
1100 1105 1110
Asp Ile Glu Arg Lys Gly Val Asp Met Val Arg Arg Asp Trp Ser
1115 1120 1125
Leu Leu Ser Lys Glu Ile Gly Asp Leu Cys Leu Ser Lys Ile Leu
1130 1135 1140
Tyr Gly Gly Ser Cys Glu Asp Val Val Glu Ala Ile His Asn Glu
1145 1150 1155
Leu Met Lys Ile Lys Glu Glu Met Arg Asn Gly Gln Val Ala Leu
1160 1165 1170
Glu Lys Tyr Val Ile Thr Lys Thr Leu Thr Lys Pro Pro Ala Ala
1175 1180 1185
Tyr Pro Asp Ser Lys Ser Gln Pro His Val Gln Val Ala Leu Arg
1190 1195 1200
Met Arg Gln Arg Gly Tyr Lys Glu Gly Phe Asn Ala Lys Asp Thr
1205 1210 1215
Val Pro Tyr Ile Ile Cys Tyr Glu Gln Gly Asn Ala Ser Ser Ala
1220 1225 1230
Ser Ser Ala Gly Ile Ala Glu Arg Ala Arg His Pro Asp Glu Val
1235 1240 1245
Lys Ser Glu Gly Ser Arg Trp Leu Val Asp Ile Asp Tyr Tyr Leu
1250 1255 1260
Ala Gln Gln Ile His Pro Val Val Ser Arg Leu Cys Ala Glu Ile
1265 1270 1275
Gln Gly Thr Ser Pro Glu Arg Leu Ala Glu Cys Leu Gly Leu Asp
1280 1285 1290
Pro Ser Lys Tyr Arg Ser Lys Ser Asn Asp Ala Thr Ser Ser Asp
1295 1300 1305
Pro Ser Thr Ser Leu Leu Phe Ala Thr Ser Asp Glu Glu Ser Lys
1310 1315 1320
Lys Pro Ala Thr Pro Glu Thr Glu Glu Ser Asp Ser Thr Phe Trp
1325 1330 1335
Leu Lys Leu His Cys Pro Lys Cys Gln Gln Glu Asp Ser Thr Gly
1340 1345 1350
Ile Ile Ser Pro Ala Met Ile Ala Asn Gln Val Lys Arg Gln Ile
1355 1360 1365
Asp Gly Phe Val Ser Met Tyr Tyr Lys Gly Ile Met Val Cys Glu
1370 1375 1380
Asp Glu Ser Cys Lys His Thr Thr Arg Ser Pro Asn Phe Arg Leu
1385 1390 1395
Leu Gly Glu Arg Glu Arg Gly Thr Val Cys Pro Asn Tyr Pro Asn
1400 1405 1410
Cys Asn Gly Thr Leu Leu Arg Lys Tyr Thr Glu Ala Asp Leu Tyr
1415 1420 1425
Lys Gln Leu Ser Tyr Phe Cys His Ile Leu Asp Thr Gln Cys Ser
1430 1435 1440
Leu Glu Lys Met Asp Val Gly Val Arg Ile Gln Val Glu Lys Ala
1445 1450 1455
Met Thr Lys Ile Arg Pro Ala Val Lys Ser Ala Ala Ala Ile Thr
1460 1465 1470
Arg Ser Ser Arg Asp Arg Cys Ala Tyr Gly Trp Met Gln Leu Thr
1475 1480 1485
Asp Ile Val Ile
1490
<210>33
<211>895
<212>PRT
<213>拟南芥
<220>
<221>MISC_FEATURE
<223>At3G59420
<400>33
Met Arg Met Phe Glu Thr Arg Ala Arg Glu Trp Ile Leu Leu Val Lys
1 5 10 15
Leu Val Leu Phe Thr Ser Ile Trp Gln Leu Ala Ser Ala Leu Gly Ser
20 25 30
Met Ser Ser Ile Ala Ile Ser Tyr Gly Glu Gly Gly Ser Val Phe Cys
35 40 45
Gly Leu Lys Ser Asp Gly Ser His Leu Val Val Cys Tyr Gly Ser Asn
50 55 60
Ser Ala Ile Leu Tyr Gly Thr Pro Gly His Leu Gln Phe Ile Gly Leu
65 70 75 80
Thr Gly Gly Asp Gly Phe Met Cys Gly Leu Leu Met Leu Ser His Gln
85 90 95
Pro Tyr Cys Trp Gly Asn Ser Ala Phe Ile Gln Met Gly Val Pro Gln
100 105 110
Pro Met Thr Lys Gly Ala Glu Tyr Leu Glu Val Ser Ala Gly Asp Tyr
115 120 125
His Leu Cys Gly Leu Arg Lys Pro Ile Val Gly Arg Arg Lys Asn Ser
130 135 140
Asn Ile Ile Ser Ser Ser Leu Val Asp Cys Trp Gly Tyr Asn Met Thr
145 150 155 160
Arg Asn Phe Val Phe Asp Lys Gln Leu His Ser Leu Ser Ala Gly Ser
165 170 175
Glu Phe Asn Cys Ala Leu Ser Ser Lys Asp Lys Ser Val Phe Cys Trp
180 185 190
Gly Asp Glu Asn Ser Ser Gln Val Ile Ser Leu Ile Pro Lys Glu Lys
195 200 205
Lys Phe Gln Lys Ile Ala Ala Gly Gly Tyr His Val Cys Gly Ile Leu
210 215 220
Asp Gly Leu Glu Ser Arg Val Leu Cys Trp Gly Lys Ser Leu Glu Phe
225 230 235 240
Glu Glu Glu Val Thr Gly Thr Ser Thr Glu Glu Lys Ile Leu Asp Leu
245 250 255
Pro Pro Lys Glu Pro Leu Leu Ala Val Val Gly Gly Lys Phe Tyr Ala
260 265 270
Cys Gly Ile Lys Arg Tyr Asp His Ser Ala Val Cys Trp Gly Phe Phe
275 280 285
Val Asn Arg Ser Thr Pro Ala Pro Thr Gly Ile Gly Phe Tyr Asp Leu
290 295 300
Ala Ala Gly Asn Tyr Phe Thr Cys Gly Val Leu Thr Gly Thr Ser Met
305 310 315 320
Ser Pro Val Cys Trp Gly Leu Gly Phe Pro Ala Ser Ile Pro Leu Ala
325 330 335
Val Ser Pro Gly Leu Cys Ile Asp Thr Pro Cys Pro Pro Gly Thr His
340 345 350
Glu Leu Ser Asn Gln Glu Asn Ser Pro Cys Lys Phe Thr Gly Ser His
355 360 365
Ile Cys Leu Pro Cys Ser Thr Ser Cys Pro Pro Gly Met Tyr Gln Lys
370 375 380
Ser Val Cys Thr Glu Arg Ser Asp Gln Val Cys Val Tyr Asn Cys Ser
385 390 395 400
Ser Cys Ser Ser His Asp Cys Ser Ser Asn Cys Ser Ser Ser Ala Thr
405 410 415
Ser Gly Gly Lys Glu Lys Gly Lys Phe Trp Ser Leu Gln Leu Pro Ile
420 425 430
Ala Thr Ala Glu Ile Gly Phe Ala Leu Phe Leu Val Ala Val Val Ser
435 440 445
Ile Thr Ala Ala Leu Tyr Ile Arg Tyr Arg Leu Arg Asn Cys Arg Cys
450 455 460
Ser Glu Asn Asp Thr Arg Ser Ser Lys Asp Ser Ala Phe Thr Lys Asp
465 470 475 480
Asn Gly Lys Ile Arg Pro Asp Leu Asp Glu Leu Gln Lys Arg Arg Arg
485 490 495
Ala Arg Val Phe Thr Tyr Glu Glu Leu Glu Lys Ala Ala Asp Gly Phe
500 505 510
Lys Glu Glu Ser Ile Val Gly Lys Gly Ser Phe Ser Cys Val Tyr Lys
515 520 525
Gly Val Leu Arg Asp Gly Thr Thr Val Ala Val Lys Arg Ala Ile Met
530 535 540
Ser Ser Asp Lys Gln Lys Asn Ser Asn Glu Phe Arg Thr Glu Leu Asp
545 550 555 560
Leu Leu Ser Arg Leu Asn His Ala His Leu Leu Ser Leu Leu Gly Tyr
565 570 575
Cys Glu Glu Cys Gly Glu Arg Leu Leu Val Tyr Glu Phe Met Ala His
580 585 590
Gly Ser Leu His Asn His Leu His Gly Lys Asn Lys Ala Leu Lys Glu
595 600 605
Gln Leu Asp Trp Val Lys Arg Val Thr Ile Ala Val Gln Ala Ala Arg
610 615 620
Gly Ile Glu Tyr Leu His Gly Tyr Ala Cys Pro Pro Val Ile His Arg
625 630 635 640
Asp Ile Lys Ser Ser Asn Ile Leu Ile Asp Glu Glu His Asn Ala Arg
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Pro Leu Ala Glu Leu Pro Ala Gly Thr Leu Gly Tyr Leu Asp Pro Glu
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Ala Gly Asp Ile Asn Ala Leu Leu Asp Pro Val Leu Lys His Pro Ser
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Glu Ile Glu Ala Leu Lys Arg Ile Val Ser Val Ala Cys Lys Cys Val
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Leu Glu Arg Ala Leu Ala Gln Leu Met Gly Asn Pro Ser Ser Glu Gln
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Pro Ile Leu Pro Thr Glu Val Val Leu Gly Ser Ser Arg Met His Lys
805 810 815
Lys Ser Trp Arg Ile Gly Ser Lys Arg Ser Gly Ser Glu Asn Thr Glu
820 825 830
Phe Arg Gly Gly Ser Trp Ile Thr Phe Pro Ser Val Thr Ser Ser Gln
835 840 845
Arg Arg Lys Ser Ser Ala Ser Glu Gly Asp Val Ala Glu Glu Glu Asp
850 855 860
Glu Gly Arg Lys Gln Gln Glu Ala Leu Arg Ser Leu Glu Glu Glu Ile
865 870 875 880
Gly Pro Ala Ser Pro Gly Gln Ser Leu Phe Leu His His Asn Phe
885 890 895
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Asp Asn Pro Ser Ser Ser Ser Phe Glu Phe His Val Ser Leu Glu Glu
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325 330 335
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420 425 430
Pro Asn Thr Glu Glu Gln Glu Lys Ser Glu Val Leu Ser Glu Gln Ala
435 440 445
Arg Ser Glu
450
Claims (16)
1.分离涉及不对称细胞分裂的基因的方法，其包括：
(1)将野生型植物的根进行以同步方式诱导侧根起始的处理；(2)将由于生长素信号传递缺陷而不产生侧根的突变体的根进行以同步方式诱导野生型中侧根起始的处理；(3)鉴定在野生型中被诱导但是在突变体中不被诱导的基因。
2.根据权利要求1的方法，其中所述突变体是slr-1。
3.权利要求1或2的方法，其还包括使用木质部极中柱鞘标记株系，接着进行细胞分选。
4.用根据权利要求1-3任一项的方法分离的涉及不对称细胞分裂的基因。
5.根据权利要求4的基因，其中所述不对称细胞分裂是侧根形成。
6.根据权利要求5的基因，其中所述侧根形成是早期侧根形成。
7.根据权利要求6的涉及早期侧根形成的基因，其中所述基因编码转录因子。
8.根据权利要求7的涉及早期侧根形成的基因，其中所述转录因子选自SEQ ID N°1-SEQ ID N°19。
9.涉及早期侧根形成的转录因子，其中所述转录因子选自SEQ IDN°1-SEQ ID N°19。
10.根据权利要求9的转录因子，其中用根据权利要求1或2的方法分离编码所述转录因子的基因。
11.用根据权利要求1或2的方法分离的基因用于调节早期侧根起始的用途。
12.根据权利要求11的基因的用途，其中所述基因编码转录因子。
13.根据权利要求11或12的基因的用途，其中所述基因编码选自SEQID N°1-SEQ ID N°19的蛋白质，或者其同源物。
14.用根据权利要求3的方法分离的涉及不对称细胞分裂的基因。
15.根据权利要求14的涉及不对称细胞分裂的基因，其包含编码选自SEQ ID N°20-SEQ ID N°34的蛋白质或者其同源物的序列。
16.根据权利要求14或15的基因调节侧根形成的用途。
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05107830 | 2005-08-26 | ||
| EP05107830.1 | 2005-08-26 |
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| CN (1) | CN101287847A (zh) |
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| BR (1) | BRPI0615092A2 (zh) |
| CA (1) | CA2620267A1 (zh) |
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| AU2005319354B2 (en) | 2004-12-21 | 2012-08-30 | Monsanto Technology, Llc | Transgenic plants with enhanced agronomic traits |
| WO2007023190A2 (en) | 2005-08-26 | 2007-03-01 | Vib Vzw | Genes involved in asymmetric cell division |
| CA2669989A1 (en) | 2006-11-15 | 2008-05-22 | Agrigenetics, Inc. | Generation of plants with altered protein, fiber, or oil content |
| MX2016004595A (es) | 2013-10-09 | 2016-08-01 | Monsanto Technology Llc | Evento de maiz transgenico mon87403 y metodos para su deteccion. |
| CN105611828A (zh) | 2013-10-09 | 2016-05-25 | 孟山都技术公司 | 干扰基因表达的hd-zip转录因子抑制以产生具有增强的性状的植物 |
| US10392626B1 (en) | 2013-10-09 | 2019-08-27 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
| WO2019075409A1 (en) * | 2017-10-12 | 2019-04-18 | The Regents Of The University Of California | ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM) |
| CN108342393B (zh) * | 2018-01-25 | 2021-02-19 | 上海市农业科学院 | 一种控制水稻无侧根性状的突变基因Oslrt1、其检测及应用 |
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| US20030121070A1 (en) * | 2000-08-22 | 2003-06-26 | Luc Adam | Genes for modifying plant traits IV |
| US7345217B2 (en) * | 1998-09-22 | 2008-03-18 | Mendel Biotechnology, Inc. | Polynucleotides and polypeptides in plants |
| WO2002044337A2 (en) * | 2000-11-29 | 2002-06-06 | New York University | Wooden leg gene, promoter and uses thereof |
| US20050108791A1 (en) * | 2001-12-04 | 2005-05-19 | Edgerton Michael D. | Transgenic plants with improved phenotypes |
| BR0314389A (pt) * | 2002-09-18 | 2005-07-12 | Mendel Biotechnology Inc | Polinucleotìdeos e polipeptìdeos em plantas |
| WO2007023190A2 (en) | 2005-08-26 | 2007-03-01 | Vib Vzw | Genes involved in asymmetric cell division |
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- 2006-08-28 EP EP11155263A patent/EP2402459A3/en not_active Withdrawn
- 2006-08-28 ES ES06793029T patent/ES2390473T3/es active Active
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| WO2007023190A2 (en) | 2007-03-01 |
| ZA200802622B (en) | 2009-07-29 |
| EP1922421B1 (en) | 2012-06-27 |
| ES2390473T3 (es) | 2012-11-13 |
| US9944941B2 (en) | 2018-04-17 |
| EP2402459A3 (en) | 2012-04-04 |
| EP2402459A2 (en) | 2012-01-04 |
| CA2620267A1 (en) | 2007-03-01 |
| EP1922421A2 (en) | 2008-05-21 |
| US9102748B2 (en) | 2015-08-11 |
| AU2006283850A1 (en) | 2007-03-01 |
| AU2006283850B2 (en) | 2012-12-06 |
| BRPI0615092A2 (pt) | 2011-05-03 |
| WO2007023190A3 (en) | 2007-04-19 |
| US20150267217A1 (en) | 2015-09-24 |
| US20090144860A1 (en) | 2009-06-04 |
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