CN101285793A - Method for simultaneously determining dopamine and ascorbic acid by utilizing modified glassy carbon electrode - Google Patents
Method for simultaneously determining dopamine and ascorbic acid by utilizing modified glassy carbon electrode Download PDFInfo
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- CN101285793A CN101285793A CNA2008100363724A CN200810036372A CN101285793A CN 101285793 A CN101285793 A CN 101285793A CN A2008100363724 A CNA2008100363724 A CN A2008100363724A CN 200810036372 A CN200810036372 A CN 200810036372A CN 101285793 A CN101285793 A CN 101285793A
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Abstract
The invention relates to a method utilizing chitosan to compound hexadecylpyridine bromide to modify a glassy carbon electrode and synchronously determining dopamine and ascorbic acid, which belongs to the electrochemical analysis detection technical field. The invention is mainly to use chitosan to compound hexadecylpyridine bromide to drip on the glassy carbon electrode to modify the glassy carbon electrode to ensure that a layer of even thin-film is formed on the surface of the electrode, and further to improve the stability of the electrode. The preparation method of the compound modified agent obtained by the compounding of the chitosan and the hexadecylpyridine bromide is as follows: firstly, cetylpyridinium bromide water solution with percentage concentration by weight of between 5 and 10mM/L is prepared, then chitosan water solution with percentage concentration by weight of between 0.5 and 1.0 percent is prepared, then the cetylpyridinium bromide water solution and the chitosan water solution are mixed according to certain volume ratio, the volume ratio of the cetylpyridinium bromide water solution and the chitosan water solution is between 1:1 and 3:1, and the composite modified agent can be obtained after the mixture is stirred to fully and evenly mix. The method can be directly used for quick electrochemical determination of dopamine and ascorbic acid, and has the characteristics of quickness, sensitiveness, accuracy, high catalytic property and so on.
Description
Technical field
The present invention relates to a kind of method of utilizing the compound brocide modified glassy carbon electrode of shitosan to measure dopamine and ascorbic acid simultaneously, belong to electrochemical analysis detection technique field.
Background technology
Electrochemical method determining dopamine (DA) has caused extensive interest, is because it is important neurotransmitter, and the nervous centralis in running plays an important role in kidney and the internal system.The dopamine maladjusted nervous system is schizophrenia and Parkinsonian major reason in the brain.Therefore to the research of its assay method, to inquire into its give birth in the diagnosis of mechanism and relevant disease have great importance.Same, ascorbic acid is the vitamin in the human diet, is being used for the treatment of flu, mental disease, infertility, and cancer, acquired immune deficiency syndrome (AIDS) play an important role.Overlapped because of dopamine and ascorbic acid high overpotential and oxidation peak on bare electrode, and it can't be measured.How there is current potential optionally accurately to measure the important topic that these two kinds of materials have become Electroanalytical Chemistry, biology and medical research field.Assay method for dopamine and ascorbic acid has at present: chemoluminescence method, liquid chromatography, spectrophotometric method, fluorometry, gas chromatography-mass spectrography logotype and Capillary Electrophoresis etc.More than several assay methods to ascorbic acid and dopamine be not easy to carry, cost an arm and a leg complicated operation.
Electrochemical determination method is fast because of its speed, and cost is low, and sensitivity and accuracy advantages of higher have caused people's extensive concern.And use electrochemical method, and because of the structural formula of ascorbic acid and dopamine is close, its oxidation peak is very approaching on bare electrode and most solid electrode, because its overlapping signal exists, measuring them simultaneously becomes a big difficult point, thereby its mensuration is restricted.The galvanochemistry modified electrode can effectively reduce the overpotential of some material redox material, thereby reaches the purpose of separating and measuring.Existing bibliographical information: utilize the opposite micella effect of ascorbic acid and dopamine, these two kinds of bioactivators can be measured in cetyl trimethyl ammonium bromide (CTAB) and lauryl sodium sulfate (SDS) ionic micelle simultaneously.(two dodecyl bromination ammoniums (DDAB), and the direct modified electrode surface of anionic surfactant sodium dodecylbenzene sulfonate (SDBS), measuring ascorbic acid and dopamine simultaneously also has report to utilize cationic surfactant.But in the said method, come off easily, thereby the less stable of the modified electrode that makes with the direct modified electrode of surfactant.Shitosan is because its good bio-compatibility, easily film forming and water-insoluble, and raw material is easy to get.Utilize shitosan and surfactant to mix and to form the stabilized uniform film, can overcome the problem that simple use surfactant comes off, improved the stability of electrode to a certain extent.So far the compound brocide modified glassy carbon electrode of shitosan is not used for measuring simultaneously the bibliographical information of ascorbic acid and dopamine.
Summary of the invention
Purpose of the present invention is providing a kind of method of utilizing the compound brocide modified glassy carbon electrode of shitosan to measure dopamine and ascorbic acid simultaneously.
A kind of method of utilizing novel modified glassy carbon electrode to measure dopamine and ascorbic acid simultaneously is characterized in that having following process and step:
A. at first with glass-carbon electrode at 0.05 μ m Al
2O
3Be polished to minute surface on the polishing flannelet, then ultrasonic cleaning pre-service in distilled water, dilute nitric acid solution, absolute ethyl alcohol and redistilled water successively;
B. prepare the compound brocide composite modified glassy carbon electrode of shitosan: on the good glass-carbon electrode of pre-service, drip composite modified dose of the compound brocide of shitosan; The compound method of this dressing agent is: at first prepare bromination ten ethyl group pyridine solutions, its concentration is 5～10mM/L; Prepare chitosan aqueous solution again, its weight percent concentration is 0.5～1.0%; Then described two kinds of dressing agents are mixed according to a certain volume, both volume ratios are: chitosan aqueous solution: brocide aqueous solution=1: 1～1: 3; Stirring makes and mixes, and obtains composite modified dose;
C. a certain amount of above-mentioned composite modified dose of mixing dropped on the glass-carbon electrode matrix surface that cleans up, and at room temperature dry, put into phosphate buffered solution subsequently in pH=4.7; Then in the potential window of-0.2～0.8V cyclic voltammetry to be scanned up to figure stable; Rinse well with redistilled water again, promptly make the compound brocide modified glassy carbon electrode of shitosan:
D. above-mentioned modified glassy carbon electrode is directly used in the electrochemical gaging of dopamine and ascorbic acid; Its assay method is as follows: as working electrode, contrast electrode is a saturated calomel electrode with the compound brocide modified glassy carbon electrode of shitosan, and auxiliary electrode is the platinized platinum electrode, forms three-electrode system; This three-electrode system placed to contain certain density ascorbic acid and dopamine and pH be 4.7 phosphate buffered solution; A few carry out the differential pulse voltammetry with activated electrode to current potential afterwards from-0.2V to+0.8V cyclic voltammetry scan, and record I-E curve; Illustrate at curve map 2, on composite modified glassy carbon electrode, two independently spikes appear in ascorbic acid and dopamine, peak resolution height, and the spike potential difference reaches 0.3V; Dopamine and ascorbic acid have been realized measuring simultaneously.
The present invention utilizes shitosan excellent biological compatibility, good film forming, and and water-insoluble, fix the cationic surfactant brocide in electrode surface, thereby strengthened the stability of electrode.The compound brocide dressing agent of shitosan of the present invention can be dispersed in the surface of glass-carbon electrode, and the decorative layer of formation is more stable, helps carrying out in phosphate buffered solution voltammetric measuring.
Modified electrode among the present invention is a kind of novel electrochemical sensor, and the fast electrochemical that can be directly used in dopamine and ascorbic acid is measured, have fast, sensitive, accurately, characteristics such as catalytic height.Method of testing of the present invention has good stable and reappearance.Novel modified glassy carbon electrode its preparation method among the present invention is simple, quick, easy to operate, modifies mild condition.
Description of drawings
Fig. 1 is the dopamine and the ascorbic acid (5.0 * 10 of same concentrations among the present invention
-4M) the differential pulse voltammogram on different electrode glass carbon bare electrodes (a), the compound brocide modified glassy carbon electrode of shitosan (b).
Fig. 2 is the dopamine of variable concentrations among the present invention and the ascorbic acid oxidation peak current in the differential pulse voltammogram and the linear relationship chart of concentration on the compound brocide modified glassy carbon electrode of shitosan.。
Embodiment
After now specific embodiments of the invention being described in.
Embodiment 1
The process and the step of the assay method in the present embodiment are as follows:
(1) at first with glass-carbon electrode at 0.05 μ m Al
2O
3Be polished to minute surface on the polishing flannelet, then ultrasonic cleaning pre-service in distilled water, dilute nitric acid solution, absolute ethyl alcohol and redistilled water successively;
(2) the compound brocide composite modified glassy carbon electrode of preparation shitosan: on the good glass-carbon electrode of pre-service, drip composite modified dose of the compound brocide of shitosan; The compound method of this dressing agent is: at first prepare bromination ten ethyl group pyridine solutions, its concentration is 10mM/L; Prepare chitosan aqueous solution again, its weight percent concentration is 0.5～1.0%; Then described two kinds of dressing agents are mixed according to a certain volume, both volume ratios are: chitosan aqueous solution: brocide aqueous solution=1: 2; Stirring makes and mixes, and obtains composite modified dose;
(3) a certain amount of above-mentioned composite modified dose of mixing dropped on the glass-carbon electrode matrix surface that cleans up, and at room temperature dry, put into phosphate buffered solution subsequently in pH=4.7; Then in the potential window of-0.2～0.8V cyclic voltammetry to be scanned up to figure stable; Rinse well with redistilled water again, promptly make the compound brocide modified glassy carbon electrode of shitosan:
(4) above-mentioned modified glassy carbon electrode is directly used in the electrochemical gaging of dopamine and ascorbic acid; Its assay method is as follows: as working electrode, contrast electrode is a saturated calomel electrode with the compound brocide modified glassy carbon electrode of shitosan, and auxiliary electrode is the platinized platinum electrode, forms three-electrode system; This three-electrode system placed to contain certain density ascorbic acid and dopamine and pH be 4.7 phosphate buffered solution; A few carry out the differential pulse voltammetry with activated electrode to current potential afterwards from-0.2V to+0.8V cyclic voltammetry scan, and record I-E curve; Illustrate at curve map 2, on composite modified glassy carbon electrode, two independently spikes appear in ascorbic acid and dopamine, peak resolution height, and the spike potential difference reaches 0.3V; Dopamine and ascorbic acid have been realized measuring simultaneously.
Electrochemical gaging:
1, the dopamine of same concentrations and ascorbic acid (5.0 * 10
-4M) the differential pulse voltammetric measuring on different electrodes
Adopt different electrodes, i.e. the compound brocide modified glassy carbon electrode of glass carbon bare electrode (a) and shitosan (b).
Test condition: with the glass-carbon electrode is working electrode, is contrast electrode with the saturated calomel electrode, is auxiliary electrode with the platinized platinum electrode; The concentration of dopamine and ascorbic acid is 5.0 * 10
-4M; End liquid is the 0.1M phosphate buffered solution of pH=4.7; Take-off potential is-0.2V that the termination current potential is 0.8V; Pulse increment is 0.001; Pulse height is 0.05; Pulse width is 0.04; Be 0.2S rest time; Sensitivity is 20 * 10
-6Sweep the scope of arranging and be-0.2～+ 0.8V.
Referring to Fig. 1, from the I-E curve of Fig. 1 as seen, a broad peak only appears in the melting concn of ascorbic acid and dopamine on glass carbon bare electrode; And on composite modified glassy carbon electrode, two independently spikes appear in ascorbic acid and dopamine, peak resolution height, and the peak position difference reaches 0.3V.
2, the dopamine of variable concentrations and ascorbic acid differential pulse voltammetric measuring on composite modified glassy carbon electrode
Adopting the volumetric molar concentration of ascorbic acid is 4.0 * 10
-5(A), 1.0～10
-4(B), 2.0～10
-4(C), 3.0～10
-4(D), 4.0～10
-4(E), 5.0～10
-4(F), 6.0～10
-4(G), 7.0～10
-4(H), 8.0～10
-4M (I); Adopting the volumetric molar concentration of dopamine is the twice of ascorbic acid concentrations.
Test condition: with the composite modified glassy carbon electrode is working electrode, is contrast electrode with the saturated calomel electrode, and it becomes three-electrode system for auxiliary nest with the platinized platinum electrode; End liquid is the 0.1M phosphate buffered solution of pH=4.7.
Electricity is swept and is arranged a few with activated electrode from-0.2V to+0.8V cyclic voltammetric; Carry out the differential pulse voltammetric determination afterwards.
Referring to Fig. 2, Fig. 2 is the dopamine of variable concentrations and ascorbic acid the oxidation peak value among the differential pulse figure and the linear relationship chart of concentration on the compound brocide modified electrode of shitosan.
As can be seen from Figure 2, along with ascorbic acid and dopamine concentration increase, its oxidation peak current separately also increases.The linear relationship curve I of the bad hematic acid of hole that obtains
p=2.789C+0.488, linearly dependent coefficient r=0.9950; The dopamine linearity curve I that obtains
P=1.031C+0.035, linearly dependent coefficient r=0.9995.
Claims (1)
1. method of utilizing novel modified glassy carbon electrode to measure dopamine and ascorbic acid simultaneously is characterized in that having following process and step:
A. at first with glass-carbon electrode at 0.05 μ m Al
2O
3Be polished to minute surface on the polishing flannelet, then ultrasonic cleaning pre-service in distilled water, dilute nitric acid solution, absolute ethyl alcohol and redistilled water successively;
B. prepare the compound brocide composite modified glassy carbon electrode of shitosan: on the good glass-carbon electrode of pre-service, drip composite modified dose of the compound brocide of shitosan; The compound method of this dressing agent is: at first prepare bromination ten ethyl group pyridine solutions, its concentration is 5～10mM/L; Prepare chitosan aqueous solution again, its weight percent concentration is 0.5～1.0%; Then described two kinds of dressing agents are mixed according to a certain volume, both volume ratios are: chitosan aqueous solution: brocide aqueous solution=1: 1～1: 3; Stirring makes and mixes, and obtains composite modified dose;
C. a certain amount of above-mentioned composite modified dose of mixing dropped on the glass-carbon electrode matrix surface that cleans up, and at room temperature dry, put into phosphate buffered solution subsequently in pH=4.7; Then in the potential window of-0.2～0.8V cyclic voltammetry to be scanned up to figure stable; Rinse well with redistilled water again, promptly make the compound brocide modified glassy carbon electrode of shitosan:
D. above-mentioned modified glassy carbon electrode is directly used in the electrochemical gaging of dopamine and ascorbic acid; Its assay method is as follows: as working electrode, contrast electrode is a saturated calomel electrode with the compound brocide modified glassy carbon electrode of shitosan, and auxiliary electrode is the platinized platinum electrode, forms three-electrode system; This three-electrode system placed to contain certain density ascorbic acid and dopamine and pH be 4.7 phosphate buffered solution; A few carry out the differential pulse voltammetry with activated electrode to current potential afterwards from-0.2V to+0.8V cyclic voltammetry scan, and record I-E curve; Illustrate at curve map 2, on composite modified glassy carbon electrode, two independently spikes appear in ascorbic acid and dopamine, peak resolution height, and the spike potential difference reaches 0.3V; Dopamine and ascorbic acid have been realized measuring simultaneously.
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| RU2610220C1 (en) * | 2015-11-18 | 2017-02-08 | Федеральное государственное автономное образовательное учреждение высшего образования "Национальный исследовательский Томский государственный университет" (ТГУ, НИ ТГУ) | Method for determining ascorbic acid and dopamine in water at joint presence using modified electrodes |
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