CN101269218A - Fortifier, special bait, injection for bleeding disease immunity of Chinese idle and novel immunity method - Google Patents
Fortifier, special bait, injection for bleeding disease immunity of Chinese idle and novel immunity method Download PDFInfo
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- CN101269218A CN101269218A CNA2008100250209A CN200810025020A CN101269218A CN 101269218 A CN101269218 A CN 101269218A CN A2008100250209 A CNA2008100250209 A CN A2008100250209A CN 200810025020 A CN200810025020 A CN 200810025020A CN 101269218 A CN101269218 A CN 101269218A
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Abstract
The invention relates to grass carp hemorrhage immunizing intensifier, specific bait, injection, and new immunity method. The invention aims at overcoming the defects existing in immunity and inoculability against the main diseases in the young fry stage. The immunizing intensifier is prepared by chitosan, beta-glucan, vitamin C and vitamin E. The weaning specific bait for the grass carp fry is prepared by using the above immunizing intensifier, rotifer, vitelline, and fecula to make preparative bait, mixing the preparative bait and relevant multi-vaccine fully to make the weaning bait for grass carp fry. Adding the immunizing intensifier of the invention in the hemorrhage bacterin can promote the immunity effect; by injecting the grass carp hemorrhage bacterin and the immunizing intensifier in the abdominal cavity of the female ripe fish 30-45 days before the grass carp parents propagating for primary pre-immunity, with the specific bait which is made from fry weaning bait using the rotifer and the like to adsorb the bacterin, the immunizing and preventing effect is strengthened, and the bacterin dosage is reduced. The invention solves the problems that the operation of immunity inoculability for the grass carp at the young fry stage is difficult and the effect is poor.
Description
Technical field
The present invention relates to improve the immune effect of hemorrhagic disease of grass carp, specifically in the hemorrhagic disease of grass carp immunologic process, use the method that injects the disorders of blood vaccine in immunostimulant, specificity bait and the Ctenopharyngodon idellus parent fish body.
Background technology
The Ctenopharyngodon idellus aquaculture is along with the popularization of intensive culture pattern has obtained fast development.Yet owing to high-density breeding, throw and to raise frequency and increase, and problem such as water pollution occurs, and infects various eqpidemic disease probabilities to raise greatly, caused massive losses to aquaculture.Especially the epidemic hemorrhage outburst brings destructive strike for some regional aquacultures through regular meeting.Therefore, strengthen research, become one of China's current Ctenopharyngodon idellus aquaculture key of healthy development technology the hemorrhagic disease prevention technique.
Traditional aquatic animal diseases prevention and treatment method that is used for mainly contains two kinds, and the one, chemotherapy, the one, vaccine prevention.Though use antibiotic can partly solve problem for the moment, but antibiotic uses in a large number, can destroy the intravital microecological balance of cultivation water environment and aquatic animal, causes some pathogen to produce drug resistance, and then can cause potential threat to human beings'health again, do not meet the requirement of the Scientific Outlook on Development.Domestic immunization ways to the Ctenopharyngodon idellus seed mainly contains 3 kinds at present: injection, dipping bath and oral.(1) injection is injured because of easily causing the fish body, wastes time and energy with not easy to operate, and in the more difficult operation of fry stage, is subjected to bigger restriction; (2) dipping bath also is the normal at present a kind of approach that gives that uses, but has the technical difficult problems such as pre-treatment and immune effect difference that still need, and also is subjected to economically being widely used in producing because of the cost height is restricting; (3) though oral be a kind of approach easily, it can not be subjected to the size-dependence of time, place and aquatic animal, also can reduce labor intensity, but common vaccine is blended in general bait and easily loses, entering behind the fish body antigenicity of vaccine is subject to Digestive system and destroys, the vaccine consumption is big and immune effect is poor, is restricted on using.Traditional vaccine prevention method is for the aquatile that is grown in the water body, and the vaccinoprophylaxis operation is gone up inconvenient, and effect is also undesirable.Comprehensively above-mentioned, these three kinds of modes all exist different limitation, therefore seek the easier and effective new method of Ctenopharyngodon idellus immunity, become the research topic that needs to be resolved hurrily.
Summary of the invention
Undesirable in order to overcome present Ctenopharyngodon idellus at the defective and the immune effect of the immunity inoculation existence in seed stage, satisfy needs of production, the invention provides: the immunostimulant enhance immunity effect of using in the hemorrhagic disease of grass carp immunity; By female parent fish lumbar injection, rely on the interior antibody that produces of parent fish body to pass to prelarva, make it produce primary antibody; The specificity bait booster immunization of Ctenopharyngodon idellus Seedling oral immunity; Comprehensively use raising hemorrhagic disease of grass carp immune effect by these measures.
The technical scheme that realizes above-mentioned purpose is as follows:
The hemorrhagic disease of grass carp immunity comprises following materials of weight proportions with reinforcing agent:
Chitosan (deacetylation product) 90-100 part, beta glucan 8-10 part, vitamin C 1-3 part, vitamin E 1-3 part.
The specificity bait that is used for the hemorrhagic disease of grass carp immunity comprises following materials of weight proportions:
Reinforcing agent 2-2.5 part is used in above-mentioned hemorrhagic disease of grass carp immunity
Wheel animalcule 55-65 part
Egg yolk 28-35 part
Starch 8-10 part
Water is an amount of;
The consumption of grass carp hemorrhage disease vaccine is abideed by the requirement of production of vaccine producer, with suitable quantity of water above-mentioned preparation bait and grass carp hemorrhage disease vaccine fully is mixed into fluid and gets specificity bait, promptly as the open-mouthed bait of Ctenopharyngodon idellus Seedling.
The Ctenopharyngodon idellus bleeding disease immunity of Chinese idle is used with reinforcing agent and grass carp hemorrhage disease vaccine, the consumption of grass carp hemorrhage disease vaccine is abideed by the requirement of production of vaccine producer, the hemorrhagic disease of grass carp immunity accounts for hemorrhagic disease of grass carp vaccine injection total amount of liquid 1% with the consumption of reinforcing agent, fully mix and get injection more than 30 minutes, during preceding 30～45 days, be used for female parent fish lumbar injection in Ctenopharyngodon idellus parent breeding.
Each composition effect is as follows in the immunostimulant of the present invention:
Chitosan (deacetylation product); beta glucan can stimulate the immune system of Ctenopharyngodon idellus; and strengthen its immunocompetence; be because the partial immunity competent cell surface in the animal body exists immunostimulant (as chitosan; glucosan) receptor; when immunostimulant with after macrophage combines; the activity of immune cell activated; thereby cause such as promoting macrophage phagocytic and bactericidal activity; stimulate humoral immune reaction by complement pathway; regulate the non-specific and specific immunity process generations such as immunne response level of body immune system, can cause special antigenicity substance; the activity of nonspecific immune reaction strengthens.
Vitamin C, vitamin E can strengthen fish body immune effect.
Wheel animalcule, egg yolk and starch mix the back vaccine are had high absorption capacity, make in this way and throw something and feed, and vaccine solution loss in water body is less and more agreeable to the taste, thereby reduce the vaccine consumption.Simultaneously, they are again the agreeable to the taste bait of Ctenopharyngodon idellus Seedling.
Hemorrhagic disease of grass carp immunity of the present invention is the result who draws by optimum experimental repeatedly with the reinforcing agent prescription.
Useful technique effect of the present invention is embodied in following several aspect:
(1) in the immune protection of the popular major disease of Ctenopharyngodon idellus, use immunostimulant of the present invention oral by parent fish injection and fry, immunity has remarkable reinforced effects to Ctenopharyngodon idellus, and the existing immune level of immunoprotection force rate can improve more than 15%.
(2) select the Ctenopharyngodon idellus parent fish before artificial propagation 30～45 days, female parent fish abdominal cavity injects the grass carp hemorrhage disease vaccine, makes parent fish produce associated antibodies in vivo, and antibody vertically entails prelarva by parent, makes prelarva produce preliminary immunological memory.
(3) utilize high absorption capacity such as wheel animalcule, egg yolk and, make specificity bait absorption vaccine booster immunization effect the palatability of fry.Make in this way and throw something and feed, vaccine solution loss in water body is less and more agreeable to the taste, thereby reduces the vaccine consumption.Selecting the fry opening to carry out immunity period, is that various enzymes weaken greatly to the structural deterioration degree of vaccine, so the effect of throwing something and feeding in early days is better because the fry digestive system is not sound as yet.
(4) immunostimulant of the present invention and bait are easy to use, economical and effective.Can reduce the labor intensity of immunity inoculation, not have any bad reaction.Use immunostimulant of the present invention and bait that the Ctenopharyngodon idellus Seedling is carried out immunity, the Ctenopharyngodon idellus Seedling after the immunity reaches more than 85% the protective rate of hemorrhagic disease disease, and effective protecting time 5 months satisfies the production requirement of Ctenopharyngodon idellus seed fully.
The experiment one, the fry different times throw something and feed absorption vaccine specificity bait related experiment situation as follows:
1 materials and methods
1.1 vaccine and immunization route
The grass carp hemorrhage disease vaccine is that Zhujiang River Aquatic product institute is produced Ctenopharyngodon idellus freeze drying cell attenuated vaccine, divide 6 fry different times, from the 1st～6 week of emerging, each week is as a test group, the specificity bait test group of throwing something and feeding and the blank group of 1 contrast, in the aquarium of 100cm * 60cm * 80cm, carry out, every case is put fry 30 tails, 1d stops eating before the test, throw something and feed continuously in test specificity bait each 1 time sooner or later of every group of test fish, the 7th group is matched group, the specificity bait of not throwing something and feeding, after test finishes, put into different net cages respectively and normally raise.
1.2 serum preparation
Each gets 4 tails at random from every group of test fish, with cutting pricardial coelom blood sampling sheet, directly with the Bacillus Calmette-Gu blood sampling, in the rearmounted 4 ℃ of refrigerators of blood sampling, put take out behind the 20min centrifugal, 3000r/min, 15min draws upper serum.
1.3 agglutination
Make suspension with the tissue of hemorrhagic disease of grass carp and make antigen, the serum of experiment fish is made antibody.Serum is diluted different multiples, add 56 ℃ of water-bath 2～4h behind the abundant mixing of equal-volume virus liquid respectively, place indoor spending the night again.
1.4 protection is measured
Get the experiment fish of cage rearing respectively at random, every group 10 tail, and establish matched group and in aquarium, carry out challenge test, 1: 10 viral tissue milk 0.3mL of injected dose observes 15d continuously and adds up its mortality rate, asks protection by following formula.
Protection=(matched group death toll-oral vaccine group death toll) ÷ matched group death toll * 100%
2 results and analysis
2.1 the Ctenopharyngodon idellus Seedling of test group and matched group is raised the result relatively
Through raising in nearly 4 months, test group Ctenopharyngodon idellus Seedling is than matched group well-grown, and was no abnormal.
2.2 test group and matched group antibody agglutination titer are relatively
The Ctenopharyngodon idellus Seedling of oral bait of the present invention after raising after a while, carries out serum antibody titer to it and measures in net cage, and record at the throw something and feed Ctenopharyngodon idellus Seedling antibody agglutination titer of bait of the present invention of different times: the 1st group is 16; The 2nd～6 group is 8; Matched group is 0.Test group antibody agglutination titer is apparently higher than matched group.
2.3 test group and matched group mortality rate are relatively
Indoor measurement protection result shows; oral each group of bait test fish mortality rate of the present invention of different times is respectively 0%, 0%, 10%, 0%, 10%, 10%; the mortality rate of matched group is 100%; obtaining average protection is more than 85%; prove that oral bait of the present invention produces effect for prevention Ctenopharyngodon idellus principal disease very much, and better in the fry effect of throwing something and feeding in early days.
2.4 the test group and the matched group speed of growth are relatively
Shown in duration of test contrast and each test group gain in weight measurement result.Significant difference (t test, P＞0.05) does not appear in the gain in weight of test group fish body and the gain in weight of matched group.Explanation thus, immunostimulant does not make significant difference to the growth of Ctenopharyngodon idellus seed.
3 brief summaries and discussion
Though the digestion process of Fish is similar to high vertebrates, structure of both digestive system and function have very big difference.Cyprinid fish does not have stomach, does not have gastric gland, lacks pepsin.The digestion of intestinal replaces the digestion of stomach, and Ctenopharyngodon idellus is based on phytophage, and intestinal is short and thick; The gastral Histological research of Ctenopharyngodon idellus is thought, topmost two kinds of cells are simple columnar epithelium cell and goblet cell in the mucous layer of each section of Ctenopharyngodon idellus intestinal tube, and the specificity of digestive system structure and function is the immunostimulant of Ctenopharyngodon idellus seed stage use of the present invention and the reason that bait obtains significant curative effect.Immunostimulant that the Ctenopharyngodon idellus seed stage of the present invention uses and bait enter in the fish body through intestinal wall, stimulate the fish body to produce corresponding antibodies in vivo, and the part of these antibody is distributed to body surface by mucus.When the fish body touches this class cause of disease once more, lose appeal with the antibodies in the mucus, thereby obtain local immunity at digestive tube and body surface.The hatching digestive system is not sound as yet, and various enzymes weaken greatly to the structural deterioration degree of vaccine, so the effect of throwing something and feeding in early days is better.
Medium-height grass fry kind of the present invention uses the large-area applications situation of above-mentioned immunostimulant and special bait thereof as follows:
Be applied in the hemorrhagic disease vaccine and add immunostimulant reinforced effects of the present invention, carry out pre-immunity, fry open-mouthed bait of the present invention in preceding 30～45 days dam's fish belly chamber vaccinate of Ctenopharyngodon idellus parent fish breeding and adopt absorption vaccines such as wheel animalcule to make special bait feeding, strengthen its immunoprophylaxis effect and minimizing vaccine consumption.From 2003 so far in Hefei, Anhui Province, Chaohu, Wuhu, Chuzhou etc. the ground large tracts of land promote; produce altogether more than immune Ctenopharyngodon idellus Seedling 8,000 ten thousand tails; the Ctenopharyngodon idellus seed culture the stage to the protection of hemorrhagic disease more than 85%; effective protecting time 5 months; satisfy the production requirement of Ctenopharyngodon idellus seed fully, obtained preventive effect preferably.
Experiment two, the present invention are compared as follows with the immune hemorrhagic disease of grass carp effect of traditional oral vaccine method:
Adopt the method among the present invention, be the immunostimulant that uses in the hemorrhagic disease of grass carp immunity, the specificity bait of Ctenopharyngodon idellus Seedling oral immunity and the immunization method that passes through the female parent fish lumbar injection of Ctenopharyngodon idellus hemorrhagic disease vaccine, compare with the oral immunity effect that tradition is simple.Serum preparation, agglutination, protection method for measuring are with experiment one described method.
The result shows: not remarkable in antibody agglutination titer difference, and experiment of laboratory counteracting toxic substances and large tracts of land extend trial, the raising of hemorrhagic disease of grass carp protection exists significant difference, and average protection improves more than 15%.
Below in conjunction with embodiment the present invention is further described.
Embodiment 1:
Take off the raw material of row weight portion:
Chitosan (deacetylation product) 90 grams, beta glucan 8 grams, vitamin C 1 gram and vitamin E 1 gram, fully mix homogeneously promptly gets hemorrhagic disease of grass carp immunity reinforcing agent.
Take off the raw material of row weight portion:
The immunity of above-mentioned hemorrhagic disease of grass carp is with 8 parts of 2 parts of reinforcing agents, 60 parts of wheel animalcules, 30 parts in egg yolk and starch, with above-mentioned raw materials fully mix preparation bait; To prepare the abundant mixing and absorption of bait and grass carp hemorrhage disease vaccine above specificity bait after 15 minutes, as the open-mouthed bait of Ctenopharyngodon idellus Seedling;
The grass carp hemorrhage disease vaccine is that Zhujiang River Aquatic product institute is produced Ctenopharyngodon idellus freeze drying cell attenuated vaccine, and consumption is by manufacturer's requirement.Hemorrhagic disease of grass carp immunity is 1% of a vaccine injection total amount with the reinforcing agent consumption, fully mix 30 minutes after, bred preceding 30～45 days the Ctenopharyngodon idellus parent fish, female parent fish lumbar injection carries out pre-immunity.
Embodiment 2:
Take off the raw material of row weight portion:
Chitosan (deacetylation product) 95 grams, beta glucan 9 grams, Catergen gram and vitamin E2 gram, fully mix homogeneously promptly gets hemorrhagic disease of grass carp immunity reinforcing agent.
Take off the raw material of row weight portion:
The immunity of above-mentioned hemorrhagic disease of grass carp is with 9 parts of 2.2 parts of reinforcing agents, 55 parts of wheel animalcules, 28 parts in egg yolk and starch, with above-mentioned raw materials fully mix preparation bait; To prepare the abundant mixing and absorption of bait and grass carp hemorrhage disease vaccine above specificity bait after 15 minutes, as the open-mouthed bait of Ctenopharyngodon idellus Seedling;
Other is with embodiment 1.
Embodiment 3:
Take off the raw material of row weight portion:
Chitosan (deacetylation product) 100 grams, beta glucan 10 grams, vitamin C 3 grams and vitamin E 3 grams, fully mix homogeneously promptly gets hemorrhagic disease of grass carp immunity reinforcing agent.
Take off the raw material of row weight portion:
The immunity of above-mentioned hemorrhagic disease of grass carp is with 10 parts of 2.5 parts of reinforcing agents, 65 parts of wheel animalcules, 35 parts in egg yolk and starch, with above-mentioned raw materials fully mix preparation bait; To prepare the abundant mixing and absorption of bait and grass carp hemorrhage disease vaccine above specificity bait after 15 minutes, as the open-mouthed bait of Ctenopharyngodon idellus Seedling immunity;
Other is with embodiment 1.
Claims (3)
1, reinforcing agent is used in the hemorrhagic disease of grass carp immunity, it is characterized in that comprising the raw material of following weight portion:
Chitosan 90-100 part
Beta glucan 8-10 part
Vitamin C 1-3 part
Vitamin E 1-3 part.
2, the specificity bait that is used for the hemorrhagic disease of grass carp immunity is characterized in that comprising the raw material of following weight portion:
Reinforcing agent 2-2.5 part is used in the hemorrhagic disease of grass carp immunity
Wheel animalcule 55-65 part
Egg yolk 28-35 part
Starch 8-10 part
Water is an amount of;
With above-mentioned raw materials fully mix preparation bait, will prepare bait again with the grass carp hemorrhage disease vaccine fully mix more than 15 minutes specificity bait, the consumption of grass carp hemorrhage disease vaccine is in accordance with the requirement of manufacturer.
3, be used for injection and the novel immunity method that the Ctenopharyngodon idellus parent fish carries out pre-immunity to hemorrhagic disease, it is characterized in that comprising the raw material of following weight portion:
1 part of reinforcing agent of hemorrhagic disease of grass carp immunity
100 parts of grass carp hemorrhage disease vaccines;
With above-mentioned raw materials fully mix more than 30 minutes injection, during preceding 30～45 days, be used for female parent fish lumbar injection in the breeding of Ctenopharyngodon idellus parent fish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100250209A CN101269218A (en) | 2008-04-23 | 2008-04-23 | Fortifier, special bait, injection for bleeding disease immunity of Chinese idle and novel immunity method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008100250209A CN101269218A (en) | 2008-04-23 | 2008-04-23 | Fortifier, special bait, injection for bleeding disease immunity of Chinese idle and novel immunity method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940567A (en) * | 2010-09-21 | 2011-01-12 | 湖南康大生物药品有限责任公司 | Fish medicament for treating vitamin C and vitamin E deficiency diseases |
| CN102058884A (en) * | 2011-01-26 | 2011-05-18 | 广东海大集团股份有限公司 | Grass carp injection vaccine synergist and preparation method thereof |
| CN101810259B (en) * | 2009-12-07 | 2012-01-18 | 中国水产科学研究院淡水渔业研究中心 | Megalobrama amblycephala anti-stress feed and production method thereof |
| CN103055047A (en) * | 2012-12-04 | 2013-04-24 | 青岛绿曼生物工程有限公司 | Pure Chinese medicinal composition for treating infectious hemorrhagic disease of grass carp and preparation method thereof |
| CN103931947A (en) * | 2014-04-29 | 2014-07-23 | 临湘市东昇生物科技有限公司 | Vitaminum c (Vc)-containing fish medicine |
| CN106614154A (en) * | 2016-12-23 | 2017-05-10 | 江苏农林职业技术学院 | Method for preventing hemorrhage disease of grass carps |
-
2008
- 2008-04-23 CN CNA2008100250209A patent/CN101269218A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101810259B (en) * | 2009-12-07 | 2012-01-18 | 中国水产科学研究院淡水渔业研究中心 | Megalobrama amblycephala anti-stress feed and production method thereof |
| CN101940567A (en) * | 2010-09-21 | 2011-01-12 | 湖南康大生物药品有限责任公司 | Fish medicament for treating vitamin C and vitamin E deficiency diseases |
| CN102058884A (en) * | 2011-01-26 | 2011-05-18 | 广东海大集团股份有限公司 | Grass carp injection vaccine synergist and preparation method thereof |
| CN102058884B (en) * | 2011-01-26 | 2012-07-11 | 广东海大集团股份有限公司 | Grass carp injection vaccine synergist and preparation method thereof |
| CN103055047A (en) * | 2012-12-04 | 2013-04-24 | 青岛绿曼生物工程有限公司 | Pure Chinese medicinal composition for treating infectious hemorrhagic disease of grass carp and preparation method thereof |
| CN103055047B (en) * | 2012-12-04 | 2014-12-10 | 青岛绿曼生物工程有限公司 | Pure Chinese medicinal composition for treating infectious hemorrhagic disease of grass carp and preparation method thereof |
| CN103931947A (en) * | 2014-04-29 | 2014-07-23 | 临湘市东昇生物科技有限公司 | Vitaminum c (Vc)-containing fish medicine |
| CN106614154A (en) * | 2016-12-23 | 2017-05-10 | 江苏农林职业技术学院 | Method for preventing hemorrhage disease of grass carps |
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Open date: 20080924 |