CN101269107A - Honeysuckle flower leaf medicinal extract and extracting method - Google Patents
Honeysuckle flower leaf medicinal extract and extracting method Download PDFInfo
- Publication number
- CN101269107A CN101269107A CNA2008100549954A CN200810054995A CN101269107A CN 101269107 A CN101269107 A CN 101269107A CN A2008100549954 A CNA2008100549954 A CN A2008100549954A CN 200810054995 A CN200810054995 A CN 200810054995A CN 101269107 A CN101269107 A CN 101269107A
- Authority
- CN
- China
- Prior art keywords
- preparations
- honeysuckle
- extracting method
- leaf
- medicinal extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a medicinal extractive from honeysuckle leaves and the total content of organic acid in the extractive is 50 to 60 percent. The extract method is as follows: honeysuckle leaves are taken, cut into segments or smashed and then distilled water is added in according to the quality proportion and filtered, and then the filtrate is merged, and then the filtrate is concentrated, and hydrochloric acid is used to adjust the pH value and then centrifugated, the supernatant fluid is absorbed by a macroporous absorptive resin column and then eluted by ethanol, and then ethanol is reclaimed, dried and finally the medicinal extractive from honeysuckle leaves is obtained. The medicinal extractive from honeysuckle leaves extracted through the method together with adjuvants accepted in pharmacy can be made into clinically used preparation formulations of various types, such as tablet preparations, capsule preparations, soft capsule preparations, dispersed tablet preparations, dropping pill preparations, granule preparations, injection preparations, pilulae preparations, oral liquid preparations, subcutaneous delivery preparations and suppository preparations. The medicinal extractive from honeysuckle leaves provides a new way to make full use of honeysuckle medicinally.
Description
Technical field
The present invention relates to a kind of drug extract and extracting method thereof, particularly honeysuckle flower leaf medicinal extract and extracting method thereof.
Background technology
Flos Lonicerae (Lonicerajapoica) is one of 38 kinds of conventional Chinese medicine materials, and its effective ingredient is the organic acid composition of chlorogenic acid, has effects such as heat-clearing and toxic substances removing, wind-heat dissipating, among the people being extensive use of.Leaf of Flos Lonicerae is as a kind of by-product of Chinese medicine honeysuckle, and output is bigger, but all is dropped as non-medicinal part for a long time.Bibliographical information, the fungistatic effect of leaf of Flos Lonicerae crude extract is identical with the flower crude extract; Leaf of Flos Lonicerae has good effect for the treatment acute diarrhea.Main component also is the organic acid composition in the leaf of Flos Lonicerae, and organic acid content is about 70% in spending in its leaf, does not see bibliographical information as yet but extract high-load organic acid medicinal extract from leaf of Flos Lonicerae.Therefore, extract the higher organic acid medicinal extract of content in the leaf of Flos Lonicerae and carry out experimentation, to making full use of the Flos Lonicerae resource, significant.
Summary of the invention
The object of the present invention is to provide a kind of honeysuckle flower leaf medicinal extract.
Another object of the present invention provides the extracting method of simple, the easy to operate honeysuckle flower leaf medicinal extract of a kind of technology.
The objective of the invention is to be achieved through the following technical solutions:
Chemical constituent is an organic acid in the said honeysuckle flower leaf medicinal extract of the present invention, and total organic acids content is 50%-60%.
A kind of extracting method of honeysuckle flower leaf medicinal extract, extracting honeysuckle leaf segment or pulverizing, adding distil water boils to be carried, filter, merging filtrate, filtrate concentrates, with the hydrochloric acid adjust pH is 1-4, centrifugal, get macroporous adsorptive resins absorption on the supernatant, use ethanol elution, reclaim ethanol, merge the acid precipitation thing, drying gets honeysuckle flower leaf medicinal extract.
The adjuvant that above-mentioned honeysuckle flower leaf medicinal extract can be aided with pharmaceutics acceptance is made the dosage form of clinical normal use, as tablet, capsule, soft capsule, dispersible tablet, pill, granule, injection, drop pill, oral liquid, dosage forms such as subcutaneous administration preparation or suppository.
Said extracted method, institute's adding distil water temperature are 80 ℃-100 ℃.
The said extracted method is carried 2-4 time with the distillation decocting in water, each 0.5-2.5 hour.
Said extracted method, the amount of each institute adding distil water are 8-12 times of leaf of Flos Lonicerae quality.
The said extracted method, used alcoholic acid mass fraction is 50%-95%.
The beneficial effect that the present invention obtains is: the present invention extracts content from leaf of Flos Lonicerae be that the organic acid honeysuckle flower leaf medicinal extract of 50-60% is aided with the multiple dosage form that pharmaceutic adjuvant can be made clinical use.Extracting method of the present invention is simple, easy control of process conditions, gained medicinal extract steady quality.The present invention provides new approach for making full use of the Flos Lonicerae resource.
The specific embodiment
Following examples are used to illustrate the present invention.It is pointed out that following examples are illustrative embodiment of the present invention, but these embodiment are not construed as limiting the invention.
The extraction of embodiment 1 honeysuckle flower leaf medicinal extract
Extracting honeysuckle leaf medical material 1kg, cutting or pulverizing add 8 times of quality of distilled water of 80 ℃ at every turn and boil and carry 2 times, each 1 hour, filter merging filtrate, filtrate concentrates, and transfers pH to be 1 with hydrochloric acid, and is centrifugal, D101 macroporous adsorptive resins absorption on its supernatant, with mass fraction is 50% ethanol elution, reclaims ethanol, merges the acid precipitation thing, drying gets honeysuckle flower leaf medicinal extract 49g; Chemical constituent is an organic acid in this extract after testing, and total organic acids content is 53.5%.
The extraction of embodiment 2 honeysuckle flower leaf medicinal extracts
Extracting honeysuckle leaf medical material 1kg, cutting or pulverizing add 10 times of quality of distilled water of 100 ℃ at every turn and boil and carry 3 times, each 2 hours, filter merging filtrate, filtrate concentrates, and transfers pH to be 2 with hydrochloric acid, and is centrifugal, D101 macroporous adsorptive resins absorption on its supernatant, with mass fraction is 70% ethanol elution, reclaims ethanol, merges the acid precipitation thing, drying gets honeysuckle flower leaf medicinal extract 30g; Chemical constituent is an organic acid in this extract after testing, and total organic acids content is 51.2%
The extraction of embodiment 3 honeysuckle flower leaf medicinal extracts
Extracting honeysuckle leaf medical material 1kg, cutting or pulverizing add 12 times of quality of distilled water of 90 ℃ at every turn and boil and carry 4 times, each 2.5 hours, filter merging filtrate, filtrate concentrates and transfers pH with hydrochloric acid is 4, centrifugal, D101 macroporous adsorptive resins absorption on its supernatant, 95% ethanol elution, reclaim ethanol, merge the acid precipitation thing, drying gets honeysuckle flower leaf medicinal extract 23kg; Chemical constituent is an organic acid in this extract after testing, and total organic acids content is 50.3%
The chemical constitution of embodiment 4 honeysuckle flower leaf medicinal extracts is identified
Get the 1kg leaf of Flos Lonicerae, water extraction three times are used in chopping, and each 1 hour, be evaporated to 6 liters, the centrifuging and taking supernatant is crossed the 1kg macroporous resin.95% ethanol elution.Obtain leaf of Flos Lonicerae extract 57g behind the concentrate drying.Get extract powder 1g and separate, press chromatographic peak and collect, get compd A (40mg), B (50mg) through high speed adverse current chromatogram.
High speed adverse current chromatogram separation condition: with the solvent system chloroform: methanol: water=separate at 2: 2: 1, the detection wavelength is 254nm, flow velocity: 3ml/min, pressure: 8 kilograms.
Compd A: faint yellow amorphous powder (methanol) is chlorogenic acid through ultraviolet, infrared, mass spectrum and the contrast of high performance liquid chromatogram reference substance.
Compd B: faint yellow amorphous powder (methanol) is 3 through the chemical constitution of spectroscopic technique authenticating compound B such as ultraviolet, infrared, mass spectrum, nuclear magnetic resonance, NMR, 5-dicaffeoyl-quinic acid (3,5-Dicaffeoylquinic acid).The B molecular formula is C
25H
24O
12
Embodiment 5 honeysuckle flower leaf medicinal extract chemical constituent character are determined
It is an amount of to get extract, be configured to certain density solution with methanol, high performance liquid chromatogram with the band diode array detector detects, its main chromatographic peak ultraviolet absorption peak type unanimity, the proof leaf of Flos Lonicerae extract is same compounds, wherein 2 higher compd As, B of content are accredited as organic acid, so the leaf of Flos Lonicerae extract main component is an organic acid.Chromatographic condition: Waters high performance liquid chromatograph (auto injection, 600 pumps, 2996 ultraviolet diode array detector); 18 alkyl silica gel bonding phase packed column; Mobile phase: methanol-water (42: 58).
The reference substance solution preparation: precision takes by weighing A reference substance 2.04mg,, shakes up in the 2mL volumetric flask with methanol constant volume, and promptly getting concentration is the reference substance storing solution of 1.02mg/mL.The need testing solution preparation: extracting honeysuckle leaf macroporous resin extract 20mg, the accurate title, decide, and puts in the 25mL volumetric flask, adds methanol to scale, ultrasonic 20 minutes, shake up, filter the accurate subsequent filtrate 0.8mL that draws, put in the 25mL volumetric flask, add methanol, shake up, as need testing solution to scale.
Total organic acids assay in the leaf of Flos Lonicerae macroporous resin extract
Precision takes by weighing three batches of leaf of Flos Lonicerae macroporous resin extract samples, 2 parts every batch, every part of about 20mg, press the preparation method preparation of need testing solution after, measure trap at 327nm place, calculating total organic acids content.
The results are shown in following table 1.
Table 1 leaf of Flos Lonicerae macroporous resin extract sample determination result
As calculated, the average content of total organic acids is 52.71% in the leaf of Flos Lonicerae extract.
Claims (6)
1, a kind of honeysuckle flower leaf medicinal extract is characterized in that its total organic acids content is 50%-60%.
2, a kind of extracting method of honeysuckle flower leaf medicinal extract is characterized in that: extracting honeysuckle leaf segment or pulverizing, adding distil water are boiled and are carried, filter, merging filtrate, filtrate concentrates, with the hydrochloric acid adjust pH is 1-4, centrifugal, get macroporous adsorptive resins absorption on the supernatant, use ethanol elution, reclaim ethanol, merge the acid precipitation thing, drying gets honeysuckle flower leaf medicinal extract.
3, extracting method according to claim 2 is characterized in that: institute's adding distil water temperature is 80 ℃-100 ℃.
4, according to claim 2 or 3 described extracting method, it is characterized in that: adding distil water boils to be carried 2-4 time, each 0.5-2.5 hour.
5, extracting method according to claim 4 is characterized in that: the amount of each institute adding distil water is 8-12 a times of leaf of Flos Lonicerae quality.
6, extracting method according to claim 2 is characterized in that: used alcoholic acid mass fraction is 50%-95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100549954A CN101269107A (en) | 2008-05-20 | 2008-05-20 | Honeysuckle flower leaf medicinal extract and extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100549954A CN101269107A (en) | 2008-05-20 | 2008-05-20 | Honeysuckle flower leaf medicinal extract and extracting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101269107A true CN101269107A (en) | 2008-09-24 |
Family
ID=40003498
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100549954A Pending CN101269107A (en) | 2008-05-20 | 2008-05-20 | Honeysuckle flower leaf medicinal extract and extracting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101269107A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785568A (en) * | 2010-03-24 | 2010-07-28 | 牟一心 | Honeysuckle leaf distillate beverage and preparation method thereof |
| CN102988457A (en) * | 2012-12-24 | 2013-03-27 | 重庆市中药研究院 | Total flavone extract of lonicera macranthoides leaves, and preparation method and application thereof |
| CN103830306A (en) * | 2014-02-20 | 2014-06-04 | 鲁南制药集团股份有限公司 | Preparation method of honey suckle leaf effective extract product |
| CN104027401A (en) * | 2014-07-07 | 2014-09-10 | 河南太龙药业股份有限公司 | Shuanghuanglian medicine combination used for virus resisting, heat clearing and inflammation diminishing of children and preparing method thereof |
| CN108785358A (en) * | 2018-08-27 | 2018-11-13 | 维康腾达生物科技有限公司 | A kind of preparation method of Honegsukle flower P.E |
| CN112546079A (en) * | 2021-01-28 | 2021-03-26 | 广西壮族自治区中医药研究院 | Preparation and quality detection method of total organic acid extract in Chinese fir leaves |
| CN113181232A (en) * | 2021-05-27 | 2021-07-30 | 山东省科学院菏泽分院 | Substance for treating acute pancreatitis |
-
2008
- 2008-05-20 CN CNA2008100549954A patent/CN101269107A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785568A (en) * | 2010-03-24 | 2010-07-28 | 牟一心 | Honeysuckle leaf distillate beverage and preparation method thereof |
| CN102988457A (en) * | 2012-12-24 | 2013-03-27 | 重庆市中药研究院 | Total flavone extract of lonicera macranthoides leaves, and preparation method and application thereof |
| CN103830306A (en) * | 2014-02-20 | 2014-06-04 | 鲁南制药集团股份有限公司 | Preparation method of honey suckle leaf effective extract product |
| CN103830306B (en) * | 2014-02-20 | 2017-08-25 | 鲁南制药集团股份有限公司 | A kind of preparation method of folium lonicerae effective extract |
| CN104027401A (en) * | 2014-07-07 | 2014-09-10 | 河南太龙药业股份有限公司 | Shuanghuanglian medicine combination used for virus resisting, heat clearing and inflammation diminishing of children and preparing method thereof |
| CN104027401B (en) * | 2014-07-07 | 2016-08-17 | 河南太龙药业股份有限公司 | A kind of for child's antiviral, the SHUANGHUANLIAN drug regimen and preparation method thereof of heat clearing and inflammation relieving |
| CN108785358A (en) * | 2018-08-27 | 2018-11-13 | 维康腾达生物科技有限公司 | A kind of preparation method of Honegsukle flower P.E |
| CN112546079A (en) * | 2021-01-28 | 2021-03-26 | 广西壮族自治区中医药研究院 | Preparation and quality detection method of total organic acid extract in Chinese fir leaves |
| CN113181232A (en) * | 2021-05-27 | 2021-07-30 | 山东省科学院菏泽分院 | Substance for treating acute pancreatitis |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101269107A (en) | Honeysuckle flower leaf medicinal extract and extracting method | |
| CN101411745B (en) | Antibiotic tanshinone extract, preparation method thereof, use and products produced thereby | |
| CN1895408B (en) | Radix saposhnikoviae Tong Sheng capsules, preparation and quality control method thereof | |
| CN103823034B (en) | A kind of honeysuckle reference extract and preparation method thereof | |
| CN103463531B (en) | Use of rhizoma smilacis glabrae extract in preparation of lipid-lowering and fat-reducing medicines and health-care foods | |
| CN101343225A (en) | Preparation method for high-purity di-coffee mesitoyl quinine acid compounds | |
| CN102731586A (en) | Preparation technology of stilbene glucoside in polygonum multiflorum | |
| CN101313927A (en) | Southernwood total flavone, method for preparing its composition and medicine uses thereof | |
| CN101757099B (en) | Desmodium-capillary artemisia cholecystagogue, preparation method and quality control method thereof | |
| CN101401829A (en) | Wild Jinchai liveness extract, preparation and uses thereof | |
| US11571453B2 (en) | Piper laetispicum extract and preparation method therefor and use thereof | |
| CN102872015B (en) | Stephanotis total alkaloid extract as well as preparation method and application thereof | |
| CN102702283B (en) | Method for quickly separating and preparing high-purity deoxyrhapontin and rhapontin | |
| CN101690757B (en) | Method for preparing bezoar supernatant preparations | |
| CN100525784C (en) | Laggera pterodonta buccal tablet, its preparation process and quality control method | |
| CN100515435C (en) | Extracting method and use of ainsliaea fragrans champ-extract | |
| CN110194758B (en) | Method for separating and purifying aristolochic acid compounds from caulis Aristolochiae Manshuriensis | |
| CN100453080C (en) | Lindera root alkaloid, its preparation method and application in medicine preparation | |
| CN109053756B (en) | Phenylpropanoid ester type catechin and preparation method and application thereof | |
| CN107721857B (en) | Method for preparing high-purity chlorogenic acid from gynura procumbens | |
| CN103142474B (en) | With the composition and method of making the same that high purity bilobalide B is active component | |
| CN107266464B (en) | A kind of rhizoma alismatis decoction extract and its application | |
| CN1788748B (en) | Detection method of injection formulation containing raw material herb red sage root or red sage | |
| Liang et al. | Determination of iridoid glucosides for quality assessment of Herba Oldenlandiae by high-performance liquid chromatography | |
| CN103012510A (en) | Preparation method of 1,2,3,4,6-pentagalloylglucose reference substances |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080924 |