CN101260378A - Application of rhodococcus erythropolis in cleaning heavy oil dirt - Google Patents
Application of rhodococcus erythropolis in cleaning heavy oil dirt Download PDFInfo
- Publication number
- CN101260378A CN101260378A CNA2008100940488A CN200810094048A CN101260378A CN 101260378 A CN101260378 A CN 101260378A CN A2008100940488 A CNA2008100940488 A CN A2008100940488A CN 200810094048 A CN200810094048 A CN 200810094048A CN 101260378 A CN101260378 A CN 101260378A
- Authority
- CN
- China
- Prior art keywords
- heavy oil
- rhodococcus erythropolis
- cleaning
- biological cleaner
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000295 fuel oil Substances 0.000 title claims abstract description 54
- 241000187561 Rhodococcus erythropolis Species 0.000 title claims abstract description 31
- 238000004140 cleaning Methods 0.000 title claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 210000001082 somatic cell Anatomy 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 5
- 239000011521 glass Substances 0.000 abstract description 14
- 238000002360 preparation method Methods 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000012459 cleaning agent Substances 0.000 abstract description 4
- 239000010779 crude oil Substances 0.000 abstract description 4
- 239000002184 metal Substances 0.000 abstract description 4
- 229910052751 metal Inorganic materials 0.000 abstract description 4
- 239000004033 plastic Substances 0.000 abstract description 4
- 229920003023 plastic Polymers 0.000 abstract description 4
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 238000005238 degreasing Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract 1
- 150000002739 metals Chemical class 0.000 abstract 1
- 238000005065 mining Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 14
- 239000003921 oil Substances 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 6
- 239000007921 spray Substances 0.000 description 6
- 241000817821 Rhodococcus erythropolis XP Species 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 238000006477 desulfuration reaction Methods 0.000 description 4
- 230000023556 desulfurization Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000002985 plastic film Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229940051841 polyoxyethylene ether Drugs 0.000 description 1
- 229920000056 polyoxyethylene ether Polymers 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 229960004418 trolamine Drugs 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
Abstract
The invention discloses a novel function of Rhodococcus erythropolis in cleaning of heavy oil scales, wherein, heavy oil biological cleaning agent can be prepared by utilization of thallus cells generated by the Rhodococcus erythropolis for preparation of bacterial solution with water. Particularly, the heavy oil biological cleaning agent consists of thallus cells XP which are cultured by Rhodococcus erythropolis strain CCTCC M 205068 and water, wherein, the dry weight concentration of the Rhodococcus erythropolis thallus cells XP is 8 to 20 grams per liter and the optimized concentration is 12 to 15 grams per liter. Compared with the prior art, the novel function of the Rhodococcus erythropolis in the cleaning of the heavy oil scales has the advantages of good use effect, strong degreasing ability, small environmental pollution and so on, can clean the heavy oil scales on surfaces of metals, plastics, glass and so on and can be widely used for cleaning work platforms, machinery equipment, components and parts, containers and pipes in crude oil mining and production places.
Description
Technical field
The present invention relates to microorganism field, the particularly known new application of rhodococcus erythropolis (Rhodococcus erythropolis) in cleaning heavy oil dirt with oil desulfurization function.
Background technology
Rhodococcus erythropolis (Rhodococcus erythropolis) CCTCC M 205068 is bacterial strains that a strain that the contriver filters out in one's early years has the oil desulfurization function; bacterial strain is preserved in (address: Chinese Wuhan City, Hubei Province Wuhan University, Chinese typical culture collection center on June 20th, 2005; postcode 430072); Chinese patent 200510044599.X has obtained the patent protection of this bacterial strain, and describes the obtaining and using of screening, culture of strains and bacterium liquid of this bacterial strain in this patent in detail.Previous patent and document only rest on it aspect the oil desulfurization to the research and the utilization of this bacterial strain, do not find its other functions.
In the Oil extraction field, all can produce heavy oil dirt in the process systems such as workplatform, machinery equipment, container, pipeline, in Crude Oil Processing, also can in processing units, component and container, produce greasy dirt.Cleaning this type of smeary clean-out system mostly is chemicals, contains alkaline matter and/or organic solvent, and certain corrodibility is arranged.Chinese patent application 200610013444.4 discloses a kind of heavy oil cleaning agent, by weight percentage by tetrasodium ethylenediamine tetraacetate 1~5; Sodium Nitrite 0.1~1; Yellow soda ash 0.5~2; Fatty alcohol-polyoxyethylene ether 2~10; Polyoxyethylenated alcohol sodium sulfate 1~5; Trolamine 0.2~1 and excess water are formulated, though its alkali-free material, but because of wherein containing a variety of inorganic salt and organic salt, remaining in being discharged in the waste liquid after the cleaning can not be degraded in a short time, still there is pollution, can not satisfies the high request of recycling economy and environmental protection environment.Therefore the heavy oil cleaning agent of seeking a kind of high-efficiency environment friendly is still an industry difficult problem.
Summary of the invention
Purpose of the present invention proposes the new purposes of rhodococcus erythropolis (Rhodococcus erythropolis) in cleaning heavy oil dirt.
The somatic cells water that the present invention utilizes rhodococcus erythropolis (Rhodococcus erythropolis) to produce is made into bacterium liquid, promptly can be made into a kind of heavy oil biological cleaner.
Concrete, the heavy oil biological cleaner during the present invention uses is made up of somatic cells XP and water that rhodococcus erythropolis (Rhodococcuserythropolis) bacterial strain CCTCC M 205068 cultivates.Wherein, rhodococcus erythropolis somatic cells XP dry weight concentrations is 8-20g/L; More the concentration of You Huaing is 12-15g/L.
Use this biological cleaner to clean the heavy oil greasy dirt, can overcome the deficiency of existing chemical, have efficiently remove contamination, non-environmental-pollution, economical and practical characteristics.This biological cleaner also has a main advantages, behind the on-the-spot greasy dirt that uses in this biological cleaner removal process system of crude oil extraction, cleaning waste liquid can discharge on the spot, can back to nature with the bacterium that waste liquid infiltrates in soil or the oil reservoir, can not pollute environment, and, utilize its desulfurization characteristic also can reduce sulphur content in the crude oil, therefore not only environmental protection of this biological cleaner also meets the theory of recycling economy, is an important breakthrough to the multiple application of microorganism.
Advantages such as it is good that the present invention compared with prior art has result of use, and degreasing power is strong, and environmental pollution is little.Heavy oil dirt in surface such as the present invention can clean metal, plastic and glass and container, the pipeline is applicable to the cleaning to workplatform, machinery equipment and component in Oil extraction and the production place, container, pipeline.
Embodiment
The present invention has mainly proposed the new purposes of rhodococcus erythropolis (Rhodococcus erythropolis) in cleaning heavy oil dirt.
Used rhodococcus erythropolis among the present invention (Rhodococcus erythropolis), be that to be preserved in Chinese typical culture collection center (address: Chinese Wuhan City, Hubei Province Wuhan University, postcode 430072) preserving number be the strain bacterium of CCTCC M 205068.The detailed process of obtaining of the selection of bacterial strain, culture of strains and somatic cells sees also the detailed introduction of granted patent 200510044599.X, the present invention below describes the somatic cells called after XP cell of the rhodococcus erythropolis of acquisition the new application of this XP cell in detail.
The present invention directly is hybridly prepared into the certain density aqueous solution with water with the XP cell, and this aqueous solution promptly forms the heavy oil biological cleaner and can be used for the cleaning of heavy oil smeary.Further specify below in conjunction with embodiment and cleaning experiment:
Embodiment 1: the preparation of heavy oil biological cleaner A1
The rhodococcus erythropolis XP cell of 8 gram dry weights is suspended with 1 premium on currency, can use after stirring.
Embodiment 2: the preparation of heavy oil biological cleaner A2
The rhodococcus erythropolis XP cell of 20 gram dry weights is suspended with 1.5 premium on currency, can use after stirring.
Embodiment 3: the preparation of heavy oil biological cleaner A3
The rhodococcus erythropolis XP cell of 10 gram dry weights is suspended with 1.0 premium on currency, can use after stirring.
Embodiment 4: the preparation of heavy oil biological cleaner A4
The rhodococcus erythropolis XP cell of 300 gram dry weights is suspended with 20.0 premium on currency, can use after stirring.
Embodiment 5: the preparation of heavy oil biological cleaner A5
The rhodococcus erythropolis XP cell of 20 gram dry weights is suspended with 1.0 premium on currency, can use after stirring.
Experimental example 1: heavy oil biological cleaner A2 is to the cleaning of glass surface heavy oil
Sample: area is 26.5 square centimeters a glass, and there is heavy oil greasy dirt 0.41 gram on the surface, and greasy dirt is applied on glass surface;
Experimental technique: with the XP cell concn be 20 milliliters of sprays of biological cleaner (A2) of 13 grams per liters at sample surfaces, leave standstill 20s, dash with the about 30ml of clear water again and drench; Observe the greasy dirt adhesion condition of glass surface before and after cleaning;
Experimental result: after using the biological cleaner spray, observe oil stain on glass surface and scatter rapidly, after pouring, oil stain on glass surface is removed fully with clear water.
Experimental example 2: heavy oil biological cleaner A1 is to the cleaning of metallic surface heavy oil
Sample: area is 40 square centimeters a stainless-steel sheet, and the surface scribbles heavy oil greasy dirt 0.96 gram;
Experimental technique: get 20 milliliters of the biological cleaners that the XP cell concn is 8 grams per liters (A1), gradation sprays at sample surfaces, leaves standstill 20s behind each spray, holds up sample and makes under the liquid flow, repeats several times; Observe the greasy dirt adhesion condition of metallic surface before and after cleaning;
Result: after using the biological cleaner spray, observe oil stain on glass surface and scatter gradually, repeatedly behind the spray, hold up sample, observe the greasy dirt of metallic surface after under the liquid flow and removed fully.
Experimental example 3: heavy oil biological cleaner A3 is to the cleaning of frosting heavy oil
Sample: area is 8 square centimeters a plastic sheet, and the two sides all scribbles heavy oil greasy dirt totally 1.2 grams;
Experimental technique: with the XP cell concn is that the biological cleaner (A3) of 10 grams per liters is put into glass beaker for 20 milliliters, and the sample input is wherein soaked the about 30s of concussion, takes out observation;
The result: the plastic sheet two sides greasy dirt of taking-up is removed fully.
Biological cleaner provided by the invention is used in above experimental example explanation, can effectively remove the heavy oil dirt on surfaces such as metal, plastic and glass; Prompting can use this biological cleaner to clean machinery equipment, piece surface and the service platform that is stained with the various materials of heavy oil smeary.
Experimental example 4: heavy oil biological cleaner A5 is to the cleaning of heavy oil in the Glass Containers
Sample: hold the 500ml Erlenmeyer flask of heavy oil sample, after heavy oil was poured out, its inwall hung with about 10 grams of thick and heavy heavy oil greasy dirt;
Experimental technique: with the XP cell concn is that the biological cleaner (A5) of 20 grams per liters is poured in the Erlenmeyer flask for 3 liters in batches, and all the rotation conic bottle makes a solution contact bottle wall at every turn, pours out wherein solution behind the 30s, uses the 200ml flushing with clean water at last again.
The result: the full scale clearance of Erlenmeyer flask inwall heavy oil, Glass Containers is limpid again transparent.
Biological cleaner provided by the invention is used in this experimental example prompting, can effectively remove adherent heavy oil greasy dirt in the container.
Experimental example 5: heavy oil biological cleaner A4 cleans heavy oil smeary in the pipeline
Sample: the retired pipeline 0.9m that scraps of oil recovery factory's dismounting, diameter is 2.54 centimetres, because long service, inner-walls of duct retains thicker heavy oil greasy dirt; Weight 9.2kg before the record sample cleans;
Experimental technique: the XP cell concn is that biological cleaner (A4) 5 liftings of 15 grams per liters place container, use a pump to extract detergent solution from container, pump line is communicated with sample pipe, make detergent solution circulation flushing inner-walls of duct, flow velocity is 1.5 liters/minute, wash and stop after 10 minutes, sample pipe is taken out dry; Observing the inner-walls of duct situation also weighs once more;
The result: can't see inwall from the sample pipe two ends after cleaning and have tangible greasy dirt, cleaning the back weighing results is 9kg, has alleviated 0.2kg before cleaning, and shows that greasy dirt is eliminated in the pipeline of cleaning back.
Biological cleaner provided by the invention is used in this experimental example prompting, can effectively remove adherent heavy oil greasy dirt in the pipeline, and pipeline in being on active service is carried out periodic flushing, can prolong the duration of service of pipeline.
More than illustrated that by example biological cleaner of the present invention can effectively remove the heavy oil dirt in surface such as metal, plastic and glass and container, the pipeline, after testing, before and after cleaning, cellular constituent of clean-out system own and counting all do not change, the heavy oil component and the consumption that are eliminated do not change yet, and this shows that be a kind of physical action with heavy oil by removing from various surfaces by biological cleaner.Therefore, the heavy oil and the cell that still contain original state in the waste liquid after having cleaned, and total amount is preceding consistent with cleaning, having pointed out use biological cleaner of the present invention to remove the smeary advantage is, the making of clean-out system and use can not introduced new pollution, and compare, chemical will be introduced the soda acid pollution or other organic solvent pollutes.
Claims (5)
1, the application of rhodococcus erythropolis (Rhodococcus erythropolis) in cleaning heavy oil dirt.
2, according to the described application of claim 1, it is characterized in that, will be made into bacterium liquid, make a kind of heavy oil biological cleaner by the somatic cells water that described rhodococcus erythropolis (Rhodococcus erythropolis) produces.
According to the described application of claim 2, it is characterized in that 3, rhodococcus erythropolis somatic cells dry weight concentrations is 8-20g/L in the described heavy oil biological cleaner.
According to the described application of claim 2, it is characterized in that 4, rhodococcus erythropolis somatic cells dry weight concentrations is 12-15g/L in the described heavy oil biological cleaner.
According to the arbitrary described application of claim 1 to 4, it is characterized in that 5, described rhodococcus erythropolis (Rhodococcus erythropolis) is the bacterial strain of CCTCCM 205068 for being preserved in Chinese typical culture collection center preserving number.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2008100940488A CN101260378B (en) | 2008-04-28 | 2008-04-28 | Application of rhodococcus erythropolis in cleaning heavy oil dirt |
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CN2008100940488A CN101260378B (en) | 2008-04-28 | 2008-04-28 | Application of rhodococcus erythropolis in cleaning heavy oil dirt |
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CN101260378A true CN101260378A (en) | 2008-09-10 |
CN101260378B CN101260378B (en) | 2011-02-02 |
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CN2008100940488A Expired - Fee Related CN101260378B (en) | 2008-04-28 | 2008-04-28 | Application of rhodococcus erythropolis in cleaning heavy oil dirt |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101974433A (en) * | 2010-10-21 | 2011-02-16 | 西北农林科技大学 | Carbendazol composite degrading bacteria and method for degrading carbendazol |
CN103668238A (en) * | 2013-12-31 | 2014-03-26 | 张锦碧 | Environment-friendly microorganism degreasing agent |
CN107099388A (en) * | 2017-05-23 | 2017-08-29 | 芜湖欧标农业发展有限公司 | A kind of high-purity Chinese honey locust total saposins extracting method |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1323160C (en) * | 2005-09-14 | 2007-06-27 | 山东大学 | Rhodococcus erythropolis and its uses in crude oil desulfuration |
-
2008
- 2008-04-28 CN CN2008100940488A patent/CN101260378B/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101974433A (en) * | 2010-10-21 | 2011-02-16 | 西北农林科技大学 | Carbendazol composite degrading bacteria and method for degrading carbendazol |
CN103668238A (en) * | 2013-12-31 | 2014-03-26 | 张锦碧 | Environment-friendly microorganism degreasing agent |
CN107099388A (en) * | 2017-05-23 | 2017-08-29 | 芜湖欧标农业发展有限公司 | A kind of high-purity Chinese honey locust total saposins extracting method |
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Publication number | Publication date |
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CN101260378B (en) | 2011-02-02 |
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