CN101249128A - Preparation of barren wort total chromocor - Google Patents
Preparation of barren wort total chromocor Download PDFInfo
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- CN101249128A CN101249128A CNA2008100271120A CN200810027112A CN101249128A CN 101249128 A CN101249128 A CN 101249128A CN A2008100271120 A CNA2008100271120 A CN A2008100271120A CN 200810027112 A CN200810027112 A CN 200810027112A CN 101249128 A CN101249128 A CN 101249128A
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- herba epimedii
- total flavones
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Abstract
The invention discloses a preparation method for total flavone of barrenwort, which comprises the following steps: coarse powder of barrenwort leaves is obtained and is refluxed and extracted for 1 to 3 times by using ethanol of 50 to 70 percent which is 8 to 12 times the quantity of the coarse, and the time for each extraction is 1 to 2 hours; the filtering is conducted; the filter liquor is decompressed and concentrated to a clear paste after being merged, wherein, the relative density of the clear paste is 1.05 to 1.10; then the clear paste is centrifugated at 5000r/min high speed and spray-dried, so that the total flavone extract of the barrenwort is obtained. The preparation method has the advantages of higher yield, simpleness and easy operation, shortens the time and the procedure of machining operation, and is easy to form industrialized production operation.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicine pharmacy, particularly a kind of preparation method of Herba Epimedii total flavones.
Background technology
Herba Epimedii is China's tradition kidney invigorating and YANG supporting Chinese medicine, and the dry aerial parts that are divided into Berberidaceae plant Herba Epimedii (Epimediumbrevicornum Maxim), arrow leaf Herba Epimedii (Epimedium sagittatum Maxim), pubescence Herba Epimedii (Epimedium pubescens Maxim), Epimedium wushanense (Epimedium wushanenseT.S.Ying) or Herba Epimedii (Epimedium koreanum Nakai) are used as medicine.Herba Epimedii has kidney-replenishing, bone and muscle strengthening, and the effect of wind-damp dispelling is generally used for impotence and seminal emission, and the muscles and bones flaccidity is soft, rheumatic arthralgia, numb detention; Climacteric hypertension etc.The composition of Herba Epimedii performance effect is mainly its total flavones and polysaccharide compound.
To Herba Epimedii total flavones preparation method more, as water extract-alcohol precipitation, ethanol extraction, n-butanol extraction and water extraction, absorption with macroporous adsorbent resin separate or the like.Separating the preparation total flavones at present adopts macroporous resin to prepare more, but when going up macroporous resin owing to the concentrated back of extracting solution, its volume is relatively large, and viscosity is also bigger, operation inconvenience during last macroporous resin, cause the absorption of pillar during simultaneously spissated extractum upper prop easily, can not parallel eluting when causing eluting, delay longer when pillar outward appearance colour band inequality, eluting.
Summary of the invention
The present invention is directed to existing Herba Epimedii total flavones preparation and have problems such as operating time length, program complexity, a kind of preparation method of Herba Epimedii total flavones is provided.
The present invention can be achieved by the following technical programs: a kind of preparation method of Herba Epimedii total flavones: get the Folium Epimedii coarse powder, use alcohol reflux, filter, filtrate merges the back concentrating under reduced pressure and becomes clear paste, behind the 5000r/min high speed centrifugation, carry out spray drying again, get the Herba Epimedii total flavones extract.
In above-mentioned preparation method, described ethanol consumption is 8～12 times, and its concentration is 60～70%; Described extraction time is 1～3 time, and each time is 1～2h; The relative concentration of described clear paste is 1.05～1.10.
Compared with prior art, the present invention has the following advantages: preparation method yield of the present invention is higher, and simple easy operating, shortens process operation time and program, forms the suitability for industrialized production operation easily.
The specific embodiment
Below in conjunction with embodiment the specific embodiment of the present invention is described in detail.
Adopt in the process of the present invention, high spot reviews the reasonability and the feasibility of technology.Because mostly the traditional extraction process of flavone is to adopt alcohol extraction, therefore to Herba Epimedii coarse powder ethanol extraction, is the investigation factor with concentration of alcohol, ethanol consumption and extraction time and extraction time, adopts L
9(3
4) orthogonal test table is tested, and serves as to investigate index with the Herba Epimedii total flavones rate of transform, the icariin rate of transform, determines the best of breed of Herba Epimedii coarse powder ethanol extraction.
Get Herba Epimedii coarse powder 100g, put in the 2500ml round-bottomed flask, in the constant temperature electric heating cover, scheme by Orthogonal Experiment and Design is carried out reflux, extract,, filters reclaim under reduced pressure and to be concentrated into relative concentration be 1.05～1.10 thick paste shape, vacuum drying is to constant weight, measure receipts cream rate, Herba Epimedii total flavones and the content Determination of Icariin of dried cream respectively, and calculate its rate of transform, its result of the test sees Table 1,2.
Table 1 orthogonal test factor level table
Table 2 reflux, extract, condition orthogonal test table
Herba Epimedii total flavones content in the Herba Epimedii total flavones rate of transform (%)=(the dried cream yield of general flavone content * in the dried cream)/medical material
Icariin content in the icariin rate of transform (%)=(the dried cream yield of sheep icariin content * during dried cream is excessive)/medical material
* overall merit=Herba Epimedii total flavones rate of transform * 50%+ icariin the rate of transform * 50%
Investigate with comprehensive evaluation index behind the ethanol extraction, show that by extreme difference R value size in the table 2 each factor affecting size is respectively A＞D＞B＞C; Table 2 The results of analysis of variance shows: there are no significant the influence (P＞0.05) of B, C, D three factors, the A factor has significance influence (P＜0.01), so with A
2B
2C
3D
1Be optimised process, promptly with 10 times of amount ethanol extractions of 60% 3 times, each 2h.
With the extracting solution that the optimum extraction process of investigating obtains, decompression recycling ethanol also is concentrated into relative density 1.05-1.10 (50 ℃), high speed centrifugation (5000r/min), and the supernatant water-bath concentrates, and vacuum drying is to constant weight.Measure respectively and receive cream rate, total flavones and icariin content.Result of the test sees Table 3:
The centrifugal back of table 3 total flavones is investigated table as a result
Result of the test shows: concentrated solution is centrifugal through 5000r/min, and the total flavones rate of transform and the icariin rate of transform are all higher in the dried cream.
Whole test result shows, after Herba Epimedii adopted the solution concentration that ethanol extraction obtains, its extractum was centrifugal, obtains thick total flavones after the supernatant drying, and the rate of transform of its total flavones is nearly 80%, and icariin content reaches about 4.6%.Whole test is simple to operate, is easy to suitability for industrialized production.The Herba Epimedii total flavones crude extract that obtains can be made various dosage forms, as hard capsule, tablet, granule, drop pill, oral liquid etc., preferably capsule.
Adopt method of the present invention, its total flavones rate of transform of the product that makes behind the total flavones high speed centrifugation that obtains is nearly 80%, and the total flavones yield is about 5.4% of crude drug.It is simple that this invention focuses on process, and yield is higher, is easy to the big production of industrialization.
Claims (5)
1. the preparation method of a Herba Epimedii total flavones is characterized in that: get the Folium Epimedii coarse powder, use alcohol reflux, filter, filtrate merges the back concentrating under reduced pressure and becomes clear paste, carries out spray drying again behind the 5000r/min high speed centrifugation, gets the Herba Epimedii total flavones extract.
2. preparation method according to claim 1 is characterized in that: described ethanol consumption is 8～12 times.
3. preparation method according to claim 2 is characterized in that: described concentration of ethanol is 60～70%.
4. preparation method according to claim 3 is characterized in that: described extraction time is 1～3 time, and each time is 1～2h.
5. preparation method according to claim 4 is characterized in that: the relative concentration of described clear paste is 1.05～1.10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100271120A CN101249128A (en) | 2008-03-31 | 2008-03-31 | Preparation of barren wort total chromocor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100271120A CN101249128A (en) | 2008-03-31 | 2008-03-31 | Preparation of barren wort total chromocor |
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| Publication Number | Publication Date |
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| CN101249128A true CN101249128A (en) | 2008-08-27 |
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| CNA2008100271120A Pending CN101249128A (en) | 2008-03-31 | 2008-03-31 | Preparation of barren wort total chromocor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104000862A (en) * | 2014-06-11 | 2014-08-27 | 南方医科大学 | Application of total flavonoids of herba epimedii in preparing medicine for preventing hepatic fibrosis |
-
2008
- 2008-03-31 CN CNA2008100271120A patent/CN101249128A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104000862A (en) * | 2014-06-11 | 2014-08-27 | 南方医科大学 | Application of total flavonoids of herba epimedii in preparing medicine for preventing hepatic fibrosis |
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Open date: 20080827 |