CN101246171A - Portable hepatitis B fast joint inspection device - Google Patents
Portable hepatitis B fast joint inspection device Download PDFInfo
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- CN101246171A CN101246171A CNA2008100452836A CN200810045283A CN101246171A CN 101246171 A CN101246171 A CN 101246171A CN A2008100452836 A CNA2008100452836 A CN A2008100452836A CN 200810045283 A CN200810045283 A CN 200810045283A CN 101246171 A CN101246171 A CN 101246171A
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- test strips
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- hbsag
- hbeag
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- 238000007689 inspection Methods 0.000 title claims description 42
- 208000002672 hepatitis B Diseases 0.000 title claims description 23
- 238000012360 testing method Methods 0.000 claims abstract description 193
- 238000001514 detection method Methods 0.000 claims description 72
- 101710142246 External core antigen Proteins 0.000 claims description 38
- 210000004369 blood Anatomy 0.000 claims description 33
- 239000008280 blood Substances 0.000 claims description 33
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 33
- 239000010931 gold Substances 0.000 claims description 33
- 229910052737 gold Inorganic materials 0.000 claims description 33
- 239000000084 colloidal system Substances 0.000 claims description 14
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 10
- 241001494479 Pecora Species 0.000 claims description 10
- 238000003908 quality control method Methods 0.000 claims description 10
- 101710132601 Capsid protein Proteins 0.000 claims description 3
- 230000037213 diet Effects 0.000 claims description 2
- 235000005911 diet Nutrition 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 36
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 244000052769 pathogen Species 0.000 abstract description 3
- 230000001717 pathogenic effect Effects 0.000 abstract description 3
- 238000007789 sealing Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract 3
- 206010064091 Iatrogenic infection Diseases 0.000 abstract 1
- 239000011358 absorbing material Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 238000001914 filtration Methods 0.000 description 18
- 125000006850 spacer group Chemical group 0.000 description 18
- 239000002250 absorbent Substances 0.000 description 13
- 230000002745 absorbent Effects 0.000 description 13
- 238000000034 method Methods 0.000 description 10
- 208000006454 hepatitis Diseases 0.000 description 9
- 231100000283 hepatitis Toxicity 0.000 description 9
- 229920003023 plastic Polymers 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 230000000274 adsorptive effect Effects 0.000 description 6
- 239000004033 plastic Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000007769 metal material Substances 0.000 description 4
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 206010029803 Nosocomial infection Diseases 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 210000005239 tubule Anatomy 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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Abstract
The invention provides a portable HBVm fast integrated test device, including the suction head (1) and the test pipe (2) connected on the back end of the suction head (1). The front end of the suction head (1) has a thin pipe nozzle (12) for sucking, the suction head inner space (17) is filled with sample filter material (3) and sample water absorbing material (4) from the front to the end respectively, the fixed column (5) is place in the test pipe (2), the test paper fixed column (5) is fixed with HBVm test strip (6), the tail end of the test pipe has sealing cover (7) which seals the waste in the test pipe (2) after test. Through once sample suction and test with the same background with the test strip (6) in the device, five indexes of HBV can be integrated tested at the same time by naked eyes in a few minutes, which makes the test simpler, safer, faster, and avoids crosstalk interference, the waste after test is sealed in the test pipe, which is good for avoiding pathogen spread and iatrogenic infection, and is good for treating of waste in an environmental way.
Description
Technical field
The present invention relates to medicine equipment and sample detection test paper, particularly a kind of once portable fast joint inspection device of while joint inspection five index of hepatitis b (HBsAg, HBsAb, HBeAg, HBeAb and HBcAb).
Background technology
At present, five indexes of hepatitis b (HBsAg, HBsAb, HBeAg, HBeAb and HBcAb) detects the normal enzyme linked immunosorbent assay (ELISA) that adopts.There is inconvenience in this method: sample 1. to be checked needs hydro-extractor to isolate serum earlier, needs incubator, microplate reader in the detection, washes Medical Devices such as plate machine; 2. complicated operating process detects and need the professional and technical personnel just can finish; 3. can only detect a kind of index at every turn; 4. detect the case load that generally need save some and just can carry out, can not accomplish to detect whenever and wherever possible; 5. detection time long, need 40-60 minute at least.
The present invention is directed to the defective that exists in the prior art, disclose a kind of be applicable to whole blood sample, without any need for instrument and equipment and reagent just can detect in good time suitablely and simple to operate, Quality Control convenient, device for fast detecting that just can a joint inspection five index of hepatitis b in a few minutes.Refuse still is closed in the device after the detection of co-detection device of the present invention, is more conducive to avoid that pathogen disseminates, hospital-acquired infection and be more conducive to the environmental protection treatment discarded object.Apparatus structure is simple, and cost of manufacture is cheap, is particularly suitable for suitability for industrialized production.The joint inspection process is quick, safety, accurate.Device also is easy to carry.
Summary of the invention
Technical scheme of the present invention is:
Described portable hepatitis B two double fast joint inspection devices, it according to the disclosed portable example co-detection device of author's number of patent application 200710050163.0 and number of patent application 200720081309.3, be that fixedly HBsAg test strips, HBsAb test strips, HBeAg test strips, HBeAb test strips and HBcAb test strips form respectively in each surperficial shallow grooves 22 of the test paper fixed leg 5 of the disclosed device of above-mentioned application number specifically.Described device comprises suction head 1 and is connected the detector tube 2 of suction head 1 rear end.
Built-in test paper fixed leg 5 in the detector tube 2 is fixed with the test strip 6 that is used to detect hepatitis B two double five indices on this test paper fixed leg 5.The test paper fixed leg 5 that is arranged in detector tube inner chamber 20 constitutes one by head end colyliform spacer ring 15 with the test paper fixed leg shaft 16 that is connected head end colyliform spacer ring 15.Spacer ring 15 diameter of sections and detector tube prosopyle 10 internal diameters and suction head deutostoma 8 internal diameter fits and with the sample filtering material 3 in the suction head inner chamber 17 and 4 block of sample absorbent material in suction head inner chamber 17.The double wedge structure 18 that spacer ring week circumferentially stretched out along the oriented detector tube inwall of distance along certain week of being separated by, this double wedge structure 18 is fixed test paper fixed leg 5 in detector tube inner chamber 20 with recessed toothing 19 tablings of detector tube inwall corresponding position.Also radially have five test strips through holes 21 that pass through all edges of spacer ring and connect detector tube inner chamber 20 and suction head inner chamber 17 between the adjacent double wedge structure 18 on all edges of spacer ring, through hole 21 bores and test strips 6 width, thickness adapt.Be formed with a shallow grooves 22 that is used for fixing test strips separately along the extension of test paper fixed leg shaft 16 surface longitudinals in spacer ring week separately along each the test strips through hole 21 between the adjacent double wedge structure 18, each shallow grooves bottom land is fixed with a test strips 6, in each groove test strips by spacer ring week the edge each corresponding test strips through hole 21 be communicated with sample absorbent material 4 interference in the suction head inner chamber 17 respectively.The inwall of detector tube inner chamber 20 rear ends is fit to locate to be provided with test paper fixed leg 5 tail ends and ends screens 30.
Divide groove to be fixed on its sample suction end 23 of each test strips in the test paper fixed leg 5 surperficial shallow grooves 22, be followed successively by test strips gold mark thing pad district 24 afterwards, test strips detects colour developing district 25, the strong adsorptive pads of test strips tail end district 26 and test strips logo area 27 is installed preceding.
The detection colour developing district 25 sample detection line 28 and the sample nature controlling lines 29 of being provided with separated by a distance of each test strips, wherein detection line 28 is positioned at before the nature controlling line 29.
The test strips 6 that sealing is fixed in described co-detection device test paper fixed leg 5 each shallow grooves 22 of surface is respectively HBsAg test strips, HBsAb test strips, HBeAg test strips, HBeAb test strips and HBcAb test strips.
Wherein, the detection line 28 of HBsAg test strips has been caught the HBsAg monoclonal antibody, and the detection line 28 of HBsAb test strips has been caught HBsAg, and the detection line 28 of HBeAg test strips has been caught the HBeAg monoclonal antibody, the detection line 28 of HBeAb test strips has been caught HBeAg, and the detection line 28 of HBcAb test strips has been caught HBcAg.
The gold mark thing pad district 24 of HBsAg test strips and HBeAg test strips correspondence respectively catches the colloid gold label thing of HBsAg monoclonal antibody (mouse-anti people) and the colloid gold label thing of HBeAg monoclonal antibody (mouse-anti people), and the gold mark thing pad district 24 of HBsAb test strips, HBeAb test strips and HBcAb test strips catches the colloid gold label thing of mouse-anti human IgM antibody or/and mouse-anti human IgG antibody's colloid gold label thing.
The nature controlling line 29 of each test strips is caught the Quality Control thing of one or more the above-mentioned antibody in sheep anti mouse IgM antibody, sheep anti-mouse igg antibody, the anti-mouse IgM of rabbit antibody, the rabbit anti-mouse igg antibody.
Discarded object was enclosed in the detector tube 2 to prevent that detecting the back discarded object spills after the detector tube tail end of described co-detection device had capping 7 to detect.
Detector tube 2 shells and the detector tube tail end capping 7 of described co-detection device are that plastic material is made.
That part of detector tube shell that each test strips of described co-detection device detects colour developing district 25, the strong adsorptive pads of test strips tail end district 26 and test strips installation logo area 27 correspondences is transparent.
Described co-detection device can be cylinder type, flat cylinder-type, rectangular build, prismatic build or other cylinder-type.
The method of manipulating of described portable hepatitis B two double fast joint inspection devices is:
(1) takes out fast joint inspection device;
(2) remove the device headgear lid 31 of described device and the rubber cap bag 14 of suction head front end, expose sample suction port 13, suction nozzle 12 be impregnated in the inspection product;
(3) observing the respective detection line 28 that each built-in test strips detects colour developing district 25 in a few minutes in detector tube transparent plastic district has or not red line to manifest, and with reference to each test strips nature controlling line 29 chromogenic line, whether judgement sample contains HBsAg, HBsAb, HBeAg, HBeAb and HBcAb.Test strips detection line 28 and nature controlling line 29 show red line, and the testing result that this indicated test strips of sign is installed is positive; Nature controlling line 29 shows red line, and detection line 28 redfree lines show that the testing result that this indicated test strips of sign is installed is negative; Nature controlling line 29 redfree lines show that testing result is invalid.
When suction nozzle 12 impregnated in sample to be checked, sample was inhaled in the suction head inner chamber 17 by the suction nozzle capillarity.Sample at first touches sample filtering material 3 and is filtered in suction head inner chamber 17.If sample is a whole blood, filtering material 3 can stop the red blood cell in the blood to pass through, but can allow its serum filter.Blood serum sample after the filtration touches the sample absorbent material 4 in the suction head inner chamber 17 subsequently, and the sample after 4 pairs of filtrations of absorbent material plays auxiliary absorption on the one hand, also has on the other hand to store the function of filtering the back sample.Because each the built-in test strips 6 in the device itself is that interference is communicated with absorbent material 4 in the suction head inner chamber 17, and each test strips tail end is provided with strong adsorptive pads 26, therefore, absorbing, be stored in after the filtration in the absorbent material 4 sample just can be by the test strips siphon principle, is fixed on each test strips 6 rear end in the shallow grooves 22 of test paper fixed leg surface to minute groove smoothly and oozes and move and realize the joint inspection of sample five index of hepatitis b.
When the sample after the filtration contacts test strips, if a kind of among HBsAg, HBsAb, HBeAg, HBeAb and the HBcAb or several are arranged in sample, they just mark the corresponding colloid gold label thing combination in thing pad district 24 with corresponding test strips gold, ooze the corresponding antibody that moves to test strips detection line 28 and contained with detection line 28 or antigen generation idiosyncrasy afterwards and make detection line 28 colour developings, can judge sample to be checked from the colour developing situation and contain which kind of hepatitis B index.Quality Control thing sheep anti mouse or rabbit anti-mouse antibody (one or more in sheep anti mouse IgM antibody, sheep anti-mouse igg antibody, the anti-mouse IgM of rabbit antibody, the rabbit anti-mouse igg antibody) are caught in each test strips nature controlling line 29 position of co-detection device of the present invention, and this Quality Control thing itself can not react with antibody or the antigen in the human blood sample.(that is: the gold of HBsAg test strips mark thing pad district 24 has caught gold mark HBsAg monoclonal antibody and the test strips of co-detection device of the present invention gold mark thing pad district 24 catches gold mark mouse source antibody, the gold mark thing pad district 24 of HBeAg test strips has caught gold mark HBeAg monoclonal antibody, the HBsAb test strips, the gold mark thing pad district 24 of HBeAb test strips and HBcAb test strips has caught gold mark mouse-anti human IgM antibody or/and gold mark mouse-anti human IgG antibody), they together ooze with sample and can react with Quality Control thing sheep anti mouse or the rabbit anti-mouse antibody that nature controlling line 29 is caught when moving to nature controlling line 29, make the colour developing of nature controlling line 29 positions and are identified by naked eyes.When in the sample detection because the quality problems of certain component can't detect test the time, just can not develop the color in nature controlling line 29 positions of test strips, so the Quality Control thing of catching by this nature controlling line can carry out quality control to the sample joint inspection.
The object of the invention:
First purpose of the present invention is to disclose a kind of quick reaction device of joint inspection five index of hepatitis b (HBsAg, HBsAb, HBeAg, HBeAb and HBcAb) simultaneously.
Another object of the present invention is to provide the method for manipulating of described joint inspection reaction unit.
A further object of the present invention is the range of application of described joint inspection reaction unit of explanation and test strips thereof.Specifically, joint inspection reaction unit of the present invention is used for disposable joint inspection sample and whether has HBsAg, HBsAb, HBeAg, HBeAb and HBcAb, and is used in particular for: 1. hospital admission patients'blood hepatitis B two double detections; 2. blood donor's blood screening; 3. various forms of practitioner's health check-ups comprise the preceding health check-up of going abroad, premarital checkup, conscription health check-up, enrollment health check-up and diet, the health check-up of nurseries and kindergartens industry; 4. other need detect HBsAg, HBsAb, HBeAg, HBeAb and HBcAb and detection side simultaneously and overweight each application market that negative findings is judged.
Beneficial effect of the present invention:
(1) once inhales sample and can finish the detection of sample five index of hepatitis b.
(2) each test strip divides groove to be enclosed in the reaction unit, once inhales sample, detects with background, and the five index of hepatitis b of sample is detected become not only simpler, safe, quick, accurate, and the quality control and avoided the detection cross interference of being more convenient for.
(3) testing process is totally-enclosed carries out in an airtight detector tube.During detection, operating personnel only need take off the device headgear lid of described device and the rubber helmet of suction head front end exposes suction nozzle, suction nozzle be impregnated in sample, just can observe directly the every testing result on the test strips in it in a few minutes in described Device Testing pipe clear area, operating personnel do not contact the inspection product repeatedly, and detect the back discarded object and still be enclosed in the detector tube, not only avoided the pathogen in the inspection product to disseminate and hospital-acquired infection, and more helped environmental requirement and offal treatment.
(4) blood sample does not need to isolate serum or blood plasma, just can directly detect with whole blood sample.Prior art is treated the sample product need isolate serum earlier with hydro-extractor, detects with serum.Because the red blood cell in the sample not only can influence sample and ooze smoothly on test strips and move, and the similar observation and the judgement that can influence the result of red color that manifested of erythrocytic red color and detection line and nature controlling line.Be filled with the sample filtering material in the suction head of co-detection device of the present invention, this filtering material can stop in the blood sample red blood cell by and only allow the serum liquid in the sample filter.Therefore, the present invention does not need sample is carried out serum or separating plasma, just can directly detect with whole blood sample.
(5) apparatus structure is simple, and is with low cost, is particularly suitable for suitability for industrialized production.
(6) described device is easy and simple to handle, and is easy to carry, can use in good time suitablely.Its operation does not need special study and training, and professional and technical personnel and ordinary person are all applicable, detects and is not subjected to restrictions such as instrument and equipment and environmental baseline.
(7) reagent and the material that is fixed in the device do not relate to active inactivation, and described co-detection device can be in the room temperature long preservation.
Description of drawings
Fig. 1 is the structure side view of portable hepatitis B two double fast joint inspection devices of the present invention.
Fig. 2 is that portable hepatitis B two double fast joint inspection devices shown in Figure 1 are enclosed the apparatus structure synoptic diagram that device headgear lid back forms at its suction head one end.
In above-mentioned Fig. 1-device shown in Figure 2,1, suction head, 2, detector tube, 3, the sample filtering material, 4, the sample absorbent material, 5, the test paper fixed leg, 6, test strips, 7, the capping of detector tube tail end, 8, the suction head deutostoma, 9, the vertical flange of suction head deutostoma inwall, 10, the detector tube prosopyle, 11, the detector tube prosopyle is indulged recessed inwall, 12, suction nozzle, 13, sample suction port, 14, the rubber helmet, 15, test paper fixed leg head end colyliform spacer ring, 16, test paper fixed leg shaft, 17, the suction head inner chamber, 18, spacer ring week is along the double wedge structure, 19, the recessed toothing of detector tube inwall, 20, the detector tube inner chamber, 21, the test strips through hole, 22, test paper fixed leg post table shallow grooves, 23, the test strips sample suction end, 24, test strips gold mark thing pad district, 25, test strips detects colour developing district, 26, the strong adsorptive pads of test strips tail end district, 27, test strips is installed logo area, 28, test strips detects colour developing district detection line, 29, test strips detects colour developing district nature controlling line, 30, test paper fixed leg tail end ends screens, 31, device headgear lid, 32, hang not
Fig. 3 is that No. 1 blood sample detects synoptic diagram.Testing result shows that No. 1 blood sample does not contain HBsAg, HBsAb, HBeAg, HBeAb and HBcAb.
Fig. 4 is that No. 2 blood samples detect synoptic diagram.Testing result shows that No. 2 blood sample only contains HBsAb.
Fig. 5 is that No. 3 blood samples detect synoptic diagram.Testing result shows that No. 3 blood sample contains HBsAg, HBeAg and HBcAb.
Fig. 6 is that No. 4 blood samples detect synoptic diagram.Testing result shows that No. 4 blood sample contains HBsAg, HBeAb and HBcAb.
Fig. 7 is that No. 5 blood samples detect synoptic diagram.Testing result shows that No. 5 blood sample contains HBsAg and HBeAg.
Among above-mentioned Fig. 3-Fig. 7, T represents detection line (test), and C represents nature controlling line (control)
Embodiment
Fig. 1 is the structure side view of portable hepatitis B two double fast joint inspection devices of the present invention.It according to the disclosed portable example co-detection device of author's number of patent application 200710050163.0 and number of patent application 200720081309.3, be that fixedly HBsAg test strips, HBsAb test strips, HBeAg test strips, HBeAb test strips and HBcAb test strips form respectively in each surperficial shallow grooves 22 of the test paper fixed leg 5 of the disclosed device of above-mentioned application number specifically.Described device comprises suction head 1 and is connected the detector tube 2 of suction head 1 rear end.
Built-in test paper fixed leg 5 in the detector tube 2 is fixed with the test strip 6 that is used to detect hepatitis B two double five indices on this test paper fixed leg 5.The test paper fixed leg 5 that is arranged in detector tube inner chamber 20 constitutes one by head end colyliform spacer ring 15 with the test paper fixed leg shaft 16 that is connected head end colyliform spacer ring 15.Spacer ring 15 diameter of sections and detector tube prosopyle 10 internal diameters and suction head deutostoma 8 internal diameter fits and with the sample filtering material 3 in the suction head inner chamber 17 and 4 block of sample absorbent material in suction head inner chamber 17.The double wedge structure 18 that spacer ring week circumferentially stretched out along the oriented detector tube inwall of distance along certain week of being separated by, this double wedge structure 18 is fixed test paper fixed leg 5 in detector tube inner chamber 20 with recessed toothing 19 tablings of detector tube inwall corresponding position.Also radially have five test strips through holes 21 that pass through all edges of spacer ring and connect detector tube inner chamber 20 and suction head inner chamber 17 between the adjacent double wedge structure 18 on all edges of spacer ring, through hole 21 bores and test strips 6 width, thickness adapt.Be formed with a shallow grooves 22 that is used for fixing test strips separately along the extension of test paper fixed leg shaft 16 surface longitudinals in spacer ring week separately along each the test strips through hole 21 between the adjacent double wedge structure 18, each shallow grooves bottom land is fixed with a test strips 6, in each groove test strips by spacer ring week the edge each corresponding test strips through hole 21 be communicated with sample absorbent material 4 interference in the suction head inner chamber 17 respectively.The inwall of detector tube inner chamber 20 rear ends is fit to locate to be provided with test paper fixed leg 5 tail ends and ends screens 30.
Divide groove to be fixed on its sample suction end 23 of each test strips in the test paper fixed leg 5 surperficial shallow grooves 22, be followed successively by test strips gold mark thing pad district 24 afterwards, test strips detects colour developing district 25, the strong adsorptive pads of test strips tail end district 26 and test strips logo area 27 is installed preceding.
The detection colour developing district 25 sample detection line 28 and the sample nature controlling lines 29 of being provided with separated by a distance of each test strips, wherein detection line 28 is positioned at before the nature controlling line 29.
The test strips 6 of dividing groove to be fixed in described co-detection device test paper fixed leg 5 each shallow grooves 22 of surface is respectively HBsAg test strips, HBsAb test strips, HBeAg test strips, HBeAb test strips and HBcAb test strips.
Wherein, the HBsAg monoclonal antibody has been caught in detection line 28 positions of HBsAg test strips, HBsAg has been caught in detection line 28 positions of HBsAb test strips, the HBeAg monoclonal antibody has been caught in detection line 28 positions of HBeAg test strips, HBeAg has been caught in detection line 28 positions of HBeAb test strips, and HBcAg has been caught in detection line 28 positions of HBcAb test strips.
The gold mark thing pad district 24 of HBsAg test strips and HBeAg test strips correspondence respectively catches the colloid gold label thing of HBsAg monoclonal antibody (mouse-anti people) and the colloid gold label thing of HBeAg monoclonal antibody (mouse-anti people), and the gold mark thing pad district 24 of HBsAb test strips, HBeAb test strips and HBcAb test strips catches the colloid gold label thing of mouse-anti human IgM antibody or/and mouse-anti human IgG antibody's colloid gold label thing.
The Quality Control thing of one or more the above-mentioned antibody in sheep anti mouse IgM antibody, sheep anti-mouse igg antibody, the anti-mouse IgM of rabbit antibody, the rabbit anti-mouse igg antibody is caught in nature controlling line 29 positions of each test strips.
Discarded object was enclosed in the detector tube 2 to prevent that detecting the back discarded object spills after the detector tube tail end of described co-detection device had capping 7 to detect.
Detector tube 2 shells and the detector tube tail end capping 7 of described co-detection device are that plastic material is made.
That part of detector tube shell that each test strips of described co-detection device detects colour developing district 25, the strong adsorptive pads of test strips tail end district 26 and test strips installation logo area 27 correspondences is transparent.During sample detection, but the colour developing of the band by each test strips in this detector tube shell clear area Direct observation auto levelizer result.
Fig. 2 is that portable hepatitis B two double fast joint inspection devices shown in Figure 1 are enclosed device headgear lid 31 and the apparatus structure synoptic diagram of formation at its suction head 1 one ends.It is other 32 to be provided with portable extension on this device headgear lid 31, and this device headgear covers 31 can be made by plastic material, metal material or other material.
Its overall appearance of above-mentioned co-detection device (comprising co-detection device illustrated in figures 1 and 2) can be cylinder type, flat cylinder-type, rectangular build, prismatic build or other cylinder-type.
Embodiment 2 portable hepatitis B two double fast joint inspection devices are manipulated method
The method of manipulating of portable hepatitis B two double fast joint inspection devices of the present invention is:
(1) takes out fast joint inspection device;
(2) remove the device headgear lid 31 of described device and the rubber cap bag 14 of suction head front end, expose sample suction port 13, suction nozzle 12 be impregnated in the inspection product;
(3) observing the respective detection line 28 that each built-in test strips detects colour developing district 25 in a few minutes in detector tube transparent plastic district has or not red line to manifest, and with reference to each test strips nature controlling line 29 chromogenic line, whether judgement sample contains HBsAg, HBsAb, HBeAg, HBeAb and HBcAb.Test strips detection line 28 and nature controlling line 29 all demonstrate red line, and the testing result that this indicated test strips of sign is installed is positive; Nature controlling line 29 shows red line, and detection line 28 redfree lines show that the testing result that this indicated test strips of sign is installed is negative; Nature controlling line 29 redfree lines show that testing result is invalid.
With described portable hepatitis B two double fast joint inspection device joint inspection outpatient blood, blood sample is provided by Chengdu clinical laboratory of healthcare hospital for women ﹠ children, and the method for manipulating is undertaken by embodiment 2.Testing result is as follows:
No. 1 blood sample does not contain HBsAg, HBsAb, HBeAg, HBeAb and HBcAb, the results are shown in Figure 3;
No. 2 blood sample only contains HBsAb, the results are shown in Figure 4;
No. 3 blood sample contains HBsAg, HBeAg and HBcAb, the results are shown in Figure 5;
No. 4 blood sample contains HBsAg, HBeAb and HBcAb, the results are shown in Figure 6;
No. 5 blood sample contains HBsAg and HBeAg, the results are shown in Figure 7.
This instructions embodiment only is for the design feature that co-detection device of the present invention is described, manipulates method and its application in fast joint inspection blood five index of hepatitis b; special needs to be pointed out is; device of the present invention can also have some modifications; for example; the colloid gold label thing of each test strips is also replaceable to be electroselenium label etc.; therefore; all to any other technical scheme that is equal to replacement or equivalent transformation formation of co-detection device employing of the present invention, all drop in the protection domain of claim of the present invention.
Claims (5)
1. portable hepatitis B two double fast joint inspection devices is characterized in that: it according to the disclosed portable example co-detection device of author's number of patent application 200710050163.0 and number of patent application 200720081309.3, be that fixedly HBsAg test strips, HBsAb test strips, HBeAg test strips, HBeAb test strips and HBcAb test strips form respectively in each surperficial shallow grooves (22) of the test paper fixed leg (5) of the disclosed device of above-mentioned application number specifically.
2. portable hepatitis B two double fast joint inspection devices according to claim 1, it is characterized in that: wherein the detection line of HBsAg test strips (28) has been caught the HBsAg monoclonal antibody, the detection line of HBsAb test strips (28) has been caught HBsAg, the detection line of HBeAg test strips (28) has been caught the HBeAg monoclonal antibody, the detection line of HBeAb test strips (28) has been caught HBeAg, and the detection line of HBcAb test strips (28) has been caught HBcAg.
3. portable hepatitis B two double fast joint inspection devices according to claim 1, it is characterized in that: the corresponding respectively colloid gold label thing of HBsAg monoclonal antibody and the colloid gold label thing of HBeAg monoclonal antibody of catching in the gold mark thing pad district (24) of HBsAg test strips and HBeAg test strips, the gold mark thing pad district (24) of HBsAb test strips, HBeAb test strips and HBcAb test strips catches the colloid gold label thing of mouse-anti human IgM antibody or/and mouse-anti human IgG antibody's colloid gold label thing.
4. portable hepatitis B two double fast joint inspection devices according to claim 1 is characterized in that: the nature controlling line of each test strips (29) is caught the Quality Control thing of one or more the above-mentioned antibody in sheep anti mouse IgM antibody, sheep anti-mouse igg antibody, the anti-mouse IgM of rabbit antibody, the rabbit anti-mouse igg antibody.
5. a portable hepatitis B as claimed in claim 1 two double fast joint inspection device and test strips thereof detect the application among HBsAg, HBsAb, HBeAg, HBeAb and the HBcAb at the same time, wherein are particularly related to:
(1) hospital admission patients'blood hepatitis B two double detections;
(2) blood donor's blood screening;
(3) various forms of practitioner's health check-ups comprise the preceding health check-up of going abroad, premarital checkup, conscription health check-up, enrollment health check-up and diet, the health check-up of nurseries and kindergartens industry;
(4) other need detect HBsAg, HBsAb, HBeAg, HBeAb and HBcAb and detection side simultaneously and overweight each application market that negative findings is judged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100452836A CN101246171B (en) | 2008-01-29 | 2008-01-29 | Portable hepatitis B fast joint inspection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100452836A CN101246171B (en) | 2008-01-29 | 2008-01-29 | Portable hepatitis B fast joint inspection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101246171A true CN101246171A (en) | 2008-08-20 |
| CN101246171B CN101246171B (en) | 2012-02-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100452836A Expired - Fee Related CN101246171B (en) | 2008-01-29 | 2008-01-29 | Portable hepatitis B fast joint inspection device |
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| Country | Link |
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| CN (1) | CN101246171B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198687A (en) * | 2014-09-04 | 2014-12-10 | 崔艳香 | Clinical laboratory saliva detector |
| CN104297483A (en) * | 2014-10-15 | 2015-01-21 | 成都领御生物技术有限公司 | Quantum dot immunochromatography test strip for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb and method for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb |
| CN105358977A (en) * | 2013-04-26 | 2016-02-24 | 生物辐射实验室股份有限公司 | Multiplex hepatitis B assay |
| CN105891489A (en) * | 2016-04-08 | 2016-08-24 | 王秀梅 | Test paper for detecting HBcAb (hepatitis B core antibody) IgM (immunoglobulin m) in serum and preparation method of test paper |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2358455Y (en) * | 1999-02-26 | 2000-01-12 | 中国人民解放军第二五三医院 | HBsAg colloidal gold immunochromatography test paper |
| CN2364466Y (en) * | 1999-03-25 | 2000-02-16 | 泉州长立生化有限公司 | Quick HBsAg and HBsAb, HBeAg and HBeAb, and HBcAb testing card |
| JP2002202310A (en) * | 2000-10-27 | 2002-07-19 | Morinaga Milk Ind Co Ltd | Substance detecting reagent and substance detecting method |
| CN201242548Y (en) * | 2008-01-29 | 2009-05-20 | 马义才 | Portable rapid joint inspection apparatus for hepatitis B two and one half |
-
2008
- 2008-01-29 CN CN2008100452836A patent/CN101246171B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105358977A (en) * | 2013-04-26 | 2016-02-24 | 生物辐射实验室股份有限公司 | Multiplex hepatitis B assay |
| US9927439B2 (en) | 2013-04-26 | 2018-03-27 | Bio-Rad Laboratories, Inc. | Multiplex hepatitis B assay |
| CN104198687A (en) * | 2014-09-04 | 2014-12-10 | 崔艳香 | Clinical laboratory saliva detector |
| CN104297483A (en) * | 2014-10-15 | 2015-01-21 | 成都领御生物技术有限公司 | Quantum dot immunochromatography test strip for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb and method for synchronous and quantitative joint inspection of HBsAg, HBeAg and HBcAb |
| CN105891489A (en) * | 2016-04-08 | 2016-08-24 | 王秀梅 | Test paper for detecting HBcAb (hepatitis B core antibody) IgM (immunoglobulin m) in serum and preparation method of test paper |
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| Publication number | Publication date |
|---|---|
| CN101246171B (en) | 2012-02-01 |
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