CN1012440B - Production process of low cost xanthocyte gum - Google Patents
Production process of low cost xanthocyte gumInfo
- Publication number
- CN1012440B CN1012440B CN 87106960 CN87106960A CN1012440B CN 1012440 B CN1012440 B CN 1012440B CN 87106960 CN87106960 CN 87106960 CN 87106960 A CN87106960 A CN 87106960A CN 1012440 B CN1012440 B CN 1012440B
- Authority
- CN
- China
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- gum
- xanthocyte gum
- xanthocyte
- nutrient solution
- acid
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The present invention relates to a method for fermenting and extracting polysaccharide gum by using microorganisms, which belongs to the field of industrial microbiology. In the existing xanthan gum producing method, the xanthan gum is extracted by organic solvents or organic substances by precipitating or direct drying; the existing method has the disadvantages of complicated extracting technology, large energy consumption, multiple devices and high cost. A kind of xanthomonad is screened by the present invention, a culture solution is fermented, acid can be added to the fermented solution to cause gum to be flocculated and deposited, and then, the xanthan gum is extracted. The method of the present invention has the advantages of simple extracting technology, low energy consumption, few devices and low cost.
Description
A kind of method with microbial fermentation polysaccharide glue belongs to the industrial microorganism field.
Xanthocyte gum claims xanthan gum (Xanthan gum) again, is a kind of polysaccharide glue that produces with microbial fermentation.The sixties, the U.S. began to go into operation, and had ten countries and area to produce so far approximately.Domestic Yantai Gourmet Powder Factory beginning in 1987 produced in small quantities, use the extraction using alcohol xanthocyte gum, reclaim through distillation extracting the waste ethanol that produces, energy consumption is big, 1 ton of xanthocyte gum of every production is last because of the ethanol that can not reclaim loss is about 1 ton, and its production cost is about 15000 yuan of/ton dried glue.And other research unit's 1 ton of xanthocyte gum of production will consume 7-10 ton ethanol, so can not go into operation.
Since the sixties, xanthocyte gum was gone into operation in the U.S., many producers adopt bacterial classifications different in the xanthomonas (Xanthomonas) or bacterial strain and diverse ways and produce, the xanthocyte gum series product that are not quite similar have separately appearred, product all has the common characteristic, also some unique distinction and purposes.The production (EP.Pat.66961 1982) of low pyruvic acid content xanthocyte gum is for example arranged; The production (US.Pat.4377637 1983) of low calcium xanthocyte gum; The production of high viscosity xanthocyte gum (J.Pat.52165798 1983); The production of high pyruvic acid xanthocyte gum (US.Pat.4394447 1983); Production of heat-resisting xanthocyte gum (US.Pat.4485020 1984) or the like.
Existing xanthocyte gum extractive technique, mostly be with organic solvent and extract, xanthocyte gum can form even colloidal sol with methyl alcohol, ethanol, Virahol, acetone and other organic solvent, but organic solvent concentration in mixed solution surpasses at 50% o'clock, and xanthocyte gum can form fibrous precipitation and separate out.As U.S. Pat-4316012, its extracting method is exactly to add a kind of organic solvent deposit glue in fermented liquid, again through centrifugal, separate, filter, oven dry makes the xanthocyte gum product, in addition, xanthocyte gum solution can with calcium salt such as CaCl
2, Ca(OH)
2Act on, in organic solvent, form the gel precipitation of xanthocyte gum-calcium hydroxide mixture,, use organic solvent and divalent ion sedimentation xanthocyte gum exactly as European patent EP-0068706.The quaternary amine of employing sedimentation xanthocyte gum is also arranged, and promptly xanthocyte gum separates xanthocyte gum with quaternary amine binding substances generation flocculation sediment.Adopt spraying drying, flash distillation, decompression abroad in addition, concentrate, method such as ultrafiltration extracts xanthocyte gum.
Extract xanthocyte gum at present both at home and abroad and all adopt convection drying and organic solvent deposit to extract two class methods, the former is methods such as spraying drying, flash distillation, concentrating under reduced pressure, and energy consumption is too big, and is unworkable in China; The latter be unable to do without expensive organic solvent, and the recovery of solvent slop is very power consumption also.The present invention has screened a kind of Xanthomonas campestris Xanthomonas sp.8420(CGMCC0131), its fermented liquid can be used sour flocculating settling, reduces material consumption, energy consumption, the purpose of reduce cost, equipment is few, simple and easy to do thereby reach.Domestic and international existing xanthocyte gum production technology does not all have the acid system of use flocculating settling.
The present invention utilizes a kind of Xanthomonas campestris fermentation, and the production method of preparation Xanthomonas campestris polysaccharide glue is characterized in that nutrient solution ferments through Xanthomonas campestris, fermentating liquid acidification, and precipitation is extracted xanthocyte gum.The acid that in fermented liquid, adds, available mineral acid or organic acid, the pH value is transferred to below 3, and the xanthocyte gum flocculation sediment is extracted.Fermented liquid is except adding acid, also can be aided with and add other salt, help the condensate precipitation, extract xanthocyte gum as calcium salt, Repone K etc., it is Xanthomonas sp.8420(CGMCC0131 that xanthocyte gum is produced used bacterial classification), can use full-inorganic nitrogenous source nutrient solution and produce xanthocyte gum.The production method of xanthocyte gum is as follows:
One, bacterial classification
Xanthomonas campestris Xanthomonas sp.8420(CGMCC0131) in screening gained in 1984, this bacterial classification was deposited in Beijing China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number 0131.This bacterium is characterized as shaft-like, Gram-negative, and end is given birth to a flagellum, produces yellow water-insoluble pigment on gravy peptone substratum, bacterium colony garden shape, lawn is not expanded, yellow.Oxidizing glucose, the pigment that is produced have 418,437 and 463 millimicrons of absorption peaks in sherwood oil.
Xanthomonas campestris Xanthomonas sp.8420(CGMCC0131 of the present invention) nutrient solution that can use the full-inorganic nitrogenous source is produced xanthocyte gum.
Two, substratum
1, slant strains substratum: (prescription derives from US.Pat.4394447.1983.7.9)
Glucose 1.2% (NH
4)
2HPO
40.15%
Yeast extract paste 0.15% K
2HPO
40.25%
Peptone 0.25% MgSO
47H
2O 0.01%
Tap water 96.29%
Agar 1.7% pH7
Sterilized 30 minutes for 15 pounds.
2, first class inoculum shakes the bottle substratum:
The same, no agar.
3, second class inoculum (or seeding tank) nutrient solution:
NaNO
30.2% MgSO
4·7H
2O 0.05%
K
2HPO
40.25% FeSO
40.001%
Sugar (sucrose or glucose) 1% tap water 98.499% pH 7.5
Sterilized 30 minutes for 15 pounds,, add then if use glucose, glucose with 8 pounds of 20 minutes otherwise sterilized.
4, produce nutrient solution:
The nutrient solution prescription is the same, but sugared available sucrose, glucose or starch saccharificating liquid.
Feed supplement twice between yeast phase, and each feed supplement comprises the sugar that accounts for whole fermented liquids 1.5% and 0.05% vegetables oil, and in the whole fermentation period, dropping into the total reducing sugar amount in the nutrient solution is 4%.Sucrose sterilization be 15 pounds 30 minutes, the glucose sterilization be 8 pounds 20 minutes.
The nutrient solution major nitrogen source is from the NaNO of inorganic states
3Or KNO
3, NH
4NO
3, urea or mend again and add yeast extract paste, peptone, fish meal etc., or mend and add other various micro-persons.
Three, zymotechnique
1, process:
Slant strains (28 ℃ ± 2 ℃)/(2-3 days) first class inoculum shaking culture (28 ℃ ± 2 ℃)/(24h.) seeding tank (28 ℃ ± 2 ℃)/(24h.) is produced jar
The 24-30h. of feed supplement for the first time feed supplement for the second time 36-42h.
↓ ↓
(28-30 ℃)/(60h.) puts jar
The test tube slant bacterial classification was cultivated 2-3 days in 28 ℃.First class inoculum uses 500ml triangular flask, every bottled 150ml substratum, test tube slant bacterial classification inoculation of every bottle of usefulness, 28 ℃ of shaking tables are cultivated 24 hours (bacteria containing amount reaches more than 800,000,000/ml), the seeding tank inoculum size is 2-4%, air-blowing quantity be the 0.5-1 volume/minute, tank pressure 1kg/cm
2, temperature 28-30 ℃.Producing a jar inoculum size is 7-10%, air-blowing quantity 0.8-1 volume/minute, tank pressure 1kg/cm
228 ± 2 ℃ of jar temperature, stirring velocity was 100rpm in stirring velocity 100-200rpm(1-24 hour, increased progressively backward, after 36 hours, stirring velocity 200rpm) feed supplement therebetween twice, feed supplement when residual sugar reduces to 0.2% in the culture material, generally 24-30 hour and feed supplement in 36-42 hour each once.
2, equipment requirements
(1) shaking table: reciprocation type or rotary all available, reciprocation type shaking table speed is more than 100 times/minute, rotary shaking table speed is more than 170 rev/mins.
(2) fermentor tank: this is full-bodied fermented liquid, and jar is high bigger than suitable with the jar diameter, be desirably 5: 1 most, if the original used equipment of employing is advisable to be not less than 2.5: 1.Air-blowing quantity be not less than 1 volume/minute, more than the stirring velocity 200rpm.High according to jar, its stirring arm should be three to six groups, and the wing should have the hole, whirlpool, makes it to be suitable for the stirring of high viscosity liquid.Fermentor tank also should have corresponding feed supplement equipment simultaneously.
Four, refining technique
The 60 hours sugared content that ferments is reduced to below 0.2%, can put jar.Put jar secondary fermentation liquid available metal or ceramic cylinder groove and contain, slowly add 1: 20 hydrochloric acid, and stir, drop at 2.6 o'clock, glue and water sepn, available mechanical dehydration to fermented liquid pH with the speed of 6-10rpm.(fermented liquid that this method produced produces the glue separation phenomenon when pH2.6, but this to be reversible change, the dried glue recovery that can absorb water in the application, viscosity is constant substantially).Mechanical dehydration can adopt whizzer or filter press, can remove glue that 6/7 to 7/8 moisture content will take off water again in 55-65 ℃ of oven dry, pulverizes, and crosses the 0.25mm sieve, and water content must not surpass 7%, use plastic bag packaging seal.Fermented 60 hours, every 100ml fermented liquid can produce dried glue 2.87g, and extraction yield is 89.9%, so every 100ml fermented liquid can be done glue 2.58g.The carbon source transformation efficiency is 64.5%.
Five, physico-chemical property
The physico-chemical property of xanthocyte gum product of the present invention:
1, soltion viscosity: 0.5% solution viscosity is 3200 centipoises.
2, pyruvic acid content: 3.1%
3, salt is to the influence of viscosity:
5% to 10%Na
2CO
3The solution medium viscosity is constant.25 ℃, reduce by 16% at 10%NaCl or in KCl solution medium viscosity.
4, heating is to the influence of viscosity:
Be heated to 95 ℃ from 25 ℃ viscosifying action is arranged.Being heated to 95 ℃ of viscosity in 10%KCl changes little.
5, pH is to the influence of viscosity:
Viscosity changes very little in pH3.5 to 11 scope.There is slight tackify to use more than the pH11.Produce throwing out when pH2.6 is following, utilize this characteristic can add diluted acid and extract.
6, thixotropy:
Good thixotropy is arranged, and No. 3 awl rotating speeds of 1% aqueous solution are when per minute increases to 12,30,60 times 6 times, and its viscosity is then reduced to 6800,3320,1990 centipoises from 11,800 centipoises
The xanthocyte gum that product of the present invention and U.S. Kelco company produce is made comparisons, and identical feature is as follows with different features: (1) both viscosity are approaching, use rotational viscosimeter, and 25 ℃, No. 4 rotor 30rpm measures, and its 0.5% viscosity in aqueous solution is 3200 centipoises.
(2) the stable basically identical in salts solution.
(3) product of the present invention is poor slightly to the pyritous resistance, and when 10%KCl existed, it is constant that solution is heated to 95 ℃ of viscosity, freezing in the time of 100 ℃, and 120 ℃ of U.S.'s product abilities.
(4) pyruvic acid content belongs to american commerce product acceptability limit.
(5) U.S.'s product is in the pH1-11 scope, and viscosity only has slight variation, and product of the present invention, in the pH3-11 scope, viscosity only has slight variation, produces flocculation reaction during pH2.6.
(6) both are close for thixotropic property.
Six, measuring method
1, viscosimetric analysis
With the Shanghai balance equipment NDJ-1 of factory rotational viscosimeter, 25 ℃, the solution preparation was measured after 24 hours.
2, gum yield is measured
Claim the 10g fermented liquid to be diluted to 50ml, stir, 20, centrifugal 30 minutes of 000rpm, supernatant liquor precipitates with twice, 60 ℃ of oven dry of absolute ethanol washing with 120ml dehydrated alcohol precipitation xanthocyte gum, weighs with 0.01% balance.
3, residual sugar is measured
100g fermented liquid 15ml1: 20 hydrochloric acid (HCl) precipitation, get filtrate and be neutralized to pH7 with NaOH, measure with Fehlings reagent again.
4, strain identification
The division bacteria basis, the big spade-shaped farm tool used in ancient China of king is compiled Science Press 1977
5, moisture determination
Claim the 1g sample in 60 ℃ of oven dry with 0.01% balance, put in the moisture eliminator, weigh, calculate the moisture content degree.
6, pyruvic acid assay: 0.05% the xanthocyte gum aqueous solution, with pyruvic acid 2, the colorimetric estimation of 4-dinitrophenylhydrazone method.
7, bacterial count detects
(1) colony counting method: water plate with substratum 1, locate to cultivate 10 days countings for 28 ± 2 ℃.
(2) turbidimetry: cultivated bacterium liquid 36 hours with substratum 3, adding sterilized water again is diluted to and contains original bacteria liquid 100%, 90%, 80%, 70%, 60%, 50%, 40% various extent of dilution are surveyed number with colony counting method on the one hand, and with same series of samples than turbid, draw the expression bacterium number typical curve relevant with turbidity.When mensuration inoculation liquid bacterium is counted,, just can find the bacterium number from curve through surveying turbidity.
8, ash mensuration takes by weighing the 10g sample with 0.01% balance and places 550 ℃ of muffle furnaces burnings 1 hour, weighs, and calculates percent ash.
Seven, the purposes of xanthocyte gum
Xanthocyte gum is had many uses, and is applicable to that petroleum industry makes mud additive; Be used for foodstuffs industry and make emulsifying agent, thickening material, suspension agent, foaming aids etc.; Also be used for industry such as textile printing and dyeing, coating, ceramic glazing, colloidal explosive, fire-retardant material, medicine and agricultural chemicals.The application of xanthocyte gum can cover more than 20 industry, and tens kinds of products greatly improve the technology of these industries, product quality.80 tons of the annual import xanthocyte gums of China are because high price (13000$/ton) only uses for the onshore oil field drilling well of Sino-foreign joint venture.The demand of the domestic xanthocyte gum in the left and right sides in 2000 is at least about the 5000-10000 ton according to estimates.Wherein the biggest market is an oil sector, is mud conditioner if annual 1/4 oil well adopts xanthocyte gum, just needs the 3500-5000 ton.Xanthocyte gum has good rheological, and thickening, anti-salt, filtrate-loss control can be good, the oil offtake that is significantly improved and prevent the effect of blowout.
By the inventive method, the xanthocyte gum sample of Laboratory Production has been sent to the North China Oilfield test for three times, think have good water solubility, dosage is little, reduce swivel viscosity obviously, the shear thinning performance is strong, fall premium properties such as dehydration.Think that this is a kind of new treatment agent that is suitable for polymer mud of current optimum.
Eight, precaution
1, the fermentor tank aspect ratio is suitable big, if may be preferably 5: 1.
2, according to the aspect ratio of fermentor tank, stirring rake should be the 3-6 group in jar.
3, stir the paddle number: the upper strata can be 3 wings, and lower floor should be 6 wings, should be porose in the wing, stir to be fit to high viscosity liquid.
When 4, producing the jar fermentation, stirring velocity can be 100rpm in preceding 24 hours, and along with the generation of glue, stirring velocity should increase to more than the 200rpm after increasing by 36 hours.
5, produce a jar inoculum size and should be 7-10%, too small easy pollution or glue output is descended.
6, air-blowing quantity be the 0.8-1 volume/minute, maybe can reach 1.5 volumes/minute.
When 7, flocculating, concentration of hydrochloric acid is with 1: 20(HCl: H
2O) be advisable, concentration is excessive easily to make the glue sex change, and concentration is too small, is difficult for flocculation and blowdown flow rate and increases.
8, after the flocculation, decorating film and liquid are easy to separately, and the most simple method is use mechanical expression, and the equipment that has according to factory is as if having industrial centrifugal machine, Plate Filtration also can use.
9, the drying room temperature should be between 55-65 ℃.
10, should cross the 0.25mm sieve after drying products is pulverized, and sealed damp-proof, moisture content should be below 7%.
Existing xanthocyte gum production method all is to adopt organic solvent (or organic compound) to extract or convection drying.The present invention has screened special bacterial strain, and the polysaccharide glue that this bacterium produced can produce throwing out in the aqueous solution below pH2.6, so just only need can make the glue flocculating settling with acid as dilute hydrochloric acid, this has made significant improvement in the xanthocyte gum extraction process.Compare with prior art, the present invention has following advantage and positive effect.
1, cost is low: the xanthocyte gum cost that domestic prior art is produced is 15000 yuan/ton, and the xanthocyte gum cost that the present invention produces is 6000-7000 unit/ton.
2, energy consumption is low: one ton of xanthocyte gum of the every extraction of prior art will use alcohol more than 40 tons, and spent organic solvent (alcohol) is reclaimed, and consume a large amount of energy, and the present invention needn't reclaim spent acid, and energy consumption significantly reduces.
3, material consumption economizes: domestic prior art is produced xanthocyte gum, and because of organic solvent can not all reclaim, product per ton consumes organic solvent (as alcohol) more than 1 ton, and the xanthocyte gum product per ton that the present invention produces only need consume 0.25 ton of hydrochloric acid.
4, equipment is few.Prior art also will increase between the spent organic solvent recovery vehicle except that the needs corresponding apparatus.It is low that present device requires, and has spice cylinder, squeezing machine or industrial centrifugal machine get final product, generally Gourmet Powder Factory, the biochemical-pharmaceutical factory production of also all having ready conditions.
The embodiment of the invention is as follows:
Embodiment 1:4.5 rises shaker fermentation
1, slant strains is cultivated: the same substratum 1.Bacterial classification test tube specification is 12 * 150mm, contains substratum 5ml, cultivates 2-3 days in the 28-30 ℃ of thermostat container in inoculation back.
2, second class inoculum is cultivated: the nutrient solution prescription is: NaNO
30.2% MgSO
47H
2O 0.05% K
2HPO
40.25% FeSO
40.001% sugar (sucrose or glucose), 1% tap water, 98.499% pH7.5
Sterilized 30 minutes for 15 pounds, if use glucose, glucose is with 8 pounds of 20 minutes otherwise sterilized.Use the 500ml triangular flask, every bottled nutrient solution 150ml, totally 3 bottles, with the inoculation of above-mentioned cultivation slant strains, 1 bottle of 1 bacterial classification inoculation is 170rpm at rotating speed, cultivates 24 hours for 28-30 ℃ on the rotary shaking table, the bacterium number reaches more than 800,000,000/ml can make seed.
3, shake flask fermentation produces glue: the nutrient solution prescription is the same.With the every bottled nutrient solution 150ml of 500ml triangular flask, totally 30 bottles, with above-mentioned second class inoculum inoculation, every bottle of inoculum size is 15ml, is on the shaking table of 170rpm at rotating speed, cultivated 60 hours for 28-30 ℃, between incubation period, carried out the feed supplement first time in 24-30 hour, every bottle is added 45% liquid glucose 5ml, carried out the feed supplement second time in 36-42 hour, every bottle is added 45% liquid glucose 5ml.The feed supplement time should be according to the mensuration of fermented liquid residual sugar amount, and the residual sugar amount is promptly answered feed supplement below 0.2%.Dropping into the total reducing sugar amount in the nutrient solution is 4%, ferment about 60 hours, and the residual sugar amount reduces to 0.2% when following once again, can stop fermenting.
4, extract xanthocyte gum:
Pouring fermented liquid into plastics, glass or enamel holds in the ware, the dilute hydrochloric acid that slowly injected 1: 20 slowly is stirred to pH with thick glass rod for 0.5 liter and reduces to below 2.6, glue in the fermented liquid flocculates at this moment, can finish flocculating settling about 10 minutes, static 30-60 minute, filter with 1mm nylon mesh or nylon cloth, use the available gravity extrusion of sieve, use nylon cloth then can with the hands push, this method can be removed the moisture content of 6/7-7/8 in the fermented liquid.
5, producing of finished product:
The above-mentioned micelle of extracting is torn diffusing, put 55-65 ℃ of baking oven and do oven dry, pulverize, cross the 0.25mm sieve, store in glass bottle or the plastics bag with micromill.
Embodiment 2:0.1 ton ferment tank
1, slant strains is cultivated: bacterial classification test tube specification is 15 * 200mm, contains substratum 8ml, and other is with example 1.
2, second class inoculum is cultivated: the nutrient solution prescription is: NaNO
30.2% MgSO
47H
2O 0.05% K
2HPO
40.25% FeSO
40.001% sugar (sucrose or glucose), 1% tap water, 98.499% pH 7.5
Sterilized 30 minutes for 15 pounds, if use glucose, glucose is with 8 pounds of 20 minutes otherwise sterilized.30 of 1000ml triangular flasks, every bottled nutrient solution 250ml, with the inoculation of above-mentioned cultivation slant strains, 1 bottle of 1 bacterial classification inoculation on speed is 100 reciprocation type shaking tables, was cultivated 24 hours for 28-30 ℃, and the bacterium number reaches and promptly can be used as seed more than 800,000,000/ml.
3, fermentor tank is produced glue: the nutrient solution prescription is the same.Through aseptic technique above-mentioned second class inoculum nutrient solution is merged, all insert fermentor tank, fermentor tank feeds intake 0.08 ton, warm 28-30 ℃ in jar, twice feed supplement between incubation period is for the first time at 24-30 hour, for the second time at 36-42 hour, all decide according to the mensuration of fermented liquid residual sugar amount during the feed supplement, the residual sugar amount is reduced to and is promptly answered feed supplement below 0.2%.The each sterilization sugar soln of 2700ml45% and mixed solution of 0.04 kilogram of vegetables oil of adding, dropping into the total reducing sugar amount in the nutrient solution is 4%.Fermented about 60 hours, the residual sugar amount reduces to 0.2% when following once again, can stop fermentation.
4, extract xanthocyte gum: fermented liquid is poured in plastics, enamel or the stainless steel tank, be preferably the spice bucket of being furnished with stirring arm, slowly inject 1: 20 10 kilograms in dilute hydrochloric acid, the speed of changeing with per minute 6-10 slowly stirs, fully after the flocculation, static 60 minutes, with the speed dehydration of 1000 rev/mins of industrial centrifugal machines, or the squeezing machine dehydration.Can remove the moisture content of 6/7-7/8 in the fermented liquid.
5, finished product is produced: with the glue of above-mentioned dehydration, put in the 55-65 ℃ of drying room and dry, pulverize with pulverizer, cross the 0.25mm sieve, packaging moistureproof.
Embodiment 3:20 ton ferment tank
1, slant strains is cultivated: with example 2.
2, eggplant bottle spawn culture: substratum 1(culture medium prescription is the same).Every bottled substratum 50ml, totally 50 bottles, the inoculation back is cultivated 28-30 ℃, 2-3 days.
3, seed tank culture: the nutrient solution prescription is: NaNO
30.2% MgSO
47H
2O 0.05% K
2HPO
40.25% FeSO
40.001% sugar (sucrose or glucose), 1% tap water, 98.499% pH 7.5
Sterilized 30 minutes for 15 pounds, if use glucose, glucose is with 8 pounds of 20 minutes otherwise sterilized.Feed intake 1.6 tons, lawn in the eggplant bottle is washed, once insert seeding tank with the nutrient solution 5000ml that sterilizes, warm 28-30 ℃ in jar, air-blowing quantity 0.5 volume/minute, stirring velocity 100-150rpm cultivated 24 hours.The bacterium number reaches and promptly can be used as seed more than 800,000,000/ml.
4, bulk fermentation: the nutrient solution prescription is the same.20 tons of fermentor tanks feed intake 14.4 tons, and 1.6 tons of seed liquor of seeding tank are once inserted, and each residual sugar amount is reduced to feed supplement in 0.2% o'clock.Feed supplement comprises sugar soln and 9 kilograms of vegetables oil of 540 kilogram 45%, and totally 2 times, between 24-30 hour, between 36-42 hour, the total reducing sugar amount that drops in the nutrient solution is 4% for the second time for the first time.Jar warm 28-30 ℃, during 1-24 hour air-blowing quantity be 0.8 volume/minute, stirring velocity 100rpm increases progressively backward, after 36 hours, air-blowing quantity reach 1 volume/minute, stirring velocity 200rpm, tank pressure 1kg/cm
2, jar high and jar diameter are than being desirably most 5: 1, and stirring arm is 6 groups, and starching on the wing has the hole, whirlpool.Ferment after 60 hours, residual sugar reduces to 0.2% when following, and fermentation is finished.
5, finished product is produced: with example 2.
Claims (4)
1, a kind of method of extracting xanthocyte gum from the Xanthomonas campestris fermented liquid is characterized in that fermented liquid adds acid and regulates pH value below PH3, makes the xanthocyte gum flocculation sediment, extracts.
2, production method according to claim 1, the Xanthomonas campestris that it is characterized in that indication are Xantho-monas sp.8420(CGMCC0131), can use full-inorganic nitrogenous source nutrient solution and produce xanthocyte gum.
3, production method according to claim 1 is characterized in that sour available mineral acid or organic acid.
4, production method according to claim 1 is characterized in that can also being aided with outside the fermentating liquid acidification and adds other salt, helps the condensate precipitation to extract xanthocyte gum as calcium salt, Repone K etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87106960 CN1012440B (en) | 1987-10-21 | 1987-10-21 | Production process of low cost xanthocyte gum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 87106960 CN1012440B (en) | 1987-10-21 | 1987-10-21 | Production process of low cost xanthocyte gum |
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| Publication Number | Publication Date |
|---|---|
| CN87106960A CN87106960A (en) | 1988-04-27 |
| CN1012440B true CN1012440B (en) | 1991-04-24 |
Family
ID=4815915
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|---|---|---|---|
| CN 87106960 Expired CN1012440B (en) | 1987-10-21 | 1987-10-21 | Production process of low cost xanthocyte gum |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101185485B (en) * | 2007-12-24 | 2010-09-29 | 山东阜丰生物科技开发有限公司 | Method for extracting xanthan gum used for producing sauce products |
| CN101585886B (en) * | 2009-06-03 | 2012-10-03 | 浙江帝斯曼中肯生物科技有限公司 | Post-extracting method of high acyl gellan gum |
| CN102220394A (en) * | 2010-04-16 | 2011-10-19 | 淄博中轩生化有限公司 | Method for producing transparent xanthan gum |
| CN106167744A (en) * | 2016-08-16 | 2016-11-30 | 梅庆波 | A kind of preparation method of plant antibacterial acaricide liquid detergent |
| CN109762857A (en) * | 2017-11-09 | 2019-05-17 | 卢松 | A kind of technique preparing xanthan gum |
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1987
- 1987-10-21 CN CN 87106960 patent/CN1012440B/en not_active Expired
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