CN101229506A - Heavy metal biological sorbent, preparing method and applications thereof - Google Patents
Heavy metal biological sorbent, preparing method and applications thereof Download PDFInfo
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- CN101229506A CN101229506A CNA2007100310987A CN200710031098A CN101229506A CN 101229506 A CN101229506 A CN 101229506A CN A2007100310987 A CNA2007100310987 A CN A2007100310987A CN 200710031098 A CN200710031098 A CN 200710031098A CN 101229506 A CN101229506 A CN 101229506A
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Abstract
The invention discloses a biosorbent of heavy metal, a preparation method thereof and application. Nutritive materials are added into residual activated sludge and C/N and pH are respectively adjusted to 5:1-8:1 and 5-7 so as to obtain microbial media in which the yeast is inoculated in shaking culture at the temperature of 30-37 DEG C and the speed of 100-200rpm for 25-35h and then is centrifuged for 5-10min at 3000-4000rpm; the collected bacterial sludge is the biosorbent of heavy metal. The sorbent is added into the heavy metal waste water with quality concentration ratio between the sorbent and the heavy metal waste water of 5-10g/L:10-20mg/L, table shaken with rotational speed at 50-200rpm for 2-8h and the treatment of heavy metal waste water can be realized by centrifugalization. The invention can reduce production cost of the sorbent, save operation cost of sewage treatment plants and improve the treatment efficiency.
Description
Technical field
The present invention relates to the development field of adsorbent, particularly a kind of Biosorbent for heavy metals and its production and application.
Background technology
The environmental hazard that a large amount of heavy metal-containing waste water brought of industries such as plating, mining, smelting, chemical industry, weaving, printing and dyeing, medicine, paint, alloy, papermaking, pottery discharging has caused the extensive concern of government and various circles of society.The processing method of tradition heavy metal-containing waste water mainly contains the precipitation method, chemistry redox method, evaporation, ion-exchange, electrochemical treatments method, membrane technology partition method etc., the shortcoming that these methods are the most outstanding be to handle low concentration (＜100mg/L) during heavy metal wastewater thereby, complex operation, operating cost height, easily form secondary pollution etc.In recent years, the serious enterprise of some heavy metal pollutions is progressively banned or is rectified and improved, and the discharging of heavy metal is limited to lower concentration range, to reduce the pressure to water body environment.Advantages such as for the processing of these low concentration wastewaters, it is good that biological adsorption technology has selectivity, and antijamming capability is strong, and pH value and temperature accommodation are wide, and can reclaim some precious metals effectively.The complex system that activated sludge is made up of microbial organisms, organic polymer, colloid, mineral grain and metal ion etc. wherein contains nutriments such as abundant nitrogen, phosphorus, has been used to make fertilizer.As seen, these nutritional labelings in the activated sludge can effectively be utilized by biology.While polymer and microbial cell outside the activated sludge surface aggregation a large amount of born of the same parents; outer polymer of born of the same parents and cell membrane mainly are made up of protein, humic acid, uronic acid, polysaccharide and a spot of lipid and nucleic acid; these contain groups such as a large amount of carboxyls, hydroxyl, phosphoryl, sulfuric acid ester, amino and acylamino-in forming; these groups can form complex compound and chelate on the activated sludge surface with heavy metal ion, and heavy metal ion is adsorbed removal in the solution thereby make.But expensive production cost has limited the commercial application of biological adsorption agent.Exploitation is cheap, biological adsorption agent has become the key areas of heavy metal containing wastewater treatment efficiently.
At the characteristics of activated sludge, activated sludge is used for the preparation of biological adsorption agent, can provide nutrition for the growth of efficient absorption bacterial strain on the one hand, on the other hand can the associated treatment heavy metal ion.Both can alleviate the expense that enterprise pollution is administered, mud is reasonably disposed, have tangible economic benefit, social benefit.Be particularly useful for discharging the enterprise of the industrial wastewater that contains heavy metal ion, as machining, mining industry, the smelting of iron and steel and non-ferrous metal and part chemical enterprise etc., the treatment of wastes with processes of wastes against one another, cost is low.The excess sludge of sewage treatment plant mainly is made of the residual body of a large amount of microorganisms and organic substance, inorganic matter, contains a large amount of organic matters and nutriments such as the nitrogen that enriches, phosphorus in the mud, increases the nutrient medium that suitable carbon source just can be used as microorganism.Simultaneously, activated sludge itself contains abundant organism heavy metal is had certain suction-operated.Therefore, utilize the activated sludge of sewage treatment plant to cultivate the manufacturing cost that microbial adsorbent both can reduce biological adsorption agent, can improve removal efficiency of heavy metals again.
Summary of the invention
To the objective of the invention is the deficiency that exists in the above-mentioned technology in order solving, a kind of Biosorbent for heavy metals and its production and application to be provided.
Purpose of the present invention is achieved through the following technical solutions:
The preparation method of a kind of Biosorbent for heavy metals of the present invention may further comprise the steps:
(1) get excess sludge, add nutriment in mud, regulating C/N is 5: 1～8: 1, pH5～7, with this as microbiological culture media.
(2) inoculation yeast is in 30～37 ℃ in the microbiological culture media in (1), and after 100～200rpm velocity fluctuation was cultivated 25～35h, the centrifugal 5～10min of 3000～4000rpm collected bacterium mud, and this bacterium mud is Biosorbent for heavy metals.
Described nutriment comprises that glucose, fructose, sucrose, lactose, maltose, yeast soak powder, peptone, MgSO
4In a kind of or more than one.
Described yeast is one or more in Candida lipolytica (Candida lipolytica), shadow yeast (Sporobolomycetaceae), candida utili (Candida utilis), the saccharomyces cerevisiae (Saccharomyces cerevisiae).
With method for preparing Biosorbent for heavy metals of the present invention.
Biosorbent for heavy metals with method preparation of the present invention can be handled heavy metal wastewater thereby: adsorbent is added in the heavy metal wastewater thereby, make adsorbent: the quality volumetric concentration ratio of heavy metal wastewater thereby is 5～10g/L: 10～20mg/L, shaking table with 50-200rpm velocity fluctuation 2～8h after with the centrifugal 5～10min of 2500～5000rpm speed.
The present invention compares with prior art, has following advantage and effect:
(1) prepares biological adsorption agent with sludge of sewage treatment plant as the cultivation material and can reduce the adsorbent production cost greatly, be beneficial to industrial applications.
(2) realize the recycling of excess sludge, turned waste into wealth, saved the operating cost of sewage treatment plant.
(3) heavy metal ion in the waste water is removed in activated sludge-heavy metal absorption bacterium synergy, can improve treatment effeciency greatly.
The specific embodiment
The present invention is further illustrated below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
Embodiment 1: hunt moral sewage treatment plant from the Guangzhou and gather returned sludge, get above-mentioned mud 150g, add sucrose 20g, making C/N is 8: 1, is settled to 1000ml then, regulates pH6, as microbiological culture media.Inoculation candida utili shaken cultivation (200rpm) in shaking table in this fluid nutrient medium, 32 ℃ of cultivation temperature, incubation time 33h, the centrifugal 5min of 3000rpm obtains adsorbent.Add this adsorbent 10g/L, handle the 15mg/L chrome liquor, shaking table can reach 95% with the centrifugal 5min clearance of 2500rpm speed after with 50rpm velocity fluctuation 2h.
Embodiment 2: hunt moral sewage treatment plant from the Guangzhou and gather returned sludge, get above-mentioned mud 100g, add and contain glucose 10g, making C/N is 5: 1, is settled to 1000ml then, regulates pH7, as microbiological culture media.Inoculation Candida lipolytica shaken cultivation (100rpm) in shaking table in this fluid nutrient medium, 30 ℃ of cultivation temperature, incubation time 25h, the centrifugal 8min of 3500rpm obtains adsorbent.Add this adsorbent 6g/L, handle the 10mg/L chrome liquor, shaking table can reach 98% with the centrifugal 10min clearance of 5000rpm speed after with 200rpm velocity fluctuation 8h.
Embodiment 3: hunt moral sewage treatment plant from the Guangzhou and gather returned sludge, get above-mentioned mud 200g, add fructose 15g, making C/N is 6: 1, is settled to 1000ml then, regulates pH5, with this as microbiological culture media.In aforesaid liquid inoculation of medium shadow yeast shaken cultivation (150rpm) in shaking table, 37 ℃ of cultivation temperature, incubation time 35h, the centrifugal 10min of 4000rpm, collected bacterium mud is Biosorbent for heavy metals.Add this adsorbent 8g/L, handle 20mg/L copper liquid, shaking table with 150rpm velocity fluctuation 6h after with the centrifugal 6min of 3000rpm speed, clearance can reach 90%.
Embodiment 4: hunt moral sewage treatment plant from the Guangzhou and gather returned sludge, get above-mentioned mud 150g, add lactose 16g, making C/N is 7: 1, is settled to 1000ml then, regulates pH6, as microbiological culture media.In this fluid nutrient medium, inoculate saccharomyces cerevisiae, Candida lipolytica shaken cultivation (200rpm) in shaking table, 32 ℃ of cultivation temperature, incubation time 33h, the centrifugal 5min of 3000rpm obtains adsorbent.Add this adsorbent 5g/L, handle 15mg/L nickel liquid, shaking table with 100rpm velocity fluctuation 7h after with the centrifugal 8min of 4000rpm speed, clearance can reach 96%.
Embodiment 5: hunt moral sewage treatment plant from the Guangzhou and gather returned sludge, get above-mentioned mud 150g, add maltose 20g, MgSO
40.05g making C/N is 8: 1, is settled to 1000ml then, regulates pH6, as microbiological culture media.Inoculation saccharomyces cerevisiae shaken cultivation (200rpm) in shaking table in this fluid nutrient medium, 32 ℃ of cultivation temperature, incubation time 33h, the centrifugal 5min of 3000rpm obtains adsorbent.Add this adsorbent 5g/L, handle 15mg/L nickel liquid, shaking table with 180rpm velocity fluctuation 5h after with the centrifugal 7min of 3500rpm speed, clearance can reach 95%.
Embodiment 6: hunt moral sewage treatment plant from the Guangzhou and gather returned sludge, get above-mentioned mud 100g, add fructose, lactose 15g, peptone 5g altogether, making C/N is 5: 1, is settled to 1000ml then, regulates pH5, with this as microbiological culture media.In aforesaid liquid inoculation of medium shadow yeast shaken cultivation (150rpm) in shaking table, 37 ℃ of cultivation temperature, incubation time 35h, the centrifugal 10min of 4000rpm, collected bacterium mud is Biosorbent for heavy metals.Add this adsorbent 8g/L, handle 20mg/L copper liquid, shaking table with 90rpm velocity fluctuation 3h after with the centrifugal 5min of 3000rpm speed, clearance can reach 90%.
Embodiment 7: hunt moral sewage treatment plant from the Guangzhou and gather returned sludge, get above-mentioned mud 100g, add glucose, sucrose, lactose 20g altogether, yeast soaks powder 2g, and making C/N is 6: 1, is settled to 1000ml then, regulates pH7, as microbiological culture media.In this fluid nutrient medium, inoculate Candida lipolytica, shadow yeast, saccharomyces cerevisiae shaken cultivation (100rpm) in shaking table, 30 ℃ of cultivation temperature, incubation time 25h, the centrifugal 8min of 3500rpm obtains adsorbent.Add this adsorbent 6g/L, handle the 10mg/L chrome liquor, shaking table with 160rpm velocity fluctuation 5h after with the centrifugal 6min of 2800rpm speed, clearance can reach 98%.
Claims (5)
1. the preparation method of a Biosorbent for heavy metals is characterized in that, may further comprise the steps:
(1) add nutriment in residual active sludge, regulating C/N is 5: 1～8: 1, pH5～7, with this as microbiological culture media, with this as microbiological culture media;
(2) inoculation yeast is in 30～37 ℃ in the microbiological culture media that (1) obtains, and after 100～200rpm velocity fluctuation was cultivated 25～35h, the centrifugal 5～10min of 3000～4000rpm collected bacterium mud, and this bacterium mud is Biosorbent for heavy metals.
2. method according to claim 1, described nutriment comprise that glucose, fructose, sucrose, lactose, maltose, yeast soak powder, peptone, MgSO
4In a kind of or more than one.
3. method according to claim 1, described yeast are one or more in Candida lipolytica, shadow yeast, candida utili, the saccharomyces cerevisiae.
4. according to a kind of Biosorbent for heavy metals of the described method of one of claim 1～3 preparation.
5. according to the application of the described Biosorbent for heavy metals of claim 4 in handling heavy metal wastewater thereby, it is characterized in that, adsorbent is added in the heavy metal wastewater thereby, make adsorbent: the quality volumetric concentration ratio of heavy metal wastewater thereby is 5～10g/L: 10mg/L, shaking table with 50-200rpm velocity fluctuation 2～8h after with the centrifugal 5～10min of 2500～5000rpm speed.
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| CNA2007100310987A CN101229506A (en) | 2007-10-26 | 2007-10-26 | Heavy metal biological sorbent, preparing method and applications thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103230780A (en) * | 2013-04-19 | 2013-08-07 | 湖北工业大学 | Heavy-metal absorbent prepared from vinasse and preparation method for same |
| CN103570097A (en) * | 2013-11-08 | 2014-02-12 | 浙江师范大学 | Method for treating cationic dye wastewater through modified fall-off biofilm of biological filter |
| CN103864483A (en) * | 2014-02-27 | 2014-06-18 | 杨健 | Microorganism chelating agent for producing bio-organic fertilizer by removing organic solid waste heavy metal |
| CN105056908A (en) * | 2015-07-31 | 2015-11-18 | 邵素英 | Biological purifying carrier for wastewater treatment, and preparation method thereof |
| CN105876740A (en) * | 2016-04-19 | 2016-08-24 | 王胜 | Vegetable salt and method for preparing suaeda salsa vegetable salt by adopting microbial heavy-metal-removing technology |
| CN106947720A (en) * | 2017-04-17 | 2017-07-14 | 中南大学 | A kind of energy high-performance bio converts the microbial bacterial agent of Cadium In Polluted Soil form |
| CN107052042A (en) * | 2017-04-17 | 2017-08-18 | 湖南省农业生物技术研究中心 | A kind of application of the microbial bacterial agent of energy high-performance bio conversion Cadium In Polluted Soil form |
| CN109967040A (en) * | 2017-12-28 | 2019-07-05 | 营口富里泥炭科技有限公司 | A kind of preparation method of novel sludge compound adsorbent |
| CN112808304A (en) * | 2021-01-07 | 2021-05-18 | 昆明理工大学 | Preparation method and application of mediating substances for photodegradation of steroid estrogen |
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2007
- 2007-10-26 CN CNA2007100310987A patent/CN101229506A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103230780A (en) * | 2013-04-19 | 2013-08-07 | 湖北工业大学 | Heavy-metal absorbent prepared from vinasse and preparation method for same |
| CN103570097A (en) * | 2013-11-08 | 2014-02-12 | 浙江师范大学 | Method for treating cationic dye wastewater through modified fall-off biofilm of biological filter |
| CN103864483A (en) * | 2014-02-27 | 2014-06-18 | 杨健 | Microorganism chelating agent for producing bio-organic fertilizer by removing organic solid waste heavy metal |
| CN103864483B (en) * | 2014-02-27 | 2015-05-27 | 杨健 | Microorganism chelating agent for producing bio-organic fertilizer by removing organic solid waste heavy metal |
| CN105056908A (en) * | 2015-07-31 | 2015-11-18 | 邵素英 | Biological purifying carrier for wastewater treatment, and preparation method thereof |
| CN105876740A (en) * | 2016-04-19 | 2016-08-24 | 王胜 | Vegetable salt and method for preparing suaeda salsa vegetable salt by adopting microbial heavy-metal-removing technology |
| CN106947720A (en) * | 2017-04-17 | 2017-07-14 | 中南大学 | A kind of energy high-performance bio converts the microbial bacterial agent of Cadium In Polluted Soil form |
| CN107052042A (en) * | 2017-04-17 | 2017-08-18 | 湖南省农业生物技术研究中心 | A kind of application of the microbial bacterial agent of energy high-performance bio conversion Cadium In Polluted Soil form |
| CN106947720B (en) * | 2017-04-17 | 2020-04-24 | 中南大学 | Microbial agent capable of efficiently biotransforming cadmium form in polluted soil |
| CN107052042B (en) * | 2017-04-17 | 2020-04-28 | 湖南省农业生物技术研究中心 | Application of microbial agent capable of efficiently biotransforming cadmium form in polluted soil |
| CN109967040A (en) * | 2017-12-28 | 2019-07-05 | 营口富里泥炭科技有限公司 | A kind of preparation method of novel sludge compound adsorbent |
| CN112808304A (en) * | 2021-01-07 | 2021-05-18 | 昆明理工大学 | Preparation method and application of mediating substances for photodegradation of steroid estrogen |
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