CN101228957A - Method of preparing low molecular active substance pill of holothurian and oyster - Google Patents
Method of preparing low molecular active substance pill of holothurian and oyster Download PDFInfo
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- CN101228957A CN101228957A CNA2007100132744A CN200710013274A CN101228957A CN 101228957 A CN101228957 A CN 101228957A CN A2007100132744 A CNA2007100132744 A CN A2007100132744A CN 200710013274 A CN200710013274 A CN 200710013274A CN 101228957 A CN101228957 A CN 101228957A
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- sea cucumber
- oyster
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Abstract
The invention discloses a preparation method of a low molecular dropping pill with sea cucumber and oyster active materials; the invention is characterized by taking the sea cucumber or the oyster meat as the raw material, adopting the biological enzymolysis method, obtaining the sea cucumber enzymolysis liquid or the oyster enzymolysis liquid, correcting the flavor by the compound flavor protease, getting the low-molecular-weight active component liquid after ultrafiltrating the liquid after enzyme inactivation, adding an edible dropping pill matrix and getting the low molecular sea cucumber and the oyster active material dropping pill. The invention is made from the low-molecular active material and the utilization ratio of the active material is high. The low molecular sea cucumber and the oyster active material dropping pill is easy to be absorbed by the human body, rich in nutrient, reasonable in formulation and easy in administration.
Description
One, technical field
The invention belongs to marine organism health care product technical field, especially relate to the preparation method of a kind of low molecule sea cucumber, oyster active substance pill.
Two, background technology
At present, health food with sea cucumber or oyster preparation mostly is forms such as hard shell capsules, soft capsule, oral liquid, more than three kinds of forms to marine organisms such as sea cucumber and oysters, except that technology of preparing is single store and guarantee the quality, effect utilizes and all has a lot of deficiencies, such as: no matter be that physics method or enzymatic isolation method goods all do not have technical advantage in molecular weight control in the market 1,, influenced the performance and the absorption of effect; 2, owing to the problem content thing of dosage form very easily the moisture absorption lack the stability of long period; 3, on market owing to people produce trust crisis to the query of present formulation health products content, limited product development and utilization greatly.Sea cucumber and oyster are extensively approved as a kind of nourishment food, its main efficacy active component has confirmed to concentrate in the 1000-5000 Middle Molecular Substance, low molecular state bioavailability height, be absorbed by the body easily, the whole preparation process of dripping pill The Application of Technology and the present invention can fully solve the defective of these type of health products at present.
Three, summary of the invention
The objective of the invention is to improve the deficiency of prior art and provide a kind of and adopt that the low-molecular-weight active material is made, the active material utilization height, be absorbed by the body easily, nutritious, formulation rationally, the low molecule sea cucumber of taking convenience, the preparation method of oyster active substance pill.
The object of the present invention is achieved like this, the preparation method of a kind of low molecule sea cucumber, oyster active substance pill, be characterized in that this method is is raw material with sea cucumber or oyster meat, adopt biologic enzymolysis method, obtain sea cucumber hydrolysate or oysters enzymolysis liquid, carry out local flavor with composite flavor protease and correct, the liquid ultrafiltration behind the enzyme-deactivating obtains low-molecular-weight active component liquid, add edible dripping pill matrix, obtain low molecule sea cucumber, oyster active substance pill.
In order further to realize purpose of the present invention, the inventive method can be temperature below 25 ℃ with bright sea cucumber or bright oyster meat, clean, drain, break into magma by colloid mill, magma stirs in reactor and transfers pH value between 6-7.5, at constant temperature 40-60 ℃ of composite animal albumen enzyme that adds 0.5-1 ‰ down, enzymolysis time 1-3 hour, be warmed up to 70-95 ℃ and carry out enzyme-deactivating, the centrifuging and taking supernatant transfer pH value 5.5-7.5 temperature be 40-60 ℃ add 0.2-1 ‰ composite flavor protease after 2 hours intensification 70-95 ℃ carry out enzyme-deactivating, the centrifuging and taking supernatant ultrafiltration of cooling back, the rapid pre-freeze of supernatant after the ultrafiltration was to the distillation of final vacuum below-40 ℃ 24-48 hour, the freeze-dried powder that obtains is crossed 100 mesh sieves, dripping pill matrix is fused into liquid, in 50-80 ℃ of constant temperature, freeze-dried powder is slowly stirred in the 40% adding dripping pill matrix by the dripping pill gross weight in batches, splash in the condensing agent for preparing with the per minute kind 10-160 speed of dripping, collect dripping pill through centrifugal removal condensing agent, sub-active material of sea cucumber dripping pill and oyster peptide dripping pill promptly make low score.
Ultrafiltration obtains the molecular weight of low molecular activity component liquid below 3000.
Ultrafiltration behind the enzyme-deactivating is that sea cucumber hydrolysate is to remove the part salinity and collect molecular weight 3000 following active component liquid standby by the G25 gel filtration chromatography, and the oyster enzymolysis liquid is crossed behind 0.2 film standby by ultrafiltration.
Described edible dripping pill matrix is two or more mixing of poly-diethanol 6000, poly-diethanol 4000, polyoxyethylene 8 stearate fat, stearic acid, glycerine, gelatin, poloxamer, single stearic acid glycerine lipoprotein, beeswax.
Described condensing agent is one or more mixing materials of dimethicone, tea oil, liquid food wax.
Compared with the prior art the present invention has following distinguishing feature and good effect: the present invention adopts biologic enzymolysis method to extract active material active peptide, the holothurin mucopolysaccharide isoreactivity material of the ground molecular weight in sea cucumber, the oyster, the effective active composition that has farthest kept sea cucumber, oyster health-care efficacy, nutritious, be beneficial to absorption of human body, utilization, adopt the form of dripping pill matrix simultaneously, do not add any anticorrisive agent, be easy to carry, conveniently take.
Four, the specific embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1, a kind of low molecule sea cucumber, the preparation method of oyster active substance pill, be with bright sea cucumber vertically dissect gill and the chamber in foreign material, clean, drain, break into magma by colloid mill, above job requirement temperature is finished below 25 ℃, sea cucumber magma stirs in reactor and adjusts its pH value between 6-7.5, under 40-60 ℃ constant temperature, add 0.5-1 ‰ composite animal albumen enzyme, enzymolysis time 1-3 hour, be warmed up to 70-95 ℃ then and carry out enzyme-deactivating, the centrifuging and taking supernatant is also adjusted pH value between 5.5-7.5, be to add 0.2-1 ‰ composite flavor protease under the 40-60 ℃ of condition in temperature then, be warming up to 70-95 ℃ after 2 hours and carry out enzyme-deactivating, cooling back centrifuging and taking supernatant, remove the part salinity and collect molecular weight 3000 following active component liquid supernatant standby by the G25 gel filtration chromatography, the active component liquid that obtains after the ultrafiltration is cooled to-40 ℃ of freezing final vacuum distillations 24-48 hour rapidly, the freeze-dried powder that obtains is crossed 100 mesh sieves, in 50-120 ℃ of constant temperature, select poly-diethanol 6000, poly-diethanol 4000, polyoxyethylene 8 stearate fat, stearic acid, glycerine, gelatin, poloxamer, single stearic acid glycerine lipoprotein, two or more of beeswax fuses into liquid, present embodiment adopts poly-diethanol 6000 and beeswax to mix, in 50-80 ℃ of constant temperature, freeze-dried powder is slowly stirred by 40% adding of dripping pill gross weight in batches, drip speed by in the condensing agent for preparing with per minute kind 10-160, collect dripping pill through centrifugal removal condensing agent, sub-active material of sea cucumber dripping pill promptly makes low score, condensing agent is by dimethicone, tea oil, one or more mixing materials of liquid food wax, the present embodiment condensing agent adopts dimethicone.
Embodiment 2, the preparation method of a kind of low molecule sea cucumber, oyster active substance pill is on the basis of embodiment 1, and present embodiment adopts poly-diethanol 4000, polyoxyethylene 8 stearate fat, stearic acid to mix, condensing agent adopts tea oil and liquid food wax to mix, and other and embodiment 1 are identical.
Embodiment 3, the preparation method of a kind of low molecule sea cucumber, oyster active substance pill, be on the basis of embodiment 1, present embodiment adopts glycerine, gelatin, poloxamer, single stearic acid glycerine lipoprotein, beeswax to mix, condensing agent adopts dimethicone, tea oil, liquid food wax to mix, and other and embodiment 1 are identical.
Embodiment 4, a kind of low molecule sea cucumber, the preparation method of oyster active substance pill, be that bright oyster is removed shell and gastric pouch foreign material, tissue is smashed the back and is broken into magma by colloid mill, above job requirement temperature is finished below 25 ℃, oyster magma stirs in reactor and adjusts its pH value between 6-7.5, under 40-60 ℃ constant temperature, add 0.5-1 ‰ composite animal albumen enzyme, enzymolysis time 1-3 hour, be warmed up to 70-95 ℃ then and carry out enzyme-deactivating, the centrifuging and taking supernatant is also adjusted pH value between 5.5-7.5, be to add 0.2-1 ‰ composite flavor protease under the 40-60 ℃ of condition in temperature then, be warming up to 70-95 ℃ after 2 hours and carry out enzyme-deactivating, cooling back centrifuging and taking supernatant, cross 0.2 film after standby by ultrafiltration supernatant, the active component liquid that obtains after the ultrafiltration is cooled to-40 ℃ of freezing final vacuum distillations 24-48 hour rapidly, the freeze-dried powder that obtains is crossed 100 mesh sieves, in 50-120 ℃ of constant temperature, select poly-diethanol 6000, poly-diethanol 4000, polyoxyethylene 8 stearate fat, stearic acid, glycerine, gelatin, poloxamer, single stearic acid glycerine lipoprotein, two or more of beeswax fuses into liquid, present embodiment adopts poly-diethanol 4000 and gelatin to mix, in 50-80 ℃ of constant temperature, freeze-dried powder is slowly stirred by 40% adding of dripping pill gross weight in batches, drip speed by in the condensing agent for preparing with per minute kind 10-160, collect dripping pill through centrifugal removal condensing agent, sub-active material of sea cucumber dripping pill promptly makes low score, condensing agent is by dimethicone, tea oil, one or more mixing materials of liquid food wax, present embodiment condensing agent adopt liquid food wax.
Claims (6)
1. the preparation method of one kind low molecule sea cucumber, oyster active substance pill, it is characterized in that this method is is raw material with sea cucumber or oyster meat, adopt biologic enzymolysis method, obtain sea cucumber hydrolysate or oysters enzymolysis liquid, carrying out local flavor with composite flavor protease corrects, liquid ultrafiltration behind the enzyme-deactivating obtains low-molecular-weight active component liquid, adds edible dripping pill matrix, obtains low molecule sea cucumber, oyster active substance pill.
2. a kind of low molecule sea cucumber according to claim 1, the preparation method of oyster active substance pill, it is characterized in that temperature below 25 ℃ with bright sea cucumber or bright oyster meat, clean, drain, break into magma by colloid mill, magma stirs in reactor and transfers pH value between 6-7.5, at constant temperature 40-60 ℃ of composite animal albumen enzyme that adds 0.5-1 ‰ down, enzymolysis time 1-3 hour, be warmed up to 70-95 ℃ and carry out enzyme-deactivating, the centrifuging and taking supernatant transfer pH value 5.5-7.5 temperature be 40-60 ℃ add 0.2-1 ‰ composite flavor protease after 2 hours intensification 70-95 ℃ carry out enzyme-deactivating, the centrifuging and taking supernatant ultrafiltration of cooling back, the rapid pre-freeze of supernatant after the ultrafiltration was to the distillation of final vacuum below-40 ℃ 24-48 hour, the freeze-dried powder that obtains is crossed 100 mesh sieves, dripping pill matrix is fused into liquid, in 50-80 ℃ of constant temperature, freeze-dried powder is slowly stirred in the 40% adding dripping pill matrix by the dripping pill gross weight in batches, splash in the condensing agent for preparing with the per minute kind 10-160 speed of dripping, collect dripping pill through centrifugal removal condensing agent, sub-active material of sea cucumber dripping pill and oyster peptide dripping pill promptly make low score.
3. the preparation method of a kind of low molecule sea cucumber according to claim 1 and 2, oyster active substance pill is characterized in that ultrafiltration obtains the molecular weight of low molecular activity component liquid below 3000.
4. the preparation method of a kind of low molecule sea cucumber according to claim 1 and 2, oyster active substance pill, it is characterized in that ultrafiltration behind the enzyme-deactivating is that sea cucumber hydrolysate is to remove the part salinity and collect molecular weight 3000 following active component liquid standby by the G25 gel filtration chromatography, the oyster enzymolysis liquid is crossed behind 0.2 film standby by ultrafiltration.
5. the preparation method of a kind of low molecule sea cucumber according to claim 1 and 2, oyster active substance pill is characterized in that described edible dripping pill matrix is two or more mixing of poly-diethanol 6000, poly-diethanol 4000, polyoxyethylene 8 stearate fat, stearic acid, glycerine, gelatin, poloxamer, single stearic acid glycerine lipoprotein, beeswax.
6. the preparation method of a kind of low molecule sea cucumber according to claim 2, oyster active substance pill is characterized in that described condensing agent is one or more mixing materials of dimethicone, tea oil, liquid food wax.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007100132744A CN101228957B (en) | 2007-01-24 | 2007-01-24 | Method of preparing low molecular active substance pill of holothurian and oyster |
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| CN2007100132744A CN101228957B (en) | 2007-01-24 | 2007-01-24 | Method of preparing low molecular active substance pill of holothurian and oyster |
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| CN101228957A true CN101228957A (en) | 2008-07-30 |
| CN101228957B CN101228957B (en) | 2011-02-09 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102174073A (en) * | 2010-12-17 | 2011-09-07 | 中国海洋大学 | Oyster protein peptide and zinc chelate and method for preparing same |
| CN102488074A (en) * | 2011-12-29 | 2012-06-13 | 吴庆林 | Method for extracting oyster peptide |
| CN101836746B (en) * | 2009-03-16 | 2013-08-07 | 广东中大南海海洋生物技术工程中心有限公司 | Method for extracting polypeptide by enzymolysis on oyster at low temperature |
| CN103724442A (en) * | 2013-12-06 | 2014-04-16 | 山东好当家海洋发展股份有限公司 | Preparation method for low-protein and high-purity sea cucumber polysaccharide |
| CN105200105A (en) * | 2015-09-10 | 2015-12-30 | 烟台源力德海洋生物有限公司 | Preparation method of oyster protein liver protecting peptide preparation |
| CN105296584A (en) * | 2015-10-09 | 2016-02-03 | 烟台源力德海洋生物有限公司 | Preparation method of marine biological health care peptide preparation |
| CN111139278A (en) * | 2020-03-05 | 2020-05-12 | 山西原生肽科技有限公司 | Method for extracting small molecule peptide from sea cucumber and application thereof |
| CN112680492A (en) * | 2020-12-04 | 2021-04-20 | 广州天启生物科技有限公司 | Fishy smell-free low-molecular-weight oyster peptide and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1274249C (en) * | 2004-08-27 | 2006-09-13 | 宁波大学 | Method for producing oyster biological active substance and its product |
| CN1871945B (en) * | 2005-06-01 | 2010-05-26 | 王伟成 | Method for distilling freeze-dried instant powder from active substance of sea cucumber |
-
2007
- 2007-01-24 CN CN2007100132744A patent/CN101228957B/en active Active
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101836746B (en) * | 2009-03-16 | 2013-08-07 | 广东中大南海海洋生物技术工程中心有限公司 | Method for extracting polypeptide by enzymolysis on oyster at low temperature |
| CN102174073A (en) * | 2010-12-17 | 2011-09-07 | 中国海洋大学 | Oyster protein peptide and zinc chelate and method for preparing same |
| CN102488074A (en) * | 2011-12-29 | 2012-06-13 | 吴庆林 | Method for extracting oyster peptide |
| CN102488074B (en) * | 2011-12-29 | 2013-08-07 | 吴庆林 | Method for extracting oyster peptide |
| CN103724442A (en) * | 2013-12-06 | 2014-04-16 | 山东好当家海洋发展股份有限公司 | Preparation method for low-protein and high-purity sea cucumber polysaccharide |
| CN105200105A (en) * | 2015-09-10 | 2015-12-30 | 烟台源力德海洋生物有限公司 | Preparation method of oyster protein liver protecting peptide preparation |
| CN105296584A (en) * | 2015-10-09 | 2016-02-03 | 烟台源力德海洋生物有限公司 | Preparation method of marine biological health care peptide preparation |
| CN111139278A (en) * | 2020-03-05 | 2020-05-12 | 山西原生肽科技有限公司 | Method for extracting small molecule peptide from sea cucumber and application thereof |
| CN112680492A (en) * | 2020-12-04 | 2021-04-20 | 广州天启生物科技有限公司 | Fishy smell-free low-molecular-weight oyster peptide and preparation method thereof |
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|---|---|
| CN101228957B (en) | 2011-02-09 |
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Effective date of registration: 20160819 Address after: 265600, 25, Dongguan Road, Penglai, Shandong, Yantai Patentee after: YANTAI YUANLIDE MARINE BIOLOGICAL CO., LTD. Address before: Dengzhou town Penglai city Shidao village 265600 Shandong city of Yantai province No. 323 Patentee before: Wang Weicheng |